LOCUS       DADVXO010000001        76030 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.001, whole genome shotgun sequence.
ACCESSION   DADVXO010000001 DADVXO010000000
VERSION     DADVXO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 76030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.001"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(350..829)
                     /gene="rlmH"
                     /locus_tag="KB950_000001"
     CDS             complement(350..829)
                     /gene="rlmH"
                     /locus_tag="KB950_000001"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBB6856248.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1257..2546
                     /locus_tag="KB950_000002"
     CDS             1257..2546
                     /locus_tag="KB950_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBB6856249.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2585..3190)
                     /locus_tag="KB950_000003"
     CDS             complement(2585..3190)
                     /locus_tag="KB950_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBB6856250.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3395..3859)
                     /locus_tag="KB950_000004"
     CDS             complement(3395..3859)
                     /locus_tag="KB950_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6856251.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3881..4201)
                     /locus_tag="KB950_000005"
     CDS             complement(3881..4201)
                     /locus_tag="KB950_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBB6856252.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4345..5424)
                     /gene="pepA"
                     /locus_tag="KB950_000006"
     CDS             complement(4345..5424)
                     /gene="pepA"
                     /locus_tag="KB950_000006"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBB6856253.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5624..5974
                     /locus_tag="KB950_000007"
     CDS             5624..5974
                     /locus_tag="KB950_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBB6856254.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6197..7144
                     /locus_tag="KB950_000008"
     CDS             6197..7144
                     /locus_tag="KB950_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6856255.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7201..8619)
                     /gene="pepV"
                     /locus_tag="KB950_000009"
     CDS             complement(7201..8619)
                     /gene="pepV"
                     /locus_tag="KB950_000009"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBB6856256.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8768..9613
                     /locus_tag="KB950_000010"
     CDS             8768..9613
                     /locus_tag="KB950_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBB6856257.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9617..10057
                     /locus_tag="KB950_000011"
     CDS             9617..10057
                     /locus_tag="KB950_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBB6856258.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10107..10301
                     /locus_tag="KB950_000012"
     CDS             10107..10301
                     /locus_tag="KB950_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856259.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10437..11114
                     /locus_tag="KB950_000013"
     CDS             10437..11114
                     /locus_tag="KB950_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6856260.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(11522..12211)
                     /locus_tag="KB950_000014"
     CDS             complement(11522..12211)
                     /locus_tag="KB950_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856261.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12186..14315)
                     /locus_tag="KB950_000015"
     CDS             complement(12186..14315)
                     /locus_tag="KB950_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBB6856262.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14363..15700)
                     /locus_tag="KB950_000016"
     CDS             complement(14363..15700)
                     /locus_tag="KB950_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBB6856263.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(15952..16311)
                     /gene="rplT"
                     /locus_tag="KB950_000017"
     CDS             complement(15952..16311)
                     /gene="rplT"
                     /locus_tag="KB950_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBB6856264.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16364..16564)
                     /gene="rpmI"
                     /locus_tag="KB950_000018"
     CDS             complement(16364..16564)
                     /gene="rpmI"
                     /locus_tag="KB950_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBB6856265.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16636..17160)
                     /gene="infC"
                     /locus_tag="KB950_000019"
     CDS             complement(16636..17160)
                     /gene="infC"
                     /locus_tag="KB950_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBB6856266.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17204..17329)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17480..18112
                     /locus_tag="KB950_000020"
     CDS             17480..18112
                     /locus_tag="KB950_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB6856267.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18114..19019)
                     /locus_tag="KB950_000021"
     CDS             complement(18114..19019)
                     /locus_tag="KB950_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6856268.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19104..19763)
                     /locus_tag="KB950_000022"
     CDS             complement(19104..19763)
                     /locus_tag="KB950_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6856269.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            20079..21023
                     /gene="mvk"
                     /locus_tag="KB950_000023"
     CDS             20079..21023
                     /gene="mvk"
                     /locus_tag="KB950_000023"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBB6856270.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21016..21993
                     /gene="mvaD"
                     /locus_tag="KB950_000024"
     CDS             21016..21993
                     /gene="mvaD"
                     /locus_tag="KB950_000024"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBB6856271.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22006..23091
                     /locus_tag="KB950_000025"
     CDS             22006..23091
                     /locus_tag="KB950_000025"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBB6856272.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23088..24152
                     /locus_tag="KB950_000026"
     CDS             23088..24152
                     /locus_tag="KB950_000026"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBB6856273.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24401..25204)
                     /locus_tag="KB950_000027"
     CDS             complement(24401..25204)
                     /locus_tag="KB950_000027"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBB6856274.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25224..26066)
                     /gene="kduI"
                     /locus_tag="KB950_000028"
     CDS             complement(25224..26066)
                     /gene="kduI"
                     /locus_tag="KB950_000028"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBB6856275.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26082..27089)
                     /locus_tag="KB950_000029"
     CDS             complement(26082..27089)
                     /locus_tag="KB950_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBB6856276.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27096..27740)
                     /locus_tag="KB950_000030"
     CDS             complement(27096..27740)
                     /locus_tag="KB950_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBB6856277.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(27840..28598)
                     /locus_tag="KB950_000031"
     CDS             complement(27840..28598)
                     /locus_tag="KB950_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBB6856278.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28725..29876)
                     /gene="fucO"
                     /locus_tag="KB950_000032"
     CDS             complement(28725..29876)
                     /gene="fucO"
                     /locus_tag="KB950_000032"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBB6856279.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(29958..30269)
                     /gene="rhaM"
                     /locus_tag="KB950_000033"
     CDS             complement(29958..30269)
                     /gene="rhaM"
                     /locus_tag="KB950_000033"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBB6856280.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30271..31116)
                     /gene="rhaD"
                     /locus_tag="KB950_000034"
     CDS             complement(30271..31116)
                     /gene="rhaD"
                     /locus_tag="KB950_000034"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBB6856281.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31130..32386)
                     /gene="rhaA"
                     /locus_tag="KB950_000035"
     CDS             complement(31130..32386)
                     /gene="rhaA"
                     /locus_tag="KB950_000035"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBB6856282.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32383..33843)
                     /gene="rhaB"
                     /locus_tag="KB950_000036"
     CDS             complement(32383..33843)
                     /gene="rhaB"
                     /locus_tag="KB950_000036"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBB6856283.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            33996..34865
                     /locus_tag="KB950_000037"
     CDS             33996..34865
                     /locus_tag="KB950_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBB6856284.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(34991..35890)
                     /locus_tag="KB950_000038"
     CDS             complement(34991..35890)
                     /locus_tag="KB950_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6856285.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36055..36879)
                     /locus_tag="KB950_000039"
     CDS             complement(36055..36879)
                     /locus_tag="KB950_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6856286.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(36890..38185)
                     /locus_tag="KB950_000040"
     CDS             complement(36890..38185)
                     /locus_tag="KB950_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBB6856287.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38201..38680)
                     /locus_tag="KB950_000041"
     CDS             complement(38201..38680)
                     /locus_tag="KB950_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBB6856288.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38686..39714)
                     /locus_tag="KB950_000042"
     CDS             complement(38686..39714)
                     /locus_tag="KB950_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBB6856289.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39731..40837)
                     /locus_tag="KB950_000043"
     CDS             complement(39731..40837)
                     /locus_tag="KB950_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBB6856290.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41066..43663)
                     /gene="adhE"
                     /locus_tag="KB950_000044"
                     /gene_synonym="adhC"
     CDS             complement(41066..43663)
                     /gene="adhE"
                     /locus_tag="KB950_000044"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBB6856291.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(43968..44348)
                     /gene="yajC"
                     /locus_tag="KB950_000045"
     CDS             complement(43968..44348)
                     /gene="yajC"
                     /locus_tag="KB950_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBB6856292.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44445..45590)
                     /gene="tgt"
                     /locus_tag="KB950_000046"
     CDS             complement(44445..45590)
                     /gene="tgt"
                     /locus_tag="KB950_000046"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBB6856293.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            45808..46506
                     /locus_tag="KB950_000047"
     CDS             45808..46506
                     /locus_tag="KB950_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBB6856294.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46674..49313)
                     /locus_tag="KB950_000048"
     CDS             complement(46674..49313)
                     /locus_tag="KB950_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6856295.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(49932..50963)
                     /gene="queA"
                     /locus_tag="KB950_000049"
     CDS             complement(49932..50963)
                     /gene="queA"
                     /locus_tag="KB950_000049"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBB6856296.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            51156..52331
                     /locus_tag="KB950_000050"
     CDS             51156..52331
                     /locus_tag="KB950_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6856297.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52335..52976
                     /locus_tag="KB950_000051"
     CDS             52335..52976
                     /locus_tag="KB950_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856298.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            52973..53890
                     /locus_tag="KB950_000052"
     CDS             52973..53890
                     /locus_tag="KB950_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6856299.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            53887..54558
                     /locus_tag="KB950_000053"
     CDS             53887..54558
                     /locus_tag="KB950_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856300.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(55099..55587)
                     /locus_tag="KB950_000054"
     CDS             complement(55099..55587)
                     /locus_tag="KB950_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBB6856301.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(55788..56054)
                     /locus_tag="KB950_000055"
     CDS             complement(55788..56054)
                     /locus_tag="KB950_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6856302.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(56054..56317)
                     /locus_tag="KB950_000056"
     CDS             complement(56054..56317)
                     /locus_tag="KB950_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6856303.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            56793..56978
                     /locus_tag="KB950_000057"
     CDS             56793..56978
                     /locus_tag="KB950_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6856304.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(57120..58943)
                     /locus_tag="KB950_000058"
     CDS             complement(57120..58943)
                     /locus_tag="KB950_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBB6856305.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59541..62246)
                     /locus_tag="KB950_000059"
     CDS             complement(59541..62246)
                     /locus_tag="KB950_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBB6856306.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62552..63499)
                     /locus_tag="KB950_000060"
     CDS             complement(62552..63499)
                     /locus_tag="KB950_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBB6856307.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            63689..63958
                     /gene="rpsN"
                     /locus_tag="KB950_000061"
     CDS             63689..63958
                     /gene="rpsN"
                     /locus_tag="KB950_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBB6856308.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(64134..64496)
                     /locus_tag="KB950_000062"
     CDS             complement(64134..64496)
                     /locus_tag="KB950_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6856309.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64507..65628)
                     /locus_tag="KB950_000063"
     CDS             complement(64507..65628)
                     /locus_tag="KB950_000063"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBB6856310.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65644..65994)
                     /locus_tag="KB950_000064"
     CDS             complement(65644..65994)
                     /locus_tag="KB950_000064"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBB6856311.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(66124..67635)
                     /locus_tag="KB950_000065"
     CDS             complement(66124..67635)
                     /locus_tag="KB950_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6856312.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(67957..69309)
                     /locus_tag="KB950_000066"
     CDS             complement(67957..69309)
                     /locus_tag="KB950_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBB6856313.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69462..70538)
                     /locus_tag="KB950_000067"
     CDS             complement(69462..70538)
                     /locus_tag="KB950_000067"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBB6856314.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            70734..72479
                     /locus_tag="KB950_000068"
     CDS             70734..72479
                     /locus_tag="KB950_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBB6856315.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(72759..73196)
                     /locus_tag="KB950_000069"
     CDS             complement(72759..73196)
                     /locus_tag="KB950_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBB6856316.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(73180..74553)
                     /locus_tag="KB950_000070"
     CDS             complement(73180..74553)
                     /locus_tag="KB950_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856317.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(74875..75363)
                     /locus_tag="KB950_000071"
     CDS             complement(74875..75363)
                     /locus_tag="KB950_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6856318.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75385..75987)
                     /locus_tag="KB950_000072"
     CDS             complement(75385..75987)
                     /locus_tag="KB950_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6856319.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21791 a        16252 c        13913 g        24074 t
ORIGIN      
        1 aagctaaaaa attactatgt cttatttcat ttttagatag cttaagtatc tgaaatatat
       61 ttgttttccc agtccattta gatagttttc ttcaatatca tccatatcct ctaatagatt
      121 ttttaaacac tttttttcat ctatcatgtt ttttctcctt gccattattc taactaaaat
      181 tttacgtatt tgtttctatg tctacattat ctttttaaca gtatctattt ttcttttgca
      241 acttacttgg ctaccaaagc aaataaaaaa gttatttata aatttctccc taccactacc
      301 tattgctatt atttaaaata gggataattt tttctcttaa aaaatatcat tatttgtggt
      361 atggagcccc cgcattgatc cggaaggcac ggtagatttg ctcgacaagg atcagacgca
      421 tcaattgatg tggatacgtc atttttccaa aagagatctg agcattgctt cttttcatga
      481 cggcttcgct gagtcctaaa gagccgccga taacaaaaac aaggtggctt tttccttgga
      541 tacctaattg gtcgatttgt ttagcaaaag attcacttgt aggatttttc ccttcaattg
      601 ctaaggcata gacgtattct tgttctttga ttttcgctaa gatccgttcc ccttcttttt
      661 cttttacttg tctcatttgc gcttcgctta aattctccgg ggctttttcg tcaggaactt
      721 cgatcaattc gatttttgca taggcattca gccgtttgac atactcattg ataccttgga
      781 tcaggtattt ttcttttaat tttcctactg agataatttt gatattcatg ttttctctcc
      841 tttggtcttc atgctcactt tgtttattat aatcaatttt ttcgtctata tgtttatttt
      901 ggctattttt gttaaaaaga tttccccaac agttatgcac aacatccaca ggtttatcca
      961 cagaaaacag tgttatttta ccttatttca tcacattcat taggcaaaca tacgttttat
     1021 aagaatttat ccactttata cacaggctgt ggatatgttt tatcccttat ttttccaatc
     1081 ctatccgcgt agttttacac agtatctgtg gaaaaagaaa atcaaatatt ttcatctgaa
     1141 aacaatgatt tttcctaaag tttttcataa aaagttcaca gtctcaaagt agactaaagt
     1201 caacaaataa gactatacta acagtagata aatgaatgta attggaggag gaacacatgg
     1261 atagaaaaaa tgtgacacca aagatgaaaa aaagtaaaaa taatagcctc tggcgtaagt
     1321 taggtctggg gcttgttgga gggatcgttg gaggactact gacagctggc attttttatg
     1381 cagcgatggg cactggcaac aatagttcta ctgctacaag cggcaatcag aattctgccg
     1441 gtgaaacagt tgtggaaaat gtcaaagtca atgtagattc tgatatcacc aaagcggtag
     1501 ataaagttca agacgctgtc gtttctgtta tcaacttaca aagtcaaaac caatcaagcg
     1561 gttttggcgg actattcggg cagcaagaag aaagtagcag ctcagatgat agtaatttgg
     1621 aagcttatag tgaaggaagc ggcgtgatct acaaaaaaga cgggaatact gcttatgtag
     1681 tcacgaataa tcacgtagta gatggccagc aaggtcttga agtcttgatg aaagacggta
     1741 cgaaggtaaa agctgaatta gttggaacag atgcttattc agatttagcg gtgttgaaga
     1801 tcaattcaga caaagtcgaa acagttgcct cttttggtga ctcaagtgct ctgaaggttg
     1861 gtgagcctgc gattgcgatt ggttccccac taggatctga atatgcaaat tctgtcactt
     1921 caggaatcat ttcttccttg aaccgacaag tgacaagtac gaatgaatcg aacgaaacag
     1981 tcaatatcaa tgcgatccaa acagatgcag caatcaaccc tggaaattct ggtggtcctt
     2041 tagttaatat cgaaggacaa gttatcggta tcaactcaag taagatcgca agtacttctg
     2101 aatctacttc taatgtaagc gtagaaggaa tgggatttgc tattccaagt aacgatgtag
     2161 tgaatatcat caatcaactg gaaaaagacg gaaaagtgac acgtccagct ctaggtatca
     2221 caatggtcga cttatctgcc gtttctactc agcaacaaga acaaatcttg aagattcctt
     2281 catctgtaac taacggtgtc atcgtgacaa atgtccaaac agctacaccg gctgaaaaag
     2341 ctggattgaa acaatacgac gtcattacta agatcgatga caaagaagtt tcttctggtg
     2401 tagaacttca atctgctttg tataagaaaa aagtgggaga ttctgtcaaa atcacttact
     2461 atgaaggctc gaaagaaaaa acaactactg ttgaattgac gatcgatcaa tcggcattaa
     2521 aacaaaatca aaatagtgaa aattaataaa aaagaggttg tgacaaagac ttgtcacaac
     2581 ctctttattt tggaaatgct tctccgacta cggcatcctc aggcaatact aagattcctt
     2641 tttttgctgg agcatttggc aaatgcaatt cacgagcgga acagatcatt ccatagctat
     2701 ctacccctcg caaagttcct ggccagatca tcattccatc tggcatcata gctcctggtt
     2761 ttgcaacgac aactttttgt ccagcttcga tattgggtgc accgcaaacg atttgcaata
     2821 ctgtaccatt gtctacttct gtctcagtaa tcgataaatg gtcagaatca ggatgtggga
     2881 cgcatgtttt tacgtacccg acaacgatct tcggttcatg atcagcttca agctgttctt
     2941 cgaatcctgc ttctttgatc aatcgattca atttcgctaa ttcctcatct gttaagctta
     3001 cttggccgtt cccttcgatt gttagatgat cggagatatg gaaaaagttc caagcaacaa
     3061 cttgaccttg ttcatctttg atccgagcaa cattccattt gcgttcaaca gtgttttctg
     3121 ttcctttatc gtcggctata ataaccatca aggtgtctcc gacgtgtgct ttgttataag
     3181 caaaaatcat gttctttcct ccattatcct ttgctgagat ttatcttaac aaaaaattgc
     3241 attcactgca tgaaattttt ttcatgcttt atttccgaag aagcctcttc tggaacacca
     3301 tttattcgta acaagggact gtgacaagac ttttgtcaca gtccctttac tgttttagtt
     3361 aattgattaa aaaggtcgta caagttccac ttgcttaatt acttggctgt tcttcttcgt
     3421 cagaaacgat caagacatca caaggtgaat gacgtatcac gtagctggct acacttcctg
     3481 tcataaagcg ttcgactgca tttagacctg actgcccaac catgattaga tcgatctcat
     3541 atttttttgg caatttgact gccatggatt cacgaacaga accatatgca ataattcctt
     3601 ctatatttgt gtaatcaaca gatttcccat attctttaca ttcttcgatc atttccttgg
     3661 ctgtttcagt ttgctgatcg actagatttt gatcaatagg tgcatagccc ataaagttat
     3721 aaaattgctg atcgattacg tttgctacat atactttccc attattacgg cgagccactt
     3781 ctaccgcttt tttgaaagca gcaagtgctt gttcactacc gtcgatacct actaaaatat
     3841 tcttatattc ttgattcatc ttgacccctc ctaacagatg tcagcctttt gttacacttt
     3901 ctaggaacga ttcgatttcc tgttttgttt tacgatcttt attcactaga cgacctaatt
     3961 cttttccgtc ttggatcacg acaaaacttg ggataccaaa aatgttccat tcagcggcta
     4021 catctatgta cttgtcacgg tctacttcaa taaattgcca atcagaaaag tcattttcaa
     4081 tttcaggcat ttgcggtttg ataaaacggc agtctccgca ccagccggca gtaaagaaaa
     4141 atacactctt tccagtttct acgtagcctg ctaattcttc taaactattt gggataatca
     4201 ttctttgtcc tctactttct gtttatgtat ttttcaatta aattatacac ctttctttcg
     4261 aactaacaaa cattgatcct ctttttataa aaaaaatctg gcaaaaagca atcttttgcc
     4321 agattttata gtttcactat cgcattattt tccgtatacg atcgtattta ctgttgattt
     4381 atccaaacct ttcagtgttt gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact
     4441 gaacatcgtt tgatgcgtat ggatataacg tcccactacg ccaatcactg tacttgggat
     4501 tccttcgttt tgtgtatgag cagcacctgc atctgtaccg ccttttgaaa caaagtattg
     4561 ataagggata ttgtttgttt cagcgatatc cagcaagtac tcgcgcaaac gaggaagcat
     4621 gatcaaacct ggatcttgga tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc
     4681 atttttcgtc actgtatcat ctgctgcaga gcagtctact gcaaagaaca gatctggctt
     4741 gaatttattg acagaagctt tagaaccacg caagcctact tcttcctgga cattcgcgcc
     4801 tgcaatcaat gtatgaccta atgtttcatt ttgcaacgct tctaaagctt ctaataccat
     4861 tgtacagcca tagcggttgt cccatgattt actgataata ttctttccgt tggctgtttt
     4921 gatcgtttca gcgtaaggaa cgattgtgtc tcctggaagg acaccgtaac tcattgcctc
     4981 ttctcttgat tcaaacccag catcgaacaa aatatcggtt acttctactg atttttgtcc
     5041 gcttgttcct cgcaataaat gtgggggaac ggaagaagaa atacatggat aattcttacc
     5101 agtagaggtt ttaagtgtaa aacgctgagc agaaacaacg taaggattcc agccgcctag
     5161 aggtacgact ttaaaaagcc cgttttcttg gatttgtgtc agcatgaatc cgacttcatc
     5221 catatgtgct gcgaccatta cacggggtgc atccgcttct tcatgatggc gcagaccaaa
     5281 aatgccgccc aaaccatcta actgtaactc atctaccagc ggttccatat gactttccat
     5341 ataagcgcga acatcttgtt caaaaccgct tgttccttgt aattctgtta actctttgat
     5401 tcgttgaaat gttttttctt ccatatgcta ttcgtctaaa tttcagacta aactcctctc
     5461 agacttatat tgatctgcgg ataaaatatg attttttctg cagaccgatc gtatttatta
     5521 tagcttattt tttctggtag cggttattta tttcaaagaa aatatgagag tgtggtaaaa
     5581 taagcacaag gagtaacaag tgagaagaag gaggtatgtc cacatgtatg aagaaagcga
     5641 actaagctat tttaaaggcg ggttagcagt tggaatcggt ctaggattat tgagcggggt
     5701 agcttcaacg atttggtatc atcgcaaaaa agtattgtca gcagaccaag tacttgaaaa
     5761 tatcaagtca tcttttctaa aagaaggtgc aatcgaaggt tcttggatct cttttgaaaa
     5821 ggaacctatt aggaaattcg ccgttcggtc gagtgcttat cggggaggtc tttctcgaat
     5881 agaagatggc gaaactgttt attatgaatt tttagcagat gctcatacag gaaccgtatt
     5941 agaaatttca agaaaaaaag gtgcaacaga ttaatacgtc aaaaaagtga gagaacgaat
     6001 acgcacccac tattcaaaag agaggtatca tgtccttctt ttgtagtggg tggtttttta
     6061 gatagagctt tccaaacata tattccagta ttttcttttt aaataaatga tatttagcgg
     6121 gaaaaactag catttccttt gattatgagg tatttttaat ttagcttttg ttttctatag
     6181 ttaaggagag tgaaaaatgg aacaacaaga aaaaattgct gttctgattc cttgttacaa
     6241 tgaggaagca acaatttcaa cagtcattgc agactttaaa cgagaattac cagaagcaga
     6301 tatttatgtc tatgataata attcgaccga tcgaacatac gaattggccg ttcaaggtgg
     6361 agcaatcgta aaaaaagaac ctcgtcaagg aaaaggaaat gtgatccgcc aaatgttctt
     6421 tgatatcgat gccgactatt atttaatggt agatggcgat gatacttatc ctgcagaagc
     6481 tgtacatggt ttgctcgaga aattacgttc tggtgaagca gatatggtca ttggtgatcg
     6541 cctatcaaat ggaacctact ttgatgagaa taagagacct ttccacgatt ttggaaataa
     6601 ccttgttaga aatacaatta ctagaatgta taaaacaaaa attcttgatg tcatgaccgg
     6661 ctatcgagga ttcaatcgta tctttgtcaa aagttttcct attatgagtt ctggttttca
     6721 aatcgaaacg gaattgacga tccatgcttt agataaaaaa ttcaagttcg ttgaaatccc
     6781 aatcgattat cgtgatcgtc ctgaaggcag cgaatcaaaa ttgaatacat tctctgacgg
     6841 attcaaagtg atcatgatga ttattaaaat gtttaaagat tataagcctt tgcttttctt
     6901 tgggatttgg accatcttct tctttttatt tggattggtc acggggattc ctgtgattcg
     6961 cgaatatatg ctgacaagct ttattacacg catcccttct gcgattttaa gtactggatt
     7021 gatgacttta gcacttcttt cattagtaac agggttgatt ttggatacag tcgtcacgaa
     7081 cgctaagaaa gaatatgaat tgaatctata tcatgtttat catgaatacc acagtagaaa
     7141 gtaaaaaaag gatcaagact gcaaaaataa tttgcaattc ttgatcctct ttttaattaa
     7201 ttatttcgcc aaacgataga tggcatctgc atagattgct gttgctctga acaagtcatc
     7261 aagactcata aattcgtttg cttgatgcat tgtatcagta tatcctggga acatcgctcc
     7321 ataagcaacg ccacgtttca acaaacgtcc gtaagttccg ccaccgatga tttgttcgta
     7381 gcctttttct ccagtgtggt cttcatagac ttgcaataat gtagccacca atggatcatc
     7441 cattggtacg taatgtggtt ccattacgcg agcacctcga gtaactgttg cgccttcttg
     7501 ccccactgtt tgttcgatgt cactttgcaa tccgtctact gttacacctt ttggaaaacg
     7561 gaaattcata ttgatgaagt tcgcttcctc tgtaccattt tcttcaaaag caaatagtcc
     7621 agcattcatt gtcaatttgc ccattttttc atcttcataa gcgactccta gtttttctcc
     7681 ataaaaatct tcatggacat aagcagctgc tacatggata aattgtttag cagatcctag
     7741 gaattcatag ttatctaaga acaatgccaa gaaagaacct gcattgatac cagattgtgg
     7801 gcttgcacca tgtgcgcctt taccaactaa ttcgattttt acatacgtat tgtccgcttc
     7861 aatcgttccg cttactggat ttgcttcgat gaattggtaa aatgcttctt ccatcgcgat
     7921 agctgcatct gcacttggta cttgaagtgc tgcagttgct gttcctggca ccatattttc
     7981 gcgaagtccg gaaacaaaag atttcaagac ataatctcct gcattgtcgc ctttgaagcg
     8041 taaagctaaa gaaacatttc ctttttcacc attgatgatt gggaattcag catctggtga
     8101 gaaaccaaaa tctggttctt cttcatgttc gaaataataa gccatatcgc cccagccgct
     8161 ttcttcatcg ctgccgacta cgaagcgaac tttttttgat agtttcaagt caagttcttt
     8221 aatgatcttc atcgcatagt aagctgccat acttggacct ttgtcatcgc ttgatccgcg
     8281 tgcaaagatc tttccatcct tgattactgg ttcataagga tctgtgtccc agccatctcc
     8341 tgcaggaacg acatccatgt gtccaaatat ccctagcgtt tcgtctcctt cgccaagatc
     8401 aatatgccct gcatagttat cgacattttt tactttaaat ccatcacgtt cgccataagc
     8461 taacatatgt ttcaatgcgt cgcgaggtcc aggtccaaat ggtgcatctg gtgttacttt
     8521 tgagtcatcg cgctcgctgt tgacacgtaa aagatttttc aagtcttcaa gcaagtcttc
     8581 tttacgtgct tctacttctt tttgccaatc aattgtcata aagttaatcc tccacttatt
     8641 taagtcttcg tgttcatgat accacaaatt acagaaaaaa atagttcaaa aaatctgaca
     8701 attccttttc ttatctgttt ttctctaaaa aaaatatacg ctataatgag taaaaggagg
     8761 tttttagatg ctgataaacg aacaactagt gaaaagagtc atcacacctc gtccaaaaat
     8821 ttcacataaa ggaacatttg gacgtatcgt tttgattggc ggcaatgaac aatatggcgg
     8881 agcaatcatt atgagtgctt tagctgccgt acacagcgga gcgggtctca cgactgtagt
     8941 aactgatgaa aaaaatcgga gtgctcttca cagtcatctg ccagaagcaa tgattgtagt
     9001 ttggacggat actgaccagt ttctttccgt aataaagcaa gcagatgtac tcttgattgg
     9061 tcccggattg ggtacttctt ctaagagttt ggaacgtttc aaacaagtac tagaaaacca
     9121 atcaaaagaa caatggctgg tgattgatgg ctctgcttta agtttacttg ctgctaatca
     9181 tcttttgcta ccctatcccg aacaaactgt acttacacct catcaaatgg agtggcaacg
     9241 gttgagttct ctaccgatac aagaacaacg ggataaagaa aacaaaaaga agcaacagga
     9301 aatcggtgct gtgctcgttt tgaaaagtca tgagacaaaa atttatggtc accagcactt
     9361 ctacaccaat cctttaggtt ctgctgctat ggcaacgggc ggcatgggtg acactttagc
     9421 tgggatgatc acaggatttt tagcccagtt tcctcaaaat gaccaaacga ttgcagcagc
     9481 tatttatctt catagtttca ttggagacca gctggcaaaa gaacaatata ttgttttgcc
     9541 gacacaaatc agcgaagcac ttccgaaatg gatgaattat ttttgtaaaa aatcaatgga
     9601 aaacgaggca tgagtcatgg agaaatttac aggttatgtg cgagaacctt tttattatga
     9661 aacagatcaa atggggatta ttcatcacag caattatatc cgctggtttg aagaggctcg
     9721 ggtagcctta ttggtacatc tagggtttcc ctataaaaaa atcgaagaat ctgggatcat
     9781 catccccgtt ctggaagtaa attgcagcta taaagaaatg atccattacg gagaaaaagt
     9841 gagcatccat ccaactatcc aaaagtatac ggggacacgg ttggatttta gctacgaaat
     9901 tgtgggaact gaagacaaaa aattaaaaac tactggcagt agtaaacact gttttttact
     9961 ttctgattcg caacgattag tgaagctgag caaagtcaat ccggagttag atcagttatt
    10021 ccaagcgtac gcttctttgg atgatgtaaa ttcttaataa agaccgtcta agcatttttc
    10081 ttgtattgtg tcgagtagtt cgtataatga aaagaaaaaa gcaggtgatc cccatgtatg
    10141 tcgttaaagt aatgcacggt tacatcgata aaactggctg tcgcactcgg gaaaaaaact
    10201 tagacaatct gctcattttc aaagacaaaa aagaatccga agcttttgct aaaagaatcg
    10261 gcggtcgtgt caaaccgatc caagaggtac gaccagattg attcaatatt tttcttttcg
    10321 aggctctaat aggcatgcct atcagggctt tttttgcaga ctgtgttttt atgcccacac
    10381 gatttgtaag cgatttatgt tataatagat tatatctgca tagaaaggaa ggttaaatgg
    10441 ttatcaataa tttgcaacag caagcttatg aatctatacg taaacaaatc atttattccg
    10501 agttagcacc tggaagtaaa atctccgata aagtgttaga agaacaacta gctatcgggc
    10561 gtacaccgat tcgagaagca ctgatccaac tgcgcaatca agagcttgta aaaaccattc
    10621 cacagtccgg cacttatgtt tcaaaaatcg atatccgatc tgcttcatta gcccgttata
    10681 cgcgggaaaa gttggaaaaa ccgatcctgc aagaatgcag tgcgaaaatg accaagcaag
    10741 atcaagctaa actagaaaaa atactagaac aaaccgatca ggcagtaatc gctcaaaata
    10801 aaaaagcttt ttttgatttg gatactgatt ttcaccgaac ttgttatgaa attgctggaa
    10861 aacgagaaat ttgggattgg ctagaaagtt atagtacgcg tttcagatgg ttacgtttga
    10921 cgatttcaga attagactgg ggtcgagtat tagatgaaca tcagacaatg cttcaatcaa
    10981 tgattgatca taactttgat gaagtcggat ttctttgcac gatgcattta catatgatta
    11041 tcgaggagca agaatacgtg attcataatt accccgatta ttttgaagat attcaggaca
    11101 gacccatcaa ataagaggtt ttggaatcag cgttttgacc tgagtattag agaaagaaac
    11161 ggcaaaatag ttcctcatat tttgccgttt tttttgagct aataccgagg gtcagctgat
    11221 tgcacaccgt ttattgagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    11281 caagtaaaaa tagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc
    11341 ttttgaacac cgttttccaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa
    11401 caatttcaaa aaacagcttc tcatgtttgt tgaatatttt gacttgtgac cgaggagttg
    11461 acaggagaac atcgtttatt cggaaaaaaa ggactgtgac acgacttttg tcacagtctc
    11521 tttatttgta atctaaatca tactccgtac atcttttttc tatttttttt atttcttcta
    11581 agatcccttg gctacgaatt tcattatatg gccaaagtaa gactgcctct aatacataag
    11641 gcgaaaaatc gctttgattc atctcttttg aaaaactcat cgctgctatc tcgctcaatg
    11701 cacgaatgtt tgattgatgt gtataaaaag gcaaccgcca cagcacaatt ttttcgcttt
    11761 gcgtataaat tcccttaaca gaagcttcga tgtggtgaaa tagttcatgt ccaataatca
    11821 aagcttgcgc tgtttcttta gagatcgaag ctagtcctga acattttaat ttttctaacg
    11881 gctccgtcat caatcgaatc ccttcatctt ctgcataagt tgcaaaagtg attcgttcgt
    11941 ttatttgctc tgattcacag gaaacgattg gaacagccat ttgttcacaa atttctccta
    12001 catcggcaaa agggtacttt tttttgatgc tacatgccgt ttcgatacca caatcgattg
    12061 attgttgtat aagaattttc tgcttggaca aaggaatctt tttccaaaat aaatctcgcc
    12121 gaaactggta gaatccccaa atttcttcgg ggatctgttg taattcgttt agtaactctt
    12181 ctaacttatg tttgtttgat atttccggca acgacgatca cttcttcctc cggttccata
    12241 gtagatactt ccagatccat caaaagctgt agttgttcca gatccgtcgc tgtaaaatca
    12301 gagactcttg ctcctaagca gagataaaat tctggtcggg caatcaattc agtttgataa
    12361 actgccatat cttcccaaag ttgttcgaca gctgtcatat aattggctgc agttgttttc
    12421 atacatgaag catgtccacg ttggaccttg ataaatcctt tttgatcgat gatccgaaga
    12481 cttccagcat tttttgaacg attctgttcg ccgcatacat aaaagaaagg tgtattttcc
    12541 aataagcaaa cttcattttt atttaatcgc atatcctctg cagcaagctc tagtgcttct
    12601 tctgttgtga tctccttggt tagatcggtt gctttcactt ccgtagaacc agtagcgata
    12661 gccgttactt ttgaagtctg tggatcgatt tctacgtgga cttcgatcga ttctggtgta
    12721 gcgcctgatt caatcgcttt attcatcgct tcatttttca acgatcgtat atcttcttta
    12781 cttggcgaag ggatgatacg ttctacaaca tctcggacca ttgcaagtgc tacaccaata
    12841 gaagaaatca cttcggcgtt ttcaggaatc gaatatttca cacccatttt attactaaag
    12901 taagtaatca atgaggcagc gccgccccct acacctacaa gagaaatctg atctttctcc
    12961 aagtgatact tgtctgccaa ttctaaaatc accggttcaa tcttagcata ggatttttcc
    13021 atgatctgtt ctgcgatgtc ttcaaccgtc gtatgacaat aatcagctaa tgcttggatg
    13081 gcttttcgtg cagatgggac atttccataa gaaaaatgtt cttcttgcac taatccaagg
    13141 acattggctg cacatgtatt tgtaatagtc acttcttctc cgtcttccat cactaccttg
    13201 acgtaatcag cagggtctcc ttctttagga gagaaaaatt caaccttagg acctttgatt
    13261 ttttctgtct ccgtaaatac tgcataatct agaccggcaa tatgtgcaga acgtggaccg
    13321 acatcgatga ttcctgactg attagcccgt accatcgaac cgcctgccac acctagaacc
    13381 cgaacatcta atgaagagat atatgttggg tggccaccta caattgaata atcgattgct
    13441 ggacgtccat ttttaatgac cccgatattg gttgttgttc cgcctacttc aaaataaaca
    13501 ccattagacg ctctcaagta catcaaagag cccatgactg atgctgctgg accggaaagc
    13561 atggttaaaa caggtcgttt tttcatttca ttgatttcca ttactccgcc gtctccgcgc
    13621 ataatcatca aagaaacatt gacccctgct tctcgtacgg aatcttctgt agaggttgcc
    13681 gtatctagca tttttggtaa aatagatgca ttgattgctg ctgtccgagt tcttcttgtc
    13741 aatccatata attttgtgat gtcagaagcc atcgttgtag ggatcgattt taccgatgct
    13801 gcttcataaa caactcgttc tggttcccca ttatctactc caaatgccat agaagaaacc
    13861 aagacctccg ctctttctct ttctaaagaa gaaatcgttt ctgaaacacg gtctacattt
    13921 aagtgtttga caggtaagaa tgcattgaca atctctattt ttttcttatt ccctaaatcg
    13981 atatcgttca atctagtttg tcgttttgct aaaaaacctt ctaatccacc ttttgccatc
    14041 ccgattacgc cgacttttgc tacgtctcct tcaatcagtg cattagtcgc ttgtgtggta
    14101 gaatgtgcaa caaacacaac atcttctgga ctgatattat tttccagcaa acaattttga
    14161 aaagactgaa cgacacctgc ggctactcca cggacatcat cgtgggttgt tttcactgaa
    14221 gattttccga tgatttcgtg cgtcgcatta tcgattgcga ctgctttcgt atgtgtaccg
    14281 ccaacatcaa ttcccattcg cacaaaacgt ttcattttct tttcctccat tcatctaatc
    14341 gtttttatcg acgttttgtt tactaggcgt tcccgtacaa taagaacgtg atcaggcata
    14401 agatcgctcc gatgatccaa ccagttggga tggatttctt catatattct cgagaagtta
    14461 cttttgtata acctaatccc caagcaaccc aagattgtgt aatatctaaa tgctgaggag
    14521 caatcgttgt gattgcaaat aacgggaaca aaaaggctgg cgacatcgtt ggattaacag
    14581 ctaatacgac agctaaaata gctgagcctg aaccgactag attcattgga ccgcggaaaa
    14641 atcctagtgg tgtcaatata gcaaatacga ttgccaagac taatggtgat tgcggtatcc
    14701 aatctccaaa aatagctgaa aaataaggag aagcatacgc agctgaatta ttgaacatcg
    14761 ctaaggtcat caagaaacca accattggcg caacatctac tgcgccatct gtaaataatt
    14821 tcgcaaactg acgacagatt tctttataac ctccgcgcaa ttttccagta aacagcaatg
    14881 cccagatacc tgctagaata aaacctaaga tgattggaag atctaaaaga acgaccccta
    14941 aaactggtaa taatacggct aaccatgaga tcattggtgc gtcgccacct tcagctggta
    15001 cgtttgctgc cattgcatag cgtttctttt tgttcattga tatattagcg accgttagta
    15061 ccacaactaa gcttacaatc atcccaatca taccaatttg gaaatagtca ttataagtat
    15121 agctttcagc cgctggattg aaaccagcat aaatcgtttg atattgtttg aaattgacga
    15181 tattagcaaa gattccagcc ataatcgagc ccataaatga gaacagtgca accggtactg
    15241 aaatacctaa tgacatcaag attggcaaca caataactgc aatagaaatc actggtccga
    15301 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
    15361 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
    15421 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
    15481 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
    15541 tcgccgggtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
    15601 tgatggcaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
    15661 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
    15721 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
    15781 ctttcattaa agggtgacca taaatgttca gttttcaatt aaaaaacctt aattatgcta
    15841 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
    15901 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
    15961 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
    16021 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
    16081 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
    16141 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
    16201 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
    16261 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
    16321 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
    16381 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
    16441 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
    16501 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
    16561 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
    16621 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
    16681 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
    16741 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
    16801 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
    16861 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
    16921 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
    16981 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
    17041 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
    17101 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
    17161 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
    17221 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
    17281 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
    17341 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
    17401 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
    17461 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
    17521 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
    17581 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
    17641 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
    17701 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
    17761 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
    17821 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
    17881 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
    17941 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
    18001 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
    18061 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
    18121 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
    18181 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
    18241 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
    18301 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
    18361 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
    18421 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
    18481 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
    18541 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
    18601 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
    18661 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
    18721 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
    18781 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
    18841 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
    18901 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
    18961 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
    19021 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
    19081 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
    19141 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
    19201 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
    19261 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
    19321 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
    19381 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
    19441 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
    19501 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
    19561 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
    19621 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
    19681 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
    19741 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
    19801 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
    19861 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
    19921 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
    19981 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
    20041 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
    20101 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
    20161 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
    20221 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
    20281 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
    20341 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
    20401 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
    20461 tattagaaaa tgtccagctt tcggaaaaaa tcgcccatgg taatcctagt ggaatcgatg
    20521 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
    20581 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
    20641 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
    20701 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
    20761 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
    20821 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
    20881 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
    20941 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
    21001 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
    21061 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
    21121 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
    21181 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
    21241 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
    21301 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
    21361 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
    21421 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
    21481 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
    21541 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
    21601 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
    21661 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
    21721 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
    21781 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
    21841 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
    21901 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
    21961 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
    22021 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
    22081 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
    22141 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
    22201 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
    22261 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
    22321 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
    22381 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
    22441 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
    22501 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
    22561 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
    22621 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
    22681 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
    22741 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
    22801 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
    22861 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
    22921 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
    22981 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
    23041 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
    23101 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
    23161 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
    23221 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
    23281 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
    23341 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
    23401 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
    23461 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
    23521 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
    23581 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
    23641 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
    23701 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
    23761 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
    23821 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
    23881 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
    23941 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
    24001 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
    24061 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
    24121 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
    24181 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
    24241 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
    24301 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
    24361 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
    24421 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
    24481 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
    24541 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
    24601 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
    24661 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
    24721 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
    24781 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
    24841 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
    24901 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
    24961 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
    25021 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
    25081 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
    25141 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
    25201 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    25261 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    25321 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
    25381 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
    25441 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
    25501 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
    25561 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
    25621 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
    25681 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
    25741 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
    25801 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    25861 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    25921 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    25981 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    26041 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    26101 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    26161 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    26221 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    26281 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    26341 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    26401 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    26461 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    26521 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    26581 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    26641 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    26701 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    26761 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    26821 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    26881 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    26941 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    27001 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    27061 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    27121 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    27181 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    27241 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    27301 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    27361 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    27421 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    27481 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    27541 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    27601 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    27661 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    27721 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    27781 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    27841 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    27901 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    27961 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    28021 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    28081 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    28141 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    28201 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    28261 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    28321 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    28381 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    28441 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    28501 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    28561 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    28621 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    28681 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    28741 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    28801 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    28861 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    28921 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    28981 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    29041 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    29101 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    29161 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    29221 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    29281 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    29341 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    29401 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    29461 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    29521 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    29581 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    29641 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    29701 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    29761 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    29821 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    29881 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    29941 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    30001 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    30061 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    30121 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    30181 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    30241 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    30301 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    30361 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    30421 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    30481 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    30541 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    30601 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    30661 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    30721 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    30781 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    30841 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    30901 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    30961 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    31021 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    31081 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    31141 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    31201 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    31261 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    31321 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    31381 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    31441 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    31501 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    31561 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    31621 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    31681 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    31741 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    31801 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    31861 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    31921 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    31981 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    32041 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    32101 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    32161 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    32221 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    32281 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    32341 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    32401 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    32461 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    32521 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    32581 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    32641 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    32701 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    32761 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    32821 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    32881 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    32941 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    33001 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    33061 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    33121 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    33181 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    33241 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    33301 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    33361 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    33421 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    33481 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    33541 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    33601 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    33661 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    33721 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    33781 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    33841 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    33901 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    33961 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    34021 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    34081 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    34141 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    34201 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    34261 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    34321 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    34381 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    34441 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    34501 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    34561 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    34621 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    34681 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    34741 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    34801 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    34861 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    34921 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    34981 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    35041 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    35101 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    35161 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    35221 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    35281 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    35341 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    35401 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    35461 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    35521 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    35581 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    35641 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    35701 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    35761 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    35821 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    35881 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    35941 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    36001 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    36061 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    36121 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    36181 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    36241 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    36301 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    36361 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    36421 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    36481 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    36541 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    36601 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    36661 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    36721 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    36781 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    36841 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    36901 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    36961 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    37021 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    37081 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    37141 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    37201 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    37261 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    37321 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    37381 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    37441 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    37501 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    37561 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    37621 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    37681 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    37741 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    37801 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    37861 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    37921 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    37981 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    38041 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    38101 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    38161 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    38221 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    38281 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    38341 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    38401 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    38461 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    38521 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    38581 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    38641 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    38701 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    38761 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    38821 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    38881 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    38941 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    39001 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    39061 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    39121 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    39181 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    39241 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    39301 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    39361 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    39421 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    39481 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    39541 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    39601 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    39661 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    39721 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    39781 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    39841 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    39901 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    39961 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    40021 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    40081 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    40141 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    40201 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    40261 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    40321 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    40381 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    40441 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    40501 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    40561 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    40621 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    40681 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    40741 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    40801 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    40861 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    40921 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    40981 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    41041 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    41101 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    41161 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    41221 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    41281 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    41341 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    41401 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    41461 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    41521 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    41581 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    41641 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    41701 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    41761 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    41821 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    41881 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    41941 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    42001 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    42061 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    42121 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    42181 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    42241 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    42301 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    42361 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    42421 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    42481 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    42541 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    42601 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    42661 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    42721 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    42781 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    42841 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    42901 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    42961 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    43021 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    43081 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    43141 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    43201 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    43261 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    43321 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    43381 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    43441 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    43501 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    43561 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    43621 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    43681 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    43741 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    43801 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    43861 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    43921 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    43981 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    44041 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    44101 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    44161 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    44221 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    44281 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    44341 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    44401 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    44461 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    44521 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    44581 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    44641 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    44701 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    44761 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    44821 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    44881 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    44941 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    45001 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    45061 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    45121 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    45181 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    45241 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    45301 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    45361 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    45421 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    45481 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    45541 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    45601 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    45661 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    45721 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    45781 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    45841 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    45901 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    45961 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    46021 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    46081 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    46141 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    46201 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    46261 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    46321 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    46381 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    46441 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    46501 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    46561 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    46621 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    46681 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    46741 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    46801 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    46861 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    46921 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    46981 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    47041 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    47101 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    47161 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    47221 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    47281 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    47341 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    47401 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    47461 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    47521 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    47581 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    47641 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    47701 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    47761 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    47821 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    47881 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    47941 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    48001 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    48061 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    48121 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    48181 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    48241 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    48301 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    48361 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    48421 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    48481 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    48541 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    48601 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    48661 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    48721 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    48781 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    48841 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    48901 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    48961 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    49021 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    49081 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    49141 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    49201 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    49261 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    49321 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    49381 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    49441 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    49501 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    49561 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    49621 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    49681 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    49741 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    49801 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    49861 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    49921 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    49981 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    50041 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    50101 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    50161 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    50221 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    50281 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    50341 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    50401 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    50461 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    50521 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    50581 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    50641 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    50701 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    50761 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    50821 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    50881 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    50941 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    51001 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    51061 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    51121 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    51181 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    51241 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    51301 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    51361 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    51421 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    51481 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    51541 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    51601 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    51661 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    51721 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    51781 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    51841 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    51901 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    51961 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    52021 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    52081 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    52141 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    52201 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    52261 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    52321 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    52381 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    52441 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    52501 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    52561 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    52621 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    52681 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    52741 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    52801 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    52861 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    52921 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    52981 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    53041 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    53101 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    53161 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    53221 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    53281 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    53341 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    53401 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    53461 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    53521 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    53581 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    53641 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    53701 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    53761 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    53821 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    53881 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    53941 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    54001 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    54061 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    54121 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    54181 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    54241 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    54301 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    54361 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    54421 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    54481 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    54541 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    54601 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    54661 cttatgggca aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    54721 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    54781 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    54841 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    54901 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    54961 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    55021 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    55081 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    55141 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    55201 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    55261 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    55321 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    55381 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    55441 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    55501 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    55561 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    55621 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    55681 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    55741 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    55801 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    55861 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    55921 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    55981 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    56041 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    56101 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    56161 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    56221 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    56281 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    56341 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    56401 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    56461 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    56521 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    56581 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    56641 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    56701 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    56761 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    56821 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    56881 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    56941 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    57001 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    57061 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    57121 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    57181 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    57241 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    57301 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    57361 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    57421 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    57481 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    57541 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    57601 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    57661 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    57721 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    57781 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    57841 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    57901 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    57961 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    58021 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    58081 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    58141 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    58201 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    58261 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    58321 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    58381 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    58441 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    58501 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    58561 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    58621 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    58681 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    58741 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    58801 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    58861 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    58921 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    58981 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    59041 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    59101 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    59161 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    59221 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    59281 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    59341 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    59401 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    59461 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    59521 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    59581 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    59641 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    59701 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    59761 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    59821 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    59881 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    59941 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    60001 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    60061 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    60121 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    60181 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    60241 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    60301 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    60361 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    60421 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    60481 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    60541 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    60601 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    60661 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    60721 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    60781 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    60841 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    60901 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    60961 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    61021 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    61081 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    61141 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    61201 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    61261 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    61321 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    61381 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    61441 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    61501 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    61561 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    61621 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    61681 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    61741 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    61801 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    61861 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    61921 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    61981 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    62041 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    62101 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    62161 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    62221 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    62281 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    62341 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    62401 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    62461 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    62521 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    62581 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    62641 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    62701 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    62761 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    62821 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    62881 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    62941 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    63001 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    63061 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    63121 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    63181 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    63241 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    63301 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    63361 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    63421 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    63481 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    63541 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    63601 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    63661 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    63721 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    63781 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    63841 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    63901 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    63961 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    64021 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    64081 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    64141 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    64201 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    64261 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    64321 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    64381 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    64441 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    64501 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    64561 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    64621 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    64681 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    64741 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    64801 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    64861 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    64921 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    64981 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    65041 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    65101 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    65161 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    65221 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    65281 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    65341 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    65401 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    65461 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    65521 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    65581 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    65641 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    65701 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    65761 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    65821 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    65881 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    65941 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    66001 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    66061 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    66121 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    66181 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    66241 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    66301 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    66361 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    66421 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    66481 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    66541 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    66601 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    66661 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    66721 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    66781 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    66841 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    66901 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    66961 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    67021 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    67081 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    67141 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    67201 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    67261 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    67321 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    67381 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    67441 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    67501 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    67561 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    67621 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    67681 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    67741 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    67801 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    67861 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    67921 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    67981 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    68041 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    68101 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    68161 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    68221 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    68281 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    68341 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    68401 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    68461 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    68521 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    68581 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    68641 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    68701 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    68761 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    68821 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    68881 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    68941 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    69001 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    69061 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    69121 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    69181 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    69241 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    69301 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    69361 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    69421 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    69481 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    69541 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    69601 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    69661 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    69721 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    69781 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    69841 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    69901 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    69961 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    70021 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    70081 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    70141 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    70201 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    70261 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    70321 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    70381 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    70441 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    70501 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    70561 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    70621 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    70681 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    70741 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    70801 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    70861 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    70921 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    70981 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    71041 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    71101 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    71161 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    71221 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    71281 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    71341 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    71401 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    71461 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    71521 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    71581 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    71641 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    71701 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    71761 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    71821 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    71881 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    71941 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    72001 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    72061 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    72121 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    72181 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    72241 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    72301 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    72361 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    72421 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    72481 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    72541 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    72601 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    72661 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
    72721 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
    72781 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
    72841 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
    72901 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
    72961 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
    73021 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
    73081 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
    73141 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
    73201 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
    73261 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
    73321 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
    73381 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
    73441 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
    73501 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
    73561 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
    73621 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
    73681 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
    73741 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
    73801 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
    73861 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
    73921 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
    73981 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
    74041 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
    74101 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
    74161 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
    74221 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
    74281 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
    74341 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
    74401 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
    74461 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
    74521 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
    74581 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
    74641 tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg
    74701 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
    74761 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
    74821 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
    74881 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
    74941 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
    75001 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
    75061 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
    75121 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
    75181 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
    75241 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
    75301 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
    75361 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
    75421 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
    75481 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
    75541 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
    75601 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
    75661 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
    75721 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
    75781 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
    75841 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
    75901 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
    75961 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
    76021 actgcctcct
//
LOCUS       DADVXO010000002        75386 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.002, whole genome shotgun sequence.
ACCESSION   DADVXO010000002 DADVXO010000000
VERSION     DADVXO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 75386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.002"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(155..280)
                     /locus_tag="KB950_000073"
     CDS             complement(155..280)
                     /locus_tag="KB950_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6856320.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(414..1307)
                     /locus_tag="KB950_000074"
     CDS             complement(414..1307)
                     /locus_tag="KB950_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBB6856321.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1489..2415)
                     /locus_tag="KB950_000075"
     CDS             complement(1489..2415)
                     /locus_tag="KB950_000075"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBB6856322.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2500..3261)
                     /gene="pflA"
                     /locus_tag="KB950_000076"
     CDS             complement(2500..3261)
                     /gene="pflA"
                     /locus_tag="KB950_000076"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBB6856323.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3505..5736)
                     /gene="pflB"
                     /locus_tag="KB950_000077"
     CDS             complement(3505..5736)
                     /gene="pflB"
                     /locus_tag="KB950_000077"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBB6856324.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6013..8463)
                     /gene="parC"
                     /locus_tag="KB950_000078"
     CDS             complement(6013..8463)
                     /gene="parC"
                     /locus_tag="KB950_000078"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBB6856325.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8475..10520)
                     /gene="parE"
                     /locus_tag="KB950_000079"
     CDS             complement(8475..10520)
                     /gene="parE"
                     /locus_tag="KB950_000079"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBB6856326.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10686..11120)
                     /locus_tag="KB950_000080"
     CDS             complement(10686..11120)
                     /locus_tag="KB950_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBB6856327.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11247..11885
                     /gene="plsY"
                     /locus_tag="KB950_000081"
     CDS             11247..11885
                     /gene="plsY"
                     /locus_tag="KB950_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBB6856328.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12017..12892)
                     /locus_tag="KB950_000082"
     CDS             complement(12017..12892)
                     /locus_tag="KB950_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBB6856329.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(12974..13765)
                     /gene="codY"
                     /locus_tag="KB950_000083"
     CDS             complement(12974..13765)
                     /gene="codY"
                     /locus_tag="KB950_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBB6856330.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13783..15183)
                     /gene="hslU"
                     /locus_tag="KB950_000084"
     CDS             complement(13783..15183)
                     /gene="hslU"
                     /locus_tag="KB950_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBB6856331.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15197..15745)
                     /gene="hslV"
                     /locus_tag="KB950_000085"
     CDS             complement(15197..15745)
                     /gene="hslV"
                     /locus_tag="KB950_000085"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBB6856332.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15782..16687)
                     /gene="xerC"
                     /locus_tag="KB950_000086"
     CDS             complement(15782..16687)
                     /gene="xerC"
                     /locus_tag="KB950_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBB6856333.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16885..18261)
                     /gene="trmFO"
                     /locus_tag="KB950_000087"
     CDS             complement(16885..18261)
                     /gene="trmFO"
                     /locus_tag="KB950_000087"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBB6856334.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18230..20308)
                     /gene="topA"
                     /locus_tag="KB950_000088"
     CDS             complement(18230..20308)
                     /gene="topA"
                     /locus_tag="KB950_000088"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBB6856335.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20430..21287)
                     /gene="dprA"
                     /locus_tag="KB950_000089"
     CDS             complement(20430..21287)
                     /gene="dprA"
                     /locus_tag="KB950_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBB6856336.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21343..22110)
                     /locus_tag="KB950_000090"
     CDS             complement(21343..22110)
                     /locus_tag="KB950_000090"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBB6856337.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22103..22963)
                     /gene="ylqF"
                     /locus_tag="KB950_000091"
     CDS             complement(22103..22963)
                     /gene="ylqF"
                     /locus_tag="KB950_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBB6856338.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23223..23621
                     /locus_tag="KB950_000092"
     CDS             23223..23621
                     /locus_tag="KB950_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6856339.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            23900..24400
                     /locus_tag="KB950_000093"
     CDS             23900..24400
                     /locus_tag="KB950_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBB6856340.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(24483..25034)
                     /gene="lepB"
                     /locus_tag="KB950_000094"
     CDS             complement(24483..25034)
                     /gene="lepB"
                     /locus_tag="KB950_000094"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6856341.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25186..25413)
                     /locus_tag="KB950_000095"
     CDS             complement(25186..25413)
                     /locus_tag="KB950_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBB6856342.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25410..25928)
                     /gene="msrA"
                     /locus_tag="KB950_000096"
     CDS             complement(25410..25928)
                     /gene="msrA"
                     /locus_tag="KB950_000096"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6856343.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(25948..26661)
                     /locus_tag="KB950_000097"
     CDS             complement(25948..26661)
                     /locus_tag="KB950_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBB6856344.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(26664..27560)
                     /locus_tag="KB950_000098"
     CDS             complement(26664..27560)
                     /locus_tag="KB950_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6856345.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(27718..28560)
                     /locus_tag="KB950_000099"
     CDS             complement(27718..28560)
                     /locus_tag="KB950_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6856346.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            28692..29345
                     /locus_tag="KB950_000100"
     CDS             28692..29345
                     /locus_tag="KB950_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBB6856347.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29408..29935)
                     /locus_tag="KB950_000101"
     CDS             complement(29408..29935)
                     /locus_tag="KB950_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6856348.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(29955..30902)
                     /locus_tag="KB950_000102"
     CDS             complement(29955..30902)
                     /locus_tag="KB950_000102"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBB6856349.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(30914..32800)
                     /locus_tag="KB950_000103"
     CDS             complement(30914..32800)
                     /locus_tag="KB950_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6856350.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(32960..35038)
                     /locus_tag="KB950_000104"
     CDS             complement(32960..35038)
                     /locus_tag="KB950_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBB6856351.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35051..35326)
                     /locus_tag="KB950_000105"
     CDS             complement(35051..35326)
                     /locus_tag="KB950_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856352.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(35688..36143)
                     /locus_tag="KB950_000106"
     CDS             complement(35688..36143)
                     /locus_tag="KB950_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856353.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36297..37505)
                     /locus_tag="KB950_000107"
     CDS             complement(36297..37505)
                     /locus_tag="KB950_000107"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBB6856354.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(37617..37949)
                     /locus_tag="KB950_000108"
     CDS             complement(37617..37949)
                     /locus_tag="KB950_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6856355.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38097..38972
                     /locus_tag="KB950_000109"
     CDS             38097..38972
                     /locus_tag="KB950_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6856356.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39054..39635)
                     /locus_tag="KB950_000110"
     CDS             complement(39054..39635)
                     /locus_tag="KB950_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBB6856357.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(39641..40900)
                     /locus_tag="KB950_000111"
     CDS             complement(39641..40900)
                     /locus_tag="KB950_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6856358.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(40902..41948)
                     /locus_tag="KB950_000112"
     CDS             complement(40902..41948)
                     /locus_tag="KB950_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856359.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42183..42458)
                     /locus_tag="KB950_000113"
     CDS             complement(42183..42458)
                     /locus_tag="KB950_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBB6856360.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(42701..44011)
                     /gene="der"
                     /locus_tag="KB950_000114"
     CDS             complement(42701..44011)
                     /gene="der"
                     /locus_tag="KB950_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBB6856361.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44193..45422)
                     /gene="rpsA"
                     /locus_tag="KB950_000115"
     CDS             complement(44193..45422)
                     /gene="rpsA"
                     /locus_tag="KB950_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBB6856362.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(45557..46237)
                     /locus_tag="KB950_000116"
     CDS             complement(45557..46237)
                     /locus_tag="KB950_000116"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBB6856363.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46286..46921)
                     /locus_tag="KB950_000117"
     CDS             complement(46286..46921)
                     /locus_tag="KB950_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6856364.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(46987..48390)
                     /locus_tag="KB950_000118"
     CDS             complement(46987..48390)
                     /locus_tag="KB950_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBB6856365.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(48377..49408)
                     /locus_tag="KB950_000119"
     CDS             complement(48377..49408)
                     /locus_tag="KB950_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6856366.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            49439..49660
                     /locus_tag="KB950_000120"
     CDS             49439..49660
                     /locus_tag="KB950_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBB6856367.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(49825..50562)
                     /locus_tag="KB950_000121"
     CDS             complement(49825..50562)
                     /locus_tag="KB950_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBB6856368.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(50715..52055)
                     /locus_tag="KB950_000122"
     CDS             complement(50715..52055)
                     /locus_tag="KB950_000122"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBB6856369.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52292..53815)
                     /locus_tag="KB950_000123"
     CDS             complement(52292..53815)
                     /locus_tag="KB950_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBB6856370.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54193..54804)
                     /locus_tag="KB950_000124"
     CDS             complement(54193..54804)
                     /locus_tag="KB950_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6856371.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(54927..55052)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55134..55850)
                     /locus_tag="KB950_000125"
     CDS             complement(55134..55850)
                     /locus_tag="KB950_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBB6856372.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(55856..56425)
                     /gene="scpB"
                     /locus_tag="KB950_000126"
     CDS             complement(55856..56425)
                     /gene="scpB"
                     /locus_tag="KB950_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBB6856373.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(56409..57206)
                     /locus_tag="KB950_000127"
     CDS             complement(56409..57206)
                     /locus_tag="KB950_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBB6856374.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57176..57547)
                     /locus_tag="KB950_000128"
     CDS             complement(57176..57547)
                     /locus_tag="KB950_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB6856375.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(57566..58453)
                     /gene="xerD"
                     /locus_tag="KB950_000129"
     CDS             complement(57566..58453)
                     /gene="xerD"
                     /locus_tag="KB950_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBB6856376.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(58549..59022)
                     /locus_tag="KB950_000130"
     CDS             complement(58549..59022)
                     /locus_tag="KB950_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6856377.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59127..59990)
                     /locus_tag="KB950_000131"
     CDS             complement(59127..59990)
                     /locus_tag="KB950_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6856378.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60186..62285)
                     /locus_tag="KB950_000132"
     CDS             complement(60186..62285)
                     /locus_tag="KB950_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBB6856379.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(62505..63614)
                     /gene="rpoD"
                     /locus_tag="KB950_000133"
     CDS             complement(62505..63614)
                     /gene="rpoD"
                     /locus_tag="KB950_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBB6856380.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(63638..65479)
                     /locus_tag="KB950_000134"
     CDS             complement(63638..65479)
                     /locus_tag="KB950_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBB6856381.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(65661..67976)
                     /locus_tag="KB950_000135"
     CDS             complement(65661..67976)
                     /locus_tag="KB950_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6856382.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68161..69021)
                     /locus_tag="KB950_000136"
     CDS             complement(68161..69021)
                     /locus_tag="KB950_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBB6856383.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69080..69601)
                     /locus_tag="KB950_000137"
     CDS             complement(69080..69601)
                     /locus_tag="KB950_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBB6856384.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            69879..70640
                     /locus_tag="KB950_000138"
     CDS             69879..70640
                     /locus_tag="KB950_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6856385.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            70666..73257
                     /gene="mprF"
                     /locus_tag="KB950_000139"
     CDS             70666..73257
                     /gene="mprF"
                     /locus_tag="KB950_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBB6856386.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(73445..74632)
                     /gene="tuf"
                     /locus_tag="KB950_000140"
     CDS             complement(73445..74632)
                     /gene="tuf"
                     /locus_tag="KB950_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBB6856387.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75000..75356
                     /locus_tag="KB950_000141"
     CDS             75000..75356
                     /locus_tag="KB950_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6856388.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20605 a        15901 c        12957 g        25923 t
ORIGIN      
        1 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
       61 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
      121 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
      181 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
      241 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
      301 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
      361 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
      421 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
      481 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
      541 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
      601 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
      661 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
      721 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
      781 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
      841 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
      901 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
      961 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
     1021 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
     1081 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
     1141 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
     1201 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
     1261 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
     1321 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
     1381 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
     1441 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
     1501 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
     1561 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
     1621 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
     1681 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
     1741 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
     1801 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
     1861 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
     1921 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
     1981 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
     2041 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
     2101 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
     2161 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
     2221 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
     2281 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
     2341 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
     2401 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
     2461 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
     2521 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
     2581 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
     2641 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
     2701 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
     2761 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
     2821 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
     2881 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
     2941 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
     3001 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
     3061 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
     3121 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
     3181 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
     3241 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
     3301 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
     3361 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
     3421 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
     3481 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
     3541 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
     3601 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
     3661 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
     3721 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
     3781 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
     3841 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
     3901 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
     3961 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
     4021 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
     4081 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
     4141 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
     4201 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
     4261 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
     4321 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
     4381 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
     4441 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
     4501 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
     4561 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
     4621 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
     4681 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
     4741 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
     4801 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
     4861 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
     4921 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
     4981 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
     5041 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
     5101 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
     5161 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
     5221 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
     5281 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
     5341 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
     5401 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
     5461 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
     5521 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
     5581 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
     5641 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
     5701 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
     5761 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
     5821 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
     5881 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
     5941 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
     6001 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
     6061 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
     6121 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
     6181 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
     6241 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
     6301 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
     6361 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
     6421 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
     6481 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
     6541 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
     6601 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
     6661 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
     6721 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
     6781 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
     6841 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
     6901 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
     6961 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
     7021 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
     7081 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
     7141 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
     7201 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
     7261 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
     7321 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
     7381 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
     7441 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
     7501 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
     7561 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
     7621 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
     7681 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
     7741 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
     7801 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
     7861 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
     7921 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
     7981 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
     8041 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
     8101 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
     8161 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
     8221 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
     8281 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
     8341 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
     8401 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
     8461 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
     8521 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
     8581 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
     8641 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
     8701 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
     8761 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
     8821 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
     8881 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
     8941 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
     9001 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
     9061 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
     9121 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
     9181 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
     9241 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
     9301 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
     9361 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
     9421 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
     9481 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
     9541 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
     9601 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
     9661 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
     9721 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
     9781 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
     9841 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
     9901 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
     9961 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    10021 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    10081 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    10141 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    10201 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    10261 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    10321 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    10381 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    10441 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    10501 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    10561 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    10621 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    10681 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    10741 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    10801 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    10861 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    10921 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    10981 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    11041 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    11101 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    11161 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    11221 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    11281 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    11341 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    11401 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    11461 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    11521 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    11581 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    11641 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    11701 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    11761 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    11821 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    11881 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    11941 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    12001 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    12061 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    12121 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    12181 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    12241 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    12301 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    12361 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    12421 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    12481 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    12541 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    12601 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    12661 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    12721 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    12781 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    12841 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    12901 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    12961 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    13021 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    13081 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    13141 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    13201 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    13261 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    13321 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    13381 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    13441 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    13501 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    13561 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    13621 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    13681 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    13741 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    13801 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    13861 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    13921 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    13981 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    14041 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    14101 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    14161 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    14221 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    14281 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    14341 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    14401 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    14461 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    14521 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    14581 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    14641 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    14701 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    14761 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    14821 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    14881 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    14941 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    15001 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    15061 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    15121 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    15181 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    15241 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    15301 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    15361 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    15421 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    15481 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    15541 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    15601 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    15661 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    15721 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    15781 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    15841 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    15901 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    15961 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    16021 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    16081 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    16141 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    16201 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    16261 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    16321 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    16381 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    16441 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    16501 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    16561 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    16621 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    16681 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    16741 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    16801 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    16861 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    16921 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    16981 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    17041 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    17101 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    17161 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    17221 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    17281 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    17341 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    17401 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    17461 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    17521 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    17581 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    17641 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    17701 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    17761 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    17821 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    17881 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    17941 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    18001 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    18061 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    18121 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    18181 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    18241 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    18301 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    18361 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    18421 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    18481 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    18541 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    18601 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    18661 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    18721 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    18781 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    18841 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    18901 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    18961 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    19021 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    19081 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    19141 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    19201 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    19261 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    19321 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    19381 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    19441 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    19501 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    19561 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    19621 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    19681 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    19741 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    19801 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    19861 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    19921 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    19981 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    20041 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    20101 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    20161 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    20221 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    20281 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    20341 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    20401 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    20461 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    20521 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    20581 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    20641 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    20701 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    20761 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    20821 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    20881 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    20941 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    21001 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    21061 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    21121 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    21181 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    21241 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    21301 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    21361 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    21421 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    21481 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    21541 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    21601 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    21661 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    21721 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    21781 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    21841 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    21901 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    21961 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    22021 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    22081 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    22141 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    22201 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    22261 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    22321 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    22381 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    22441 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    22501 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    22561 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    22621 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    22681 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    22741 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    22801 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    22861 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    22921 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    22981 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    23041 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    23101 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    23161 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    23221 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    23281 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    23341 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    23401 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    23461 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    23521 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    23581 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    23641 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    23701 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    23761 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    23821 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    23881 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    23941 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    24001 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    24061 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    24121 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    24181 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    24241 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    24301 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    24361 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    24421 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    24481 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    24541 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    24601 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    24661 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    24721 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    24781 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    24841 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    24901 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    24961 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    25021 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    25081 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    25141 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    25201 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    25261 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    25321 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    25381 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    25441 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    25501 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    25561 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    25621 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    25681 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    25741 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    25801 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    25861 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    25921 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    25981 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    26041 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    26101 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    26161 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    26221 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    26281 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    26341 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    26401 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    26461 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    26521 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    26581 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    26641 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    26701 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    26761 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    26821 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    26881 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    26941 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    27001 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    27061 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    27121 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    27181 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    27241 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    27301 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    27361 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    27421 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    27481 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    27541 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    27601 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    27661 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    27721 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    27781 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    27841 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    27901 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    27961 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    28021 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    28081 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    28141 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    28201 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    28261 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    28321 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    28381 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    28441 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    28501 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    28561 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    28621 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    28681 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    28741 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    28801 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    28861 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    28921 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    28981 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    29041 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    29101 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    29161 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    29221 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    29281 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    29341 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    29401 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    29461 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    29521 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    29581 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    29641 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    29701 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    29761 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    29821 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    29881 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    29941 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    30001 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    30061 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    30121 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    30181 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    30241 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    30301 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    30361 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    30421 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    30481 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    30541 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    30601 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    30661 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    30721 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    30781 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    30841 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    30901 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    30961 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    31021 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    31081 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    31141 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    31201 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    31261 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    31321 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    31381 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    31441 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    31501 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    31561 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    31621 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    31681 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    31741 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    31801 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    31861 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    31921 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    31981 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    32041 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    32101 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    32161 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    32221 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    32281 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    32341 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    32401 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    32461 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    32521 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    32581 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    32641 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    32701 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    32761 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    32821 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    32881 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    32941 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    33001 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    33061 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    33121 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    33181 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    33241 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    33301 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    33361 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    33421 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    33481 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    33541 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    33601 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    33661 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    33721 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    33781 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    33841 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    33901 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    33961 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    34021 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    34081 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    34141 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    34201 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    34261 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    34321 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    34381 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    34441 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    34501 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    34561 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    34621 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    34681 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    34741 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    34801 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    34861 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    34921 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    34981 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    35041 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    35101 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    35161 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    35221 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    35281 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    35341 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    35401 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    35461 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    35521 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    35581 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    35641 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    35701 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    35761 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    35821 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    35881 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    35941 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    36001 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    36061 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    36121 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    36181 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    36241 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    36301 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    36361 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    36421 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    36481 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    36541 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    36601 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    36661 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    36721 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    36781 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    36841 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    36901 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    36961 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    37021 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    37081 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    37141 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    37201 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    37261 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    37321 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    37381 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    37441 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    37501 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    37561 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    37621 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    37681 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    37741 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    37801 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    37861 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    37921 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    37981 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    38041 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    38101 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    38161 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    38221 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    38281 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    38341 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    38401 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    38461 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    38521 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    38581 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    38641 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    38701 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    38761 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    38821 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    38881 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    38941 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    39001 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    39061 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    39121 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    39181 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    39241 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    39301 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    39361 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    39421 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    39481 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    39541 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    39601 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    39661 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    39721 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    39781 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    39841 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    39901 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    39961 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    40021 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    40081 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    40141 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    40201 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    40261 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    40321 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    40381 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    40441 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    40501 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    40561 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    40621 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    40681 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    40741 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    40801 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    40861 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    40921 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    40981 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    41041 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    41101 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    41161 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    41221 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    41281 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    41341 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    41401 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    41461 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    41521 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    41581 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    41641 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    41701 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    41761 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    41821 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    41881 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    41941 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    42001 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    42061 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    42121 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    42181 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    42241 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    42301 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    42361 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    42421 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    42481 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    42541 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    42601 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    42661 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    42721 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    42781 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    42841 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    42901 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    42961 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    43021 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    43081 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    43141 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    43201 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    43261 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    43321 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    43381 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    43441 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    43501 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    43561 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    43621 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    43681 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    43741 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    43801 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    43861 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    43921 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    43981 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    44041 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    44101 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    44161 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    44221 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    44281 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    44341 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    44401 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    44461 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    44521 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    44581 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    44641 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    44701 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    44761 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    44821 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    44881 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    44941 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    45001 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    45061 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    45121 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    45181 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    45241 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    45301 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    45361 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    45421 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    45481 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    45541 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    45601 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    45661 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    45721 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    45781 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    45841 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    45901 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    45961 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    46021 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    46081 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    46141 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    46201 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    46261 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    46321 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    46381 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    46441 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    46501 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    46561 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    46621 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    46681 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    46741 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    46801 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    46861 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    46921 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    46981 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    47041 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    47101 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    47161 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    47221 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    47281 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    47341 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    47401 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    47461 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    47521 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    47581 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    47641 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    47701 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    47761 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    47821 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    47881 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    47941 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    48001 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    48061 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    48121 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    48181 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    48241 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    48301 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    48361 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    48421 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    48481 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    48541 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    48601 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    48661 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    48721 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    48781 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    48841 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    48901 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    48961 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    49021 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    49081 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    49141 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    49201 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    49261 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    49321 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    49381 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    49441 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    49501 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    49561 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    49621 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    49681 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    49741 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    49801 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    49861 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    49921 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    49981 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    50041 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    50101 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    50161 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    50221 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    50281 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    50341 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    50401 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    50461 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    50521 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    50581 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    50641 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    50701 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    50761 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    50821 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    50881 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    50941 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    51001 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    51061 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    51121 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    51181 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    51241 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    51301 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    51361 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    51421 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    51481 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    51541 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    51601 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    51661 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    51721 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    51781 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    51841 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    51901 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    51961 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    52021 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    52081 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    52141 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    52201 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    52261 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    52321 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    52381 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    52441 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    52501 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    52561 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    52621 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    52681 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    52741 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    52801 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    52861 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    52921 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    52981 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    53041 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    53101 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    53161 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    53221 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    53281 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    53341 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    53401 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    53461 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    53521 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    53581 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    53641 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    53701 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    53761 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    53821 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    53881 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    53941 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    54001 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    54061 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    54121 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    54181 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    54241 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    54301 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    54361 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    54421 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    54481 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    54541 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    54601 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    54661 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    54721 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    54781 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    54841 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    54901 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    54961 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    55021 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    55081 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    55141 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    55201 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    55261 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    55321 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    55381 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    55441 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    55501 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    55561 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    55621 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    55681 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    55741 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    55801 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    55861 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    55921 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    55981 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    56041 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    56101 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    56161 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    56221 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    56281 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    56341 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    56401 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    56461 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    56521 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    56581 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    56641 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    56701 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    56761 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    56821 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    56881 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    56941 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    57001 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    57061 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    57121 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    57181 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    57241 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    57301 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    57361 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    57421 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    57481 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    57541 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    57601 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    57661 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    57721 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    57781 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    57841 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    57901 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    57961 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    58021 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    58081 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    58141 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    58201 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    58261 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    58321 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    58381 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    58441 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    58501 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    58561 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    58621 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    58681 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    58741 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    58801 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    58861 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    58921 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    58981 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    59041 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    59101 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    59161 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    59221 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    59281 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    59341 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    59401 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    59461 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    59521 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    59581 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    59641 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    59701 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    59761 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    59821 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    59881 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    59941 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    60001 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    60061 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    60121 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    60181 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    60241 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    60301 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    60361 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    60421 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    60481 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    60541 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    60601 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    60661 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    60721 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    60781 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    60841 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    60901 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    60961 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    61021 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    61081 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    61141 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    61201 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    61261 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    61321 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    61381 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    61441 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    61501 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    61561 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    61621 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    61681 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    61741 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    61801 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    61861 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    61921 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    61981 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    62041 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    62101 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    62161 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    62221 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    62281 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    62341 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    62401 ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa
    62461 accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct
    62521 tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga
    62581 cgaatacgtt cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg
    62641 ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct
    62701 agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt
    62761 gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt
    62821 tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga
    62881 tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga
    62941 cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta
    63001 cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt
    63061 gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt
    63121 ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt
    63181 actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg
    63241 attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct
    63301 ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct
    63361 gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca
    63421 ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca
    63481 tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt
    63541 cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct
    63601 gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt
    63661 tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc
    63721 tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga
    63781 tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt
    63841 cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa
    63901 ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg
    63961 catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa
    64021 atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc
    64081 gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt
    64141 gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa
    64201 actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa
    64261 gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc
    64321 cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt
    64381 cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa
    64441 cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg
    64501 ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt
    64561 ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga
    64621 taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt
    64681 ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca
    64741 gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg
    64801 caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca
    64861 ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc
    64921 gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct
    64981 cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt
    65041 gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt
    65101 ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag
    65161 ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta
    65221 ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc
    65281 ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg
    65341 tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga
    65401 catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt
    65461 gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca
    65521 gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt
    65581 aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca
    65641 catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca
    65701 gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct
    65761 acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca
    65821 tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa
    65881 gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt
    65941 tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact
    66001 acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt
    66061 tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca
    66121 atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc
    66181 gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg
    66241 ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac
    66301 aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa
    66361 tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg
    66421 ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa
    66481 accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg
    66541 acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga
    66601 ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct
    66661 tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct
    66721 ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca
    66781 ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc
    66841 gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa
    66901 gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata
    66961 gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg
    67021 atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca
    67081 gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa
    67141 tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca
    67201 atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca
    67261 ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc
    67321 cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt
    67381 tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct
    67441 gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc
    67501 aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt
    67561 gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt
    67621 tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg
    67681 atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt
    67741 atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg
    67801 gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac
    67861 gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca
    67921 ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc
    67981 tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc
    68041 aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa
    68101 aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct
    68161 ttacttatat tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa
    68221 aagaacagga caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat
    68281 cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt
    68341 tgagaaaatg taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc
    68401 gatcggcaca atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa
    68461 tcccatatac accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat
    68521 caatccgccg gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg
    68581 agcgccgtat aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc
    68641 agaagtatct cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat
    68701 acatccgaca aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa
    68761 aaaagaaagt agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac
    68821 attttctttg aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc
    68881 aaagacagct gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa
    68941 cattcccttc acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc
    69001 cattttttgc cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga
    69061 ttgaacattt cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat
    69121 tcattttgac aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat
    69181 tgtaaagcaa ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa
    69241 gtgggctcag cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat
    69301 gattttttac ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata
    69361 aatcgcatcc ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat
    69421 caatactgtc agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct
    69481 gcatgagatc tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct
    69541 cgtagttttc catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca
    69601 ctcgttttac cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca
    69661 gaatgatcat gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac
    69721 ctagttattt tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc
    69781 cttgatttct gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac
    69841 gaaaatgatc gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac
    69901 tttacacagt aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga
    69961 gcgaaattta ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg
    70021 acggcagcgg ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt
    70081 atctagtaga tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc
    70141 agttaatggc cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt
    70201 ttcttggatt tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa
    70261 aagttcatcg attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg
    70321 ccagagtcat cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa
    70381 gtcttcgcaa aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa
    70441 atgatttaaa aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca
    70501 agctaaaaca ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga
    70561 aagaacaagg acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc
    70621 aatttctttc tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc
    70681 atacaatggc tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt
    70741 gtcattattg tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga
    70801 gaaacgtttg ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc
    70861 tctgtgttac ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag
    70921 aatcctcgtt acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt
    70981 ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc
    71041 agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt
    71101 tacagtctgc tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa
    71161 tattggatat ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact
    71221 ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta
    71281 gtttcattgt tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg
    71341 gaaatccgga ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga
    71401 atcgtgtcta tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg
    71461 tccgctattg gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc
    71521 tactacatca ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact
    71581 tttgatcagc gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc
    71641 gtggttgtct tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa
    71701 gcttttacag agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc
    71761 caatttccta gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca
    71821 cgtgtgaaac gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt
    71881 ttattaagtg attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt
    71941 cttgcttcta aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg
    72001 atcgatggaa tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat
    72061 ctgccggatt tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa
    72121 aagatttggt tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt
    72181 gttcatttcc ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc
    72241 ctgaagatcc ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat
    72301 aagcggatgt ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc
    72361 ttcaataata aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa
    72421 gcaatcgaag cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa
    72481 acaagcgaag agattgttat gattttgcat gaattcggct acgatttcat taagatgggg
    72541 gaagaagctt atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact
    72601 cgtgctgtct tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca
    72661 ttttctactg agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga
    72721 aaggaaaaag ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt
    72781 gcagttgtca aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac
    72841 acgaacaata aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct
    72901 ggcagtatgg atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa
    72961 tggttcaatt tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta
    73021 caggaaagga ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga
    73081 ttaagagaat ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct
    73141 agagatagtt ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt
    73201 gagcagacac aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa
    73261 ctgcataccg atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag
    73321 ttcatgcttc tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa
    73381 tcctgtttgg accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg
    73441 tttcttatgc ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa
    73501 ttgagaatgt cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca
    73561 cgttgtcacc aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat
    73621 cagttgtgcg gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac
    73681 cttcttcttt tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac
    73741 ctggtttagc aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca
    73801 cgcctacgtt atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg
    73861 ttacaactgc tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac
    73921 gaacagcacc acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga
    73981 cgtcttccac tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt
    74041 catctactgt atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg
    74101 cttttaatgc ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca
    74161 ataactcgcg aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa
    74221 ctttgttcaa gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt
    74281 cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt
    74341 gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg
    74401 cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt
    74461 cacgttcttc tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt
    74521 tgcctaatac tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa
    74581 tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca
    74641 aacctcctaa ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct
    74701 ttttttgact aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc
    74761 ttattatacg tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa
    74821 aaaagtacct tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt
    74881 aactttcttt tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca
    74941 acaactttca atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca
    75001 tgaaaaaatt aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag
    75061 cttatcatta tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa
    75121 tgtcagctac ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg
    75181 cttataataa agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat
    75241 taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg
    75301 aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa
    75361 tggtcttaaa attttattag ataaaa
//
LOCUS       DADVXO010000003        54824 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.003, whole genome shotgun sequence.
ACCESSION   DADVXO010000003 DADVXO010000000
VERSION     DADVXO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 54824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.003"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            84..764
                     /locus_tag="KB950_000142"
     CDS             84..764
                     /locus_tag="KB950_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6856389.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            854..1888
                     /locus_tag="KB950_000143"
     CDS             854..1888
                     /locus_tag="KB950_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBB6856390.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1902..2237
                     /locus_tag="KB950_000144"
     CDS             1902..2237
                     /locus_tag="KB950_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856391.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2221..4701
                     /locus_tag="KB950_000145"
     CDS             2221..4701
                     /locus_tag="KB950_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856392.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            4757..6316
                     /locus_tag="KB950_000146"
     CDS             4757..6316
                     /locus_tag="KB950_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6856393.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6313..7677
                     /locus_tag="KB950_000147"
     CDS             6313..7677
                     /locus_tag="KB950_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6856394.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7848..8918
                     /locus_tag="KB950_000148"
     CDS             7848..8918
                     /locus_tag="KB950_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856395.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8933..9601
                     /locus_tag="KB950_000149"
     CDS             8933..9601
                     /locus_tag="KB950_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6856396.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9621..10199
                     /locus_tag="KB950_000150"
     CDS             9621..10199
                     /locus_tag="KB950_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6856397.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10600..11511
                     /locus_tag="KB950_000151"
     CDS             10600..11511
                     /locus_tag="KB950_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6856398.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11599..12516)
                     /locus_tag="KB950_000152"
     CDS             complement(11599..12516)
                     /locus_tag="KB950_000152"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBB6856399.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12718..13155
                     /gene="zapA"
                     /locus_tag="KB950_000153"
     CDS             12718..13155
                     /gene="zapA"
                     /locus_tag="KB950_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBB6856400.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13214..13762
                     /locus_tag="KB950_000154"
     CDS             13214..13762
                     /locus_tag="KB950_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBB6856401.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            13877..16237
                     /locus_tag="KB950_000155"
     CDS             13877..16237
                     /locus_tag="KB950_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBB6856402.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16324..16638
                     /gene="trxA"
                     /locus_tag="KB950_000156"
     CDS             16324..16638
                     /gene="trxA"
                     /locus_tag="KB950_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBB6856403.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            16820..18598
                     /gene="uvrC"
                     /locus_tag="KB950_000157"
     CDS             16820..18598
                     /gene="uvrC"
                     /locus_tag="KB950_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBB6856404.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18743..19642
                     /locus_tag="KB950_000158"
     CDS             18743..19642
                     /locus_tag="KB950_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6856405.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19635..20897
                     /locus_tag="KB950_000159"
     CDS             19635..20897
                     /locus_tag="KB950_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856406.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21058..22956
                     /locus_tag="KB950_000160"
     CDS             21058..22956
                     /locus_tag="KB950_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBB6856407.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23286..24005)
                     /locus_tag="KB950_000161"
     CDS             complement(23286..24005)
                     /locus_tag="KB950_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBB6856408.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24342..24674
                     /locus_tag="KB950_000162"
     CDS             24342..24674
                     /locus_tag="KB950_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856409.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24661..26664
                     /locus_tag="KB950_000163"
     CDS             24661..26664
                     /locus_tag="KB950_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBB6856410.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26683..27153
                     /locus_tag="KB950_000164"
     CDS             26683..27153
                     /locus_tag="KB950_000164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBB6856411.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27174..27758
                     /locus_tag="KB950_000165"
     CDS             27174..27758
                     /locus_tag="KB950_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBB6856412.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27773..28759
                     /locus_tag="KB950_000166"
     CDS             27773..28759
                     /locus_tag="KB950_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBB6856413.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            28749..29060
                     /locus_tag="KB950_000167"
     CDS             28749..29060
                     /locus_tag="KB950_000167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBB6856414.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29082..30863
                     /locus_tag="KB950_000168"
     CDS             29082..30863
                     /locus_tag="KB950_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBB6856415.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            30856..32232
                     /locus_tag="KB950_000169"
     CDS             30856..32232
                     /locus_tag="KB950_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBB6856416.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32239..32874
                     /locus_tag="KB950_000170"
     CDS             32239..32874
                     /locus_tag="KB950_000170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBB6856417.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            32904..34259
                     /locus_tag="KB950_000171"
     CDS             32904..34259
                     /locus_tag="KB950_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBB6856418.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34451..35845)
                     /locus_tag="KB950_000172"
     CDS             complement(34451..35845)
                     /locus_tag="KB950_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBB6856419.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            35937..36908
                     /locus_tag="KB950_000173"
     CDS             35937..36908
                     /locus_tag="KB950_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBB6856420.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            36905..37687
                     /locus_tag="KB950_000174"
     CDS             36905..37687
                     /locus_tag="KB950_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBB6856421.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(37856..38227)
                     /locus_tag="KB950_000175"
     CDS             complement(37856..38227)
                     /locus_tag="KB950_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6856422.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38361..38804
                     /locus_tag="KB950_000176"
     CDS             38361..38804
                     /locus_tag="KB950_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBB6856423.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(38847..39656)
                     /gene="yidA"
                     /locus_tag="KB950_000177"
     CDS             complement(38847..39656)
                     /gene="yidA"
                     /locus_tag="KB950_000177"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBB6856424.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNNEHGVAEAIKKYVL"
     gene            complement(39675..41042)
                     /locus_tag="KB950_000178"
     CDS             complement(39675..41042)
                     /locus_tag="KB950_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6856425.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41026..41856)
                     /locus_tag="KB950_000179"
     CDS             complement(41026..41856)
                     /locus_tag="KB950_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBB6856426.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42019..42456
                     /locus_tag="KB950_000180"
     CDS             42019..42456
                     /locus_tag="KB950_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBB6856427.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42532..43152
                     /locus_tag="KB950_000181"
     CDS             42532..43152
                     /locus_tag="KB950_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBB6856428.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43341..43847
                     /locus_tag="KB950_000182"
     CDS             43341..43847
                     /locus_tag="KB950_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBB6856429.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            43864..44763
                     /locus_tag="KB950_000183"
     CDS             43864..44763
                     /locus_tag="KB950_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBB6856430.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            44850..45734
                     /locus_tag="KB950_000184"
     CDS             44850..45734
                     /locus_tag="KB950_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB6856431.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(45926..45997)
                     /locus_tag="KB950_000185"
     tRNA            complement(45926..45997)
                     /locus_tag="KB950_000185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45962..45964),aa:Arg,seq:ccg)
     gene            46057..47391
                     /gene="rpoN"
                     /locus_tag="KB950_000186"
     CDS             46057..47391
                     /gene="rpoN"
                     /locus_tag="KB950_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBB6856432.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47540..48631
                     /locus_tag="KB950_000187"
     CDS             47540..48631
                     /locus_tag="KB950_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBB6856433.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            48672..49673
                     /gene="gap"
                     /locus_tag="KB950_000188"
     CDS             48672..49673
                     /gene="gap"
                     /locus_tag="KB950_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB6856434.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            49783..50973
                     /locus_tag="KB950_000189"
     CDS             49783..50973
                     /locus_tag="KB950_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBB6856435.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51077..51832
                     /locus_tag="KB950_000190"
     CDS             51077..51832
                     /locus_tag="KB950_000190"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBB6856436.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            51948..53246
                     /gene="eno"
                     /locus_tag="KB950_000191"
     CDS             51948..53246
                     /gene="eno"
                     /locus_tag="KB950_000191"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBB6856437.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            53645..54784
                     /locus_tag="KB950_000192"
     CDS             53645..54784
                     /locus_tag="KB950_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBB6856438.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        18210 a         9566 c        11596 g        15452 t
ORIGIN      
        1 caagtaagcg gatgaattca tgaggatttt ttgcatactg tctctactat acttaggtca
       61 ctaagaaaaa ggagtgattt attatgaaaa agaacatgat tgggacatta ttactttcaa
      121 cacttcttct aggcggcttg gcaacaccag cttttgctga gggtcaagcg acttcaaaag
      181 gagatattac ctttactgaa ccaacaaata ccgtggagcc tcttaaccct actgatcctt
      241 caaaaccagt agagccagca gatccggaaa atccagcaac tggccagaca ggtcctttaa
      301 cactggatgt agttcccgaa ttgccatttg gtacacacga gattgaaagc ggaacaaaaa
      361 cgtatcaagt agacgcttca aaaaacgata caccttatct ccaagtatca gatcgtcgag
      421 gtgtaggtgc agatggccaa gcacaagggt ggaacgtgac agtttcggtt tcagattttg
      481 taaacggttc acaagttttg caaggcgctg aattagattt tggaacatcc actgttaaaa
      541 gtacgtcaga taacgaatcc actggaccaa ctagtcaaac tgtaactggg ttgagtaaag
      601 caagtgcagc gacaccaata tttacagcag caaaagatca aggtctggga acatggcttt
      661 ctgtttatga tccagcaaat atcacactga aagtgccgaa agcagcagct ggaacattca
      721 cggctgattt gacttggaac ctcgtggctg gcccagtagc atagagtatg aaataaattc
      781 aacaaactgc ggtgaaaata ctataggtga aggatttttt accgcagttt ttgttgaaag
      841 gatgtggtgg acaatgaatc aaagtaaacg actgtttgtt tcgttttttt ctattttaag
      901 tctatttttc atttttccgt caatcagcca agcaaaggat tcagcaggag actttggaat
      961 caaaccggta tttccagaga accaaataga taaagcaatc ggttattttg atttattagt
     1021 ggcaccggaa caaaatcaga cactagaagt aattatcagc aattcttcag atgaagaacg
     1081 aacatttgag gtctctgtca atccggcggt aacgagtgat ggtggaacga tcgattatag
     1141 tcaaaaaaac ccaacgttag atgagacatt gccgtttgat gtccgtgacg ttttgctcat
     1201 tgcgaaaaag gaaatcaatg tatcagcaca tgcagaaaca actgtaccta tagaagtgaa
     1261 aatcccagca aaaagcttta agggacgtgt attagcgggt attcatgttt cacctaaaga
     1321 agaagctgaa acagaaaatg ctacagaagg agcacaaatc aaaaatcgta ttgcttataa
     1381 tctcgcagtc gttttacaag aaagtcaaga gacgatagaa ccggatctga aacttttatc
     1441 aggagatttg gacgaggtca acgcaaagcc aactgttcag cttcgtttcc aaaatcctca
     1501 gcctaggatt atcagcaatt tgatatttac gagcaagatc ttttatgaaa atcagcttta
     1561 tattgaaaat acgtctaatg catttttagt tgcaccaaat tcgaattttc acttgaatct
     1621 tgatttagct ggtgataaag caaaagccgg cgattaccgc gcagaaatca tagcaaaatc
     1681 aggcgacagt aatgaatggc gatttacaca aaactttacg atcaaaaaag aaaaagcaca
     1741 aaaagtaaat gaaaattccg tttttgcagt acaagaacaa tctttcccat ggctttatgc
     1801 aggattggtt gctgcagttc tagtgttatg gttgattttg ctgtttttgt gggtccacaa
     1861 gaaaaagaaa aaaggagaaa caacatgatg aggtgatttc gttgaaaaca tcgatcttat
     1921 tgggagggat tattgcaagt ttgctgttct tcagctggca agagccagtc caagcagata
     1981 ccaaaagttc aggtacgatt acattttatg atctaagtcc aattccgcat ccaagtggtt
     2041 cagtcaatgg cgcatcaaaa cagcagacac agctaccgat ttataaagaa tctaatcaac
     2101 atggcgtatt cccggagacg aatgaacaaa agatccgttt tctttatttg atcattggta
     2161 tagaattatg gcttcttgcg tggttgttgt tatggagaag aaaggaaaag gaggatgaac
     2221 atgttgctac aaaataaatt ttcatttttt cgctccttct tttttacttc gctttttgtt
     2281 ttaagtatat ttatcttttc accttcggca gaagctgttg gagcaacgcc tcctgccatt
     2341 tcttatgatc catacacgta tgaaaatatc gtacctccaa atggtggacg tagtgcagtc
     2401 aatggcggga agcttgtcca actaactggg gctaaccaat tgcctaaatt gactgctact
     2461 tatttgaacg atggttcaaa tgatccttgg aaaggtggca cggtccgttt tcattgggaa
     2521 agccatgtga aactaacaaa tgtacttgat ttggtgaata tcactgcaca aggatttgca
     2581 tttgtggcga tcatcaattt gccggaacat gtatcaagca gtgatgtatt aaaagcaatt
     2641 gactggaaca acgcctactt gtatattgcc ggtgccccca tcaaggttac tagcggaagt
     2701 ttattatcag caggaagcca taccttgcgt tttgggatgg ggtcgtggga tacaaatact
     2761 ctattgacag atattattgc tattattacg cgaggcgaat tcaacgtgga cgatatcccg
     2821 cttcaatttg atgttacagc taatgtcgcg aagatgacag aaaacggaga taaatatgat
     2881 acgagtccaa acaaaatttt gaccaagggt aaattacaac cttctatgtc taaaaaggcc
     2941 gttttttctg ttgattttta tgattcgaat aatattatcc aagatcgtat gttcaattcg
     3001 attttagctc cagctgcagg tagattttat cctaaattaa ctggcggaaa cttgaatcca
     3061 ggagcatatg cattttctgc aagtgcagag ataaatacgt ggaatagtta catctcacca
     3121 agtgattcaa caggaaaata taatacattt gctgatacca cagaatacgt ggatggaaat
     3181 aaccagaacc ttccaggctc taccaatagt cggacattga cgatggggct gactgaagga
     3241 cgttttgatc agttgccatt tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa
     3301 aacgttacac aaggagcaat actgactcat actccagcaa gcacaagtgg attgaatcag
     3361 acagccaata tcgtgtacag tgggaaagat gcgcaaggcg gattgctttc tcctgttgct
     3421 ttaaaggtat tggagcagta caagctagac ggaacggttt taccaacaaa cttaacagcc
     3481 gatagtcagt ggggaaagct cctttcttct gcatcgtacc aagtcacggc caattggtca
     3541 gcaactgaac taaaaaaggg ttcgatccat tacaggcttt attcgaaagg aacccaaaaa
     3601 ttagcgacag gatggacaga tcaacttttc caaacgatcc aatcaaataa cgggggtgta
     3661 aatacagcta cttctacact acctgcattg gcttcagggc aatattattt tgattatcgc
     3721 ttggtggacg atgtacttac taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa
     3781 caagctggaa tcaccactct tcctcaaata acagtggcta attttccatc tgtgagtgca
     3841 gatagtcagc taaaaaacct tagcagacaa cctgagacag gagatcccct gtctgctcta
     3901 tcaggagata cgattcaaga aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata
     3961 accgacaaaa aaatcacgat ctcacttcct gaaaatacta cttatatcaa tggttcctta
     4021 aaattaaatg gtacaactat cgcagatacc ggcattcaac aaggagtttc tgtcccagca
     4081 gatctattga gcaaaatagg agataccatt catcttactt acaactatca attaaatact
     4141 gtagatactt ctttacaatc cgtcagcata ctgacaaagg cagctgtact ttccagcaat
     4201 attacattag cagatggtgc taagcttcct aacccagttg tccaaacatc tgctaaaaca
     4261 attcttgtcc ccaaacaaga attaacatta gttaatgtac cagatgattt tacatttggt
     4321 aacgatctcc ccaaaccgtt gaagacaagt tattatgaat caaaaggcga tttttctttt
     4381 gatgtgcggg atactcggct tccaagcacg tctccttggc aactgacagg tacattgact
     4441 tctttattta agaatgatca aggtcaagaa ttaagcggaa caaaattata cttcaatcat
     4501 tctggatcaa agcaattgat tcagcaaggt caaaacacgt tgatttatga gagtgatgga
     4561 acagctaaag gagaggttct cgttgacttt cccgataccg atggtttact cttagaggta
     4621 aatagcagta cgaatgctca acctggcgca acgtatcaag gcatggtcac atggaaactg
     4681 acagctgggc caaccagcta agtgattaga atttgtatgg atctgtgata actgtcacag
     4741 atcctctttt tcaaggatga gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg
     4801 agtggacttg gaacttttca gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt
     4861 ccaactttta ttagaggaaa aaggagaggt ttccgttaat aacgtactta cccgcttatc
     4921 agttgatcgg gctaccttga aagaagatct gttttattta caagctaact tggaaaatat
     4981 caaacaattg gaaattgtta tccaagaagg gaatatttta ttaatcagac atggaaatat
     5041 agcagcagtc gatgtttatt atcattgtta cttgcgctat tctacaaaat atcagatact
     5101 tctttatctg ttggaaaatg gctcatttga tcggcagaaa cttgtcatgg aacttaatat
     5161 cagttcggct actttgtctc gcagagtgaa ggaattaaat gattctttaa atgagtttca
     5221 tctgaaaata aaaagcgggc ggctgaccgg tccagaaagt caaattcgtt atttttattt
     5281 ccaattattt tggtttggac gtccctatct tgaaaacaaa aaagaataca gtgatttatc
     5341 aggaaacact tttttagatc tgctgcaaaa agagtttagt tttgctatta cagaagaagg
     5401 acgattgaaa tttgaaatat ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa
     5461 atccaaacca gattatagca atcttcctgt tggattgttt caaacgacac aatttttatt
     5521 ggacctccat acatttttga cccgttactt ctttcaatat gcctttcctt ggaatcaaaa
     5581 tgaaacgttg gttttttatc ttttcatgat cagtaacttt acattagata cacataactc
     5641 gcatgtccag gagtttttga taagcgaaaa atacaaagac gacgtagtag ctcaattgaa
     5701 tcgaacgttt aaacaatcga tagaagaata tttctctttt tatgtttttc cagaagagat
     5761 ttcgcaaaag attgcgttga cagtgatgca aaatcacgct cattttgcct attttagagg
     5821 atggatgaat atttttggcc aagaaggatt aaaacagcgg ttaactgaag tagaagataa
     5881 acaattcctg gttttatgtg aaagattggt cagacaaaca tttgctattg ttgattttac
     5941 tgaagtagac gaggaaaatg cccgctttga attatttgct cgctatgcat ctattttacg
     6001 gacgctgatc aagcagttag aattacgtct gcaaattgct tatgatcttc cttatgaaag
     6061 gctaatggcg gatatcgtga aagaaatcat ccaggaacaa ttgctgaata cgatcaaata
     6121 tgatttagtc gaatacgaga aagaaaagca gtatgatatc attttgacta gccaattgaa
     6181 agagtacccg tcacagaagt ccgctaaagt ttttgtattc actagcaacg aatataatta
     6241 tgatttcccc tatttgaacc aatttttaaa agaatgttat ttagaaaaat tgaatttaag
     6301 gatgaataaa acatgaaaat cgtgatcatt ggtgcttccc atgcaggcat cacagctgcc
     6361 ctaaatctaa gaaaacttca accgaaagca gaagttttac tgatcgatga agatcataaa
     6421 gatggactag gctatgtttc aaatggaatc aacctttatt taaaaggaaa gatacgtagt
     6481 ttagctgaag ttgcccataa catgcggact cttcaagaaa gtggggcaaa actgatcacc
     6541 gaatggagag taactgaact tgatccagac aaacatcagc tcatcctcgc atccaaagaa
     6601 ggaaaaaacg agaccataac ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc
     6661 accttgtata aacaaattga agctgaaaat gtctacacct ataaaaatct agtgcaatct
     6721 aagcaagtac ttgccgcttt aaaagaagca aaagaagtag tgatcttcgg tgctggttac
     6781 attggattgg aattagcaga tgctttgaga aataaaggat acattattca tctagttgat
     6841 tatatgccga atgttttatc aagatatttc gataaggata tgatcaacag ttttcaaaat
     6901 cagctgcaaa ctaagcaaat aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa
     6961 tcagaagaga aagtagtatc cgtacaactt ctgtctcaaa caataaaggc agatatggtc
     7021 attttttctg cccaaactcg tcctaatacg acattgttaa aagaaaaagt ggctttgtat
     7081 gaggatgaga ctgtgatagt caatgaatat ctgcagacaa gtgatccgga catctatgcg
     7141 attggagata tcgtcccagt cagttttgac aaaaataaac ggcatctctt tttaccttta
     7201 gttactcgag ctgtacatat ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc
     7261 gcttacgacc tcagacaaaa gataacagca gcagtcatta tagactactt tttaggaact
     7321 gtagggctga cagaagatga agcgccattc ttggaacaaa ataccggttc gtgttcagga
     7381 gaatttgatc tatttccaca atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc
     7441 taccatccgg atacattaga aataattggc ggacagctga taagccaaga attcttatta
     7501 tcagatttga acctgcttgc ggatatcgtg aaacacaaaa ctacgattcc acagctagca
     7561 gttgaggatt ttggatttct gtctgaatac acctctcgtt ttcactacct caatgagtta
     7621 gcttttaaag tactagctaa gagagcaaat agcaatagag tagataaaag actgtgagaa
     7681 atatcacggt cttttattgt gtggggatat aataaatatt aatcaatcga gaataaaaaa
     7741 ttcatataat gaaatagcgg aagaattttt tgagcataat attcgccaaa aatttatcaa
     7801 gtgataaaat tgttgtataa aatgattatt tcaggaggag gagttaaatg tttttagcat
     7861 taaatgaaat tatgcattca aagttacgtt atgcgttagt tgcaggggtg atgtttttga
     7921 tcgcctacct ggtatttttt ttgactgggt tagcttatgg cttagctcaa gataatcgaa
     7981 cagcagtcga caagtgggag gcagattcga ttgtgctttc gaaagatgca aattcaaatc
     8041 tagggatgtc catgattact aagaagatag cagaagaagt cgaaggtggg aaagtggcgt
     8101 atcttgccca aacacctgga gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca
     8161 atgttagctt cttcggtatc gataaaaatc aattcatcat gcccaatctt gttgaaggaa
     8221 aagcttttga taacgatgat gaggcagtag gcgatattag tttgaaagaa gaatatggtc
     8281 tagccatagg agatacagtt aagttatctg gtagtgacaa gacgtttaaa ctaactggct
     8341 ttacggatca tgccaaattc aatgtctcac cagttctcta tacaaccatc aatgcttacc
     8401 aagaaatccg cttcgaaaaa gaagatacaa gtgaaaatgc acggattaac gcaatagttg
     8461 tcagaggtaa aatcagtaat ttacctgaag atttagaaca gatcaaaatt tctaaattca
     8521 tcaatgaatt acctggatat aatgcgcaag tattgacttt tggatttatg atcggatttt
     8581 tgatcgttat tgcggctatt gtgatcggga ttttcattta tgtactgacg atgcaaaaga
     8641 ttaatatctt tggtgtaatg aaagcacaag ggatcacagg aggatttatt gcgcgttctg
     8701 tagtggcaca aacattcatt ttatcatttg ttggtattct tcttggtcta ttaggaacag
     8761 tgggaacatc ccttgttcta cctgatgcgg ttccattcca aagcaattgg ctgttttttg
     8821 gcgtcattag cttgttgatg ttagtagtag ctgtgttagg cgcactattt tctgttcgga
     8881 caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga aggaggaaga acatgaaagc
     8941 aatagaattt aaagaagtag aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt
     9001 gaaagaaaca accatgagta tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg
     9061 ttcaggtaaa agtacttttt tgactttggc aggcggattg cagacgccta gcagtggctc
     9121 tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg
     9181 ttttaaagaa attggcttta ttttgcaagc ttccaatctg gttccttttc tgacagtaaa
     9241 aaagcagtta ttattagtag ataagattaa acatgaacat cgtcaaaaag aagcaaacga
     9301 attatttgag cagcttggcg tggataagtt gatcaacaag tatccagaag aactatctgg
     9361 tggagaacgg caaagggtcg caatcgctcg tgcattgtac aatgatccgt ccattattct
     9421 agcagatgaa ccaactgcta gtttggattc agaaaaggca cgagaagtag tcgatatctt
     9481 ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg gttactcacg atacacggct
     9541 gattgacaaa tgcgatagaa tatttatgat ggaagatggt gtattgaaag aaaaagaata
     9601 accaaaaagc aggtggaaaa atggtccatc gatacaacaa agatacacag caaaaaattt
     9661 tgaaagaagc gactgacttg tttatgtcca agggatatct cggcacttca acaagagaaa
     9721 tcgcacaaaa agttgggatc acccagccca atctctatca ttatttcggt gataaagaaa
     9781 aactatatac ggcagtttta gaaaaccact taaaagatgt tggaaaagct ttgagagaaa
     9841 tcgttcaaac aagcgaaacg ggatttcaag taaccttgac aaaaatggcg cagtttttga
     9901 ttgagacaca tctggtcgat ttgtttctga tgcttcacga tcttgaaagc aatctttcaa
     9961 aggaaacaag agatcatctt ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag
    10021 aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa
    10081 ggcatttttt cttagtgtta gcaccttaca tcactcggtc gaaaagacct gtggaagaac
    10141 cgctaaatgt ggcgcaattg atcgatttat atttgaatgg tgtattagga gaagcgtaaa
    10201 gctgttatat agggagaaaa tagttaggtg aagaagcaag atatgacgaa gatcgttata
    10261 tcttgcttct ttatataggt agtttttcat aaagacaaat ttgttattct ctcatgatta
    10321 tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat ttaataacga
    10381 agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa
    10441 tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt
    10501 tattttctac ggttatatct tttacagccc cttttttgct tacttaagct gattttaagt
    10561 tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat aattggagct
    10621 tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt tggtcaaaaa
    10681 aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac aaaggaatca
    10741 tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg tacactatat
    10801 acagatgact cgctaactac cccacttgca actataaacg gcggagagct ggcggagttt
    10861 ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac tggatattta
    10921 gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga agagctatct
    10981 gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc attaagcaat
    11041 gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt aaaaaaggcg
    11101 ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt agtacagctt
    11161 ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag tttacacgca
    11221 gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta ctatggacaa
    11281 gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct ggattcaaga
    11341 ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc tattttaatc
    11401 gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga cagttatcag
    11461 cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta ataaataagc
    11521 ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt atcgcagatg
    11581 caagcttttg aacaccgttt atttttttag tagcttcaaa gctttttctg tatttgcaaa
    11641 gtgaagtttg gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc
    11701 tacttggtca gatttcgttc cagctcctga tggaagggtg attccagctt cagctgatgc
    11761 tttgtgtaac tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa
    11821 atgatattgt tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa
    11881 acgcgcatat tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg
    11941 tttttctggc tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt
    12001 catccgaacg gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag
    12061 cagtttatag gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt
    12121 taactctttt gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac
    12181 ataagcagca catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc
    12241 taaaacggat aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc
    12301 ttgtttatcc tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa
    12361 catcactttt ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct
    12421 agtataagga atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag
    12481 ttggtcgttt gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat
    12541 aaaactatac cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt
    12601 tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag
    12661 aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg
    12721 gcgcacgaaa aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga
    12781 cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa
    12841 ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat
    12901 gcgctttcgg atcaattgaa gaaacaagaa agaatcttgg agctggaaga agaaacagca
    12961 gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa
    13021 gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt
    13081 aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa
    13141 aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaaa gttagaagag
    13201 aaaggaacgt agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc
    13261 tggagcacgt cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt
    13321 tattgctgcc cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata
    13381 cccagctgtc acgccaacat caaagttagc attttttgat caggtatttg cctttcacct
    13441 agatgaagct ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct
    13501 tacccgattt gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt
    13561 agactggatt gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat
    13621 tttgcagtta cttgcctttg ttccattaga tgtggtccaa aatcaattcg gcgagcatag
    13681 tttcgctcgc tttatcgttg agagaacgcc atttcttacc aacaagatgt atgagctttg
    13741 ggtaacgaat attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag
    13801 agtagattat ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga
    13861 aagataaggt gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa
    13921 acagatggtc agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt
    13981 accagtgagc gaggaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa
    14041 agtgcaacgt ctgcgaggcg ggatccctat acctaaactt gaaaatatcc gtccccatat
    14101 gaaacggatt gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt
    14161 attgtctacg acaagcgaat tgaatcgttt cattgatgat ctttatgaca gtgaaatcga
    14221 atttgcacgt ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg
    14281 tttaaaggaa gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa
    14341 gagcataaga cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat
    14401 cgtccgtggg aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg
    14461 ctacgttatc ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca
    14521 aagtgcttca ggacagactt tatttattga accaaaacaa gtggtggatt tgaataatcg
    14581 tttgcgtcaa taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc
    14641 tgctgaactt gttccccatc gtcaagaaat catccacaat gcttatgtga ttggtaaaat
    14701 ggatctgatg aatgcaaaag cacgatttgg taaagaagta aaagctatcg tgccgggaat
    14761 cagtgaagac aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa
    14821 agtagtttct aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc
    14881 taacacaggt gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca
    14941 agcaggatta ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt
    15001 tgcggatata ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat
    15061 gacgaacact gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga
    15121 gctcggtgca ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga
    15181 tcttggtaaa aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta
    15241 tggatataat cgggcaaata cgattaatgc cagcatggag tttgatgtgg atacattaag
    15301 tccaacgtat cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag
    15361 ccgattaggt cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag
    15421 ccaagattta aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga
    15481 atatctggaa atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga
    15541 agcttacagc tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc
    15601 aaatgaagtc gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa
    15661 aatgcaacag cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac
    15721 ccaattagca aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc
    15781 gaaagaacaa aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag
    15841 agggacgctt ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat
    15901 gaatgtgtcc gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt
    15961 tatccatgct gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg
    16021 aaaacgatac gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc
    16081 tggttatcct caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat
    16141 tactgattat cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca
    16201 aggcggaaat ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg
    16261 gttcgctatc caagtataga aatgctatac tagtcataga ttttaaaaat ggaggtcttt
    16321 gatatgtcac aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta
    16381 ttgattgatt tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat
    16441 cagttggaac aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa
    16501 aatccagaaa ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa
    16561 gatggtcaag tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg
    16621 atcgatcaat atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag
    16681 ttccgtcctc ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta
    16741 taattacgtt gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga
    16801 atatgaaggg gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga
    16861 tcagcccggc tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc
    16921 caaaatatta aaaaatcgtg tccgctcata ttttactggt agccataata cgaaaacgga
    16981 acggcttgtt agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc
    17041 attgctctta gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa
    17101 ggatgataaa acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat
    17161 tactcgtaag gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc
    17221 ggcgaatgag acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag
    17281 tcagacgaag gagccttgtc tttactatca tttgggacaa tgtctgtgtc cttattattt
    17341 cgacgtagat ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg
    17401 aggacacgaa aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat
    17461 ggagtttgaa aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac
    17521 tcgccaaaaa atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga
    17581 taaaggatgg atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga
    17641 tgtttcaatg tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca
    17701 gttttatcag gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga
    17761 caagcccagt gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa
    17821 gaaaaaatta gttgaattgg cgagtaagaa tgcctcagtt gctttgaatg aacggtttga
    17881 tctgattgcc agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat
    17941 gaatatcccg accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa
    18001 tcctgtggct gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa
    18061 atataaaata aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta
    18121 tcgcagatat tcgcgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga
    18181 tggaggaaaa ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga
    18241 tattcctatt gctggcatgg caaaaaacga taaacataaa acaaatgagt tgctattcgg
    18301 tacagatttg gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg
    18361 aatccaagat gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa
    18421 cagttttgct tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct
    18481 gcttaaagag ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga
    18541 aatcggactg ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg
    18601 aaagggcact ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg
    18661 tttacgtttt gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat
    18721 ttcttgaaga aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa
    18781 ctacacagct gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat
    18841 tggccaaaac ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca
    18901 agaccaagga gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat
    18961 cggttatctt ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct
    19021 ttattttgca gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg
    19081 gatggaaaaa tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg
    19141 aaatcagcaa aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt
    19201 ggatgagcca ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt
    19261 ggaattgaaa aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga
    19321 aaaaatctgc gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt
    19381 acatgagatt cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca
    19441 acaggaagta gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt
    19501 agagattacc ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt
    19561 cggctacatt cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa
    19621 agcaggtgaa atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg
    19681 ttaagtcgat ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt
    19741 atgtagcagg aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg
    19801 ataatgagca aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag
    19861 cagttgattc tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt
    19921 tagtagtctc aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag
    19981 gacaaagtac acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag
    20041 cgagtcaatt aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga
    20101 ctcgacaaaa agtcaatttt gattcaaaag gaaaaatgac agtcggagaa gataactcga
    20161 cagtccaata cgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt
    20221 atgcgcagat cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga
    20281 tcctgtccag tacgactgca caaaagcatt tttacggaaa attaacaggt gttttacttg
    20341 ttgcattaac gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca
    20401 aagatatgga aatggtcaaa aatatattag acggtatctc attggataac ttgattggac
    20461 cattcttgtg gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag
    20521 cagctttatg tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag
    20581 tgacttatct atctctaggc ggatacatgc ttggattgat actaggagct tcagatccaa
    20641 acaatatcgt tattcggatc acttcgtata ttccattctt atcttcatat attatgccgg
    20701 ttcgcttagc aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg
    20761 tagccgtaac attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg
    20821 tttatagcga aggcggatta tggagttcat tgaaacaatc gatttcaatt atgaggaatg
    20881 aacgaaaaaa agcataaaac aatcctaaaa tacagaagga agctggacat ctattgttcc
    20941 cagcttcttt ttgtatagac ctattttatt tattttacta tattggatat atattcattg
    21001 aaaatagtgt ataacaactt tataatattg agtataccaa tttttggagg cgttttaatg
    21061 gaccgttatt tagatttact ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa
    21121 atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagtgat
    21181 atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa
    21241 gaaaagatca aagacctttt ttctgaacaa atgacggaaa cggatatcag ccaattcact
    21301 atttttacgg cttatccaga atatgcatta acgactgatt ggtacaaaga acaagataaa
    21361 tcgcagctga tccatcattt gatcgatctt ttacggttta cgacagtcaa atatacccgg
    21421 tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat
    21481 atcgatgacc gtatcaatgg aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt
    21541 ttgatgaagg aagaagaaaa atttttaacg aaacttgcac agacgatcca aaaatctgtc
    21601 atcgatcatt tgcatatcgt tggagatatt tttgaccgag gaactcaagc agccaaagtg
    21661 atggatcgaa tcatggattt ttcttctgta gatatccaat ggggaaatca tgatattttg
    21721 tggattggtg cgtattgcgg ctctgaggca tgtttactga cacttttacg gattgcagca
    21781 cgctataatt atttatttga attagagaaa gaatatgggt tgaatttacg tccattgttt
    21841 tcttttgctc atcaaactta tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat
    21901 ctatccgaaa gagagcaaga agaattagaa aaaatccatc aggcactttc tatccttcaa
    21961 ttcaaatcag aacatcagtt attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa
    22021 ttgctggaaa aaatcgacta tgaagcatca acgattgatt tagaaggtca gctctatgag
    22081 ttgaaaggaa cttgttttca aacgatccag ccagatgatc caaaaaaatt cttggaagag
    22141 gaacaaaaag tgattgatag tttgatgtat tcattccagc actctttgag aatgcaaaaa
    22201 catatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtgctg
    22261 tttcatggct gtatacctgt agacgagaag ggacagctct tagcatttga attagaccaa
    22321 tcttatcgcg gaaaagaact gctgggcttt tttgaaaaac agataactga aagcgcaaaa
    22381 gatctgactg caaaagaaga tttagcaacc gatttgattt ggtatgcgtg gagtgggcca
    22441 ttttcgcctt tattcggcaa gagtaaaatg gcgacttttg aacgttattt tctcacagag
    22501 cctcatacgc acgtcgagaa aagcaatcct tattatcact tgcgagatga agagtggttc
    22561 agtgaaaaaa ttttagcaga attcgacaca aaagaagaag ggtcagcaat cattaatggg
    22621 cacacgccag taaaagtaaa aaaaggggag tcaccgatca aagcaaatgg aaaagtattt
    22681 gtcattgacg gaggtttatc caaagcttat caaaaaacga caggaattgc cggttattca
    22741 ctattatgca attcttatgg tttccaaatc gtgacacatt atccgttttt gcctattgaa
    22801 caacaatttg caaaaaaatc cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta
    22861 ccacgcaaac tcaatcgtga tacaacaaaa ggtatggaac tgcagcagca gattacccag
    22921 ctcaaaaggc tgttggacta tcgaaaggac gattgagaag aattccagtg aacattgttc
    22981 catagactgg agactgagac aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt
    23041 tcttctgcca actcctcggt cacaagtcac actattcaac aaacatgaaa agctgttttt
    23101 tgaaattgct ccttgtgact cagagttgaa cggcaggctc acgacctctt ggtataaacg
    23161 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt gaaaagccat
    23221 tttcacaaaa tttttcttaa tactcgaaac taaacagctt tttcacaacc ccctcagaaa
    23281 gtctattaaa attcattcac aaagaaatcg aataagcgct gttcagatgg atgtacacgg
    23341 tgggtaagtt ccggatgcca ttgaacaccg aggattttat gcatatcatc tttgctttga
    23401 accgcttcaa tgagtccatc gttagaaaaa gcgatggctt ttaataaagg tgaaagatct
    23461 ttgactgcct gatgatggta agaattcaca aaatatggat cggggagaag gttgcttaac
    23521 aaactgtctg aaacaatctt gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt
    23581 tcatgtttta ctgaccactt tggatataat gaaagatctt gataaagtgt accttccaaa
    23641 acgacattca atagttggag tccccggcag actgcaaaaa ttggttttct ttgtttcaat
    23701 gcttccaaga tcagtgcctg ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt
    23761 ggatgaggat cttccatgta aagttgtgga ctgacatctt gaccacctgc aagaataagt
    23821 ttgtcgattt gtccaatcag ttgaggagct gaatctggag acatgactgg cagaataaga
    23881 ggcaatcctt gagcatctaa aactgcatcc acgaatcctt gcggtgtata gctcacttga
    23941 tttccttgga aagtatctgt tgctcgaatc agttgatttc cggcaatgcc gatgattggt
    24001 ttcatttgta ttccccctaa agtagactta ttgtaacaag gatttaaaaa aaattaaact
    24061 atgtaagact tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaaat
    24121 ttcgtgtgaa attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta
    24181 agcagaaata aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag
    24241 gacattttat tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat
    24301 gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag
    24361 ctttggaaca aatccgaaaa gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc
    24421 agcagctgaa gatctatgca acagaaaaag agcaggaatt ggaaacgatc caaacaaact
    24481 atcgacaaca actaaaacag ttggtggaag aaaaagaaca atttgaaata caagaattag
    24541 aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagatc
    24601 aatatcaaaa gcatcagagt caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt
    24661 atggccgtca ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg
    24721 attttacaag cagtacaagg aatccaaaac gttgaaatcc gggatctgta tcaaaataga
    24781 acaaacaacc agtgggtgga gaagtatttt ccagatacca cgattattga taaagaagca
    24841 aaattaagcg ctttaggtaa tcggttgaaa gacatccaag aagctatcca attcatacaa
    24901 caccacggcg atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa
    24961 gatttggaag ccaattattc cgaagaaaat ttcataaata aattgaaaga agtccttgat
    25021 ttaaaagaaa agtgggaaca actaactgaa gaacgtgatc gtttagaaga agaggaagaa
    25081 tggcttcttc attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc
    25141 atttttgaga tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactgagt
    25201 aagcttgatg acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg
    25261 tatgttgctt taaaaaaatc agttgctgtt atagaagaaa ttgctgggaa atacgggttc
    25321 aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag
    25381 caattatcca ttgttcacga gcagcaaaag aagataacga cagcgttagg tcagtgcagt
    25441 ggctatatcc aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgagaa
    25501 aaaataaaag aacggtttat ccatgcgact tatctgatcg ttttgcaagg ttgggtagat
    25561 gcagaagaaa aacagaattt gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata
    25621 tacgtcagtt tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg
    25681 aaaaaccatc cgcttgtcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa
    25741 tatgaagaag tagatccaac accttggatg acaccatttt atcttgtctt cttcgggatg
    25801 atggtcgcag atgttggtta tggtttgctg atgctcattg ggacaatact agctcaaaaa
    25861 ctacttgttc taccacgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcgata
    25921 ccatcgatca tctggggatt gatctacagt tcatttttcg gacagtcatt accaaaagaa
    25981 ctgtttggca tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt
    26041 ttgattttat ccgtgatttt cggtctgata cagattttgg ttggattatt tgtggcagca
    26101 aaggaaaata tcaggagaaa agattacttg agtgctgtca gtgatggatt tgcctggcaa
    26161 gggatcctaa ttgggatcgt tattgcgttg gttggtgcca tgttgataaa aaatacagca
    26221 tttgtttacc ttggcgctgg gctatcgatc attgctgcct tatgtatctt gatcgttccg
    26281 attatccaaa cgccgtctaa agtcaaaggt gctgccaaag gtgcgtataa tctttacgga
    26341 ctgaccggct atatcggtga tttagtcagt tatacacgtt tgatggcatt gggtatttca
    26401 ggcggaagta ttggagcggc attcaatatg ctagttgctt tcatgccgcc agctgctcga
    26461 ttcagtgtag ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg
    26521 ctaagtgcct atgtccatgg cgcacgattg cagtatgtcg aattttttgg gaagttttat
    26581 acaggaggcg gacgtgcctt tgatccgctg aaaacggcgg aaaaatacgt caacatcaat
    26641 cacaagaaaa aaaacaataa ataattggag gatttttata taatgatgga ttacttaatt
    26701 agtcaaaatg gtggaatggt atttgcggta ttagcgatgg caacagcgac gattttttca
    26761 gggatcggtt cagctaaagg tgttggaatg acaggtgagg cagcagcagc attgacaaca
    26821 agtcaacccg aaaaattcgg gcaggctctg attctgcaat tgcttccagg tacgcaaggt
    26881 ttgtatggat tcgttattgc ctttttgatt tttatcaact taggaaatga catgtcagtt
    26941 gtacaaggac tgaactttct aggtgcttct ctgccgattg cttttacagg cttgttttca
    27001 ggtattgccc aaggaaaagt cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa
    27061 catgctacaa aaggaatcat ttttgctgcg atggttgaaa catacgcgat tttaggtttc
    27121 gttatttcct tcctattagt gttgaatgcc taagcataaa ggaggaaaag cagatgaacg
    27181 ccattgaaaa aatcatctct cagatgaacg aagcagcaga gcaggaaaga gcggcgttag
    27241 aacaagaaga gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caagttgaaa
    27301 cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc
    27361 taaggaatcg ccagcaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt
    27421 taagacgatt gtttgcagat gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa
    27481 tccagtttat caaaaatgca ttgtattcat tacctttgac aggaaaagta gcattcattg
    27541 ctggagaaaa atcagcagcc tatctttctc agacgttgtt ggatgaatgg aacaatgaac
    27601 ttccctttat gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa
    27661 acgatcaagg agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa
    27721 cgatgagttt tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata
    27781 ccacgaacta aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt
    27841 tgaacggatg attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat
    27901 ttacagtcca tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga
    27961 gcagagccgt ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat
    28021 ctatacgatg cgttatactt tccataatct gaaggttttg acaaaagctg agatgacaaa
    28081 tcaaaatctg gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga
    28141 tgccattcat acgcaagttt caaatgaact tcctgaaagc attatggaca gtatacgaga
    28201 agtacgtgat tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg
    28261 acagtttttg acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac
    28321 agaagtcatt gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca
    28381 gaaaagaaca tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga
    28441 agtatttttg aactttgtag ataaagatat ggaagattac acgaattttc tattagagac
    28501 agattatgct gattttctgc gccctgtcat ccgtgaggga aatattgatc tcgtaaagct
    28561 tgaacagttg aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt
    28621 cggaccactg ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg
    28681 tttactggtt atcggcaaaa gaagccattt cgaatcagaa acaatcagaa agcgggtgag
    28741 acaagtctat gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca
    28801 aattgtttgg ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg
    28861 aagaaatggc aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga
    28921 gcccagaaac gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca
    28981 atcatcaagg atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag
    29041 taggtcaaaa tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca
    29101 taaaagtctc cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca
    29161 tgtgtctagt gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg
    29221 cgtctattca agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta
    29281 ctggtgaagc tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa
    29341 tccaacggcc attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag
    29401 ttcaattgcc cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg
    29461 gagaaacggt gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac
    29521 ataaaatcat ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat
    29581 tcactattga tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga
    29641 tgcttcaaaa atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg
    29701 cacccatgat cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag
    29761 cagcagctgt acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa
    29821 aatgggccga tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga
    29881 cggatgtttt aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg
    29941 aacgtacggt attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta
    30001 tctatacggg aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca
    30061 tggcagactc cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag
    30121 aaatgcctgg tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg
    30181 aacgatctgg aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa
    30241 tcagtgctgt atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc
    30301 gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt
    30361 cgatcgattg gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc
    30421 atatccttca agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag
    30481 aacagcagct gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc
    30541 gtctgacatt agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg
    30601 atgatgtcga tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga
    30661 cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg
    30721 acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat
    30781 tagaaaaact agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag
    30841 aaggaggcat gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg
    30901 atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa
    30961 aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa
    31021 atattcgaag gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat
    31081 cctttagaac taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga
    31141 ccgaaagata acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta
    31201 atcaatccag tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc
    31261 gatcatttga atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt
    31321 cctcataaag aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat
    31381 gatttcgccg tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg
    31441 gaagattttc gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac
    31501 gaccctgcaa tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt
    31561 gcttatgaaa agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag
    31621 gcattgcgag agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg
    31681 tatctttaca cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa
    31741 ggatcggtta ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc
    31801 cctgacttga ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag
    31861 agtgggattc agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga
    31921 actggacccg gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc
    31981 tatgcacaag gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct
    32041 gatgtagaca agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa
    32101 ggattctaca cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg
    32161 atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca
    32221 gaagggaagt gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact
    32281 cgattgaaaa aacagttgac aactgccaca agagggcata aattattaaa agataagcaa
    32341 gatgaactga tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa
    32401 atggaagagt ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg
    32461 gaacctttca tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta
    32521 gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg
    32581 acagacacac ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc
    32641 gatcggttta ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt
    32701 caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg
    32761 acgatccctc aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag
    32821 cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta
    32881 tcttttttta aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc
    32941 cggctgttca gcttctcgca ttaggatttt ttctcctgat tttagttggc ggcggcctac
    33001 ttaccctacc attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca
    33061 ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga
    33121 acagtgctgg tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga
    33181 tggttccgat tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta
    33241 tattaaaaga agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca
    33301 tcctaaaatt ggcctttgtg atccaattga ttggtacggt tgctttatct tttgtatttg
    33361 tgcctgaatt tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt
    33421 tttgtaatgc agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt
    33481 atttgatcat ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc
    33541 gtgatttact ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa
    33601 ccgttacagg taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg
    33661 gggcaacgct agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg
    33721 tgacacctag aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc
    33781 tgattttaac gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat
    33841 tgaaaacaac tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta
    33901 cgagagcgga agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt
    33961 tattttttgt tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa
    34021 cgatacctaa aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta
    34081 cagtaggtct tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta
    34141 tagcgcttat gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa
    34201 aagcacaaag aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat
    34261 ttcatcttct gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt
    34321 gcttcttgga tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt
    34381 gaacctcggg tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt
    34441 tatcaatagg ttataattca tattttttta ttttttcttt tacgatactt agtccagaag
    34501 ctaaaatttc aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgtac
    34561 gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa
    34621 ttatcttagg gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg
    34681 ttggtttttc tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta
    34741 atttagtttc taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca
    34801 catctaatcc agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga
    34861 catgtactgg aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc
    34921 cagctaagta gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt
    34981 gatagtaagt tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt
    35041 caatcacttc ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat
    35101 tacaagaagg cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata
    35161 tatcaggtcg cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact
    35221 gagccaattc aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag
    35281 agaaaagtgc agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag
    35341 attctaagga cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa
    35401 aatttgtgcc tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag
    35461 ttgattgaat aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa
    35521 aatcaatgat atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg
    35581 gagaaataaa tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt
    35641 tataagcctt gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg
    35701 agggtaactg agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa
    35761 tcaacgatcg ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt
    35821 aattgtcata agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt
    35881 ttaccattga ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga
    35941 aaattattaa ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa
    36001 tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg
    36061 aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg
    36121 agaacagata ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt
    36181 taccttctca tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat
    36241 tgataaacgc aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag
    36301 attacttagc aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat
    36361 tgtcgcattt gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata
    36421 ttttagaaga aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa
    36481 cgtttataaa tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt
    36541 tgacctctag taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca
    36601 atgctgtggg cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt
    36661 ctcaaatcta tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag
    36721 ccatggcaga aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt
    36781 ctgagaaaaa aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc
    36841 tagctattga agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt
    36901 taaaatgaac ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa
    36961 tccagcagcg gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc
    37021 cattgaaaac aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct
    37081 tagatggttt acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc
    37141 atttgtttta tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag
    37201 cggtaatcta tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat
    37261 gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga
    37321 agcctactta gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt
    37381 gcagccagat tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc
    37441 agaaagtgtg caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga
    37501 agatccagta tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa
    37561 taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt
    37621 aaaagaagat cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt
    37681 tatttgattt ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct
    37741 acaatggttg ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat
    37801 tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc
    37861 ctcggatgac ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag
    37921 tgccaggtaa tccttttaag tcagtaatct caagaccagc agctttatgt tgctcgtgta
    37981 atttttctag atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg
    38041 caccatgatc atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat
    38101 aaacaagtgt aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg
    38161 ccttttgata aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca
    38221 ttttcataga ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa
    38281 atagttgatt tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac
    38341 tgttaaggtg gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg
    38401 attatcaaac ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa
    38461 tcgaggctcc aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga
    38521 aagaagaagc gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt
    38581 cttatctggg agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata
    38641 atccaggata tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga
    38701 ccaataaaat ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc
    38761 accgatgggc agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat
    38821 cagctttctc tcagtttttt tatttattac agtacatatt ttttgattgc ttcagctaca
    38881 ccgtgctcgt tgtttgatga agtgatgaca tttgctgctt ctttgacaag agaaacggca
    38941 tttcccattg ctactcctag accagcatat tcaatcatgg ataagtcgtt ctcgttatct
    39001 ccgatagcca tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc
    39061 gcacctttac ttgcttcttt atttagtact tcgaagtaat agggcgcact tttgaccgtt
    39121 gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt
    39181 tcgtcgatca tcatcatttt gacgatgttc atgtctggtg tcatttcttc aggtgttcga
    39241 tattttagcg gcatcttgac taaacttgct tcgtggatcg tataagcgct gatatctcga
    39301 ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat gactgccgac acggcgtgcc
    39361 atcacttcga tctctaggaa atcttcatgt gtcagtccgt gacgggcgat gattttacct
    39421 gatttcgttt gttgtacgag tgccccgtta taagtaataa cgtaatcatt atcttgaaaa
    39481 aggttcaact cgtttaactg ttcctggaca cctggtaagg gacggccagt gcataaaacg
    39541 atcgaaacac cttttgcaac agcagcggca atcgcttctt ttacttcttt tgttacttct
    39601 cttttctcat ttaataaagt accatcgatg tcgatagcga ctagtttgat agacataatt
    39661 tcctccaata tattttaatt tatttcaatc aatgcgccat tacggatatg gctggcaaat
    39721 tcttgataag tttcgtcaaa taaatcataa tgatcacgca aggacggatc aaccatttct
    39781 tttggaaaga aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc aacaagctga
    39841 ctaacctgtg ataattctat caaagttccg tcatttcgca aaatctcgat ttgtgtgcga
    39901 tgtctgcctt gttctgggcg ataaaaatca taaggtagat cgtagctaga attcacagca
    39961 gtgtaataaa caggattata tccgaccttt tctactaaca aagtgagttc ttggactaat
    40021 tttgaatccc ggttgccaga aaaagtagca gatttcagtg gtttacgatt caaaaaacgt
    40081 ttagctaaat catttaaaat aggatcagca gattcagacc attgagtaaa gtaagtgccc
    40141 aagaccccat catcaagttt gaggtattct tctaaagtaa agcttccgtc aagaaatggc
    40201 agaagtaatt gagaatggag ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg
    40261 cgatgaagca gatgctccaa aattacttcc ataccacggg aaacaggatg gaagtacact
    40321 tgcacataca tttgatatcg gctaacgata taatcttcca ctgcatgcat gccactcatg
    40381 gaaaaagcga tcccgccttc ataaggacga atcacacgta atattcgggt aagatcaaat
    40441 gttccatatt ctgttccagt gaaataagca tcccgaagca aatagtccat tcgatctgca
    40501 tcgatctgac tagaaatcat ttggacaact tgtgggttgg ggtaagtttt ttggatcaca
    40561 ctagctacct tttcaggaaa accttcttct actcggttaa gaatctgata cacttctgtt
    40621 tctggagaag tgatgatctc tacagtgatg gcttcatgat tcgtatggaa gatatgttca
    40681 aatgtatgag agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc
    40741 agccgttcct gatcatccca gcctccgttg cctatttttt cttttgaaaa atttcgagaa
    40801 aagatatcgc agattcgtct tgaaatctca taaacgccta atgaatgagt aaaacggcta
    40861 tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt
    40921 tggacttctt tcgaattgat caaatctaag attacttgat gctggacatg gatatagtta
    40981 tggactggat cacgaaaaac tttttccata ggcagcattt gatatttata aggggttgtc
    41041 ataatgtagc tcctttatgt tattacgttg ttccattctc tggatttggt tgttccattg
    41101 ctccagctgg atttcttttt ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata
    41161 cagcttttca aaagcttttg caaatgcttc tttgacttct tttacaaaaa ggggttggtc
    41221 aagcagttct tcaagtgttg ccattgtagc aggatccact gcaggatagc catttgtacc
    41281 aaagtcttct tttaaagcag attgataaaa tcgcttcatg acttgcccgc gtttttcctg
    41341 attaccgttg acacttaagt acatcataac agaaatccca tttttgatcc gacgctgagc
    41401 gataccggca aattttttgc catggatgct taaatcataa gttccaggac aataagaatg
    41461 ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg tatttagtca ataggagcat
    41521 ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca ctttggggga aaatcaaaga
    41581 aatattcaaa attccttcat ctgatacgat ccctaatcca cctgaatttc tgatcactgg
    41641 agcgtaacct gcatctttca gtgtttggat cccgtcactt agaaaaggag ttcgaacatc
    41701 cttcatacct aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg
    41761 ttctttagaa tattccgtta atatatctgt taatgcaaaa ggggaaaagt cgtttttttg
    41821 ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa ccggctcctt tgtataaatt
    41881 catattttct acaatattat actctaaaat caaagagttg ttttggaaaa aaggttttct
    41941 gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta ggaacaatgt agctatgaga
    42001 agtaaaggag aatgaataat ggaattatca caaggaacac cgatttcaat caaattgcgt
    42061 acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg
    42121 gtgacaattg gcgatacgct gtacatacgt tataaagaga ttcaggagga gacgggcgat
    42181 gagattcctg taacgatcaa attagttcca gatggtcatg tacagctgat ccgtgcagga
    42241 gaaatgcgga tgcgtttgaa atttggctat aaagaacggt tagaaacatc ctatcgaaca
    42301 ccatacggta tgatccagat tgctactttt acgaaagaac tgcatgttag tttgaaagac
    42361 cgtcccacag cagggaaggt tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc
    42421 ggcgaatatt atttgacctt ggattttaca gcgtaaatat aaaagaaaag tttgattttt
    42481 tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt
    42541 agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc
    42601 ttagaacaac gtggcgatgt catggatttt tctgatttag ctaaccagat ccaaaactat
    42661 cttgggaaat cagacagtga aattcgtgat tcactcgcac aattttatac tgacttgaat
    42721 attgacggca gctttatctc attaggggat aatcgttggg gcttgcgttc atggtatgct
    42781 atcgattcga tcgatgaaga agtaaaccat ggaatggacg aagaggatga agatactcca
    42841 cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat
    42901 tacaatgacg atgacccaga agatacagat ctgacagaag aagatgacga tgatctgttt
    42961 gatgacgatg atgatgaaga tgaagaaatc gctgcttata attctgactt gcaagaaatc
    43021 ggagcagatg acccgacaga tgatgaagaa gacttgccag gaaatatcga agaagattta
    43081 agtatcatag acgaagacga tgatgatgaa gattatgaag aggaagaatt ttctgactct
    43141 gaaaaagaat aaacattatt ttcttacgag aggttgtgac agagacgatc agcctatcag
    43201 aataaggagg tgtgacacga tttttgtcac accttctttt ttgtatataa aaatcccaag
    43261 taaatacata aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt catattaagt
    43321 gaggcagaaa ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa
    43381 aataccgtca cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca
    43441 cgatgaatta cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata
    43501 aagccctgaa ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc
    43561 ggatattgat ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact
    43621 tatttgaaat tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc
    43681 atatgatttt agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg
    43741 aaggatttcg tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa
    43801 cgaaaatgta ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt
    43861 gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca
    43921 attcccaaag ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt
    43981 ataaaagcaa agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta
    44041 atgattacaa caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta
    44101 cccatcttag gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa
    44161 aaaatcgata ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt
    44221 catggattaa atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa
    44281 gaatatggaa attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta
    44341 gacgataacc ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat
    44401 gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt
    44461 ttattcaaag atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc
    44521 gaacaagtca aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg
    44581 acgatagaaa aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat
    44641 caattagttt tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa
    44701 tttgtcattc ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat
    44761 tagacgagaa gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata
    44821 agaagagaaa aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta
    44881 ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag
    44941 taaaaggaca aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat
    45001 caaccattaa aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag
    45061 cggaaaaggt acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag
    45121 atccggctgt caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac
    45181 cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag
    45241 aaaatacggc gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg
    45301 gcaatcacac atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg
    45361 aagtgacgaa agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc
    45421 gtccgcccta tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg
    45481 aatggtctgt tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga
    45541 tccaagcaac agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga
    45601 ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag
    45661 ttggggattt attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc
    45721 ggccagttca gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg
    45781 aacacataac taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac
    45841 tgtccaccaa ttttctttgg cggacagttt tcacttaaaa ataaagaagc aactattcgt
    45901 aaattatgct aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa
    45961 ccggaatctt acgtgatatc cactacacta aggacgcaag cactgggata attgtaacta
    46021 aacagataaa aaattacaag gggattggac aaaaaaatga aatttgagca aaatttttca
    46081 caaaaacagc agcagacaca gaaactagcc atgacgcaac agttgcaaca gtccatccaa
    46141 gttttacaat ataacagtga ggagctgttg gcattcgttg agaatcaagc aatggaaaat
    46201 ccattggtgg aagttgttga gccggaatgg cagccagatt acataaaagc ttcttcatct
    46261 tcttatgaag gagaagagac aaattaccta aatcagattc ctgatacaaa agggtcttta
    46321 tttgattcat tgatcgaaca agtccatttg aattaccgag atactttttt gaggaaaatc
    46381 gttctttatc ttgtagaata catcgatttg aacggatttt tgacgattag tttagaagaa
    46441 gccggaaaag agattggagc tacgcctatc caaatgctcg atgcgttgac tctgattcag
    46501 caattagaac cagctggagt aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca
    46561 gaacgagatg attatgcgcc ggaattagct tatatcgtgt tggaagaatg ttttgaggaa
    46621 ctggtggagc gaaaatggaa agaaatcgca caacgttttt ctgttgatct acatgctgtt
    46681 cagcagatat tcgactattt acaaacgttg agtccaagcc ctggccggat tttcgaccgt
    46741 agcagcgaat tgtacattat tcctgatgtc cgggtattag tggacgacga taaaaatgta
    46801 caagttatct caaaccgaaa aaatcagcca aatatacgct ttcaagagag ttacttcaaa
    46861 caaatgtcac aacaagcaga taaagaaact gaaaattatt taaaagaacg gaaacaggaa
    46921 ttcgaatggt tgaagaaaac gatcctacaa cgtggggata caattttgcg ggttgctcaa
    46981 gtcattgttt cccggcaaaa agctttcttt atcgacaagg aacgtccaat caagcctctt
    47041 acgttaaagg aagttgcaac tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc
    47101 aacgggaaat atttggaaac agacttcggt gtatttgaat taaaaaaatt tttcactaca
    47161 cgaattccta cgaatagtac agaacagaca gaagaccttt cagcagatac agcgaagaaa
    47221 aagctgcagg aattagtcga tcaagaagac aaaaacaagc cgttgtctga ccagaaattg
    47281 gttgaattgc tgaaaaaaga tgagatagcg atctcaagac gaacagtagc aaaatatcgt
    47341 gatttattag gaatcccgag ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa
    47401 agactagaaa aagtatttca ctttttctag tctttttagt tgaaaaacga gagcaaaact
    47461 gttagactta cagtcgtgag gttgagattt ccctttattg atgtgaaatt cgaatagtta
    47521 atttcttttt ttatcgtata tgggtcgttt taagtctaca ttggacgtaa agtgtccaac
    47581 taaggagatc gctatgctgg atgaaataaa aatgattgaa tctgttgcac cagatattat
    47641 cgaagtgttg caagaacgct ataaaatttt acgaaatatc tattggatgc agccgattgg
    47701 acgtcgaagt ttatcggaaa gtatggggat cacagaacga gtattgcgaa cagaaacaga
    47761 tcttttgaaa aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa
    47821 gggcgaagaa gtttatcaaa gccttgaaaa cttcatggat cagcttttag gtacgcatca
    47881 aaccgaacaa cagttggcag aatattttgg taaccaacgc tgtatcgttg tttcaggaaa
    47941 cagcgaagag cagaccaaag tggctgatgc attcggcgaa gcgctgagcg aagccctaga
    48001 ccgactactt ccagaaggcg agaatatcat cgctgtgatg ggaggaacta ctatggctgt
    48061 ggtagccgaa caattgtcta atctggaaaa taagaaacgg cataacctgt ttgtccctgc
    48121 aagaggcggg attggtgaag cgataactgt acaggcaaat tctgtcagtg caagaatggc
    48181 tgctaaagca aacggtaacc accgtgcact ttatgttccg gaacaattaa gtctggcaac
    48241 ttacaattct ttactcaatg agccatctat ccaagaagtc ttaaacttga ttagtgaagc
    48301 gaattgtgtt attcatagta ttgggcgtgc attacacatg gcagcacgtc gtaagatgac
    48361 tgaaaaagaa ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt ttggctactt
    48421 tttcaatgaa aaaggtgaag tcgtttataa ggtccctaga atcggcatag aattgcaaga
    48481 tttggaaaaa gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat
    48541 tcgagcttat atgaaaaatg caccaaaaca aacgtggctc atcactgatg aagctgctgc
    48601 aaatgagatt ttaaaagggg taacccttta aaataaatat ttttttgatt ttcataagga
    48661 ggaaatctta aatgacagtt aaagtaggta ttaatggttt tggacgtatt ggacgtctag
    48721 cattccgtcg tatccaagat gtagatggaa tcgaagtagt agcaatcaac gacttgacag
    48781 atgctaaaat gttggcacac ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa
    48841 cagttgaagt tcatgaaggt tcattcaacg taaacggaaa agaagttaaa gtattagcta
    48901 accgcaaccc agaagaatta ccatggggcg aactaggcgt agatatcgtt ctagaatgta
    48961 ctggtttctt cacttctaaa tcagcagctg aaaaacattt aacagctggt gctaaacgcg
    49021 ttgttatctc tgctcctggc ggaaacgatg ttccaacaat cgtttataac acaaaccacg
    49081 aaacattaac tggtaaagaa acagttatct caggtgcttc atgtactact aactgtttag
    49141 ctcctatggc taaaacattg aacgacaaat ttggtgttgt tgaaggatta atgacaacta
    49201 tccacgctta cacaggtgac caaatgactc tagacggacc tcatcctaaa ggtgacttcc
    49261 gccgtgcacg cgctgctgct gcaaacatcg ttcctaactc aacaggtgct gctaaagcta
    49321 tcggtttagt aattcctgaa ttgaacggta aattagacgg agctgctcaa cgtgttcctg
    49381 taccaacagg ttcattaaca gaattagtaa cagttcttga aaaagaagta actgttgacg
    49441 aaatcaatgc agcaatgaaa gaagcttcaa acgaatctta cggatacaac acagacgaaa
    49501 tcgtttcttc agatatcgtt ggtatgactt acggttcatt atttgatgct acacaaacta
    49561 aagtaatgac agttggcgac aaacaattag ttaaaactgt tgcttggtac gacaacgaaa
    49621 tgtcatacac tgcacaatta gtacgtactt tagaatactt cgctaactta taagatagac
    49681 aaacgtacaa gtgtaaatta tagacatgta catttaaaag cggggaagca atcgcgcttc
    49741 ttccgctttt tttgataata agtttattca ggaggaaata aaatggctaa gaaaacagtg
    49801 aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg
    49861 aaagacggcg tgatcactga cgacactcgt atcaaagcag cattgccaac aatcaactac
    49921 gttctagaac aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa
    49981 gaagataaag aaggaaaatc tttagctcca gttgcaaaac gtctaggcga attattaggt
    50041 aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat tagaagaagc aatccgcaac
    50101 atgaatgacg gcgacgttgt cgtattcgaa aatacacgat ttgaagatat tgacggtaaa
    50161 aaagaaagca aaaatgatac agaactaggt aaatactggg cttcattagg cgatgtattt
    50221 gttaacgatg ctttcggtac agctcaccgt gcacatgctt ctaacgtagg tatcgcttca
    50281 actggtatcc caacagttgc tggattcttg atggaaaaag aaatcaagtt catcggtgaa
    50341 gcagtagaag aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa agtttctgat
    50401 aaaatcggcg taatcgaaaa cttgattcct aaagcagata aaatcttgat cggtggcgga
    50461 atgacttaca cattctacga agcaaaagga atcaagatcg gtaactctct agtagaagct
    50521 gataaagtgg aactagcaaa agaattaatc gaaaaagcag gcgacaaact tgtcttgcca
    50581 atcgacaatg tctgtgcacc agaattttca aatgatgttg aaacacaagt aatcgaagga
    50641 gacattcctg atggtttgat ggctttagac atcggcccag catcagtaaa attatttgct
    50701 gatacattac aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt atttgaaatg
    50761 agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca
    50821 acaacaatca tcggtggcgg agactcagcc gcagcagcag aacaattagg atttgctgac
    50881 aaattcacac acatctcaac aggtggcggc gcaagcttgg aattgcttga aggtaaagaa
    50941 ttaccaggac tagcagcaat caacgataaa taaacgacca agtttgctca attctgacga
    51001 gtaatcgaac aaaaggtgaa gggagttttc atgactccct agccattttt caataaatta
    51061 aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac
    51121 tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa
    51181 agttgattca gtcatcggat ctcctgcatt attcttacaa gaattaatga catcagctga
    51241 aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac
    51301 tggtgaaact tcaccagcag ctcttgctga tctaggtgtt cattatgtga tcatcggaca
    51361 ttcagaacgt cgtgaatatt tccatgaaac agacgaagac atcaataaaa aagcgaaagc
    51421 tatctttgca aatggtatga caccaatcct atgctgtggc gaatctttag aaacctatga
    51481 agcaggcaaa actgctgaat ggatcgaagg acaaattaca gctggtttag ctgatctttc
    51541 agctgaacag gtgtcaaaca tggttattgc ttatgaacca atctgggcaa tcggtactgg
    51601 taaatcagct gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc aaacagttga
    51661 aaaactttat ggaaaagaag tatcagaagc agttcgtatc caatacggcg gttctgtaaa
    51721 accagaaaat attgctgaat acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg
    51781 cgcaagcttg caagcagatt cattcttgtc attgttagac gctgtaaaat aattgttgaa
    51841 aatttgcgaa aaatgcaaga aatgtttgca ctaatgagca aaattaacta aaatcgtata
    51901 tgagggaaat cccttaggaa aaaacaaact caaaggagag acaaaacatg tcaattatta
    51961 ctgatgttta cgctcgcgag atcttagact cacgtggtaa cccaacaatc gaagtagaag
    52021 tatacacaga aagcggagct tttggccgcg gaatggttcc ttcaggtgct tcaactggtg
    52081 aatacgaagc agttgaatta cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa
    52141 ctaaagctgt cgacaacgta aacaacatca ttgctgaagc aatcattggc tacgatgttc
    52201 gtgaccaaat ggctatcgac aaagctatga tcgctttaga cggaactcct aacaaaggta
    52261 aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact
    52321 acctagaagt acctttatac cactacctag gcggattcaa cacaaaagta ttgccaacac
    52381 caatgatgaa catcatcaac ggcggatcac atgctgataa ctcaatcgac ttccaagaat
    52441 tcatgatcat gcctgttggc gctccaacat tcaaagaagc tctacgtatg ggtgctgaag
    52501 tattccacgc attagcttca atcttgaaag ctcgcggatt agcaacttct gttggtgacg
    52561 aaggtggatt tgctccaaac cttggttcaa acgaagaagg ttttgaagta atcatcgaag
    52621 caatcgaaaa agctggctat gtacctggta aagacgttgt tcttgctatg gatgctgctt
    52681 cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact agctgattca ggcgaaggcg
    52741 aaaaaacaac tgacgaaatg atcaaattct acgaagaatt agtttctaaa tatccaatca
    52801 tctcaatcga agacggatta gacgaaaacg actgggacgg attcaagaaa ttaactgacg
    52861 tattaggcga caaagttcaa ttagttggtg acgacttgtt cgtaacaaac actcaaaaat
    52921 tatctgaagg tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg aaccaaatcg
    52981 gtacattaac agaaactttc gaagctatcg aaatggctaa agaagctggc tacacagcag
    53041 ttgtatctca ccgttctggt gaaacagaag attcaacaat ctctgatatc gctgttgcaa
    53101 caaacgctgg ccaaatcaaa actggttctc tatcacgtac tgaccgtatt gctaaataca
    53161 accaattact acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat
    53221 ctttctacaa cttaaaaaac aaataattct tttctaatga agatacatga tcgactattg
    53281 aaagatagtc gagtatgatc acttagttaa gtaatcgaac tctctacttg ctgaaatatc
    53341 agcaggtaga gagttttttt gcaactagct tctgtatatg atgtttacaa agaatggtac
    53401 aagaaaagat catccttgaa aaaggaaagc taacaataac tagcatcata taagagcttt
    53461 taaggataga acagtagaat aggaatctat gagcaagtcc tctcgtataa ataaaatagg
    53521 agggaagtag cagcatattt ttcataaagt ggctctcttt cttcattttt actttggttg
    53581 ctcctatttg aaaattcgag cacactcaaa tacaataaaa agaaatggca agggagagga
    53641 ttagatgaaa aaagaagaaa aaatcgcgat tttacaagag atcatccgaa ttaaatcagt
    53701 gaatggaaac gaaggtgaag tagcagccta tttgaataag cttttggcaa agcatgacat
    53761 aacaggagag attgtttctt acagagatgg tagagataat ttgattgctc gttaccaaaa
    53821 gggccaatca ggaaaagtgt taggcctatc tgggcatatg gatgtcgtgg cggctggtga
    53881 tgaatcgtct tggacctatg caccctttgc agcagaaatc catgggaacc gtttgtacgg
    53941 acgtggtgct acagatatga agagtggctt ggcagcaatg gtgattgcca tgatcgaact
    54001 gaaagagtca ggaaaaccat ttaacggaac agtcaaatta ttagccactg tcggagaaga
    54061 agtgggtgaa ctaggtgggg aacagctcac gaaagcaggc tatgtagatg acttagatgc
    54121 cttgatcatc ggagagccaa ccaattacag tttgatgtat acgcacatgg gatcaatcaa
    54181 ctatacagtc acctcgcacg gaaaagaagc gcatagctct atgccagacc aaggttataa
    54241 tgcaatcaat cacctaaatg aattcattac gaaagcaaat gctgaaatga atcacctcgc
    54301 tgaaacaatc gagaatcctg tcctaggaaa aacaatccat aatgttacat tgatcagtgg
    54361 cgggaatcaa gtcaacagca tcccaagcca tgcgcagctc caagggaaca ttcgatcgat
    54421 tccagaatac ccaaatgata aaatcattgc attgctgcaa tcaatcgtca atgaattgaa
    54481 tcaagagaca gactatcatt tggaattgat gatcgattac aacaaaatac ctgtcaaagc
    54541 tgatccagat tctcctttga ttcattgcat tcagcaacaa ttcagccagc cattaccttt
    54601 agttggagcg gcagcaacaa cggatgcagc cgaatttacg aaagctaacc attccttcga
    54661 ctttgtcgtc tttgggcctg gtgtcgttac tttgcctcat caagtagatg agtatgttga
    54721 aatcgataat tatttggata tgatcgaaaa atatcaagga attattttat cctacttagc
    54781 gtaagatttt tttaataaat gaaaaaacag agccagatta gcat
//
LOCUS       DADVXO010000004        52541 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.004, whole genome shotgun sequence.
ACCESSION   DADVXO010000004 DADVXO010000000
VERSION     DADVXO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 52541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.004"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            165..1676
                     /locus_tag="KB950_000193"
     CDS             165..1676
                     /locus_tag="KB950_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBB6856439.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            1688..2647
                     /locus_tag="KB950_000194"
     CDS             1688..2647
                     /locus_tag="KB950_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBB6856440.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            2733..3812
                     /locus_tag="KB950_000195"
     CDS             2733..3812
                     /locus_tag="KB950_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6856441.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(4064..5464)
                     /locus_tag="KB950_000196"
     CDS             complement(4064..5464)
                     /locus_tag="KB950_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBB6856442.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            5682..7343
                     /locus_tag="KB950_000197"
     CDS             5682..7343
                     /locus_tag="KB950_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBB6856443.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            7300..7998
                     /locus_tag="KB950_000198"
     CDS             7300..7998
                     /locus_tag="KB950_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBB6856444.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            8014..9438
                     /gene="araA"
                     /locus_tag="KB950_000199"
     CDS             8014..9438
                     /gene="araA"
                     /locus_tag="KB950_000199"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBB6856445.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            9745..10905
                     /locus_tag="KB950_000200"
     CDS             9745..10905
                     /locus_tag="KB950_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBB6856446.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            11023..12882
                     /locus_tag="KB950_000201"
     CDS             11023..12882
                     /locus_tag="KB950_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6856447.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            12992..13426
                     /locus_tag="KB950_000202"
     CDS             12992..13426
                     /locus_tag="KB950_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856448.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(13685..13846)
                     /locus_tag="KB950_000203"
     CDS             complement(13685..13846)
                     /locus_tag="KB950_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856449.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(13865..14887)
                     /locus_tag="KB950_000204"
     CDS             complement(13865..14887)
                     /locus_tag="KB950_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBB6856450.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            15015..15368
                     /locus_tag="KB950_000205"
     CDS             15015..15368
                     /locus_tag="KB950_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBB6856451.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            15380..16546
                     /locus_tag="KB950_000206"
     CDS             15380..16546
                     /locus_tag="KB950_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6856452.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16638..17258)
                     /locus_tag="KB950_000207"
     CDS             complement(16638..17258)
                     /locus_tag="KB950_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBB6856453.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(17450..17746)
                     /locus_tag="KB950_000208"
     CDS             complement(17450..17746)
                     /locus_tag="KB950_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6856454.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            17950..18600
                     /locus_tag="KB950_000209"
     CDS             17950..18600
                     /locus_tag="KB950_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBB6856455.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18616..20343
                     /locus_tag="KB950_000210"
     CDS             18616..20343
                     /locus_tag="KB950_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6856456.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            20340..22232
                     /locus_tag="KB950_000211"
     CDS             20340..22232
                     /locus_tag="KB950_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6856457.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            22936..23805
                     /locus_tag="KB950_000212"
     CDS             22936..23805
                     /locus_tag="KB950_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBB6856458.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            24014..25039
                     /locus_tag="KB950_000213"
     CDS             24014..25039
                     /locus_tag="KB950_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBB6856459.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            25078..26028
                     /gene="manA"
                     /locus_tag="KB950_000214"
     CDS             25078..26028
                     /gene="manA"
                     /locus_tag="KB950_000214"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBB6856460.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            26122..27825
                     /locus_tag="KB950_000215"
     CDS             26122..27825
                     /locus_tag="KB950_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6856461.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            27827..29860
                     /locus_tag="KB950_000216"
     CDS             27827..29860
                     /locus_tag="KB950_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6856462.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            29871..30311
                     /locus_tag="KB950_000217"
     CDS             29871..30311
                     /locus_tag="KB950_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6856463.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(30511..31638)
                     /locus_tag="KB950_000218"
     CDS             complement(30511..31638)
                     /locus_tag="KB950_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856464.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(31641..32318)
                     /locus_tag="KB950_000219"
     CDS             complement(31641..32318)
                     /locus_tag="KB950_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6856465.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            32582..34348
                     /locus_tag="KB950_000220"
     CDS             32582..34348
                     /locus_tag="KB950_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBB6856466.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            34363..34800
                     /locus_tag="KB950_000221"
     CDS             34363..34800
                     /locus_tag="KB950_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6856467.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            34813..35964
                     /locus_tag="KB950_000222"
     CDS             34813..35964
                     /locus_tag="KB950_000222"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBB6856468.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(36026..36724)
                     /locus_tag="KB950_000223"
     CDS             complement(36026..36724)
                     /locus_tag="KB950_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBB6856469.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(36717..38069)
                     /locus_tag="KB950_000224"
     CDS             complement(36717..38069)
                     /locus_tag="KB950_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBB6856470.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(38292..39692)
                     /locus_tag="KB950_000225"
     CDS             complement(38292..39692)
                     /locus_tag="KB950_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBB6856471.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            39853..40590
                     /locus_tag="KB950_000226"
     CDS             39853..40590
                     /locus_tag="KB950_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBB6856472.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            40715..41194
                     /locus_tag="KB950_000227"
     CDS             40715..41194
                     /locus_tag="KB950_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBB6856473.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            41191..41958
                     /locus_tag="KB950_000228"
     CDS             41191..41958
                     /locus_tag="KB950_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856474.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            42188..42757
                     /gene="lepB"
                     /locus_tag="KB950_000229"
     CDS             42188..42757
                     /gene="lepB"
                     /locus_tag="KB950_000229"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6856475.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(42861..43463)
                     /locus_tag="KB950_000230"
     CDS             complement(42861..43463)
                     /locus_tag="KB950_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6856476.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            43569..44038
                     /locus_tag="KB950_000231"
                     /pseudo
     CDS             43569..44038
                     /locus_tag="KB950_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            44144..44728
                     /locus_tag="KB950_000232"
     CDS             44144..44728
                     /locus_tag="KB950_000232"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBB6856477.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            44731..45804
                     /gene="prfA"
                     /locus_tag="KB950_000233"
     CDS             44731..45804
                     /gene="prfA"
                     /locus_tag="KB950_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBB6856478.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            45797..46636
                     /gene="prmC"
                     /locus_tag="KB950_000234"
     CDS             45797..46636
                     /gene="prmC"
                     /locus_tag="KB950_000234"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBB6856479.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            46629..46808
                     /locus_tag="KB950_000235"
     CDS             46629..46808
                     /locus_tag="KB950_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856480.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            46830..47855
                     /locus_tag="KB950_000236"
     CDS             46830..47855
                     /locus_tag="KB950_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBB6856481.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            48046..49290
                     /locus_tag="KB950_000237"
     CDS             48046..49290
                     /locus_tag="KB950_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBB6856482.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            49443..50390
                     /locus_tag="KB950_000238"
     CDS             49443..50390
                     /locus_tag="KB950_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6856483.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            50524..51153
                     /gene="upp"
                     /locus_tag="KB950_000239"
     CDS             50524..51153
                     /gene="upp"
                     /locus_tag="KB950_000239"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBB6856484.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(51292..51534)
                     /locus_tag="KB950_000240"
     CDS             complement(51292..51534)
                     /locus_tag="KB950_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856485.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            51655..51727
                     /locus_tag="KB950_000241"
     tRNA            51655..51727
                     /locus_tag="KB950_000241"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51688..51690,aa:Thr,seq:cgt)
     gene            complement(51846..52484)
                     /locus_tag="KB950_000242"
     CDS             complement(51846..52484)
                     /locus_tag="KB950_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6856486.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        17561 a         9299 c        10868 g        14813 t
ORIGIN      
        1 attttttgag aaatattctt tgttgcgtat tgttattagg atttgtactg ctcatatttc
       61 ttttccctcc ttttattttt atctttgctg gtggcgtatg gaaacttgta tctattatct
      121 tgactatagg aaatgaaagg tagggaaagc tgttggacga agaagtgaaa gtaaaaatca
      181 gagaggattt tacggtagga gagatcagtt ctcggctgtt tggctcattt gttgagcata
      241 tgggaagtgt cgtctataca gggatttatg aaccgggcca tgaaacagct gatggtgacg
      301 ggtttagaca agatgtcctc tcttatgtaa aggaattaga attaggtgtc attcgctatc
      361 ctggaggaaa ctttacatct ggttatgatt gggtggatac tgtaggtagg aaagaacaac
      421 gaccaaaaaa aattgattta gcatggcgag gtatagaacc aaattctttt ggactccacg
      481 aattttttaa atggatcagt catgtacaag cgaaaccgat attcacggtg aatttaggaa
      541 caaaaggaat tgatgaagca aaaaatttag tagaatattg taattttcca aaaggaacct
      601 tctggagtga tttaagaata aaaaatgggc aaaaaaagcc ttttggcgtt aaattatggt
      661 gtttagggaa tgagctagac ggtccatggc aagtgggggc taagagtgct tatgaatatg
      721 gtaggttagc aaatgaagcc tcaaaagtca tgaagttagt ggatgattca attgaaacaa
      781 tcttagttgg aagctctacg ccacgtttac catcgtaccc tgaatgggat cgtatcgcat
      841 tagaagaagc ttatgagaat gtagattatt tagctttgca taactatatt gataagtatg
      901 atgaagaaga cttgactaaa cctcctaaaa atgagcgtga tgatacagcg acataccttg
      961 caaaagctta tcgattcgat cgtcaaattg aagaaatcgt tgctacttgt gattatgtaa
     1021 aaggatattt gagaagtgag aaaacaatgt atctagcgtt tgatgaatgg aatgtacatg
     1081 cccaaccgga aaaaagacat agggattttg aagtcggtag tcccatcgat tggtgttact
     1141 ttactatgga agatacatta ttatttggtt cattagggct ggctatttta cgacacgcag
     1201 atcgcgtaaa gattagttgt caatcattgt tagtaaacac aataccttta atactgacag
     1261 atgctaaagg acaagcatgg aggaatccaa cttattatgt gatgcaacac ttatcgaaat
     1321 atgctaaagg tcaagtattg aaacaacaaa tcatttgccc tgtttatgat tgtgaaaaga
     1381 taaaaaatgt tccttatgtg gacagtgtga tcgtagataa cggcaatcag ctgactattt
     1441 tcttgatcaa tcgcacaaat gaacaacaaa agataacgat tgaacatgat ttcaatttgc
     1501 tagatcaagg agagcactac acattaacca gtagcgatct gttggacaaa aatacacgtg
     1561 aagaaccaga aaaaataaaa cctgaaataa actatttttc aatagaaaaa catgacgaat
     1621 taaatgtagg gatagaaaaa tactcttgga atgtaattag aataaagaaa atgtgaggaa
     1681 atttgttatg atgaagacac tttataataa tcctatcgtt cggcaacggg cagatccttg
     1741 ggtatataaa catacagatg gttattatta ttttacagga tcggttccag gctaccaagt
     1801 gattgaatta agaagggcaa agtcactgaa tgaattagaa catgctgcaa gcctgattgt
     1861 atggcaggct catgaagaag gaccgatgag tgaattgatc tgggcacctg aagtccatta
     1921 tatcaatgga aaatggtata tttactttgc tgcaagcaat cataaaacta tacgtgatga
     1981 atctcatcat caccgcatgt tcgtacttga aaatcagcat atagatccaa tgaatacaga
     2041 ttggatagaa aagggacaaa taaaaacgaa aagggaaact ttcagtttag acggaacaac
     2101 tttctcttct caaggaaagc tatattatgt gtgggcccaa caagatcctg aaattactgg
     2161 gaattctaat ctttatctat ccgaaatgga taatccttgg acgttaaagg ggaagcagat
     2221 cctcctttct atacctgagt atgaatggga aaaacggggt ttttcagtaa atgaaggtcc
     2281 agcagtcctt actcgcaatg ggaaaatatt cattacgtat tctggtagtg caacagatga
     2341 aaactatgcc atgggcctgc tgtgggctga tgagcacaag aatcttttat taaaagaatc
     2401 atggcataaa ttatcggagc cagtattcgt ttcttctaaa aaaaataagc aatttggtcc
     2461 tggtcacaac tcgtttacgg tttcagaaga cgggtgcttt gatattttga tctatcatgc
     2521 tcgaccagag aagaaccaaa aaggggatcc gttagacaat cctaatcggc acgcgaacgc
     2581 gcaatgcttt acgtggaatg agcaaggatt tccagaattt ggtgaacctg ttccgtatgc
     2641 cttatgagca caagaaacct tgatttttgc ttgaataaac cttgcagact cgttatgata
     2701 tatgtacgta caaataaaaa ggggcaaggc aaatggcaaa atatcaagat attgcagaag
     2761 agataaaaca aaagatcctt cgtgaggaat acgaagtaaa tacaaccatt ccctcagaat
     2821 tcaagctgca agaaatatat gacgtcagca gacatacgat tcggcaagca atcgctgtgt
     2881 tggtaaatga gggctatctg cgtaaagaaa aagggtcggg cacatatgta gatgacagct
     2941 ataagaaaga aaaaacacca ttgaacagaa caaaaacgat cggtgtcatc accacatatc
     3001 tctctgatta tatttttccg tcgattatcc gaggaatcga aaatgaatta cgagaatctg
     3061 ggtattcatt gcttttagcc agtacgaata acgatcacga attggagaaa gagtgtttga
     3121 agaaaatgat tgcttatggg gtagatggtt tgatcgttga gcctacgaaa agtaatcagt
     3181 ataacccaaa cttagcactt tatgttgctt tgagagaaca aggcattccg atggtgatga
     3241 tcaatgctgc ttatgaagag ttaaatgcct cttatatttg tttagatgat gtgaaagtcg
     3301 gttttatggc agcagaatat ttgataaaaa aaggacatca gcgtctgttg ttcatcacaa
     3361 aaatcgacga tcttcaagga aaatacagga tgaaaggttt tatcagcgcg tgtgaacagt
     3421 ataatgtgcg ttttttgacg gaagatatca ttactttcac cacggaaaca cgagaaacaa
     3481 tcgctgaaaa aacagtacaa cggttgaaag aagaacaagc tttagatggc atcatttgct
     3541 ataacgatca aatagctagt ttaatgctag ataagttgac agaaaatggc tatcatattc
     3601 cagatgacct atcgatcgta ggaacagacg attcttcttt aagccattta gggaataaaa
     3661 aattgacaac attgacgcat ccaaaagaaa agttgggaca agatgccgca cggtggatcg
     3721 tcacaacgat tgaaaaagga caggcacagc caaatattct ctatgaacct gtactagttg
     3781 aacgggaaac agtgaaagaa aacgaaaaat aagagacggg tttttaccgc ctcttattgc
     3841 tcctacggtt actcgtcgca atgtttgtga gagcttttgt catcgctcaa ggctgaacgt
     3901 ttctttcacc acctctccac taaacggtgt tctcctacca actggtcaca agtcacaata
     3961 ttcatcaaac atgagaagct gttttttgaa attgttcctt gtgactggaa actaagcggt
     4021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttaagaa tgatggatag
     4081 aaatacgttt aggataagtt tttttagatg cactttgttc gatttcctct aaagatttcc
     4141 cacgagtttc tggtacgatc tcttgaataa ataaaacccc taaaaagcag atgatcccaa
     4201 aaatgccgaa aacgatttct tgagacatag aagcagtcat cacaggaaaa agcaagccaa
     4261 ctaggaaaga accaatccaa ttaaaagaag aggctaggcc agatgctcgc ccgcggacag
     4321 ctagtgggaa tatttcgcct acgattaccc atgtcagtgg agcccatgtg caagaataaa
     4381 atgcgacata aatacataaa aacaagagaa tcaacaacga atttgtatga gcatccagta
     4441 cagttcctaa aacagctggt aggatgaaag aaagtcccat cacgctaccg cctattttca
     4501 ataaagttcg ccgattgaac ttgtctgcaa taaccaaaaa gagcaaagaa ccagcaacta
     4561 aaatgattcc ttggatgatc ggccacatca atgcatcgct cgctgcatgc ccagtcgctt
     4621 tttctacgat cagcggaata tagtaaaaaa tagcattagc tccttgaaat tgctggaata
     4681 ctgctacacc aataccggca atgactaagg aacgatattt ttctaaaaac aaagtatgcc
     4741 aagaagttcc ggttgtcttt tcttgtttga cattcagctg gatttgttcg aattcctgtt
     4801 cagcttcttt tggtgtccga atatagttca atactctttt tgcctcttcc aagcggccag
     4861 attgcataag aaaacgcggt gactctggta gagctaaaac accgaaaaat aaaatcagcg
     4921 cggggacagc agcgagccct aacatcacgc gccaagccat cgtttcaggc aaccctttta
     4981 gcaaataatc tgcaacatag gaaaggagca tgccagaagc aatcatcact tgattgatcc
     5041 ctgacaatct tccccttagt ctagcaggag ccatttcaga catataagca ggaactaggg
     5101 cagatgcagc gccaacagct aaacctagaa gaatccttgc tgcaatcaga aaataagaac
     5161 cattgtgagg ggcagatgca gaaaggacag aaccagccat aaaaataagt gcagaaagaa
     5221 gaatcatttt tcgtcgtcca agcttatcag aaagaagacc agataaagag ccgccaagga
     5281 tcgcacctaa catgaccgaa gacgtgatcc atcctatcaa agaagcttta cctgccaaac
     5341 cccaatcatg ctgtaaaaaa ggtaaggcgc cggtcataac accaatatca tatccaaata
     5401 gaatgccgcc aaatgcgcca aaaaaataaa taaacgatga ggaaactttt ttttcttttt
     5461 tcataagtat tctctccttc tagaatttcg agaaggcggt tgatcagatt ttgtaagcaa
     5521 tttcatttta taacttatgt acgtacaaaa caagtgtttt ttattgtatt cttgtaaata
     5581 actgcttgac atgtacgtac aaaagtatac aataatcatt gtaagcgcta ctaaaaagac
     5641 ggtttgatca ggtttgacga gaaggaggga gttaaatcat tatgaaggaa gtaaatgcgc
     5701 aaagtatgtt agatgtcata gatggtcgta catctttagg gattgagttt ggatcaacta
     5761 ggatcaaagc tgtattgatt gattcatctt ttactcctat tgccgctggt agttatgagt
     5821 gggaaaacaa actagaaaat ggtatctgga cctattcatt agaagatatt tggaaaggac
     5881 tgcgcgtcag ttaccgaaaa atggcagcag aagtattcga aatctatggc gagaatctta
     5941 cgacaatcgg gtcgattggg ttcagtgcaa tgatgcacgg ttatctagca tttgatagca
     6001 aggaacagtt actagtacca tttcgtactt ggcgaaatac aatcactgaa gaagcagaac
     6061 gaatattaac ggaaaaattt caatacaata ttccgcagag atggagtatc gctcatttgt
     6121 atcaatctat tttgaataaa gaagaacatg tgaaagaaat taattttttg acaacgcttg
     6181 caggttatgt ccattggcag cttactggtg agaaagtact aggcgtagga gatgcatcag
     6241 gcatgtttcc tatcgatcct tcaacgaaaa cctaccatca agaaatgaaa aatatttttg
     6301 gtcagttaat cgaaaaagcg cacgtagcga ttgacttgga tcacattttg cctaaagttc
     6361 tagtagcagg agaagaagct ggcaagttaa gcgaatcagg ggcttatctg atcgacccta
     6421 cgggaaattt gagaccagga atcccggttt gtccgccaga aggggatgca ggaaccggta
     6481 tggtcgctac gaacagtgta gccaaaggga cagggaacat ttcggctggt acttccgcct
     6541 tcgctatgat cgtattagaa aaagaactag aaaatgtcca tccggaaatc gacatggtca
     6601 ctacaccttc aggagaacta gtaggcatgg ttcatacgaa taactgctct tcggatatca
     6661 atgcttgggt cagtattttt gaagaattta ccaattcttt gggtatggag ataagcagag
     6721 ataaattatt cacggtatta ttcaacaaag cgctagaagg cgatacggat tgcgggggat
     6781 tactttctta tggttatttt tccggtgaaa acattacagg agtaaatgaa ggaaggccat
     6841 tatttgtacg tacaccaggt agccgttttg atttagcgaa tttcatgcgg atccatttag
     6901 ccagtgcttt tggtgcaatg agaatcggaa tggatatatt gaaatctgag aatgtccaaa
     6961 ttgatcgttt agtggggcat ggggggattt ttaaaacacc agaagttgga cagcggattc
     7021 tagcatctgc tatggaggca ccagttacag tgatggacac agccggagaa ggtggagcgt
     7081 ggggaatcgc gttattagca gcctatatga tggatcgaca tgggaagtct ttagaaaact
     7141 tcttggcaga agacgtgttt ggacaagacc aaggaatcac catcacacct tcagcagaag
     7201 aaatagcagg ctatcagata tttatgagac gttaccgtga ggggatcgac attgaaaaaa
     7261 cagcgattca tcaattgcaa gaaaaaggag tgatagaaaa tgctggaaca actgaaagaa
     7321 gaagtatacc aagcaaatct tgacttgcct aaacacggac tcgtcaaata tacatgggga
     7381 aatgtgagcg cttttgatcc tgaaacacga tatttcgtta tcaaacctag cggagtcagt
     7441 tatgaagaga tgacagcaga cgacatggtc gttgtggact tggacaatca tatcattgaa
     7501 ggtaaactca atccctcttc ggatacgcct actcatgctg ttttatatcg ttcatttcct
     7561 caaatcggag gaatcgtcca tactcactcg acatgggcga cgatctgggc acaagcagga
     7621 ttggatgttc ctgcgatggg gacgacgcat gcagatactt tttatggatc ggtaccttgt
     7681 gctcgatttt tgacacaaca ggaaatcgac agcgggtacg aatatgaaac aggaaaagtt
     7741 atcatagaaa cattcaaaga acgcaaaatt gatcccttag caattccagg ggtactttta
     7801 cacggacacg gtccatttac ttgggggaaa gatgcgcaaa gtgcggtaat gaacgcagtc
     7861 gtactagatg aagtatgcaa gatgaattta tttaccagac agctcaattc attttcagaa
     7921 gagctgcctc aacggatttt agataaacat tatttacgaa aacacggaaa aaatgcgtat
     7981 tacggacaaa acaaataaaa ggagtggcta attatgttga atattggaga aaaagaattt
     8041 tggtttgttg taggatcaca gcatttatat ggggaagaag cactgcgaga agtaaagaaa
     8101 catgcacaag aaatggtcga tgaactgaat gaaaatggcc aattgcctta tccaatccgc
     8161 cttcaagaat tagcagttac tgctgatacg atcacaaaaa tcatgaaaga ggtcaattat
     8221 cgagaagaag tagcaggtgt gatcacttgg atgcatactt tctcaccagc aaaaatgtgg
     8281 atccgcggga caaagcttct acaaaaacca ttgcttcatt tagctacgca atataacgaa
     8341 agcattcctt ggaaaacgat cgatatggat ttcatgaatt taaatcaatc cgctcatggt
     8401 gaccgcgaat atgggttcat caatgctcga ttaaataaac aaaataaaat cgtcgttggc
     8461 tactggaaac gacctgagat ccaaaaagag attgctgatt ggatggatgt tgcagtagcc
     8521 tataatgaaa gttttggaat aaaagtggct cgttttggtg acaacatgag aaacgtgggt
     8581 gtcacagaag gagacaaagt ggaagcacag attcaatttg gttggactgt tgattatttt
     8641 ggtatcggag atcttgtaca agtaatcgat cgcgtatcag acgaagaagt cgatcaatta
     8701 tttgaagagt acaaggaatt gtataccttc gactatggtg attatgaaga aaagacatgg
     8761 gaggaacatg taaaagtaca agctcagcag gaaatcggta ttcgccgttt cttagaagaa
     8821 ggaggctata acgcttttac gactaatttt gaagacctgt atggaatgaa acaactacct
     8881 ggactagctg tacaacgttt gatggcagaa ggatacggtt ttgctgggga aggcgactgg
     8941 aaaactgcag cgattgatcg gttgctgaaa atcatggctc gagggaaaga tacaggattc
     9001 atggaagatt atacttacga gctagctagt ggccaagaag caatcttgga atcccatatg
     9061 atggaagtag atccgacgct tgctgcgacg aaaccaagaa tcgttgtttc tcctttatca
     9121 atgggagata gagaagatcc ggcgcgtctt gtatttgatg ggaaagcagg agaaggggta
     9181 gtcgtatcga tggcagattt cggtacgcat tataaactgc tgatcaatga agtagaagcc
     9241 tttgaaccaa cgactgaagc acctaattta cctgttgcac gtgtgttatg gaaaactaaa
     9301 ccgaatttcc atgaaggtgt ccattcatgg attcaggcag gtggcggtca tcataccgtt
     9361 gtctcgctaa atctaacaac agatcaaatt gaaacgtggg caaaattagt tgagttggaa
     9421 acagtggtta ttagataaaa agaggttgtg aaaaagccac ttagttttaa gaaataagaa
     9481 gagattgacg aaatttgctt ttcaactttt ttgaaattta gtcttattat caatgtcagc
     9541 taacggaaca ccatttatca agatatctca ccctcaatat tttgctctcg aaaataaggc
     9601 ttatggcagt aacttgtcat agccttattt ttatcactca gcctactctg ctctaactgc
     9661 tctttttgct tctaggaagt caaatggtaa acagttgtct aattgcagaa atttcgagat
     9721 acttgttagt aaggaggggg ttttatgtat cacgttcatt tgattgatgc tattccagaa
     9781 caagagatag aaaagttaca aggggaatat ttcaaaataa tagatgattt gccagccgaa
     9841 ggaatagtta ttcgtagtac gaaagtgaaa gatgaatggc ttacaccaga tttgctagca
     9901 atttcaagag ctggtgttgg ggtgaacacg atcaacgttg aaaaagcttc tgaaaacggg
     9961 acgatcgtca tgaatacacc aggtgtaaat gctaatgcag taaaagagct tgttttgtgc
    10021 tgtttgctct tatcttcccg tccgataatc gaagcaagtc gtatggtaca aacactaaca
    10081 ggtcctaata ttttagaaca ggcagaaaac aaaagaagtg cttatgtggg acgagaattg
    10141 caaggcaaaa ccatcggtct tcttggtctt ggagcgatag gcacaaaagt agcgctaagt
    10201 tgctatagct tagggatgga tgtcttgggt tattctatcc gcgatgccca gttggattat
    10261 gttcggcaag cagatttaga aacagttctt tctacctcgg attacatcgt agtcatgctg
    10321 ccgttgacag aagatacaaa aggattgatc gatcaggcga atatagaaaa aatgaaaaaa
    10381 gatgctgtac ttatcaatgt aggaagaagt gaaattgttg ataaatatgc tgttatgcaa
    10441 gcattggaaa aacaacatct ggctaaatac ttgactgatt ttccagaaga agagttctta
    10501 gaaaatgacc gtatacttat gttgcctcat ctagggggat cgactcagga agctttggca
    10561 gacagcggac gtttggcagt tgaagcctta aaagattatc ttttgttcgg tacggttaga
    10621 gaagcggtca attatccatc cgcacgaatg tttttccaag cgccgtttcg attcactata
    10681 ttttatcaaa agaaaaccaa tatattagca gagatatttt ctctgctgaa tgagaacgag
    10741 ctagcgatcg cagatatatc gcgtaaccat aagaatgagc atgtctacat attgattgac
    10801 atagacagta ctgattttga acagctcaat caagtgaaac aacagctaga aaaaatttct
    10861 ggagtacgaa aggtccgctt attaaagaaa ccagaaaggc cataaagaaa gagaatattt
    10921 ggatttcatt aattcttctt tccgttcttt actttcgaat ttcgtacaac taaaataaga
    10981 gaagcaacat tcttttattg atagaaatga aggagcaagc atttgaaaag atatataagc
    11041 agactttcga ccaaacagaa aaagatttta aaaggaacag cagcaatcat cagtatcttt
    11101 tttcttgttg tttttagtaa tttatttttg cagtggtgtc aaaatgatct atccgtagac
    11161 cttgcgttga aatttgcttt ttcttggcat acggaaaaat tcttcttagc ctgtctcgtc
    11221 ttgctgatta ttcttatttt tctgattgca ctagcaggat ctgtcccttt aggttccctt
    11281 acgtatgtcg tagccatcgg tgtattaggt tttgcaaatt atatgaaaat gagttatcgg
    11341 caagaaccga tttatccaga cgatttgaag atgatcactg aaatcggatt gctgaaagat
    11401 atgaccggca ctatgctgtt tacagtgatc ttagctgccg caggaaccgt gataggattg
    11461 ttttgctggt acatgtttcg aagcctgaaa aaaggaaggc gattccaatt gatccgatta
    11521 acgacactgc ttgtggcaat tggactgcta gggtatatca gtaatttcaa caatcccgat
    11581 aatttgttgc gaaaagcata taataaaacg gcactatgga taccttacag ccagaaaatg
    11641 aactactata acactggttt tatcggtggg tttttgtaca atctgaaggt agaaccaatg
    11701 gatgaacctg aaggatattc gaaagaaaag atcaaagaaa tcactgaaaa ataccagaag
    11761 ctagccgatg aaaaaaataa aactgtagag gaagagtcgc cgaacatcgt gttcgtgatg
    11821 agtgaaagtt tctcagatcc tagtcgatta aacggcgtag aagtatctgg tgagccattg
    11881 gcagattatt atgaagtagc tgatcaaaca tatagtggca atatgctttc tcaaaactat
    11941 ggcggtggaa cagcaaatat cgaatttgaa gcattgactg gtttttctat ggcgttattt
    12001 aatgcgcaac tcaccacccc ttacacgatg cttgtaccaa aaatggatca gcttccttct
    12061 atcgtttcag cgttaaacgc acaatcatac cagacgacag cgatccatcc atacaatact
    12121 tcgatgtata aaagagagga tgtttatcag acattaggat ttgaccagtt catcagcgag
    12181 cgtacgatga cctatacgga tacgatagag aataatccat atatttctga tgagtcagct
    12241 tacaaagaag taatgacact tctcaaggaa gaaaaaacac ctcagttcat tcatttagtc
    12301 acgatgcaga cacatatgcc ctacgatgga aagtatgata atctagacta tcctgcaaaa
    12361 acttcagatg gaagtgggac acttgactta gagaattatc tgcaagatat ttcctatagc
    12421 agcacggcac tgaagcagtt cacggaagaa ttgaagaatc tttctagaag gactttagtt
    12481 gttttttggg gggatcatct tccagggatc tattcagata cgatccaagc aaaaaatgac
    12541 aaacaaacat tgcatgaaac acaatttctt atgttcgaca gtaaaggaga attagaaaag
    12601 caaacgactc acgatgcaat caccagtccg ttttattttg cagcgaatct aatggaacag
    12661 acgaatcaga caacaaatgg cttttatcag ctgcttttat cattagaaca ggaacttccg
    12721 gcgtttgaac gagaactgta ctatcaaaac ggacagtggt ataaagaagc gcaattcaat
    12781 cgctcgcaac aagagattta cgacgaatat caattgatcc aatatgacat tgtcgcaggg
    12841 aaacaatata gtttagcaga tggtttcttt gagcacgaat aagaatggcg agctgtatag
    12901 aaaccattaa attttttcaa gtacttgtca tttgaggata aaaacaagta gaataaataa
    12961 taacaacaat ggagaaagta ggaaaacgaa catgttaccg aatattggct attatgtgca
    13021 gtccatcgat caaatcgtca aggaaacaga agcaatcgga gaaggaatgc atccgaaata
    13081 tgaagtgatc agaaacgcaa ttgatgaagg aaaaacaagt gaactggaca aatcgactct
    13141 tgaagagatc atcctgttat ttgaagaagg aacaaaaaaa tatcgggcaa tgcttatgaa
    13201 agtcagtact ttgcgtccac cagcaaaagc aatgggcatt cataaaaatt ttgaaaaagc
    13261 ttatctttca tatgtagcag gctgtgaaga aatgatccaa agtatcgatc ttgaatcagg
    13321 ggtagatgaa gatctattca atacatctga agcaaaacaa gaccaagcga cagaccaatt
    13381 gacagctgcc atccaaaaaa tgactaattt gttgttgaaa aaataaaaga aatctttgat
    13441 aacaataaaa actagagtta tctaatataa atgtaatatt acctttatga tagagtgatc
    13501 aaaatccttt atgataatga catacctata accaacaaca accttgtact tactcaaaat
    13561 cgagtaaata ctccttttcc gtcgagtcta accatctcgg cggtttttta tttgtagaaa
    13621 agaacatcag gatagacgga aaagtcttgt cctgatgttc ttttcagata gtatggaaag
    13681 ctgattagtt ttctacattc tgtacatatt gttcaaagta gttctgtaaa tcggctttga
    13741 gtttctgata ttcctcttcg ctatactctt tatcttcgat tttcccatca aagaaaggaa
    13801 tatttagttc ttcccgtagt ctgtctaata cgtcttgctg attcatttca ttctcctttt
    13861 cttactaacg atcaaaataa acacagacac attctggtac atagacatct tttttcagta
    13921 attccagtaa tcctgtttca ggatgacggc gatataatgt cagattatcg gtgttctgat
    13981 tggcacacac gacaaaatta ttgcttggat ctagtgcgaa atcgcgggga aaatcccctt
    14041 ctgttgagat gatttgcttg ttttgaatgt gggaaccatc ttcaaaaact gcgaatacag
    14101 caatcgagtt atgtcctcgg ttagaagcat aaaggaactt gccatcattt gaaatgcgga
    14161 tagctgcccc tccattttca ccgtcgaatt cttctggcaa ggtcgatatt ttctgtaatt
    14221 cttcaaaagc acctgtttct ttattataag aaagcacgcg gactgtgctg tctaattcac
    14281 caaataaata agcgaattgt ccatttggat gaaagacaag atggcgaggg cctgttcctg
    14341 gctctgcagt gaaggtagtt acaagattca atttgccgtt ttcggacaca tcataagtat
    14401 acacacgatc tgtaccaaga tcacatacga ctaatcgttg gtcaggagtc agatccgtat
    14461 agtggacatg cgcatgatct tggtttttat gaggacctgt cgcttcttga tggaatattg
    14521 aatctgctgc tttcagttcg ccatctccta atatatggta gacatttact tcacctttat
    14581 gatagttcgc cccgtatagt aattgacgtg cttcatcaac cgctacataa cacaaaggag
    14641 cgccagactc tgttaccgca ttcaaaaaat gaaaatcctt atcgtacgca ccggcaccac
    14701 ctaatccatc cacactagta actgtgtaaa gattgccagc tttacttttt gctacatagg
    14761 tagggctatt ttcttcaaca gctaatgaaa ggttggctaa ttcttctttt tctgtatcaa
    14821 ggtcgatcgt atagattcct ttactttcgc gacgcgtata ggttcctaaa attatctttt
    14881 cgatcatatg agctgattcc tcctaattgt aatgaaatag ttttcgtttt tattctatca
    14941 cataattatt ccacacggct aaagaatgag aatgtgttat actgtttttg aaagcaatta
    15001 aaggagagag tgcaatgatt caggcaaaga ttacagaaat tgggacagaa gcaataaaca
    15061 aggaagaacc catgctgatt ttgttcgata aaacagcaac agctgcctta cgaaaatatt
    15121 cggtgattca agaagtatca aaagacgaac ctttttcttt gaaagcaggc ggaacaattg
    15181 cttttgatga tcaggtatat acgatcgatt atgtcggtcc gatggccaat gggaatttaa
    15241 agacagtagc tcacgtatca ttgatttttg atgcttgtcc agaagaagga caaattgcaa
    15301 atggaatcta tcttcatccg catagactgc ctgctattgg tttgggaagc caaatttttt
    15361 ataactaaca ggaggaatta tggaaggaaa aaataagaag aacagaccgg tatcgtggtt
    15421 ttggcgctgg tttttaaata atcaagtagt cacagcctta cttgttgtat tgttaatatt
    15481 gctgatcgtc tttttattta caaaagtttc atatttgttt gcaccaatct ggcaattttt
    15541 agcaatcgta ggtctgccta tcattttagc aggaatcttg tattacttga tgaatccggt
    15601 agttgattac tttgaaaagc gaaaagtacc aaggctttac agtatcattg ggctgtttgt
    15661 tttggttgtt gcactaatcg catgggggag cgtagtgatc atccctaaga ttcaggaaca
    15721 aacagtgagc ttcatcagta acttccctca atatgtagat acgattgata ataaattaac
    15781 cgagattttg cgggacccgc tgttcaacca atttcgcgaa cagttggaaa cagcaggtga
    15841 taagttcatg aattcggcag gcgatatgat ccaagatata tctaagagta cagttcagag
    15901 tcttggaagt tttgtcggcg cggtagcgac tattttagtg gctctgctga cgatgccgtt
    15961 tatcttattt tatctattga aagacggtaa acaattagca ccttattttg tcaaattttt
    16021 accgacacgc atgcaaaaac cgacgttgaa tgtgctgagt gaaatgaata gtcaagtttc
    16081 ttcttatata agaggacaac taactgtggc ttttgctgtg gcaatcatgt ttatgattgg
    16141 ttttgcggtc attggtttag agtatgctgt cacgttgggg attattgcgg gttttttaaa
    16201 cctgatccct tacttaggat catttttagc gatgattccg gctatctttt tagccattgt
    16261 agcaggtcca ttcatgatca tcaaagtact gatcgttttt gcagtagagc agacaataga
    16321 aggacgcttg atctctccat tagtactagg gaatcagtta gcaatccatc cagtaacgat
    16381 tttattagtt ctgctgactt ctgggaaatt gttcggaatc atcggtgtga tcttggggat
    16441 tcctgtttat gcagcagcta aagtagtgat tactcatgtt tttgaatggt acacaacaat
    16501 ctcttctctt tatgaagaaa atccgttgaa aaagcagaaa gaataacaaa aagagacaga
    16561 tgggaaaata tccaaggcac aaatgctgct ttgaagattt tttcctctgt ctcttttgta
    16621 tgcatttatc tcaattttta aaatgattta cctaaagcag cagctttatc taatgcattt
    16681 tgcatcaact catctgcacg gtcagggtca taatcgattc cttcgatgaa tagctgatct
    16741 acttgatcga tgcctacaaa gtttaaaata ccttttacat attgagaggc aaagtcttgt
    16801 ccgttgtata caccgccatt agattggatg tgcagtgctt ttttacctgt cactaatcct
    16861 tctggcccgt tttctgtata gcggaacgtt tttccagcta cattgacggt atcgaaccaa
    16921 gcttttaatt ttgttgggat atttagattc cataaggcgt ttgcgataac gattttatca
    16981 gcagcaagga actgatcagt caattcattg aaacgagcaa ttttttgttg ttgactttct
    17041 gataaggaag tgaattctgc accagcacgt aatgcttccc aacctgataa taattcttca
    17101 tcgatttccg gaatatcatc gcgataaagt tccaaaacag tcacttcatc atctggatga
    17161 ttttctttat aacttgttaa aaaagacgtc aatgccttaa ctgtgcgcga ttcttctgca
    17221 gttaggggat gacctttgac tactaaaaca gatgccatat gcttaccttc tccttttatt
    17281 gctatgattg ttataactat gactaacaaa atcagtttac tcgaaaaaga aaaggctatc
    17341 aagtcatatt gataaggttg aagaataaat aactaaaaaa agacagggta taaaagaggc
    17401 tgtgacacga ttttttgtca cagcctcttt tgaatctaac aagcaatatt caagcaacac
    17461 tcgactgaag agcttccatc ccaccaatga tataaacagc acgaaaacca ttagcagtaa
    17521 gttgatcggc taatgcttct gatcgcctgc ctgtatagct tagaatataa taaagtttct
    17581 ttggatctag attttttaat tgtcttggca agatagcagc ggggatagaa agcgagtggc
    17641 ttaagtggtt ttttgtgtaa tctaaggtat ttcttgtatc taaaacgatg ctgtcagcat
    17701 cgagaggcaa tttattgaag tcttggacac taattgattg ggccatttat ctcatccttt
    17761 gtttagtatg tggctatttt aatgggcaac tctcattaat tcaaatggaa ggattcctct
    17821 tatcactttt ttaatgtatc ggatgataat gaaaaaaact atgcaaatgt cttgcaaaac
    17881 aggtaggagg tgttataatg acatagcgtc atatgacaat gtgtcatctt gaaagaaagg
    17941 agaatccata tgccgacaca gacatttttc catttgccta aagaaaaaca aaaaagacta
    18001 atcgaggctg cgagaatcga gttttctaga gtacccttaa aagaagcttc gattgctaat
    18061 atcgtcaaat tagcggatat ccctagaggc agtttctatc agtattttga agataaagag
    18121 gatctttatt tttattactt tgagacaatg agacgtgaca gctctaaaga tatgttacaa
    18181 ctaatgaaag aatccgatgg tgatctattc aaaggattcg aatcctattt ttctaaaatg
    18241 atttatgaga ttttggaagg cgagaatgct tctttttatc gtaacttgtt tatgaacatg
    18301 gattaccgtg cctcaagaaa agtcgctcct catacgagtc gccaaaaaac gcctcacgat
    18361 ccacataaag aacacaaaca agatgcaaaa gagttgcttg aattgatcga tcggacgaag
    18421 ctgaaagtaa aaaatgatca tgaattggaa ttactgatcc aattagtgat gaatactgta
    18481 tttacaacaa ttgcgcattc gtatcgctta ttttcaacat ctgaagatta ctcagtagag
    18541 caagcaatca gtgaattcaa tttgaaactt tcttggctga aaaacggagt atataaatag
    18601 aaagaaggga actagatgct aaaaatcgta aaacggatct ccttgacttc agcaattgca
    18661 gctgcagttt tcatggtgat ccaagtactg gcagatttat atttgccgac acttacctcc
    18721 aatattattg ataaaggggt agcgcaagga gatgtcgatt atatttggca tacaggtttt
    18781 gtgatgatcg gcttttcttt gatcagtatc ttagctgcta tcgcaaatac attttttgct
    18841 actcgtgaat cacagaaact gggtaagcaa ttgcgtacgg atgtctataa aaaatcagag
    18901 agtctgacaa aagatgcgtt tgataaatat ggaacggcct ctttgatcac aagaacgacc
    18961 aatgatgtga ctcaaatcca aatggttaca caaatgtttt tacgtatgat gatcaatgca
    19021 ccaatcacgt tgattggcgc gagcatactt gcttatcaaa aagatcatca gttgacaaaa
    19081 atcttcttag ttgtcatccc agtaatgatt attttgatcg gcggaatcat gtactttgct
    19141 gttccattat tcaaaagtat gcaaaagaaa actgaccgtt taaacctggt atttcgagaa
    19201 ggattgacag gggtcagagt gattcgagca ttcgataaga cacgctttga agaaaatcgt
    19261 tttgatttag cgaataaaga ttatacaaat acggcaatca aagttaatac gatcgtcgca
    19321 ttgatgatgc caatgatgac attgatcatg agcggtacaa acgtagcaat cacttggttt
    19381 ggtggacact acatagcaga tatgactttg gaagtcggta atctgattgc atttatgact
    19441 tatgcgatgc agatcttgat cagctttatg atgctttccg ctatttttat catggtgcct
    19501 cgtgcacaag cttcagctga ccgtattaat gaagtgttgg atgaaaaaat cggtatccat
    19561 gaccctgaaa atcctaaaac agtttctttt gctggaaaaa acgcaacatt ggccttcaat
    19621 catgtcaatt atcgatatca tggagcagaa aaacttgcat tggaagatat tgatttccaa
    19681 gccaagtcgg gcgagatcgt cgcaatcata ggggggactg gttcaggtaa aacgacctta
    19741 gtcaatttga tccctcgatt atatgacatc gaatctggtt cgatccaaat aaatggaacg
    19801 gacattagtg acatgactca atataatttg cgagaattaa tgggctttgt tcctcaaaaa
    19861 gcagttctgt tctctggaac gatccgagac aatatgcagt atggtaagcc ggatgcaaca
    19921 gatgaaatga tttggaaagc attagaaatc gcacaagcta aagattttgt ttcagagatg
    19981 gaagatggct tagatagcca tgtggaacaa ggaggaggaa acttctctgg tggacaaaga
    20041 cagcgtctgg caattgcacg ggcattagtc aaaacagcag atatctatgt gtttgatgat
    20101 tccttctcag cgttggattt caaaacggat gccaatcttc gccaagcatt gaaaacgaat
    20161 atgaaagaaa gtatcaccgt tttagttgct caacgagtaa gtacagtaat ggatgcagat
    20221 atgatccttg tgctagatga aggaaaactg gttggcaaag gaacacatga agaactatta
    20281 gcaacaaacg aaacttatca agaaatcgtt cattcacaac taagagagga ggacttagca
    20341 tgagtaaaac aaacacacgt ccctctcatg gaccagcagg cggtccagga catggtggac
    20401 ctggcagaaa tctcggtgta aaaggtgaaa agcctaaaaa tttctgggga acggtacgcc
    20461 gattgtttgg ctatatgtct aaacgtatgg ttgcgatcat cgctgtattt gtactagcaa
    20521 tagcagccgt gatcttccaa atccaaacac caaaggtctt ggggaaagca accacagaaa
    20581 tattcaaagg tgtaatggct ggcgctcaac agatgcaaca aggccaacaa gtaacaaaat
    20641 ttccgataga ctttgataaa atcgcgcaga tcattgcgac agtaatcgtg atgtatttga
    20701 tttctgctgt attcaacttt ttacagcaat ttattatgac acgtgtttcc caacgaacag
    20761 tttatgaatt acgtcatgat ttggaagcca aaatgaatcg tgtaccgatt tcctattatg
    20821 atacacatac aaatggggat atcatgtctc gagcaatcaa tgatatggat aacatcgcaa
    20881 gcacgctgca gcaaaatttg actcaatttg taactagtgc agtcacattg attggggtca
    20941 tcgtgatgat gctttcaatc agctggcaat tgacgttagt cgcgttattg atgatccctt
    21001 taagcttgat catcgtgatg atcgtagcgc cgaaatcaca agtgtttttc gcagatcagc
    21061 aaaaaagttt aggtttattg aataaccaag tagaagaaac atatggtggt cataccattg
    21121 ttaaaacatt caatcatact gaaaaagacc aggaagtatt tgaaaaagaa aatcagaatc
    21181 tgtatgccgc tggttggaaa gctcaattta tttcggcaat catcatgccg ttgatgaatt
    21241 tcgttaaaaa tctaggctat gtgttcgtcg ctgtactagg cggagtaaaa gttgcaaatg
    21301 ggaacatgac attaggggat gttcaagcgt tcttgcaata tacaacacag ttctctcaac
    21361 caatcactca acttgcaagt ttgatgaaca ctatccaatc aacagttgct tcagccgaac
    21421 gggtatttga agtgttggat gaagaagaga tgtccaatga aaaatctggc cttccagaag
    21481 aaaaagatac accatacaaa gttcgttttg aaaatgttca attcggctac acaccagata
    21541 acttgctaat gactgatttt aacttggatg tcaaacctgg tgagatggtc gctattgtag
    21601 gtccgactgg tgcagggaag acgacactga tcaaccttct tgaacggttc tacgatgttt
    21661 ctggaggtag tattcgttac gatggtgtag atacccgtga tctttcacgt gatgaattgc
    21721 gcgcacagtt ttcaatggta ctgcaagata cttggctgtt tacaggaagt atctatgaca
    21781 atatcaaata tgggaacgac gatgcaacag acgaacaaat cattgaggct gctaaagctg
    21841 ctcacgtaga cgattttgtt cgtaaattgc cagaaggata taacactgta ttaaacgagg
    21901 atgcaagcaa tatttcgcaa gggcagcgtc agctaatcac aattgcgcgg gcattcttag
    21961 caaatccaga tgtcctgatc cttgatgaag caacatctag tgtagataca cgaacagaaa
    22021 tactgattca aaaagcgatg aaccgcctat tggaaaaccg gacaagtttc gttgttgctc
    22081 accgcttgtc tacaatacgc gatgcagaca acattattgt gatgaatcat ggttcgatcg
    22141 tagaaacagg aaaccacgat acattgatgg aaaaagacgg attctatgca gatctttata
    22201 acagtcagtt ttctgaagaa gaagcttcat agaatagtac aaatttatta ttttttttag
    22261 gggctgtgac aaaagtcgtg tcacagtccc tttttccgaa taaacggtgt tcaatcagct
    22321 gtccttcggt attagctcaa aaaacggcaa aatatgagaa gctatttttc cgttttgtat
    22381 ctctaatact caggtcaaac cgctgatttt acaacctctt ccttaaacgg tgttctcctg
    22441 ccaactcctc ggtcacaagt cacaatagtc aacaaacctg agaagctgtt ttttgaaatt
    22501 gctccttgtg actcggagtt aaacggcagg ctcacaacct cttggtaaac ggtgttcaaa
    22561 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
    22621 atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
    22681 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg
    22741 tttggctgtt cttattactt gatgcagaac agctttttca caacctcttt ttataagagt
    22801 ttttactcat taatttacaa aaaacaagca aaatgcgaat tttctcacat tttttttaac
    22861 aaaatcaaag ttattgcttg aaagaagaag aatccatcgt taaaataaag cttgacgtag
    22921 aggggagcaa gaaatatgaa agattttcaa ttgggggctg ccacctattt aaaaagtgta
    22981 gccattcggg tgaaagaccg agataaaatg attgattttt acaagaacgt tgttggcttt
    23041 gatttgaaaa gagaagaaaa cgaattagcc attttaggaa cagaagaagc tggaagtgaa
    23101 atgttgctgc tggaagaaag tcctagagcg aatgatcaca tgggggtgat caaaaagctg
    23161 aaccgttttt cattgattat ccctagcaca gaagaaatgg cagatatcct ttgtcgtatc
    23221 cgtaaaatga attatccaat caaagaagct ttagaagatc atggacgtat ggggattttg
    23281 ctcgtagacc cagaagagaa tgaattggaa atttaccatg aagagaataa gaacgaccaa
    23341 agagaaattc cagaaacaat ggatcaagaa gcattattaa gaaaatctga agaaaaattc
    23401 agtaaactat caacaggttc ttattttgaa aaaatccatt taaatgttac taacttaaca
    23461 aaagaacgcc aatttcttaa tcatgtactt ggtttgaagg tacataatga agaagagggc
    23521 atccatgtat tgaatgaagg agatttccat gttggtttga cagaagctag cggaggcgca
    23581 attgatttgc ctacagatga agtgattgga cttgattttc tgaaattcat gatcgatact
    23641 gaaagcatgg aaggactgat tgctcacttg cagcaactat cagttgattt cttcttagac
    23701 aaaaagcaca cagtactcac aatctatgat gctataggaa tcgaatggtg gttcgtcagt
    23761 gagccaaaag aaaagaataa aactgacatt cgatgcgacg cttaactttt tctttcttaa
    23821 cgattgtcgc atgtctcgtc attttatgaa cggtcatact atcgcttatt ctagagatag
    23881 aaagagaaaa tagtataccg ggggaaactg cgcggttttt cgcgcagttt ttttgtgtaa
    23941 aaaaggttga ttcctcatag attaagcttt aagcttggac tgtaagaatc tgatagaaaa
    24001 gcaggagaaa aagatgtatc cacacacgaa acagcttatt ttgaaaaaga tggaagaagg
    24061 attttatcca ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa catttacagc
    24121 cggctttgcc caagtcgttc ctgaaaagca gctgatgaca gaagatctgt tgtttgatgc
    24181 agcctcactg acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat tagaagatgg
    24241 aaagatagaa ctcgatcaac cgcttcataa atatcttcct gaatttgaaa atcagacaat
    24301 cactttgcgt catttactga cacatacttc tgatatcaaa acatggatac caaacagaga
    24361 taagctatct caggaagaat tgatcgccgc ttatttgaag ctagaaccag gtgagcaact
    24421 aggaaaagaa gtaaaatata cagatgccgg tacgattctt cttgggttga tgctagagga
    24481 aatttaccaa gaaactgcag tggaaatctt tcaagaacaa gtattggaac cattaggaat
    24541 gatgaacagc ttatttcttc cttttccttc caaaaaaatc gttcccacag aacaactgcc
    24601 ttcgggtgag gttttgcgtg gacaaactca tgatccgaaa gcaagagttt tggctgaaca
    24661 tgcaggtaac gcgggtctct ttattgattt gaaagacagc attacttttg tccaaatgta
    24721 tttgaacaag ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga ttgattcttt
    24781 actgcaagat caaacaccga aaaaaaatgg ctatcgttct ttaggatggg acttaaaaaa
    24841 ggatgtagaa gacggtcatg cgattctttt tcatactggt tatacaggta cgtttttctt
    24901 gattgatata aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga
    24961 tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa
    25021 agcggtatta ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg
    25081 caggaaccta tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta
    25141 cgttcagtct ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt
    25201 gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat
    25261 gaactatggt cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta
    25321 ttgatcaaga tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc
    25381 tatgggatga aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct
    25441 gaacctggtg cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag
    25501 atgatcaaag atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat
    25561 ttcttttatg taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa
    25621 acacagcaaa gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa
    25681 ggacaaacaa gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa
    25741 acaccagaat tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta
    25801 gaaactgagt ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa
    25861 caagcccctt atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga
    25921 aaaacatatc cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg
    25981 gcggtccaag gtgaactagc aatcattgcg tctgaaccag gcaaatagtt cccagatagc
    26041 tgaaagcagc agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata
    26101 aatagaagtc tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg
    26161 tctatgaaac aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta
    26221 agtctgttta tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg
    26281 cacaccacat tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc
    26341 agttattcag gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct
    26401 tctttagggt tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg
    26461 ccgcttgccg gcatcgtata tgaacaattt gtttctagat tccatgtgaa attcaaggct
    26521 ggatatgaaa tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata
    26581 attggaatcg tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt
    26641 gtgtcttggc tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta
    26701 aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa
    26761 ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt
    26821 ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt
    26881 ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg
    26941 ctgcgaccgt atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt
    27001 caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt
    27061 gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt
    27121 gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa
    27181 acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct
    27241 tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca
    27301 gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta
    27361 aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa
    27421 agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa
    27481 gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa
    27541 aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact
    27601 atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca
    27661 aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa
    27721 ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg
    27781 gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc
    27841 cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa
    27901 aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga
    27961 agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat
    28021 tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga
    28081 cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc
    28141 gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga
    28201 tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg
    28261 atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt
    28321 agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt
    28381 ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt
    28441 gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac
    28501 attgacgatg gagcgtgtga cgaaaaatca tttactgaca aatgagacat ataatagtct
    28561 ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac
    28621 aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac
    28681 gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt
    28741 aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac
    28801 tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga
    28861 agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa
    28921 tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat
    28981 cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat
    29041 cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt
    29101 gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa
    29161 aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg
    29221 tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga
    29281 agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga
    29341 gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt
    29401 actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg
    29461 cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg
    29521 gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt
    29581 ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc
    29641 gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct
    29701 ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat
    29761 catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca
    29821 gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc
    29881 taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg
    29941 agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga
    30001 tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga
    30061 ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg
    30121 tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa
    30181 aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc
    30241 aagtaatggc gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt tttgaacatg
    30301 gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa
    30361 ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag
    30421 cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt
    30481 ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc
    30541 catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa
    30601 agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa
    30661 atcaacataa gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa
    30721 caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg
    30781 ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag
    30841 gaacagaaag ctctggaaaa gatactcata catcggcagg attcgtatct cctgaccaat
    30901 gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga
    30961 gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg
    31021 gatgctatgt ttctttacca tactatccag taatagattg actagagaga ttacctgctt
    31081 tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa
    31141 actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaagaga gatgcgctgg
    31201 caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac
    31261 acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag
    31321 tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt
    31381 tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt
    31441 ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt
    31501 cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag
    31561 cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc
    31621 aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc taaaatctct
    31681 tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa
    31741 atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc
    31801 cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt
    31861 tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt
    31921 ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt
    31981 tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa
    32041 tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg
    32101 cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt
    32161 ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc
    32221 aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta
    32281 ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat
    32341 ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc
    32401 atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa
    32461 tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
    32521 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa
    32581 aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
    32641 cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt
    32701 aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag
    32761 cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc
    32821 tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga
    32881 tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa
    32941 atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt
    33001 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
    33061 tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
    33121 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
    33181 caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat
    33241 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
    33301 gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg
    33361 cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt
    33421 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
    33481 gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct
    33541 tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat
    33601 tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa
    33661 ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg
    33721 cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc
    33781 gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat
    33841 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
    33901 agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc
    33961 tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat
    34021 tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct
    34081 acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat
    34141 attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga
    34201 tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc
    34261 acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat
    34321 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
    34381 gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt
    34441 cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga
    34501 gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa
    34561 gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat
    34621 tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga
    34681 gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
    34741 gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
    34801 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
    34861 ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc
    34921 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
    34981 aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
    35041 caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt
    35101 attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct
    35161 ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg
    35221 tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca
    35281 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct
    35341 ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg
    35401 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
    35461 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
    35521 atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg
    35581 ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt
    35641 catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta
    35701 cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac
    35761 ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa
    35821 gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt
    35881 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
    35941 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt
    36001 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
    36061 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
    36121 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
    36181 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
    36241 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
    36301 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
    36361 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
    36421 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
    36481 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
    36541 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
    36601 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
    36661 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
    36721 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
    36781 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
    36841 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
    36901 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
    36961 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
    37021 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
    37081 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
    37141 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
    37201 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
    37261 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
    37321 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
    37381 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
    37441 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
    37501 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
    37561 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
    37621 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
    37681 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
    37741 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
    37801 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
    37861 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
    37921 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
    37981 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
    38041 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
    38101 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
    38161 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
    38221 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
    38281 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
    38341 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
    38401 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
    38461 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
    38521 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
    38581 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
    38641 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
    38701 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
    38761 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
    38821 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
    38881 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
    38941 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
    39001 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
    39061 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
    39121 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
    39181 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
    39241 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
    39301 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
    39361 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
    39421 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
    39481 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
    39541 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
    39601 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
    39661 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
    39721 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
    39781 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
    39841 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
    39901 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
    39961 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
    40021 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
    40081 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
    40141 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
    40201 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
    40261 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
    40321 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
    40381 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
    40441 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
    40501 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
    40561 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
    40621 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
    40681 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
    40741 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
    40801 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
    40861 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
    40921 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
    40981 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
    41041 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
    41101 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
    41161 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
    41221 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
    41281 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
    41341 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
    41401 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
    41461 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
    41521 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
    41581 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
    41641 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
    41701 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
    41761 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
    41821 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
    41881 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
    41941 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
    42001 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
    42061 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
    42121 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
    42181 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
    42241 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
    42301 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
    42361 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
    42421 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
    42481 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
    42541 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
    42601 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
    42661 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
    42721 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
    42781 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
    42841 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
    42901 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
    42961 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
    43021 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
    43081 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
    43141 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
    43201 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
    43261 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
    43321 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
    43381 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
    43441 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
    43501 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
    43561 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
    43621 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
    43681 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
    43741 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
    43801 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
    43861 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
    43921 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
    43981 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
    44041 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
    44101 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
    44161 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
    44221 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
    44281 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
    44341 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
    44401 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
    44461 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
    44521 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
    44581 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
    44641 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
    44701 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
    44761 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
    44821 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
    44881 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
    44941 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
    45001 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
    45061 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
    45121 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
    45181 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
    45241 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
    45301 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
    45361 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
    45421 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
    45481 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
    45541 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
    45601 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
    45661 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
    45721 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
    45781 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
    45841 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
    45901 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
    45961 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    46021 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    46081 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    46141 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    46201 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    46261 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    46321 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    46381 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    46441 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    46501 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    46561 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    46621 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    46681 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    46741 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    46801 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    46861 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    46921 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    46981 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    47041 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    47101 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    47161 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    47221 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    47281 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    47341 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    47401 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    47461 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    47521 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    47581 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    47641 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    47701 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    47761 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    47821 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    47881 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    47941 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    48001 tgtaggaaaa taaaattgta ggaaaataaa aaggagctgt tgtttgtggt agattacaaa
    48061 acgtttgacc ctgatctttg ggctgcgatc gccaaagaag aagaaagaca agaacataat
    48121 ttggagctga ttgcctctga aaactttgtc tccgaagcag tgatggctgc acaaggaagt
    48181 atcttgacaa ataaatacgc agaaggttat ccagggcatc gctattatgg tgggtgtgaa
    48241 tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa
    48301 tttgcgaacg tccagcctca ctccggctct caagccaaca cggctgctta tctcgctttg
    48361 gtagaaccag gggacacgat cttaggaatg gatctttccg caggcggaca tctaacacat
    48421 ggatcaccag tcaacttcag tggcaaaacc tatcactttg ttgcttacgg tgttgatccg
    48481 acaacagaag ttattgatta taatgtggta cgtattttag ctagaaaaca tcaaccaaaa
    48541 ttgatcgtag caggtgccag tgcttacgga cgaacgatcg actttgcgaa attcagagaa
    48601 atcgcagatg aagtaggcgc aaaactgatg gtagatatgg cacatatcgc aggattagta
    48661 gcagcaggac ttcatcctaa tccagttcct tatgcagata tcacaacgac taccactcat
    48721 aaaacattgc gtggccctcg aggcggcatg attttgacaa acgatgaagc attagcaaaa
    48781 aaaataaata gtgcggtatt tcctggtatt caaggcggcc ctctagagca tgtgattgcg
    48841 ggtaaagcag tagcattcaa agaagcatta gatcctgcat ttaaagaata tagtgaacaa
    48901 atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg aaccagagtc
    48961 atttcagggg cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat
    49021 ggaaaagaag cagagagcat tttggacagt gtgaatatca ctgtaaataa aaactcgatt
    49081 ccttttgaaa cactaagtcc attcaaaaca agcggtattc gaatcggcac gccagcaatc
    49141 actactcgcg ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca
    49201 ttacaagcaa aagatgataa cgctcaacta gacgaagtca aaacaggtgt acgcgaatta
    49261 actgaaaaat ttccgttgca taaaaaataa aaatttaaat tccattagta aacagaaaag
    49321 ttcaatgttg ggcttttctg tttatttttt gggaaaacgg attctttgac aatatcggtg
    49381 atgctctcta gtatatgagg tgtaatttta gtcttcacac taaatctcag ggaggaaaag
    49441 tcatgactga tcgtatctcg ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc
    49501 ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag agttttacta gaagaagtac
    49561 aagaatcacc tgtgaacaaa tattccgtcg atattgaaga cttgatggtg gatactggcg
    49621 aatggggaaa actcaatatt cgtctcgtac gaccagaagg gaatatggat aaattaccag
    49681 ttatttttta tattcatgga gcaggctggg tttttggcag tgctcaaacc catgataaat
    49741 tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat
    49801 caccagaagc aaaatatcca acagcaattg aacaaaatta tgctgtgttg caacagctga
    49861 ataaattagc caagtcaaaa aatctggatc tcgagaatct aatcgttgca ggagattcag
    49921 taggtggtaa catggctaca gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa
    49981 tatccaagca attgctgtat tatccagtca caaacgctga ttttgataca gattcctatc
    50041 atgaattcgg agaaaactat tttttgacaa gagaaggaat gatgtggttt tgggaccaat
    50101 atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa
    50161 atgaactaaa tgatcttcct tcagcaatga ttttaacggg agaagcggat gtattaagag
    50221 atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac
    50281 gcttccaagg aatcatccat gattttgtca tggtaaatgc tatggatcag acaaaggcaa
    50341 cgcgtgcagc aatggcatta tctacttctt ggatcaagaa aaaagaataa gctttgcttg
    50401 tccgcaggaa aacttctttt caaaaacgga ttttcttagg aaaaatggcg agtttgacta
    50461 gtcaatcact tgattttgtt ttacaattaa aaagaaacga cagaacaaag gagagcttaa
    50521 aaaatgggca aatttcaagt gatcgatcat ccattgattc aacacaaact aactattatt
    50581 cgggacaaaa actgcggaac aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg
    50641 atggcatatg aagtatcacg ggatatgcca ttggaagaca tcgtgatcga aacaccgatc
    50701 actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag cgattattcc aatcttgcgc
    50761 gcaggtattg gaatggttga cggtatcttg gaattgattc cagctgctaa agtgggacat
    50821 atcggtcttt atcgagatga agaaacattg gaaccacatg aatatttcgt caaacttcca
    50881 gaagacattg atgcacgtca gttgtttgta gtagatccaa tgttggcaac aggcggatca
    50941 gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt
    51001 ttggtagcag cacctgaggg agtaaaagct ttgcaagaag cgcatcctga tattgacatc
    51061 tatacagctt ctctagatga aaaattgaat gaacatggtt atatcgttcc tggactaggg
    51121 gatgcgggtg accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa
    51181 cttttgtcac atcctttttt ggtcattttt aacgtttgat gcagaacagc tttttcacag
    51241 ctttcgtttg catataaaaa aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt
    51301 ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca agttcgcatt
    51361 ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg aaatcgttcc
    51421 agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga atttttcacc
    51481 aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc
    51541 tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta aactaatttt
    51601 ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga taaggccagc
    51661 ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc gatccctata
    51721 gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga tacatctgtc
    51781 tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat tatttctata
    51841 aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat cacatgaact
    51901 gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa caatcttgcc
    51961 accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt atcataagta
    52021 ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg aatgctgaac
    52081 ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag tatcatgata
    52141 ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc tgttaaaccc
    52201 catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac attgataaaa
    52261 aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt tttcgtatct
    52321 tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga aaaaatgcta
    52381 ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat agtgcccaat
    52441 atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat aactcctact
    52501 attcagtttt tgaagataga gtcagtatac atgaagagtt a
//
LOCUS       DADVXO010000005        50950 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.005, whole genome shotgun sequence.
ACCESSION   DADVXO010000005 DADVXO010000000
VERSION     DADVXO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.005"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            232..603
                     /locus_tag="KB950_000243"
     CDS             232..603
                     /locus_tag="KB950_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBB6856487.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(877..1569)
                     /locus_tag="KB950_000244"
     CDS             complement(877..1569)
                     /locus_tag="KB950_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6856488.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1572..2357)
                     /locus_tag="KB950_000245"
     CDS             complement(1572..2357)
                     /locus_tag="KB950_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6856489.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2594..3994
                     /gene="lacG"
                     /locus_tag="KB950_000246"
     CDS             2594..3994
                     /gene="lacG"
                     /locus_tag="KB950_000246"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBB6856490.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4019..4336
                     /locus_tag="KB950_000247"
     CDS             4019..4336
                     /locus_tag="KB950_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6856491.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4349..6022
                     /locus_tag="KB950_000248"
     CDS             4349..6022
                     /locus_tag="KB950_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBB6856492.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6036..7667
                     /locus_tag="KB950_000249"
     CDS             6036..7667
                     /locus_tag="KB950_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBB6856493.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8158..9723)
                     /gene="guaA"
                     /locus_tag="KB950_000250"
     CDS             complement(8158..9723)
                     /gene="guaA"
                     /locus_tag="KB950_000250"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBB6856494.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9782..10705)
                     /locus_tag="KB950_000251"
     CDS             complement(9782..10705)
                     /locus_tag="KB950_000251"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBB6856495.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10901..11506
                     /locus_tag="KB950_000252"
     CDS             10901..11506
                     /locus_tag="KB950_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6856496.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11814..12830
                     /locus_tag="KB950_000253"
     CDS             11814..12830
                     /locus_tag="KB950_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6856497.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12971..14272
                     /locus_tag="KB950_000254"
     CDS             12971..14272
                     /locus_tag="KB950_000254"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBB6856498.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14318..14980
                     /gene="deoC"
                     /locus_tag="KB950_000255"
     CDS             14318..14980
                     /gene="deoC"
                     /locus_tag="KB950_000255"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBB6856499.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15001..15399
                     /locus_tag="KB950_000256"
     CDS             15001..15399
                     /locus_tag="KB950_000256"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBB6856500.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15465..16547
                     /locus_tag="KB950_000257"
     CDS             15465..16547
                     /locus_tag="KB950_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6856501.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16759..18321
                     /locus_tag="KB950_000258"
     CDS             16759..18321
                     /locus_tag="KB950_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6856502.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18314..19447
                     /locus_tag="KB950_000259"
     CDS             18314..19447
                     /locus_tag="KB950_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856503.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19447..20403
                     /locus_tag="KB950_000260"
     CDS             19447..20403
                     /locus_tag="KB950_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856504.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20502..21668
                     /gene="deoB"
                     /locus_tag="KB950_000261"
     CDS             20502..21668
                     /gene="deoB"
                     /locus_tag="KB950_000261"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBB6856505.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21692..22510
                     /locus_tag="KB950_000262"
     CDS             21692..22510
                     /locus_tag="KB950_000262"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBB6856506.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22527..23234
                     /gene="deoD"
                     /locus_tag="KB950_000263"
     CDS             22527..23234
                     /gene="deoD"
                     /locus_tag="KB950_000263"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBB6856507.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23532..24218)
                     /gene="gpmA"
                     /locus_tag="KB950_000264"
     CDS             complement(23532..24218)
                     /gene="gpmA"
                     /locus_tag="KB950_000264"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBB6856508.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24390..24971)
                     /locus_tag="KB950_000265"
     CDS             complement(24390..24971)
                     /locus_tag="KB950_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6856509.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25104..25787)
                     /gene="rpiA"
                     /locus_tag="KB950_000266"
     CDS             complement(25104..25787)
                     /gene="rpiA"
                     /locus_tag="KB950_000266"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBB6856510.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26106..26519
                     /gene="rpsL"
                     /locus_tag="KB950_000267"
     CDS             26106..26519
                     /gene="rpsL"
                     /locus_tag="KB950_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBB6856511.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26603..27073
                     /gene="rpsG"
                     /locus_tag="KB950_000268"
     CDS             26603..27073
                     /gene="rpsG"
                     /locus_tag="KB950_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBB6856512.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27167..29254
                     /gene="fusA"
                     /locus_tag="KB950_000269"
     CDS             27167..29254
                     /gene="fusA"
                     /locus_tag="KB950_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBB6856513.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29426..30613
                     /gene="tuf"
                     /locus_tag="KB950_000270"
     CDS             29426..30613
                     /gene="tuf"
                     /locus_tag="KB950_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBB6856514.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31020..31328
                     /gene="rpsJ"
                     /locus_tag="KB950_000271"
     CDS             31020..31328
                     /gene="rpsJ"
                     /locus_tag="KB950_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBB6856515.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31359..31988
                     /gene="rplC"
                     /locus_tag="KB950_000272"
     CDS             31359..31988
                     /gene="rplC"
                     /locus_tag="KB950_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBB6856516.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32016..32639
                     /gene="rplD"
                     /locus_tag="KB950_000273"
     CDS             32016..32639
                     /gene="rplD"
                     /locus_tag="KB950_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBB6856517.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32639..32929
                     /gene="rplW"
                     /locus_tag="KB950_000274"
     CDS             32639..32929
                     /gene="rplW"
                     /locus_tag="KB950_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBB6856518.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32968..33801
                     /gene="rplB"
                     /locus_tag="KB950_000275"
     CDS             32968..33801
                     /gene="rplB"
                     /locus_tag="KB950_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBB6856519.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33844..34122
                     /gene="rpsS"
                     /locus_tag="KB950_000276"
     CDS             33844..34122
                     /gene="rpsS"
                     /locus_tag="KB950_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBB6856520.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34145..34492
                     /gene="rplV"
                     /locus_tag="KB950_000277"
     CDS             34145..34492
                     /gene="rplV"
                     /locus_tag="KB950_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBB6856521.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34506..35162
                     /gene="rpsC"
                     /locus_tag="KB950_000278"
     CDS             34506..35162
                     /gene="rpsC"
                     /locus_tag="KB950_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBB6856522.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35165..35599
                     /gene="rplP"
                     /locus_tag="KB950_000279"
     CDS             35165..35599
                     /gene="rplP"
                     /locus_tag="KB950_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBB6856523.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35589..35777
                     /gene="rpmC"
                     /locus_tag="KB950_000280"
     CDS             35589..35777
                     /gene="rpmC"
                     /locus_tag="KB950_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBB6856524.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35802..36068
                     /gene="rpsQ"
                     /locus_tag="KB950_000281"
     CDS             35802..36068
                     /gene="rpsQ"
                     /locus_tag="KB950_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBB6856525.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36126..36494
                     /gene="rplN"
                     /locus_tag="KB950_000282"
     CDS             36126..36494
                     /gene="rplN"
                     /locus_tag="KB950_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBB6856526.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36531..36839
                     /gene="rplX"
                     /locus_tag="KB950_000283"
     CDS             36531..36839
                     /gene="rplX"
                     /locus_tag="KB950_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBB6856527.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36867..37406
                     /gene="rplE"
                     /locus_tag="KB950_000284"
     CDS             36867..37406
                     /gene="rplE"
                     /locus_tag="KB950_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBB6856528.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37425..37610
                     /locus_tag="KB950_000285"
     CDS             37425..37610
                     /locus_tag="KB950_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBB6856529.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37646..38044
                     /gene="rpsH"
                     /locus_tag="KB950_000286"
     CDS             37646..38044
                     /gene="rpsH"
                     /locus_tag="KB950_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBB6856530.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38076..38612
                     /gene="rplF"
                     /locus_tag="KB950_000287"
     CDS             38076..38612
                     /gene="rplF"
                     /locus_tag="KB950_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBB6856531.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38773..39129
                     /gene="rplR"
                     /locus_tag="KB950_000288"
     CDS             38773..39129
                     /gene="rplR"
                     /locus_tag="KB950_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBB6856532.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39150..39650
                     /gene="rpsE"
                     /locus_tag="KB950_000289"
     CDS             39150..39650
                     /gene="rpsE"
                     /locus_tag="KB950_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBB6856533.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39665..39844
                     /gene="rpmD"
                     /locus_tag="KB950_000290"
     CDS             39665..39844
                     /gene="rpmD"
                     /locus_tag="KB950_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBB6856534.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39888..40328
                     /gene="rplO"
                     /locus_tag="KB950_000291"
     CDS             39888..40328
                     /gene="rplO"
                     /locus_tag="KB950_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBB6856535.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40328..41623
                     /gene="secY"
                     /locus_tag="KB950_000292"
     CDS             40328..41623
                     /gene="secY"
                     /locus_tag="KB950_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBB6856536.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41685..42332
                     /locus_tag="KB950_000293"
     CDS             41685..42332
                     /locus_tag="KB950_000293"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBB6856537.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42523..42741
                     /gene="infA"
                     /locus_tag="KB950_000294"
     CDS             42523..42741
                     /gene="infA"
                     /locus_tag="KB950_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBB6856538.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42774..42890
                     /gene="rpmJ"
                     /locus_tag="KB950_000295"
     CDS             42774..42890
                     /gene="rpmJ"
                     /locus_tag="KB950_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBB6856539.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42908..43273
                     /gene="rpsM"
                     /locus_tag="KB950_000296"
     CDS             42908..43273
                     /gene="rpsM"
                     /locus_tag="KB950_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBB6856540.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43301..43690
                     /gene="rpsK"
                     /locus_tag="KB950_000297"
     CDS             43301..43690
                     /gene="rpsK"
                     /locus_tag="KB950_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBB6856541.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43770..44708
                     /locus_tag="KB950_000298"
     CDS             43770..44708
                     /locus_tag="KB950_000298"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBB6856542.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44736..45116
                     /gene="rplQ"
                     /locus_tag="KB950_000299"
     CDS             44736..45116
                     /gene="rplQ"
                     /locus_tag="KB950_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBB6856543.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45388..46227
                     /locus_tag="KB950_000300"
     CDS             45388..46227
                     /locus_tag="KB950_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6856544.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46203..47072
                     /locus_tag="KB950_000301"
     CDS             46203..47072
                     /locus_tag="KB950_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6856545.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47062..47859
                     /locus_tag="KB950_000302"
     CDS             47062..47859
                     /locus_tag="KB950_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB6856546.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48062..48808
                     /gene="truA"
                     /locus_tag="KB950_000303"
     CDS             48062..48808
                     /gene="truA"
                     /locus_tag="KB950_000303"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBB6856547.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49473..49710
                     /locus_tag="KB950_000304"
                     /pseudo
     CDS             49473..49710
                     /locus_tag="KB950_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49854..50630)
                     /locus_tag="KB950_000305"
     CDS             complement(49854..50630)
                     /locus_tag="KB950_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6856548.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50753..>50950
                     /locus_tag="KB950_000306"
     CDS             50753..>50950
                     /locus_tag="KB950_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6856549.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHA"
BASE COUNT        16156 a         9270 c        11061 g        14463 t
ORIGIN      
        1 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
       61 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
      121 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
      181 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
      241 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
      301 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
      361 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
      421 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
      481 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
      541 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
      601 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
      661 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
      721 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
      781 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
      841 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
      901 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
      961 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
     1021 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
     1081 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
     1141 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
     1201 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
     1261 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
     1321 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
     1381 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
     1441 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
     1501 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
     1561 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
     1621 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
     1681 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
     1741 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
     1801 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
     1861 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
     1921 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
     1981 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
     2041 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
     2101 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
     2161 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
     2221 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
     2281 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
     2341 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
     2401 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
     2461 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
     2521 tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
     2581 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
     2641 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
     2701 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
     2761 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
     2821 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
     2881 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
     2941 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
     3001 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
     3061 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
     3121 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
     3181 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
     3241 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
     3301 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
     3361 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
     3421 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
     3481 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
     3541 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
     3601 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
     3661 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
     3721 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
     3781 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
     3841 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
     3901 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
     3961 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
     4021 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
     4081 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
     4141 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
     4201 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
     4261 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
     4321 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
     4381 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
     4441 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
     4501 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
     4561 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
     4621 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
     4681 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
     4741 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
     4801 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
     4861 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
     4921 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
     4981 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
     5041 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
     5101 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
     5161 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
     5221 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
     5281 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
     5341 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
     5401 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
     5461 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
     5521 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
     5581 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
     5641 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
     5701 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgggatgtt
     5761 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
     5821 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
     5881 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
     5941 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
     6001 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
     6061 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
     6121 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
     6181 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
     6241 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
     6301 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
     6361 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
     6421 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
     6481 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
     6541 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
     6601 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
     6661 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
     6721 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
     6781 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
     6841 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
     6901 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
     6961 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
     7021 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
     7081 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
     7141 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
     7201 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
     7261 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
     7321 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
     7381 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
     7441 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
     7501 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
     7561 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
     7621 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
     7681 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
     7741 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
     7801 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
     7861 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
     7921 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
     7981 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
     8041 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
     8101 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
     8161 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
     8221 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
     8281 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
     8341 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
     8401 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
     8461 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
     8521 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
     8581 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
     8641 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
     8701 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
     8761 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
     8821 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
     8881 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
     8941 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
     9001 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
     9061 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
     9121 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
     9181 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
     9241 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
     9301 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
     9361 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
     9421 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
     9481 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
     9541 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
     9601 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
     9661 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
     9721 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
     9781 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
     9841 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
     9901 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
     9961 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    10021 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    10081 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    10141 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    10201 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    10261 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    10321 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    10381 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    10441 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    10501 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    10561 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    10621 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    10681 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    10741 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    10801 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    10861 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    10921 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    10981 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    11041 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    11101 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    11161 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    11221 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    11281 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    11341 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    11401 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    11461 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    11521 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    11581 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    11641 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
    11701 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    11761 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    11821 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    11881 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    11941 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    12001 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    12061 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    12121 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    12181 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    12241 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    12301 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    12361 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    12421 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    12481 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    12541 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    12601 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    12661 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    12721 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    12781 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    12841 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    12901 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    12961 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    13021 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    13081 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    13141 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    13201 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    13261 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    13321 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    13381 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    13441 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    13501 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    13561 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    13621 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    13681 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    13741 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    13801 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    13861 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    13921 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    13981 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    14041 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    14101 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    14161 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    14221 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    14281 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    14341 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    14401 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    14461 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    14521 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    14581 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    14641 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    14701 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    14761 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    14821 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    14881 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    14941 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    15001 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    15061 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    15121 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    15181 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    15241 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    15301 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    15361 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    15421 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    15481 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    15541 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    15601 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    15661 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    15721 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    15781 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    15841 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    15901 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    15961 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    16021 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    16081 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    16141 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    16201 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    16261 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    16321 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    16381 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    16441 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    16501 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    16561 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    16621 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    16681 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    16741 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    16801 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    16861 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    16921 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    16981 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    17041 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    17101 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    17161 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    17221 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    17281 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    17341 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    17401 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    17461 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    17521 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    17581 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    17641 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    17701 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    17761 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    17821 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    17881 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    17941 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    18001 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    18061 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    18121 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    18181 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    18241 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    18301 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    18361 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    18421 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    18481 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    18541 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    18601 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    18661 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    18721 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    18781 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    18841 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    18901 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    18961 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    19021 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    19081 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    19141 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    19201 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    19261 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    19321 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    19381 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    19441 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    19501 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    19561 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    19621 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    19681 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    19741 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    19801 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    19861 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    19921 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    19981 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    20041 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    20101 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    20161 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    20221 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    20281 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    20341 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    20401 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    20461 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    20521 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    20581 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    20641 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    20701 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    20761 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    20821 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    20881 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    20941 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    21001 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    21061 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    21121 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    21181 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    21241 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    21301 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    21361 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    21421 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    21481 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    21541 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    21601 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    21661 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    21721 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    21781 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    21841 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    21901 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    21961 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    22021 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    22081 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    22141 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    22201 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    22261 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    22321 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    22381 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    22441 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    22501 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    22561 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    22621 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    22681 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    22741 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    22801 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    22861 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    22921 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    22981 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    23041 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    23101 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    23161 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    23221 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    23281 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    23341 ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca
    23401 atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc
    23461 aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt
    23521 tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa
    23581 acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg
    23641 atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg
    23701 tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact
    23761 ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt
    23821 gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc
    23881 cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt
    23941 aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga
    24001 tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa
    24061 gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg
    24121 attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat
    24181 tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata
    24241 ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc
    24301 catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca
    24361 gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca
    24421 ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga agaaataaat
    24481 tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg
    24541 atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta
    24601 cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt
    24661 tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct
    24721 ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg
    24781 caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca
    24841 gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca
    24901 gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca
    24961 ataattttca tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa
    25021 tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta
    25081 ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct
    25141 ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc
    25201 ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc
    25261 agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc
    25321 aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg
    25381 atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg
    25441 atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg
    25501 ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg
    25561 actacgggga cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt
    25621 gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca
    25681 atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc
    25741 tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc
    25801 tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga
    25861 taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat
    25921 gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac
    25981 gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat
    26041 cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac
    26101 aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat
    26161 ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa
    26221 actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga
    26281 actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt
    26341 atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc
    26401 gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg
    26461 gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat
    26521 tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat
    26581 atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt
    26641 accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga
    26701 cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc
    26761 tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct
    26821 tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc
    26881 tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga
    26941 acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc
    27001 ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca
    27061 ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt
    27121 ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt
    27181 ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac
    27241 tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga
    27301 aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc
    27361 tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca
    27421 cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt
    27481 tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac
    27541 ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt
    27601 atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc
    27661 aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat
    27721 gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga
    27781 attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact
    27841 tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg
    27901 ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa
    27961 aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc
    28021 agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag
    28081 cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac
    28141 tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa
    28201 aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat
    28261 ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg
    28321 ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc
    28381 agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc
    28441 tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg
    28501 cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg
    28561 tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt
    28621 ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata
    28681 cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa
    28741 tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa
    28801 agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact
    28861 ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc
    28921 tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc
    28981 tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg
    29041 tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat
    29101 ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg
    29161 tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga
    29221 aatcattaag aaaaacggcg gaaaagctga ataatttctt gaaaccggat agtcctgcaa
    29281 agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga
    29341 tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat
    29401 tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca
    29461 cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat
    29521 cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg
    29581 tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac
    29641 agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat
    29701 gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc
    29761 aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt
    29821 tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat
    29881 ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc
    29941 tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt
    30001 aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat
    30061 gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt
    30121 tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac
    30181 ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc
    30241 tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca
    30301 agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt
    30361 gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt
    30421 ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt
    30481 tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa
    30541 cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga
    30601 aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc
    30661 tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa
    30721 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
    30781 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
    30841 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
    30901 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
    30961 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
    31021 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
    31081 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
    31141 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
    31201 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
    31261 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
    31321 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
    31381 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
    31441 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
    31501 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
    31561 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
    31621 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
    31681 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
    31741 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
    31801 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
    31861 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
    31921 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
    31981 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
    32041 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
    32101 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
    32161 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
    32221 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
    32281 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
    32341 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
    32401 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
    32461 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
    32521 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
    32581 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
    32641 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
    32701 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
    32761 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
    32821 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
    32881 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
    32941 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
    33001 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
    33061 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
    33121 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
    33181 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
    33241 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
    33301 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
    33361 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
    33421 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
    33481 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
    33541 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
    33601 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
    33661 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
    33721 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
    33781 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
    33841 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
    33901 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
    33961 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
    34021 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
    34081 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
    34141 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
    34201 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
    34261 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
    34321 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
    34381 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
    34441 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
    34501 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
    34561 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
    34621 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
    34681 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
    34741 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
    34801 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
    34861 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
    34921 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
    34981 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
    35041 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
    35101 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
    35161 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
    35221 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
    35281 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
    35341 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
    35401 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
    35461 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
    35521 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
    35581 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
    35641 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
    35701 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
    35761 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
    35821 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
    35881 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
    35941 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
    36001 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
    36061 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
    36121 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
    36181 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
    36241 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
    36301 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
    36361 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
    36421 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
    36481 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
    36541 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
    36601 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
    36661 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
    36721 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
    36781 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
    36841 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
    36901 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
    36961 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
    37021 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
    37081 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
    37141 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
    37201 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
    37261 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
    37321 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
    37381 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
    37441 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
    37501 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
    37561 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
    37621 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
    37681 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
    37741 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
    37801 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
    37861 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
    37921 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
    37981 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
    38041 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
    38101 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
    38161 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
    38221 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
    38281 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
    38341 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
    38401 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
    38461 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
    38521 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
    38581 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
    38641 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
    38701 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
    38761 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
    38821 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
    38881 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
    38941 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
    39001 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
    39061 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
    39121 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
    39181 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
    39241 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
    39301 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
    39361 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
    39421 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
    39481 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
    39541 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
    39601 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
    39661 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
    39721 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
    39781 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
    39841 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
    39901 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
    39961 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
    40021 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
    40081 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
    40141 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
    40201 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
    40261 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
    40321 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
    40381 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
    40441 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
    40501 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
    40561 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
    40621 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
    40681 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    40741 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    40801 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    40861 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    40921 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    40981 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    41041 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    41101 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    41161 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    41221 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    41281 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    41341 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    41401 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    41461 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    41521 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    41581 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    41641 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    41701 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    41761 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    41821 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    41881 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    41941 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    42001 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    42061 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    42121 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    42181 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    42241 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    42301 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    42361 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    42421 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    42481 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    42541 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    42601 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    42661 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    42721 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    42781 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    42841 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    42901 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    42961 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    43021 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    43081 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    43141 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    43201 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    43261 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    43321 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    43381 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    43441 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    43501 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    43561 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    43621 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    43681 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    43741 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    43801 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    43861 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    43921 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    43981 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    44041 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    44101 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    44161 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    44221 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    44281 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    44341 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    44401 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    44461 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    44521 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    44581 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    44641 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    44701 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    44761 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    44821 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    44881 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    44941 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    45001 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    45061 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    45121 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    45181 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    45241 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    45301 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    45361 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    45421 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    45481 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    45541 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    45601 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    45661 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    45721 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    45781 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    45841 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    45901 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    45961 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    46021 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    46081 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    46141 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    46201 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    46261 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    46321 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    46381 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    46441 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    46501 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    46561 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    46621 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    46681 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    46741 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    46801 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    46861 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    46921 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    46981 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    47041 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    47101 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    47161 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    47221 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    47281 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    47341 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    47401 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    47461 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    47521 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    47581 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    47641 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    47701 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    47761 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    47821 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    47881 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    47941 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    48001 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    48061 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    48121 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    48181 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    48241 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    48301 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    48361 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    48421 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    48481 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    48541 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    48601 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    48661 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    48721 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    48781 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    48841 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    48901 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    48961 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    49021 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    49081 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    49141 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    49201 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    49261 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    49321 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    49381 tttatattta aatggcacaa atttttctaa ttatttgtca tttagcgctg aaaatttttg
    49441 ctatttatct aaaaaagtga taactactta agatgaagca gaatatttac gccatacggc
    49501 atacaaccgc gaaaaatata aaaaaaagaa aacgattgaa cgcgtttttg cagtcgcaaa
    49561 ggaaaagcat ggaatgcgct acatcaagta tcgagacttg gaaaacaatc aggtgtatac
    49621 gctgattgtt tttgctgcaa tgaatctgaa aaagttgtcc acttggcagt tggaacatat
    49681 gtcttcatat cttttattat acaaaaatag caaaagcacc agtctacact cttagtaaag
    49741 aggaaactga tccttttgtc tacattctta aagtacttaa actaagtact ttattttact
    49801 ctttaggatt gatacagtag catctatccc tttattaata atcttttatt tttttaaata
    49861 aagatgttgt ctccatcttc aggcactaaa actttttcgg aaattccttt ttctttattg
    49921 aaagacttta attcttctct tgacagtccc caatggttta cagcttccat atgaacggca
    49981 ataattgtgg aatctggtat tgctttatgc aattcatata aatcgtcttt ccccataata
    50041 agagattctc caactaaaaa atgattatct ccagcatttg ctactatgat ttctggatta
    50101 tactgatcaa tagtttgttt aacagcttca taccaaactg tatctcctgt aaagtatagt
    50161 gtcttctcag tcgaatgttt gaaaacaata ccgcaaacat tacctacata attcaagatt
    50221 tctcctctgc catgtttgcc ttttgtttta accaattgta tagattcaaa aactgtgtct
    50281 tctcgtagta cctccacatt tttaaaacca tatagtcgaa tttcattcgc atcattttca
    50341 tcttgcacaa ataattttat ttctttaggt aggacctctt tcgctatatc gtcccaatga
    50401 tctagatgta aatgtgttac aataacagca tcaataccgt tgataatatc ttcgactggc
    50461 attggcaaat caactagagg attcatttga tcttttcttg gtgatggaaa aggacctttc
    50521 gaaataaatg gtggaagtgt tcctttttcc gctaacattg gatcaattaa aaatttttta
    50581 cctccatagt ctaagatagc tgttgcatta cgaatgtgat gtatattcat aaacgttgtc
    50641 tcctttttgt tgatatgaaa tatttttagt ataattcaat tccatacaat aaataaaccg
    50701 aacaaaaaaa gtcttttact atttttactt aatttatgaa aggttgttta ccatgttaga
    50761 ccaaacagat ataaatatca ttgaggaact atcaaaaaat agtcgaatca caatgaaaga
    50821 acttggagaa aaagtacatt tgactggacc cgctgtctcg gcgagagtga caaaactaga
    50881 ggaaagtgga gttattgaaa attatacagt aaaattaaat atggaaaagc tagggttttc
    50941 gattcacgct
//
LOCUS       DADVXO010000006        45496 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.006, whole genome shotgun sequence.
ACCESSION   DADVXO010000006 DADVXO010000000
VERSION     DADVXO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.006"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..833)
                     /locus_tag="KB950_000307"
     CDS             complement(<1..833)
                     /locus_tag="KB950_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6856550.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     Y"
     gene            complement(1057..2997)
                     /gene="thrS"
                     /locus_tag="KB950_000308"
     CDS             complement(1057..2997)
                     /gene="thrS"
                     /locus_tag="KB950_000308"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBB6856551.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3334..3513)
                     /locus_tag="KB950_000309"
     CDS             complement(3334..3513)
                     /locus_tag="KB950_000309"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBB6856552.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3560..5383)
                     /gene="cadA"
                     /locus_tag="KB950_000310"
     CDS             complement(3560..5383)
                     /gene="cadA"
                     /locus_tag="KB950_000310"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBB6856553.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5661..7940
                     /locus_tag="KB950_000311"
     CDS             5661..7940
                     /locus_tag="KB950_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6856554.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8033..8680)
                     /locus_tag="KB950_000312"
     CDS             complement(8033..8680)
                     /locus_tag="KB950_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856555.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8859..9578)
                     /locus_tag="KB950_000313"
     CDS             complement(8859..9578)
                     /locus_tag="KB950_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6856556.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9709..10860)
                     /locus_tag="KB950_000314"
     CDS             complement(9709..10860)
                     /locus_tag="KB950_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBB6856557.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11077..11817)
                     /locus_tag="KB950_000315"
     CDS             complement(11077..11817)
                     /locus_tag="KB950_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6856558.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11821..12144)
                     /gene="rsfS"
                     /locus_tag="KB950_000316"
     CDS             complement(11821..12144)
                     /gene="rsfS"
                     /locus_tag="KB950_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBB6856559.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12201..12794)
                     /gene="yqeK"
                     /locus_tag="KB950_000317"
     CDS             complement(12201..12794)
                     /gene="yqeK"
                     /locus_tag="KB950_000317"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBB6856560.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12778..13428)
                     /locus_tag="KB950_000318"
     CDS             complement(12778..13428)
                     /locus_tag="KB950_000318"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBB6856561.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13438..13749)
                     /gene="yhbY"
                     /locus_tag="KB950_000319"
     CDS             complement(13438..13749)
                     /gene="yhbY"
                     /locus_tag="KB950_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBB6856562.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13765..14874)
                     /gene="yqeH"
                     /locus_tag="KB950_000320"
     CDS             complement(13765..14874)
                     /gene="yqeH"
                     /locus_tag="KB950_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBB6856563.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14871..15398)
                     /locus_tag="KB950_000321"
     CDS             complement(14871..15398)
                     /locus_tag="KB950_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBB6856564.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15578..16363)
                     /locus_tag="KB950_000322"
     CDS             complement(15578..16363)
                     /locus_tag="KB950_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBB6856565.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16356..17225)
                     /locus_tag="KB950_000323"
     CDS             complement(16356..17225)
                     /locus_tag="KB950_000323"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBB6856566.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17226..18605)
                     /locus_tag="KB950_000324"
     CDS             complement(17226..18605)
                     /locus_tag="KB950_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBB6856567.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18613..19032)
                     /gene="fabZ"
                     /locus_tag="KB950_000325"
     CDS             complement(18613..19032)
                     /gene="fabZ"
                     /locus_tag="KB950_000325"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBB6856568.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19057..19533)
                     /gene="accB"
                     /locus_tag="KB950_000326"
     CDS             complement(19057..19533)
                     /gene="accB"
                     /locus_tag="KB950_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBB6856569.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19536..20771)
                     /gene="fabF"
                     /locus_tag="KB950_000327"
     CDS             complement(19536..20771)
                     /gene="fabF"
                     /locus_tag="KB950_000327"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBB6856570.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20790..21527)
                     /gene="fabG"
                     /locus_tag="KB950_000328"
     CDS             complement(20790..21527)
                     /gene="fabG"
                     /locus_tag="KB950_000328"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBB6856571.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21539..22456)
                     /gene="fabD"
                     /locus_tag="KB950_000329"
     CDS             complement(21539..22456)
                     /gene="fabD"
                     /locus_tag="KB950_000329"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB6856572.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22563..22787)
                     /locus_tag="KB950_000330"
     CDS             complement(22563..22787)
                     /locus_tag="KB950_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB6856573.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22840..23802)
                     /locus_tag="KB950_000331"
     CDS             complement(22840..23802)
                     /locus_tag="KB950_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBB6856574.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23812..24252)
                     /locus_tag="KB950_000332"
     CDS             complement(23812..24252)
                     /locus_tag="KB950_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6856575.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24558..25523
                     /locus_tag="KB950_000333"
     CDS             24558..25523
                     /locus_tag="KB950_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6856576.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25580..25810)
                     /locus_tag="KB950_000334"
     CDS             complement(25580..25810)
                     /locus_tag="KB950_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBB6856577.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25896..26783)
                     /locus_tag="KB950_000335"
     CDS             complement(25896..26783)
                     /locus_tag="KB950_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBB6856578.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26872..28095)
                     /locus_tag="KB950_000336"
     CDS             complement(26872..28095)
                     /locus_tag="KB950_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6856579.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28112..29158)
                     /locus_tag="KB950_000337"
     CDS             complement(28112..29158)
                     /locus_tag="KB950_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6856580.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29322..31049)
                     /gene="ptsP"
                     /locus_tag="KB950_000338"
     CDS             complement(29322..31049)
                     /gene="ptsP"
                     /locus_tag="KB950_000338"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBB6856581.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31049..31315)
                     /locus_tag="KB950_000339"
     CDS             complement(31049..31315)
                     /locus_tag="KB950_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBB6856582.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31794..34028
                     /locus_tag="KB950_000340"
     CDS             31794..34028
                     /locus_tag="KB950_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6856583.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34176..34496
                     /locus_tag="KB950_000341"
     CDS             34176..34496
                     /locus_tag="KB950_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBB6856584.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34701..34925)
                     /locus_tag="KB950_000342"
     CDS             complement(34701..34925)
                     /locus_tag="KB950_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBB6856585.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35123..36703)
                     /locus_tag="KB950_000343"
     CDS             complement(35123..36703)
                     /locus_tag="KB950_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBB6856586.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37104..38480)
                     /locus_tag="KB950_000344"
     CDS             complement(37104..38480)
                     /locus_tag="KB950_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB6856587.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38608..39726)
                     /locus_tag="KB950_000345"
     CDS             complement(38608..39726)
                     /locus_tag="KB950_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6856588.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39868..40302
                     /locus_tag="KB950_000346"
     CDS             39868..40302
                     /locus_tag="KB950_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6856589.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40380..40913)
                     /locus_tag="KB950_000347"
     CDS             complement(40380..40913)
                     /locus_tag="KB950_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBB6856590.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41007..42185)
                     /gene="mutY"
                     /locus_tag="KB950_000348"
     CDS             complement(41007..42185)
                     /gene="mutY"
                     /locus_tag="KB950_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBB6856591.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42178..42975)
                     /gene="recX"
                     /locus_tag="KB950_000349"
     CDS             complement(42178..42975)
                     /gene="recX"
                     /locus_tag="KB950_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBB6856592.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43082..44464
                     /gene="rlmD"
                     /locus_tag="KB950_000350"
     CDS             43082..44464
                     /gene="rlmD"
                     /locus_tag="KB950_000350"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBB6856593.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44638..45216)
                     /locus_tag="KB950_000351"
     CDS             complement(44638..45216)
                     /locus_tag="KB950_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBB6856594.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT        12981 a         9704 c         7901 g        14910 t
ORIGIN      
        1 aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta
       61 gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt
      121 gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc
      181 ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc
      241 gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc
      301 actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa
      361 atgattggct taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag
      421 gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac gactttttgg
      481 atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca
      541 agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg
      601 attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg
      661 atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca
      721 ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt
      781 tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct cataagttcc
      841 tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt
      901 atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc
      961 acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc
     1021 tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt
     1081 gacttctgct tccatagcat caataaagat attcaaatct tctacagctg tttctttact
     1141 tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg
     1201 gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc
     1261 atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc
     1321 gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg
     1381 aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat
     1441 tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa
     1501 atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc
     1561 aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc
     1621 ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt gggctttttc
     1681 ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg
     1741 gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg
     1801 tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat
     1861 ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc
     1921 taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat
     1981 catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat
     2041 tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac
     2101 atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat
     2161 ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga
     2221 acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct
     2281 ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc
     2341 tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc
     2401 cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca
     2461 caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact
     2521 aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag
     2581 aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc
     2641 cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga
     2701 atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc
     2761 catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat
     2821 gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac
     2881 tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt
     2941 tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat
     3001 agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc
     3061 tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa
     3121 cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc
     3181 gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta
     3241 tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac
     3301 taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc
     3361 gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt
     3421 gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg
     3481 accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc
     3541 ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg
     3601 ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca
     3661 aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac
     3721 aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca
     3781 ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac
     3841 tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg
     3901 gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt
     3961 ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt
     4021 tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca
     4081 catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa
     4141 ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt
     4201 ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta
     4261 ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt
     4321 ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag
     4381 ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt
     4441 ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga
     4501 gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc
     4561 ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta
     4621 cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag
     4681 caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt
     4741 cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt
     4801 cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag
     4861 gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc
     4921 ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat
     4981 caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa
     5041 ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat
     5101 cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt
     5161 tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg
     5221 aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg
     5281 cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc
     5341 caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt
     5401 tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt
     5461 ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg
     5521 tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc
     5581 atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg
     5641 gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa
     5701 cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc
     5761 aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg
     5821 aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat
     5881 accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc
     5941 cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta
     6001 tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg
     6061 ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa
     6121 tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta
     6181 tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat
     6241 caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc
     6301 gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg
     6361 ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa
     6421 acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt
     6481 cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc
     6541 cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag
     6601 atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc
     6661 aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc
     6721 tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc
     6781 atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg
     6841 aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa
     6901 cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca
     6961 accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg
     7021 tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac
     7081 gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc
     7141 gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat
     7201 atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg
     7261 caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg
     7321 gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt
     7381 tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa
     7441 tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa
     7501 aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag
     7561 cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta
     7621 aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag
     7681 tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct
     7741 ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt
     7801 tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat
     7861 tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt
     7921 atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct
     7981 ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta
     8041 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
     8101 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
     8161 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
     8221 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
     8281 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
     8341 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
     8401 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
     8461 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
     8521 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
     8581 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
     8641 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
     8701 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
     8761 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
     8821 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
     8881 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
     8941 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
     9001 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     9061 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     9121 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     9181 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     9241 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     9301 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     9361 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     9421 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     9481 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     9541 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     9601 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     9661 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     9721 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     9781 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     9841 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     9901 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
     9961 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
    10021 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
    10081 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
    10141 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
    10201 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
    10261 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
    10321 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
    10381 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
    10441 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
    10501 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
    10561 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
    10621 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
    10681 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
    10741 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
    10801 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
    10861 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
    10921 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
    10981 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
    11041 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
    11101 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
    11161 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
    11221 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
    11281 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
    11341 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
    11401 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
    11461 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
    11521 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
    11581 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
    11641 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
    11701 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
    11761 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
    11821 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
    11881 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
    11941 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
    12001 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
    12061 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
    12121 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
    12181 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
    12241 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
    12301 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
    12361 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
    12421 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
    12481 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
    12541 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
    12601 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
    12661 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
    12721 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
    12781 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
    12841 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
    12901 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
    12961 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
    13021 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
    13081 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
    13141 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
    13201 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
    13261 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
    13321 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
    13381 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
    13441 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
    13501 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
    13561 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
    13621 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
    13681 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
    13741 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
    13801 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
    13861 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
    13921 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
    13981 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
    14041 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
    14101 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
    14161 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
    14221 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
    14281 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
    14341 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
    14401 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
    14461 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
    14521 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
    14581 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
    14641 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
    14701 caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac
    14761 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
    14821 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
    14881 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
    14941 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
    15001 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
    15061 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
    15121 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
    15181 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
    15241 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
    15301 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
    15361 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
    15421 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
    15481 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
    15541 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
    15601 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
    15661 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
    15721 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
    15781 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
    15841 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
    15901 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
    15961 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
    16021 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
    16081 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
    16141 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
    16201 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
    16261 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
    16321 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
    16381 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
    16441 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
    16501 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
    16561 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
    16621 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
    16681 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
    16741 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
    16801 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
    16861 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
    16921 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
    16981 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
    17041 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
    17101 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
    17161 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
    17221 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
    17281 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
    17341 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
    17401 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
    17461 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
    17521 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
    17581 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
    17641 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
    17701 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
    17761 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
    17821 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
    17881 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
    17941 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
    18001 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    18061 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    18121 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    18181 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    18241 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    18301 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    18361 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    18421 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    18481 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    18541 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    18601 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    18661 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    18721 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    18781 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    18841 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    18901 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    18961 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    19021 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    19081 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    19141 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    19201 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    19261 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    19321 tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac
    19381 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    19441 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    19501 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    19561 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    19621 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    19681 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    19741 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    19801 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    19861 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    19921 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    19981 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    20041 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    20101 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    20161 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    20221 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    20281 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    20341 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    20401 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    20461 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    20521 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    20581 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    20641 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    20701 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    20761 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    20821 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    20881 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    20941 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    21001 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    21061 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    21121 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    21181 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    21241 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    21301 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    21361 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    21421 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    21481 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    21541 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    21601 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    21661 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    21721 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    21781 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    21841 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    21901 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    21961 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    22021 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    22081 cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    22141 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    22201 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    22261 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    22321 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    22381 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    22441 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    22501 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    22561 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    22621 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    22681 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    22741 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    22801 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    22861 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    22921 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    22981 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    23041 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    23101 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    23161 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    23221 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    23281 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    23341 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    23401 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    23461 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    23521 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    23581 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    23641 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    23701 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    23761 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    23821 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    23881 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    23941 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    24001 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    24061 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    24121 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    24181 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    24241 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    24301 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    24361 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatttctttt
    24421 attagaaaaa tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac
    24481 tatagaattc cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa
    24541 gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg
    24601 tctatcttat gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca
    24661 aaaatagagt aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta
    24721 tgattcatca tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat
    24781 tgctcgagtg tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt
    24841 tcagtaatta taattatgta catattacca ataagggatt tgtattaaca gatactccta
    24901 cggctcgaac atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa
    24961 tggaagaaac ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc
    25021 agcttcctga cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt
    25081 ttcaaggccg aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct
    25141 atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt
    25201 ttattagacg gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca
    25261 tgccattagc agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg
    25321 cagaaacaat gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag
    25381 ctcatttatc tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga
    25441 tcagttaccg caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag
    25501 aagctgaaaa gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa
    25561 atgtcaatta tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga
    25621 tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg
    25681 ctttatcata agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca
    25741 gttcattatc tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg
    25801 aatgtgtcat cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc
    25861 tacttgcatt gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt
    25921 ccttcaggcc accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct
    25981 gccaactctt catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt
    26041 tctgcttctt ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt
    26101 gtgtaatctt cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt
    26161 ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca
    26221 agcatttccg tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta
    26281 tgctgacctt tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa
    26341 ggcagtactt gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc
    26401 tttgctccta agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt
    26461 gctgtttttg caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata
    26521 tgtcctgaga tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct
    26581 ttggggtaac ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta
    26641 tctgcccaga tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag
    26701 acagctactt cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct
    26761 gttccgataa aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct
    26821 atgctagtat acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt
    26881 aaatgaattt aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat
    26941 ctcttcttca tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg
    27001 tgtagatgaa atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact
    27061 ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa
    27121 tgaaggatga tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc
    27181 ggctgccatc gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa
    27241 ataatctgcc gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg
    27301 cacaaattct tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc
    27361 ttttcctacg ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt
    27421 aatcaaggct tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg
    27481 gttttcctga agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga
    27541 acgtttgaat ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc
    27601 atagctgcgt aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt
    27661 tgcaaataat ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat
    27721 ataatgaagg tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt
    27781 cttagctacc atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc
    27841 tagttctagt tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg
    27901 atccttgaaa gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc
    27961 cgcattagga tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg
    28021 ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa
    28081 aaagccgatt ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta
    28141 atttgctaga gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga
    28201 ttttattgat acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt
    28261 cttgattggt aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc
    28321 agttcttgct atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt
    28381 gtttgcttgc taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa
    28441 agaatagatc tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa
    28501 acaggacatt ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt
    28561 acatgggaac atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta
    28621 cgaaatcaat gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat
    28681 ccggagacag tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt
    28741 tctctaagtc gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca
    28801 acaactgttt tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta
    28861 ccagatattt ttttacgata ggagaaagct ggttcgatct gataaaggaa tttcggaaat
    28921 cttcctctgt agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga
    28981 ccatattatg actattgatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt
    29041 catctagtgt ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac
    29101 gcccgattcc agattttgca agcatctttt cactttcaaa atataataat accttcacct
    29161 tacgtccccc ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc
    29221 cctatttttg tctattctta ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg
    29281 cgtagctctt ttacaagcca caccagcttt tgatacgttg tttattttac atattcatga
    29341 acaagttcaa cgacttcttc catcatatcg cattctttca atgcacgatc agcaagttca
    29401 gccattttcg ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat agatgtcgca
    29461 ctcattgaga actcatctag acccatacct actagcaatg gaacagctgt ttgatctcca
    29521 gccatttctc cacacatacc tgcccatttt ccttcagcgt gtgctgcatc aattacattt
    29581 ttgatcaaac gtaaaattga tgggttatat ggttggtaca agtaagaaac gcgttcgttc
    29641 atacggtctg ctgccattgt gtattgaatc aaatcgttcg tacctacaga gaagaaatct
    29701 acttcttttg caaacttgtc tgctaagact gctgctgctg gaatctcgat catgatgccg
    29761 acttggatcg tatcagatac ttccttgcct tcacttatca atttttgttt ttcttcttca
    29821 aagatcgctt ttgctgcgcg gaattctttg agtgtagcta ccatagggaa catgatacgt
    29881 aaattaccat gaacagatgc acgaagcaat gcacgcattt gtgtacggaa catttcgtcg
    29941 ccttgttcag ataaactgat acgcaatgca cgatatccta agaatggatt catttcatga
    30001 ggtaattgca gatatggtag ttctttatct ccaccgatgt ccattgtacg aacaactaca
    30061 ggttttcctt ccataccttc taatacagct ttgtaagctt catattggtc atcttctgtt
    30121 gggaagtctg gagaatccat atacaggaat tcagtacggt acagaccaac tgcttctcca
    30181 ccattattat ggacaccaac tagatctttt ggtgttccga tgtttgcagc taattcaaaa
    30241 tgcttgccgt cagctgtgac agtttctgca ttttttaatt tttcccattc tgctttttgt
    30301 gctgcgtaat ctgcacctgc tttttcaaat tcagcttttt gttcatctgt aggatcgatg
    30361 attacatcgc cttcgatccc gttgacagcc aaaatatcgc cttctttgac tttagcagta
    30421 atttcttttg tcccaacgat tgctggaatt tctaaagaac gtgccatgat agcagaatgt
    30481 gaagtacgtc caccaatgtc tgttacaaac gcttttacaa aattgcgatc taattgtgct
    30541 gtatcacttg gagtcaagtc atgtgcaaca acgaccactt cttcgttgat cattgatgga
    30601 tttggtaaag taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc acgaatatct
    30661 gcagcacgct cttgcatata agcattatct tccattgctt cgaacatacc gatatacata
    30721 tccgttactt cttttagcgc tgattcagca tttacgctgt tgtctttaat attttgcttg
    30781 atttgaccta ccatttctgg atctgaaaga accatcaaat gggcatcaaa tacttgcgct
    30841 tctgcttcac ctaagctttg cgctgctttt tcacgaattt gctgcaattc ttcagtagat
    30901 ttagctaatg cagcatccaa acgagcttct tcagccgcag tatcttctac tgttgcctta
    30961 ctgaatgtta aatccggttg aactagcagg taagcttttg caacggctac accgtcacta
    31021 gcggcaatcc cttttagcat ttcaaccatt attcagataa gccttctttc ttcattgttt
    31081 caacgattgc tgccattgca tcagcttcgt cagcaccttc tgcagtaata gtaacatcag
    31141 aaccttgacc tacacctaaa gacataacgc ccatgattga tttcaagttt actgatttac
    31201 ctttatactc taagtttaca tcagagttaa atttgcttgc tgtttgtact aatagtgtag
    31261 ctggacgagc gtggatccca gtttctgcta ctacgtgaaa ttctttcttt tccatattaa
    31321 tcggtctcct ttaagtgagt gttgttttta tgtgagggtt ttctttttta gtaattcaga
    31381 gaatcctaac aaatgccacc ctttcatctg taaagattaa catttttttt cgttagatac
    31441 aacagttttc attcaatttc agataaaagt attcaaatta ttccggattc tttttcatcc
    31501 ttttcttcac attttataac aaacgtgttt atttcctttt tcttgtcgct ttctcttctc
    31561 ctgacgttta tcattctttc ctttgaccct ctttgttttc atatgaaacg aattacttgc
    31621 ttcccttttt atttttagta taataagagt caaaggtcag gaatggtcaa tagagatttc
    31681 accttttcta cctcataaat ctcttaaaaa actaggggtt attcttttac ctcatgaaaa
    31741 acgagatgta aaatatggat gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt
    31801 gtcaaaactg cggcaagaat gaggcaacga ttcacttgta tgccactgtc aatggtcagc
    31861 ggacacaact cgattactgt cagagctgtt atcaaaaaat caaaaatcaa caaaatggag
    31921 gtctgactaa tatggcccaa aatgatccat ttggatttgg cagtttggac gatcttttcc
    31981 gttccatgtc tcgccaaatg caacagcaag gaaattatga gcaaacacca ccaacacaat
    32041 ttggcggaaa caacaacttc aatggtggtc agccacctca aggcaataca gaaggtttat
    32101 tgggagaata cggaatcaat atcacagaac aagcacgaca aggtgatatc gatcctgtga
    32161 tcggacgcga tgacgaaatc aaacgagtca ttgaaatttt aaatcgccga accaagaaca
    32221 atcctgtctt gattggagaa ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac
    32281 aaaaaatcgt agatggtgat gtaccgcaaa aattaatgga caaagaagtc attcgtttag
    32341 atgtcgtatc tttagtccaa ggtactggta tacgaggaca atttgaagaa cgtatgcaaa
    32401 aattgattga agagatccgt caagcagaaa atgtgatcct attcatagat gaagttcacg
    32461 aaatcgtcgg tgctggttct gctggtgatg gaaatatgga tgctggtaat attttaaaac
    32521 cagctttagc tcgaggagaa ttgcaaatgg ttggtgctac taccttaaat gagtaccgca
    32581 tcatcgaaaa agacgctgct ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa
    32641 ctgtcgaaga aacgatcagt attttgaaag gtttgcaaaa acgttatgaa gactatcatc
    32701 atgtaaaata tactgatgag gccatcgaag cagccgcaac tttatccaat cgttacgtcc
    32761 aagatcgctt cttgccggat aaagccatcg atttgctaga tgaaacaggt tctaagaaaa
    32821 acttgacgat ccaaatcgtt gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag
    32881 aacaaaaagt tttagcttct cgagaagaag atttcgaaaa agcagcttat tatcgcgatc
    32941 agatcaataa acttcaaaaa atgaaagaac gtcagctcac tgaagaggaa acacctgtga
    33001 ttaccgaaaa agatatggaa aaaatcgttg aacaacggac aggtattcca gtgggtgaat
    33061 taaaagaaaa agaacagaca caactgaaaa acttagcaga tgacttgaaa gctcatgtca
    33121 tcggacaaga taacgctgta gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat
    33181 taaacaaaca gaatcgtcca atcggctcct tcttatttgt tggaccaact ggtgtaggta
    33241 aaacagagct tgcgaaacaa cttgcctacg aactattcgg ttcacaagat tcaatgattc
    33301 gtttcgatat gtctgaatat atggaaaaac atagtgtctc taaattgatt ggttctcctc
    33361 caggttatgt cggatacgaa gaagctggtc aattaacaga aaaagtacgg agaaatcctt
    33421 acagtcttgt cttgttagat gaagtagaaa aagctcatcc agatgttttg catatgttct
    33481 tgcaaatctt agatgatggt cgattgacag atgcacaagg aagaacggtc agctttaaag
    33541 atacgatcat cattatgaca agtaatgccg gaactggtaa agtagaagca aacgttggtt
    33601 ttggtgcggc acgcgaaggt gttactcgtt ctgtattgaa ccaattaaac aactacttta
    33661 caccagaatt tctgaaccgt tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa
    33721 acttgatgac aatcgtcaca ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc
    33781 aacttcacat cgaagttcct acgaatgtca aagaaaaatt agttgactta ggctatgatc
    33841 catctatggg tgctcgtcct cttcgacgca cgatccaaga acaaatcgaa gatggtatcg
    33901 ctgaatttta tcttgatcac ccttctatcc atgagttgaa agcaaaactg gataaagatg
    33961 gaaaaatcat cgttacttct aaaccagaac gtttggctaa agaatcagcc gaagaaacgg
    34021 ctgaataaat ccagttttat agcgtgagac ggtatttctt tgagaaatgc cgtctcattc
    34081 ttttttcaga aaagtcaaaa tactgttcat tttataaaaa agaaattata ataagagaaa
    34141 caagcaatta aaaatacata aaagaggtga gcgccatgaa attggtaaat gtaacaaaca
    34201 gccattcccg tcttgtatta aaccagcttg aaaacactga tgcccatcta gtcaaggtct
    34261 acacagccgg aaataccact attgtctaca ctgaagcccc tgaacataat gaaattctgc
    34321 tgatcaatga caaacgtaaa atccagccga aagaaatcga agatgctaaa aattatttct
    34381 tagataaaat caaagacgcc gtttaccacg aggatgaaat caaagtaatc gaattggcag
    34441 gccttgttga aatctcgatt ccaaaacatg aattaggcgc tacattagcc gtgtaaacaa
    34501 attttgaaca taataattaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    34561 tctaaacacg tataaacggc gaaagcagaa gcaactcctt cggaaataag ctgaaattca
    34621 cgaaaattgc ttttcaaatt tttgtgaatt ccagcttatt tcttggagtt aaacgcttct
    34681 gtctcagcct ctttttcttt ctagcttttt gatttttctc ttttcttttg tacttttctc
    34741 ttttcatttt cagttctacg cgtgtcttta tttttaactt tttcctcttt atggatgatt
    34801 gatcgtactt tttgtgcaat ttgctcgttt ttgtcccagt cttttacatc taataatcga
    34861 cataatcgta acatctcttc ttttttctgt ttttttcttt cacgtctttg ccattctgtt
    34921 tccatgtgtc acactctcct ttctttaatc atatgtacgc aaaataattt ttatattttt
    34981 ttgattagac atttctatga ttttaatata gtaaaaagag ccgtcacgga aaatccgtca
    35041 cggctctaaa caaaatatga gaggctgttt ttgtttggct attcttatta cttgatgcag
    35101 ggcagcttgt gtcaagactt ctttacatca aactttttaa ttttacattg ggatatttat
    35161 ccgcaaacca gcgttctgca aattggttct cgaataagaa caacggttgg tcgaaacggt
    35221 cacgagccaa gatattgcga cttgaactca tcttctcatc caaatcttct ggatcgatcc
    35281 agcgagcgat tttgtgtccc attggagaca tgattacttc ggcgttatac tcatttaaca
    35341 tacgatattg gaatacttcg aattgtagct gaccaacagc tccaatgata tattcttccg
    35401 ttagatatgt tttataaagc tgaatcgctc cttcttgaac taattgatag attcctttgt
    35461 ggaaggattt ttgtttcatg acattttttg cagtcacttt cataaacagc tctggagtaa
    35521 atgaagggag ttcttcataa gcaactttta gttttccttc gaacaatgta tccccaatct
    35581 gataattgcc tgtatcatat acaccgatga tatcgcctgc tactgctttt tcgacgtttt
    35641 ctctcgaatc tgccataaat tgtgtgacat tgcttaattt cagtttttta tttgtacgtt
    35701 ccaaccagac atccattcct cgttcgaatg ttccagaaca gattcggaca aaagcgattc
    35761 ggtcacgatg ggctggattc atatttgctt ggatcttgaa tacgaaacct gaaaattctt
    35821 cctcataagg acttaccatc tgatcatctt gtgttttgtg tccataagga gctggtgcca
    35881 attcaacgaa agtctctaaa aaagtctgta caccgaagtt agtcaatgca gaaccaaaaa
    35941 atactggtgt ctgatcgcca cgagcaattt tttcttgatt aaatgcatct ccagcctctt
    36001 tgattagttc tacttcttct aagacttgtt catataagga attttcagtt agaggatgat
    36061 tttcgggaag cagtccatct tcatccaatt ctactaatcg ttcatcctga taattttccg
    36121 gacgataaaa ctctacacga ttatggtaga tatcataaag accttctagc cctttaccca
    36181 taccgattgg ccaattcatc ggataagatt cgatatctaa tagctcttct aattcttcta
    36241 acaattccaa tggttcacga ccatcacgat ctagtttgtt gataaaagta aatatcggga
    36301 tgccacgttt ttttacaact tggaataatt tctttgtctg tgcttcgatc ccttttgcac
    36361 tatcgattac catgaccgca ctatccacgg ccatcaaagt acgataagta tcttcagaaa
    36421 aatcttcatg tcctggtgta tccaagatat ttactcgttt cccttgataa tcaaactgca
    36481 taacagaact agtaacagaa atcccacgtt gtttctcgat ttccatccag tctgatttgg
    36541 caaaattacc tgtttttttg ccttttacgg tcccggcttg acggatagcc ccaccaaaca
    36601 atagtaattg ttctgtaatc gttgttttcc cagcatccgg atgggaaatg atcgcaaaag
    36661 ttctgcgatt gtccacttgt tgctttaatt ttggattatt cataacctaa ctccatttct
    36721 tccattcaat tatttatatt ttctctaggc acagattgtc cgatctctta aagaaagaca
    36781 atttcatata gggctgtgac aaaagccgtg tagcagtctc tttttccgag taaacggcgt
    36841 tctagagaca gcctatcagc aataagccca cccaagccaa aaatacgaaa agctgttttt
    36901 gcttggctgc tcttattact taatgcagga tagctttttc acaacctttg tcaactacta
    36961 caactgaatc agtatagcat ggattcagcg aatgaaaaag ccaccaaacc ataagagtca
    37021 aatatttttt tgtcagctga ctaaaagaaa agacgtgttg cttgtttatt caagcaacac
    37081 gtctttcata accaagtttc caattatctt cttggctcgt catcttcgaa ttcttttctg
    37141 aagtatcttc gttcttcttc aggttccgct tctgtttctt ccggatcatg aaaaatgaca
    37201 cggattttaa ggattcgaga accttccatt tcttcagaaa ccagcgtaat attatcgaca
    37261 tcaaacgata acttttcgcc ttcatccgga atcatgccta atgctgtaat caaataacca
    37321 gccatcgtat ctacatcact catatgcaga tcactgtcaa aagcttcatt gaactcatca
    37381 atcagcatac gtccttggat gatatattcg tgttcatcga ttttttcata aagattctca
    37441 acttcatctg attcatcatc gatttctccg acgatttctt ctaaaaggtc ttctagtgtt
    37501 gctaacccaa cgacgccgcc atattcatct aataaaatag ccatctgatt ctgcgtcttt
    37561 ttcatttcat ataacagatc atcaataaag accgtttcag gaacgaataa aggctcctgc
    37621 atgattttct tgatatctaa attatcaaag ccgaatttat gggcagcttt tagtaaattc
    37681 tttgtatgaa ggattccgac tactttatct ttgtcttcgt tataaacagg gattcttgaa
    37741 tagttttcgg ataaaacttc attcacattt tcttccacag tgtcgttgat atcaatcata
    37801 aatgcatccg ttcgtgggac catgacttct cgggcaactt tcgtatcaag agaaaaaacc
    37861 ccttggagca tttctaattc ttcattattc aatacgcctt cgttttcaag catataacgc
    37921 atttcatctc tggtcatttt tgaatcttca tcatcaaaag tcatcggcgt gatacgcgcc
    37981 aataaacttg tggaagcaga aagcagccag acgaagggct tagctactac accaatcaag
    38041 cgtatcagcc ctgaagtgaa ctttgcgact tcttctgatt tatttaaagc aatacgttta
    38101 gggtacaatt ctccaaaaac aatcgataca taggtcaaaa tagccagcac aatgatattt
    38161 gcaatatttt ttgccgctgt gccgccacct aataccggag ctaaccgttc cgataaagta
    38221 ttagctagtg aagcaccaga caaaatgttg accaaagtga ttcctacttg gattgtcgat
    38281 aggaaattat ttggattctg caatattttt aaaagctttt gagatttcac atctccttca
    38341 tcagcttttt gctcgattcg attcttattg atcgaaacaa cagcaatttc ggaagctgct
    38401 aaaaaagcat tgatcaatgt caaaacgatc aataataaaa tctgcgctaa cagcgactga
    38461 ctctcagggt cagcattcat aatcgcattc tctccttata aaattaattt ttagtttacg
    38521 atagaaaaaa acgtggcact atagccacgt taaaatggta tcacaaagca attttctttg
    38581 caataaaaac aagtgtacta aaactaccta cgaggtcgta gcaattttct tcttgttttc
    38641 attttgcttg tcttctttga cgattttgat tatataggaa atgatcgtct tgcagattgc
    38701 gtaaaaagga acacctaaga agataccaag caatccggca atatttccag ctactagaag
    38761 aacgataatg atcgttaatg gatggatttt cagtgatttc ccaattacat taggataaat
    38821 gatattccca tccaactgtt ggacgattag cacaaccaca caacaaagta atgcttttac
    38881 cggatcattg aagactgtta ctagaaatgc aggtgctagt cctagataag gtcctaaata
    38941 tggaattagg tttgtcaatc ccgcaatgac cccaaataag aaagcataat ccacaccaat
    39001 taccatatat cctaaaaatg tgaaggtacc aacgaataaa cattcgattg cttgtccgct
    39061 aatgtaatta gccaatgttt tattcagttg ccccaataac tccacaattt ggtttcttct
    39121 tttttctggg aaaaactgtt ggatgtttgg cactagtttt tctccgtctt tcaacatata
    39181 gaacaagatg aatggtgctg taatgattac tacggttgcc gaagcgatcg ttccaacgat
    39241 cgaacctaaa ctattggata ctccactaag gaattgctga atgattgtcc cgtaagaaat
    39301 gtctaattgc tcaaaatatt tattgatatc tacttccttg aacaaaggca attcggctac
    39361 ctcttttaac cagctttcta cctgtttgat gaaatcaggc atattagatg ccaatgagga
    39421 aatttgctgt actagacttg ggatgacact aagtaaaatc caaataattg ctgcaactaa
    39481 aaggagcagc acaattgcga cagcccatat tcttttgatt tttgctttca tcaacagctt
    39541 gacaatagga ttcaataaat agtaaagaaa gcctgcaatc agtacaggtg caaataacgt
    39601 ggaaaaaaat gtcccgatcg gttcgaaaat aaagtttatt tctgatgaaa tcatgatcaa
    39661 tgcagctatg atcaataatt caagcgacca aaacattagt ttagactgtc taagtttttc
    39721 aaacaataga tttcctcctt tctttcttac tagtatatat tatattcgtt aaaaattctt
    39781 ttattctttt tttatttttg aaaacttgcc atacttttta tttgttttat tttccagtat
    39841 actatacgaa aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga tacaatgagc
    39901 aatatttact tagatgctgt acggatcagg catcaagttt ttgtagtcga acagggtgtg
    39961 cctttgtctc gagaaataga caaagatgaa gcccattgta tccacttcgt cctgtactca
    40021 gataaaaaag aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa
    40081 ttacagcgga tggcgattct ttctgaatac agacaccaag ggttaggaaa aatattgatt
    40141 gaagaagcag aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag
    40201 tccacagctg aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa
    40261 gatgctggca tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa
    40321 gcaaaaaaca gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt
    40381 tacttacgcg acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac
    40441 gggccatctc cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag
    40501 tcaatatcgt tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat
    40561 agtcgtttct cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag
    40621 gcctcatcgt ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct
    40681 ctgatcattg ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg
    40741 cgcgttaccc atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc
    40801 atagagtgtt cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg
    40861 tcgtgcttgt aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc
    40921 cttccttaag cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt
    40981 attatatcaa taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct
    41041 aactgatcta ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc
    41101 ttcaaccact tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt
    41161 ccgtaaaaaa ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa
    41221 tgtcgtgtct gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct
    41281 gctactaggt catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc
    41341 tcatactcct cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt
    41401 tggctatctc tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata
    41461 taataaacgt cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct
    41521 cgtttgttag ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt
    41581 gtacagatag ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca
    41641 tcaatgattt tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct
    41701 gcttcaatgc aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct
    41761 ggaagtccaa atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat
    41821 gagctaattt cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta
    41881 gcagcagctt ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa
    41941 agcttttctt ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga
    42001 taaaaataat cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag
    42061 atacgataag gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat
    42121 tgaatgaact gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta
    42181 ttcactttcc tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt
    42241 ttgatataag ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct
    42301 tcgccatagt ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc
    42361 ttgcggaaga tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa
    42421 tgtttgttgg atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc
    42481 tttttcggct gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc
    42541 ttgttcaatg atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg
    42601 tcctttgtca cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc
    42661 gacaaggtcc aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc
    42721 cttttctttt aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt
    42781 cattgctagg tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc
    42841 ttcgtctaat tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg
    42901 taaagcttct ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct
    42961 cgtaacggtt aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat
    43021 ccccgtaatc aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact
    43081 tatggcagaa acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg
    43141 cataaatggt gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact
    43201 accaaaagaa gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga
    43261 attaataaaa gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta
    43321 tgaatgcggt ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa
    43381 ggatctgctt ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact
    43441 tttgccgacg atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt
    43501 acgaaaaaat aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca
    43561 gttagtccct ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac
    43621 tgttgttcaa ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg
    43681 aatcttccgc acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt
    43741 tattactcgc agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg
    43801 actgcccgaa gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat
    43861 gggggatgaa acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt
    43921 gactttcgat ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct
    43981 ttatggcgaa ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta
    44041 ttgcggagtt gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat
    44101 ggataccgtt cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca
    44161 caacactcga tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg
    44221 ttttcagcct gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat
    44281 ccaatcatta ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac
    44341 acttgccaga gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt
    44401 cgacatgttt cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga
    44461 ctaaatgaaa gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca
    44521 aaaatagctc ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac
    44581 actatttatt taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta
    44641 ttggatattc acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac
    44701 tgctaaaatc acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt
    44761 ttttggtgga acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt
    44821 caaaggttga ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg
    44881 atgttgtgct gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg
    44941 ccatctgtta gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat
    45001 ttttccatca atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct
    45061 tccgacattt tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg
    45121 agaagtaata taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac
    45181 taacccaata aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa
    45241 ccacattgta aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg
    45301 aagtaaaaaa ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac
    45361 aaatagaaaa catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac
    45421 aaatgttcaa ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat
    45481 acaggtcttg ctgtga
//
LOCUS       DADVXO010000007        43073 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.007, whole genome shotgun sequence.
ACCESSION   DADVXO010000007 DADVXO010000000
VERSION     DADVXO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.007"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="KB950_000352"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="KB950_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBB6856595.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="KB950_000353"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="KB950_000353"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBB6856596.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="KB950_000354"
     CDS             complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="KB950_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBB6856597.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="KB950_000355"
     CDS             2179..2499
                     /locus_tag="KB950_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBB6856598.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="KB950_000356"
     CDS             complement(2611..3480)
                     /locus_tag="KB950_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6856599.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="KB950_000357"
     CDS             complement(3576..4880)
                     /locus_tag="KB950_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6856600.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="KB950_000358"
     CDS             complement(4877..5386)
                     /locus_tag="KB950_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856601.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="KB950_000359"
     CDS             complement(5561..7696)
                     /locus_tag="KB950_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6856602.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="KB950_000360"
     CDS             complement(7779..9434)
                     /locus_tag="KB950_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6856603.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="KB950_000361"
     CDS             complement(9436..9750)
                     /locus_tag="KB950_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6856604.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="KB950_000362"
     CDS             9920..10180
                     /locus_tag="KB950_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBB6856605.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="KB950_000363"
     CDS             10194..10508
                     /locus_tag="KB950_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBB6856606.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="KB950_000364"
     CDS             complement(11238..11792)
                     /locus_tag="KB950_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBB6856607.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="KB950_000365"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="KB950_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="KB950_000366"
     CDS             complement(14153..14869)
                     /locus_tag="KB950_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBB6856608.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="KB950_000367"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="KB950_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBB6856609.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="KB950_000368"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="KB950_000368"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBB6856610.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="KB950_000369"
     CDS             complement(18339..19799)
                     /locus_tag="KB950_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6856611.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="KB950_000370"
     CDS             complement(19789..21084)
                     /locus_tag="KB950_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6856612.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="KB950_000371"
     CDS             complement(21416..22093)
                     /locus_tag="KB950_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBB6856613.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="KB950_000372"
     CDS             complement(22123..23214)
                     /locus_tag="KB950_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBB6856614.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="KB950_000373"
     CDS             complement(23315..23749)
                     /locus_tag="KB950_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6856615.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="KB950_000374"
     CDS             complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="KB950_000374"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6856616.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EWVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="KB950_000375"
     CDS             complement(25787..27682)
                     /locus_tag="KB950_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6856617.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="KB950_000376"
     CDS             complement(27740..29515)
                     /locus_tag="KB950_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBB6856618.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="KB950_000377"
     CDS             complement(29565..31061)
                     /locus_tag="KB950_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6856619.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="KB950_000378"
     CDS             complement(31255..32877)
                     /locus_tag="KB950_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBB6856620.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="KB950_000379"
     CDS             complement(33205..34518)
                     /locus_tag="KB950_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBB6856621.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="KB950_000380"
     CDS             complement(34763..34987)
                     /locus_tag="KB950_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856622.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="KB950_000381"
     CDS             complement(35007..35429)
                     /locus_tag="KB950_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBB6856623.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="KB950_000382"
     CDS             complement(35571..36281)
                     /locus_tag="KB950_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856624.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="KB950_000383"
     CDS             complement(36299..36796)
                     /locus_tag="KB950_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6856625.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="KB950_000384"
     CDS             complement(36833..37285)
                     /locus_tag="KB950_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6856626.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="KB950_000385"
     CDS             complement(37420..38025)
                     /locus_tag="KB950_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6856627.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="KB950_000386"
     CDS             complement(38475..39095)
                     /locus_tag="KB950_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6856628.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="KB950_000387"
     CDS             complement(39097..39966)
                     /locus_tag="KB950_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBB6856629.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="KB950_000388"
     CDS             complement(39989..40396)
                     /locus_tag="KB950_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6856630.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="KB950_000389"
     CDS             complement(40415..41704)
                     /locus_tag="KB950_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6856631.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            41762..>43073
                     /locus_tag="KB950_000390"
     CDS             join(41762..42203,42203..>43073)
                     /locus_tag="KB950_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBB6856632.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPV"
BASE COUNT        12330 a         9160 c         7260 g        14323 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 acccattccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgttttg atgtgcaccc caaaagttag actagaaatc taattaaaag gggtgctttt
    41761 tttgcggaaa tatacatttg tttttaagaa aaaagtagtt tcagactatt taaataacga
    41821 aggcggctac aaatatcttg cacataaata tcaaataaat cgtacactgg ttagacattg
    41881 ggtaaggatt tataactatc atggttggga aggcttggtt ggaggtggca aaagctacac
    41941 tacaaaattt aaacttgatg ttatagaata tatggaaaca aatggtcttt ctatccaaga
    42001 aactgctaaa aaatttaata tcggttcaaa tagaactcta agtaaatgga tagagcaata
    42061 tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg ggcagaaaaa ttagtatgaa
    42121 ttccaagcta aacattccta aaaaacttaa agatgagtct cttgaagaag aagttattcg
    42181 tttaagagca gagaacgcat atttaaaaaa gttagaaacc ttgattcaag aacaagattt
    42241 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
    42301 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
    42361 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
    42421 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
    42481 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
    42541 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
    42601 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
    42661 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
    42721 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
    42781 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
    42841 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
    42901 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
    42961 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
    43021 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agt
//
LOCUS       DADVXO010000008        42777 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.008, whole genome shotgun sequence.
ACCESSION   DADVXO010000008 DADVXO010000000
VERSION     DADVXO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.008"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            142..789
                     /locus_tag="KB950_000391"
     CDS             142..789
                     /locus_tag="KB950_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBB6856633.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            804..2330
                     /locus_tag="KB950_000392"
     CDS             804..2330
                     /locus_tag="KB950_000392"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBB6856634.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2379..5177)
                     /gene="ileS"
                     /locus_tag="KB950_000393"
     CDS             complement(2379..5177)
                     /gene="ileS"
                     /locus_tag="KB950_000393"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBB6856635.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5439..6146)
                     /locus_tag="KB950_000394"
     CDS             complement(5439..6146)
                     /locus_tag="KB950_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBB6856636.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6167..6949)
                     /locus_tag="KB950_000395"
     CDS             complement(6167..6949)
                     /locus_tag="KB950_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBB6856637.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(6998..7285)
                     /locus_tag="KB950_000396"
     CDS             complement(6998..7285)
                     /locus_tag="KB950_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBB6856638.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7300..7899)
                     /locus_tag="KB950_000397"
     CDS             complement(7300..7899)
                     /locus_tag="KB950_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBB6856639.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7913..8590)
                     /locus_tag="KB950_000398"
     CDS             complement(7913..8590)
                     /locus_tag="KB950_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBB6856640.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8607..9848)
                     /gene="ftsZ"
                     /locus_tag="KB950_000399"
     CDS             complement(8607..9848)
                     /gene="ftsZ"
                     /locus_tag="KB950_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBB6856641.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9871..11196)
                     /gene="ftsA"
                     /locus_tag="KB950_000400"
     CDS             complement(9871..11196)
                     /gene="ftsA"
                     /locus_tag="KB950_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBB6856642.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11352..12572)
                     /locus_tag="KB950_000401"
     CDS             complement(11352..12572)
                     /locus_tag="KB950_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBB6856643.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12587..13675)
                     /gene="murG"
                     /locus_tag="KB950_000402"
     CDS             complement(12587..13675)
                     /gene="murG"
                     /locus_tag="KB950_000402"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBB6856644.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13701..15062)
                     /locus_tag="KB950_000403"
     CDS             complement(13701..15062)
                     /locus_tag="KB950_000403"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBB6856645.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15076..16038)
                     /locus_tag="KB950_000404"
     CDS             complement(15076..16038)
                     /locus_tag="KB950_000404"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBB6856646.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16064..18256)
                     /locus_tag="KB950_000405"
     CDS             complement(16064..18256)
                     /locus_tag="KB950_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBB6856647.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18257..18658)
                     /gene="ftsL"
                     /locus_tag="KB950_000406"
     CDS             complement(18257..18658)
                     /gene="ftsL"
                     /locus_tag="KB950_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBB6856648.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18663..19622)
                     /gene="rsmH"
                     /locus_tag="KB950_000407"
     CDS             complement(18663..19622)
                     /gene="rsmH"
                     /locus_tag="KB950_000407"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBB6856649.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19643..20074)
                     /gene="mraZ"
                     /locus_tag="KB950_000408"
     CDS             complement(19643..20074)
                     /gene="mraZ"
                     /locus_tag="KB950_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBB6856650.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20244..20612)
                     /locus_tag="KB950_000409"
     CDS             complement(20244..20612)
                     /locus_tag="KB950_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBB6856651.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20783..21739
                     /locus_tag="KB950_000410"
     CDS             20783..21739
                     /locus_tag="KB950_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6856652.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21812..21928
                     /locus_tag="KB950_000411"
     CDS             21812..21928
                     /locus_tag="KB950_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBB6856653.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21983..23665)
                     /gene="recN"
                     /locus_tag="KB950_000412"
     CDS             complement(21983..23665)
                     /gene="recN"
                     /locus_tag="KB950_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBB6856654.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23683..24132)
                     /locus_tag="KB950_000413"
     CDS             complement(23683..24132)
                     /locus_tag="KB950_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBB6856655.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24239..25057)
                     /locus_tag="KB950_000414"
     CDS             complement(24239..25057)
                     /locus_tag="KB950_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBB6856656.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25066..25956)
                     /locus_tag="KB950_000415"
     CDS             complement(25066..25956)
                     /locus_tag="KB950_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBB6856657.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25958..26182)
                     /locus_tag="KB950_000416"
     CDS             complement(25958..26182)
                     /locus_tag="KB950_000416"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBB6856658.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26187..27524)
                     /locus_tag="KB950_000417"
     CDS             complement(26187..27524)
                     /locus_tag="KB950_000417"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBB6856659.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27525..28379)
                     /locus_tag="KB950_000418"
     CDS             complement(27525..28379)
                     /locus_tag="KB950_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBB6856660.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28476..28925)
                     /gene="nusB"
                     /locus_tag="KB950_000419"
     CDS             complement(28476..28925)
                     /gene="nusB"
                     /locus_tag="KB950_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBB6856661.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28918..29343)
                     /locus_tag="KB950_000420"
     CDS             complement(28918..29343)
                     /locus_tag="KB950_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6856662.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29589..30653)
                     /locus_tag="KB950_000421"
     CDS             complement(29589..30653)
                     /locus_tag="KB950_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBB6856663.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30825..31070)
                     /locus_tag="KB950_000422"
     CDS             complement(30825..31070)
                     /locus_tag="KB950_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6856664.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31209..31583)
                     /locus_tag="KB950_000423"
     CDS             complement(31209..31583)
                     /locus_tag="KB950_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBB6856665.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31666..32580)
                     /locus_tag="KB950_000424"
     CDS             complement(31666..32580)
                     /locus_tag="KB950_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBB6856666.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32601..33404)
                     /locus_tag="KB950_000425"
     CDS             complement(32601..33404)
                     /locus_tag="KB950_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBB6856667.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33437..34426)
                     /gene="manX"
                     /locus_tag="KB950_000426"
     CDS             complement(33437..34426)
                     /gene="manX"
                     /locus_tag="KB950_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBB6856668.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34529..35020)
                     /locus_tag="KB950_000427"
     CDS             complement(34529..35020)
                     /locus_tag="KB950_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6856669.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35211..38018)
                     /locus_tag="KB950_000428"
     CDS             complement(35211..38018)
                     /locus_tag="KB950_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6856670.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38452..38745)
                     /gene="rpmA"
                     /locus_tag="KB950_000429"
     CDS             complement(38452..38745)
                     /gene="rpmA"
                     /locus_tag="KB950_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBB6856671.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38758..39102)
                     /locus_tag="KB950_000430"
     CDS             complement(38758..39102)
                     /locus_tag="KB950_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBB6856672.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39129..39437)
                     /gene="rplU"
                     /locus_tag="KB950_000431"
     CDS             complement(39129..39437)
                     /gene="rplU"
                     /locus_tag="KB950_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBB6856673.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39457..39533)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39719..40360
                     /locus_tag="KB950_000432"
     CDS             39719..40360
                     /locus_tag="KB950_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBB6856674.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40422..42053)
                     /locus_tag="KB950_000433"
     CDS             complement(40422..42053)
                     /locus_tag="KB950_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBB6856675.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42176..42535)
                     /locus_tag="KB950_000434"
     CDS             complement(42176..42535)
                     /locus_tag="KB950_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBB6856676.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(42694..42767)
                     /locus_tag="KB950_000435"
     tRNA            complement(42694..42767)
                     /locus_tag="KB950_000435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42731..42733),aa:Arg,seq:tct)
BASE COUNT        12084 a         9298 c         7253 g        14142 t
ORIGIN      
        1 tgagtcttct ctatcatctt tcgttttttg ttgtaaaaac gtctaaaaat cctttgtttt
       61 ttatttagaa aaatagtcaa ttaacgtttt ttttgttaca cttactaatg atggtttttt
      121 accatgaaag gatgatcgaa tatgacacca aatcgagaag attatttgaa aatcatttta
      181 gaactaggtg gagatacaac gaaagtcaac aataaacaaa tcgtatctag tctagatgtc
      241 tcagctgcat ctgtcagtga aatgatttct aaacttgtga aagaagagct tgtagaacat
      301 tccccttatc aaggtgtaca attgactgca agcggactgc aaaaagctag cagtctgatt
      361 cgcaaacatc gcttatggga agttttttta gttgaacatt tagactatag ctggaatgaa
      421 gtccatgatg atgctgaagt attggaacat gtgacttcgg aacatcttgc agatcattta
      481 gaaaactatt tgaatcatcc tgaacattgt cctcatggag gatcgattcc taaaaaagag
      541 gaagttgtcc atgaagaacg ccgacaaaca ttggagtcct atcctgtcgg tacaaaagtg
      601 cgtatcgcac gagtgcttga tgaaaaagaa ttgttggatt acttagtcac tattgacctg
      661 aatatcaacg aagaatatga gatcgttgac atcgcagctt atgaaggacc gatcactatc
      721 caaaatgata gaaagaaaat ccccgtcagc tttaaggcgg ccagcacgat ttttgtcgat
      781 aaaatataaa ggagtgtatc cacatgtcta atgaaaaaaa tgtattgttt acgatattcg
      841 gcggtacagg tgacctagcc cagcgtaaat tgtacccgtc tttgttccgc ctttacagaa
      901 aaggaaattt aggcgagcat tttgctgtaa tcggaaccgc tcgccgtccc tggagcgatg
      961 agcactaccg tgaagttgtc aaagaaacaa tccgtgcatt gaatccttca gatgaagaag
     1021 caactacctt tgcaagccat ttttactatc aatcccatga tgtaaatgat tcaacgcatt
     1081 atcagacatt aaaagaattg tctgaccgat tagatggaca ctatcaatta aacggtaacc
     1141 gtgtctatta tttggctatg gcacctcaat tttttggaac gatcgtttct catttgaaat
     1201 cacaaaatat tatgacaaaa aatggcttca atcgtttgat catagaaaaa ccgttcggtt
     1261 ctgattacca gtctgctttt gaattgaacc agcaaatcag agaagtattc ccagaacaag
     1321 atattttcag gattgatcat tatttaggaa aagaaatgat ccaaaacatc tcagctatcc
     1381 gttttgccaa taatattttt gaggctcaat ggaacaatcg ctatatcgac aatatccaaa
     1441 ttacttttgg cgaaaacctc ggtgtagaag atcgcggggg ctactacgat catagtggcg
     1501 cacttaaaga catggtgcaa aatcatattt tacaggttgt agcattactt gcaatggaac
     1561 cgccagtagc attttccgaa aaagaaatcc gtacagaaaa gatcaaagca ttgaaagcta
     1621 tccgcattta taacgaagaa gaagtgttag aaaacttcgt tcgcgggcaa tatgctcaag
     1681 gtgaattgga tggtgtacaa ttcaagggct atcgcgaaga agacaaagtg gatgctcaat
     1741 cagagactga aacattcgtc gcaggcaaat tcactatcga taacttccgt tggtcaggtg
     1801 ttcctttcta tgtacgtaca ggcaaacgtt tgactgaaaa agggactcgg atcaacatcg
     1861 tattcaaaca agttcctgtt aatgtattca aaacatctgt cgatgaacca tgtgacgata
     1921 cgacacttcc acctaatgtt ctaacgatct atattcagcc aactgaagga ttttctttat
     1981 ccttgaatgg gaaagaagta gggcaaggat ttgaaacgga accaatcaaa ttggaattta
     2041 gaaatagtgc agaaatggtt gaaaatagcc cagaagctta cgaaaaatta ctattagatg
     2101 cgttgaatgg tgatggaacg aacttctctc attgggaaga agtcgctcaa tcttggcata
     2161 tcgtggatgt cattcgaaaa gcttgggacc aaacacaacc ggattttccc aactacaaag
     2221 ctggatcaat gggacctaaa gcagctttcg aactacttga aaaagacgga ttcgaatgga
     2281 tctggcaacc agataactgg tatcgtgaac gtggaaaatt aaacgattga taacaaaagg
     2341 gggtgtgaca aagaacatgt cacaccccct tttccctttt acttttcttc gaatccttca
     2401 gctacagctt caggatagaa gtcttcaacg atttttgcac aacgaccgca tagatgagga
     2461 agtttttcat ctactcctac atctttacga acttggcgac agcgatcgca agtttccccg
     2521 tctgcttttt ccactgtaat agcaacatca tcaaatacca tagcgttatc tggcacttgt
     2581 tcatctgctg ccataacttc aaagtagtct ggtgaaacga tcaataattg tgcaatatct
     2641 gcatccaaag cttttaacat ttcacgaact tgttgttttg gataaacagt tagttttgct
     2701 tccaaagatt taccaatcaa tttgctgttt cttgcttctt ccaatgcttt taagacatta
     2761 tcacggaagt ccatgaaagc agtccaggta tccatcaatt cttcttggtt tgcaaactct
     2821 tcataaccag gaaactcgct taactgaacg tactcctctt cttctttcag gaatgaccag
     2881 atttcttctg ctgtatgcgg aatgatagga gtcaataatt ttgtcaaagc tacagctgtt
     2941 tgatagaaaa cagtttgcat gcaacgacgt tgataatcat cttctgcttc aatataaact
     3001 acatctttag caaaatccag gtagaatgac gatagatcaa ccgtcaagaa gttcataact
     3061 gtacggtaaa tatgcatgaa gttatatttg tcgtaccctt cttctcggat ttctttgatt
     3121 acttggttca atcgaacggt catatatttg tctacagaac gtaaatcatt gtacgaaaca
     3181 gtatcttttt taggatcgaa atcttctgta ttcgctaata agaaacgcat cgtatttctg
     3241 attttacgat atacttctgc cacttgactt aagatatcca tagaaacacg aacatctgct
     3301 tcataatcta cactagatac ccataagcga aggatatctg caccaaattg tttgatcact
     3361 ttttctggtg caatgatgtt tcctaaagat ttactcatct tacggccttc accatctaaa
     3421 gcaaatcctt gagaaataac tgatttgtat ggtgctacac cattgatagc cacactagtc
     3481 gtgatacttg agttgaacca gccacgatat tggtctgatc cttctaaata catatctgct
     3541 gggaatgata attctggtcg ctgacgtaaa acagcttcat gagaagaacc agaatcaaac
     3601 cagacatcca tgatatcttt ttcttttgta aattcgccat taggagaacc tgggtgggta
     3661 aatccttcag gaagaagttc tttcgcttct ttttcaaacc agataattga tccatgttca
     3721 gcaaataatt ttgccacatg ttcgattgtt tctggtgtga tgattgcttc tccattttca
     3781 gcatagaaaa tcggaagtgg tactccccat gcacgctgac gggaaattac ccaatcgccg
     3841 cggtcacgga tcatattata caaacgtgtt ttaccccatg ggataaccca ctctactttt
     3901 tctacttcat ccaaaatatt ctgacggaat ttatcgatag atgcaaacca ttgtggagtt
     3961 gcccgataga tcacaggctt tttcgtccgc caatcgtgag gataactatg agtaaagaaa
     4021 tctagcttca gcaaagctcc tttttctttt aatagatcag tgatcatagg atttgctttg
     4081 tcataaaaga taccttcaaa tccaggtgct tcttctgtaa acaatccttt tgagtctaca
     4141 ggtgacacta cagatagttt atatttgcta ccagcgatat agtcatcttc cccatgtcct
     4201 ggagcagtat ggactaatcc agtaccagcg tctaaagtaa catgatcgcc taaaatcaca
     4261 agtgattcac gttcatagaa tggatgactt gcagtcatat attccatttc ttgtccagca
     4321 aattctttta gaactgttac attttcccaa ccgatttctg attggactgt ttctagcaaa
     4381 tctttcgcta cgacaaactt acggccatcg gcttccacca atacatacgt aaattttgga
     4441 tttacagcaa taccttggtt tgctggcaat gtccatggtg ttgtcgtcca aatgacaaaa
     4501 gctgtatctt catcaagaat atctttgccg tctttgacat tgaaagctac aaaaatagat
     4561 ggagatttta catctttata ttcaatttca gcttccgcta aagaagactc acttgacgga
     4621 gaccagtaaa ttggtttcaa tcctttgtaa atatagcctt tttcagccat tttaccaaat
     4681 acgcgtactt gtgcagcttc ataagaagga tctaatgtaa cataaggatg ttcccaatct
     4741 cctgcaactc ctaggcgttt gaaatctgct cgttgcttat ctacttggga aagtgcatat
     4801 tcacgacatt tttccaagta ttctgccatg gacatttctt tacgtttcac gcctttatta
     4861 gccaatactt gttcaatcgg caatccatgt gtatcccatc ctggaacata tggtgcacga
     4921 tagccagaca ttgattttga acgaatgata atgtctttac tgattttatt caatgaatgt
     4981 cctaaatgga tattaccatt ggcatatgga ggtccatcat gaagaacaaa gctaggtttt
     5041 ccttcgttca actcttgtct tttttggtag attcgatttt cttcccattc tttttgccac
     5101 tcgccttctc ggtttggcaa attaccacgc atagggaatt ttgtttttcc taattgcaag
     5161 gtttctttca ttttcattca accatacact cctttatttg ttttccggac acaaaaaagc
     5221 atgttcagcc gcaagggacg aacatgccgt ggtaccaccc aaatttagtt gcatctatct
     5281 agtgcaaact ctctgtgacc aataacgagg tcagccgctg cttcttactc taatttcgga
     5341 aaagcaggtc tcaagtagag gatctttcac catgcagtgt cttaccgaac ttccactctc
     5401 atcggctcgc tttaagaata acaaagtgct tttctgttct actcgttttc attttcacca
     5461 ggcacgatcg agatatcaat agcctgtgta tcaaatgccg tcataggtgc ttctatgact
     5521 cctgtgtctg agtttacatc agtatcattt tcattgtcaa gctgttttgc taaaacttct
     5581 ttgatcactt catggctgtc agtcacgtag ctggagaatg gttgtaaaat ttcatcccat
     5641 tccggacttt tcacttgttc taattgagac tcaagcatca agctgatacg ttgatggaat
     5701 actcgtgttt ttttcttaag atcttcagtt tctgtcgcaa gctgtcttgc tttttctgtt
     5761 gcatctgtca agatttttct tgctttttct tctgcatcag ttgttaattg atgtgcacgt
     5821 ttttcagcgt tcgcaactaa ctcatctgct ttgttttgtg cagaagtaac gatcacttcg
     5881 gattccttgc ttgcacttgt ttttacttta tcagccgtat cttgagctac gatgattgat
     5941 tggttcaaag catcttttaa ttcattgaag tactcaagtt tttcttcaga atgtttgact
     6001 gctttttcta attctcgatt tctttgagtc aattcctcat agtcacgaac aactaaatct
     6061 aagaaatcat ctacttcgtc ttgattatat ccccgcattt ttgtcgggaa tgtcttattt
     6121 tggatatcta atggagttaa agccatgagt aaaccacctt tttattttat ttacgtaata
     6181 caccgaacag taccctgtac ttttcttttt tcgtcttccc ctcaagttct tggatctgga
     6241 ctctgccata acctctgact gaaataatat ctaatagttc aagtacaaaa tcaggtcttg
     6301 tattttctgt ccagttcact tttactttac cagcttcgat cagctgcttg gaccgctgtc
     6361 tggaaatatt ataaatctca gagatcacac tgtctagtcg catggaacta actgttcctc
     6421 tttcttgtgt ccagctatct ttcggcataa tcatttctgt gtatttgatt ggttccaagc
     6481 gtacagcaac ctttccaatc tttgttactt gtgcagcaac aaaatgatcc acctcatttg
     6541 caataaatac ttgccagcga tcaccgtccg agatgatatc gccaaagtaa gagcgtttga
     6601 tccccgttcc cactaaagtt ccaaggattt tcccatgact taggctggca aatttgctag
     6661 gataatggat ttcatacaat gctgtttcaa aatcttcctc tacaggttcg taataatcgg
     6721 ggaaaatcaa acatcttttt ctttctgcct gttcatatcc gccataaaac atgaaacgca
     6781 aatcactgct ttgtcgaatc agtgtttcta aaatatagga ttgtcttggg tcaagaaatt
     6841 cggtcagata gggtgcatat tgtccttgaa cctgttcaat ccaatcgcct accgaatcga
     6901 taaatgtacg ttcatctgcc cgaaagtgct ggtatacatt tgcatccaaa attttcccct
     6961 cctatctatt ctgagaataa agattttgtt gcttcaatca ataaactaaa tggacagtta
     7021 tccatataac gatctgtgct aaaccttgta cggctaagtt caagatcagg attgccccta
     7081 aaattgtgaa atccaagggt ccaatcctca aaggcagacg atcgaacaaa ctgatatacg
     7141 gttcgcagat cttagctaat aatcgtccta gccctgattg atacccgcct ggaaaccaag
     7201 agagcaatgc atataccatc aagataatgc tatagaaata ggctgcccgt tgaagtaaat
     7261 gaagtaatag tgcaatagta taaatcgtag ttcctcctct tacagatcaa acaaattcgc
     7321 atctgcaaaa gattgagcgg ttgaactgtc aatctccatc ccagtcggtg tacaaagaaa
     7381 aatctcatct ccaacacgtt tgatatctcc atctagtgca taaactgttc ctgttagaaa
     7441 atcaacgatt cttctcgctt gtttttcttc tactaaatga aaattcacta aggttgcttc
     7501 tccagcaatg attcgtttag caatcgccat agcttcagaa taagctcttg gttccattac
     7561 agtgattttt ccagcaagat ttgctttagg ttgtgtagag gttcttgaag ctgttctttc
     7621 tttcgctaca gtttttgtag gtgattgatg tagttcaatt acttttttct tttcagccgg
     7681 ctgaatactt tcatgcgtta caggacgtga agtcgccgtt ttttgttttt catgagtatt
     7741 cgcagcagat acagaatgag ttgattgatt ctgtgttgta ttttttttag ttggctgtac
     7801 agcctttttt gttgtttcag gataatcttg gtattcgtac tcttcctcat cagctaatcc
     7861 gaaaaaatta gcaattgctc ctctgtttaa aagcggcatt tttgttcctc ctttattcgt
     7921 ctttgaatag cgctgttcct acacggataa atgttgcacc ttcttcaatc gcaatttgaa
     7981 agtcttgact catccccata cttaattcac tacatggtgc atgggcataa ccttttgctt
     8041 gaacaagatc acgcaattct ttcagcgtac caaacacttt atggatttgc tcatcagaag
     8101 cttctttagg cgccatagtc atcaacccaa caaccttgat ttttgtgcag gcagctaaag
     8161 attccacaaa cggtaaaagc tcatctggtg aaattccatg ttttgattcc tcaccagaaa
     8221 catttacttc aataaaacag gataattctt tctctaaccg cttctggatt tcttctgcta
     8281 atcttaaact gtccaacgca tgaaagtaat caatttcatt tacaatcaat tttacctttc
     8341 tgcgctgtaa attaccaatt aaatgccact ttatttcagg aaaatcaaat aaatcttgtt
     8401 tcttttctaa taatttatct actcggtttt ctgcacaatt cataacgcca gctctaacca
     8461 attcttcagc ttgagcggat tcaacagatt ttgttacagc tactaatgta acttctttcg
     8521 gatcacgatg aacaagagca cacgaatcct gaatttcttg cttgattttc tgcaagttgt
     8581 cagcaatcat gtgctcttcc tctcctttat tttttacgac ggaaaaacgg tggcgtattt
     8641 aattcatcat cgccatgaga ttttgtttct tcgcggttga atgtatcaaa ttcttttttt
     8701 tcgatattgt caaaattgga atcatcaact ctaggacgaa cattttgttc acgtctgata
     8761 tcccaatcgc caaaactgtt ttcttcttct gaagaagttg gtttagcttg atccatatct
     8821 aatacagtct tttgagcagg tgattgcatt tgtgcttgtc tagctggtcg gctagacttg
     8881 cgttcctttt ttgattcatc gatacctgtt gcgatgaccg tcacacggat ttcatcgccc
     8941 atttcttcat tgatagatgt acctagaatg atatttacat cgccagttgc tgcatttgct
     9001 acgatatctg aagcatcttg tgcttcgaac aatgtcatat ccaaaccgcc tgtgatgtta
     9061 agaagtactt gttcagctcc atcaatagat gtttcaagca atggagagga gattgctttt
     9121 ttcgttgctt ctactacgcg atcttctcca ctagccacac cgatccccat caatgctgta
     9181 ccttggtttt ccatcactgt tttcacgtca gcaaagtcaa ggttaacgta acctggcgct
     9241 gtgatcaaat cagagatacc ttgtacacct tgtcgtaaaa cattatctgc ttcacggaaa
     9301 gcttcaagca tcggtgtctt tttatctaca acttctaata aacggttatt tgagataatt
     9361 aataatgtat ccacattttc tttcaaacga gcgatacctt ctgcagcaaa acggccacgt
     9421 ttcggtcctt caaaagtgaa tggacgtgtc acgacaccaa cagtcaatgc gcccaattct
     9481 ttcgcgatac ctgcaacgat tggtgcagca cctgtaccgg ttcctccacc cattcctgca
     9541 gtgatgaaaa tcatatctgc accgtctaaa gcttcgcgta aggattgttc actttcttct
     9601 gccgcttttt gtccaacttc tggctgagaa ccagcaccta aaccacgagt atatttgggg
     9661 cctaattgga taactgtttc agcttttgaa ttttttaatg cttgcacgtc tgtgttggct
     9721 gtaatgaatt caacaccttt aacgttttct tcaatcattc ggtttacagc atttccaccg
     9781 ccaccaccga ctccgatgac tttgattact gcgccgtcat tgatattatt atcaattgag
     9841 aattccatat cttattcctc ccaggatgaa ttaatcaaaa atatttgtaa agaaaccctt
     9901 gattttattt tttacatttt ctccggactc ttttggttcc ggctcatcgt agattgtttc
     9961 ttcaggtgct tcatagctct catatgtgtt tgttgtttct tgttcaacta ccatttgacg
    10021 agctgctgag gtttcacccg taacagcaat ttttgccaat tgatacactt cactcaagtt
    10081 tgctgaataa tccacgatac ttattacatt tgtaaatacc gggtttctca gccccatgtg
    10141 atttggaaca tacaatttta catttacgcc gaagatttct tgtgccaagt ccacaacacc
    10201 aggaagacta gcagctccgc ctgtcaatac gacgccacct ggcaactcta atgcttcgat
    10261 ttggtccaat gcatcttttg ctttgttgaa aatctgttcc attcgagctg aaataatttc
    10321 tgacaagtag cgttcatcga ctttcactgg ttctgactga ccaatcacat ctactggaaa
    10381 ttcttcatct ggtgatgtcc gttctgggta tgcatccccg tagtttattt tcaacgcttc
    10441 tgcattgtta aatgacgtat ttaatacaat agagatatct ttagtgacaa attcgccacc
    10501 ttcttggtca agattggtaa acttcaattg cttatcatgc ataacagccg ttgttgtttg
    10561 gccgccaccc atatcaatga cgatcgtacc aaaatctttt tctccgtcag aaagaattga
    10621 ttcagtcagc gctaaaggag taatcaccat ttcgttgacg attaatccgg cattttccac
    10681 gcatttacgt atgttatgga tgatcgtttt aggtcctgta aacaacagac cgtacatttc
    10741 taatcttacg ccgatcattc cacgaggatc tttgattccc tcgaacccat cgacagtaaa
    10801 atcttgtggc aatattgaca cgatttgacg ttcaggaggt attgaacgaa caagggcagc
    10861 tgaagccaca tttctgacat cttcatcggt gatttccttt gaatctccgt taactgcaat
    10921 cattccctgg caattttcta cttcaagcat gtttgctggc aaaccgacac ttacgccttt
    10981 gatctggata cctgcttttt cttctgcttg tcgtactgct cgttgtatcg cttggaccgt
    11041 tttatcgata tcaacgataa tgcctcggtt aatcccttct gattttgcgt ttcctactcc
    11101 aataatattc atttggctgt cgatatattc agccacgaca actttaacag atgtcgttcc
    11161 aatatcaagg cctacataca ttcctgtttt tgccatgaat gggattccct cctacttact
    11221 attccatata caagcgaaca actagcgtcc gttttaaaat atacttatta atagatatat
    11281 tcactgcttc caattctacc ataaattact ggtttttaga aagtacattc ttacttttta
    11341 aagttgtttt atcaattttc atttaaactt gacggagttg tacttggatt atcttctatt
    11401 tgacttgttt cacttgaatc atttgaatca gatgatccgt ttgtgctaga cgatccaggt
    11461 gtttgtgctt tttgcgtttc tgcctcttca cttgattcgt ttgtttgatc cgcattttct
    11521 tcactttcag ttgtttcttc cgattggccc gattcttccg tttcttgagt gctaccgtaa
    11581 ggatgcgaga agataccaac ttccatgtcg atcacacctt tttcatccat atctttggct
    11641 acttgcgaat aatatttcat ttggttcgca agattattga tatttacaat aacttgattg
    11701 ccgtcattca tattcaattg gatcagattt ttattgcttg ctcttggcgt gtacttgatt
    11761 tcagaaattg ctttttgtaa ttctgatgat aactgattgt attgctttgt taattctttg
    11821 atttttgcag agtctttgaa ctcttccaag atagggagat tttttgtagg atttgctact
    11881 ttttcatcta atactgttcc attttcaatc actgggtagt attgattgtt ttttgcgatc
    11941 aaagcaatct ctttatattc tgttacatcc aaatcgaatt cattcatccc tttgaaatgt
    12001 atatttgctg tttcgattct tggctgtgct tttttaaggc gatcgatata agtagatcga
    12061 tgaaaatact gtccccaaac attttctcct aatgacaatt ttgtatcaga gatgatttct
    12121 tggctgttga ctgtcttatt gcctgatact gcaactgctt gtaatttgct ataaggagaa
    12181 acataataga tcaaaaaaat cagcggtata cccaatacag tgatgatcaa agctaaccga
    12241 cgaaatagta ctttgtgccg ttgacttttt aaattgggca gccggttcaa aaaagaaccg
    12301 ttatatggtc caccgttttt cttttgcttg atctcttcaa aaaattcgat ttttttctcg
    12361 gactggcttt cttcttgctg ttcctcttga accgtttttt cttcttcaga atctgaaagg
    12421 ggtgtatcta atgttatgtc ttcttttttt ggctcttggt ttttcatctc ttgttctgcc
    12481 agatattttc ggttagcctt ttgccaagga gtcaaattgt tttcatcaga agacggatga
    12541 gacaattttt ttggttcttt tttcttactc actaagacca ccgcctttat tttttgatta
    12601 tttgttttac taagtcataa agacgttttg aagcatcagg gattccttgt tctttagaag
    12661 ctcggcacat ttgcttttgc aattcttcat cacccatgat ttcgttgact gtctgactta
    12721 gtgattcacc tgttagttca ttatctgcaa tcatttttgc tgctcctgcg tgaacaagac
    12781 tcattgcatt tttagtctga tgatcattcg taacatatgg gctgggaacc aacactgccg
    12841 gtaaccccag agctgtgaat tctgcgatcg aagttgcacc agcacgcccc accagcagat
    12901 cactggaagc catgacctct gccatatttt tgatataagg ctggacacta atattttgta
    12961 tttcaggatt tattttattt aggatctctt cataatatct atctcctgaa gcatacaaaa
    13021 cttggtaaga agaaggaata tattcactta aaaaaactga tactgcttga ttgattttaa
    13081 gtgctccttg actaccgcca aaaatcaaga ctgttttctt ttctggatcc aaatcaaatg
    13141 ttttcaaaat atctgatttt ttagtatctg ctacttcttg tgctcgagga ttacctacaa
    13201 gaactgcttt gttttcagga aaaaaggaag ccgcatcttt aaatgaaaga gcgatccgat
    13261 ccacatatcg gcttaaaaat ttattggtaa cacctggtac actgttttgt tcatggataa
    13321 ctgttggaat acccattttg gctgcagcgt acactaccgc tccggaaaca tatcctccag
    13381 taccgataac gatatctggt tgaaaatcac tgaggatttt ttttgcttga cggatactct
    13441 taagaaacaa tcggaccgtc ttgatgttat gcatggaaat ttttcgttta aatccttgaa
    13501 tctccaaggt atggaaaggc attcctgtat cagggacgat tttgttttct agacctcgtt
    13561 ttgctcccac atacatgaat tcagcattcg gttcttgagt ctttacataa ttgacaaagg
    13621 caagagctgg ataaatatgt ccccctgttc ctccgcctgt aaccaaaatt ttcatttttt
    13681 atcgctcgct tctacttttt ctattttaat cggttcacag cttccatgaa tctgtttcct
    13741 ctagtttcaa aatttgggta ttgatcccag ctggcattcg ctggtgataa taaaactaca
    13801 tcaccatctg tactgttttc taatgctaaa ggaactgctt gttctacaga atcggctgtt
    13861 actattgtct cgatgcctgc tttttttcca gcatccatca acttttcttt tgtttggcca
    13921 aatacgacca ttgctttgat tccttttaat gaaggaatta attcatcaaa tgtatttcct
    13981 ctgtctaatc cgccagccaa taacacgacc ttagacgttt caaatccact tagagccatt
    14041 ttagtcgcta aaatattggt tgcttttgaa tcattataaa attttctgcc ttggatttct
    14101 cctacatatt gcgtacgatg tggtacgcca tggaaaaaat gcaacgtttc tcgtatagct
    14161 tcatttgata taccatacaa ttttgctaca gaaatagcag ctagtgcatt ttctacatta
    14221 tgcttacctg gtacgccaag ttcagatatt tccattattt tttctttttt atagtatatt
    14281 gagtaatcgt cagcatacac accgtcttcc aacacttctt ttgtcgagaa aggcagtacg
    14341 cgtgcttttg ttctttgcgc taattcttgt agttctgact gattccagtt aaggatcaga
    14401 taatcttttt ctgtcatgtt tttttgaagg ttccatttag ctttgacata ttcttttctt
    14461 gtaccatgat aatcaatatg tgcttcatag atattggtaa tcaccgctat atgagggcgg
    14521 aaatctgtga ttcccatgag ttggaagcta gacaattcca tcacaatttt atcttctgat
    14581 tttgcttctt gcgctacacc acttgctgga tacccaatat tacccgctaa acgtgcaatt
    14641 ccttgatctg ctcctgcatt cagtagtagt cctgtcatcg ttgttgtcgt tgtttttccg
    14701 ttcgttcctg taatcccaat aattgggcat tccgctactt cgtaagctag ctcaacttcc
    14761 gtaattaccg gaatacccat ttcttgggct tttgctacta aaggatgact gtagggaatc
    14821 cctggatttt tgaccatcaa tgaaaattct tcatccaaca attcgattgg atgactacct
    14881 gttattactt ttatccctaa agaaagcagc tcttgtgctt ccggattttc gtcaaaaggt
    14941 tttccatcat taacggtgac aagtgcgccc aattcatgta ataatttggc cgcacttact
    15001 ccgctttttg cgagtcctaa gacaagcact ttcttatttt catatgttga tatttttttc
    15061 atttcccgtc atctcctacc aaacgatcca taatgtgatc aacgaagtca caatgctgat
    15121 caaccaaaaa actgtatcaa tcttccactc cgaccatccg cacatttcaa agtgatgatg
    15181 aattggagac attttaaaaa ttcgttttcc tgtaagctta aaggaagtaa cttgaagcat
    15241 cacacttgct gtttccataa catagatcaa accaatcaaa agcaatgtcc attcttggtt
    15301 tagcattata gaaattgccg ctaaaagtcc gcccaacgct aatgaaccga catctcccat
    15361 aaaaatcttg gcaggttttc tgttgtagac gaagaaaccc agtaaacctc ctaaaacact
    15421 taagcaaatg attaatacat catactgatg ctgatgccaa gcaattagac cgtaagctgc
    15481 aaaagagata cttcctaagc cagcaactaa tccatcgatg ccatctgtca gatttactgc
    15541 atttgaaaat ccaacaagcc agaagagagc gaaaatacca taaatgattc ctaaaggtag
    15601 atctagtccg aagaaattca atacacctgg ataaccttca gaacggtaaa caagataaaa
    15661 gatgattccc cctagaatct gtccaatcaa tttctgcttc gagttcagtc ccatgtttct
    15721 tttcttgaat atttttataa aatcatcaag aaagccgatt actccgtata acgctaatac
    15781 aaaaagcaag atgaaaagag taggagttag ctgtttttgc caagctccaa cccaaattcc
    15841 tgtcaaaata gagccgatca gaaacactaa gccgcccatt gtaggagttc cggctttgct
    15901 attgtgccac tttggtccct cctcacgaat cgcctgacct tgctttttca tttgaaaata
    15961 accaataaat agcggcattg cggcaattgt cattgcacaa ctgctgacaa tgggaatcaa
    16021 tgcttgtgtc cattccatgc tcttttctct cctatttctc ttcttattct tctaaagtaa
    16081 aactaagttt gtcttcagtg attttttcat atggagcaag ttcttgtttg acacaataac
    16141 catctccatc aaaactaact tcaataccga ggattttccc cagcttcatg atatctgctt
    16201 ttgaccatcc tgttacgtca ggcatcagct tatcaccacc agtatatagg atcagttttt
    16261 ctccagagat caactgatca ccgttggcag tagattgttt ttgtactttt ttaccattac
    16321 cgatcactac aggttgcagt ccactttttt gagcatcagc tgctgcaacg tccgcttcaa
    16381 gattacgata atcggctacg gatacttttt ctgtttttgt ttctgtatct gaatcttctt
    16441 cagtttcttt aaaatccatt gcccgtttca tcaatggatt tgcgatttta gccaatgcca
    16501 ttcgatcata tgtcttaggg tgtttcattg ttagatacaa gacatagtct ggatcttcag
    16561 aaggaaccat ctccacaatc gaatacaggt aggctgtatc tcctgtttga tatccccctg
    16621 tatcagaagc gatttgggct gttccgtttt ttgccgatac attgtatcca ggtacactat
    16681 atacaccata agcactaccg tagttttcgc tttcgacaac atctctcata tattcacgga
    16741 ctttttctgt tgtttctttt gaaaaaggtt gccctagaac ttctgtttga gttgttcgct
    16801 cttctccagt ttggggatcc accactttgc tgatatatcg aggttgaatc attgtaccgt
    16861 tattcgcaat agaagtgaaa gctttcatca tttggaaatt cgtcacacct accgcttgtc
    16921 cataagcact cattgctcgg tcgacaatat ttaatgtagg taaagcaccg tttacttcat
    16981 catctaatcc agaatgagta ctttgtccaa atcccagttt ttgtaaatag ttataccatc
    17041 gtccgcctaa tcgctgttcc agtatgacca tcccgacgtt acttgaccat gaaagagctt
    17101 gacgcatggt cagcacacct ttttcaccaa agtcatggtc attgatcgta gcatcttcta
    17161 cttggatttt tccagattgg aaggtttcat tctcgttgaa ttctccttct tcgatagcag
    17221 ctgcagtagt gaaaactttc atggttgatc ctggttcata actatcttgt accaagaaat
    17281 tacgccagat agcatctttt ccagtcaatc cttccattgt ttcaggattg aaagtaggtc
    17341 gttgtcccat tgccaagatt tctccggttt ttgctttcat caggactgct gtcaattctt
    17401 ctggctgata ttcttcattt acttgatcca tcagtgtttc taaataactt tgtagtctgc
    17461 tgtctaaagt cgtgtagata tcttgtccgt cgacagcttt ttcttcttct gccactgtac
    17521 ctggcagagg attttgaaaa ttatcttttt gataaataat cttaccatct ttaccgctca
    17581 agatatcatt gtaagcagac tccaaaccta gtttaccaac taagccattc tcatctttgt
    17641 ctggaactgc gtatccaata aaatgagaag aaaagacgcc atttggatac atccgagcct
    17701 gatggtcgac aaaataaaga ccagcgacac cttctttttt catgtccgct tcgatgtttt
    17761 gcttggtttc ttgggagata tttttcccat agctaccaaa ttcaacttga tacagctctt
    17821 tttttgcgcc gtcctctaaa atattcagtg catctttttt gtctatagaa agattcttat
    17881 gcaggatttc tgctatttta tcaaaattct tttcttcaac atacagtttt ttatccccag
    17941 aggtatatgt ttttgagagt atcgctttaa ttgaataaga ggaggcatct tctgcgagcg
    18001 ccacaccgtt acgatcataa atcgttcctc tttttgcttt tactacttca cttccttggt
    18061 acaactgttt tgttttttca gctaatgagg taccagcaac atgacctcca ataacgatat
    18121 acgagaaacg gacggcaaat aaaaagaaca atccaatact cgtagcaaat aaaatgatac
    18181 ctactttttt gcgattgttc atcggattta gatttttctt ttccataaac cgccgaattt
    18241 tatttttaag actcatttac ttcactttcc ttaaattgtc gtcattaata gacaatcctt
    18301 tttcttctgc gatttgttta atccgttcag tacgagaaag ttcgtttttt tcttgttcta
    18361 aactcgtttt ttcttgcttt ttatcattga tttctgtttg tattgacgtg atttcttttt
    18421 cagctccgct gatatttgtg cgcagcataa ctgttaaaac acataaagca acaatagcaa
    18481 tcgccacgct tgccacagca attttttcca aagcagaaat ctgctttaat cgttttgccg
    18541 gtgattgtgg aagaatgatt tcttgattcg aagaagaatc aggcatttgt tgttccggct
    18601 gatccaccaa ttcaggtaaa tcatattcgt aatcttgcat tttctttaat tctgccatca
    18661 ttctactcct ctttcattct tattttttct gcaatacgta actttgcact gcgtgatcga
    18721 ttgttttctg ttagctcttc ctctgaagga aggattggtt tacgagtaat gactttcagt
    18781 tctggttgga attcgtcagg tatgacagga agtcccggtg gtagatcttg taccgtacta
    18841 tattctttaa acatactttt gacaattctg tcttccaatg aatggaatgt gattacgctg
    18901 attcttccat tgactttcaa taaccggatt gcctgttcta aagaggcctc ttcggcaccg
    18961 agttcgtcat ttactgcaat ccgcacagct tgaaaaattc tttttgcagg atgcccgcct
    19021 tttcttcttg cgggagctgg gatgacttgt ttaatgattt cgacaagctc accagttgtt
    19081 tggattggtt ttgtttttcg tactcgttcg atttcacgag caatttgttt ggaaaatttc
    19141 tcctcgccat agcggaagaa aatctttact aattcatggt aggaatattc attaattacg
    19201 tgataagcgg ataaaggagc tgactgatcc atgcgcatat ccaacggtgc atcttgatga
    19261 taactaaatc cacgttctgc ttcatctagc tgaggagagg aaacccctag atcatacaaa
    19321 atgccgtcta ccttatctac atgctcactc aagcgttctt ctaattctct gaagttcgct
    19381 ttgataaatg tgaccatacc ttgttcgaca taagaagcta aacgcagctt ggcgtgatcg
    19441 attgcttttt gatcttggtc aaacgcatac agatgccctt tagaacttag ttggcttaat
    19501 aaatattcac tatgtccggc accgccgagt gtacagtcta cataaacgcc atccggtttg
    19561 atgttcaatc catctaccgt ttctttcaac atgactgtat agtgttgaaa tgtttcagtc
    19621 attagtcagc ctcttcctat tcttatagtc cgaaatcaat catactttca gcaatttcgt
    19681 caaagttctc ttctgctgct gcagaaaaat cttgccatcg tgtctcatcc caaatttcaa
    19741 ttcggttaga tacaccgatg actacgcaac cttttgtcaa agctgcataa tttcttaacg
    19801 tgctgggtat attgattcgt ccttgtttat caagttcgca ttcagtggca gccgaataga
    19861 agaatcgcac aaaagtacgt gcgtcttttt tagccaacgg catttcgttc agtttagctt
    19921 ccagattttc ccattcgctc atcggatagc cgaaaagaca gccatccaat ccgcgtgtca
    19981 gaacaaactt ttcgccaagt tcttcacgaa gttttgaggg cacgattaaa cgtccttttg
    20041 catcaatatt atgttggaat tcacccatca acattgaaag ccccccattc ctccactaac
    20101 taaaattagt ctaccacaat cccccacttt ctaccacctt ttctgggaaa tccgggaaca
    20161 acttttttta ttcataaaca tttttttaaa aatcaagctg caaaaagcct tttaaaagac
    20221 tttttacagc ttttctattt ttttcatagg taagaaagta tgttcacaat gatcaagaca
    20281 gcgtacaaga cgattgtcaa cagaaaaacc aaacgccaag tcatcttgaa ataacgcgga
    20341 taaataattt cttcataata atatgcttgg aaaacagcaa tacaaattcc cattagcaaa
    20401 acagaaataa agaaaaacgg taagatagaa attgtccctg tatttctaga caatgcatgc
    20461 ataccgcccc acaaaaaagg aatcgccaaa tcaggggctt ttattttaaa acgacgattt
    20521 aacgaaaata attttacaag aaattggcaa ccaaacaaaa cgattaccgg gtaaatatac
    20581 caaaacaaga taagtggtgt gaatgtgccc atgaacgtaa actccttttt tatcgttgct
    20641 tcaagtatac tgtatatact ttacacaact gtttcatttt atttaaattt atgcagttat
    20701 attctatatc ttacaaacac attccatgta ttccaattcc ctatccgtta taataaaaga
    20761 aacaggcaac aggaggaaaa tcatgatcaa atattttaca ttagataatg agaaactgcg
    20821 taataccgga aaggaaactg accaaacgat ctggtattgt gtagaacgtc ctaatcaaga
    20881 agagattcat cagctgacac atcaatttga aattccgtca gattacatca cttccatatt
    20941 ggacgatgca gaaaattccc gtacagaaga attcaatcaa tcaaagttga caaagccagc
    21001 gttattattg ttacaatatc cttttgctaa aacaagtcct agcggttatt ttcaagtgga
    21061 tacatacccg ctctctatta ttttgacgcc caaaaagaag cttatcacaa ttacgaatca
    21121 tgaaccactg tttttaaaga aagtctttag ccagaccttt ccaaaaagtg atatttcgcc
    21181 taatctcaat atcttctttc aattcatttg gcaggtcaca ctctcttaca acagttattt
    21241 agctacgctt caatctcaat gcgatacttt ggaagccaaa cttcaagtat ccactgaaaa
    21301 caaacaactt tatcaaatca tggatatcca aaaaagcctc gtttatttca gagaagcgac
    21361 ttctgctaat ttagatgcat tgaaagaact agcgcaaaat aaacttttcc aagaaaatca
    21421 tgctttgaaa aatcatttac gtgatgtatt ggtggaaaca gagcaggcaa tgactacttc
    21481 tcgcattcaa ttaaaactag tagaacaaat gaatcagact ttttcagcaa tcgtatcgaa
    21541 taatctgaat aatattatga aaattttaac ttctttaacg attattttaa ctataccgac
    21601 aatcgtcggc agtatctatg gaatgaacat taagttacct attgctgaaa gggatgatgc
    21661 ctttatatgg ctagttctat taacattatt tatttgtatc gtaactacct attatttaaa
    21721 aaaagggaag tttttataaa aaagccctct tgtggaaatg gaaaaaagat tgtacacttg
    21781 aatcaactaa ttaatgacga ggttgaaaac aatgcgtgaa aaaaaacaaa caagtacttt
    21841 tgctaaagta acaaaatttg tcatttggac aatgctgatt ttaacgattg gatctgttgt
    21901 attgactgct gtaatgagtg tcatgtaaaa ataaaatata gctatcagct gctgaaaagg
    21961 acctgaacca ctgcgaggtg gctcaggtcc tttttgcgag tttcaatagt tcttttgcat
    22021 gttcgagtgt gagttttgta atctcacttc cagccagcat acgtgcaatt tcttcttctc
    22081 tttcttttgt tttcaactct gaaacagaag tttgcgtacg tccatcaatc actgctttca
    22141 caatataata ttgatagtcg gcgactgcag caacttgagg cagatgagtg atgcacaaaa
    22201 cttgagagta tttcgatatt tgagaaattt tatctgcgat ggcctgtgca actcttccgc
    22261 tcacacctgt atccacttca tcaaaaataa tacttgtcac accttgctca gaagaaaaaa
    22321 tagttttcaa agcaagcaac atccgagaca gttcgccacc agaagcgact ttcaccaaag
    22381 gcttcagtgg ctctcctgga ttggtcgtaa tgtaaaattc gatttggtca aatccatcat
    22441 gattcaattg tctattttct tcttttaaga aacgtacctc aaactcagtg ttttccatat
    22501 acagactttt caattcatgc agaatggatt tttccaattc ttttgcgatt tttttacgcg
    22561 cttcatgaag atctgcggct tgttgctgaa gtagaagctc tttttgctct agttcctctt
    22621 tcattttgtc caattgacct tctgaaaaat cagaagaagc tagctcttcg gtgatttcgt
    22681 catagtaagc caatattgac tcaatggatt cgccgtattt acgtttcaac tgtcggatta
    22741 attctaaacg ttgcgtaact tcttctaaac gactctcatc taactccaat aaatcaatct
    22801 gacgactcat atcgccgatt gcatcttgca gcaaatagta agcactttgg atattttcat
    22861 aaattgcttc atactccaca tctagatggg cgatcccttg cagttctgaa acggccaagc
    22921 caacaccatc tacactgttt tgttcattat tcgtcagtgc tccgtaacct gcagctaatc
    22981 catcagcaat tttttgataa ttgctaagtt tttctctttc ttcttttaat ttctcttctt
    23041 ctccgacttg caaatcagca gcagctattt cttcttgctg gaaatggagc atatctattc
    23101 tctgaacata ggatttttca ttttgctgca cttttcgtac ccttttttcc agctcacgat
    23161 aagagatata ttcttgctga taactctttt tcttctgttg gaaagctaca tctccaaaac
    23221 gatccaataa agcaagatgt gattcaggct ggagcagttc ctgatgttcg ttttgtcctt
    23281 ggatatctac taaataactg ccgattcttc tcagattagc taatgtgata atatgaccat
    23341 tcactcgaca aacatttttt ccagttaagg acatatcccg tcgaacaatc agattgtcct
    23401 catctgtttc gatccctaat tcaaccatca gttcagagaa tccttcttgt tttggtagtt
    23461 caaacaaccc ttcaagcaca catttttctg ctccttgacg tatataatcg cttgaacctc
    23521 ttcctcctgc taacagacct aaagcatcga taataatgga tttacccgca ccagtctctc
    23581 cggtcagcgc ggtcattcct tcgtgaaaag aaagccgcaa ttccggaata atcgcaaagt
    23641 tagtaatact gatttcttgt agcatcacat tcgctcctca acttaaaggt aatgcaggaa
    23701 gtctttcttc aataaaactg cctggtcttc agttcgtgta atcatcaaaa cattatcgtc
    23761 atcattgata atcgaaaata attctttctt gtaacgttta tcaatcaaat ttgctgctgc
    23821 cgaagcattt cccggcaatg ttttcaaaat aacaaatttt tccatttggt caactgccac
    23881 gaaagcgtct tttaatagtt tttccaattt agtatttaca tcttcgtttg tttcagctgg
    23941 tatgctataa cggtagccgc catctgaagc cggtaccttg atcagtttca gttcctttat
    24001 atctctggaa atcgtagctt gagtaacaga tattcctttg cttttcagga tttcaacaaa
    24061 ttcttcctgc tttcggatat cttgctcact caacaatcga gtaatcaaac gatgccgatc
    24121 tttttttctc attacgtctt cgctccttag catattcttc ttaatcaatg atagcttatt
    24181 tccttggtat aataaagaag aatccgcata cttctcactt gattttcaca gttacaattt
    24241 atttatggtc aaactgttcg tgtgcatttg aaaccacttc tgccatatta atagctgaat
    24301 tgattgttcc gctttcgggt actttcctca agtgagccaa gaactcgata tttccttctc
    24361 cccccgtgat cggagaataa cttaattcca gcacatcata tccaccttgt gccgcaaatg
    24421 acaaaatgtc ttctaaaact ttttgatggg tctgagggtc acgaacaatt ccgtttttcc
    24481 ctacagcttc tctgcctgct tcgaattggg gtttgattaa agcaatcact tctccctctt
    24541 cttttaaaat agcatgtaat ggaggcagca tcagttttag tgaaataaaa gaaacatcga
    24601 taacagcaac ttctggaatt cctaaggtaa agtcttcggg ttttgcataa cgaaaattca
    24661 ctctttccat tacgacgact ctaggatctt gtcttatttt ccaagctagc tgattgtagc
    24721 caacgtctaa agcataactc atttttgcgc cattttgcaa agctgcatca gtaaatccgc
    24781 cagtggatgc accaatatct aacaatgttt ttccttcaac agatagatca aatgctttga
    24841 gtgctttttc taattttaat ccccctcgag aaacataagg aagtttttta cctttgactc
    24901 ttaattcact actcactgga attttttctc ccggtttatc aaaccgctcg tttttttcgt
    24961 tgtaaatcaa accagccatc actgaacgtt tggcttgttc tcttgtttca aataatcctt
    25021 gtttgaccgc taatacatct acgcgttctt tttccattct ttccctcatt tctccacatc
    25081 aaattgcttg atcatctggc gcatcagccc gccatcaaaa gaaaaaacgt cctcttctaa
    25141 tttgtctatg atcttgttgg cggataacag ctcaatctcc aacgcctcac gtgtcttttc
    25201 taggcctaat aaacgcgggt aagtattttt ttccatacgt tcatctttac cagctgtttt
    25261 ccctaatgtt ttcgttgtac caatcacatc gagtagatca tctcttatct gaaaagctaa
    25321 acctaaatgt tcggctaatc gttgaagtag caataagatt tcttcaggtt gcttcgctaa
    25381 tatgccacca gccagcaagg cataacgaat caaatgccct gtctttcttt catgaataaa
    25441 tgctaattct tctaaagaaa gctttttcga ttcgccctcg atatctgcag cttgcccagc
    25501 aaccatacct tgtgatcccg cacaaacagc taattgctga agcagcagaa gcttaggaga
    25561 attacctaag tgtgccatac tgattagctg gaatgctcct gtaagcaaac catctccggc
    25621 caatatagct aatgcttcgc cgaatacttt atgattggta ggttttcctc tacgaagatc
    25681 atcattgtcc atcgcaggca aatcatcgtg gatcaaggaa tacgtatgca ccatttctaa
    25741 agcagctgct acttgataga cgggaacatc aataggctca tcaaaggaag caacggccgt
    25801 taaaagtaat aagggacgta ttcttttccc gcctgctttc actgaataac tcatcgcatc
    25861 atagagtcct ctatccgttg tatgctcatc taaaaaagac aagatttctt tttctacagc
    25921 aggtagatga atagctgaaa agtcagttaa tctcatctca gctttcctcg tttccgtcaa
    25981 aatcaacttc ttcatttgat tctgtcatca ttttcgtcaa ggtctcttct gctttagcta
    26041 atgtatcttg gcaatgtttg cttaattcca ttcctcgttt aaacgaatct agagctgatt
    26101 ctaatggaac gtctccttgt tctaactgca tgacaatttt ttctaattct tgcagtgact
    26161 cttcaaaagt tggattttcc atatttttat tctcctttgt ctatctcttg tacttgtcct
    26221 ttaacgattc catcttgata atggatcgtc agcaaatccc cttgatgaat ctcggtgaca
    26281 ctttttacta cttgatcatt taacgtcgta tagctgtagc ctcttcccat gattttcaat
    26341 ggactcaaca aatcaagaga ttgaacagct tgagcaaaac gctgctgctg ttgtttcata
    26401 tattgttgga ttttctcttc taagcgtttt tctagataag cagtttcttg gcgagctgct
    26461 ttaactcgat aaagcggagc cacttgttcc aattggtggc ttaatgcgat cgtttgtttt
    26521 tctttcgcat gataaatttg ctgtgtagct tgatataatc gctgtttcaa ctggtctaat
    26581 ttgatggtct gaccttcata caatctttca ggctgccgaa atacatagga acgtcgagca
    26641 cgctcgaacc gttcttgttt gcgctggatc tggcgtacaa acgcctgctg caatctagct
    26701 tgccgttctt gaatgcgcat caactcttct gacaaaacag gaacagctaa ttctgcagct
    26761 gctgttggag tggcagcccg aacatctgct actaaatcag cgatcgtggt atctgtttca
    26821 tgtcctacag atgaaatcac cggcgtatta gcttgataaa tcgcccgagc aacccgctct
    26881 tcattaaacg gccataaatc ctcaatcgaa ccacccccac gaccgatgat cattgtatcg
    26941 aaattgccca tctcttctac tctctggata ttcctgacaa cattgtccgc tgcttggttt
    27001 ccttgtacaa ctgttgggaa caaaaccaat tggacaattg gatatcttct tttcaccgtc
    27061 gtgatgatat cgcggataac cgctccgctt ggactggtaa ggaccgcgat tcgctttggg
    27121 aaacgtggca gcattttttt aggcccttga aaaaggcctt ccttcatcag cttttctttt
    27181 cgttcttcca attcttgata taaagcacct attccatcag gctccatgtg ctctacatag
    27241 atttgataag aacctcccgc ttcatataat gaaatacgcc caacgacaag gactttcatt
    27301 ccttcttttg gctggaatcg taatttttgg aatgctcctt tgaacataat agcagatatt
    27361 tttgcatggt catcttttaa gctgaaatat tgatgggcat tggcacgtaa acggaaattc
    27421 gatatttcgc ctgttaaata tacgcgttct aaataaggat cggcatcaaa ttttcttttc
    27481 aaatacttcg ttaatgtagt gacagttaaa tattcttgtg ccatctattc acctgctcca
    27541 aaagcttcgg cagattttac ggtttgatac atcagcatcg tgatagtcat tggccctaca
    27601 ccctttggca caggagtgat ccagcctgca ataggctcca cttcatcaaa tttcacatca
    27661 ccgatcagct taccgttagc atcacgattc atccctacat ctatcactac agcacctggt
    27721 tttacgaatt cttttgtgac aaaatgtcca cgtcctattg ctacgactaa aatatctgct
    27781 tcttgtgcca gttcagccag attttcggtt ttagaatgag caacagtaac agttgcatct
    27841 gccatcaaga gtagctgagc catcggttta ccgacgatat tgcttcgtcc gataactact
    27901 gctcgtttac ccgccggatc gatagaataa gcttcaaaca ttttcatgat gccgtatgga
    27961 gtacaaggaa tcttgtccgg tttacccgca aggagtctgc ctaaattcat cggatgaaaa
    28021 ccgtctacgt ctttttcagg gcggatagct aacaggatct tttcttcatc tatatgcttt
    28081 ggcaatggta attggaccaa tataccatga aaactaggat cttgattgta tttttcaatt
    28141 tctgcaagta gttcgtcttc agaaatcgtc tcaggataac gctctaccaa tgaatggatt
    28201 ccgatttctt ttgctacacg ttctttattt cttacataga tttggctggc tggattttcc
    28261 cctaccaata aaacgactaa tccgggatgg ataccattat ctttcaattt ttggattttt
    28321 tcagcaatct ctgcctgcat tttttcagct aattcttttc cgttaatcaa ttctgccatc
    28381 taaccactcc cagatatttt ttgtgttttc attctaccat agaattttcc cgatttataa
    28441 caggtttatg atgattcata gaaaaaagct aggacttacg ccccagcttc taattctttc
    28501 aatacattgg ataaaacacc gttgacgaat ttacgtgaac ggtcatcact aaatgttttt
    28561 gccaactcga ttgcttcatt taaagcaaca gccgctggta catctgtcac ataagtcatt
    28621 tcaaaaatcg cgatacgcaa gatcaccaaa tccatttttg agatccgatt gatgtgccaa
    28681 ttgtttttta aatgtttttc aatcacttta tccaattcat ctttttttgc acagacaccg
    28741 ccaactagcg tatcaagata aacaggaaca aactctgatt catcttcgtt gatcatgtcg
    28801 cggtggtcta attcgattgc gttgaaaatc gcatcttctt ttgttaggtc tgcattgaag
    28861 tctaatggaa ataaagcctg caatgccatt tcccgaatct cgtgtcttga taattcttta
    28921 ttcattctct tcctcttcgt cttcaaaaag ttcttcgatt gttggttgtt ctaatttttc
    28981 aggaacaaca gctactacat ggatattgac ttcagataaa tcgatatccg tcatgaaaag
    29041 gacttgctgg ctgacccgtt cctgcatttc cattgctact ttaggaactg aaacaccata
    29101 ttctagataa cagtaaagat ctactttcaa gccatcttcc tcgtttgcta agtaaacacc
    29161 ttttccgtga gctgcacgtc ctaataattc agtgacattg ctggcaaaag ttccgcgcat
    29221 tccgtatacg ccttctactt tagaagctgc aatcccgata attacttcga tgacttcagg
    29281 agcgatcacg atctctccta aatcttggtt tgcgtttaaa atcaaatttt tttcatctgc
    29341 catttattta ttcctccctt tgcctaagca cgagatacat ccgaattctc tatgatgcgg
    29401 catagtgatc acgtggtaag aaaactgccg ccgatcagct gcaaactgct atgaattgga
    29461 gaaaacaccc aactttatta tctcttacag cttaaaagtt cttctatagt gtatcattaa
    29521 atcgagtgat tttctataac aaagtctgtg aacactgatt tttacgctta cttcttctat
    29581 tgatgaaact ataagatgat caattctttt ggtgaacgag tcaacacacg atttccatct
    29641 tctgtgatca acaagtcatc ctcaatacgt actccaccga ttcctggtaa gtagatccct
    29701 ggttcatcag tgatcacatt tcctggtaca aattgctttt ctgcacgaga agaaacgttt
    29761 ggtccttcgt ggatctctaa accgatccca tgtcctgtag aatgtccaaa tgcttccccg
    29821 taaccatgtt ttgaaatata atctctagca actgcatcta attggacacc agtcattcct
    29881 ggttttgcag catcaataac tgctaattga gcatctaaga ctatttgata aatttctttt
    29941 aatttttccc caggatcacc aatagcgaat gtacgtgtca tatcggaaac gtacccttcg
    30001 taatagcaac caaagtccaa tgtgatcaag tcaccttgtt cgatgatttt tttgcttgct
    30061 acaccatgcg gcatagccga acgcaatccg ctagcaacga tcgtatcaaa agaaacacct
    30121 gaagcgccaa gagaacgcat ataaaagtct agttgatttg cgacttcaat ctcagtcatg
    30181 cccggttgga tcattttcag gatgtgatca tatgccatat ccgcaatatg acatgctttt
    30241 tcgataatag cgatttcttc ttcatctttc acttctctga gttcttcgat catctgtgaa
    30301 acaggtacta attccgcatc gatgatttct tctaaaaccg aatattctaa aaatgagact
    30361 gttgtttcct caaaagctaa tgagtgcagt tcttctttgc gtactaagtt tgctacctca
    30421 tcaaaaatag gtccgacatt tttaataatt tcaaaccctt gagcctgcgc tgccgcctgc
    30481 tcggtataac gaaaatcagt aataaaaaat gctttttcca aagtaataac agctaatcct
    30541 gtcgtacctg taaagttcgt taagtaacga agattgtacg gactggtaac caaaaatgaa
    30601 tccaaatttt cttcttgcat ttttttgcgc aatttttcta cacgtagcat cataatgaaa
    30661 ctccctcttt ctttgcacgc ttttttccat tatagcaaac ttttcttcgt acacgacagt
    30721 taaataaaaa tttccggaaa acttcttttt attttaaata aataaagcca aaaaggagtc
    30781 acagtctcgc tgcaactcct tttagtttac tattttgtaa aaacttatga tcgttttcca
    30841 aagaaaaatg agacaactgc taccaaaatc actgcgccta aaatagatgg aataagtgcc
    30901 atacctgcag cttttggtcc ccattcgccg aaaatcgctt gtccaatact tgaaccgact
    30961 agtccagcaa tgatattcgt tacacagccc atcgaatttc ctttactggt gatcgcacct
    31021 gcaattgctc cgatcacggc tccaacaatc aatgtccata accaactcat aatataacct
    31081 ccttctttgc taatcaattt tagcatatgg gatttaaagg ggacaaataa ttggttttgc
    31141 tatttcatat tttcttctgc aaagtaaaaa aataaagaaa acctcctctt agaagttttc
    31201 tttatttttt acttgcgttt aaacatacta cgaataccac gtttcactac atcaaagaaa
    31261 ctcaatgatt gtaccattct atcttcatct acatattctc tgatcgcacg aagcactttt
    31321 ttaggttctt tggaagaaaa tgtgtatgta ccattttttt tcgtttgaat ggcatatcga
    31381 ggaatccatt ttccctttag catgactgat gctagtacat aatccacttc ttcccatgga
    31441 atttggatat atttgcgtct atctcgcgca tggtaaaact caaaaccatt attacctatc
    31501 atgatttgcc cgtaatctgt cagacccgta aacgaagtag catcaattac caagtctact
    31561 tttgtattga ttgattgaac cattttctcc ctccagatat ttgtttatga acagtatact
    31621 atataaaaaa agctcgggac aacgcccgag ctatattttt atggattata aaagtccgat
    31681 gacgtgtcca acaacaccga ctacgaataa accaaggatg ataacgattg gagaaacctt
    31741 tttcttaagc aaccacatac ataacaatgt taatcctaat gcagctaaac caggaatcaa
    31801 ttggtctaag ttgtcttgta atgttgttac cttttcagga gaaagggcta agccattatt
    31861 aacttgttca aatgcactct tgattccttc accacctgaa ggcaatttat cccattcgat
    31921 gtaagcacct ttatctaatt ttacagttga tacaactggt aagaatttga ttgataccca
    31981 tctttgtaca agggcagcta atacgaacat ccctaaaatt gaagcacctt ttgttacgtc
    32041 ttgtaacaat ccgccagaaa ggtcatcagt aattttagaa ccagctttgt agccaaattc
    32101 ttgtgtatac cacatgaatg cccaacgaat aatgttccaa cctaagaaga agatgattgg
    32161 tccaaggata tttcctccca tagctaatga agcacctagt gcacctagca ttggacgtac
    32221 tgtaaaccag aacactgggt caccaacacc agctaaagga cccatcatac ctactttaac
    32281 cccttgaatc gctacatcat caacaggcgc gccattcgca cgttcttctt ccagagccaa
    32341 tgtaacccct aaaattggag aagcaatgta tgggtgtgta ttaaagaatt ccaagtgacg
    32401 ttttaatgct gcagaacgtt cttcttttgt tttgtataat tttttgattg ctggaatcat
    32461 agaaaatgcc cagccaccgt tttgcatacg ttcgtagttc catgaacctt gaatgaaggt
    32521 agagcgccac gcaacagcta aacgatcttt ttttgataat ttgatttttt cttctgccat
    32581 tttattttcc tccttcaaga ttaatagtca ttcaaaatgt cgcctaaagg gtcaccagag
    32641 ttgcttccgc ctccattatt tgatgaacca cccattttag aaaggttcaa gtaaataaga
    32701 gctaatgcaa cacctaaagc acctagagca atcaatgtta attgagaaat tgcagctaca
    32761 acgaaaccaa taacgaagaa tggccatact tcttttgtag ccatcatgtt gattaccaaa
    32821 gcgtaaccta cagctacgac cattccccca ccgatagcca taccatctgt taaccatgct
    32881 ggcattgatt caaggaatga ttgcacagtt tgtgctggga taaatagcaa cgctgctgct
    32941 gggaccgcga tacgaacccc ttgtaaacat acagcaatga tgtgccacat ttcaacctta
    33001 cggatgtttc cttcttcagc agccgcatcc atcaaatgaa cgattggaac agcgattgta
    33061 cgtacgatca ttgttaagaa caaccctgca acagccaaag gaacggcaat cgcgatcgca
    33121 gaaggaacac ctttaacacc ttgtccacct aaaactaaaa taattgctga tgctactgat
    33181 gctaatgcag catctggtgc tacagcggca ccgatatttg cccaacctaa agcgatcatc
    33241 tgaagcgtac cgccaagtac gataccagct tcgaggttac cggtaactaa accgatcaat
    33301 gtacatgcca ctagtggttg atgaaattga aattcatcaa gaataccttc cataccagct
    33361 agaaaggcaa ctaatacgac taaaattatt gaaataatag acatgataag cctcctataa
    33421 attttaatat tatttattac gcttttgcta attcagcttt tgcttttttc aaaatatcat
    33481 ccatgttatc gcgtgaatca ttaggtactt tacgtacatc gaatttcaca cctttttgtt
    33541 caagcttctc aaacgcttca acgtcttctt gtcccataga caatacttta cttacaacga
    33601 ctttaccgac cgaatgcgcc attgacccaa catttaattc tttgatgtct acgccacctt
    33661 caacagctgt caatacgtct tcaggatttt caaataaaag caatgctttg gtatttccaa
    33721 aacgagggtc ttttgctact tcgatcattt tgctaatagg aataacattt gcttttacac
    33781 ctggaggagc agcttgttcg attagtctct tacgcagatc atcctttgat actgcatctg
    33841 aaacaacgat aatacggttt ggttgcactg cttttgtcca agctgtcgca acttgtccat
    33901 gtaacagacg tgagtcaaca cgtgctaata catatttgat ttttccgtct ccgaccacag
    33961 ttccttctgg aagtgctcct ttaggttgtg cgtcttcaac tgctgctttc ggtgcttctg
    34021 ccggttctaa ttcttccggt tttactttta caccgtcttt tgctgtttca atgatgtgtg
    34081 ctgcaatttc atgagcagat tccattgaaa agcgtgatgc gtaggcttca atcaacattg
    34141 gtaaattcaa accgctgacg attgcccatt tatctttgtg ctcttcgaat aatgtgtttg
    34201 attgattgaa cggtgttccg ccccataaat caactaagaa taacacttcg tcttgattat
    34261 caaaagaagc aaccgcttct tccaatttgg ctctcaagtc atctggacct tcacttggtt
    34321 tcaaaatcac ggctttgacg ttttcttgtt cgccaaagat cattgaaccg gattgtaaga
    34381 ttccttcagc aaattgtcca tgacttgcta ggataatccc taccatattt atacctccta
    34441 tttacttttg ttgtaacaaa ttcaaaaata ttaataattg ctaaccatta caacagaatt
    34501 atttgacctt ttccaacaac cattcttgct acaccttttt gattttcgct agtagttcca
    34561 tcaaaaggat tttcctgtca gcaggtactt ttcgaatctc taactccact tcttgactgt
    34621 tcaagtaacg aaaggaatca atatcttgtt gatttactga aacaaaattg gtaatcattg
    34681 atttgccttc agtaaaactc ataccgccga tattcaacga cttcaactgt acaccgcagc
    34741 ggacaaccgc ttcaacatct tggggattgg tgaataaaag catcactttt gctcgtgata
    34801 ataatccact atgatagatt tgaatcattt tctgcacggt tattacattt gctgtgatcc
    34861 ccggcggtgc agcttctttt agtagaaact ttctcaattt atcttgagcg actacatcgc
    34921 tgatcacaat gatacgttca acacctgttt gcttcgacca aactgttgcg acttgcccgt
    34981 ggattaaacg atcatctata cgtgccaatc gaatatccat ctgcaaaact ccttcttcct
    35041 cactacttca cagaaacccc tatcatttct gtgtgaacaa ccacctccta gcacacacta
    35101 ctataagcaa aaagcgtgcc aactttttta cgatacacta attgaattta aaggattctt
    35161 aaccaataca caaaaacgat acactataaa agtgtatcgt ttttgtgtat tcatggcaca
    35221 gtgtattctt ttttatctaa ctgattatgg actagacgta gcagataata catttcttct
    35281 atcggcactt ttacttttat ctgatcttcc aacagaacaa ctgaggctgc caattggcta
    35341 taaagaggat cattcgttgt atttgtcaat tcgttttctt ccacagtcaa tggttcattc
    35401 cgaataatac gttcgatcat tcctgctgtg tgcatcacta ggttgatttt gaatccatat
    35461 tcttcatcgc cgattcccgt cgtctgagag atttgtgtag aaaattgcca cattggatcg
    35521 attaacttac taccattaat aaagatgaag ttatcagaga taaactcaac acacgtattt
    35581 ttagctgact cttcatctag ggagatgaat tcagtttcct ccaattctga ttccatcagc
    35641 aactgatcca aaatgacttc gatattttga tcaataaaac gttctaaagg aataaatggg
    35701 gcttggatat tcaaatcagt aatcccagta gtagctatga tctgatattc tttttggata
    35761 actggcagtt cttctttcac ttccacaatc gacaaagcta gaacttctaa ttctgtttct
    35821 tgacgcttgt ttaccgctcg ttcgatcaat tctttgattc tttgtgctgt accttcgcca
    35881 gatgcgcaga tagccaaaac agcttttttc ttatgaatag gttttgcttc ctgaacagat
    35941 tctacagaag atatttctgc gtaacctcga aaatttttca atgattcatg caacatatct
    36001 agatctgtcc ctaatactga gacctttcgt acagtttcca aaactagaga agttgttacc
    36061 atatcaacag tacgtactgg aattccagtg tctctttgaa tttgcgaatt aaacgacgca
    36121 agggacccca tatctactaa gagtatgacc ccgcttcctt catctacttc ttgtacattg
    36181 cgttcaattc ggtccaatgc tgttttcgga tccatatcca aaggcatgtc tactgctcgt
    36241 acgttctctg catctaacaa ctgttggaca acttggacca tactgcttgc tgtgctgttt
    36301 ccgtgagctg caacgactac accgattctg ccgcttgctt gatttgcccg taacgagacg
    36361 agtagaacgg tcaagtaata gtcttcactt tctggaatct ccacttgaaa atacgtttcg
    36421 atataactac gcacttcttt cgcaacagca tattcatctg gaaaatcaaa agccatctca
    36481 cggatgttat cattcgtatg ccgttcttct cctaagttga tttttttcaa aaaagaactg
    36541 atatgcaagc tcatagcata aataaagttt tgctgaaact catatggtaa agatcttttg
    36601 accattacat aaatctgatt ggtcacttct atgattttcg gatctacaaa ttctgctaat
    36661 ttgctgtctg cattaaaaga aaaaccatgg tctttataga aagacttcag atggatgttg
    36721 atatctgtag aaatgaaatg attgattgct tcttgatcca aaccatccga cttcagtaaa
    36781 gctgctttat ctccgatgat atcatataga ttataaggta actcatagga gtccgattgg
    36841 attttcatga tcgtatcatt tggtgctaca gttatctttg gctccaatat tttagaaagc
    36901 tctgccattg cactgcggtt gcttgctaat tgaatcaatc cgctacgaat cccttctgtc
    36961 aaatcatcga ttgtgatcga gatttcgggt gattgcatat gatttagaaa tcctcgtgca
    37021 catactaact ggatatttga cttcaattgt cctatatttc cataggtcac actgccgatc
    37081 aatgcttttg ctacatcttc tgtcaaagaa atctttcgct gcgtgcggtt tgcttcgtgg
    37141 gcaatcatga tttttactaa atcgactttc tcatttgcag gacgtttttc aaaagccgga
    37201 agtttgatat tgattgggat acgccgaaca aaagtttcta atagagaaga acctgggtct
    37261 tctgtggtcg cacctacgat ccgtacattt gcttcatggg atttcgttgt ttccccaaga
    37321 cgactgtaag ttccatgatc cataaagtaa aagatcattt cttgaccttc aggcggcaac
    37381 cggtgaatct catccaaaaa cagcataccg ccatccgctt gatcgataat ccccattttt
    37441 tcttcatctg cgcctgtaaa agcacctttt gcgtacccaa ataaatgact catcaaaagc
    37501 tcagggttat tggcataatc agcacaattg aaaacaatca gttccgtttc ctcatctatt
    37561 acatgatgcg cttttgcaaa atgatacatc gcgtgtgcaa aataagtctt tcctgatccg
    37621 gtaggtcctg taatcaaaca atttaatcct cttggcggat acaaaatcgc agcttttgcc
    37681 tgttccactg aatttttcat gctgccgtta gctccaatga tttttgcaaa gatatcctta
    37741 gtatctattt ggagtgtatt tttttcttca gagaccggcg tttctttgta actgactaca
    37801 tgttttgtaa ctggctttac gtgcgtaacg agtgtttgat atatgtaacg tactggtcgt
    37861 ccgtctgtct ttatcagttt tccttcgcgc actagctgat tcagatcttt actgctattt
    37921 gttcgctgaa tccctaacac ttcagcaatt tcctgtgttg ttactccttg atcatattct
    37981 gcctttgtta aatgagcggt tttttctttg acatacatat aaattcggtc aatgcgtttt
    38041 gccaatggta tcgctccttt tctatagact attgtactta ggcttcaaaa cataatacta
    38101 tgtatttatt tctgtttcgt caactattta cctgattttg ttttgttgat aacacgcgga
    38161 tagatagaaa tgttcaattt aaaagaacga aaaatcccga tacgattttt ataatggaaa
    38221 caagaaaaag aacactgttt accatataat tatttcactg atagcaaaca atatagcttc
    38281 catcaattga aaacgatagt tatcaaaaca tcctgattcc aaattggcaa aaaaatatta
    38341 agtgtaaaaa gacaaagtaa acttcgtagc ctccactaca acttttattt ttataaacaa
    38401 aaaaggctgt gacaaaagtc ttgtcacaac ctctttaatc ataagattca attaagcttc
    38461 ttgagctact ggataaacag acacttgttt tttgttacgg cctttacgtt cgaaacgtac
    38521 tacgccatca acttttgcaa ataaagtgtc gtctccaccg atacccacgt ttacacctgg
    38581 atagattttt gtaccgcgtt gacggtaaag aattgatcca cctgtaactg tttgtccatc
    38641 agcacgttta gcacctagac gttttgattc tgagtcacgg ccgttagatg tagaaccgcc
    38701 accttttttg tgggcaaata attgtagatt catagataac aacatggtct gcacctccta
    38761 tttatcatta attgttttaa tttggatata ttctgagttt tcagcttcta tcgattgtaa
    38821 tcctaataaa agattttcta atagaatctc agcaatattg ttttgttctt gtgtcattcc
    38881 tgaagtgact tcggtataaa gataaccgcc ttcttcttca ttcatttcaa caatcggctc
    38941 aaaaccggct aatgaggcaa tcccgttgac agtgctgatc gctaacgcag aaacacccgc
    39001 acaaacgata tcacttccat aaggaccagc atcagcatgc ccggtcaaag tgaatgaaac
    39061 gatctgacct gcgtcatttc gcttaaatgt tcctttgatc atatataggc cttctttctc
    39121 aaacgctatt aagcgttgat cgcgtcgata accacttttg tatatggttg acgatgacct
    39181 tgtttacggt gtgaatgttt tttaggtttg tatttgaaag taacgacttt cttttgtttg
    39241 ccgtgttttt caacagtacc ttcaacagtt gcacctgcaa cagttggagc gcctactttt
    39301 gtagattctc cgcctactaa gataacttcg tcaaaaacaa ctttttctcc tgcttcaacg
    39361 tcaagttttt caacatagat tgcttgtccc acttcaactt ttacttgttt accaccagtt
    39421 ttaataattg cgtacatgct ataaagcacc tccttctgat atacttagac tcgccatccg
    39481 aagtgacagc atatgctgtg cttattactt gttctgagcg gttgtagctg tggagtccac
    39541 aattacaaca gtactagctt accagaacag caaccgttag tcaactaaat ttaaaatcaa
    39601 aaagtaaaac aaaaattcaa gcaatacttc tgcttttcta tcctcacagt tctaccaaat
    39661 aaaatagtcg cattgtgttt cttttattct tataataaaa gaaaaaggag gagcataaat
    39721 ggaataccca aaagaactga tacaagaagc ttctttaaga atgaacggcc gtcctgtaga
    39781 aggatttatc gctgggcaag gacctcttca tcctaaactg atgctcgtgg gagaagcccc
    39841 agggaaaaca gagattgata cgcatgttcc ttttagcggt caagctggaa aagaattgat
    39901 gcaggcactt tcttcaactg gcttaacacg agaagaggtg tatatcacca gtgctgtaag
    39961 gagccgcccg tatcgcgtga ctcatcggat caataaacgg acacaacagt cagaaactgt
    40021 ttatccaaat cggacaccta ctcgttccga agtatttgcg catgcaccaa tattggatta
    40081 cgaactgatg aaggtccagc caaaattaat tgcgacactt ggaaacattg gtcttcaacg
    40141 tttacttggc aaagaatata cggttaccaa aaatcatggt caactctata cagggcctgt
    40201 cttacaatta acagaacaaa aggatgccta cgaatggtca gaaaaaaact atcgaattct
    40261 tccacttttt catccagcag ctattttcta caatcgctcc cttgtacctg ttatccatca
    40321 agactggcaa gtactagctt cacttttaca gcaatattaa aaagaacaga caagaccata
    40381 aaatcttcag tttacagtct tgtctgttct ttttcattgt tttaatggct aattgcagta
    40441 tatttcgcat gtaaagctgt caacgcatga gcaactgttt gatgttgatg cgtgaatgta
    40501 tgttcccaca agtgagaaag ttgtttttct acttctggct gcaaggaagt atagataatc
    40561 gtttgtcctc tttgctctgc tttttcttga aattcatcaa aaactgcacg tgccgtcaaa
    40621 tcaatacttg gtacgcctcg catggagaaa atgacaccat cgtgatgatt tagttcttcc
    40681 aatttatttt tgagtttttc tgaagacata aaaaacagtg gaccactaat atacactact
    40741 gtccagttat tcagcttttc cgtttctggc agccccattc tttgccaatc gatcgtttct
    40801 aatgaaatcg tgatagcagc gcttttgaca acaaaaaata cacagccgcc gatcaccccg
    40861 atcacgatag caatacttaa atcaaagata actgtacaaa tcatcgtaag gataaacaat
    40921 agaactgctg accaatattt attagtaaat agctccttga tcgtttccca ttcattcata
    40981 cgccaagccg tcacaatcaa aacacctgca agtgctggca ttggaaactg tgacatcact
    41041 ggcgcaaaaa caagcatgga gagcagtaag acaactgcat gtatcatacc agctaatcta
    41101 gtttgtgcac cagatttgat ggccacgctt gtacgggcga tggccgcagt agcaggaatc
    41161 ccgccaaaga aaggaagcaa cagatttcca attccttgcg ccattaattc ttgattgcta
    41221 tctaagggct tatttgccat tcggccagcc gaagcaccac atagcaagct ttcaatcatt
    41281 cctaataaag caatgctaat agctggaact gccacttgtt gcaaggcaga aaagctaaaa
    41341 tctcccatat tcaaacgatt actactgatc aatgtttgtg gaatcttacc cacagttgct
    41401 actggtaaat ggaacaatag cataactgct gttgccaaaa tgatagccag cagcgaacta
    41461 ggcattcgtt tcgcccattt ttttggatag ataaacatcc ctaaaatgac tagccctccc
    41521 atgatcaaag aggctgtgtt tatcggaaat cctatcgttt gataactact tagtttttca
    41581 ataacgtttg taccttccga gtgaacacca aataaattat cgatctgacc taatgcaata
    41641 atgatagcaa tacctgaagt aaaccccgta attacgggtg ctgggataaa agaagtcagt
    41701 gttcctaatc ggaaaagtcc tgctgcaagt aaaaatagac ccgctaaaaa agtagcaagc
    41761 aatacacctt gcattccctt tgttgccgca atagacatca agatagctgc catcgcacct
    41821 gtcggacctg atatttgata aaaaccgcct gataaaccgc caataacgat accggcaatg
    41881 atagccgtaa tcaaacctgc ggccgcatca gctccgcttg ataccccaaa agccaaggct
    41941 aaaggcaatg ctacagcagc tactgtgatc ccagccaaaa gatctttctg aaatcttgaa
    42001 agatcataac ctgaaaattc tttttttagt gaggaaacat aatttttgaa cacctatcta
    42061 actccttttt catgaaattt tctaaaatac cattcattag aatatcatat ttcatgagat
    42121 aaagtaatat ttttcacaaa gtttcctgtt agataaattc ttattttttc tatatttata
    42181 agaaaataga aagcaatgcc aacagagcta gcccaccttg tttgagtaaa atccaacgat
    42241 cgctagtcaa actgccatat aatgcaacaa aaaaaataga tattaatagc atacttgtga
    42301 tttctttaga tgcggaagaa agataagctc cataaagtag tcccactcct aatagtccat
    42361 tgtacactcc ttggttctta aacaatgttt gaacagattt tctgcttaac tcttctttac
    42421 tcattttaaa aaccctactt gtcgtgtcag aatcagttct caccgtttcg atatacatga
    42481 tataaaaaaa ttcaattgca actagagtca ctaaaattct agatagtatc gtcatatctt
    42541 cttcctcttt tctgatgagt tttatttttt tgctccttat aattgtactc gataaggatg
    42601 tttttttatt ttatttgcct ttgaaaacaa acaatattgg cacaaaaaac aaagaaaaac
    42661 cgttagaaac ctcagtttct aacggttttt tagcgtcaca ggagggattc gaacccccga
    42721 ccgtccgctt agaaggcgga tgctctatcc agctgagcta ctatgacaag aatcagc
//
LOCUS       DADVXO010000009        40408 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.009, whole genome shotgun sequence.
ACCESSION   DADVXO010000009 DADVXO010000000
VERSION     DADVXO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 40408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.009"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(200..2197)
                     /gene="tkt"
                     /locus_tag="KB950_000436"
     CDS             complement(200..2197)
                     /gene="tkt"
                     /locus_tag="KB950_000436"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBB6856677.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2299..2550)
                     /locus_tag="KB950_000437"
     CDS             complement(2299..2550)
                     /locus_tag="KB950_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBB6856678.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2697..3320
                     /gene="lexA"
                     /locus_tag="KB950_000438"
     CDS             2697..3320
                     /gene="lexA"
                     /locus_tag="KB950_000438"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBB6856679.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3500..3718
                     /locus_tag="KB950_000439"
     CDS             3500..3718
                     /locus_tag="KB950_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856680.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4007..4519)
                     /locus_tag="KB950_000440"
     CDS             complement(4007..4519)
                     /locus_tag="KB950_000440"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBB6856681.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4533..6830)
                     /gene="recJ"
                     /locus_tag="KB950_000441"
     CDS             complement(4533..6830)
                     /gene="recJ"
                     /locus_tag="KB950_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBB6856682.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6972..7415)
                     /locus_tag="KB950_000442"
     CDS             complement(6972..7415)
                     /locus_tag="KB950_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBB6856683.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7416..8204)
                     /locus_tag="KB950_000443"
     CDS             complement(7416..8204)
                     /locus_tag="KB950_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6856684.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8201..9142)
                     /locus_tag="KB950_000444"
     CDS             complement(8201..9142)
                     /locus_tag="KB950_000444"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBB6856685.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9319..9906)
                     /locus_tag="KB950_000445"
     CDS             complement(9319..9906)
                     /locus_tag="KB950_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBB6856686.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10127..11434)
                     /gene="obgE"
                     /locus_tag="KB950_000446"
     CDS             complement(10127..11434)
                     /gene="obgE"
                     /locus_tag="KB950_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBB6856687.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11728..12738
                     /gene="gap"
                     /locus_tag="KB950_000447"
     CDS             11728..12738
                     /gene="gap"
                     /locus_tag="KB950_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB6856688.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12823..13758)
                     /locus_tag="KB950_000448"
     CDS             complement(12823..13758)
                     /locus_tag="KB950_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6856689.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13944..14903
                     /locus_tag="KB950_000449"
     CDS             13944..14903
                     /locus_tag="KB950_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBB6856690.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14989..16083)
                     /locus_tag="KB950_000450"
     CDS             complement(14989..16083)
                     /locus_tag="KB950_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6856691.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16272..17033
                     /locus_tag="KB950_000451"
     CDS             16272..17033
                     /locus_tag="KB950_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6856692.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17148..17588)
                     /gene="fabZ"
                     /locus_tag="KB950_000452"
     CDS             complement(17148..17588)
                     /gene="fabZ"
                     /locus_tag="KB950_000452"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBB6856693.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17808..18563
                     /gene="fabI"
                     /locus_tag="KB950_000453"
     CDS             17808..18563
                     /gene="fabI"
                     /locus_tag="KB950_000453"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBB6856694.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18673..19686)
                     /locus_tag="KB950_000454"
     CDS             complement(18673..19686)
                     /locus_tag="KB950_000454"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBB6856695.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19851..20771
                     /locus_tag="KB950_000455"
     CDS             19851..20771
                     /locus_tag="KB950_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBB6856696.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            complement(21051..21551)
                     /locus_tag="KB950_000456"
     CDS             complement(21051..21551)
                     /locus_tag="KB950_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6856697.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(21658..22176)
                     /locus_tag="KB950_000457"
     CDS             complement(21658..22176)
                     /locus_tag="KB950_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6856698.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            complement(22177..23532)
                     /gene="rph"
                     /locus_tag="KB950_000458"
     CDS             complement(22177..23532)
                     /gene="rph"
                     /locus_tag="KB950_000458"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBB6856699.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            complement(23535..24356)
                     /gene="racE"
                     /locus_tag="KB950_000459"
     CDS             complement(23535..24356)
                     /gene="racE"
                     /locus_tag="KB950_000459"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBB6856700.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            complement(24518..25174)
                     /locus_tag="KB950_000460"
     CDS             complement(24518..25174)
                     /locus_tag="KB950_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6856701.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            complement(25190..25837)
                     /locus_tag="KB950_000461"
     CDS             complement(25190..25837)
                     /locus_tag="KB950_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6856702.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            complement(25850..26665)
                     /locus_tag="KB950_000462"
     CDS             complement(25850..26665)
                     /locus_tag="KB950_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBB6856703.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            complement(26679..27416)
                     /locus_tag="KB950_000463"
     CDS             complement(26679..27416)
                     /locus_tag="KB950_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6856704.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            27670..28680
                     /gene="trpS"
                     /locus_tag="KB950_000464"
     CDS             27670..28680
                     /gene="trpS"
                     /locus_tag="KB950_000464"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBB6856705.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            complement(28850..31270)
                     /locus_tag="KB950_000465"
     CDS             complement(28850..31270)
                     /locus_tag="KB950_000465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBB6856706.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(31275..32318)
                     /gene="pheS"
                     /locus_tag="KB950_000466"
     CDS             complement(31275..32318)
                     /gene="pheS"
                     /locus_tag="KB950_000466"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBB6856707.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            complement(32627..32968)
                     /locus_tag="KB950_000467"
     CDS             complement(32627..32968)
                     /locus_tag="KB950_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6856708.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            complement(33037..36759)
                     /gene="addA"
                     /locus_tag="KB950_000468"
     CDS             complement(33037..36759)
                     /gene="addA"
                     /locus_tag="KB950_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBB6856709.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            complement(36756..40283)
                     /locus_tag="KB950_000469"
     CDS             complement(36756..40283)
                     /locus_tag="KB950_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBB6856710.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
BASE COUNT        11448 a         8674 c         7051 g        13235 t
ORIGIN      
        1 tggaaagttt tgtctcgctt aatttgacaa ataatattct caagaaacac aaactaatcc
       61 gaagaattct ttgttttgtc atttaaatgt aacatagccg aaaacgaaaa gtaatattga
      121 tttattcttc taacacataa agaacagctg tctccaactt gcagacagct gttcttatca
      181 gtcaaacaat tgcttggatc tatgatagtt gattgtacgt attcacgaca ttttctactg
      241 taaatccaaa ttcttctaaa attttagtgc caggggcaga tgcaccgaaa tgatcgattg
      301 taatcgttgc gccctctgtt cctacgtagc gttcccatcc aaatgatgca gcagcttcga
      361 tagccacacg tttcttcact gatttaggaa gtacactttc tttatattca gaagattgtt
      421 tttcaaagag gtcaaagctt ggcatagaaa caacagaaac atctttgcct ttttcagcca
      481 attctttttg agctttcaca gccaaatcaa cttcagaacc tgttgcaatc aaaatacctt
      541 ctggtgtttc tccttgtgat ggagaaagaa catacgcgcc ttttttcacc atttcatctg
      601 ctacttcttt tgtagaaggc aatactggaa ggttttgacg actcaataca aggatagtag
      661 gcgcatctgt tgtctccatc gctactttcc aagcagcacg tgtttcattt ccgtctgccg
      721 gacgaatcac ttgaacacca ggcatgcagc gaacagatgc tagttgttca atcggttcgt
      781 gcgttggtcc gtcttctccg acagcaaccg agtcatgtgt taagacgtaa acaacaggag
      841 tatgttggat tgctgctaaa cgaactgctg gtcttagata atcgacaaag acaaagaatg
      901 ttccaccgta gatacgagtt ccgccatgaa gttggatacc attcatagcc gaagccattg
      961 caaattcgcg gacaccgaac cagatgttgc gtccttcata atgttcagga gtgaaatctt
     1021 tatctgcagc caccattgta ttattggaac ctgacaagtc cgctgaacca ccccagaagc
     1081 ttggtatagc tttcgataat tcttggatga cttctttact tgaaacacgg cttgcttgac
     1141 tgcttccaac ttcataagtt ggcaattctg catcccagtt ttctggtagt ttgccatcaa
     1201 aagcatcttc aaattgttgt gctaattcag gataagcttt tttataattg gcaaacattt
     1261 cattccatgc atcttcagct ttttgtcctt cttctatcat cgtttgatgg aagcgggcag
     1321 ctacttcttc tggaacagtg aaatctggat actcccaacc ataaacttct ttagccatct
     1381 tgatgccatc ttcaccaaga ggagcaccat gaacagcaga tgttccttct ttaggagcac
     1441 catagccgat gactgttttt acttcgatca atgttggttt gtctgtttca gcttttgctt
     1501 cttcaatcgc tttagagatt gcttctaggt catttccgtc tttcactaag atatgttgcc
     1561 agccatatgc ttcatagcga gcaccaacgt tttctgtgaa agcttttgaa gttggaccgt
     1621 ctagtgaaat atcgtttgaa tcgtataaaa cgatcaattt ccctagtttc atatgacctg
     1681 ccatcgaact tgcttcttga gatacccctt ccatcaagtc tccgtcaccg catattgcat
     1741 acgtatagtg atccatcaca ttaaagtttt ctttgttata cgttgctgca aggtgcgctt
     1801 ctgccattgc cataccgaca gccattgcaa tcccttgtcc taatggccct gtagtggctt
     1861 ctacaccatc tgtatgatgt acttcaggat gtcctggtgt tttagaatcc cattgacgga
     1921 attgtttcaa atcatcgatt gtcacttgat agccagctaa atgaagcaag ctatacaaca
     1981 ttgctgaccc atgtcctgcc gaaagaacga aacggtcgcg gtcagcccag ttttttgatg
     2041 tcgttggatt tactttcagg tgttttgtcc ataacgcata tgccataggt gctgcgccca
     2101 tcggcaatcc aggatgtcct gagtttgctt tttggactgc ttcaatactt aatgtacgaa
     2161 ttgtgttaac gccaagttgg tcaattttgt caaacaaaat aacccctcct attaatttag
     2221 gtttttttta cctttatttc ttacactttc attctataag atgtcacacg aaaaagcaat
     2281 ctttttcagt attttttact aaaagttgtc atttcggttg tgtaagcctt ttttcttttg
     2341 gatttctttt aatttttccg gggtcacgtc attgccctca ggatcgacga ctttcattcc
     2401 ttcgatatga tgacgcatac cggaacgaaa agcttccaaa tactctttgc gcaagttgat
     2461 ttgttcttgt tcttcggctt ttgagagacc attttccttc tttttacgtg caagttcatt
     2521 gatccgctct attttttcag gtgacagcat gctcagccct ccttttactc tccttcatat
     2581 tatataaaaa ctgaaaaaaa aacaactaga taaactaaat aaaatacgaa cattcgtttg
     2641 atttagcgct ttttatatgg tactatttat ttatcaagtg aaagaaggtg taccttgtgg
     2701 cccgacaaac tgaatcaaga caaatcgaag tcttacgata tatccatgaa caagtaaccc
     2761 aaaaaggata cccgccgact gtccgtgaaa tcggggaagc tgttcagctt tcttctactt
     2821 ctactgttca cggtcatctt gcacggcttg aaaaaaaagg gtttatccaa agagatccga
     2881 cgaaacctcg tgcaatcgag ttgacaaaac aaggtttaga aaagattggg attcgtccaa
     2941 caactattcc agtacttggc gtcgtaacag ctggagaacc cattttagca gtagaggatg
     3001 cttcagactt ctttccaatc cctcctaatc tttcttccga agaaggcagt ctctttatgc
     3061 tgacaattcg tggagatagt atgatcaatg ctgggatatt ggatggtgac aacgtgatcg
     3121 ttcggaagca agaaactgct caaaatggcg atattgtcat tgcaatgact gccgaggacg
     3181 aagcgacttg caaacgattc tataaagaaa aaaacttttt ccgtctgcag cctgaaaacg
     3241 acttgctcga accaatcatc ttagatcaag tatctatatt aggacgagta gtaggacttt
     3301 atcgtagtca tatctattaa ttgctactaa ttaaaagaaa actttttaaa aagagtttga
     3361 gacgttggtc tcaaactctt ttttcgcccg ggttcttctt ctttcaaatt tttatcactt
     3421 ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt gttcgcataa
     3481 attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat taactgtttt
     3541 gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg tgtttgttcc
     3601 gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat ctgagcggcg
     3661 cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc aatcataata
     3721 aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg gtgtgacaaa
     3781 aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt tcggtattaa
     3841 gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg tttccattta
     3901 taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg gtcgctttaa
     3961 aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa tattccatta
     4021 atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc aattcgatca
     4081 aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc gtcgctttga
     4141 ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct ggtttaatgg
     4201 catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc acttcgatgg
     4261 tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct aactcataag
     4321 caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata tcaattcgtt
     4381 tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca ccatctgcca
     4441 tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga atactggcaa
     4501 tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt ctttatggta
     4561 ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg tcgctggtat
     4621 aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa aacaccgttc
     4681 tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa taatttttca
     4741 gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat tcgttcttga
     4801 gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc attcaaatag
     4861 gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga ccgaaaaact
     4921 tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt tggttttctt
     4981 ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc ttcctggata
     5041 atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct accttccgac
     5101 gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag accacccaca
     5161 gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt gatcgaaagc
     5221 tttcctacca catctacctg attattcagc agttcttctt cttgagtacc aaaaccaaaa
     5281 gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg ctgttggttt
     5341 gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg aggcaaagga
     5401 ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac tgttacttca
     5461 tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc aatttgatga
     5521 tcgactatat attggttcat cttctcctgt agtatagtga cattttcact tggcatcgtt
     5581 aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat ctggtcaagc
     5641 atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga tccatcttct
     5701 tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc agcaacaatg
     5761 cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt ctccttattg
     5821 accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc ttcattgatc
     5881 tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc aagtaacgag
     5941 acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc aatcgaaaaa
     6001 ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa tagcgcctga
     6061 agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag gattcgattc
     6121 tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc caaaaattct
     6181 gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac tcctgccaaa
     6241 tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata agcgtctggt
     6301 aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc ttgctgagcg
     6361 tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa ctggattcct
     6421 tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc aaatcgattt
     6481 ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat taccgttgca
     6541 ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc actgattaca
     6601 gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg atcatgtaat
     6661 tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta acctctttgc
     6721 cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg gagaaaggat
     6781 tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac tttctcactc
     6841 ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa agaataaaaa
     6901 cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg accatttatg
     6961 aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt tttttgttct
     7021 tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc ctgataagca
     7081 gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt tgcttcttca
     7141 attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg tttgatttgc
     7201 tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc accaatcagc
     7261 gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc aaaacttaca
     7321 ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa aacaaatcca
     7381 agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa aattccgctg
     7441 gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac ttctaaaata
     7501 aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat ttttgccaac
     7561 ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct tggatcagcc
     7621 ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc tacgccaagt
     7681 ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt cgtggcagaa
     7741 taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt aatgatatga
     7801 ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat cattcctaag
     7861 atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa gtcttcgaaa
     7921 atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc catcaccttt
     7981 tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc ataagcctct
     8041 ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat tcttcgtgcg
     8101 caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc gcccagaccg
     8161 cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct tcctcctcgc
     8221 gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca aacacttctt
     8281 gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg atatgagtaa
     8341 gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga gtcgtggaat
     8401 ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca tgtaccagaa
     8461 tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg cctaaaatag
     8521 tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc gtccgcccat
     8581 cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca gctggaatac
     8641 ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca accacacgat
     8701 aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat tgctgatcag
     8761 taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt aaacgcgttt
     8821 gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg taaatctcta
     8881 atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt ccaaaaatat
     8941 gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga atagtcgttc
     9001 gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca ttgcgctcgt
     9061 ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca cctgcacctg
     9121 ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc tttaatttta
     9181 gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta aaaataacgt
     9241 tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa cccgtttgag
     9301 atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt tggaagtttg
     9361 cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt cagcttcttt
     9421 cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt ctccgcctaa
     9481 ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa tcaagatcag
     9541 tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct gggcatcttt
     9601 tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc ctaataaaat
     9661 ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg tagaagattt
     9721 gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc cattaggatc
     9781 atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta actttttgat
     9841 ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga ttactcgttt
     9901 gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt aattgaaaaa
     9961 taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa agtcgtatca
    10021 cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc acaacctccc
    10081 ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat tcgacaaatt
    10141 caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt gcccgaagag
    10201 cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt tcatcatgat
    10261 cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc cacgttgcat
    10321 ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag tgtacagttt
    10381 cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac aaatctgctg
    10441 tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga aatactggga
    10501 tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat ttcactagat
    10561 tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga cgctctagca
    10621 agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca taaggatctc
    10681 gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt tcgatatgac
    10741 gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag cctggcaagt
    10801 ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta ggtacgagcg
    10861 tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat agtaaagtcg
    10921 atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc aattctagtt
    10981 cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca ggattccgag
    11041 gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca actagtgttt
    11101 gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg acagtggttc
    11161 cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct ttactcatcc
    11221 cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc aatgtacgta
    11281 acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg ccagccggtc
    11341 cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg cccttgccag
    11401 ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc tcctattatt
    11461 catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact tattttatcg
    11521 gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct tcttttttta
    11581 tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt gacaaaatat
    11641 tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc gtttcacagc
    11701 acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa cggatttggg
    11761 cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat tgaagtagta
    11821 gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt tgactctact
    11881 catggtactt atcctggcac agttactgca acagaagact caatcgtagt ggatggcgaa
    11941 gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa agaaaacggt
    12001 gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc aaaagcgcat
    12061 ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat gaaaaccatc
    12121 gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc agcaggttca
    12181 tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt tgggatcgaa
    12241 gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt agatggacct
    12301 gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat cccacattct
    12361 actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa attacaagga
    12421 cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc agtattgaaa
    12481 acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt tgacaatcct
    12541 tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac aactcaaggt
    12601 tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca acttgttaaa
    12661 actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg cttactagaa
    12721 aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg actggatcca
    12781 gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa catattggct
    12841 taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg cttccgcatg
    12901 ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat agaccttatc
    12961 taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca ttgggcgctg
    13021 gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag cacggacttt
    13081 tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca tatcgtctag
    13141 ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga ttgctttcac
    13201 attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca tgctgacacc
    13261 gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta tccactgtac
    13321 atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt ttccatgtcc
    13381 tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg cgtaattggt
    13441 catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt cacttttcgt
    13501 ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga tggtccaatc
    13561 gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg atgtctgtgt
    13621 tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag cataattata
    13681 aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc ctaaaaggat
    13741 tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat aaccttgaat
    13801 tagtataatc aaagaatctc tactatgcaa tgttcatttt tataacccgc gaattgtaaa
    13861 attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt ccgacgaaag
    13921 aaaacaaagg agtgtaacca taaatgacct acaaacatct taccatagac gaactgacaa
    13981 tgatagaatc atattatctt caacataata agccggttga aatcgctaac cgaatgggac
    14041 gtgctataca aactatttat aatgtagtca ataagttcaa gcaaggcaag actgctcttg
    14101 attattggca ccagtataaa gaaaataaga aaaaatgtgg tagaaaagtc attcaattac
    14161 ctgctcatga agtagattac attaaagaga aagtcactct tggttggacg cctgacgtca
    14221 ttatcgggcg aaaagaaagg tctgtttcat gcggtatgag aacactttat cgtttatttt
    14281 ctaaaggaat atttgatatt gacacactac cgatgaaagg taaaagaaaa cccaatggcc
    14341 atcaggaaaa acggggaaaa caacagtatc agcgctcaat ccatgatcga cctgataatt
    14401 atcctgattt caattctgag tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc
    14461 ataaaagtgc cgtcattact ttagttgaaa gattatctaa agtcattatc acgattaaac
    14521 ccaacggccg taaggcatta gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc
    14581 ctaaaaactt ctttaaatct attacgtttg actgtggaaa agaattttct aactggaaag
    14641 ccattagtaa ccaacatgat attgatatat attttgcgga ccctggaaca ccttctcaac
    14701 gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg
    14761 attttagaaa agtgaatcag acatttattt ccagtgtcag caatcaacgt aatcatattc
    14821 caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat gtacaagaag
    14881 cattttattc tagcttaatt tgacaaatca taacctataa aaaaacaaaa taaaaaagca
    14941 gaaataactg gctatttcta gcggattatt tctgcaataa ctaattatct attttttatt
    15001 ttcaattaat ttaaagcttg accatggttt caaaaagtca aggaggaata attcgtcatc
    15061 ggagattctt cctacaacat tgacacgtcc gtcgtttttc atatccttca aggcgatttg
    15121 tgtttctcct ttgtattgac cgtacccttc attgtcaatc agtacatccc catgatgcat
    15181 atctcgcgta ttatgaggcg ggaaatcttt atctttgtag tatacacgag tcatcgttga
    15241 acgaaggata tattctgagc gatcccctcg ataactatga aggttatcaa acaagcagat
    15301 tcgttcgttt tctgtaatac cttcttccgt atctactttc aatgttggat aatccgcgtt
    15361 aaatgcttct gccatttctt tcaattcttc ttcacttgcg tacgcatttc caatagaaat
    15421 atcatcaatc aatcctgtaa ggattaaatg cttcacttgt gtcgcaattt caagatctcg
    15481 gtggtcttcc agcgtgcaca agccatcttg cgtaggccaa gggccaaact cagcagattg
    15541 cgaattgacg aatgccatcg tattcaaatt atattttctg aatttttctg aacaaaaaac
    15601 aaagtgttcg tatcctaaac cagagtatcg atgaggataa aaattatgag aacctaataa
    15661 attatccgtg tttggcgaat aactcatgat gttgtcaacg taattcgtgc ccgaactcat
    15721 attgatctcg attttgatac caaaaggatt gcgcgtcatt tttgcttctt ctgctcctgt
    15781 gaatccgata tctaagcgga ccccgtaggc acccatctta tggaaaaaag ataagtcatc
    15841 gtaagaaatc tccaactgtt caaataacgc tggattgata tcaaccatta cttccatgcc
    15901 taggctattc gcgtaatcaa ctacttcttt aaattctgaa agaacttttt ctttatcatc
    15961 attgatctgc agcaaactgg taaaaacccg tttataccca tacttatgag ctaaatccaa
    16021 gtacgctttg tccttttcaa atgttgaccg ttctggataa atcgagattc ctaattttcc
    16081 catattacca tttcctcctg catcttttcg tttttgtatc cacttacatt atagcaaaca
    16141 caaagtaaat ggtctacacc ttttcttttt cttttaaaat aaatgataaa ttccaataat
    16201 ttaagtaggt attttccctc tattttgtta tcattaaaga taacaaaata gtaaattgga
    16261 aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca gcagcattgc
    16321 tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct acagaaagtt
    16381 caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg aacgcattgg
    16441 aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa atggtgacga
    16501 ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg gttgaaaact
    16561 ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc gtcatcaaag
    16621 atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa agcatttggg
    16681 gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt gcgttatcta
    16741 tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt caaaatacag
    16801 atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc gacgcttata
    16861 aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa gtgattgaag
    16921 gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac aaaccaaaag
    16981 aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa taaaaaaata
    17041 acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt ctttttacat
    17101 aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca agcattttgg
    17161 ttgacgatga acgtgaactc gcactcacag acttttttat catctacata tgctgcagat
    17221 aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt cagcacatct
    17281 cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata agctcttttt
    17341 cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag tgtttctaga
    17401 atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa atcttcattg
    17461 attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc tacataatca
    17521 atataacaaa taggataacg attaggaatc aattccatta cttcagtagc agataatacg
    17581 taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt tgactatcaa
    17641 agtatcatat tcctaaatat ttagcaatct ttttttaatt taagaaaaaa tagtttgaca
    17701 atcaaaacat tttgactaat aattaagaaa tcaagaagtt attttagcta ttattctcta
    17761 tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg tcatttctag
    17821 aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct tggggttgtg
    17881 ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat gaacgaatga
    17941 aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt gatgtttcta
    18001 gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg gagatccacg
    18061 gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat gtgtctgata
    18121 tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta attgctgtat
    18181 caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg acttatctag
    18241 gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct tctcttgaag
    18301 ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc aatggaatct
    18361 cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa aaactgatcc
    18421 agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa gtcggtaatg
    18481 cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt atctacgttg
    18541 acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa catataaaaa
    18601 tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt ttttcaactc
    18661 attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta tgtctccaaa
    18721 ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt ccgtttcact
    18781 ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa taacagccgc
    18841 tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa tatctccttc
    18901 aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt tcgtgatact
    18961 tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa ttccaaagaa
    19021 atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat aaacacaatc
    19081 tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt aatttccgcc
    19141 gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata caataaaatt
    19201 cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga cgacaaataa
    19261 atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta aagggcgcaa
    19321 gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt ttaccacttg
    19381 cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc gtctagcact
    19441 ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc cgtcttttcg
    19501 acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg gagagccttc
    19561 atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc cgtctttcca
    19621 tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt tccaatcgat
    19681 ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc atattatttg
    19741 tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc cttttttgaa
    19801 aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt atgttttcat
    19861 tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat caagctgtca
    19921 gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgcc attttggctg
    19981 cctttatcca aaaaagttta tatctgattt ttttgatcgt ccttttttgg attcttcacc
    20041 gtgtgggaaa atatctgatt gataagtctt ttaatcagta ttctaaaaaa gccattctaa
    20101 acgagagccg cttaaagact ttacatagtc ttttaaatac tatttttcat tatacccttg
    20161 gtttcttttt tatctatgca attttatctg ctatcggtgt gccgatcgga tcacttttgg
    20221 ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc ccaagggttc atgagtgatg
    20281 ttattaccgg attttttatt atcatggaac aacagatgga tgttggggac tacatcaagc
    20341 tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg tatccgtacc cttcagctga
    20401 aagcaacgga tggtactgtc catttcatcc cgaatcgaaa catcaccacg atcagcaatc
    20461 tctctcgtgc aaatatgcaa gtacttttag atattcggat tgttcctgaa gaaggatatg
    20521 acaaaatcta tgagatcatt gatcgagtaa atcgtacatt agctgaaaaa taccaagaag
    20581 agctccaaac tgaaccttct atctttggat tagtggatct tggaaacagc aattttgcga
    20641 ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc cttaaaagaa gagttcttga
    20701 gctcctatgt caaagaattg acagcttctg gtttcacgat tccgaacagt ccaattgctg
    20761 tcggaaaata atttggacaa acgatgcaac gaatctcact aaaaagagcg tgggacaaaa
    20821 gtaaagaaca cttttgtccc acgctctaaa cacgtataaa cggcggaagc agaagcaact
    20881 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aattccttct
    20941 tatttcttgg agttaaacgc ttttgtccca gcctcttttt caagataaag tcaaaactgg
    21001 tacgctgttt tttctactta ttttttgatt atcttttagt ttctcgttct ttagctcacg
    21061 atatcgattt gttcttttaa ttcttcgatt ttcggagaca atatattacg acgttctaat
    21121 tcatgcacca tgaagtttac ggctttaagc agctcttttc ttgtaatgat tcctttgaaa
    21181 atccgatctt ctgtgacaac cggtaaaaaa ggcgaatcga ctaacaaatg gagtacttct
    21241 tctagttccc aattttcatc aattgtcgga acatctgttt ctgtcacatc agcaaccgtt
    21301 agtccttcta acgggtccat gctgattgtt tgcaagtcaa tcattttatt gaccacatct
    21361 gataaactta ctagtcctac aaagtgatca tcttttccta atacaggaat tttggaataa
    21421 ccgaccttcg ataacacaag taaaccatgg gacaatggat gatgatacat aacatttgcc
    21481 acattttctg caggaatcaa aaacgtttcc tgattttcca gcagcatttc tcttactata
    21541 ggtccaatca tctgatggcc tccatttcta tttcattcaa cactactatt ttaccgaaac
    21601 cggatgcaaa attcataaaa taaagcttat ttttattaaa aattagagtt tttttattca
    21661 gcgctctctc gaaaaagaaa aggccaactc ttcaactgcc tgatgggcac ggttatagta
    21721 ttgaacagaa atttggtcat ctaaactttc gataagagca tatgatggga tttggatcgg
    21781 gcctctaggc tgagaaatgc taccaggatt caaaaacagg attttctgat ccatctcaca
    21841 gcctatttga tgggtatgac caaaaaacac catatcggca gttgcagctc gagcttcgat
    21901 tgctaactgt gtcaaaccaa atcggacatt cgataaatga ccgtgagtca tgaatactgt
    21961 atcctgacca gtttggatca cttttttctg ttcgaatcct ggtccataat cacaattacc
    22021 ttgaaccact tggaatgtat cccataatgg gtcagaagct tctaattccg aatcaccaca
    22081 atggaaaaag atatccactt cagaacgata acgatctact aagtcaacga ggatatctct
    22141 gtctccatga ttatcactta ctactaaata ccgcatctat ctctctcctt ccagccattg
    22201 tttccactcg gcactcaatt ttttcattgc ttgagcccga tgactgatct tgtttttttc
    22261 ttcgctcgat aattctgctg ctgtttgttt cttaccttct gggataaata aaggatcata
    22321 cccaaagcca ttttcgccag atggtatacg ggctactcga ccgggccatt ccgcttctac
    22381 tacaaggctg tcttttttgg gtgccgcgaa tactaacgta caatggaatt gtgctgttct
    22441 tttttcatcc ggcacatctg tcaattcata taataatttg gcgttattcc ccgcatcact
    22501 tttttgttcc cctgcaaaac gtgcagaata gattcctggc ataccgccta aagcatccac
    22561 tttcaaacct gaatcatctg ctaagactgg ctgatcaagc aattgtgcaa tcgtttctgc
    22621 ttttaatcgg gcattttctt caaatgtgct gccagtttct tccacatctg gcaattcagg
    22681 atagtcaagt aatgttttta catgatatcc cgcttctttg aacatatttc gaaactcttc
    22741 tgcttttcct ggatttcttg tcgcaatgat gattgttttt gtttcttcta tccgctcatc
    22801 attttgagca aactctgtta gcaaagcatg tttttgctct ttgatcaaat cttcaatcgc
    22861 gttttttcca aaaaagagca tttcattcaa ctgttcacca tcaaacgttg cttcttctcc
    22921 agttccttga atctccacga attttccaga ttctgtcatt acaagattca tgtctacaag
    22981 tgctgcggaa tcttcttggt aatccaaatc tgtgacgcaa gtcccatctg gtaagattcc
    23041 tacgctaact gcagcaagat gttcttttat tggatcttct gacaattctt tttctcttaa
    23101 aagtttttca atggctaatt tcaatgccac aaatgcccca gtgatacttg ctgttctagt
    23161 accgccatcc gcttggatca catcgcagtc tacgatgata ctacgttctc ctaatttttc
    23221 caagtctact actgcacgca aggaacgacc aataagacgc tggatttcca ttgttctacc
    23281 agaaagtttc cctttgctgc tttctctacg atttctcgta ctcgttgctc gaggcagcat
    23341 actatattct gctgtcaccc atcctgttcc tgtttctctc aaaaaagggg gaacagaatt
    23401 ttctattgtt gcagaacaaa taactgttgt atcaccaaaa cggatcacta ccgatccttc
    23461 tggatatttg aaaacatttg tttgaagctc cagtttgcga agctcttggg cttttctccc
    23521 gtcatgacgc atccttattt tcctcccaat tcgatatgtt ctacatttaa gtgtccgatt
    23581 ccaagccagt cttcagctat ttcctcgaaa agtttggcag agccagttgt ataaaactgg
    23641 cataatgtcc gaccattttg cggtgaattg ctcaaattga aataatctaa cagcatagat
    23701 acttcaccta ctgtttctgc tccagaatcg atcagttgaa cattttctcc cattacattt
    23761 tgaatgatgg ggcgtaataa tggataatgg gtgcatccca aaatcaatgt atcgattttt
    23821 ttagtggtta aaggagctaa tgtttctgcc acaatttttt tcgccaccga tgaatggtat
    23881 tcattacttt cgacaactga aacaaatttg ggacaagcaa gacttgtcac agtcaattct
    23941 ggtactttct ctttcagtgc tttttcataa gcttgacttt ttaccgtacc aatcgtaccg
    24001 ataatgccaa cttgtttatt ttgtgttttt tttactgccg ctctagtacc gggaaggatc
    24061 acaccgatga ctggaataga aagagcagct ttgatttctt ctaaagccac cgcagttgcg
    24121 gtattgcagg cgatcaccag catcttgatt ccttgctcca ccagataatc cgtcatttcc
    24181 caagtatact ggattacctg ttccgcgggt ctagggccat atgggcagcg tgctgtgtct
    24241 cctacaaata aaatattttc attcggtaat tgtttcaggg cttcttttac tacagtcaag
    24301 ccgccgacac ctgaatcaat aaatccgata gggcgattat ctgtcaatcg tatcatcctt
    24361 tttttcaata aattctatcc tccctattgt gtacttttta gcgtgaattt tcaagtgctc
    24421 ttacttgctt aaaacaatcg aaatctcttt ttttcgaatg gaaagaggaa aagcggttgt
    24481 gactttagtt tgccacaacc gctaaaagaa tcatctatta ataactgtac cgtctctttt
    24541 ccaagtaacc ggaaagcaga gaaagcgcgt aacataccac gaagtacata agtgtcatga
    24601 tgatgaacat cggaagcaca taattggtgt tctgtccata aataatacgt gcctgatgag
    24661 aaagttcagg taaagaaatg attactgcta atgaagtatc tttgatcagt gaaatcaatt
    24721 ggctcacaat tgccgggatc attgatttaa atgcttgcgg taacacgatc gttcgcatcg
    24781 attctacata agtcagtccc gttgataatc ctgcttccat ctgtcctttt ggaacagcat
    24841 tgagtccagc tcggaaaatt tccgacaaca tagctgactc aaaaatcgtt aatgctgcga
    24901 cagcggacca gaagatgttc atctgaatgc cgatttgagg taaggcaaag taagtgaaga
    24961 aaatgatcaa taataatgga agattccgta taatatcaat cactacacca aggattttgg
    25021 aaaagaaagg gatattcgca aaacgaatca cacctgtcag accaccgatc aagaaactaa
    25081 aaatgatcga aaacaatgaa acttcaacag taacctttaa tccttccaat aaaaaacgga
    25141 tattcatcca tgaaaaagcg ccgctaaaat ccatgtattt cccctccttc tatgaacgaa
    25201 ccgcccattt tttctcaaga taaaccatta agtaagataa cggtagcgtg ataactaagt
    25261 aaaataaccc aacgatgata tacgtatcga aggtattgaa tgtttcacta gcgatcaagt
    25321 ctccttgata catcaaatct aaaccagcta cgattgctaa aatagatgag ttttttacga
    25381 ggttgataaa ttggttgcct aatggcggga taacgatttt gaatgcttgc ggtaaaacaa
    25441 tataacgcat gatttctgaa taggtaaaac cagaagatag tcctgcttct gtttgacctt
    25501 ttggtacggt ttgaatccct gaacgtactg tttcagcaat aaaagcagat gtatagatcg
    25561 ttaatccaat cgtacctgct tggaatccat ctagattaaa ccaataaatc ggtacgacaa
    25621 cataaaaaaa catcacgatg attaataatg gaatgtttct aaaaaattct acatatgcct
    25681 ttgctaaggc gctcactact ttattctttg ataattggaa gattgccatt aacgttccga
    25741 taatcaaact aaacacaagt gctaagacac tcgaaaacaa tgtgatcttg aacccgtcga
    25801 caaagattgg tccataagtt tgtatcaatt cgctcattgc ctctccccct tactctactt
    25861 ttccttcagt ttcatttgga aaccattttt gatagatttt atcgtatgtg ccatctgcat
    25921 gcatttcctc tagcgcttgg ttaacagctt tcaagaagtt ttcttgccct ttgttgatcg
    25981 caatcccgta aggctcatta gtaaacgttc caccaactaa ctcatactct ggattttcat
    26041 ctgcaatccc taacaaaatc gcattatccg tagtcatcgc gtctccctga ccagattgta
    26101 aagcagtaaa tgcttctgca tagttttcta attctaaaat ttttgcatct ggtgcatgct
    26161 gtcggatatt cgcagccgat gtagaacctt tgactgctag aaccgttgta gaagcattca
    26221 aatcatctac acttttgatt tggcttcctt tctttaccaa caaagcttgg ccagcatcga
    26281 aatagacatc tgaaaagtca acctgttttt ttcgttcatc tgtgatcgtc atcgttgcaa
    26341 taattgcatc gatatttccg tttttcaata atggaatccg agttttagaa gtgacttcaa
    26401 caaattctgt ttttccattg tcaccaagga tctttttagt gattgctttt gcaatatcta
    26461 catcaaatcc ttgtacagtt ctgctctcaa tatccatcat tccaaataat cgagtatcat
    26521 acttgacacc ccagatgatt tcattggtag atttgcttcg ctctaaaata tcttcatttg
    26581 ccaacccatt tcctttacat ccggctagtg caagaaaagc aatgcctaaa aggaaaatca
    26641 ggcgtatttt ttttaacctg cgcatctttt tcctcctatc aatgattgat aactttactg
    26701 ataaattgct gtgctcggac ttcatttgga ttttcgaaaa agtctctcac atttctgctg
    26761 tcttccaata cttctccatc agccatgaag ataacacgat ccgccacttc tttggcaaag
    26821 cccatttcat gtgtcacgac aatcattgac ataccgtcat gagccaattt tttcataacg
    26881 tctaatacat caccaatcat ttccggatcc aaagcagaag taggttcatc aaacagcagc
    26941 atctcaggat tcatcgctaa acctctggcg attgccaccc gttgctgttg tcccccggat
    27001 aacatggaag gataagaatc tttcttatct aacatattga cagttttcaa aaatttctcc
    27061 gcgtttttga ctgcagttgc ctggtcttgc ttcaacactt tgattggtgc taatttgatg
    27121 ttttccagaa cagttttatt tgggtacaaa ttgaaatgtt ggaaaaccat gccgatgttt
    27181 tttcggactt ctgtcagttt tgtcttttta tcatgcaagt cagtatcatt aatcagcagc
    27241 ttacctgaag agatcgtctc taatccattg atacagcgaa gcatcgtgct ctttcctgaa
    27301 ccagaaggac caatgacaac gacaacttct cctttctcga attcgagatt gatatttttt
    27361 aatgcgtgga acttgccata atatttatcc acgtgatcaa atttaatcat tgccatagaa
    27421 ttacactccc attattattt agtaagattt cttttaaaga tgtataataa aatacattat
    27481 atctgatagt ttagtaattt gtaaagtttc ttagaacgtt tattctccca taaatatttc
    27541 ttccatttct aatctattag ttattttatc ctctttcctt gttttttcct taggaactgt
    27601 tgtttctttt caggaaaaac ggtaagatat taagtgctca gacttacaaa tcgtaaagag
    27661 gtgtcttttt tgaaaaatat tattttaacg ggagatcgac caactgggaa gctgcattta
    27721 gggcattacg tcggttctct aaaaacccga gtggctatgc aggcagatga agacaaccaa
    27781 ctgtttgtca tgattgctga catgcaagca ctgacagata atgctaaaaa tcctgagaag
    27841 gtttcatcaa atgtcttgca agttgcttta gactatttag ccgtgggatt agatcctgca
    27901 aaatctactt tatttatcca atcacaaatt ccggaattag ctgaattaac catgtattat
    27961 ttgaacttag tcagcgtagg acgcgttcgg cgcaatccga ctgtcaaaac agaaatcgaa
    28021 caaaaaaaat tcggtgaaag cgtgcctaca ggattcttta tctatcctgt ttctcaagca
    28081 gcagatatca ctgcattcaa agcgaatctt gtacctgttg gtgaggatca aaaaccaatg
    28141 ctagagcaga cacaggaaat cgtacaaagc ttcaatcata catatggtga agtcttggtg
    28201 gaaccaaaag gcgtattccc accaaaagga atgggtagat tacctggaat cgatggaaat
    28261 ggcaaaatga gtaaatcgtt aggaaacggt atttatatct ccgatcctgc cgatgtctta
    28321 cagaaaaaag tcatgagtat gtatactgat ccaaaccaca tccatgtaca agatcctgga
    28381 caagtagaag ggaatatggt cttcacctac ttggatgtat tcggaacaga taaagaagca
    28441 attgaagaaa tgaaagctca ttaccgccgc ggcggacttg gtgatgtaaa gatcaaacga
    28501 tacctcattg atgtattgga agctgaattt gcaccgatcc gtgcgcgccg agaagaattg
    28561 gaaaaagatc cttcagctgt tatggaaatc ttacgtaaag gtagtgaaga agctgctaaa
    28621 gtagctgcac aaacactttc tgaagtaaaa tcagcaatgg gaatcaatta cttccattaa
    28681 ttcatttact ctaaaaaagc aggctgtaaa ggcctgtttt tttatctcgt ctctgtgcta
    28741 ttcctttttt aaaatcaaaa aaagagatgt gacgaagttg tgtcacatct ttttttcaaa
    28801 tgtatggtct tcaaaagcta cttgatcgtt acttactata ttttccttct tagcgaatac
    28861 ttgcctctag gttttctacc aacgcttttt cgactttttc cattgcttta ttgatttctt
    28921 catctgtcaa tgttgcttct gggttgacga atgtcaggct atacgccatg gatttatgtc
    28981 ctttttcaat gttttctcct tgatagacat caaataattg gatatctgtt aaaaatcttc
    29041 cggcagcaga acggataact tctgttaatt cctgatttgt cacagtttct tttacaagca
    29101 aagcgatgtc ccggctgact gctgggaatt ttgtcactgc ttcgaaaacg atcccttttt
    29161 gagcagcttc cactatagca gataaatcaa actcagcaac atatgtttct ggaatatcat
    29221 aactttttgt aatcgttgga tgcacttgac cgacaaaacc aataaatttt tcgccaaggt
    29281 aaatcgcagc agttcttcca ggatgcattt ccttcatttc tgtcactgtt tgataatgga
    29341 catcttcagt gatgcctagt tgttcaaata agttttctaa aagacccttg atggtgaaaa
    29401 agtctacatt ttgagctttc gtctgccaat ctttttcttc ccaaatacca gaaatagcga
    29461 ttgctgcatg ttttacttct tgcggcagat gtgtacgtgg atcattttct tggaagaaca
    29521 cccgaccaat ttcatataaa gcaatctctg tattttttcg ggcagtgtta taactgatat
    29581 catctaatag tccgctgatc aaattcattc gtaatacaga acgttcttcg ctcattggcc
    29641 agtccaaacg tgtaatatta ctttcattta taacaaactg tcttgatttt tcttctgttg
    29701 tcaatgcata actgattgct tcgctcaatc cgcttccttc aaagattgta cgaattttac
    29761 gagtagtttc ttgttctttt gacaagcttc cagcaaccgt ttctccactt ggaagtgtag
    29821 atggcaactg gtcgtatcca taaatacgag caacttcttc gataatatct gcttcgattt
    29881 ggatatccca gcgtcttggt ggtactacta cggaataaac atactccttt tcttcatagc
    29941 taaatccaag tgcttcaaaa atctcattta cttctttaac gacaagagat gttcctaaat
    30001 attgattgat gcgttcaagt gaaatcttca ctgatacgtc ttctgctttg atctctgagc
    30061 ctttgaccgc gccttgtaga acttccccac ccgcaagaga aacgatcatt gctgcagcaa
    30121 cctcactggc ttctccaaca gtcgcatggt tgattccttt ttcaaaacgt gcagaggatt
    30181 cactacgcaa attaaattgt tttgctgtac gacgaatcga aattggatca aataaagcag
    30241 attctagtgc aactgttgtt gtttcattcg tgatctctga gtctaaccct cccatgacac
    30301 cagctaaagc aactggttct ttgccgttag taatgacgag attgtcagtt gtcaattccc
    30361 gagattctcc atctagtgtt actaatgttt cattttcttt tgcacggcgg acaaagatct
    30421 cttttgattt caatttgtcg tagtcaaatg catgtagtgg ttgtccgaat aaaagcaaaa
    30481 tatagttcgt cacatctact acattgttga ttggacgaat gccttcgttc atcaaaagat
    30541 tttgcagcca ttgaggactt ggctggattc ttactccttc aatgattctg atttgataag
    30601 caggaactgc ttctttatct tcaacttcca cttgaatttt atcgctagca ggacgtgagg
    30661 attctaccag cttttcgtca tcgaatgtag gagtctgacg ataaattgcc ccaacttcat
    30721 aagctacgcc acgcattgac aatgcatccg cacgattcgg tgtgatagat aattcaataa
    30781 tcgaatcatc catatccaaa taaggaaaaa ctggatcgcc atttacagct tcttgtggca
    30841 tataatagat accttcagcg tattctttcg gtataacact atctgaatag ccaatttctt
    30901 gtaatgagca aatcatgcca tttgatactt ggccacgcat ttttcctttt ttgattttta
    30961 cattaccagc gatacgtgag ttaggaagtg caacaatcac tttgattcca accttcacat
    31021 taggcgctcc acaaacgatt tgagacagtt cgtcttcgcc aatatcgact tgacaaatag
    31081 aaaggtgatc agaatctgga tgtggtacgc attccttgac ttcaccgaca acgatttttt
    31141 tcaagccttc ttctggaacc gttactccct ctacttcgat tcctgtcaca gacatcttat
    31201 ccgctaattc ttttggtgtt acattcgata aatctacata acgattgagc catttatatg
    31261 aaactaacat tttcttactc ccccttgaac tgacttaaga aacgtaagtc attttgatag
    31321 aaattacgga tatcattcac tccataccgc aacatagcta cgcggtctgg tccaaggccg
    31381 aaagcaaagc ctgaatattc ttctggatcg attcctgaca tcttcaacac attcgggtgt
    31441 accatgccag ctcctaaaat ttcgatccat ccagtgtatt tacatacgtt acaaccggca
    31501 ccaccacatt tgaaacagct gacatctact tctactgatg gttccgtaaa tgggaaataa
    31561 cttggacgca aacgaatttc acgatcttcc ccaaacattt ttttcattac gacttctaat
    31621 gttcctttaa ggtcgcccat tgtgatgttt ttatcaacaa ctagcccttc aatttgatgg
    31681 aactgatgac tgtgggtcgc atcatctgta tctctgcgga agactttccc tggcgagatc
    31741 attcgtagag cacccttaga gaaatcatgt ttttccattg tccgtgcttg gactggtgaa
    31801 gtatgtgtcc gaatcaatat ctcgtctgaa atatagaaag tatcttgcat gtcccttgct
    31861 ggatgatctt ttggcagatt catccgttcg aaattgtaat gatcagattc tacttcatat
    31921 ccttctacga tctgatagcc catacccaca aaaatatctt caatttcttc catgatttgt
    31981 gtcacgatat gacgtgtacc atgttttact tttctaccag gtagtgtcac gtctattgtt
    32041 tcttttgcca aagctgcatt taacgcttct tcttctaata cttgtttttt tcgctcgatc
    32101 tcttctgtca aaagatctcg gatctcattt gcgaaacttc cgacttttgg tcgctcttct
    32161 ggacttagat ctttcatccc acgtaatact tctgtgatcg gtcctttttt tcctaacgtt
    32221 tcaacacgta tttgatttaa ttcatttaaa tcttgtgccg ctgtgatttt gacgagcgtt
    32281 gcttctttca agctctctaa ttgtgcttgt agagacatgt gcttgttcct cctttaaatt
    32341 ccaaatacaa aacaaaaaga cctcattcct gttaaggaac gaggtcttcg cgttaccatc
    32401 ctaatttgcg gataaatgac ttacaccgca ctcttacttc gttaacgaca attgccggtc
    32461 tgattttatc agacgaactc ggaaagtgaa cttcattttt caaacacagg atctgctttc
    32521 agtctgcgac agatctccct gaactggttt agccaaacta ctctttttcg tcatcgttct
    32581 ttacttattc gtatggacta tagtttacaa gatttttttt acaaaatcaa ccgcattctt
    32641 cctctgtgat ccatttttcg ctccaaactt gtatttgacg catcgctttt tgcaagtctg
    32701 ctcctttttc agtcaagaga tattctgaac ggctcttttc gctacaggca aagtttcgag
    32761 tgactattcc ttctttttct aattccttca agcgttcgac taatactcga tcactgacca
    32821 tcgggatttt tgccgcaagc tcaacaaagc gttgcggacc gttttcgagt agtacatcga
    32881 taatcaatcc gttccatttt tttcctaaaa tagagaatgt tttttcaaat ttagggcata
    32941 aaacgaattc tgtctgtgtt agctccatca tactcacctc ttcccagagt atagcataat
    33001 ccttacaaaa cataagtcaa gtttgctcaa ttataattat ttgttaatgt ttataatttg
    33061 tttcactcga agcaaaatta ggaaaacatt ttctactggc ttattcaaag cttctgacat
    33121 tgcttgttgg taaagtctta gctgtccttg atatttttgg acgattgcat ctatttctgc
    33181 tggatcatcc ggatgtaaaa taaaatctgt tttaaaatca tagatattga gatgatcttt
    33241 ttcttctagg tatccatcga cgattccgtg gattaatagt tcatcttctt gatttggata
    33301 gtcttgaaat acttcgtcag caggaagcag catggaaaaa ggttgttctc gttttacgtt
    33361 ttctttgttg gcaatcagct gttgaccaag ttctgtttga aagaaccata taatagaaga
    33421 aacatctact tttttcgcga ctttttcgtc tactaaccgt ttccttacca attcctgtaa
    33481 tttttgatgg atactctctg tagttggcat ctctaatggc agtaattgta acaaagcatg
    33541 cgtagcagtt ccaacagcag ctgctgacac ttgccgatct ttttgcaaaa attttggttc
    33601 tgctagttgt tcttgcgtat aacgaaattg acgggatgct gctttgttct gactgctttc
    33661 cagagtcaaa cgtgattctt gtgtatcatc tggatcttcg aataatcgtt tgatttcaga
    33721 tacagattga taacttgtgg tttgtgaaga agcttgataa ggatattcat atgccagacg
    33781 tttttgtaac gaatctgcta ataaagtttc atcttctttc atctgctgat ctaagttaac
    33841 cagttctttt tcagcaagca actgtctttg ttcaatgatc gattgctgat tcatccatga
    33901 aatagaaaac tgtgcatttg aatgttgaag aagagaagtc gaaatttctt cctctaagta
    33961 tttctgcatt tcaggatgac ggatcaatcc gtaaccaatc caattcatca agctgcttct
    34021 gcccttcaaa cgtagcgcag gatcaaaaac taactcctca tgatcagctg cttcagacca
    34081 cgtttggatc atttgctctt tatttttata ggaaccgaca atgaaaagct tttgttctgc
    34141 ccgagtcaat cctacataca atttacgcat ttcttccgac aacaatttgt tttgctttgc
    34201 caatttgatg gcttgaaacg gtaacgtatc atataacact cgtgtttcag gatccatata
    34261 gcgaattcct gctccaagtt tttcttcaaa agcataccga ttgcgcaagt cttgtaagtt
    34321 gaactccttt gtcatatcta acaaaaatac aactgggaat tccaagcctt tacttgcatg
    34381 gatcgtcata acgcgaacag cattatcttc tattgaaatg actggttctg ctaagtcttt
    34441 atctttttct tgcatttttt caatgaaacg cacgaactgg tataatcctc tgaaagaact
    34501 ttgctcgtaa gctttggcac gatcaaccaa tgcataaaga ttggcttgtc tctgtgcccc
    34561 tgctggcaga ccgaccacat actctagata tcccgtctca taatagatat cccataacaa
    34621 gtcagtgatc gaagaacggc gagcaagttc tcgccaatgc tctagttgct tgccaaaatg
    34681 ttccagctta gctgctaatt catcttcgtt tgatgcttga taagcaagca cagcatcata
    34741 gtaagtatgt gcacggttag ctaatcgaat gctggctaat tctggttcaa tcagacccac
    34801 gatcggtgag cgcaatacag ccgcaagcgg aatatcttga tagggattat cgattaattg
    34861 tagcagcgag atcatcgtgc gaatttctgt cgcttgaaaa tagttttgag catcattcat
    34921 ctctaatgga atatccaatg ttttgaaaat ctctaaaatc gttaagttgt tcttctttgt
    34981 aggtgtcaag agaacaatgt ctttgtactc tatcggacga cttttttttg attttttatc
    35041 ataaatcata aaggaggaat caattaattg acgaattttt agtcctgtca taaaaagttc
    35101 gccttctgtt ttgtcttcca gtatatcatc cgtcggaatc tcaatctctg attcttcctg
    35161 ttctttttca tagatcatga tttccggctc aaactgttca ttctctggaa agtctgaaaa
    35221 tccttggatc agtttggctg cttcgtcata attgatttga cccactgctg gatccattag
    35281 ttgttcaaaa ataagattcg taaaagaaag gacttctttt ctagagcgga agttctcagc
    35341 caaaacgatt cgacgtccgc cttctttctt tgagaaattc tcgtatttcc cgataaataa
    35401 actaggatct gctaaacgga aagaatagat ggattgtttg acatccccaa ccatgaacat
    35461 atttcctttt gtatcatcgg gttctcttag ccagtataaa atggcttctt gtaattggtt
    35521 cacgtcttga tattcatcta ccattacttc tttgaatttt tttcgataat gttttgaagc
    35581 ttcacttggc agccatacat ctttcgtttt ttctgtgaga atctgcaacg ctaaatgttc
    35641 cagatcattg aaatccagca cacctttttc ccgtttacgc atactgaaac cgtccatgaa
    35701 gctttgcgtg acttttgtca tttcttcgac taacggtagt gctttgtccg ttaattcttc
    35761 catttggctt ggggaaacag gaaaataact tttacttatc tgctgaatca gtttttttgc
    35821 tttatccctc atgcccttga cttcattgga aatttctttc aattctgctt tccgactgct
    35881 cttaaaagtt gaaaaagtta gttgttccaa accagcataa gcttcttcta accgatcttg
    35941 cgagaacgct tcatagatat tcttgatctg ctgttgttct ccagctactt gctcatgcat
    36001 tttttctaat ccttcacctt gtgctaactg agtcatctgt tcatataact gtacacattg
    36061 gtatatatct gccaaaacta aaggacggat ttgtttttga tataatcgag atttcgctag
    36121 cccctcaggc aaacgataat tatcgcttaa ctgttctagc catttttggg ggtcagggtt
    36181 ggcacgggca aactcataaa gagaaaacac cagattcgtc aagccgtcat ctgaacgatc
    36241 attagaaaaa ttcattgtta attggaaaaa tcgttcatca ttttctgcat aaagtgcttc
    36301 tctcagctcg tcccacacat cctccttcat caagatcgtt tctgtttcat ctgttaacat
    36361 gcgaaagact ggatcaatat caattaagta ataataacgt cgaataactg tcaggcaaaa
    36421 agcatgtaag gttgagatat tcgctgtggg caataaaact aactgcttag taaaatgctg
    36481 tctacgtaca gggtcacttt cgctattgac ggattcttgt aatgcctctt gaattctttc
    36541 tttcatttct cttgcggcag cttcagtaaa tgtcacaatc aataattcat cgatatcaaa
    36601 acccattttt agtttttcaa tcactcttcg aacaagcact gtagttttac ctgatccggc
    36661 agacgcagag actaatagat tatctccttg atcaaaaatc gcttgccact gatcatctgt
    36721 gaatcgttca ttttgcggtt ttaaaggaat ttgattcact cttcttcact ccttttcaat
    36781 attctacgca gtgcttcttc cttatctatt ttctccagac ggtggtaatt attttctttc
    36841 agcatcacat caaacgtaca gatgcttctg aatggacaaa attcgcaggc aatccgctgt
    36901 ttatctttat aagctggatt tagtttgatt tctccggaag taatcgcatc tccagctaac
    36961 cgcatatttt cccgattatg cgccatgaat agttgcagtt cttcttccgt gaaaaatttg
    37021 tctttactga aactaccttt ttggtattca tctttcgcat tttttttcac tggatagatc
    37081 agtgattgtt cttttgcttc cagtgaatga tctagtactt ctaaaagagc gggatcatcg
    37141 acaaaaacgc catcataact aaattttttc aatgtattct tctctacatc atctgtctcc
    37201 gtcaatacag gattatgaac atgtaaataa tatgatcctg ctggtttaac agccgccttt
    37261 ccagtcaaac gtaccgcatc catcaatgct acgtccagat acgtcaataa ttgcatagcc
    37321 atgccataat aagcttctgt gacatcgaat gaacgtccgc tagatttgta atcaacgacg
    37381 cttagccaag tatcttcttg ttcagaagct acatcgattc gatcgatttt ccctcgaaca
    37441 tggagttttc caccattact caacggtaat tccagacctg tgataccttt tgctccagca
    37501 atctgaccaa atagaatctc tgtctgaaga ggatgcatgc ctgtattttt gctctgtttt
    37561 tgcaaagccc atgtcacttt ttgaatcgtc tgtcctaact ggtagcggat atagttcatt
    37621 cttgcagaac tatccaatat ctcaaaccga agttctccga atacttcctg cagcactttt
    37681 tcagtaaagt ctctacgctg cgcatcggac atattcacta aggataattg gtttgaaaac
    37741 agtagcttga agaaacgatc caatgattca tgataaaaat caccagttgc tgctggagtt
    37801 agaccataaa cagttcgttc tttcaatttc aaaccaaatt gcatgaagta tttgtattca
    37861 caattataaa aattctccat acgtgaaatt gaagtataaa tctcttttcc ataaagctct
    37921 tccgccattt tttctggcat cgaaacagga acatttaaat gttcctgact ttcaaaaatc
    37981 tgtaaagcaa aatttcgcca tgacgaattc agtactaatt ctttgagctt ttgccagtgc
    38041 tttggtacag gttttttttc atctaaagct aatcgataaa tcctattgac tgtattgatt
    38101 ccgcttctgt aagtccctac ataacaatta gggtcgcttt cgatcgtcaa atgctctttt
    38161 ctctcgacgg gaacagataa atattctgtg attctacgca aataaggaga catcttcagc
    38221 gaaccatctg tgtcatagct ttgtgcataa ctaagatata atcgatctgt tccggaaaga
    38281 aaaacaagat aggcttggaa aggctcaaaa gagatacttt cctttgatgg gtcgaataaa
    38341 aactgtccat ctggcaaggc ctgatttaat acttctcgtt cttcactgga caacaatcct
    38401 tttgtctctt ttcggtctgg aaacacttga tcattcaacc ctaatgcaaa agtgacggca
    38461 gcttgatttg gtcgaactaa ttccaaacga ttgattcgca cttggtcgat tgcggtaggg
    38521 attttaccat aacttagatt ttctagacca cttgtaatga tttcttggaa cagtgaccag
    38581 tcaaaagccg attcaccata aattactaca tattcatcca acagatcaat cagcgctccc
    38641 catgtctgct catggtttct tgccgcttct aagtttcctc tgtctacttc ctgattacgc
    38701 caaaacaata attgttgctg tacgccactt tggatcaaaa attgataaaa aacatttgcg
    38761 gcctcttgtc ctgttttgct tttctttatt tttttcaaaa atactggtac agctttctga
    38821 aataatcgtc taatcgtatt tgactgttct tccaaatact gtacgtcttc aatctgttct
    38881 gcttcaaaat catacgccac aaactgccag tctttttcac gagtccaata agttccttga
    38941 taattgtaag caagtgctac attttcagtg atgtccagtt ttttcctgaa ttcatctcgc
    39001 tcttgtttcc attcatcaag ttcggtttct gcttccgtcg acagaaataa ttcagttttg
    39061 aaaaaacgca taatatcact tagccgataa gaatagcggt caagagcgaa cagactgttg
    39121 atccattcga tcagcggatg attttccatc atttgttctt catcgatata aaaaggaatt
    39181 gccatttctt gaaagatcgg ccgaaggata tttccatata aagacatatt ttttgtcagc
    39241 acttggatat cacgataacg gtagttttct tcactgacta gtcgtctgat ttcaacagct
    39301 aattgtcgga cttcttcagt tggattttca gcttcccaca aatggagatg ctcttgtccc
    39361 cctattttgt gtactgccgc tttcttttgg ctagatgtct cttcccacat tgtttgcagc
    39421 agttgaagct ctgtcggacg agtctgctct ggtgcaaatc ggtccactaa tatagggact
    39481 ttccgttctc ttgcactttg aaaaagacgg aaataaagct ttccagtctc atgaaagagc
    39541 gtaagtactt ctggaagatc cgctgtataa ggacgatcca gcaataaatc gacaaccaat
    39601 tgcccctttt ccatcattgc ctgcagtaat tgatactctt gaccattgat tcgagaaaaa
    39661 ccactgacaa taaatttgac gttgttgaaa tcttgtgtct gcatataatt tgctagtata
    39721 gatagtgcat cttcatttgt tagtgcacgt tgagtcaatt cgctgtcata agctgaaaaa
    39781 atcagcttca agtctttcat tttaagttgc tggtcttctg cattaggtga atcaccaaat
    39841 aaaagcatca ggtcttctgg ttgaatattt cctgtacgca attcactgta gagttcttgt
    39901 aattgacgaa taaaccctct tttattgatt tctccgcgaa agattgttaa ttcttcctca
    39961 tttttttcta aaatctgtcg caagatcatt gcagatcctg attctgataa ttcatttcct
    40021 gtaagaagag ttgtctgttg cagcaaatac caagccagac gataaaaact aaatacttga
    40081 acgtttgttg tgctgaaagt tttttttccg cttcttcttc gcatctccgc taaaatttct
    40141 tgttcctgtt cgaatttgct gtagttggga acaaggaaaa atacttggtt acttgcatcc
    40201 tggtcgagcc aagcggatgc ttcgtcgatc aagacttgac ggtgatcttt tttcccgttt
    40261 ccaagaataa attgtaaaga catgcttttc ctccttcaac acatgatacg gtgctccttt
    40321 attatatcaa aaaatactga acatttgttc tatattcttc aaattatatc cttttaaggt
    40381 tctgatattt ctactttttt aagtatac
//
LOCUS       DADVXO010000010        38832 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.010, whole genome shotgun sequence.
ACCESSION   DADVXO010000010 DADVXO010000000
VERSION     DADVXO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.010"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            345..1454
                     /gene="pdhA"
                     /locus_tag="KB950_000470"
     CDS             345..1454
                     /gene="pdhA"
                     /locus_tag="KB950_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBB6856711.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1457..2434
                     /locus_tag="KB950_000471"
     CDS             1457..2434
                     /locus_tag="KB950_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBB6856712.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2459..4102
                     /locus_tag="KB950_000472"
     CDS             2459..4102
                     /locus_tag="KB950_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBB6856713.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4110..5516
                     /gene="lpdA"
                     /locus_tag="KB950_000473"
     CDS             4110..5516
                     /gene="lpdA"
                     /locus_tag="KB950_000473"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBB6856714.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5678..5953
                     /locus_tag="KB950_000474"
     CDS             5678..5953
                     /locus_tag="KB950_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBB6856715.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5959..6726
                     /locus_tag="KB950_000475"
     CDS             5959..6726
                     /locus_tag="KB950_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBB6856716.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6894..8723
                     /gene="typA"
                     /locus_tag="KB950_000476"
     CDS             6894..8723
                     /gene="typA"
                     /locus_tag="KB950_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBB6856717.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            8984..10756
                     /gene="recQ"
                     /locus_tag="KB950_000477"
     CDS             8984..10756
                     /gene="recQ"
                     /locus_tag="KB950_000477"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBB6856718.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10870..11172
                     /locus_tag="KB950_000478"
     CDS             10870..11172
                     /locus_tag="KB950_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBB6856719.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11175..12338
                     /locus_tag="KB950_000479"
     CDS             11175..12338
                     /locus_tag="KB950_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBB6856720.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12364..15792
                     /locus_tag="KB950_000480"
     CDS             12364..15792
                     /locus_tag="KB950_000480"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBB6856721.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15813..16937
                     /locus_tag="KB950_000481"
     CDS             15813..16937
                     /locus_tag="KB950_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856722.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16927..17364
                     /locus_tag="KB950_000482"
     CDS             16927..17364
                     /locus_tag="KB950_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6856723.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17348..17686
                     /locus_tag="KB950_000483"
     CDS             17348..17686
                     /locus_tag="KB950_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBB6856724.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17805..18365
                     /gene="rsmD"
                     /locus_tag="KB950_000484"
     CDS             17805..18365
                     /gene="rsmD"
                     /locus_tag="KB950_000484"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBB6856725.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18362..18853
                     /gene="coaD"
                     /locus_tag="KB950_000485"
     CDS             18362..18853
                     /gene="coaD"
                     /locus_tag="KB950_000485"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBB6856726.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18843..19886
                     /locus_tag="KB950_000486"
     CDS             18843..19886
                     /locus_tag="KB950_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBB6856727.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20045..20773
                     /locus_tag="KB950_000487"
     CDS             20045..20773
                     /locus_tag="KB950_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBB6856728.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20889..21134
                     /locus_tag="KB950_000488"
     CDS             20889..21134
                     /locus_tag="KB950_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6856729.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21131..21349
                     /locus_tag="KB950_000489"
     CDS             21131..21349
                     /locus_tag="KB950_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBB6856730.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21485..22024
                     /locus_tag="KB950_000490"
     CDS             21485..22024
                     /locus_tag="KB950_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBB6856731.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22017..24290
                     /locus_tag="KB950_000491"
     CDS             22017..24290
                     /locus_tag="KB950_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBB6856732.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24328..25374
                     /gene="holA"
                     /locus_tag="KB950_000492"
     CDS             24328..25374
                     /gene="holA"
                     /locus_tag="KB950_000492"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBB6856733.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25463..25717)
                     /locus_tag="KB950_000493"
     CDS             complement(25463..25717)
                     /locus_tag="KB950_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBB6856734.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26117..26386)
                     /locus_tag="KB950_000494"
     CDS             complement(26117..26386)
                     /locus_tag="KB950_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856735.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26856..27311)
                     /locus_tag="KB950_000495"
     CDS             complement(26856..27311)
                     /locus_tag="KB950_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBB6856736.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27449..29377)
                     /locus_tag="KB950_000496"
     CDS             complement(27449..29377)
                     /locus_tag="KB950_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBB6856737.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(29574..30407)
                     /locus_tag="KB950_000497"
     CDS             complement(29574..30407)
                     /locus_tag="KB950_000497"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBB6856738.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(30599..31303)
                     /locus_tag="KB950_000498"
     CDS             complement(30599..31303)
                     /locus_tag="KB950_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6856739.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(31326..31952)
                     /locus_tag="KB950_000499"
     CDS             complement(31326..31952)
                     /locus_tag="KB950_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6856740.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            32278..34653
                     /locus_tag="KB950_000500"
     CDS             32278..34653
                     /locus_tag="KB950_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBB6856741.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(34677..35168)
                     /locus_tag="KB950_000501"
     CDS             complement(34677..35168)
                     /locus_tag="KB950_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBB6856742.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            35355..36275
                     /locus_tag="KB950_000502"
     CDS             35355..36275
                     /locus_tag="KB950_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6856743.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            36429..36773
                     /locus_tag="KB950_000503"
     CDS             36429..36773
                     /locus_tag="KB950_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBB6856744.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(36963..37463)
                     /locus_tag="KB950_000504"
     CDS             complement(36963..37463)
                     /locus_tag="KB950_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6856745.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            37991..38074
                     /locus_tag="KB950_000505"
     tRNA            37991..38074
                     /locus_tag="KB950_000505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38025..38027,aa:Leu,seq:cag)
     gene            complement(38398..>38832)
                     /locus_tag="KB950_000506"
     CDS             complement(38398..>38832)
                     /locus_tag="KB950_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBB6856746.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        12859 a         6811 c         8046 g        11116 t
ORIGIN      
        1 acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta taacaccaac
       61 gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa atgcagatga
      121 aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata tgcggattgg
      181 cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg
      241 ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca
      301 gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag
      361 caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag
      421 cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg
      481 aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag
      541 cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc
      601 agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg
      661 tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac
      721 atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg
      781 gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa
      841 atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa
      901 tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg
      961 cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg
     1021 cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta tatactgtat
     1081 ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga
     1141 cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag
     1201 acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg
     1261 aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa
     1321 tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga
     1381 aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga
     1441 aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc
     1501 acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg
     1561 aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga
     1621 tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt
     1681 tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt
     1741 agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac ccatcacaat
     1801 tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg
     1861 tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa
     1921 aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa
     1981 actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa
     2041 agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg
     2101 tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga
     2161 tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg
     2221 tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc
     2281 tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc
     2341 tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat
     2401 cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat
     2461 ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa
     2521 atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa
     2581 tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa atgtgattgt
     2641 tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg caccaggaca
     2701 cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag ctaaaccggc
     2761 tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag tcttccaatt
     2821 taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa
     2881 accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg acaaatcagt
     2941 agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac
     3001 tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc
     3061 gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc
     3121 aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca
     3181 atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag
     3241 agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa
     3301 atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc
     3361 agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat
     3421 gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca
     3481 cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa
     3541 agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt
     3601 aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga
     3661 aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc acggactata
     3721 tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa
     3781 tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac
     3841 aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta
     3901 tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga
     3961 aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt
     4021 agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga
     4081 gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag
     4141 atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa
     4201 tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg
     4261 gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt
     4321 ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat
     4381 ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac
     4441 ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca
     4501 ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc
     4561 gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat
     4621 tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg
     4681 aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac
     4741 aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa
     4801 aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg
     4861 tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg
     4921 tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag
     4981 taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct
     5041 atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa
     5101 aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg
     5161 cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag
     5221 atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat
     5281 cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta
     5341 ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa
     5401 tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg
     5461 gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat atttgatttg
     5521 aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa
     5581 taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg
     5641 ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact
     5701 tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga
     5761 acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa
     5821 aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc
     5881 cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag
     5941 aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa
     6001 gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga
     6061 acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat
     6121 tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat
     6181 ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa
     6241 ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc
     6301 atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa
     6361 aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg
     6421 aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg
     6481 gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac
     6541 attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa
     6601 gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa
     6661 tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa
     6721 ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt
     6781 tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag
     6841 tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt
     6901 atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat
     6961 tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac
     7021 gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca
     7081 cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact
     7141 tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg
     7201 cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata
     7261 tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg
     7321 tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc
     7381 ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc
     7441 aagaaccaac catggcacct atttttgata caattattga aaaaatccct gctcctgtcg
     7501 ataacagcga cgaaccatta caattccaag tttcattact tgactacaac gattatgtag
     7561 gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat
     7621 taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg
     7681 gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta
     7741 tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta
     7801 tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg
     7861 ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac
     7921 ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt
     7981 ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg
     8041 aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac
     8101 cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct
     8161 tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt
     8221 tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga
     8281 cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc
     8341 aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt
     8401 attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat
     8461 atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga
     8521 aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa
     8581 aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg
     8641 aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa
     8701 aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata
     8761 aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa
     8821 tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt
     8881 ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt
     8941 cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa
     9001 acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat
     9061 tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca
     9121 gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat
     9181 gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc
     9241 gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt
     9301 actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg
     9361 gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt
     9421 ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat
     9481 tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa
     9541 aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt
     9601 aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca
     9661 atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa
     9721 aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga
     9781 aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg
     9841 tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct
     9901 agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat
     9961 cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag
    10021 agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta
    10081 tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg
    10141 caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca
    10201 gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa
    10261 agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac
    10321 ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat
    10381 atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag
    10441 agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca
    10501 gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga
    10561 tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga
    10621 aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca
    10681 aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa
    10741 cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc
    10801 tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg
    10861 taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca
    10921 gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa
    10981 gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct
    11041 gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga
    11101 gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc
    11161 aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct
    11221 tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt
    11281 atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt
    11341 gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa
    11401 accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt
    11461 tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc
    11521 aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa
    11581 gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta
    11641 tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt
    11701 tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag
    11761 gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa
    11821 agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt
    11881 tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg
    11941 gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag
    12001 cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt
    12061 tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa
    12121 aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat
    12181 ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt
    12241 taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca
    12301 tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt
    12361 tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct
    12421 tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga atattccgta
    12481 caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg
    12541 tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat
    12601 ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc
    12661 atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca
    12721 aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct
    12781 tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca
    12841 gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag
    12901 ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt
    12961 gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga
    13021 aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta
    13081 gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct
    13141 gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa
    13201 ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca
    13261 gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat
    13321 ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt
    13381 caatgccgaa tcactacaga agacccgttg aataactttt taccagacac agggaaaatc
    13441 gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg
    13501 ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct
    13561 acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga
    13621 gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga
    13681 aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga
    13741 gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt
    13801 atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca
    13861 agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc
    13921 gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat
    13981 caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact
    14041 ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg
    14101 gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg
    14161 cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat
    14221 cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt
    14281 cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg
    14341 gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt
    14401 cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt
    14461 gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt
    14521 ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc
    14581 agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac
    14641 gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat
    14701 gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac
    14761 cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct
    14821 gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag
    14881 gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc
    14941 aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg
    15001 gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca
    15061 ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc
    15121 ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt
    15181 ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa
    15241 attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt
    15301 ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca
    15361 tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg
    15421 cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc
    15481 ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg
    15541 gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct
    15601 ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg
    15661 atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc
    15721 gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg
    15781 actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga
    15841 ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg
    15901 aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg
    15961 agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg
    16021 attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg
    16081 aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg
    16141 ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc
    16201 aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag
    16261 aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata
    16321 acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt
    16381 atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc
    16441 cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa
    16501 aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa
    16561 cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg
    16621 aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga
    16681 cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag
    16741 aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt
    16801 ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca
    16861 ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg
    16921 atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct
    16981 gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa
    17041 gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa
    17101 aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga
    17161 aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca
    17221 cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa
    17281 atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt
    17341 aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt
    17401 atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt
    17461 ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga
    17521 agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat
    17581 cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga
    17641 aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag
    17701 cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt
    17761 tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag
    17821 gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag
    17881 ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat
    17941 tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc
    18001 atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa
    18061 ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac
    18121 ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac
    18181 aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag
    18241 ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc
    18301 aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc
    18361 aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac
    18421 ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc
    18481 aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca
    18541 tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga
    18601 gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg
    18661 agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc
    18721 aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg
    18781 agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc
    18841 aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca
    18901 ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc
    18961 tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta
    19021 cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt
    19081 ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc
    19141 aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac
    19201 ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa
    19261 catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa
    19321 gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa
    19381 tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt
    19441 taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat
    19501 cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt
    19561 tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg
    19621 caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa
    19681 aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt
    19741 cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg
    19801 ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc
    19861 ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt
    19921 tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa
    19981 aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt
    20041 ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat
    20101 agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat
    20161 agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga
    20221 aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc
    20281 atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat
    20341 caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga
    20401 tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc
    20461 tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga
    20521 acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc
    20581 caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc
    20641 agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga
    20701 agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt
    20761 tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga
    20821 tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag
    20881 gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc
    20941 aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag
    21001 ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc
    21061 tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa
    21121 tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat
    21181 cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga
    21241 atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat
    21301 acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga
    21361 cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa
    21421 agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata
    21481 aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa
    21541 gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc
    21601 tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat
    21661 cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg
    21721 ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca
    21781 gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg
    21841 tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga
    21901 ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca
    21961 agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt
    22021 ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga
    22081 ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt
    22141 ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca
    22201 taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat
    22261 ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg
    22321 aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag
    22381 cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg
    22441 agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca
    22501 ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa
    22561 atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg
    22621 aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag
    22681 gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat
    22741 cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat
    22801 ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg
    22861 caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca
    22921 aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt
    22981 tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt
    23041 ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc
    23101 tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag
    23161 gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt
    23221 tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg
    23281 gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac
    23341 gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt
    23401 tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga
    23461 ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca
    23521 gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt
    23581 tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata
    23641 tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg
    23701 acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa
    23761 tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc
    23821 aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg
    23881 atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc
    23941 tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg
    24001 aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc
    24061 acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga
    24121 ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac
    24181 tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct
    24241 ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag taattctcat
    24301 ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta
    24361 caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt
    24421 ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac
    24481 ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca
    24541 gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta
    24601 acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg
    24661 aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa
    24721 cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg
    24781 aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg
    24841 gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg
    24901 agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa
    24961 gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt
    25021 ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa
    25081 gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa
    25141 tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca
    25201 tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag
    25261 ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa
    25321 ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa
    25381 agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa
    25441 aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc
    25501 ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag
    25561 cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt
    25621 tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg
    25681 tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa
    25741 gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct
    25801 ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca
    25861 gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag
    25921 cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg
    25981 gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac
    26041 gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc
    26101 ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt
    26161 ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca
    26221 gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca
    26281 ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt
    26341 cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt
    26401 cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg
    26461 atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc
    26521 caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt
    26581 acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat
    26641 aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag
    26701 ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact
    26761 ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg
    26821 gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt
    26881 aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag
    26941 acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc
    27001 aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag
    27061 ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata
    27121 gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc
    27181 caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct
    27241 atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc
    27301 caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa
    27361 agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa
    27421 taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata
    27481 gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac
    27541 gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta
    27601 gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa
    27661 taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt
    27721 tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt
    27781 gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac
    27841 caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt
    27901 ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg
    27961 tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa
    28021 ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct
    28081 gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg
    28141 caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt
    28201 gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt
    28261 ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg
    28321 tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa
    28381 agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg
    28441 cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg
    28501 cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag
    28561 aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc caacctagga
    28621 aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat
    28681 tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta
    28741 attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg
    28801 gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca
    28861 cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat
    28921 ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa
    28981 catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat
    29041 tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc
    29101 ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac
    29161 caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt
    29221 tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg
    29281 gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc
    29341 cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa
    29401 acaaggatag cataataaaa atgtaattga taataataac agctaaatga gaacgataat
    29461 ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa
    29521 agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt
    29581 gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg
    29641 gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca
    29701 aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct
    29761 tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag
    29821 aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc
    29881 gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga
    29941 agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg
    30001 cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag
    30061 aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt
    30121 atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat
    30181 ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc
    30241 cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg
    30301 aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct
    30361 tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt
    30421 cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt
    30481 aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca
    30541 aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt
    30601 atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc
    30661 cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc
    30721 cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat
    30781 gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa
    30841 atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta
    30901 agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat
    30961 atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca tatccacctt
    31021 caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc
    31081 ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag
    31141 aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt
    31201 gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca
    31261 tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag
    31321 tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg
    31381 ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta
    31441 tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac
    31501 agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc
    31561 cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat
    31621 tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa
    31681 agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt
    31741 tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc
    31801 aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct
    31861 tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca
    31921 atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt
    31981 ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc
    32041 tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa
    32101 agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa
    32161 attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag
    32221 ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg
    32281 atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt
    32341 atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag
    32401 ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa
    32461 ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt
    32521 atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa
    32581 caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat
    32641 gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg
    32701 agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa
    32761 gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg
    32821 atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct
    32881 gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga
    32941 aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag
    33001 atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca
    33061 ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc
    33121 tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat
    33181 cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc
    33241 atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt
    33301 atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta
    33361 agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat
    33421 aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa
    33481 cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta
    33541 ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat
    33601 caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa
    33661 gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt
    33721 gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc
    33781 gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt
    33841 gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc
    33901 tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct
    33961 ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa
    34021 agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc
    34081 gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc
    34141 agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa
    34201 cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc
    34261 gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt
    34321 atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat
    34381 tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa
    34441 gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg
    34501 gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca
    34561 gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca
    34621 aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa
    34681 cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca
    34741 gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct
    34801 tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg
    34861 attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg
    34921 gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct
    34981 ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat
    35041 ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc
    35101 tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct
    35161 tttgccatag gaaccctcct gatatacata taaatattta ccatacatct atcttatcat
    35221 aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt
    35281 ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc
    35341 ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta
    35401 ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg
    35461 tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt
    35521 ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat
    35581 tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg
    35641 tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg
    35701 ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta
    35761 caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg
    35821 gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact
    35881 gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca
    35941 tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa
    36001 cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct
    36061 attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc
    36121 ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt
    36181 cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat
    36241 tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa
    36301 tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta
    36361 aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt
    36421 gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag
    36481 aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca
    36541 atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga
    36601 tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg
    36661 cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat
    36721 ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt
    36781 ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa
    36841 gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt
    36901 aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt
    36961 ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc
    37021 ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc
    37081 tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat
    37141 ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat
    37201 gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct
    37261 aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt
    37321 tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata
    37381 aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct
    37441 tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt
    37501 tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg
    37561 tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg
    37621 ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa
    37681 ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct
    37741 gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag
    37801 agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct
    37861 caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta
    37921 cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa
    37981 aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg
    38041 tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg
    38101 tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt
    38161 taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt
    38221 aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc
    38281 aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat
    38341 aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta
    38401 gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt
    38461 aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga
    38521 agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga
    38581 ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc
    38641 tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac
    38701 cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg
    38761 aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg
    38821 ttgtcctgag ta
//
LOCUS       DADVXO010000011        36844 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.011, whole genome shotgun sequence.
ACCESSION   DADVXO010000011 DADVXO010000000
VERSION     DADVXO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.011"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(70..798)
                     /locus_tag="KB950_000507"
     CDS             complement(70..798)
                     /locus_tag="KB950_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBB6856747.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            957..1838
                     /locus_tag="KB950_000508"
     CDS             957..1838
                     /locus_tag="KB950_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6856748.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(2075..3118)
                     /locus_tag="KB950_000509"
     CDS             complement(2075..3118)
                     /locus_tag="KB950_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6856749.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(3121..3789)
                     /locus_tag="KB950_000510"
     CDS             complement(3121..3789)
                     /locus_tag="KB950_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6856750.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            3931..6267
                     /locus_tag="KB950_000511"
     CDS             3931..6267
                     /locus_tag="KB950_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6856751.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(6382..6693)
                     /locus_tag="KB950_000512"
     CDS             complement(6382..6693)
                     /locus_tag="KB950_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856752.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            6874..8349
                     /locus_tag="KB950_000513"
     CDS             6874..8349
                     /locus_tag="KB950_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBB6856753.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8396..8980
                     /locus_tag="KB950_000514"
     CDS             8396..8980
                     /locus_tag="KB950_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBB6856754.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            8982..9365
                     /locus_tag="KB950_000515"
     CDS             8982..9365
                     /locus_tag="KB950_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBB6856755.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9682..9966)
                     /locus_tag="KB950_000516"
     CDS             complement(9682..9966)
                     /locus_tag="KB950_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBB6856756.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(10318..11028)
                     /locus_tag="KB950_000517"
     CDS             complement(10318..11028)
                     /locus_tag="KB950_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBB6856757.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(11025..12497)
                     /locus_tag="KB950_000518"
     CDS             complement(11025..12497)
                     /locus_tag="KB950_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6856758.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            12635..13108
                     /locus_tag="KB950_000519"
     CDS             12635..13108
                     /locus_tag="KB950_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6856759.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(13261..13812)
                     /locus_tag="KB950_000520"
     CDS             complement(13261..13812)
                     /locus_tag="KB950_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6856760.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            13917..14447
                     /locus_tag="KB950_000521"
     CDS             13917..14447
                     /locus_tag="KB950_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBB6856761.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(14930..15373)
                     /locus_tag="KB950_000522"
     CDS             complement(14930..15373)
                     /locus_tag="KB950_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6856762.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(15389..16549)
                     /locus_tag="KB950_000523"
     CDS             complement(15389..16549)
                     /locus_tag="KB950_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6856763.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17056..18756
                     /locus_tag="KB950_000524"
     CDS             17056..18756
                     /locus_tag="KB950_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856764.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(18858..19691)
                     /locus_tag="KB950_000525"
     CDS             complement(18858..19691)
                     /locus_tag="KB950_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBB6856765.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(19995..22160)
                     /locus_tag="KB950_000526"
     CDS             complement(19995..22160)
                     /locus_tag="KB950_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6856766.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            22420..24714
                     /locus_tag="KB950_000527"
     CDS             22420..24714
                     /locus_tag="KB950_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBB6856767.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            24707..25372
                     /gene="pgmB"
                     /locus_tag="KB950_000528"
     CDS             24707..25372
                     /gene="pgmB"
                     /locus_tag="KB950_000528"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBB6856768.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            25365..26372
                     /locus_tag="KB950_000529"
     CDS             25365..26372
                     /locus_tag="KB950_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6856769.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            26394..27413
                     /locus_tag="KB950_000530"
     CDS             26394..27413
                     /locus_tag="KB950_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6856770.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(27694..29061)
                     /gene="nhaC"
                     /locus_tag="KB950_000531"
     CDS             complement(27694..29061)
                     /gene="nhaC"
                     /locus_tag="KB950_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBB6856771.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(29170..30591)
                     /locus_tag="KB950_000532"
     CDS             complement(29170..30591)
                     /locus_tag="KB950_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6856772.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(30834..32711)
                     /gene="tdc"
                     /locus_tag="KB950_000533"
     CDS             complement(30834..32711)
                     /gene="tdc"
                     /locus_tag="KB950_000533"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBB6856773.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(33002..34258)
                     /locus_tag="KB950_000534"
     CDS             complement(33002..34258)
                     /locus_tag="KB950_000534"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBB6856774.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(34370..34575)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34838..36139
                     /locus_tag="KB950_000535"
     CDS             34838..36139
                     /locus_tag="KB950_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB6856775.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(36208..36807)
                     /locus_tag="KB950_000536"
     CDS             complement(36208..36807)
                     /locus_tag="KB950_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6856776.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        11353 a         7558 c         6619 g        11314 t
ORIGIN      
        1 atacaaaaaa actgactaaa agatttgatc ctttttagtc agcaaaaaat ggcttctctt
       61 tgatttttat tataacagtg gcataatcca atttgtccac atccagctca gtggcatgat
      121 aacgatcatt gcaggtatca tgtcagctac tgggaacatt ttcacgttga tcattctaaa
      181 accggtagcg accatcaaga atccgccgca agctttaaaa tctaagatca tgtctggtgt
      241 agtcaatgga taaataaatt ttgccaataa gaacaaaata taaaaaataa tgaattgggg
      301 aattgctatc acagaaacga cataccctaa attacacgca aaaatagctg ctgtgaaaaa
      361 atcaaggaca gatttagaaa tcaaaatcgt actgtctcct gtcatcccag aatctaaact
      421 tccatagatc cccgtaccac ttgcacaaaa tagaacaatg accgtcacta atgtgctgat
      481 aaattcatcg tgggtcatac caagcttttc actagggaag attttagaaa taggtacttg
      541 catcagcgca gcccccttat ttatcccatt acctaaatga acaatcaaac caatcgctgt
      601 tcctatcact aaagcaaaaa taacagccgg catatttttc atcggcgcaa tggaatagat
      661 tcccatcccc atcgaacaca caccaaaaac catcgtcagc tgtgctttaa aatgttcact
      721 taatttattt cctactaatc cgccaattag tccgccaaac aaaacggaac ctacattgat
      781 caaaactcca gttggcatca aaaaactcct ctctttttct acgaagcgaa aaaaatcaga
      841 aaaatttctg taattattgc tttcgctgca agaaagattg taacagacag agaagattac
      901 accaattcat tttttgtgtt aaagtattct ttattggaat aagtaggagg aatatgatgg
      961 atatccataa attcgaagtt tttcttgatt tagccgaaac aatgaatttc accaaaactg
     1021 ctgatcgaca attcaccacc caaggaaata tttctaaaca gattctttcg ttagaaacag
     1081 aattagacgt caaattattc gaacgcgccc atagaaaaat cgaattgacc gaagctggaa
     1141 gcttattgct cccatatgta aaaaatgtcg tagaacaata ccatgctatg caagaagtgc
     1201 ttgaaaccta tacgaaagac aacaatcttt cattaaatat cttaacgata ccgacaatga
     1261 tcaattataa aggtttttcc aaaatcaccg agtttttgaa acgccatcct gaatttacag
     1321 tccagttgaa agaagtggaa agtgtcgaat taacttacaa tgacgccaat gaacatagtg
     1381 acacgatcca ctttgctaga agtttccagg ccccaccaag ttcgattgag tggttgccta
     1441 ctgaaacaga cgattttgta gcagttctac ctaaaaatca tcctttagct agtaaaaaga
     1501 aattggatct aagcgaactg aaaaaggaga actttttgct ccttggcccc actacgaact
     1561 tgtatcaacc tgtactagat ctgtgtcgcg aagctggatt tgaaccaaaa atctcttata
     1621 aaggagcaag aatcgacttg atcatcaata tgattgcaaa tgacatgggg attgctattg
     1681 ttatggaaaa aacagtcaag aatctattaa aagaaaatac tgtgattctc cctatttctc
     1741 caacaacaga gagttatctt gcatttactc gtaaagtcgg tgagcattct ttggcttcag
     1801 atacattttg ggagtattta aatcaagaag aaaactaagc cctacatttt ctgtccatct
     1861 atccacccaa aaaaagagag cgacgcatga ctttcgctct ctttcttgca tcatgtcctt
     1921 gtttcaatga cacctctaac atttctcgta gttcaataag aaagcagcga tttttatgcc
     1981 aaaagaaccg taagaacatt tccctaatca caaaaaaacg actaaaaacc cctaaaaacc
     2041 ttcaatagaa tttttagggg ttttattctt tttcctattg tttacttaac cgtatcattg
     2101 ctgtcgttcc tactccttcg atactgctat aatcaatacg tccatcatgc agttctagaa
     2161 tttttttaac cagtgaaagt cctagcccat tcccagaaat ttgacgactt gtatctcctt
     2221 gataaaattt ttcaaacatt cgatctctgg tctcttcatt cattcctaca cctgaatcgg
     2281 tcacttccag tacaatctct gtggctgtct caaatagttt gatatgaatc tgtccattga
     2341 tttgattgta ctttatggcg ttatccatga tattcagcca cacttggtac aaaaactctt
     2401 cattgcctgt gtaatttact gcagcaagtt cgatatccaa ttctagctgt tctttctccc
     2461 atttaggctg aaaaaaaacg attacttctc tgatttgttc atctaatcga tactctttct
     2521 tttccagttg gatacgctgg ttttccagtt tgttcaattt taagatgttc tctgttaatt
     2581 gtgaaagctg cgtgatactt tcgataatcc gatataaaaa tatttggcgt tcttcgtcag
     2641 agagatttgg ggcttgtaac aattgaacat atccttggat tgttgctagt ggtgttttga
     2701 attcatggga tacactggac acaaagtcat tcctcagtgt ctcgatacta ttgagttctt
     2761 ggaccataac gttgaaatct ttatacagtt gctgtacttc tgctactttc tgctgttcat
     2821 ccattcgaat cgaaaaatct cccgttgcta ccagactcat atttgtccct agcgcactga
     2881 ttggatgcag gatacgccgc ccaacaaaaa tcgatattcc tgtacccacg accatactga
     2941 accccgcaaa gacgaataac gggtataacg ttggttttcc agccgattca gcaggcgaga
     3001 tagaatgtcg agacaataag aaaataaaac cgagaaaaca cagcagtgtc actaagatcg
     3061 aaatgaagac aatactagca aaatagatcc ataattgtga aaatagcttt tttttcatct
     3121 ttaaataacc gccttatagc ctagtccccg tactgtgatg atttgaaaat cttccgtctt
     3181 ttcaaaacgg gtccttaaac gtttgatatg tacatcgatc gttcgttctt ctgtattcgt
     3241 atctaatccc caaatatcat ccatcaactg ttggcgagtg aagatcttgt tgggataaga
     3301 cagtaattta tacagaagca gaaattcttt ttgaggcaga atcaacgaat gctgatcttg
     3361 taacactgta taagcatcta gctctaattt tgtttgtccg attatcaagc tactgctgta
     3421 attgatcttt gaacgtcgaa gcaatgcttc cacacgtagg atcatctcat tgacatcgat
     3481 gggttttgtc atataatcat ctgctcctaa tttgaacccg cgtttcttat cctcaaaatc
     3541 ggcctttgcc gtaatcatta gaacaggtgt gtcaaatctg gtgctacgta atgagtctag
     3601 caagtcatag ccatccattt tcggcatcat gatatctgta atgattaatt ctatacgtgt
     3661 cgcttctagt tttttcagcg catcctctcc attcaatgcg gtgatgactg aaaagcccgc
     3721 tcgttccaac acagaacggt aaagcaactg taattgcgga tcatcttcta ctactaaaat
     3781 acgattcatt gatctccctc tgttttttga aaaatttcta gattttttct tgtagttcat
     3841 cttcagttaa catttatttc gtatactacg atcattgttc tgatatactt attagtatac
     3901 cgaatatttc aatacgggag aagggtgatt atgcttcaat taaaagatat aaaaaaatat
     3961 tataaagtcg gcgaaaccac caccaaagca ttggatggtg tgtctgtcgc tttcagacaa
     4021 aaagaattcg tagcgatttt aggtcctagt ggttctggta aaacgactat gctgaatgtg
     4081 attggtggac tagataatta tgattccggt gatatggtaa tcaacggaaa gtctactaaa
     4141 gactttaagg acagtgactg ggatgcctac cgaaacaact ctatcggttt catttttcaa
     4201 agttacaact tgattggtca tctaggcatt attgaaaacg ttgaacttgg gatgacatta
     4261 agtggtgttt ctaaagacga aaaacgaaaa cgggcagaag atgcattgca ccgagtcggc
     4321 ttgacaaatc atatgcataa aaagccaaat cagttatctg gtggacaaat gcagcgagtg
     4381 gccatcgctc gagcactagc caatgaccca gatatccttt tgtgcgatga accaaccgga
     4441 gcgttagata cagaaacaag tatccagatc atggaattga tccaagagtt atcgaaagaa
     4501 aaattagtga tcatggtcac acataatcct gaactggccc atcaatacgc agatcgaatc
     4561 attgaatttt ctgatggtaa gatcttaaca gattcccatc ctcacatcga acgaccaaaa
     4621 gatgatcaat ttaacttacg cagaacaaag atgagttttt ggacagcgtt aaagctttcc
     4681 ttcaacaata tccgaacaaa aaaaggacgt actttcttga cctcctttgc ttccagtatt
     4741 gggatcatcg gaatcgcgat cgttctgtcc ctttcttctg gattccaaaa acaaatcgat
     4801 aacacgcaag ctgaaacgat ggcgaaattt ccgattacta tttctaaggt aacgactaac
     4861 cagactcgtg acgatgccgg attgggtgca agcaaagcgg attatccaga cagcaaaacg
     4921 attactgcaa aagtcagcga agaagataaa gcacagcaca cgaataagat tgatcaaaca
     4981 tacgtcgatt acgtaacaga catcgatcct aacttaagta ataatatcgg atttacccgt
     5041 actacaggaa tcaatctttt acgtgatgta aatggcaaag tacagcctgt cagcttctcg
     5101 aatcaaaatc ctgatacgga atctctttct ttatctagta cgatgtcagc aatgacaggt
     5161 gtgggagtgt caagtttccc tacacagctt gacactagca aagaaaactt tttaaaagac
     5221 aattattctt tacttgccgg aagttatcca gcctcagcaa cagatgtcgt gctgatcgta
     5281 gacggcaata ataatactaa tatcaacgca ttgaagaatc ttggatttga tgtgaaagaa
     5341 gacgaaaaac ttgattttga tgatatcgtt ggtacaacat tcaaattagt aaacaacaat
     5401 acttactata caaaacttcc aactgggaat tttatcccta atacagatta tgatgcgatg
     5461 tatcagaatg cttctgatga attgaagatc tcggggatcc tacgcgtgaa atcttcttct
     5521 acaatgaatc tcttatctcc aggaatcgct tatagcgatc aattaacgac tcaaatcgtc
     5581 aacgaaaaca aagaatcaga aatcgtcaaa gctcaaagag acagtggtgt aaatgtctta
     5641 actactgaaa aagttgacga aaatgccaaa caaacattgc ttagttattt aggtggagac
     5701 agcttgcctt caagtatcat gatttatccg aacaattttg aagacaaaga aaagatcttg
     5761 gattatctag atgattacaa taaaggcaaa tcagatgaag ataaaatcat ttatactgat
     5821 ttagctggaa cgatgactga actgactggc ggattgatgg atgctatcac ttatgtcctt
     5881 attgcttttg cagggatctc tttagtaaca agtatgatca tgatcagtat cattacttat
     5941 acatctgtga ttgaacggac aaaagaaatc ggtgtactaa aagctttagg tgcacgtaaa
     6001 aaagacatca ctcgcgtatt tgacgcagaa acgtgtattt taggtatttc ttctgggata
     6061 ttaggtgtgt ttattgcatg gctagctacg ttccctatca atagcatctt atacaacatg
     6121 acggatctaa aaaatgttgc tcaactaaat cctgtccatg cgattattct agtgatcgtc
     6181 tctactatat tgacgatgct tggcggtcat cttcctgcac gaatggcagc gaaaaaagac
     6241 gccgctatcg ctttgcgtgc agaataaggt tgtgacaacc acatttagat ttgagtcata
     6301 agagcgctga aatagtagaa aacagcaaga aacgaggaag taatatgttc cttgtttcgc
     6361 gctgtttttt ttaattattt tttataattc tggtatactt ttctttcctt tgtaccacaa
     6421 ctcttctggc gtatacctgt cttttccatt tatgaatact gttccatctg cttcaatcgt
     6481 tacttcagtc ccaatccaca tattacgaga aagtgcgaga agattttttc tttttcctct
     6541 tccttttttt ccaaattgca aggcatcggt tatctcttct gtccaatgag atttcacgta
     6601 ttttacttct ccgttttcaa atgtaacttt caaaatattc gctatatttt ccacttttac
     6661 tgctttagga aatgactcct ccattttttt cactttcctt cctccaacac taagtatgcg
     6721 ctttctttta gtgataagta tatcatagtt tattctatta tcaaaaaaac gcatccattc
     6781 cattgtagaa tactttaccg aacaagttga attgttttcc tctttttcac cttttactat
     6841 agataggtaa agacaactaa ggaggaatct ctcatgacag atcaaccgta tgatttaaga
     6901 aaagtcatcg aagaactaaa aacacttcca ggtcaatatc acgaaacaga tattgaaatc
     6961 gatcctgaag cagaattatc tggtgtttac cgttatatcg gtgctggcgg tacagtaaaa
     7021 cgtccgactc aagaaggacc agctatgact ttcaacaata tcaaaggttt ccccggcaca
     7081 cgtgtgaaca tcggaacaat ggctagtcgt aaacgtgtag gtgaaatttt gcaccatgat
     7141 tatcgaacac ttggtcgttt cttgaaagac gctgtagaaa atccaatcaa acctgtgaaa
     7201 gtagacaaag cagacgctcc agcacaagaa gtcgttcatc tagctaccga tgcagacttc
     7261 gatattcgca acattttgcc tgcacctaca aatactcctg aagatgctgg tccatacatc
     7321 acaatgggtg tagtcttagg atcagatcca gaaaaaacaa tgagcgatgt aacgatccat
     7381 cgtatggttc tagaagataa agatacgatc ggtatgtaca tcatgcctgg cggacgccac
     7441 attggtcact tccaaaaaca attcgaagcc ttgaataaac caatgcctat cactatcaat
     7501 attggtttag atccagcaat tgccattggc acgactttcg aaccacctac cactccactt
     7561 ggctacaatg aactatggtg tgcaggagct ttgcgtaacg aacctattca aatcgttgat
     7621 ggtatagcag tcgatgaagt gggtattgct cgtgctgaat tcatcattga agcagaaatc
     7681 atgccaaatg aaacaatgca agaagatatc aacacgaact caggaaaagc aatgccagaa
     7741 tttcctggct atactgggga tgctaatcca gctgtacaag tagtgaaagt caaagcagtt
     7801 actcaccgaa aagacaatcc aatcatgcaa acaacgattg gtcccagtga agaacacgta
     7861 tctatggccg gtattcctac agaagcaagt attcttgcct tggtcgataa agctattcca
     7921 ggaaaagtag taaatgtcta caatcctcct gcaggtggtg gtaaattgat gaccatcatg
     7981 caaatccgta aagaaaacga agcggatgaa ggtattcagc gtcaagcagc tattcttgct
     8041 ttatcagcct tcaaagaatt gaagacagtc attttagtcg atgaagatgt tgatattttc
     8101 gacatgaacg atgtagtttg gactttcaac actcgtttcc aaggagataa agatatcatc
     8161 gtgatcccag gaatgaggaa ccatccattg gacccatcag aattaccaga atataatcct
     8221 ggctctattc gtttccgtgg gataagtgca aaaactatct ttgacggtac agttcctttc
     8281 gatatgaaag aacattttgt acgtgcaaaa ttccaagaag tagaagattg ggaaaaatat
     8341 ttgaaataat gaatgatctg caactgtttt gacatcataa aaaggagtgc ggaaaatgga
     8401 acaattggca caacggaaga aaaaaatcgt gattggtgta agtggcgctt caggcaccat
     8461 ttatgccatc aacctagtga agaaactaaa agaatatccg atgatagaaa cacacggaat
     8521 catcagtcca tgggcaaaac agaacttaaa gctggaatcc gacttatcac taactgaatt
     8581 cgaaagcctg tttgattacc attattcaaa caaagattta ggtgctgctg tcgccagcgg
     8641 atcatttttg accgatggaa tgatcatcgt accggcaagt atgaaaacag tcgcaggtat
     8701 ctctgttggt ttaggcgaca acctgattgc acgtgctgct gatgtaacaa tgaaggagca
     8761 acgaaagtta gtcattgttc ctcgtgaaac gccattaaat gccattcact tggaaaactt
     8821 gactaaatta gcacgactag gtgtgcaaat cataccacca attcctgctt tttataatca
     8881 gcctcaaacg atccaagatc ttgttaatca ccatacaatg aaacttttgg atcaatttga
     8941 aattgtaaat gagcattcaa aacgttggca gggtgattaa tatggaaaaa gaaacgatag
     9001 ttgaagtaac caaactaggt ttcacgatcc aagctgctgt taaagtaatc ggtaaagact
     9061 tattgatcac tttaacaggc ggagatacac cacatatcgg aacagtcact acattttctc
     9121 gtgatacaga gatgcaaact gtacgtttcc ctagccacga tggtcggttc cataaagacg
     9181 atgtcttagc cgaaaaaata gctgtgatca tcaaaactgc cgttccggga agttgtacga
     9241 tcacttccgg tatccatgtt gaccatatct ccaatgcaca aatcaacgcc tcttttccga
     9301 tgtcagaaga gttaggttac aagattttag attggttgga aaaaatagat ttccatatca
     9361 aataaccagt ttattataaa gatggtgaaa aaccattatg aaagaagagg ttgtgaaatc
     9421 agcgttatga cctgagtatg agagaaaaaa atggaaaaat agcttctcat attttgccgt
     9481 ttttgagcta ataccgaggg acagctgatt gaacgccgtt tatcaagagg ttgtgataaa
     9541 gccgcttcgt ttcgagtagt aagaaaaatt ttgtaaacat gggtttccac atttttgcat
     9601 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcaaga ggttgtgaca
     9661 cgtcttttgt cacaacctct tttatttttg cttcactgta actgctacaa tatcaccgat
     9721 ttcttttccg atgtttttac gaatatcttt tcgtagtcca aggatgtagc agactgaacc
     9781 atctggattt ttcacgccca tattcacgat actgccttca tacggcacat tgtcaaaagt
     9841 tgcttggact ttaactcttc cagcaccgaa ttcttttctt atatcatacg gaaaaataat
     9901 atatgccccg cccttgccga cttcggaagc atagattttt gattcaaaag aataaatttc
     9961 cctcatttta tttctcctat tttttacatc tagtatagca tagcaaaaaa aagaaggaac
    10021 tgtgacaaaa gtcttgtcgc agttcctttt ttcgaataaa cggtgttctc ctgccaactc
    10081 ctcggtcaca agtcacaata gtcaacaaac atgagaagct gttttttgaa attgctcctt
    10141 gtgactcaga gttaaacggc aggctcacaa cctcttcctt aaacggtgtt caaaagcttg
    10201 catctacggc actaagccca atcagatgaa aaatatggag tgctattttt atctgattgt
    10261 tcttactgct tgatgcagag cagctttttc acaacctcct acatggtgtt caaaatatta
    10321 ttcgcctttt gcttctttca ctaattttgc gaatgcttgt tgttggaatt cagcagctaa
    10381 ggctttcgta cgttgttctt gctctgcttt gctcattttt ggattttcag tcattgcttt
    10441 taccataccg tcttgaacat aagcttttgc ttcactttcc atcgtacttt gctgtttctt
    10501 cactaattca gctaacttgg ctgcttggtc cgtacttaat tctacccatg tctctgggat
    10561 tttgaatgta ttcactcggc tgtcatattc gtgattgtta tcagcaatat tgaaccagaa
    10621 tttatacatg tcgtttttgt attcccaata agtagttttc gtcacttttt cagcatcgcc
    10681 gtctgtcttt tctactttat ttgtttcgtt atccgtacca gttgcttttg gtttttcttg
    10741 tttttcattt gttcggtaaa gatacacttt ttcttttcca ttccctaaag ctttatacaa
    10801 aagcatatct gctccttggg aatcagcact ggaaacaatt ttttctgttt ttgtttccgt
    10861 tactttttcc attccaaagt gattggacaa attagcgacg atcaaactta gtgaagcgac
    10921 gaagattagt ccgaaaatca aagataaaaa ggtttgccat gtttttttag cgaacacgtt
    10981 cgttaaagca aaagcaagca agctgacaat caaaactcca ataatcatgc attatccccc
    11041 gctttcatta gaacaggttc ttttttcttc aataaaaagg caataataaa gccaagaatc
    11101 ccaaagatga tcgctacgat aaacgcggca tggtaacctg acagtgtggc attgattgcc
    11161 tgatctttat aagctaatgg cgtattatcc aatacagatt tgcttgggat atttgctttt
    11221 gtcacattcg tcaacacact gatcaaaata gctgtaccaa tcgagcttgc gatttgacgg
    11281 aatgtattat ttaccgctgt cccatgactg atcaagttat ttggcaatgc attcatacca
    11341 gaagtcgtta ccggcatcat gaccatggag atcccaaaca tacggatcgc ataaaggatc
    11401 acgatatata aaactggagt cgtttttgtc aagaaagcaa atggtgcagt tgccgctgtc
    11461 aaaatgaaca tccctgtgat ggctaaacgt ttcgcgccgt atttatcgaa aatcgcaccc
    11521 gtcacaggca tcatagcccc catgatcaat gcacccggca acaacatcaa gccagaatgg
    11581 aatgctgatt ctccacgaat attttgaata tacaatggta cgaccatttc tgcccctacc
    11641 atcgccatat ttgttacacc agctaaaaca gctgcaatcg tgaatgtaga tgatttgaat
    11701 acacgaagtt ccaagaatgg atgttccaaa tgtaattgac gccatgagaa caaggcgatg
    11761 aaaatcacac caacaactaa gaaaccaatt actttcaagc ttccccagcc gtcatctcct
    11821 acactagaaa atccgtaaag taaactaccg aatccaatcg ttgataaaat agcggataaa
    11881 atatcaatgc taggatttga caatggaata acacttttca ttaggaagaa tgctaaaacc
    11941 aagacaagaa caacgattgg cagtaccata ccaaacaagt cacgccaatg gtagttatca
    12001 atgatataac cagataatgt agggcctaaa gcaggagcaa gtccaataac gatccctgac
    12061 atccccattg ctgatcctct tttttctggc gggaaaatag atagcatgat attttgcaat
    12121 aatggcatcg atactcccac accgcaagct tggatcaaac gaccgattag taatgtacta
    12181 aaattaggtg ctgtaaagca agtaatcgtt ccgatcaaaa agatcgtcat ggcagaaaga
    12241 tataaatttc ttgaactgaa tttattgatc agccatgctg ttattgggat catgatcccg
    12301 ttgactagca agaaaccagt cgttaaccat tgcacatcag acgctgaaat atcgaaagct
    12361 ttcatcaatg taggaaaagc tgtcgtcaat aaggtctgat tcaaaacggt acaaaatgta
    12421 ccaattaata agacaacaac taaaagtgtc cgattatatg gttttccgta aatatctacg
    12481 ggttggttgt tactcatatt tctccttctt tctgaatttt ttctgaaact actagaatac
    12541 tttagtccta aattcgctat ttgtcaacct acttgacaca taagttaata cggatataat
    12601 taagggaagc aagaggacaa aaggagtgac aaaaatggtt ggggcttccc ttaaacgttt
    12661 gttggaaaat gatcaattgg ttgttggcat cagtgaatta agtgatatcg taggtgtgtc
    12721 tccacgtcaa ttaaggtatt gggaacaaaa gggattcatt cgttcgatcg aagctgatgc
    12781 gaattgtcct agaaaatatc gtttacctac agtcataaaa gtagaactga tcaaaaaata
    12841 tttagatgac ggatttactt tgtcaaaagc tgcagaaaag gcagaaatcc gccaaaagaa
    12901 aatgcaccat gtccgtaaag ttttttcaaa agcaatcaaa gatatcgaat tgatcgacga
    12961 acgctatacg attttacaaa tcggtaactt caaagaagag aatgctaaga tgtacatcat
    13021 tcatgacaac gagacagacg aattaactta caagctcctt cctgtcgatg aaaaagctga
    13081 ctacgaacag ttgttaaaag aactctaaac gccactaatc tcctaagtac caagcttttt
    13141 aataagtaga tgctacataa atacttgaaa aaaggcctgt ttgaacataa gaatatccga
    13201 acggtgatct gcgtttcttt catatagaaa ggaattctcc atcactgttc ggatattttt
    13261 ttattttttt cggttacttt cttctaatac atctgttagc caatttgcat attctgtttc
    13321 ccgattgatc gcatgcgcta aaatcaggta gtgtccgtaa ttttcatgtt tttctttttc
    13381 agtaggaaag atcgtttttt ttcgctctaa tagatgaagt cgtttttctt catgaaggtc
    13441 acgctgctgc tgaataattt ctactaatgc cggatcattc acgtctttga cgaaatacaa
    13501 tttcagaaca aattcgtctc gattaaccgg tagttcattg gtgggtgtat gaatccactc
    13561 tgccaattgt tccttccctt cttctgttaa ttcataaact ttcttttcca atttttcacc
    13621 tgtgatctct acttgatgct gaatgattcc ttgctcttcc atttttgcaa gttcaggata
    13681 aatctgacta tgttttgctt gccaaaattc tcctatttca tttttgaacg attgattcaa
    13741 ttcatatcct gtcatcttct tcttacttaa taaccctaac aaaatatatc gaagtgtatt
    13801 ttttcttgcc atcagttttc cctccttgac aaatctttaa atccattcta tcataaatga
    13861 gtaatcgaaa acatgtagtt tattacatgt tttaatttat aaggagatgc ttaaacatga
    13921 aagaatttaa agaattggaa gattttattg gaactcactt tatctatacg tatgacaatg
    13981 gttgggaata tgaatggtat gccaaaaatg accatactgt tgattaccgt atccatggcg
    14041 gaatggtcaa aggccgttgg gtaaaagacc aagaagctga tatcgttaaa ttgacggacg
    14101 gcgtattcaa aatcacttgg acagagccaa cagggacaga cgtggcattg gactttatgc
    14161 caaatgaaaa gaaacttcac ggaatgatct ttttccctaa atgggttgaa gaacatccag
    14221 aaatcactgt tactttccaa aacgaacata tcgcagaaat ggaagcagct cgtgaaaaat
    14281 atgaaactta tccaaaatta cttgttcctg aatttgctac gatcacttat atgggtgatg
    14341 caggtataga caatgaagat gttattagcg aagaaccata cgatggaatg ccagatgata
    14401 tccgtagcgg aaaatacttc gatgaaaatt ataaacgagt aaaataaggt tgtgaaagag
    14461 aggttgtgaa atcagcgttc tgacctgagt attagagaaa gaaacggaaa aatagttcct
    14521 catgttttgc cgttttttga gctaataccg agggtcagct gattgaacac cgtttatcaa
    14581 gaggttgtga aatcagcgtt atgctttgag taataagaac gctagttaga aaatagcttt
    14641 tcatattttc taggctagtg ggcttattac cgagaagcag ccgtttgaac accgtttatc
    14701 aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaatgg aaaaatagtt
    14761 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat
    14821 caaagattgt aacaactgca tattcacaca gttaaattag tgacttcaaa atgaaataat
    14881 gctcccagca aaaaattagg ctgggagcat tatttcgtta ttctgttcat caaaaatcag
    14941 cgttttgatt ccatttcatc acaagttcct cctcatttac tgcttcatct ttttgctgtg
    15001 tttgaattgt aaaaccttgt gaaagataga aatgaatagc tgattgattt ttttgataga
    15061 ctgtcaaagt cagtgtttta ttggtttctt ttattttttt cattagttgc gtaccgattc
    15121 tttgttgccg gtattccctt ttcacaaaga ttcctgcgat aaactgatca atcaccccag
    15181 caaaaccaat aatttcttga tctatttctg ctaagtaaat cactgcatct ggtaaagctt
    15241 tcctaacaga ttctacttga ttctcccaat aagttgatgg aatgaattca tgagaatcca
    15301 agttagcatg cagccaaatc gatagaacag attccagttc tttggcagac aattttgttg
    15361 ttgtgcggat cattttttct ctccttactt attcattgat tttaaaataa aaaccatttt
    15421 tttgaagctc ttttacaagt tttcgcccct caaaaaagta tatcttctct tgttttactt
    15481 ttctaatacc aaaagaaaca gcaactttag gatcatcagc tgaaacgatt cgataagttc
    15541 caccaggcaa ttgatccagc ttgactttcc catgatggtc gctttgtact gaaaacggca
    15601 caccatttct agtaagtgtt tttttcttat gtttcaaaat gaatgaacta ccatttaccg
    15661 gtttccctga cgaatcacta ataataaatg aaaaatcaag cactttttta cttaatctat
    15721 actgatagat tagtcgtccg ataaacacta acagtatgac tacagctatt atcgttcctg
    15781 caagaataaa ccattgccgc atggttcttt cctgatttcc ttttccttct tcttttttta
    15841 ccgattcgtt ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg ttgatcatat
    15901 acggcgtaca tgtaagcaag gtaactaagt cttctccttc tgaaatcgtt agaacagacg
    15961 tgtcatctgg cagtaccact tgtatatttt taacttgata agccaatttc tcccccaaaa
    16021 cagtcaaaat aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat aaccgtcgat
    16081 taggtaaacc gctgtgagca gagatcactg aatgcgtcga atttccacca agcgggaaag
    16141 aggttccttg caagacgcct gctccatttt caaggatcct accattgagc cgatcataaa
    16201 gtgaaatatt taattggatc ttaggtatat tgatacttcc gatcagccat tcctctaatt
    16261 ggctggatgt tgtactttca tttctgttct tttcattgaa aggatcagta ccaggattga
    16321 atccttggtc agttaatcgt tggtttttct cctccatttt ctttcgctgc acttcaattt
    16381 cagctgcggt ccgtttttgt acttgttcga tacgtttttg atcaataaaa ctatttaaag
    16441 aatccacata aaatggatat aaagcgatca gtaaccctac gacgatacat attaataaaa
    16501 acaaacgaaa caaaagtcga cgagactgtt tttgcttact ctgcttcaca tgtgtcctcc
    16561 cttgcaagtt tttttgttct ttcttttatt atatgggttc aggaaaaaaa tagaaaatga
    16621 aagccgatga ggttgtgaaa aagctgtcct gcatcaagta ataagtacag cccaaaaaaa
    16681 tagctactca tattttttgt tgggttgggc ttattaccgc agatgcaagc ttttgaacac
    16741 cgtttaagga agaggttgtg aaaactgcgt tatgctttga gtaataagag cgctagtcag
    16801 aaaatagctt ctcatatttt ctaggctagt gagcttatta ccgagaagca gctgattgaa
    16861 caccgtttac cgagaggtca tgaaaaagct actttgtttc gagtattaag aaaaattctg
    16921 tttcaaaaca aggagatgca acaaaactta tgtcgcatcc tctctcctta gaactttcac
    16981 ttatgttttt cttgtaaaat tcataaatat tttcatgaat tttctaattt cttgcctacc
    17041 tctcgcatag gatatatgat gaatttatct aaagtttgca aaggagttcg acaattgaaa
    17101 aaatatggcc catttttagc aatttttttc attgctttgg catttctcat gcctcattta
    17161 ttcacacata aactaatttt gggtgctgat gccccattcc attacaatcg gttctatgat
    17221 gccgcgcaac aaattaaata tggggatttc cattattttc cttcccttta tggattccag
    17281 caaagtggca ggatcgtgaa tgccgtttac ggcccatttt tcgcttattt acaaggattg
    17341 atcgtgttgc ttgcaggtag ctggtttcat tatcagattc tttcaaattt cattctttat
    17401 cttatttcag gattttctat gctgatttta ctgagatatg tcaatgttcg aaattgggta
    17461 agtgtaccag tagccgtttt attcatgagt acgtattcta ttcaatactg gtctatcaac
    17521 caaggattca ctagctgggg gacttgtttt tatcctttat gtatgatccc tattgctgat
    17581 ttcgtccttc ataaaaagtt ccctgttatc aaagtcgctg tctcagtggg acttatgaca
    17641 cagatccatt tactgtcttc gatcatgtta gctttgatgt atatcccttt ttatctttat
    17701 tactttttta accagactga aaaaggaaaa gcactgcttc aactgattaa gtcggtcttg
    17761 ctttattttg ttctgacagc aaatatttgg gcaggattag ctattgttta cagtgggaat
    17821 aagattcttc ctccctttgt taacagagaa atgagtcaaa aggcgataga tctggatggc
    17881 agttattggc tccattaccc taaggccttt cctatattga tattatcagg ttttttgctt
    17941 tttattttct ttcaacaaaa gaacacctta ttccaaaaaa tcgtgttgta cacaagtctt
    18001 gttttctttt ttctttctac tagcttgatt ccatggacga cattgatcga aaaaaatgtg
    18061 ccttttatta gtttgatcca gtttccattt cgatttttcg tgccatttac gctattgctt
    18121 ctactttacc ttgccctttc actgaaccag tggtctgaac aaaagtggtt ccgtctttta
    18181 agtatatttg gcattcttat tttttcagca cagacgatcc agaacttata ccagcatctg
    18241 gaaaagtggg aaaatgaaac atttgtcagt cgacatactt atctattcga tacgccggaa
    18301 gaagcaagga aaacattctt cagcaaagat atgtctacca gcttgttcgt tttccaaaaa
    18361 accacacctg actatctgcc aatctataag gagacaaaag acaataaata cgaccggtat
    18421 aacgaagaaa tactagaaaa cgaaacaact tttataaaaa cacatccagg cggtcttttg
    18481 acaatcaagt ggaaaaacac tggggacaaa actgttcata tccctgttat cgtctacgat
    18541 cggactgtgc tacagcaaaa tggaaaaaca ttgactgatt atgaagtaac agatatcggg
    18601 accccaatcg tgaagcagca aaaaggaatc aacgagctca cgcttcatta tcaaacgcca
    18661 atctatttct atttcattct ttctcttact ttgattggct ggtttacctt gctatgttta
    18721 tttatttatc atagatacaa gcttttacgt gcttaggcaa aagggataaa tacatccgat
    18781 caaaaaactc tccaaacggt ataatcgttt ggagagtttt attttaatca gtttttttat
    18841 gttatatgtt atatttttta ttcgttcaca tctaattcga tttccacccc gtagtgatca
    18901 ctgatgattg gactatcgat cccatcaaaa atccgttgat attttttaac ctgcattcct
    18961 tcagggacga aaataaaatc tatacgcaat ttttctgtat tttgttccca accatctatt
    19021 ttcttttcca cagtagctgc ttttcctttt tcattcgcga caacatacga atcttgaaga
    19081 agaaggcaac tttctcttac agtataataa ccttgagagt ctactggatt attgaaatca
    19141 cctaggaaaa aaagtggtac gcgagtttcc aaaaagtagt tttccaagtt tttccactca
    19201 aaataaaagc cacttttttt atctgtccac caagaaaagt ggcagctcga gacaaaaatg
    19261 tcctgatcat ctatctttgt tttcccaatc aagatcttgc gtgtctgata atcttctttc
    19321 ttttgaaatt gggagacggt aaggacctga ctttccaatg tttttttcgt aagcagtgca
    19381 tttccttctt catatttatc ataaccgatg tggctcattt cccaactcca aaaatactct
    19441 tttccgtgtt cctttaaaag ctgaactaaa cggtaggcaa aattatcttt acggataggt
    19501 acttgattct ttaccggaca gaacttcatt agcttcgcag gttctacttc ttcagattcc
    19561 agtaattggt tgatttcttg taaagcaatc acttcataat cttctaacag gatcttatct
    19621 gctaattctt gcaatttttt ctctggttct ggctcaagcc agctatgcgt gttgagtgtc
    19681 atgaatttca ttggttttct ccttgtcttc atttcgaaac aaaagggttg tgacaaaatt
    19741 tttgtcacaa cctctccagc aaatagtgtt ctcctgctaa ctcctcagta ataagctcaa
    19801 tggaccagga gctatttttt gtttggctgt ttttatcact tggtgcaggg cagctttttc
    19861 acgacctctc cactaaacag tattcaaact gctgcttctc ggtaataagc ccaaccaaac
    19921 gaaaaatagg aggagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt
    19981 ttcacaacct ctccttacat tttaatggag ccgatcactt taccagctgt cactgtacct
    20041 aattgcttga tcactacttc ttctacttta tcgtttgtaa atacgatcat gatcgttgga
    20101 tctttttctt cgttttggat cacatcaaga tccatctcag ctaatttcgt tcctgttcct
    20161 acttcttggc cttctttgac agcaatctca aaagctggct gattcatttg gactgtatcg
    20221 attcccatat ggatcaaaac ttcgatacca tctttcgttt ccagcccaat tgcatgtttg
    20281 ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa caacttctct tgatgttgga
    20341 ataacagcaa agccgtcacc catcattttt tgtgagaata ccggatcttt tacttcagat
    20401 aaaggaatca cttctccaga agctgcggaa actagttctt gttctatacc taagtattgc
    20461 ttactacctg cttgtccatt tcctgtagaa accatttctg gctctggaat ctctgctcct
    20521 gattgcagca gatcttcgat atctgatttc aagacatctg cttttggtcc ataaactgct
    20581 tggactccat tgtctttgac gatcaatccc atcgcccctg ctcgtttcca ggcttcttca
    20641 gtccctactt tttctcgatc atttacactt acgcgtaacc gagtcataca agcatcgaca
    20701 tctttgatat tttcttttcc tcccagcaaa tgaatgatct ggaccacttg ctgatctgct
    20761 gttcctgttc cgctgttaga tacaacggtt tcttcactgt cattgtcgta atttccgtta
    20821 cgtcccggtg tagcaaagtt gaattttttg atcaagaaat ttgctaagaa atacgtaaca
    20881 acaccaaaag cgatcacaca caacacgaag ttgataagat ctccacctag acccgctttg
    20941 atagctaacg gtgtccgagt cagcaattcg atattcccaa atgaatggac ccgcaatggc
    21001 aagatatctg ccatcgcaaa tgcagcccct tggatcactg aataaatgac atataatggc
    21061 acagcagcaa acataaacat gaactccaat ggttctgtta cacctgttag aaagacagcc
    21121 aacgctgctg aaaaatacat tgatttatat cttgctttct tatcaggatc gacattacga
    21181 tacatcgcaa aagccattcc catcaagata cctgaagaac caatcatttg tcctactttg
    21241 aaacgagccg gcgtccagtt atgcaacaca aactcatatt gcgacatatc ccctgcacct
    21301 ttaagattca ctaaatcagt ggcccaagcc aaccataacg gatcttgccc aaatacttgt
    21361 gtccccgcct gtgcgcctga taaaatctca tacgatccgc caagctgtgt atagttgata
    21421 ggaatcgtca gcatatgatg caatccaaat ggcaatagta aacgttctaa tgttccaaat
    21481 aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg caatccataa accaaaatta
    21541 ttgattccag cctgtacgtt tggccacacc acagcaagaa tcaatgccac gattgttgac
    21601 cataagataa caacaaacgg aacaaagcgc tttccattga aaaatgacaa agcatccggc
    21661 aattttctat aattatagta cttattataa gccattgccc cgacaaatcc agcgatgatc
    21721 cctacgaata ctcccatatt caaagctggt gcttccaata cacttgtaaa aaatccttta
    21781 accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat cttcattcgc aagcatgtca
    21841 cttgtgacac caaagatagc cccagtgatc cgattgatta aaataaatga gatcccagct
    21901 gcaaatgctc cgcctgctct ttcttttgcc caacttcctc ctattgctaa agcaaataaa
    21961 atatgaagat taccaataat tgcccaacca atattttcaa ttactccgcc cgttgtgaca
    22021 agcggcgtcc aatcaggatt gatcaaggga attgatttcc ctatactgat catcaaccca
    22081 gctgccggca taactgctac cacaaccatt aaagcctttc caaatttttg ccaaaactca
    22141 aaactaaata tctttttcat tccgtttagc tcctttacgc aatcggtttc gttaaacgta
    22201 ttatagcgtg cgttttcttt tttgtaaatg ctttattttt tatttttctg tctataccat
    22261 tctataaaac ctataatacg tgtgttccct tctataattt tctcctaaaa atttattttt
    22321 ttaagctttt ttctatgaaa tattttcttt tgtctattga cttgtgcaat cggttgcgtt
    22381 tattatgaac gtataaaaca gctcattagg aggagaaaaa tgaaacagat caaacgacta
    22441 tttgctattg atccatggaa aatcagcacg acacaattag ataaaaaaaa tcttcgactg
    22501 caagaatctt taacaagtat tggaaacggt tatatgggga tgcgcgggaa ctttgaagaa
    22561 acttactcag gggaccacca tcaaggtact tatttggcgg gtgtctggta tcccgataag
    22621 acacgtgtcg gctggtggaa aaatggttat ccagaatact ttggaaaagt gatcaacgcc
    22681 ttgaacttta tcgcgataga tatttcgatc aacgatcagc tgatcgactt ggcttctttg
    22741 gaaccagaag acttttactg ggaattagat atgaaaaatg gtgtattatc tcgtacattc
    22801 acaatcacta cttctactaa taaagtacgc atttccttcg aacgttttct gagcattgtc
    22861 aaaaaagaat cagcttatat ctatttaact atagaaatgc ttgaaggtag cggaacagtc
    22921 aaactcgtat caaaattgga tggaaatgtt caaaacgaag acagcaacta tgatgaacat
    22981 ttttggaatg agattgatcg tggaactata gaagaaatcg gctatttgac gactaaaact
    23041 attccaaaca actttgggat tgaacaattt actgttactg cagcaatgcg ccatttcgtt
    23101 aatggaaaat cagtcatccc tgactatcac gaagatactt tagcattaca tggttgcttg
    23161 tctgcagaat tatctcaagg tgagacatgg tcagtggaaa aagaaattat cgttcttact
    23221 agtagagata ttccagaagc gcagcaagca gaaaaagctg tctcagaatt aacgaaccag
    23281 acaaacaatt atatccaagc aaaagaagag caaacagcgg cttgggcaga acgatggaag
    23341 cttgccgatg tggtcatcga aggagacgaa gaagcacaac aaggtattcg attcaatttg
    23401 ttccaactat tctctactta ctacggcgaa gatgaacgat tgaatatcgg ccccaaagga
    23461 ttcacaggag aaaaatacgg cggagccact tactgggata cagaagcgta tgctgtccct
    23521 ctttatcttg ctttagccaa acctgaagta actaagaatc tgctgaaata tcggcataat
    23581 caactgccgc aagcaatcca caacgctcag caacaaggat taaaaggcgc gctttatcct
    23641 atggtcacct tcactggtgt cgaatgccat aacgagtggg aaatcacatt cgaggagatt
    23701 caccgaaatg gcgcgattgc ctatgcaatc tacaattata cgaattacac cggcgatacg
    23761 gaatatttga aaaatgaagg actggaagtt ttagcagaaa tcgcacgctt ctgggcagac
    23821 cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc acggtgtgac cggcccaaac
    23881 gaatatgaga acaatatcaa taacaattgg tatacaaata cgatcgcagc ttgggtcttg
    23941 cgttacacgc gagaaaatta tttaacttat caaaaagaag ctgctatcac tatcagcgaa
    24001 gaagaactag ccaaatggaa agatatcgca gagaacatgt acttcccagt tgacgaagaa
    24061 ttaggaatct ttgtccagca tgacacattc ttagataaag atctcatgcc tgtatccgat
    24121 attcccacaa gcgatcttcc tctgaatcag cattggtctt gggacaaaat ccttagatct
    24181 tgcttcatca agcaggcaga tgtactacag gggatttatt tcttcaatga tacttttaca
    24241 aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa tgactgtcca tgagtcttcc
    24301 ctttcgccaa gtatccattc gattttagca gcagaattag gaatggaaag caaagctgta
    24361 gaaatgtacc agcgaacagc tcgcttggat ttggataatt acaataacga cacagaagac
    24421 ggcttgcaca tcacttccat gactggtagc tggttgtcga ttgtccaagg atttgctcaa
    24481 atgaaaactg cccatgatca gttgagattc gcaccatttc tgccaaaaac ttggaaagct
    24541 tattctttcc atatcaatta tcgcggacgt ttattgttca tctcagtcac agatgaacaa
    24601 gttactttcg agttattaga aggttctcct ctagaaattt acgtctatga acagtcttat
    24661 tctttagcgg atcgtctaga aataacgcta agaaaggagg aagcaaatgt ttaaaggggt
    24721 attgtttgat ttagacggtg tcattaccga tactgctgaa ttccattatc gtgcttggcg
    24781 aaagcttggt caggaaatcg gcattacgat cgaccacaca ttcaacgaac agctaaaagg
    24841 cgtcagccga gaagactcac tctctttgct tttagcttat ggcggaaaag aacactcttt
    24901 ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac gattattatt tagagatgat
    24961 ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg cctttactgg attctttaaa
    25021 agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa aacgggccat tcctacttga
    25081 aaaaatgggt cttacacctt actttgatgc tattgccgac ccggcagaag caacaaacgg
    25141 aaaacctgcg ccagatatct tcctccttgc agcaaaagct gtcggactaa aagcagaaga
    25201 atgtatcggg atcgaagacg cgcaagccgg tatccaagcc atcctctcaa gtggcgctca
    25261 accaattggc gtgggacgtg ctgaagacct tggagaggag atcccgcttg tccctgatac
    25321 gacttttctt acgatcgatt acttgaaaga aaagtggcat gaccatggat aatccagaga
    25381 tctccgtcgt accagttcca gagactgaaa taaagaaaat cacattgaag aacgactact
    25441 tagaagtcgt tcttctcaat tacggtgccc gcctgcacca aatcttcgca ccagacaaag
    25501 aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt cgaagatgtt ctaacagaca
    25561 aaagcttttt tggagcaact gtcggacctg tagccggaag gatccgggat gcttcttggg
    25621 gtgagcatag attggagaaa aactgcggat cacaccatat ccacggtggc acgaaaggct
    25681 ggtctttcca attttgggaa gtagaagttt tcaaaactcc atcatcaata ggtgtggtct
    25741 tttacttaaa agatgaattt tctacctatc caggaccaat cactgctacg atcacttacc
    25801 gtttaacaaa aaacgaatta gagatgacaa cctctgctag tagccttgtt gaaaccatct
    25861 gcaatccgac caaccatgcc tacttcaacc tttcaggaaa tggaacaaga gacatctatg
    25921 aacatcagtt aacggtcttt ttagatggtg tgcttgaatt agatcaagaa aaactgccta
    25981 ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt tagaaaatcc ccaacactgc
    26041 aagagatcct tgcatgttat ccagatggac tagatgatgt atttcttttg catcatccta
    26101 ggctttccaa aacttcctta caattattcg agaagcattc tggtcgccaa atgactatag
    26161 caacttctaa taaaagtatg gtattatttt ctacgactgg attcgaagct gatttctctg
    26221 tcaatggaaa acagatgcat tcaaactacg gattagcaat cgaaccgcaa gaaatccctg
    26281 atatcgttca tttccccaaa tttggatcga tcaacctcca tcctggtcaa gaacggatca
    26341 gtcagacgat ttatcggttc tctgcacaat aacaagagaa aggtgttttt atcatggcta
    26401 ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac ctcgaccgtc tctcgtgtca
    26461 tcaacgatca tcctagtatt tccgagacaa caaagaagaa agtccgaaaa attatggatg
    26521 aactaggtta tgttcctaac atgactgccc gcaatctagg aaaacgcatt tccagtgcca
    26581 tcggcgtaat cttaccccct cttgattcaa aagagcggat cggtaaccca ttttatcttg
    26641 agatcatgga agcaatcaac gaagaagcgc ggaattatga tatgactaca gcaatcgcta
    26701 ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg tatgcattta caaaaacaag
    26761 tagatggttt tattctagtg tattcagata gtcaagatac tgtcattgat tatttatacc
    26821 aaaaccacat ccctttcact ttgatcggac agccttatca tcatgagaac gaaatcgttt
    26881 atgtagataa tgataaccag cttctaggta aacaagcaac agaatttttg atccagaatg
    26941 gacataagaa tatcctgttt gtgacaaaca cgactcatga aagtttgtat ttcgaacgat
    27001 atttcggtta tcaaaaagca atgatgatgg ctggtcttcc tgcgttatcc agtgtagtac
    27061 tagagcatcc ggaggattat ctgtcctttg agaaaacgtt gaaagaaaac gaagccacag
    27121 cattggtggt cattgacgat atcttcgccc ttcgcacgat gcagttggcg caaatgtatg
    27181 gctatgtcat tccagagaca ctctccgtca ttagcttcaa caactcgatc ttctcaacat
    27241 taacacaccc ttaccttaca agtatcgata tcgatatctc cgaattagga cgtgtcgcta
    27301 tgcaaaagct gcgcgaattg atccatgaag aattgagcag tggcgtacgt ttggtcatcc
    27361 ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga aaacccatac taaatagctg
    27421 actcactaaa tacgctgacc atttatccta tggtcagctt tttatgtaaa agaagatact
    27481 acttctaaca cattgtttat ctgtaaaaaa gagcgtggga caaaagtaaa gaacactttt
    27541 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc
    27601 tgaaattcac aaaaatttaa aaagcaattt tcttgagttt cttcttattt cttggagtta
    27661 aacgcttctg tcccagcctc ttttttcctt ccttcaattt tttacacgtc gacgattcaa
    27721 taaaaaaccg cctaatattg taaagacagg tgcaagaatc gagaaaaagg cgaatggcaa
    27781 gtaatgcaac gcgctaactt tcatcgttcc cataatgaat gtgccgctga ctccccacgg
    27841 tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt cgtgtcaaat aggtttttga
    27901 taagccaacg tcatcaaaag aacgcttgaa tgtttctcct ggaagaatga tggacagata
    27961 ctgttctcca acgatcagat tgattccgat acagctcaag gcagtaaacc cgactaaacg
    28021 agaaggtcgg tccatctttt cttttaattc ttgaacgatg gtatcaacga tcagatattt
    28081 gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa ctagcagaac ctaacatact
    28141 catgatcccg cctcgagaaa gcagttcatc tagctttgga tcaggtgtat ttgcgacata
    28201 accattcatc aagatgtctg ctgcttttcc gaccgtcaaa cttttctcat ggataaaaga
    28261 aagaacgaga gcagctgtcg atcccgctag taatgtaggg atagccggaa tttttttgat
    28321 tgccccccca aataaaataa ctagtggaat caatgtccaa ggagagatcc aaaagctcgc
    28381 tttcatgttt tggatcattt cattgatact agctagatcg gctgaatgac tgttatgtcc
    28441 taatatcgta taaaaaatca acgaaaggac gaatgctggg aaatccgtat aacgcatacc
    28501 agcgatatgt tcgaatagat taactcctcc aatacccgct gccaagttgg tcgtatctga
    28561 taaaggcgag cagttattcc ctaaaaaggc accggaaacg actgcgcctg tagtcattgc
    28621 atcattgaat ccaagtaaat gaccgatccc caaaaaagca attccaatcg tcgaaatcgt
    28681 tgtaaatgaa ctgccgacag tcactccgac aattgcacaa acaagaaatg ctgtcggtaa
    28741 aaagaaacgc acagaaacaa gtttaaatcc ataaaccata atcgtaggga tggttccaga
    28801 taaaatccat gaagcaacta agacaccaat caaaagaaaa atcaggattg gaatgatccc
    28861 tggagtgatt cctttgatga tcccgtcatg gatctcatcc catgtgaact ttttaaatct
    28921 gccataaaac aacaaacaca tgaaagctgc aaggatcggg atatgtggcg agagctggaa
    28981 tccgatgatc agtactccta ggagtgccaa caacacgatc aatacagcga tactttcttt
    29041 taaagaaata tttttattca tcgtcgatct cctatttcaa atgaaaaggc cctgccatga
    29101 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
    29161 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
    29221 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
    29281 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
    29341 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
    29401 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
    29461 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
    29521 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
    29581 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
    29641 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
    29701 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
    29761 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
    29821 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
    29881 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
    29941 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
    30001 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
    30061 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
    30121 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
    30181 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
    30241 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
    30301 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
    30361 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
    30421 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
    30481 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
    30541 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
    30601 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
    30661 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
    30721 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
    30781 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
    30841 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
    30901 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
    30961 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
    31021 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
    31081 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
    31141 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
    31201 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
    31261 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
    31321 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
    31381 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
    31441 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
    31501 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
    31561 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
    31621 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
    31681 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
    31741 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
    31801 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
    31861 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
    31921 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
    31981 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
    32041 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
    32101 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
    32161 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
    32221 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
    32281 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
    32341 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
    32401 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
    32461 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
    32521 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
    32581 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
    32641 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
    32701 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
    32761 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
    32821 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
    32881 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
    32941 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
    33001 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
    33061 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
    33121 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
    33181 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
    33241 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
    33301 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
    33361 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
    33421 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
    33481 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
    33541 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
    33601 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
    33661 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
    33721 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
    33781 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
    33841 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
    33901 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
    33961 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
    34021 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
    34081 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
    34141 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
    34201 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
    34261 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatat gtaattaatt
    34321 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
    34381 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
    34441 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
    34501 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
    34561 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
    34621 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
    34681 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
    34741 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
    34801 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
    34861 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
    34921 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa
    34981 agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta
    35041 cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac
    35101 acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc
    35161 tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca
    35221 ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc
    35281 gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa
    35341 agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt
    35401 ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag
    35461 ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga
    35521 agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga
    35581 tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg
    35641 ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga
    35701 agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg
    35761 gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg
    35821 gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc
    35881 agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag
    35941 tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg
    36001 tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt
    36061 agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga
    36121 agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt
    36181 tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag
    36241 gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat
    36301 tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac
    36361 ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt
    36421 atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa
    36481 atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc
    36541 aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa
    36601 atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt
    36661 aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc
    36721 ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc
    36781 ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata
    36841 atat
//
LOCUS       DADVXO010000012        34703 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.012, whole genome shotgun sequence.
ACCESSION   DADVXO010000012 DADVXO010000000
VERSION     DADVXO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.012"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(451..1761)
                     /locus_tag="KB950_000537"
     CDS             complement(451..1761)
                     /locus_tag="KB950_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBB6856777.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="KB950_000538"
     CDS             complement(1773..3191)
                     /locus_tag="KB950_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6856778.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="KB950_000539"
     CDS             complement(3431..4999)
                     /locus_tag="KB950_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBB6856779.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="KB950_000540"
     CDS             complement(5003..5413)
                     /locus_tag="KB950_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBB6856780.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="KB950_000541"
     CDS             complement(5410..6333)
                     /locus_tag="KB950_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBB6856781.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="KB950_000542"
     CDS             complement(6330..7208)
                     /locus_tag="KB950_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBB6856782.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="KB950_000543"
     CDS             complement(7195..8532)
                     /locus_tag="KB950_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBB6856783.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="KB950_000544"
     CDS             complement(8535..9638)
                     /locus_tag="KB950_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6856784.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="KB950_000545"
     CDS             complement(9712..11220)
                     /locus_tag="KB950_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6856785.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="KB950_000546"
     CDS             complement(11186..11878)
                     /locus_tag="KB950_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6856786.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="KB950_000547"
     CDS             complement(11900..12874)
                     /locus_tag="KB950_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6856787.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="KB950_000548"
     CDS             13174..14046
                     /locus_tag="KB950_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6856788.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="KB950_000549"
     CDS             complement(14504..16702)
                     /locus_tag="KB950_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6856789.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="KB950_000550"
     CDS             16934..17926
                     /locus_tag="KB950_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6856790.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="KB950_000551"
     CDS             17986..19407
                     /locus_tag="KB950_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBB6856791.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="KB950_000552"
     CDS             19400..20899
                     /locus_tag="KB950_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBB6856792.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="KB950_000553"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="KB950_000553"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBB6856793.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="KB950_000554"
     CDS             22491..23174
                     /locus_tag="KB950_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBB6856794.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="KB950_000555"
     CDS             23176..24012
                     /locus_tag="KB950_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6856795.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="KB950_000556"
     CDS             24023..25777
                     /locus_tag="KB950_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6856796.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="KB950_000557"
     CDS             complement(26053..26394)
                     /locus_tag="KB950_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6856797.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="KB950_000558"
     CDS             complement(26462..28633)
                     /locus_tag="KB950_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBB6856798.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="KB950_000559"
     CDS             28802..29701
                     /locus_tag="KB950_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6856799.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="KB950_000560"
     CDS             complement(29772..30629)
                     /locus_tag="KB950_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6856800.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="KB950_000561"
     CDS             complement(30614..33316)
                     /locus_tag="KB950_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBB6856801.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="KB950_000562"
     CDS             complement(33329..34618)
                     /locus_tag="KB950_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBB6856802.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT        10188 a         7173 c         6107 g        11235 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcagggc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagcatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taaaccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctt
//
LOCUS       DADVXO010000013        34664 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.013, whole genome shotgun sequence.
ACCESSION   DADVXO010000013 DADVXO010000000
VERSION     DADVXO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.013"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="KB950_000563"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="KB950_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBB6856803.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="KB950_000564"
     CDS             complement(2020..2922)
                     /locus_tag="KB950_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6856804.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="KB950_000565"
     CDS             complement(2919..3611)
                     /locus_tag="KB950_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBB6856805.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="KB950_000566"
     CDS             complement(3654..4295)
                     /locus_tag="KB950_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6856806.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="KB950_000567"
     CDS             complement(4333..4647)
                     /locus_tag="KB950_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856807.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="KB950_000568"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="KB950_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="KB950_000569"
     CDS             complement(5984..6916)
                     /locus_tag="KB950_000569"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBB6856808.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="KB950_000570"
     CDS             complement(6913..7689)
                     /locus_tag="KB950_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBB6856809.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="KB950_000571"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="KB950_000571"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBB6856810.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="KB950_000572"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="KB950_000572"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBB6856811.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="KB950_000573"
     CDS             complement(12014..13294)
                     /locus_tag="KB950_000573"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBB6856812.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="KB950_000574"
     CDS             complement(13313..14239)
                     /locus_tag="KB950_000574"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBB6856813.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="KB950_000575"
     CDS             complement(14252..15535)
                     /locus_tag="KB950_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBB6856814.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="KB950_000576"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="KB950_000576"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBB6856815.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="KB950_000577"
     CDS             complement(16323..17234)
                     /locus_tag="KB950_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6856816.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="KB950_000578"
     CDS             complement(17231..17695)
                     /locus_tag="KB950_000578"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBB6856817.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="KB950_000579"
     CDS             complement(17700..18398)
                     /locus_tag="KB950_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6856818.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="KB950_000580"
     CDS             complement(18385..18771)
                     /locus_tag="KB950_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856819.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="KB950_000581"
     CDS             complement(18918..20588)
                     /locus_tag="KB950_000581"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBB6856820.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="KB950_000582"
     CDS             20771..21004
                     /locus_tag="KB950_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBB6856821.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="KB950_000583"
     CDS             complement(21103..21534)
                     /locus_tag="KB950_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBB6856822.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="KB950_000584"
     CDS             21613..22026
                     /locus_tag="KB950_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBB6856823.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="KB950_000585"
     CDS             complement(22064..23212)
                     /locus_tag="KB950_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6856824.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="KB950_000586"
     CDS             complement(23334..23930)
                     /locus_tag="KB950_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6856825.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="KB950_000587"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="KB950_000587"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBB6856826.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="KB950_000588"
     CDS             27160..27591
                     /locus_tag="KB950_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6856827.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="KB950_000589"
     CDS             complement(27636..28985)
                     /locus_tag="KB950_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6856828.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="KB950_000590"
     CDS             complement(29055..30218)
                     /locus_tag="KB950_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBB6856829.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="KB950_000591"
     CDS             30347..30670
                     /locus_tag="KB950_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856830.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="KB950_000592"
     CDS             complement(30853..32202)
                     /locus_tag="KB950_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6856831.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="KB950_000593"
     CDS             complement(32271..33224)
                     /locus_tag="KB950_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBB6856832.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="KB950_000594"
     CDS             complement(33326..34516)
                     /locus_tag="KB950_000594"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBB6856833.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9848 a         7593 c         6088 g        11135 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat cttt
//
LOCUS       DADVXO010000014        33344 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.014, whole genome shotgun sequence.
ACCESSION   DADVXO010000014 DADVXO010000000
VERSION     DADVXO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.014"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            201..1757
                     /gene="rny"
                     /locus_tag="KB950_000595"
     CDS             201..1757
                     /gene="rny"
                     /locus_tag="KB950_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBB6856834.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1836..2642)
                     /gene="proC"
                     /locus_tag="KB950_000596"
     CDS             complement(1836..2642)
                     /gene="proC"
                     /locus_tag="KB950_000596"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBB6856835.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2783..5356
                     /gene="mprF"
                     /locus_tag="KB950_000597"
     CDS             2783..5356
                     /gene="mprF"
                     /locus_tag="KB950_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBB6856836.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5498..6361
                     /locus_tag="KB950_000598"
     CDS             5498..6361
                     /locus_tag="KB950_000598"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBB6856837.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            6606..7544
                     /locus_tag="KB950_000599"
     CDS             6606..7544
                     /locus_tag="KB950_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBB6856838.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7567..8247
                     /locus_tag="KB950_000600"
     CDS             7567..8247
                     /locus_tag="KB950_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6856839.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8220..9044
                     /locus_tag="KB950_000601"
     CDS             8220..9044
                     /locus_tag="KB950_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6856840.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9211..10029
                     /gene="purR"
                     /locus_tag="KB950_000602"
     CDS             9211..10029
                     /gene="purR"
                     /locus_tag="KB950_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBB6856841.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10532..11905
                     /gene="glmU"
                     /locus_tag="KB950_000603"
     CDS             10532..11905
                     /gene="glmU"
                     /locus_tag="KB950_000603"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBB6856842.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            11999..12970
                     /locus_tag="KB950_000604"
     CDS             11999..12970
                     /locus_tag="KB950_000604"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBB6856843.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      12976..13078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13144..13713
                     /gene="thiT"
                     /locus_tag="KB950_000605"
     CDS             13144..13713
                     /gene="thiT"
                     /locus_tag="KB950_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBB6856844.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13794..14567)
                     /locus_tag="KB950_000606"
     CDS             complement(13794..14567)
                     /locus_tag="KB950_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856845.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14834..16534
                     /locus_tag="KB950_000607"
     CDS             14834..16534
                     /locus_tag="KB950_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBB6856846.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16504..16722
                     /locus_tag="KB950_000608"
     CDS             16504..16722
                     /locus_tag="KB950_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBB6856847.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16742..17632
                     /locus_tag="KB950_000609"
     CDS             16742..17632
                     /locus_tag="KB950_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBB6856848.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17650..17934
                     /locus_tag="KB950_000610"
     CDS             17650..17934
                     /locus_tag="KB950_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856849.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18421..19035
                     /gene="gmk"
                     /locus_tag="KB950_000611"
     CDS             18421..19035
                     /gene="gmk"
                     /locus_tag="KB950_000611"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBB6856850.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            19040..19345
                     /locus_tag="KB950_000612"
     CDS             19040..19345
                     /locus_tag="KB950_000612"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6856851.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19598..22006
                     /gene="priA"
                     /locus_tag="KB950_000613"
     CDS             19598..22006
                     /gene="priA"
                     /locus_tag="KB950_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBB6856852.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            22021..22512
                     /gene="def"
                     /locus_tag="KB950_000614"
     CDS             22021..22512
                     /gene="def"
                     /locus_tag="KB950_000614"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBB6856853.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22505..23443
                     /locus_tag="KB950_000615"
     CDS             22505..23443
                     /locus_tag="KB950_000615"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBB6856854.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23443..24801
                     /gene="rsmB"
                     /locus_tag="KB950_000616"
     CDS             23443..24801
                     /gene="rsmB"
                     /locus_tag="KB950_000616"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBB6856855.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24814..25554
                     /locus_tag="KB950_000617"
     CDS             24814..25554
                     /locus_tag="KB950_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBB6856856.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25551..27620
                     /gene="pknB"
                     /locus_tag="KB950_000618"
     CDS             25551..27620
                     /gene="pknB"
                     /locus_tag="KB950_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBB6856857.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27785..28684
                     /gene="rsgA"
                     /locus_tag="KB950_000619"
     CDS             27785..28684
                     /gene="rsgA"
                     /locus_tag="KB950_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBB6856858.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28697..29347
                     /locus_tag="KB950_000620"
     CDS             28697..29347
                     /locus_tag="KB950_000620"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBB6856859.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29348..29986
                     /locus_tag="KB950_000621"
     CDS             29348..29986
                     /locus_tag="KB950_000621"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBB6856860.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30141..30995
                     /gene="mreC"
                     /locus_tag="KB950_000622"
     CDS             30141..30995
                     /gene="mreC"
                     /locus_tag="KB950_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBB6856861.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            30999..31508
                     /gene="mreD"
                     /locus_tag="KB950_000623"
     CDS             30999..31508
                     /gene="mreD"
                     /locus_tag="KB950_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBB6856862.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            31763..33337
                     /locus_tag="KB950_000624"
     CDS             31763..33337
                     /locus_tag="KB950_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6856863.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        10987 a         5967 c         7219 g         9171 t
ORIGIN      
        1 aaccatcgtt taatgaataa gtagaaagcc gtttctttgt ttatttaaag ttgacactac
       61 ggggtacaaa cattagaata aagttgtgca tgttttttgt acacatatat gcgaagatgc
      121 atattgacga gtttagaata atttatcata gaagctgtat agatgataaa aatgaaaaag
      181 atagtatgga ggtggatttt atggtattca acattctcct cgctatcatc ggtttaattg
      241 tcggtcttgg tttagggttt atggtcgcaa agtcacgtca cgagaaagaa atagctggtg
      301 cacaatcatc agctgctgga atcatcgata gtgcaaaaaa agaagcagaa actctaaaaa
      361 aagaagcatt gttagaagct aaggaagaaa atcagaaata ccgttctgaa gttgaaagtg
      421 agctgagaga aagtaagtta gaattaaaat ctcaagagaa tcgtttgatt caacgcgaac
      481 aaacgctgaa tcgtaaagat gactcgttag aaaaacgcga gaactcactt gaaagtaaag
      541 aagaagcact tagttcaaaa cagcaattaa ttgaagagcg agaaaaagac gttgaaaaaa
      601 tgattgaaga tcaacaaaaa gaactagaaa aaattgctgg tttatcaaaa gaggacgcac
      661 gagatgtcat catgaaatcg actgaagaag agctgaatca tgagttaact ctaatggtaa
      721 aagaatctga acagcgtgcc aaagaagaag cagatcgcaa agcgaaaaac ttattatcat
      781 tagcgattca acgctgcgca gcagatcaag tttcagaaac gactgtttca gtcgttaccc
      841 ttccgaatga tgaaatgaaa ggtcgtatca ttggacgaga aggtcgtaat attcgaacgc
      901 ttgagacgct gactggtatc gatttaatca tcgatgatac accggaagca gttgttcttt
      961 ccggattcga tccaatccga agagaaatcg cacggatgac cttggaaaaa ctgatacaag
     1021 atggacgaat ccatccagcg cgtatcgaag aaatggtcga gaaatctcgt aaagaaatgg
     1081 atgaacgtat tcgcgaatac ggggaagagg cagcgtttga agtcggtgct catacacttc
     1141 atccagatct aatcaagatc atcggacgtc tgcgattcag aactagttat ggacaaaacg
     1201 tcttgaagca ttcggtcgag gttgcaaaat tagctggtat cttagcagca gagcttggcg
     1261 aagatatcca attagcaaaa cgtgccgggt tgctccatga tattgggaaa gcacttgacc
     1321 atgagattga aggatctcac gttgagatcg gtgcggaatt agctgccaag tacaaagaaa
     1381 acccagtagt aatcaacgcg atcgcttctc atcatggaga tgtggaagct acttctgtta
     1441 tctctgtcct tgtagctgca gcagatgcgc tctcagcagc aagacctggt gcgcgaagtg
     1501 aatcattaga aaactacatc cgtcgtttaa gaagtctgga aaatatttcg aacagcttcg
     1561 ccggtgtaga atcaagttac gcagttcaag ctggacgcga agttcgggta atggtcaagc
     1621 cagaagaaat ctcggattta gacgctgttc gtttagtacg tgatattcgt aaacgaatcg
     1681 aagaagaact ggattatcct ggacacatca aagtaatcgt tattcgggaa actcgtgcaa
     1741 ctgattatgc taaataataa aaaatacaaa agaagagccg cggcaaaagc cacagctctt
     1801 ctttttttgt aaggcacgaa aaaacattgc tatcattatt ttttattcaa catctcaaga
     1861 tctttctcga tcgtcgtatt gattcctttg atgattgctg ggatgaatcc tgcttcttct
     1921 aaggcaacta cacctgcaac agttgtaccg ccaggagaag aaacttggtc tacaagttcc
     1981 catggatttt tatcacttga aaggaatttt tgacaagaac ccataattgc ttttgcagca
     2041 atctcagtgg ctgtctgttt ggacaaacca ttcagtacac cagtccgtcc catcgcatcg
     2101 atcagcatgt aaacgaatgc gggtgagctg ccagcaaggc caatgaatgt actgaaatct
     2161 gattctgcta aaggatagac gctgccgatt gcttcaaaaa tcatcgtagc ttgctgaagc
     2221 tgttcctccg tggcagcttc atttttgcaa atcgcagaaa cgcctgctcc tacgctgaca
     2281 ttgacattcg gcatcacacg tacgatggag gcctgaggat cttcaagcgc tgtttctaaa
     2341 tctattaatg attttcctga agcaatagaa atgaataaga ctttctggct atattctctt
     2401 agttcttcca atactttttc aatgattttt ggtttcacgg ctaaaagaac gatatctgat
     2461 tgtgatgcaa cttcctgatt acttttgcat gcatggacgc cagtttcagc agcaaatttt
     2521 tgtgcatttt caaaatgtcc actggaaacg aacacctctt ctttcgagac tgttcctttt
     2581 tttaataaac catcaataat agcttgtgcc atattccctg tgccgataaa tcctactttc
     2641 atgttatcct ctcctttagc attaggatat ctttcttttt ctatcatcgc aagcaaagaa
     2701 caggtctaaa gtggaaaaat taaaataaat aagataacat tggggtatga cacaaaagta
     2761 tacaagttag gaatgggaaa atgtgaaaaa taaacgatac ttgatgctgc aatggataaa
     2821 ggaacatagt ctgattttaa aactgatttt tttcggctcc gtgttagtct ttgtggcaaa
     2881 tcaagtcgca aatattgcta atggcatgag ttggcaagat atttggcaaa ggatgggaca
     2941 acaaagccgc atgacgatcc tgctcatgat tctggctgga ctgctgggtg tcactccgat
     3001 gttgttgtat gattgggtga cgatcagagt cttagaaaaa caaggaaaac caaaaatgga
     3061 aagaaaagag tttcttcttg ctgcgtgggt aacaaacacg atcaacaatc tcgccggttt
     3121 tggtggtgtg gtaggagcaa gtctgcgggc tagtttttat ggaaagcaga caaatagaaa
     3181 aatggtttta gccactgtat caaaagtcgc actatttatg ctgactggtt tgtctgtatg
     3241 gtcgtttctt acatttattg atgtcttttt tattcagtca gaaagtattt ttcgaagcta
     3301 ttggatctgg cttttcggcg gtagtttttt agcaccagtc attttctttt ttgcttatct
     3361 aaagagaaag acattgttct tcgagtttta tccaaaaggc ttgcttggtc tgctactggc
     3421 atcctttgga caatggacgg gagcccttgc ggtatttttg atcatcggca agttgatgag
     3481 aatagatatt tcgatgcttt cgatctatcc tatgttcatg attgctacat tgataggaat
     3541 gctgacgatg gtcccaggtg gaatggggac ttttgacgtt ttgatgatct taggaatgag
     3601 tcaattgtct gttcggcaag atgtagcagt agtttggcta ttatattatc ggttgtttta
     3661 ttatgttcta ccttttatga cgggacttct attgttcatc acacatacag gagtcaaaat
     3721 caatcgattt ttagataaca ttccgcgact gtttttccaa aaggttgcgc atatcgtgct
     3781 cgtaagtgct gtctattttg cggggatcat gatggtactt ctatcaacaa tcactaatct
     3841 gtctgcaatc agtcggctat ttcaagtatt attgccgttc tcattcaatt ttttagatca
     3901 gacagtgaat ctattggttg gctttttatt actcggcctt gctagagcac tttcagtaaa
     3961 agtcaaaaag gcatttctgc ctacgattgc tttactatct tttggtattt taaatacaat
     4021 cacacgtacg ttatcttggc agttgatatt ggtatatgtc ttgattctgt tagctgtgtg
     4081 gttatccaga aaagaatttt atcgaacaaa attcgtttat tcttggggag ctataatctt
     4141 tgatgctagc ttgttcggct ttttgtttat cgtttacgca attgcaggct atcacagcgg
     4201 acgcttttta gtcggtcgaa taacaggaaa tcattttatt ttgtttcctt cagatgatgt
     4261 gtggttctca gggcttattg gtttgggcat ctcattgcta gctttagtgg cgctttatca
     4321 atatttagct gaagatacta cgaagcttgg cagtgagtac gaaaaagacc gttttgaccg
     4381 tttattggaa acatacggag gcacagctac aagccatcag cttcgcttgt caggttacag
     4441 ttatttttat taccaagaag acgaccaaga tcaagtcgtt tttggttatc agatcaaagg
     4501 aaacaaatgc tttgttttag gagatccagt tggcaatcct aaaatgatgc gggcagcaac
     4561 gaatcagttt atgaaacaag cagattgttt aggctatcag cttgcgtttt ataagatatc
     4621 agaaagctac gtagtgatgc ttcatgaatt gggttttcac ttcacgaaag taggtgaagc
     4681 agggattgtt gatctagcaa cagaagactt gtctcggctg agtcagcaac ttgaatatca
     4741 acgtttgatg aaagaaggat accattttac ttggtacgat caactgccgg aagaattaca
     4801 tcaagctgta aatgaagttt cacaaaaatg gcttcaaggt gcacgagaaa aatacttctc
     4861 tgtgggacga tttgataaat catatgtatt atctagcggt gtcggtattg tccgtaaaga
     4921 tcaaaaagtc gttggcttca tcaccgctca gccgatgacg aagaagcagg cgggatatga
     4981 tttgttgcgg gtcttaccta aagaatctga ggaattagct gactatctgt tagccaatct
     5041 gtttattgta tacgagaaaa tgggctactc ggaagccaat ctaggcttag ctccattagc
     5101 taatgttgga gaaacagatt tttctttctt tcaagaaaaa gtcatgcata tcgtatataa
     5161 ttatggtaat tttttctatt ccttccagtc tgcttatgaa aagaaattaa aatatgtcga
     5221 tcattgggaa ggacgttatt ttgcttatag gaaaggaagt aatttcggat ttgctaccat
     5281 gcagctactc agtctgatag gaaaagaaaa aggaaatgtt ccgagtttgg cagaagaagt
     5341 gctcacagaa ttataagaat cactagaact tgtttggatt atgaagagaa aattctgtga
     5401 aagctgttga aacgatgaca aaatcaacca ctaggcagac ataagctata cttttatttt
     5461 tccctatgat agaatacttg cgacttagac ttttcgaatg ggtgagagga tggaattgat
     5521 tgaaaaagcg ccagccaaga taaatttagg actggatgta ttatatagac ggcaagatgg
     5581 gtatcatgag ttggaaatgg tgatgtcgag cgtagacttg gcagatcatc tattttttga
     5641 agaaataaca gaagacaaaa tcattgtaga aacaaataaa gcatttttac cggtagatga
     5701 acggaacaat gtttatcaag ctgctttatt gctaaaaaat aaatacaata tcaaaaccgg
     5761 tgtcaagatc tttgttaaga agaatattcc agtagcagct ggattaggcg gaggaagtac
     5821 agactgtgca gctgctctaa gagggatcaa ccaattatgg aatctgggct gttcgttaga
     5881 agaactggca gaaatcggtt tggaagtcgg tacagatgtt ccttactgtg tctacggaag
     5941 tacggcattt gtcggtggca aaggggaaga aatccaagta ttaccttcta tgccacagtg
     6001 ctgggttgtg ttggtcaaac caagaatgag tgtctctaca cgtacagtgt ttagagaagt
     6061 agatacgcaa gccatccaac atcaagacat ccacggactt gctgaagcca tccgagagca
     6121 agattatcag cagatgatca aaaaaatggg aaacagttta gaagatataa cgggaaagcg
     6181 gcatccagtc atccatcaaa tcaaagagcg tatgatcaag tacggagcag atgctgcatt
     6241 gatgagtgga agcggaccaa ccgtttttgc cttgtgtcaa cattattcaa gagcgcagag
     6301 agtatataat gggttaaaag gtttttgtga ggaagtttat ttagtacgaa ccctgcaata
     6361 aaatataaga agtaaaatag gtgatgaaaa actgaggaat cctaaaatgg tattcttcag
     6421 ttttttattg tagtgaaatg gctagatatg agcagcaact catccatttt acgatttaat
     6481 caagaatata catgcttaaa tcagaaattg atggatttct attgactgtg tttttttatt
     6541 ttgatacatt agttcacgtt aataaatcgt aaaattccga tttgttcatt gaaaggagca
     6601 tataaatgaa aaaatatctt ttgacagcta cggtaatgat aggtgcttta ctgtttgcag
     6661 catgtggaaa tacgaacaag gaagcagaca aaaaagagga tttgacgatc gttacgactt
     6721 tttatccgat gtatgatttt acaaaagaga tcgtagggga tgaaggaaac gtgaaacttt
     6781 tgatccctgc tggaacggag ccgcatgatt tcgaaccaag tgcaaaagaa agagcagaaa
     6841 tttccgatgc agatgttttc gtgtataata gttcagacat ggaatttttt gtggattcgt
     6901 taaaggattc agtggatagc aaacagacct tgatgatcga agcagctaaa ggaatcgacc
     6961 gtttagaatc gcaagaagca gacgaacatg aagaatcaga agaagggcat ggtcattcac
     7021 atgaatacga tccacatgtt tggctcgatc cggtattagc tataaaagaa gtcagaacga
     7081 ttgctgaaga attaggcgaa aaatatccag ataaaaagga aacttttacg aagaatgccg
     7141 atgcttatat caaaaagctt gaagcactgg atcaaaaata ctccgatgag ctaaaagatg
     7201 caacaaaccg cacatttgtc actcagcatg ctgcttttgc ttatttagcg aatcaatacg
     7261 acttaaaaca agtagcgatt tcgggcgttt caccagatca ggaacccaca ccaagtcgct
     7321 tagctgagct aaaagaattt gtgaagaaaa acaacatcaa agtgatttat tttgaagaaa
     7381 atgcctcttc gaaggtagct gagacattgt cgaatgaaac aggagtgaag ctagaagtgt
     7441 tgaatccttt agaaagcttg actaatgaac aaatcaaagc aggcgaaaat tatatttctg
     7501 tcatggaaaa aaatctgaaa gctttaaaag aaagtatcaa ttaagtttta gaatggaggt
     7561 tgtaaggtgc gttatatcga aatatctgat ttgacctttt actatgatga tgaacctgta
     7621 ctagaagatg tctcttatca tgtagatgcg ggtgaatttg tcattttaac tggagaaaat
     7681 ggagcagcca agtcaacctt ggtgaaagca tcgttaggat tactgaaacc ttcaaaagga
     7741 atgataaaga ttgctgagaa aaatcaagaa ggaaaaaaat taagtatcgg ttatattcct
     7801 caacaagttg catctttcaa tgcaggtttt cctagtacgg tgatcgaatt agtccgttca
     7861 ggaagatatc caagaggccg ctggttcaag cgtctctctg aaaaagatca tcaacatgta
     7921 cagcgtgctt tagaagctgt agggatgtgg gatatgcgaa atcggcgaat aggtgaattg
     7981 tctggcggac aaaagcagcg aatcagttta gcgcgtattt ttgctactga ccctgactta
     8041 tttgttcttg atgaaccaac aacaggtatg gatgaagaat ccagaaatga attttatcgt
     8101 cttctccgac atagtgcgca tgtccatggg aaatcagttc taatgatcac ccatgatcat
     8161 gaagatatca aacagtatgc ggacagacag atccgactgg tgagaaagga ggattctcaa
     8221 tggcgttgct ttcatatgaa ttcatgagaa gagcgtttct tgccgctatc ttcattgccg
     8281 gaatcgcccc gatgctaggt gtgtttttag tgatcagaag acaatcgctg atggctgata
     8341 cgctttctca tgtctcttta gcaggggtag cgttaggatt ctttttgaac cttaatccta
     8401 cacttacgac catgctagtc gttgtattag cagctgtgat attggagtat ctgcgaacga
     8461 tctaccgaac ctattcagaa atctccattg ccattttaat ggctggtggt ttggcgctag
     8521 cgttagtatt gatgaatttg agcggaggga attcagcaac aagtatccaa tcctatctgt
     8581 ttggttcgat cgtgaccatt acatgggagc aagtcatctt tttaggaatc ttattcgttg
     8641 ccatcggtat attgttcttt ttattcaaac ggccgatgta tgttctgact tttgatgaag
     8701 ataccgccca cgtggatggg ctacccgttc atttgatgtc gatgatgttc aatgtgatta
     8761 ctggtgtggc tattgctgtg atgatcccga ttgccggtgc acttttgata tccgcaatca
     8821 tggttcttcc cgcagcaatc ggtatgcgat taggcaaaag cttcaatgtc gtgattgcta
     8881 tcagtgtttg tgtaggattt ctagggatgc tgtcaggact aactagctcg ttttatctag
     8941 atacacctcc aggagcaaca atcacattag tctttattgc tttgttcctg attgtaaata
     9001 tcatcaaacg aatattggtt gccttcagaa gaaaaaataa ctaaattata aagaaaagaa
     9061 gccttataaa aacgaaaaat tttttatagg cttcttttta ttttaataaa taccgaacta
     9121 ttttcttatt ttttcataaa atgctcgaat ttttgaagaa aaacttatat aatgtaaaac
     9181 gataaaattt cttaaaagga gttggaatag gtgaaagtac gtcgcagtga acgtctagtt
     9241 gacatgacac agtatttatt gaatcacccg catgaattga tttccttaac aagctttgcc
     9301 gaacgttatg aatctgctaa atcttctatc agtgaagatt tgggaatcat caagaaaaca
     9361 tttaaagaac gagggtatgg aaccttggaa acaatcccgg gtgctgcagg tggtgtattg
     9421 ttcatccctg aaatttcata tgaagaggcg aaaaaatata ttgaatctct agcagaacgt
     9481 ctatcagaac aagaccgtct gcttccagga ggatatgttt atctctctga tttgttagga
     9541 gaacctgaac tgctgcgtca agttggaaga atcattgctt ctaaatacct tggtgaaaaa
     9601 atcgatgctg taatgactgt agccacaaaa ggcgtaccta ttgcccaagc tgtgtcctat
     9661 tatctgaatg cgccattcgt tattgtacgc cgggactcaa aaataacaga aggatcaaca
     9721 gtaagtgtca actatgtgtc tggatcttct gaaagaattg aaaagatgga actgtctaag
     9781 agaagtttga aacgtggttc acgtgtcctt gtagtggatg atttcatgaa aggcggcgga
     9841 acagtcaacg gaatgaagag cttgattgaa gaattcgaat cagaacttgt tggcatcact
     9901 gtatttgccg aatcaaaatt caacggtcat cgcgccatcg atgattatac gtcactgctt
     9961 tatgtcaaag atgtagatac tcaaacgaaa aatattacgg ttgtgccagg aaattatttt
    10021 tcagaataag aggttgtgaa aaagccgctt agtttcgagt agtaagaaaa attttgtgaa
    10081 aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca
    10141 ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga
    10201 aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa
    10261 aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc
    10321 aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag
    10381 agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac
    10441 gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
    10501 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
    10561 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg
    10621 taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat
    10681 tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata
    10741 tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt
    10801 ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga
    10861 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac
    10921 ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt
    10981 agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa
    11041 tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac
    11101 aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg
    11161 ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg
    11221 gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt
    11281 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg
    11341 tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga
    11401 ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
    11461 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
    11521 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
    11581 gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc
    11641 tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
    11701 gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat
    11761 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
    11821 tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
    11881 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
    11941 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
    12001 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
    12061 agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt
    12121 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
    12181 tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga
    12241 cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc
    12301 gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
    12361 gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca
    12421 aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt
    12481 cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt
    12541 cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg
    12601 ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac
    12661 ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
    12721 tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg
    12781 ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat
    12841 ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat
    12901 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa
    12961 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
    13021 ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt
    13081 tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
    13141 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
    13201 ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca
    13261 ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg
    13321 ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
    13381 gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa
    13441 aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg
    13501 attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg
    13561 tatgcactat gggggatgaa tccttggctc ttttctttcg taatgaatgg tgccagcggg
    13621 ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg
    13681 atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac
    13741 agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc
    13801 aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag
    13861 tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata
    13921 taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca
    13981 aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg
    14041 attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac
    14101 aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa
    14161 atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa
    14221 aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa
    14281 ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat
    14341 ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact
    14401 ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag
    14461 ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa
    14521 acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc
    14581 ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt
    14641 attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat
    14701 ttttgtgatt tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa
    14761 tctacaaagt cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa
    14821 aaaggagagc gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc
    14881 gcctatccag caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga
    14941 cgctttttgg cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac
    15001 cgtctatttt actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga
    15061 aggtgaaact tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt
    15121 tattaaagaa aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat
    15181 ttttcataaa aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac
    15241 agcacttgtt ttaggtggag gaggagcgca cggtgcttat cagattggtg tgtggcaggc
    15301 gctgaaggaa catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa
    15361 cggcgcattg attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc
    15421 gacaagacaa gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt
    15481 ctatcgagaa agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc
    15541 tccattggaa aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa
    15601 aatacgttta tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat
    15661 ccaacagaca aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc
    15721 cgctatggct tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa
    15781 aatacctatt gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca
    15841 agggccaggc ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca
    15901 aacattgtgg acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt
    15961 acaattaggc tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac
    16021 atttcattct gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa
    16081 agaaattcag ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa
    16141 tttatataag aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc
    16201 aaaaaaacat tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat
    16261 ctgccaacaa gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag
    16321 tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa
    16381 aactgtttat tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt
    16441 gcttaagcaa ccagttgaca cattactgat cctatattta tattatctca aggaggaaca
    16501 accatggcac aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac
    16561 aacaaggggt ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc
    16621 gatcttcgag attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac
    16681 gaagagtttg aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg
    16741 aatgaaaagt atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga
    16801 tattgaaata aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat
    16861 cctgaacttt gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg
    16921 aatcgaagta tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa
    16981 ctgggaattg attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg
    17041 tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc ttcgtgctgg acgaaaactt
    17101 cgtaacggta gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga
    17161 tacattgaaa agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt
    17221 agctttagtg ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag
    17281 ttttgtagaa atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt
    17341 tcttggagcg aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg
    17401 cggagatatt cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact
    17461 tatcgaagca caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg
    17521 ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca
    17581 gtctaagacg atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag
    17641 gtagagataa tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt
    17701 aaacgggata tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac
    17761 tataatgaga acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg
    17821 aaatttattg ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct
    17881 cgaaaagatg agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga
    17941 tgctgaacaa agaaatacaa taaataaaca gaattggatt tcttcgctta gcttctttgc
    18001 tctaggaata catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa
    18061 taagtgatgt tcaaaaacca ttttctcggt attaagccaa atttatgcaa aaatgtgaga
    18121 aactattttc acaaaatttt tcttcatact cgaaactaat cggcttcttc acgacctctt
    18181 atacacggcg ttcaaaagct tgcatctgcg ataataaccc caaccaaacg aaaaacatga
    18241 gaagctgttt atgtttggct gttcttatta cttgatgcag gacagctttt ttacgacctc
    18301 ttggtacagg gtagcttttt taccaatttc tagctataat taaagataac cttgaaagtt
    18361 gcagaaaaaa agtgcaaaat aaacgataag tacaaaaaga ctagttgaaa ggacgtcgtc
    18421 atgtcagagc gtggattatt aatcgtgttg tctggtccat ccggtgtagg aaaaggcaca
    18481 gtacgaaaag caatttttga gagtgaggat aatgattttc agtattcgat ctcaatgact
    18541 acccgtaaaa tgcgtgaagg ggaggtcgaa ggcgttgatt atttcttccg aagcaaagaa
    18601 gagtttgaat caatgatcga agctggagag atgctggaat acgcagaata tgtcgggaat
    18661 tattatggaa ctcctttgtc atatgtcaac aagacattgg atgaaggaaa agatgttttc
    18721 ttggagattg aagtccaagg agcaaaacaa gtcaaagaaa aagtgcccga tggtgtattc
    18781 atctttttga ctccgccaga tttagcggaa ttaaaatcac ggatcgtagg ccgaggaaca
    18841 gatgcagatg aagtaatcga aaaacgaatg aaagttgcaa aagaagaaat cgaaatgatg
    18901 gctctttacg actatgctgt tgtaaatgat gaagtgccgc tagcagttga acgaatcaaa
    18961 aacattattg ccagtgaaca ttttcgagta gatcgcgtaa tcggtaaata tattaaaatg
    19021 ttaaaggaga tgtagtctta tgatgttaaa accttcaatt gactcactat tagatcgtgt
    19081 aaattcaaaa tattctctag tgattttagc aagtaaacgc gcgcatgaat tggatgcaag
    19141 tgcacagcct actctagatt cttttgattc agtaaaaagc gtcggtcaag ctttagaaga
    19201 aatcgatgca ggaaatgtag tcaatgatcc acatcctgaa ttaaaacgtg aacgtttgaa
    19261 gatggaagaa gaggaacgtc aagcacaaaa agagcgtgaa caacacgaat tggaatcacg
    19321 tatccgtgaa gagcaaaaaa tgtaataaag aaaaatagag cgaacatcta aaaagaaaag
    19381 caaacccagc tgtttccttt tcaaacattc gccacttatt gtctggtaga aaaggctgtg
    19441 tctgaacttt ttgacacagt tctttttttt cttatctgct gttaattttt tgttaggatt
    19501 tggcagaaga taagacaaat ccatcgatct ctagtaaaat aggcaaggaa ctttttgtcg
    19561 cttgataaga aaagacaaaa aaaggaggaa acgaaccatg acattaacag ctgaagtcat
    19621 tgtggacgta cctactatgc agaccgatca gccattcact tatttggttc cctctgaaat
    19681 ggaaacagct ttgcaagtcg ggatgcgagt agaagtccct tttggtaatg gcaatcgtca
    19741 tgtccaagga tttgttatgg ctatccaaaa atcagaagaa tcagctaatc catctttgaa
    19801 ggcagttatt cgtttgctag accttgctcc ggtggtcaat gaagaattac tttcattagc
    19861 tgattatatg aaaaaaatta cttacgcatt caaaatcact tgtctacaaa cgatgcttcc
    19921 gagtgtgatg aaagcagaat atgataaatt gatctatcct ttggcagaca caccagaagt
    19981 tcaggtattg tttgatcagc gagaagtgat ttcctggaag gaagcagaag aagaaggcag
    20041 cctttctcaa ttgattcgct ggcgccaaga gcaattagta gatatcaaat atgaagtcca
    20101 tacacgaaat aaagtaaaaa cgattcgttt ggtccgttct ttgttaactg aaaagcaaat
    20161 cgaagaagaa tgggcaaaat tacggcaaaa tgccaagaaa caaaaagaac ttcttttgtg
    20221 tttatctgaa ctgagccaag aagaaccaat tgcttatttc aaaaataaag gaatcagtac
    20281 tgctgtcttg aatcaaggaa aagaaaaggg ctggctggag tttatagaat cagaacgtta
    20341 tcgcgaccct tataaagacc gagtatttga ccaaacgacg gcattggaac tgaatgcaga
    20401 acaaaaaaat gctgtcgaac aaatcatcac agcaggtcag cagcaaaaag atcaggtctt
    20461 tttgctggag ggaatcactg gcagcgggaa aacagaagta tatcttcagg caatcgctga
    20521 tgttttgtca gaaaataaaa cggctattat gttagtgcct gaaatcgcat tgactccgca
    20581 aatggtcgag cgattcaaag gacgcttcgg agaatcagtt gcggtcttac atagcggact
    20641 ttcccaaggt gaaaaatatg atgaatggcg caagattgag cgggaagaag cgcaagtagt
    20701 agtaggcgca cgatctgcta tttttgcccc gctgaaaaat atcgggctga tcattatcga
    20761 tgaagaacat gaatcaagtt acaaacaaga tgagacaccg cgttatcatg cgagagatct
    20821 agcaatttgg cgcagtaaat atcatcattg cccaatagtt ctaggaagcg cgacgccctc
    20881 tttagaatca agagcacgag ctcaaaaagg cgtttatcag ttattgcagt taaaccaccg
    20941 agccaaagca gctgcacagc ttccagctat tgagatcgta gatatgcgtg aagagtttca
    21001 gaatcatcgg acctctactt tttctgctaa tttgcaggaa aaaatacaaa atcgattaga
    21061 caaaaaagaa caaactgtcc tactgctcaa tcgtcgagga tattcgtctt ttgtgatgtg
    21121 tagggactgt ggatttgttc ttccatgtcc taattgcgat atctctctta cactgcatat
    21181 ggatacacgt tcaatgcgct gccattattg cggacatgaa gaacctattc ccaatcgctg
    21241 tcctaattgt ggcggcaata aaatccgcta ctatggaaca ggtactcaaa aagtagaaga
    21301 ggaactccga gaactttatc cgcaagctag gattttgcgt atggatgtag ataccactcg
    21361 aagaaaaggg gcacacgaac agattttaca aaaattcggt gcaaaagaag cagatatatt
    21421 attagggaca caaatgattg ctaaaggttt ggactttcca gaagtgacat tagttggggt
    21481 gttgaatgct gatacctccc tgaatttacc ggattttcgt tctagtgagc atacattcca
    21541 attgcttacc caagtttcag gacgagcagg gcgagcggaa aaagcaggcg aagtcgtgat
    21601 ccaaacattc aatcctcaac attacgccat agaattggct aaaaaacaaa attatgagca
    21661 gttttatcag caggaaatgc atgtacggca tcgtggaggc tatcctcctt attactttac
    21721 tgtgaaaata accgccagcc atcctgaaga acaagtagcg gcaaagaaaa tctttcagat
    21781 tgccaatcag ctaaaagaag tgttaagccc tcaaagcttg ctattagggc caacgcccag
    21841 catgattttg agagtgaaaa accgatatta ttatcaacta atcatcaaat ataaacatga
    21901 accgaatctt cctcatgttt tggatgaaat tttgaatgga agccagaaag aacagcgaca
    21961 aggtcttttt gtggcaattg acaacgaacc attaaacttt atttaagaaa gggccaaatt
    22021 atgcgctatc ctatattgat ccaccctaat gataaattaa aaagaactgc tcagccaatc
    22081 gatgtaatca cggacgagac aatcgcttta ttagacaatt tatatgagac aatgattgcg
    22141 aatgacggaa tagggatcgc tgctcctcaa gtaggacaaa acaagagaat tgctgtgatc
    22201 gaagtagatg aaggcgagaa atttgaactg attaacccag aaatcatcga agcaaaaggt
    22261 gaaagccttg acgtagaagg ttgtctcagt attccgcatg tctatggaac ggtcaagcga
    22321 gcagatgaag tgaccgtgcg ttattatgat cgagacggag aagagataga agtgacggct
    22381 tttggctatt tagcccgtgc cttccagcat gaaatcgatc acttagacgg tatcttgttc
    22441 attgagaaaa tgatacagca gatcccggaa gaagaacttg aagaatatat ggaggaacat
    22501 cttgatgact aagctagtat ttatgggcac acctgctttt tctgtgccga ttcttgaagg
    22561 gctgatagaa tcaggttacg agatccaggc agtggtaaca cagcccgacc gtccagttgg
    22621 gcggaaacga gtaattacgc cgacaccggt aaaaaaagcg gctctgaagc acggtatccg
    22681 tgtgctgcag ccagagaaaa tctccggttc gccagaaatg gaagaaatca ttgaattggt
    22741 gcctgatgtg atcgtcactg cggcatttgg ccaattcttg cctgaaaagt tattgcaagt
    22801 accaaaatta ggggcaatca atgtccatgc ttctttactt cctaaatatc ggggaggtgc
    22861 gcctgttcat tatgccatca tgaatgggga gaaagaaact ggtgtaacga tcatggaaat
    22921 gatcaaaaaa atggatgcag gcggaatcta tgcgcaagaa agcataccaa tcactaaaca
    22981 agatgatgta gggacgatgt ttgaaaagct tagcctttta ggaagaaagc tattgctaga
    23041 aacattgccc aatattttgg atggacaaaa accagtacct caagacgagt cagaagcaac
    23101 tttttcgcca aacatcacac gggaacaaga agcaatcgat tggcataaaa ctgcagaaga
    23161 aattgacaac caagtacgcg gaatgcgtcc ttggccaatt gcattcacga cctatgaggg
    23221 catacgctgg aaattattat cggttgaagt attggcagaa actacagaca aagcacctgg
    23281 tacgatcatc cggaaagaca aaaaaaatct atggattgct tgcggtaaac agaccgtctt
    23341 agccatcaat gaattacagc cagctggtaa aggaaaacag gccgtacatg atttccttaa
    23401 cggaagcggt cagcaagttc agattggtca gcaggtgaaa taatgaaaaa aatccctaaa
    23461 aaaatgaaaa aatcagtacg atatatggca ttactgacat tagagcgagt gaacaagggt
    23521 ggcgcctact cgaatctttt gttgaatgag atgatcaaac aaagcgagct aaatcaaaaa
    23581 gatgtgggtt tatttacaga attgatctat ggtacgatta gccatcagcg actattggaa
    23641 ttttacgttg caccattgat cgctaaagca aaaaaagtgg atgactgggt caagatgctt
    23701 ctttatcttt ctgtatacca attagaattt ttagataaag tccctgcaca tgctatttta
    23761 aacgaggcgg tcgaaatcgc aaaagtaaaa ggaaatcctg ggacagggaa atttgtaaat
    23821 ggtgtcttga ggaattatca gcgtcaagga gcaccagatc tgcaaggcat tgctgatcct
    23881 atcgaaagac tttctgtcaa aatcagcctg cctctatgga tgacgaaacg actggtggat
    23941 caaatcggat acgaagaaac tgaaaagctc ggtttatcgc tatatcagcc tagccatgct
    24001 agtgcccgag tagatacacg ccgactttcc agagatcagg ccatcactgt tttgcaagaa
    24061 gaagagatca cagctgaaaa aagccaagtc agtccatacg gtatagtagc gaaaaaaggg
    24121 catttagctt ctagctcatt gttccacgat ggcgtcatga cgatccaaga tgaaagttcg
    24181 atgcttgtag cgccgtctat gcaaatcgag cctgaacatc atgtcttaga tgcctgtgca
    24241 gcacccggag gtaagacgac gcatattgct tcttttttag atccgtcatg cggtggagaa
    24301 gtggtcgctt tagatgtaca cgaacacaaa gtcaaactca ttgaagataa tgcagcacgt
    24361 ctaggtgtct cttctgctgt ctacgctcaa aagatggatg ctagagaagt cagtaaacag
    24421 ttcgaagcag agcagtttga tcggatttta gtagacgcgc cttgttcagg tcttgggctg
    24481 cttcggcgaa aaccagatat tcgttataaa aaacagccgg atgaattagc gaatttacca
    24541 aaaatccaat tggaaatttt agaaagctgc gcaccaactt taaagtcttc cggtattctg
    24601 acttatagca cttgtacgat cttaaaagaa gaaaaccaag acgttgtttc tgctttcttg
    24661 gaaagacacc ctgattttga gaggattgat gttttgacca atcctatgct acaatcatca
    24721 atcgaagaga aaatgttgac tttgtatccg catcagtttt acacagatgg atttttcatt
    24781 tgttgtttgc gcaaaaagta atgaggtgaa aaaatgcaga ttgaatatca atcagatgta
    24841 ggaagacgac gtaacacaaa tcaagactat gccagtgtat ttaccagccg ttcaggtctc
    24901 aagcttgctg tattagcaga cgggatgggt ggtcatcgtg ccggagacat tgccagtcag
    24961 atggcggtaa cgaatctagg cgaagcttgg gaaaaacaag aagctgttga cgatgaaaaa
    25021 atcgcccaat ggttcattca atcgattcaa gaagaaaata tgctgattta tcagcgtgga
    25081 caagaacagc cggaatataa cggtatggga acaacaatcg tagcggccgc tctgtcagat
    25141 gactggttca cgattgccaa tgttggggat agcagagctt atttgattcg tgaagataaa
    25201 atcatgcaat tgacggagga tcattcttta gtcaatgaac tagtcaaatc aggagaaatc
    25261 agcgaagaaa tggcagtgaa ccatccaagg aaaaatattt taacacgttc tgtcggcatg
    25321 cctggaacag tcgaagtaga tgtttctact catctttggc aactaaaaga tcgactgctg
    25381 ttatgttcag atggcttgac gaacatgctg tctgaagaag aaataggaac gatcatcaat
    25441 caagaaggcc cattgtctca taaagtaact gagctgattg atcgtgccaa tgaagcaggc
    25501 ggagcggata atatcaccgt tcttcttatc gaatataagg aggacagtgt atgatcgagt
    25561 tgggcaaaaa attaaatggc cgttatcaaa tcactggaaa tatcggtagc ggtggtatgg
    25621 caaatgtatt tttggcgcac gatttaatct tggatcgtga tgtagcagtt aaagtcttac
    25681 gatttgactt tcaaaatgac cagactgcga ttcgacgatt tcagagagaa gctttagcag
    25741 caacagaact cgttcatcca aacattgtta gtgtttacga tgttggtgaa gaagacaaca
    25801 tgcagtatct ggtgatggaa tacgtcaaag gaatggactt gaaacgatat atccaaacac
    25861 attatcctgt tccttatgaa acagcagtaa atattatgca gcagatcctt tctgctattt
    25921 ctttagctca taaccatcaa atcatccacc gagatttgaa accccaaaac gtactgatcg
    25981 ataatgaagg tgttgtaaaa atcacggatt tcgggattgc tatcgcttta tctgaaacat
    26041 cgatcacgca gacgaatacg atgctaggat ctgttcatta tctgtcccct gagcaggcca
    26101 gaggaagtat ggcaacgaag cagtcagata tttatgcttt agggatcatc ctttatgaga
    26161 tgttgacggg tagcgtaccg tttgatggtg aatctgctgt aacaattgct ttaaaacatt
    26221 ttcaagatga tcttccatct ataaaagcac ttgatccaaa tgtgccacaa gcactggaaa
    26281 acgtgatttt acgggcaacg gctaaagaac ctgcagaccg ttataagtca gccgaggaaa
    26341 tgtctgatga tctttctaca gtattatcac cagctcgtgc aaacgaagaa aaatggcagc
    26401 ctcatgtgat ggataatgaa acaaaagtta tcacacccct tacggaagat gcaccaatgc
    26461 ctgattcatt caaatctatg cctctgccaa aagacaagcc agaaaacgaa accttaccag
    26521 aggaggaaga agttctgcct gatcctaaaa agaaaggaca gaaaaaatgg tggctgatcc
    26581 tattagctat tcttgctgtt ttaggaatcg gaacagctgt attctttgct tctggcgggc
    26641 gaggagaagt tgttgttcct gatgtttctg atctttctga agcttctgct agagaaacac
    26701 tcgcaaaagc gaaattagaa gtagcaacaa aaacagaaga aattgcagat gacaaaatcg
    26761 aagaaggtaa tgtagtgaag acagacccgg cagcgggaac gacagtcaag caaaaacgcg
    26821 aagtcactct gtatattagt agcggcacga aaaaaatcaa attagatgat tacagtggta
    26881 aaagttatga agatgccgaa gcaagtttga aagaattagg gttttctgct gacttgattc
    26941 aaaaagaaga ggaattcagt tcagaggtag aaagcggaat gatcatttcg caatctccat
    27001 cagaagatac agaagtcgat cctaaaacag acacgattac tttcacagtc agcaaaggac
    27061 cagaaacagt gactttgagt agtcatgtag gagaaaacta cgaaactgtt cgtaattggc
    27121 ttgttacaca aggattcaat atcaataata ttagtgctac ttatgactat agcgacactg
    27181 taccatcagg ttcgatcatc agtcaggatc cagcctcggg tgaagttgta gcagaagaaa
    27241 cttacatcaa ttttgtcgta agccaaggaa cagagcctaa attgaatgat atctccggct
    27301 atacaaaaag cgaagcacaa agttatttag ctagtgtcgg tgcagaatat atcgggcatg
    27361 agacttccga atattcaaac acggtggata aagataaagt gattcgtaca gtaccagggg
    27421 caggaacgac aattaccaaa ggaatggttg tgaacgtaat ttactctaaa ggaccagatc
    27481 cagcgtcttc tagttctgaa aaagactctt cagacagttc tagcagctca agtagctcca
    27541 gtagcacaag caactcttca aaagaagaat cttccagctc cacaagttca tcagctagca
    27601 cctcagaaag ttcaaaataa gtgctgaata aaatatatca gaagggaatg cgacattgat
    27661 ttgttgcatt ctcttttttg tgtttctatc tcttttgatc aattttcaag ccatatactt
    27721 tgagaagttt gcatggaata acacctactt tcctcttcgt atggtaaaat aaacaaaaac
    27781 ggaggtggca cttatcaaag gacaaatcag aaaagcaatc agcggttttt actatgtata
    27841 cgcaaacgga gagacctatc aaacaagagg aagaggaaat ttccggaatc ggaaaatcac
    27901 gccgcttgtc ggagatcagg tgatctttga gagtgaaaat caaacagacg gctatcttct
    27961 ggaaatcttg ccgagaaaaa atcaattagt tcgcccacca gtcacaaatg ttgatcttgg
    28021 aatcattgtc acaagtatcg tagagcctgc attttcttat aatctacttg accgctttct
    28081 agttaccttg gaatacgagt cgatcgaacc gattattttt ttgacaaaga ccgacttgtc
    28141 agacgaccca gaaaaaatcg aagagatcaa gcaaacctat actaaaatag gctatccggt
    28201 gattgtaccc aatcatacgg gtgatacgac agaactgatc cgttacttcc cagaacggct
    28261 atcggtcttt atgggtcagt ctggagcagg aaaatcgacg ctactgaatc aaatatcacc
    28321 ggaactgaat ttagcaacag gagagatttc tgatgcactt ggaagaggac ggcatacaac
    28381 acgtcatgtg gaactgattc ctatctatgg cgggttagta gcagatacac caggatttag
    28441 ctcgattgac tttttgacaa ttgaagcagg agaactgccg agacaatttc ctgaatttgt
    28501 agaagcttct gtccattgca aatttagaga gtgcatgcat gctaaagaac ctggctgcga
    28561 agtaaaacat agagtagaaa caggtggtat cgcacagaca cgttacgaca attatttgca
    28621 attcttacaa gaaatcgaaa aaagaaaacc aatgtatgca aaaaaagaag gtaaacgaaa
    28681 ataggaggaa acaagaatga agatagcacc atccattttg agtgcagatt ttgccaattt
    28741 acaaagagac attgagttag tggaaaaagg aggcgcagat tacatccacg tagatgtaat
    28801 ggatggccga tttgtcccaa atatcacatt agggccaaat attgttcagg caatccgtcc
    28861 tgttactaaa ttaccgttag acgtccatct gatgatcgtt gatccagaaa actacattcc
    28921 agcatttgca aaagctggag cagatatcat tactgtccac gtcgaagcaa ctcctcatat
    28981 tcatcgggca cttcaaatga caaaaagctt aggagttaaa gctggagtag ttatcaatcc
    29041 aggaacacca gtctccatga tcaaacatgt tttgcctttg gctgatcaag tactagtcat
    29101 gactgtcaat cctgggttcg gcggacaaag ctttattgaa gaaacagtag aaaaaattac
    29161 ggaactcgct gatctacgta gagagaacaa ttggacttat gcaatcgaag tagatggcgg
    29221 aatcgttcca gaaacagcaa aaatctgtaa agatgcaggg gcggatgtct tcgttgcagg
    29281 atcatatatc tatggagccg aaaatccagt cggacaaatc gaacgattaa aagaggcatt
    29341 agactaaatg aaaattttat tagctgctgg aggtaatccg caaaactggc cgcagtctct
    29401 gctgtccaaa tacgatgcct atgtcgggat agaccgtggt gcgctgtacc tgcttcaagc
    29461 aggctaccca gtagatctgg cagtgggtga ttttgattca ttgtcgaacg aagagcagaa
    29521 ggaagtattt gcaaaagcca aagagtatac ccgttcccct gcagaaaaag acgatacaga
    29581 tacacagtta ggtatcttaa aaacatttga acgttacccg caagcggaag tcactttgat
    29641 tggagctacg ggcggaagat tggatcatct tttagccaat ctctggttag gtatggaacc
    29701 tcgcttcaaa ccctttgccc atcagcttat tatcagagat tgtcaaaatt atttgtctta
    29761 ttatttgcct ggagagcata tagtaagaca gataccagat atgaagtatt tagcttattg
    29821 ctgcttgact cccgtctcga atcttacatt aaaaggcagc aagtatgtat tggaaaataa
    29881 agaagtagaa tatccgacat cttatgcaag taacgaattt ctttcagata ccgcagaatt
    29941 ttctttttca gatggtatga ttgcagttat tcaaagcaaa gattgatcca taaacaaata
    30001 aaaaaatttg tctttcaggc taaaaatgac agaatctatt atttgctaaa aaaaagctta
    30061 agaggagcaa cttacctttt gtttcacggc atttagtgat aaaattgaac acaagagtct
    30121 aattaattcg aggtgtcttt gtgaagaagt tcaatcctaa taaaaatatt attattacct
    30181 tggttattgt tatcatcgtg gtgacgatac taagtgtgac cgctgcgcag cgagcgaatg
    30241 aaggaaagcc caatttcttc caatccatcg ttaatgacag tgttgcaatg gttgataaaa
    30301 caatctctgc cccagtacgg tgggtacgaa atggagtaga ttctgtccat aatctttttt
    30361 caacttatgc cgaaaatgag cggctaaaag aaaaaatcga tagctatgat gacgtttcac
    30421 agcaaaacaa gaattacgag agagaaatcg aagcgttgaa aaaagaactt gagttgaatc
    30481 agacgctgac tagttacgaa aaagtaacgg caaatgtcat tactcgttcg cctgacagtt
    30541 ggcaggacat gctgatcgta gacaagggtt ctaaagacgg tatcgaagca aatatggcag
    30601 tcatgtcgca aaaaggattg attggccgag tgatcgaggt caatacggct tcgtctaaaa
    30661 tcgaattact gtcatcctct aatgaaagct ccaatcattt tccagtacgg gtatcttcgg
    30721 ctaatggcga agcgtttggt ttgcttaaaa actatgatga aaagctccat gccttagtgg
    30781 tgacccaatt aactggtgat acggatatca aagaagggga tgttgtccag acatccggtc
    30841 ttggagggaa ttctccagct aacttgccga tcggtacggt tattaaaacg aaaccagata
    30901 gttatgggct ggatcgggaa gtttatgtga aaccttatgc agaaatgtat gacgtgtcag
    30961 ttgtgacgat tgtccaacgg ctagcggagg attagtccat gttgaagaaa gaaacaatga
    31021 agtactatct gccaatcgtt ttgttctttt tgatgttgat agatggtcat ttaacaagaa
    31081 tgctagggga gtggtcgaaa ggcacctata tgtcaaatgc ccactttctg atattggcat
    31141 tattatgttg cagtatggcg tttgaaaaac gttatttact gattaccacg attgttctcg
    31201 gggctatcta tgacgcttac tatattggcg ttatcggtat ctatgcagta gctctccctt
    31261 taattgtatg gttgatgtat gtaatgaaag acgttatcca tgtcaacatc tttactgaat
    31321 ttttcagtat gatcatcttt gtcacgggtt atgaattgtt tacgatggtg gtccagttga
    31381 tttttaaatt agcagtagta aataacacgt attttattac aaggttttta ggacctacac
    31441 tgctgttgaa catgattata tttgtattat tcatttttcc ctttaagaaa ttattcagtg
    31501 atgaataatt tcttttttta tttttaggtc gaaagcgaaa aagaatcgtt gttatcttaa
    31561 gaaatgctag acttaacgcg gctttttcat gatcattttc tgaaacgaac agttttgtca
    31621 tttttaaaag ttgttttgaa acatttttgt aacataacta ttatatcgtt gacacactgc
    31681 tatggtacaa tttttatatc gttaagaaag acttaaatat atttagatat ttattaaatt
    31741 aagccagtcg gaggaatgaa gagtgaaaaa gagtttaata tcagcagtaa tggtatgttc
    31801 aatgaccttg actgccgtag catcaccgat cgctgccgcg gcggacgatt ttgattctca
    31861 gatacaacag caagatcaaa agattgcaga cttgaaaaat caacaagcag atgcacaatc
    31921 acaaatcgat gccttagaaa gccaagtttc tgaaatcaac acacaagcac aagacctatt
    31981 agcaaaacaa gatacattgc gtcaagaatc tgcacaatta gtaaaagaca ttgcagattt
    32041 acaagaacgt attgaaaaac gtgaagatac aatccaaaaa caagcacgcg aagcacaagt
    32101 aagcaacaca agctcaaact acatcgatgc tgtattgaat gctgattcat tagcagatgc
    32161 tattggacgc gttcaagcaa tgacaacgat ggtaaaagca aacaacgact tgatggaaca
    32221 acaaaaacaa gataaaaaag ctgtagaaga caaaaaagct gaaaacgatg ctaaactaaa
    32281 agaattagct gaaaaccaag ctgctttgga atctcaaaaa ggcgatcttt tatctaaaca
    32341 agctgatttg aacgtactga aaacttcatt agctgctgaa caagcaactg ctgaagacaa
    32401 gaaagctgac ttgaaccgtc aaaaagcaga agctgaagca gagcaagctc gtattcgtga
    32461 acaacaacgt ctagcagaac aagctcgtca acaagctgca caagaaaaag ctgaaaaaga
    32521 agcacgcgaa caagcagagg ctgaagcaca agctactcaa gcttcatcta ctgcacaaag
    32581 ctcagcaaca gaagaatcat cagctacaca atcttcaatg actgaagaat catcatcagc
    32641 tacacaatct tcagcaacag aagaatcaac aacacctgaa tcttcaacag aagaatcaac
    32701 agcgcctgaa tcttcagcaa cagaagaatc aacaacagcg cctgaatctt cagcaacaga
    32761 agaatcaaca acagtgcctg aatcttcagc aacagaagaa tcaacaacag tgcctgaatc
    32821 ttcaacaaca gaagaatcaa caacacctgc gcctacaaca ccatcaacag atcaaagtgt
    32881 tgatacagga aacggcacag gaagtagtac tccggctcca acgccaacac caacacctga
    32941 acaaccaaaa cctgtaacac ctgctccagc tccttctgga agtgtgaatg gtgcagcaat
    33001 cgtagcagaa gcttacaaat atattggtac tccatatgtt tggggcggta aagatccaag
    33061 tggctttgac tgctcagggt tcacacgcta tgtttacatg caagtaactg gtcgtgacat
    33121 cggtggttgg acagttcctc aagaatcagc tggaacaaaa atttcagttt ctcaagcaaa
    33181 agctggagac ttgttattct gggggtcaca aggcggaact taccacgtag cgattgcttt
    33241 aggcggagga caatatatcc atgctcctca accaggcgaa agcgtaaaag ttggttcagt
    33301 tcaatggttt gcccctgact ttgctgtcag catgtaagaa tgat
//
LOCUS       DADVXO010000015        32668 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.015, whole genome shotgun sequence.
ACCESSION   DADVXO010000015 DADVXO010000000
VERSION     DADVXO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.015"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            262..951
                     /locus_tag="KB950_000625"
     CDS             262..951
                     /locus_tag="KB950_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6856864.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            938..2146
                     /locus_tag="KB950_000626"
     CDS             938..2146
                     /locus_tag="KB950_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6856865.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2440..3711
                     /gene="serS"
                     /locus_tag="KB950_000627"
     CDS             2440..3711
                     /gene="serS"
                     /locus_tag="KB950_000627"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBB6856866.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4260..5744)
                     /gene="guaB"
                     /locus_tag="KB950_000628"
     CDS             complement(4260..5744)
                     /gene="guaB"
                     /locus_tag="KB950_000628"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBB6856867.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6249..6944)
                     /locus_tag="KB950_000629"
     CDS             complement(6249..6944)
                     /locus_tag="KB950_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBB6856868.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7034..8134)
                     /gene="ychF"
                     /locus_tag="KB950_000630"
     CDS             complement(7034..8134)
                     /gene="ychF"
                     /locus_tag="KB950_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBB6856869.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8143..8334)
                     /locus_tag="KB950_000631"
     CDS             complement(8143..8334)
                     /locus_tag="KB950_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBB6856870.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8355..9245)
                     /locus_tag="KB950_000632"
     CDS             complement(8355..9245)
                     /locus_tag="KB950_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBB6856871.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9232..9999)
                     /locus_tag="KB950_000633"
     CDS             complement(9232..9999)
                     /locus_tag="KB950_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6856872.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10011..10724)
                     /gene="rsmG"
                     /locus_tag="KB950_000634"
     CDS             complement(10011..10724)
                     /gene="rsmG"
                     /locus_tag="KB950_000634"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBB6856873.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10956..12857)
                     /gene="mnmG"
                     /locus_tag="KB950_000635"
     CDS             complement(10956..12857)
                     /gene="mnmG"
                     /locus_tag="KB950_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBB6856874.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12874..14271)
                     /gene="mnmE"
                     /locus_tag="KB950_000636"
     CDS             complement(12874..14271)
                     /gene="mnmE"
                     /locus_tag="KB950_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBB6856875.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14570..14953
                     /locus_tag="KB950_000637"
     CDS             14570..14953
                     /locus_tag="KB950_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856876.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15121..15879)
                     /locus_tag="KB950_000638"
     CDS             complement(15121..15879)
                     /locus_tag="KB950_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBB6856877.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15897..16715)
                     /locus_tag="KB950_000639"
     CDS             complement(15897..16715)
                     /locus_tag="KB950_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBB6856878.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16712..17068)
                     /gene="rnpA"
                     /locus_tag="KB950_000640"
     CDS             complement(16712..17068)
                     /gene="rnpA"
                     /locus_tag="KB950_000640"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBB6856879.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17186..17320)
                     /gene="rpmH"
                     /locus_tag="KB950_000641"
     CDS             complement(17186..17320)
                     /gene="rpmH"
                     /locus_tag="KB950_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBB6856880.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17843..19177
                     /gene="dnaA"
                     /locus_tag="KB950_000642"
     CDS             17843..19177
                     /gene="dnaA"
                     /locus_tag="KB950_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBB6856881.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19375..20505
                     /gene="dnaN"
                     /locus_tag="KB950_000643"
     CDS             19375..20505
                     /gene="dnaN"
                     /locus_tag="KB950_000643"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBB6856882.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20734..20976
                     /gene="yaaA"
                     /locus_tag="KB950_000644"
     CDS             20734..20976
                     /gene="yaaA"
                     /locus_tag="KB950_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBB6856883.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20963..22087
                     /gene="recF"
                     /locus_tag="KB950_000645"
     CDS             20963..22087
                     /gene="recF"
                     /locus_tag="KB950_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBB6856884.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22084..24030
                     /gene="gyrB"
                     /locus_tag="KB950_000646"
     CDS             22084..24030
                     /gene="gyrB"
                     /locus_tag="KB950_000646"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBB6856885.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24050..26521
                     /gene="gyrA"
                     /locus_tag="KB950_000647"
     CDS             24050..26521
                     /gene="gyrA"
                     /locus_tag="KB950_000647"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBB6856886.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26757..27056
                     /locus_tag="KB950_000648"
     CDS             26757..27056
                     /locus_tag="KB950_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBB6856887.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27106..27618
                     /gene="ssb"
                     /locus_tag="KB950_000649"
     CDS             27106..27618
                     /gene="ssb"
                     /locus_tag="KB950_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBB6856888.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27644..27880
                     /locus_tag="KB950_000650"
     CDS             27644..27880
                     /locus_tag="KB950_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBB6856889.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28023..29996
                     /locus_tag="KB950_000651"
     CDS             28023..29996
                     /locus_tag="KB950_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBB6856890.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30002..30454
                     /gene="rplI"
                     /locus_tag="KB950_000652"
     CDS             30002..30454
                     /gene="rplI"
                     /locus_tag="KB950_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBB6856891.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30728..32095
                     /gene="dnaB"
                     /locus_tag="KB950_000653"
     CDS             30728..32095
                     /gene="dnaB"
                     /locus_tag="KB950_000653"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBB6856892.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         9929 a         6393 c         6454 g         9892 t
ORIGIN      
        1 aagtcaataa gaaaatgctt atttatcaac atttctagct tacatgatca ttggttagtt
       61 ctggtccttt ttcgttcgtc tttataactc cgagagacgt aaaatccttt tgttttcagt
      121 ttaatgttca tgttcttgtc ttattcacac caactagatg tctcaccttc taaaattttc
      181 cttaacaaag tgctgctttt atctatttct gctttttttt gctagaataa tagtaacaat
      241 atgactctgg aggatttatt tatgaaaatt ttgattgctg acgatgataa agaaatcgtt
      301 gaattattaa gtatctatgt tcacaatgag gggtatgaag cagtaaaggc ttatgatggg
      361 aaagaagcat tatctaaaat cagaacaatc cctgatattg aattacttat tttagatatc
      421 atgatgcctg agatggatgg gatgcaagta gtgaaagagc tacgaaaaga atcccaaata
      481 ccaatcatta tgctgacagc aaaaactact gatatggata agatcaaagg actggtcgct
      541 ggtgcagatg attatgtgac caagccattt aatccgttag aagtaatggc tagagtaaag
      601 tctttattac ggcgtacaaa aatgcagatt gccccagatc agcctgatgt attagaagta
      661 aatgcattaa tcatcaataa agactctcat gaagttaaaa caatcgatgg gaaagaaatc
      721 cagttaacag ctttggaatt cggtatcctc tacttgcttg ctagtcatcc taatcgagta
      781 tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa
      841 actgtcatgg ttcacgttag tcatttaaga gataagattg aagaagcaac tggtggtgaa
      901 aaagtcattc aaaccgtttg gggagtgggc tataaaattg acgcaagata gtaaacgagc
      961 aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga
     1021 aggaatcatc acgattatcc ttttgcttct attaaatgtt tctattctgg ttgttatcag
     1081 ttcggtgatc aacagttcac cttctttgac gaatgcgatt tgggattcaa agaatatttt
     1141 tgcagaacga ttgaacactg atttgttctg gaacggccgg aattttatta ttcccttttt
     1201 ctttctatta gatattggtg ttttgtattg gcgcttgatt cgaagatatc gccaaatgca
     1261 attgcggcat atcatcagcg aacttcacta catcgcaaac ggaaattatg atcatcgtat
     1321 cccttttgaa ctgagtggtg acttaagccg agtggtaaca agtatcaatg gattggtgga
     1381 tagtaccgta gcagcgattg aagacgaacg taaaatcgaa aaatcaaaag atgaattgat
     1441 cacgaatgtc agtcatgata tccgtacccc actcacgtcc atcattggat acctaggact
     1501 gatagaagat ggccagtatc actcagaaga agatctttta aagtatacgc atactgctta
     1561 tatcaaagca aagcagatga aatctttagt ggacgactta tttgaataca ctaaggtacg
     1621 ccagcctgct gtcccggtga atttttctgc ctttgacatg atccagttga tcgaacagtt
     1681 agcagctgat tttgagttgg aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa
     1741 agtagactcc cttatcatgg atggcgatac cgaaaaactt gtacgtgtct tcaataatct
     1801 tttgacgaat gcgttgaaat acggaaaagg agcaacgaag atcgtaatcg aagtagaacg
     1861 tatcggttca gaagttgttg caacagtcaa aaataacgga gcaatgatcc cacaacaggc
     1921 aatcgataat ctattcgacc gtttttatcg tgtagaagaa tctcgttcac aagcaactgg
     1981 aggaactgga cttggtttag caatcgctca aagcattgtg gctttgcacg gcggatacat
     2041 ttatgcaaaa tcagataagc aatggacttc tttcatcatg caccttccaa tcaagaaaaa
     2101 tgaaaaatta cctattgata cacaggaagt tattgacgaa agctgaaata ttcgctattc
     2161 ttattacaat tattagaata aatgaaaaac acagaaccca gtacacttta ttctttttca
     2221 gagagctgat ggcaggtgta aatcagcaaa ggataatttg gaagttccag ttcgtttacg
     2281 tgccctttcg aaataagaaa ggtcggttca acagccgtta tgtgttcaac aagtgcagct
     2341 gacgctgaac ttgggtggta ccgcgaattt cagactgatt cgtcccaaga ataatgggat
     2401 ggatcagtct ttttttattt tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg
     2461 cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca
     2521 ttagctgaat tcatggaact agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa
     2581 ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa
     2641 gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat
     2701 actgaaattg ctgaagtgga tgaaaaattg aaacaaattg caactacttt gccaaacctt
     2761 ccccatgatt ctgtgccagt tggaacagat gaagaggaca atgtagaaat ccgtcgttgg
     2821 agcgaaccac gtacatttgc ttttgaacca aaaccacact gggaaattgc agaaaatctt
     2881 gatattttag attttgaacg tggcgcaaaa gtagccggta gccgttttgt ctattacaaa
     2941 ggattaggtg ctcgattaga acgtgctgtc tacaacttca tgcttgatca gcatatttac
     3001 gaacacggct atactgaaat gatcactcct tatatcgtca acagtaaagc gatgtttggt
     3061 acgggacaat ttccaaaatt caaagaagat gttttccaat tggagaatac tgatctaaca
     3121 ttaatcccaa cagctgaagt accattaact aactattata atgatgaaat tttagatggt
     3181 gcagatttac ctatttattt cactgcacta agccctgctt ttcgttctga agccggaagt
     3241 gctggtcggg atacacgagg actgattcgt cttcaccaat tcaataaagt agaaatggtc
     3301 aaattttcgg atgtagagca ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa
     3361 gatatcttga aaaaattgca tttgccttat cgtgtgatgg ccttatcaac tggagacatg
     3421 ggcttttctg cagctaaaac ttatgactta gaagtatgga tacctgcaca agatacttat
     3481 cgtgaaatca gttcttgttc taactgtgaa gatttccaag cacgccgcgc aatgatccgt
     3541 tatcgcgata atgatggaaa aatccactat actcatactc tgaatggttc aggacttgca
     3601 gttggccgta cagtagctgc cattttggaa aactaccaaa atgaagatgg ttctgtcaca
     3661 gttccagaag cgctcgttcc ttatatgggg aatctgaaag ttattaaata aagaggctgt
     3721 gaaaaagccg cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt
     3781 ttgcataaat tggacttaat accgagaaac tggcttttga acaccattta tcgagaggtt
     3841 gtgaaatcag cgaattaacc tgagtattag agaaaaaaat ggaaaaatag tccctcatat
     3901 tttaccgttt gttgaactaa taccgagggt cagctgattg aacaccgttt agtggagagg
     3961 tcgtaagcct accccccagc tcctagttac aaggacaaat tcaaaaaaca gctcttcata
     4021 ttttatgaat gttgtacttg tgattcggag gtggcaaggg aacactgttt attcaaaaag
     4081 aggctgggac agaagcgttt aactccaaga aataagaaga aattcacgaa aattgctttt
     4141 caaatttttg tgaatttctt cttatttccg aaggagttgc ttctacttcc gccgtttata
     4201 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaatgtttt
     4261 taagattcaa ctgaatagtt tggtgcttct tttgtgattt gtacatcatg agggtgtgat
     4321 tcttttaatc cactgccgct catttggata aattgcgctt catcacgtaa ttgctgtaga
     4381 ttggctgccc caacataacc cattcctgaa cgcaaaccgc cgatcatttg gaaaataata
     4441 tctgcaacac ttcctttata agcaacccga ccttcgattc cttctggtac taatttattt
     4501 gcttcattga cactaccttg gaagtaacgg tcttttgaac ctttttccat cgcgcctaat
     4561 gagcccatac ctcggtatgt tttgaatcga cgtccttgga agatttcgaa ttcacctggt
     4621 gattcatctg tccctgccaa catacttcct agcatcactg catgtccgcc ggcagctaat
     4681 gctttgacga tgtcaccaga atatttgatc cctccgtctg caataatcgc tttgccatat
     4741 tgacgagcaa ctgatgctgc atcataaata gctgtgagct gaggtacacc tacaccggct
     4801 accacacgtg tcgtacaaat cgatcctggt ccgataccaa cttttacgac atctacacct
     4861 gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt ttcctgcaat caatgttgca
     4921 tctgcaaaag ttgaacggat ttcttgaatt tttcggatca cacctgcact atgtccatga
     4981 gctgtatcga tgatgattgc atctgcccct gcttctaata aagcattcgc acgctcaaat
     5041 gtatcgcttg ttacaccaac ggctgctgct acaagcaatc gaccatgttc atcttttgct
     5101 gcatttggaa attcgatcac tttctcgatg tctttgatcg tgatcaatcc gcttaaacga
     5161 ccttcgttat ctacgattgg taatttttcg attttatgtt tttgcaaaat cttttctgca
     5221 tcttttagag aggtacctac tggggcagtc actaagttgt cttttgtcat cacatcagca
     5281 atcggcatgt gataatctgt tacaaaacgc atgtcgcggt ttgtaattat ccctaccaat
     5341 tttctgtttt caagtgtttc aacgattggc acaccactga tacgatattt gctcatcaat
     5401 tcttctgcgt ctgctacaag atgctgtggc gttaagaaga acggatcaat gattacgcca
     5461 ctttcagaac gtttgacttt tcttacttca tccgcttgct ggctgatcgt catgttttta
     5521 tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg ccatcttgct gtcggtaaca
     5581 gtatccatac ttgcactgat gattggaata ttcaacttaa tatttttagc taattgtacg
     5641 ctcatatcca cttcgtttgg taacacgtga ctttctgctg gtatcaataa gacatcatca
     5701 aaagtaaatc cttttttcgt gaactttgtt tcccagttgg acatttttag taccactcct
     5761 ctaatttagt ttatctatta aaataattga ttttttcatt taagtcaatg atctacttgg
     5821 aatcaagtta gctttctcat agaataccaa tataaagcgt ttacatctgt gtgtattaaa
     5881 agagattgta aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
     5941 ctcatgtttt tgaattggtt gggtttatta ccgcagatgc aaacttttga acaccgttta
     6001 gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
     6061 gctttccaca tttttgcata aattggactt aataccgaga aactggcttt tgaacaccgt
     6121 ttatcgtaaa aagggactgt gacaagactt ttgtcacagt cccttactat tatttaacta
     6181 tggataaaca aaatgaggtt ctttactacc cctagatatc tagcaaaata gctgatcttt
     6241 ttgctagttt atcttcttgg gacggataaa cttccaataa tcccgtattt tttcttcaag
     6301 tagtaacgga ttgctaaagc agttgccccg ataatgataa ctactactgg atctaatact
     6361 ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca gtacgaccca aagaagtgtc
     6421 gttccaccta ggataagcaa tgtcttaccc attcctggtt tcttgcttcg atctgctccg
     6481 ggatattcat attgatagat gtatttgtac atcatataaa agacccagcc accgattgca
     6541 gaacctagaa cgagtgtaga aaagccataa gctggcgttg ttccacgtga aaacaatccg
     6601 gtaatcgcaa tcatgatact taatagacca agcaacagca acgtattatc taaccacatc
     6661 aataaagggg tggatgaatt ttttacttct gctggcttat tgatgattgc ttccgttctt
     6721 tcagagactg tcccaaacaa ttgacgggct gttcttccgc ctttttgttc tgcaactaaa
     6781 gtaggcagaa tgtcatgaag ggcttttact ttttcttctt cagacaaatt cgctgcttct
     6841 aaagattttt tcaaatcaaa aatgtactgt tgatttcttt tggttaattt tgtttctaaa
     6901 ctattgtttt cttttacgat tgctcgtaat tgttctggtt ccattatcta atagtcctcc
     6961 ttagccgttc cttttctcaa cggtcgtcac aaaagacatt ctaccatatt ctacagctag
     7021 tttgatagcc cttttacaca ttaaagcgga ataacattac gtcgccgtct tgtacgacat
     7081 aatcttttcc ttctaatcga acacgtccag cttcttttgc tgcatgcatg tttccgtaat
     7141 gatccaagtc ttcaaaagag acagtttctg cacggataaa tccgcgttcg aaatctgaat
     7201 ggatgattcc tgctgcttgt ggtgctttga ttcctttttt gaatgtccaa gcacgtactt
     7261 cttgttcccc agcagtaaag taagtagcta agcctaacaa atcataagca gcacggatca
     7321 actgatccaa acctgattct tcgattccta aagcttctaa aaattccgct ttgtcttctt
     7381 catctaattc tgcgatttct tcttctgcac gagcactgac cacgatcact tctgcatttt
     7441 cttctgcagc aaagttgcgc acttcttgta cataatgatt cgcgtctgca tctgctacgt
     7501 catcttctga gacattggct acataaagaa ttggttttgt cgtcaataaa aatagtgatt
     7561 taacgatttt ttgttcttct tcagtaaatt cgatcgttcg ggcagatttt ccttcttcca
     7621 gtacaggttt gattttatcc agtacagcta gttctgccac tgcatcttta tctttggttt
     7681 tagcaacttt tgctacacga gtatagcgtt tgttgataga ttctaagtct gctaacacta
     7741 gttccaagtt gatcgtatca atatcttcta gtggatcaac acgtccttct acgtgagtaa
     7801 tattatcatc atcaaaacag cgaacaacgt gacaaatagc gtctacttgg cggatatggc
     7861 ttaagaattg atttcccaaa ccttctcctt tacttgcgcc ttttacgatt ccagcaatat
     7921 ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg tacaagttct gttaagcgtt
     7981 gcaaacgcca gtcaggtact tctaccatcc ctacgttggg gtcgatcgtc gcaaagggat
     8041 agtttgctgc ttctgctcct gcttttgtga tcgcattaaa caaagttgat tttcctacgt
     8101 tgggcaaccc aacaattcca gcagtcaatg ccataaattt attcactctt tctctgcatt
     8161 tttttcaatt acttttttta atttcttttc aaaatctcga cgggtcatca tgacgatatg
     8221 tccgcaattc atacatctga ttttgatatc tgcgcccatt cgaatgactt cccagcgatt
     8281 cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg tgcaagtcgt acataaaaat
     8341 agcctccttt ttgtttattc ttcatcaaat tgaatattca agatatcaag aattcgagtc
     8401 aaatcttttt gagaaagata ttcaatctcg atcttacctt tcccattttt ttcttggata
     8461 gctacacttg taccaaattt atccatcaaa cgatcttcgc tttctcggat ataatatggt
     8521 ttttcctttg ctagccttgg gatttttttc ttttcttttc catcattttg atttaactca
     8581 gaaacaagct gttccagttg gcggacagtt aaattttcag ttactgcacg attggctaat
     8641 ttcaagatca atgttttatt ttttaaacct aacagcgtac gggcttgtcc catagataaa
     8701 cgctgatctt gtaccatttc cttgacaaga tccggtaaag aaagcaagcg caaataattg
     8761 gcgatataag gtctgctctt tcctaaacga ctagctactt ctgcttgtgt tagtttcagg
     8821 tttttcatca gcatctcata tgcttctgct tcttctaacg gattcagatc ttctcgctgc
     8881 aagttttcca gcactgctac ttgcatcatc gcttcttcgt caaagtctct aacgatagca
     8941 gggatcgttt ctttttcagc tagtttggac gctcgaaaac gtcgctcccc tgcaataatc
     9001 tcatatcctt ttactgcgga acggcgtacg atgatgggtt gaaagacacc tgattgttca
     9061 atagaattag ctagttcctg caaagatact tcttcaaatg ttttacgggg ctgatacgga
     9121 tttggtcgca gctcattcaa tggtagctgg acaacggtat catcttttac atccaccgtc
     9181 tctaagtttt ctaaatcttg aaacagtgca tcgattcctc ggcctaatcc tttacttttc
     9241 ttcacgactt accacttcct ttgccaatgc ttggtagact tcagctcctc gagaacgtgg
     9301 atcgtagtcg atgatcggtt ttccatgact aggtgcttct gataaacgaa tatttcttgg
     9361 aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt acttcttcta cgacttcatt
     9421 tcccagattt gttcgtgcat catacattgt cagtaagact ccctcgatct ctaattctgg
     9481 attaaagtgt ttttggacta aacgaaccgt attcagtaat tggcttaatc cttccaatgc
     9541 gtaatactca cactgaacag gtatcaaaat cgaatcactt gctgtaaatg aattgattgt
     9601 cagatggcca agagaaggcg ggcaatcgat caaaatataa tcatactgac tgcttacttc
     9661 tgctaaagat ccttttaatc ttgattctct tgccatcatt gacgtcaatt cgatctctgc
     9721 tcctgctaat tggagagtcg caggcacaat actcaaattt tcgtgttcag tgatcaaagt
     9781 tgcctcatca attggcaatt cattgactag aacatcatag atgtctcttg tcacatcagg
     9841 ttttctgatc ccgacaccac tggtagcatt tccttgtgca tccatatcca ctagcaatac
     9901 ttttttgcca aggctagcaa gacaagcacc tagattcacc gtcgtcgttg ttttgccgac
     9961 gccacctttt tggttcgcaa cagatattat ttgtgccatc tcttctcctc ctatttgatt
    10021 ggctgcttat taggcagtcc gggctttctt gggtatttct ttggggttgg tttctttttt
    10081 tgaataacga tgatatgccg ttgatcagag acattcggca aggtaaccgc ctcttctttg
    10141 aggaatttac cacctaataa agcaatcgct tgttttgctt ctgtcaattc ctcttcactt
    10201 tttgctgctt tcaatgcata aaaatagcct tcttgtttag ccaagggcaa acacaattcc
    10261 gctaacacat tcaaacgagc aaccgcacga gcagtgacaa ggtcaaatga tgcacgaaag
    10321 tgaggatttt gcccaaatgt ttctgcccgg tcatgatata aggaagcatc gattcctaat
    10381 aatcgggtta actcagataa gaaggttatt cgtttattca gagaatcaac gatcgttatc
    10441 tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa accctgcccc cgaaccaaca
    10501 tcgcataaag ataaaggttg cgtaaagtct acttcaaatg ctaatgtaag tgaatcgtaa
    10561 aaatgcttca gatatacgtc tttttcatcc gtaatagctg taaggttcat cttttcgttc
    10621 cattcgacta atgtccgata ataaatagcg aattgattca tttgatgatc ggtaagctct
    10681 atcccttttt ctgccaagga ttggcgaaat tcttctggta tcataaatat tcctttcatt
    10741 gtgaaacagc aattgtttca cagattctct ccctacttta acgcaaaatc tctagacatg
    10801 caatccttcc atatggattc ttaaaaattt gaaaagtaag aaaagccgat agactttttc
    10861 tttctagtat gactcataaa atatacacag aagagagaac tagctttgag caatttctgc
    10921 aaaaactagt tctctcttct atttattatt acgtattacg ctggttcact gactttcgca
    10981 atcttccctt gttcgatata gaccattagg atacttaggt ctgcagggtt tacgccactg
    11041 attcggctag cttgggcaat tgtttctggt tggattttct gcaatttttg ttttgcttct
    11101 gttgctaatc cgttgattgc tgcgtagtcg atgttttctg gaatcttttt cgcttccatg
    11161 cgttttaatt tttctacttt ttccatggct tttttgatgt agccttcata tttgatttgg
    11221 atttctactt gttcgatttc ttttcgtgtc aattcttcat tttctggaat aaaccgtaga
    11281 agttctgcat aagtaatctc aggacgtctt aaaaagtcgc ttgctagtac accatctttt
    11341 aatggtgccg cattaatcgt tgctaaaaag tcttggactt cttctgttgg tttgattcgt
    11401 acattcatca aacgtttgat ttcttgttca actgcttctt ttttcttcaa ataattcttg
    11461 tattgatctt cttttactaa gcctacacgg tgacccattt ctgttaatcg caaatctgca
    11521 ttatcatgac gtaaaagcag acggtattca gcacgtgaag tcagcaaacg ataaggttcg
    11581 ttcgttcctt ttgttaccaa atcatcgatc atgacaccga tgtaaccgtc acttcgtttg
    11641 atcactaaag gttcttttcc ctgaactttc aaagctgcat tgataccggc aatcagccct
    11701 tgtccagccg cttcttcata accgcttgta ccatttgttt gtccagctgt ataaagattt
    11761 tcaacgactt tagtttctaa tgtcggacgg agctggtgtg gctctactac gtcatattcg
    11821 atagcatatc ctgtacgcat catttctact ttttctaatc cagcaataga atgaagcatg
    11881 ttgacttgaa catcctctgg taaagaagta gataaacctt gaacatagac ttcttccgta
    11941 tttaagcctt ctggttccaa aaataattga tgacgtggtt tgtctgcaaa acgcacgatc
    12001 ttatcttcga tggatggaca atagcgagcg cctacccctt caacgatccc tgtaaacatt
    12061 ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg tctcacctgt ataggtcagc
    12121 cagcaagatt cctgatctag tttataggca ctatctggtg tgctgaagct aaaatgattc
    12181 ggctctttgt caccaggctg ctcttctgta acagaatagt cgatcgtact tgattttact
    12241 cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc caagttcttt tagatgattc
    12301 gctagtccaa tagaaggttg agagttattc ggacctgatg aatatttcaa ttcaccgata
    12361 atgatttctc cgcgaagtgc tgttccggct gtaataacaa ctgctttggc ccggtagctc
    12421 gctcctgtgg acgtcactac tccatgacaa acaccatctt cgacgatcag ttcttcgacg
    12481 attccttgtc ttagcgtcaa atttggttct ttttcgatcg tatgtttcat ttctgttgaa
    12541 taagcatgtt tgtctgcttg tgctcgcaat gctcggacag ctgggccttt tcctgtgttc
    12601 aacatacgca tttggatata ggttttatcg atattcttac ccatttctcc gcctaacgca
    12661 tcgatttctc gaacgacaac gcctttagct ggtccgccta cagaaggatt acatggcatg
    12721 aatgctacca tgtccaaatt tattgtcagt aataatgttt ttgatcccat acgtgcagca
    12781 gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa cgatcacgtc atacgcatct
    12841 gcttcataat gttccaatcg tttgttcctc cttttatttt cctaaacaaa attgactgaa
    12901 taattgagtg attagctcat cttgtacact gtcgccgaca acttctccta gatagtccca
    12961 gcaacgcgtc atatctattt gaacgagatc cacaggcatt cctgaatcaa tacctgcaat
    13021 gacttcttca agtgagaggg aagctttttc taacaacgcg atatggcggg tattagaaac
    13081 ataagtagcg tctctttctc ctgtttctcc gctgaagaat aaatcagaaa tagctgattc
    13141 caaacgatct agtccgtcgt ttttagctac ggaaattgaa aaaactggtt cattttcgat
    13201 caatttcttc aatttttctt gttccagctg tgctggcaaa tctgttttat ttagaagaat
    13261 gatccgtttc aatccacttg tcgcttccaa taattgttca tcttctgctg tcagtggttc
    13321 gctttggttt agaacaagta agatcaagtc tgcttctgct aatgcttttc ggcttctttc
    13381 gaccccgatc cgttccacca catcttctgt ttcccgaatt ccagctgtat cgattagctt
    13441 gagcggtacc ccccgaacat tgacatactc ttcgattaca tctcgagtcg ttcctgcaat
    13501 atccgtaacg attgcttttt cttctcttaa caaatggttt aacaaacttg atttgccgac
    13561 atttggccgg ccgatgattg ccgtacttaa cccttcccgc agaaccttcc cttgcttgga
    13621 tgtagcaagt agcgcttgga ttctttgttg gatcatctgc gctttttcaa gcaataactt
    13681 cgtagtcaat tcttcgacat catcatattc tggataatca atatttactt ctacttgtgc
    13741 taatgtctct aaaatctctt gtctcaatga gcggatcaaa gcagacagat tgccgtctaa
    13801 ctgattaaga gctagtccca ttgctttgtc tgtttttgcc cgaatcaaat ccatcacagc
    13861 ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct cgttttgtga attcgcctgg
    13921 ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa atttgattga ctacaacgat
    13981 cccaccgtga caattgattt ctactacatc ttcccgcgta aatgtctttg gtgctctcat
    14041 cacggagacc atgacttcat cgacaagctg attgctcttg ggatcaacga tatgtccata
    14101 atggatcgta tgacttggta cttctgataa tcgtttgttt ccagattgat aaactttatc
    14161 tgctagttgc acagcttgat cgccacttaa acgaacgata ctgattgcac cttctccagg
    14221 tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta atttcagcca ttttttcact
    14281 tcctttttca ttttgcgcac aaaaaaagtg cccactccac cctttatgca caataaacgg
    14341 tgaacttcgc actttgactg cttgttcttg attaaattct ggtcatagat tatcacactt
    14401 gctataagga atcaagtgtt taaaacaatt agatgaagtt gagcctgttc tgcttccatt
    14461 accttatatt taattgtcct tggatgtctc tttgcgttct ttggtttatt cgaagagaag
    14521 atactgtata aacaaggctt tatctttctt aaaaaaaacg gtacactaaa tggtagcgct
    14581 atctaaacta tacacttttg aaaggagaaa atatgtgagc ccgttcagat ctgctgcatt
    14641 ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta tctacaggga atatcaacca
    14701 gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc ttagaagctg atttgtttga
    14761 cccatctttt cccaaagatg accttttctt aacaacgaag aggaaattaa agacgaaggt
    14821 acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt gagtatcagc aaaaaaaacg
    14881 cgacggaaag tattttctgg aactttttgc ttatggaaca gtgatccaat ttatccaaac
    14941 aacgattggt taaatgaaac agacaaaaaa agcggatcaa aactgaagac agtttcgatc
    15001 cgcttttttc tatcaaagtt agtgactact tcatcgtata ctctgataag aatgatttct
    15061 agtaaaatat acttggtttg aaaaactttg tcaacgtgat ttattcaaaa agtaatggaa
    15121 tcaataatat tttttagcag gttctactac taaataacga taaggttcct caccttccga
    15181 atgtgttttt acatgttcat ctttgcttaa tgcagcatgg atttgtttac gttcaaatgc
    15241 aggcattggt tctaagaaaa caggctgccc tgtccgtttt actttttcag ccgtgcgatc
    15301 agctaaacgc tgaatgatct cttggcgctt ttcacggtaa ttgcctacat tgactactac
    15361 agacagctta tttcttgcta aacgatggat gaagacttgt gccagatatt gcaatgcatt
    15421 cagtgttttt ccatgtttcc caatcagtat gccttgcttt tgtgtttcta aatggaaaac
    15481 aaccatatta tttgattctc tttccatttt gacaagagct ggagcatcaa gctgttttga
    15541 aatttcagta agataaactg ccaattccgt taaagctgct tcatcaccta gctcacttac
    15601 ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc tcgatctcag ctgttggttc
    15661 ttcttccaaa atttcttcta cagtctgttc tactgtttca gaaacttttt cgctgatact
    15721 tggctggatc gaaagccgtg cattctttcg acccattcct aggaaacctt tttttccttc
    15781 ttccagtatt tcgatttcga cttggtcttt ggccaaaccc aaatcagcca gacctagttg
    15841 gacagcttcg tctattgttg cagcttcata aatcggcatt agcattacct cccctattat
    15901 ttccgttttt tcttaggatt cattgctttc ttaagagcac gttctctttc gcggattctc
    15961 cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt tgttgatcag cagcgtttgt
    16021 acaacttgga aggcattgga aactacccag tataatgaaa gaccgctggc taaattgaca
    16081 cccatcacta aaatcattac aggcatcacg aaattcatga tcttcattga tgcattcgat
    16141 tcaatttgac tcatgctaga taaatacgta cttgcaaaag taaagattgc tgctaaaatc
    16201 ggcaaaataa atgttggatc cgcgttccct aagttcaacc ataagaaatg cccttgtttc
    16261 aactctggta cacgtgaaat agattgccat aacgccatca agattggcat ttgtactaat
    16321 aaaggaagac aaccagcata tggattgacg ccattttctg catataaacg ctgctgctct
    16381 tctcttaact tactttgtgt ttccggatct cttgaactat attgttgctg cagtgctttt
    16441 aatttcggtt gtaattcttg cgttttacgc atgctctttg tttggaaatg catcaacggc
    16501 aataggatca cgcggatgat cagtgtaaat aaaatgatcc ctatccctgc attaccaaat
    16561 gacaacgcct tgatcgcctc agcaaagtaa taaacaatat aacgatccca aattcctgta
    16621 ctctgtgcgc tgacttcccc agttccgcaa ccggataaga cgatgaccaa actaacgata
    16681 ccggccatca aaagtaaacg tttgtatttc ttcacttact cttttcctct ctgtcgatta
    16741 tttttgcaag ttttaagacg tggactaaat tggagcgaac ctcatcataa gtcaacccct
    16801 tcacacttgg tctagcaatc acaataaaat ccaactccgg atcaatttct tcttttatag
    16861 aataaactgc tgctcggatt tttcgtttga cttcattgcg gtttaccgcg ttgccgacct
    16921 tttttcctac agataaacca actcgaaaat gaggctgtcc cggtttttcc aaacggtaaa
    16981 cgacgaattt gcggttagca aaagaatctc ccgtatggaa cacggcttga aactctcgtt
    17041 ctttttttac tcgatacgct tttctcatta gttttctttc cttttctatc tctcacttac
    17101 gctcataatc ataccataat tacgattgtc ctttcatcat ttccctaaaa aagagtataa
    17161 aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac ttttcttcct ttgcgacgac
    17221 ggctagctaa cacacgacgt ccatttttag tactcatacg tttacggaat ccgtgaactt
    17281 tttgacgttt acgtttattt ggttggtatg ttcttttcat atattccacc tccaaaatgt
    17341 tcttatctat tttataaaca cagacatact ttgatagtat aacgacaaag tagtgatttt
    17401 gtcaatataa aatagaagat cgattgttaa aacttatcca caagattatt tttttctatg
    17461 ggaatcctat gtggaaaaag ttatgcacag ggattatcca cagataataa aaggctggtt
    17521 ttacacagaa aacacacgtg taaaaaaagt aatccacaat caggcgattt cctgtgtatt
    17581 ccgtctccta actattggta tatctgtatt ttttatccac agacgcaaga ggaaatctat
    17641 tggtttttac gagttttcca cctgtgcatt agtggtgtgg aaaacttttt ttgacaactg
    17701 tgtttttctc ttttttcttt ttgtcgattg tgtggataat tgtaattaaa aaattttttt
    17761 cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat tagtactagt tattcacttt
    17821 ttaatgaagg aggaggcctt atatggtatc cctcgatgct ttatggaatg aattaaaagc
    17881 aacataccaa aaggatttat cgcctgctag ctataataca tggatcgaaa cagcgaaccc
    17941 tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct agcaagattc ataaagaata
    18001 ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt tatatgttat caggaaatga
    18061 gattatccct cgctttatta ctggcgaaga agcagagcaa gaagaagtta tagaagaaaa
    18121 aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc atgctgaacc ctaaatatac
    18181 ctttgatacg tttgtcattg gtaaaggaaa ccagatggct catgcagcag cgctcgttgt
    18241 agcagaagat ccaggttcta tttataaccc gctcttcttt tatggaggcg tgggacttgg
    18301 taagactcac ttgatgcatg caattggtca tcaaatgctg caaagtcagc ctaatgccaa
    18361 agtaaaatat gtaagcagtg aaacttttgc taatgatttc atcaattcga tccaaaacaa
    18421 aacagcagaa gagttccgtc aagaatatcg aaatgttgat ttactgctag tggatgacat
    18481 tcaatttttt gcagaaaaag aagctacgca agaagagttt ttccatactt tcaatgcctt
    18541 gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg ttgccaaacg agattccgaa
    18601 actgcaagaa cgccttgttt ctcgctttgc atgggggctg tctgttgata ttacaccgcc
    18661 ggatcttgaa acaagaacag cgattttacg taaaaaagcg gcggctgaac gtctagagat
    18721 cccagatgac acactaagct acatcgcagg acaaattgat tctaacattc gtgaattaga
    18781 aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat agtgaagata tttcgactag
    18841 tctagctgca gacgcactga aaacgttaaa atcaggaaaa ggacatcctc aattgtcaat
    18901 cctgcagatc caagaagaag tggcaaagta ttatcacatc caattgaaag atctcaaagg
    18961 aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc gctatgtatt tggcacgtga
    19021 gctcacagat aattctttac cgaaaatcgg agctgaattc ggtggcaagg atcatacaac
    19081 ggttatccat gctcatgaaa aaatccagca gctgatggat tctagtgtca gtatgcaaaa
    19141 tgaagtatca gaaatcaaaa actttttact aaattaattt gtcgattcta tcaactgaaa
    19201 atagcatgtg gataaaagaa aaaaacgttc aaaagttata cacacttttt ccacagctgt
    19261 tttttcgttt ctctcttacg ttttttgagt tttccacaga atcaacatgc cctattactt
    19321 ttattattct ttttaactaa taataaataa acaaacattc aaaggagata catcatgaaa
    19381 gttactttaa accgagctag ctttatgcag gaattgcaaa ctgttcaacg agctatttca
    19441 agcaaaacca cgatccctat tttgacaggt gtaaaaatca cactgacaca agaaggtttg
    19501 actttgacgg ggagcaacgc tgatatatca attgaaactt ttttgtctgt tgaaaacgaa
    19561 aaagcaaata tgcaaatcga atctactggt tccattgttt tacaagcacg tttctttagc
    19621 gaaatcattc ggagacttcc tgaagaaaca tttactttag aagttttaga aaataaacaa
    19681 gtagcgatca cttctggaaa agcgaatttt atcgtaaatg gattagatgc agataactat
    19741 cctcatcttc ctgttgtcga aagccataac cagatgaaat tacctgtaca cgtattgact
    19801 aaactaatca acgaaacagt ttttgctgtc tcacaacatg agagtcgtcc aatcttgaca
    19861 ggtgtgcatt tcattttatc tgataattct ttattagctg tagctactga ttctcaccgt
    19921 ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc attttgatat tgttattcct
    19981 ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg aagaagaaat cgtcgaaatc
    20041 agcattatgg aaaaccaagt gttgttcaaa acagaaacga tgtatttcta ttcccgtttg
    20101 ctagaaggaa actatccaga taccaatcgt ttgattcctt caagttttaa tacagaggtt
    20161 gaattttctg ttcctagctt tttagcggcg atcgaacggg cttctttact ttctcatgaa
    20221 ggccgtaaca acatcgttcg tttatctatt cgtccagatg cggttgtttt atacggaaat
    20281 tcgccagaaa tcgggaaagt agaagaaagc ttgagttata cagcaagcag tggcgatcca
    20341 ttagatattt ctttcaatcc ggattatatg aaagcagcgt tgcgtgcatt tggagatatg
    20401 agcatcaaag tgaaatttat ctctgctatt cgtccattta cattggaacc aacggaagat
    20461 ggcgttcaat ttattcagct gattactcct gtacgtacaa attaacaatg attttcaatg
    20521 ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa ttttaaacag aaggccctaa
    20581 aatctctttt aaggctttct gtttattttt gtttaaatca ccttttttag attaaatcgt
    20641 cttatatgga aaaatatatg gataaatttg ttttattgcg aaaaaaaagg tataatagag
    20701 tatactttta ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg
    20761 gaattcatga cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa
    20821 gcaaaatggt atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt
    20881 ggacgcaaac tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg
    20941 gtaaaaaaag gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa
    21001 ttacgaagag ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc
    21061 gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg
    21121 gacaagtagt gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga
    21181 gatcacaaaa ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa
    21241 aaccaagatc aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt
    21301 cattttattt gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa
    21361 gtttttagat atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata
    21421 ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac
    21481 tgatttgctt tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt
    21541 gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca
    21601 aatcacccat caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga
    21661 aacacatacg caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa
    21721 gaaagacctt tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt
    21781 tattaatcag aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact
    21841 aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct
    21901 tttgttagat gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac
    21961 aatcgaagga aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa
    22021 aatgactgtt gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac
    22081 tgaatgacag aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt
    22141 caagtacttg aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact
    22201 agttctgagg gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta
    22261 ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt
    22321 attgacgacg gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt
    22381 gaaacggtct ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt
    22441 tctggtggac ttcacggagt aggatcttct gttgtaaacg cgctttctac acagcttgat
    22501 gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg
    22561 gacgacttaa agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca
    22621 gaccctgaaa tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc
    22681 cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa
    22741 gaaccagtca tcaatgaata ccattatgac ggcgggatca aaagttatgt cgaatacttg
    22801 aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat
    22861 atcgcagttg aagtctctat gcaatataca gacggctacc attccaatat catgagtttt
    22921 gccaacaata tccatacgta tgaaggtgga acacatgaat caggattcaa gactgcttta
    22981 actcgtgtga taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac
    23041 ttgaccggtg aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac
    23101 cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact
    23161 gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg
    23221 caaattgttg aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt
    23281 gaagtaacga gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac
    23341 tgctctagca acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc
    23401 ggatcagcta agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa
    23461 atcttgaatg ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct
    23521 ttatttactg ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac
    23581 cacaaattag ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg
    23641 ctgactcttt tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct
    23701 cagccacctt tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa
    23761 aatgcagaag aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc
    23821 gttcagcgtt ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg
    23881 gacccaagca atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag
    23941 atatttgaaa tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat
    24001 gcacactatg tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga
    24061 aatcagagaa aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga
    24121 ttatgcgatg agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc
    24181 cgttcatcgc cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca
    24241 taaaaaatct gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacat
    24301 tgcgatctat gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt
    24361 ggatggtcat ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac
    24421 cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt
    24481 tgatttccaa agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt
    24541 cccgaatctt ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc
    24601 acctcataac ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt
    24661 gacgaccaat gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt
    24721 tatggggaaa tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg
    24781 tgcaaaagtt gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt
    24841 gccttatatg gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa
    24901 gcgtatcgaa gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt
    24961 cattgacatc cgtcgtgacg ccagcgcatc cgttatcttg aataacttat ataaattgac
    25021 atccttgcaa aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt
    25081 tttgagcttg aaacaaatcc ttgaaaacta tatcgaacat caacgcgaag tcatcacacg
    25141 acggacggtc tttgaaaaag aaaaagcaga agcaagagca cacatcttag aaggtttgcg
    25201 tattgctttg gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga
    25261 cgaggcaaaa gcaacaatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat
    25321 tttagatatg cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata
    25381 ccaagaactc ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt
    25441 tacagaaatc atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg
    25501 cacagaatta ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga
    25561 agaaatcgtt attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt
    25621 cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt
    25681 cgtcaaaaat ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg
    25741 aaaagtgtat cgggcaaaag gatacgaaat tccagaatac gggcgtacag ccaaaggaat
    25801 cccaatcatc aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt
    25861 gacgggagaa gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa
    25921 acgtacttct gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt
    25981 gaaagaagac gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat
    26041 tggaacacat aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg
    26101 tacagcttcc ggggtcagag ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc
    26161 cttaatgaag gaaaatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac
    26221 aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat
    26281 cactgaaaaa aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat
    26341 gctgattaca gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg
    26401 acgttcaaca ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat
    26461 ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata
    26521 accatcaaga ctaagtgtgt aagctagcaa gaataaccga ttaaaaaatc tggttattcc
    26581 tgctggcttt tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat
    26641 tgcttttgac caaaatagag tgtataatta gagcttgtga gtagtctaat taattagata
    26701 actctccttg ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg
    26761 aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa
    26821 ctgctttaat cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat
    26881 caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct
    26941 atcacatcgt taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc
    27001 ttgctaaaat caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt
    27061 tgtttcatgt gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt
    27121 gtattagttg gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca
    27181 gtcgcaacat ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag
    27241 gctgatttta tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct
    27301 cgtaaaggaa cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag
    27361 caaggacaac gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt
    27421 cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct
    27481 ggcggatttg gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct
    27541 gatttcgacc gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac
    27601 gatgatttac cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag
    27661 aggcggacgt aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga
    27721 ttataaagat atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg
    27781 tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc
    27841 tcgtatcatg ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg
    27901 actgatcctt ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt
    27961 ttagtcgctc gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa
    28021 ttatggaaaa taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat
    28081 taatcgaagt attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc
    28141 tattgatgaa tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa
    28201 ataaacgtga aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct
    28261 cagaagtcat gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc
    28321 tcaatccatt agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag
    28381 atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat
    28441 ttcagtttga tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc
    28501 agttgttaaa actccatcag acgaacagtc agccggtgat tggtatttta tctattgata
    28561 actatgcaga tgccattgat aaattagatg acaaagagat ttcttatttg aactctttcg
    28621 tcactacact agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg
    28681 cagaacggtt tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat
    28741 ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta
    28801 gtatggggat tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata
    28861 atttagatgt tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag
    28921 gagcaaaacc tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc
    28981 gttctcgagc gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta
    29041 tcatggggca caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc
    29101 tggcacgctt caaccagaaa aaagcttaca tcgttatcaa tgagaacgag atcattccag
    29161 atgtagaacg gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct
    29221 ctccagaacg tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc
    29281 ataaaccttc tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca
    29341 ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatattgaat
    29401 cttcagcttc ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa
    29461 caaatcgctt agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa
    29521 atagtttcaa tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt
    29581 gtggagcaga cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg
    29641 agatcagcaa tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag
    29701 ggacagacga aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt
    29761 cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga
    29821 tcagtgccag aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg
    29881 gaggacactt cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac
    29941 agaagctatt tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca
    30001 aatgaaagta atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga
    30061 agttccagca ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac
    30121 gaaaagcagt atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga
    30181 aatcttggaa gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga
    30241 attgaaggcg aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat
    30301 tgctgaaggg ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga
    30361 aaatccgatc cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac
    30421 ggctacgatc aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg
    30481 gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc
    30541 aatgaaagca aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag
    30601 caaaatagca taaagaaaac gagtataata aaagagaact atttttttat tacactttcg
    30661 gtacgaatga tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc
    30721 gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca
    30781 agcggtttta ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat
    30841 agagccaaaa gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt
    30901 aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa
    30961 tttgttagaa gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac
    31021 agcagcaaat atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt
    31081 gatccagaca gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac
    31141 aattttagat gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg
    31201 ctttttatct attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca
    31261 aaacaatgaa gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc
    31321 aggtttccag cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc
    31381 gtttgcattg aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt
    31441 tagtttggaa atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat
    31501 tgaggccagc catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt
    31561 tgcgatgggg agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat
    31621 ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat
    31681 attgatcgat tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt
    31741 atcagatatc tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc
    31801 tctttcacag ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga
    31861 tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga
    31921 tgattattat gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa
    31981 cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact
    32041 gtttatcaaa gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag
    32101 aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca
    32161 ggaacgacta gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt
    32221 cacagtccct ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc
    32281 aaacgtcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
    32341 ctgatcttac gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
    32401 cacaatatca tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta
    32461 aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata
    32521 cgcccaatca aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
    32581 gcaggacagc tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa
    32641 ataagtcgaa atttcacaaa aatttgag
//
LOCUS       DADVXO010000016        31449 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.016, whole genome shotgun sequence.
ACCESSION   DADVXO010000016 DADVXO010000000
VERSION     DADVXO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.016"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            212..604
                     /locus_tag="KB950_000654"
     CDS             212..604
                     /locus_tag="KB950_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6856893.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="KB950_000655"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="KB950_000655"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBB6856894.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="KB950_000656"
     CDS             3165..4775
                     /locus_tag="KB950_000656"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBB6856895.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="KB950_000657"
     CDS             5067..5936
                     /locus_tag="KB950_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBB6856896.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="KB950_000658"
     CDS             6101..7372
                     /locus_tag="KB950_000658"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBB6856897.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="KB950_000659"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="KB950_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBB6856898.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="KB950_000660"
     CDS             8763..9026
                     /locus_tag="KB950_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBB6856899.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="KB950_000661"
     CDS             9471..9920
                     /locus_tag="KB950_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6856900.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="KB950_000662"
     CDS             9977..11395
                     /locus_tag="KB950_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6856901.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="KB950_000663"
     CDS             11395..12255
                     /locus_tag="KB950_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBB6856902.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="KB950_000664"
     CDS             12429..13202
                     /locus_tag="KB950_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6856903.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="KB950_000665"
     CDS             13349..13714
                     /locus_tag="KB950_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBB6856904.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="KB950_000666"
     CDS             complement(13862..15070)
                     /locus_tag="KB950_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6856905.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="KB950_000667"
     CDS             complement(15089..15367)
                     /locus_tag="KB950_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856906.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="KB950_000668"
     CDS             15521..16216
                     /locus_tag="KB950_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6856907.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="KB950_000669"
     CDS             complement(16658..17455)
                     /locus_tag="KB950_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBB6856908.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="KB950_000670"
     CDS             complement(17452..18636)
                     /locus_tag="KB950_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBB6856909.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="KB950_000671"
     CDS             complement(18661..19908)
                     /locus_tag="KB950_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBB6856910.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="KB950_000672"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="KB950_000672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBB6856911.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="KB950_000673"
     CDS             21888..22202
                     /locus_tag="KB950_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBB6856912.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="KB950_000674"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="KB950_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBB6856913.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="KB950_000675"
     CDS             22887..23531
                     /locus_tag="KB950_000675"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBB6856914.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="KB950_000676"
     CDS             23546..23875
                     /locus_tag="KB950_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBB6856915.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="KB950_000677"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="KB950_000677"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBB6856916.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="KB950_000678"
     CDS             24863..25687
                     /locus_tag="KB950_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBB6856917.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="KB950_000679"
     CDS             25680..26027
                     /locus_tag="KB950_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBB6856918.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="KB950_000680"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="KB950_000680"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBB6856919.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="KB950_000681"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="KB950_000681"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBB6856920.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="KB950_000682"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="KB950_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBB6856921.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="KB950_000683"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="KB950_000683"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBB6856922.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10111 a         5634 c         6632 g         9072 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatg
//
LOCUS       DADVXO010000017        31002 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.017, whole genome shotgun sequence.
ACCESSION   DADVXO010000017 DADVXO010000000
VERSION     DADVXO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.017"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            227..541
                     /locus_tag="KB950_000684"
     CDS             227..541
                     /locus_tag="KB950_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856923.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(594..1367)
                     /locus_tag="KB950_000685"
     CDS             complement(594..1367)
                     /locus_tag="KB950_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856924.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1472..1717
                     /locus_tag="KB950_000686"
     CDS             1472..1717
                     /locus_tag="KB950_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6856925.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1873..2724
                     /locus_tag="KB950_000687"
     CDS             1873..2724
                     /locus_tag="KB950_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBB6856926.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2757..3431
                     /locus_tag="KB950_000688"
                     /pseudo
     CDS             2757..3431
                     /locus_tag="KB950_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            3688..3876
                     /locus_tag="KB950_000689"
                     /pseudo
     CDS             3688..3876
                     /locus_tag="KB950_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3922..4887
                     /locus_tag="KB950_000690"
     CDS             3922..4887
                     /locus_tag="KB950_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6856927.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4909..5121
                     /locus_tag="KB950_000691"
     CDS             4909..5121
                     /locus_tag="KB950_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6856928.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5196..5672
                     /locus_tag="KB950_000692"
     CDS             5196..5672
                     /locus_tag="KB950_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBB6856929.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5669..7477
                     /locus_tag="KB950_000693"
     CDS             5669..7477
                     /locus_tag="KB950_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBB6856930.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7554..7769
                     /locus_tag="KB950_000694"
     CDS             7554..7769
                     /locus_tag="KB950_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBB6856931.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7861..8724
                     /locus_tag="KB950_000695"
     CDS             7861..8724
                     /locus_tag="KB950_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856932.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8761..9297
                     /locus_tag="KB950_000696"
     CDS             8761..9297
                     /locus_tag="KB950_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBB6856933.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            9311..9733
                     /locus_tag="KB950_000697"
     CDS             9311..9733
                     /locus_tag="KB950_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6856934.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9663..12062
                     /locus_tag="KB950_000698"
     CDS             9663..12062
                     /locus_tag="KB950_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6856935.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12094..14085
                     /locus_tag="KB950_000699"
     CDS             12094..14085
                     /locus_tag="KB950_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6856936.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14108..14359
                     /locus_tag="KB950_000700"
     CDS             14108..14359
                     /locus_tag="KB950_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBB6856937.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            14349..15578
                     /locus_tag="KB950_000701"
     CDS             14349..15578
                     /locus_tag="KB950_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBB6856938.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15575..17656
                     /locus_tag="KB950_000702"
     CDS             15575..17656
                     /locus_tag="KB950_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBB6856939.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17805..21725
                     /locus_tag="KB950_000703"
     CDS             17805..21725
                     /locus_tag="KB950_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBB6856940.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21726..22670
                     /locus_tag="KB950_000704"
     CDS             21726..22670
                     /locus_tag="KB950_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856941.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22674..22877
                     /locus_tag="KB950_000705"
     CDS             22674..22877
                     /locus_tag="KB950_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBB6856942.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23177..23413)
                     /locus_tag="KB950_000706"
     CDS             complement(23177..23413)
                     /locus_tag="KB950_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6856943.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23596..23982)
                     /locus_tag="KB950_000707"
     CDS             complement(23596..23982)
                     /locus_tag="KB950_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBB6856944.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(23964..25292)
                     /locus_tag="KB950_000708"
     CDS             complement(23964..25292)
                     /locus_tag="KB950_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6856945.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(25253..25582)
                     /gene="mobC"
                     /locus_tag="KB950_000709"
     CDS             complement(25253..25582)
                     /gene="mobC"
                     /locus_tag="KB950_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBB6856946.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25840..26210)
                     /locus_tag="KB950_000710"
                     /pseudo
     CDS             complement(25840..26210)
                     /locus_tag="KB950_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            26355..26540
                     /locus_tag="KB950_000711"
     CDS             26355..26540
                     /locus_tag="KB950_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6856947.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26698..26865
                     /locus_tag="KB950_000712"
     CDS             26698..26865
                     /locus_tag="KB950_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856948.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            27483..28142
                     /gene="vanR"
                     /locus_tag="KB950_000713"
     CDS             27483..28142
                     /gene="vanR"
                     /locus_tag="KB950_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBB6856949.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            28145..29506
                     /gene="vanS"
                     /locus_tag="KB950_000714"
     CDS             28145..29506
                     /gene="vanS"
                     /locus_tag="KB950_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBB6856950.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(29597..30898)
                     /locus_tag="KB950_000715"
     CDS             complement(29597..30898)
                     /locus_tag="KB950_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6856951.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         8135 a         7685 c         8044 g         7138 t
ORIGIN      
        1 gaagaaaaaa cgataaaagt tgtgataaac gatttaaaaa ataggagaaa atatatcaag
       61 cgcatggtat gaaacgcaca tttaaaaaga ttcgactaac taaaaaaata agtttacaaa
      121 gcattgttgt gtattacaag tgatctatac actgaaaaat gatagagaaa atctgatacg
      181 tccgcaacaa tggacaattg aaaatgaaag tgaggaaatt ggaagaatgg ctgatgaaaa
      241 taaagttgtt ccgcttgaaa cactagtgat aaataaggaa gagtttgagc aactttttct
      301 tgattttttt gttcaatatg acaggattta tgattttagt tcgtcactag atttatttga
      361 attagaggca gaaataatag aaaacagtcg agaggagaat ggtaaaatca tcatttcagt
      421 agaaaaattc aatgaattat tctcagaaac atttgaaata gatacagatt attatcaacc
      481 acaagaagtt ttccaatcat ttagaaatca gattattcgc tcaggtaaga ccgtaaactg
      541 aaaaacaata aaagaaacgt tgaaagaata attattcctt caacgtttct tttttagaat
      601 gttttaatga ccaaagcagt ttcattgtca ggcttaggta attcatgaaa tcccgtattt
      661 atagtgctca attgatcaac taaccatgtt ttactttttg aattgtagat aaatctaaaa
      721 ttaatatcgt tattatcgtg ttgaaggggc gtctccaaat aatctgctgt atattcatag
      781 tcaacgtaac ctgctaatac aaaagagagt atccctttat ttaatgttag atttaatgga
      841 taatcatacg actttatttt tttcaacttc atattgtaca tatcttcagc cataaaagtt
      901 gagttcgtga tttcatcttg ggtctcatta aaaaagttat ccgaataatc gcttattttt
      961 gaaaagtctt tattggcacc agcctcaaaa aattgttcaa ctttttcagt ataaagttgt
     1021 gctaacgctc ctttttcaat attaacttta atattttcat gtctatcaat ttgtgtgctg
     1081 ttcagtaagg gaagatcagc ttttagtttt atatcgtctt gatttctttc aatttcaatt
     1141 ggcaaaatct tttcagtaga ttcagtttct gataaaggaa catctagttt tttatcatga
     1201 taataagctt caaatccatc tggaaccgga aaaaaatgta ttgggtaatt cttttctgag
     1261 gttgttgtac tagtttccgt acttgtagaa cttgtgtcag aagtggtaca cccgcttata
     1321 gttccgatga gtaaagcgca agttagaatg attgcaaatt tcttcaaaaa aattcctcct
     1381 taaaattgaa tatggactaa tactagtccc attgtagcat accgaatctt taagattaat
     1441 aagaaaaagg actaatttta gtccaaaagg tgtgctattc ttgaaagagg aaaaaattat
     1501 tgataaagaa aatgtaatcg gtagcaatat tagacgagtc cgattagaaa aaggaattgg
     1561 tcaaaccgag ctgattagag acctacagct tcgtaaaatt gctataacta gggaaacgtt
     1621 ggttaagatt gaaggtggcc gccaacacat taagttggat caattgaaag ccatcaaaga
     1681 tattctacag gtgtcttatg aagatttgct tcaatgacta cttgtagtca tttcgtttga
     1741 aattattatg ttacaccctt atgtgtcacg aagaaatact ggtataataa tgaatgggat
     1801 atgtaacgat agggtatact gttatgttgc ctaataaatt gtggatcaaa atacaagaat
     1861 ggagggatat ccttgtataa ggcatatgaa ttggatttta caaatgctga cttaaatgca
     1921 ttctcctcaa gctcgcaatc atatacacat gtagtaaatc aaatagatca caaccaaaaa
     1981 tctattaaca agagaataga aaatctattt gaaagtgaaa atgatttagt tgattattat
     2041 tctaaggaca gcaaaattct agatgcagac gaaattatta aagattggtt tccaactata
     2101 aaagcagata tttttatatc tcactctcat gctgatgaaa aattagctat acgttttgca
     2161 tcatggctat ttgaaaattt tggtcttact gcatttattg attcttcggt ttggggatac
     2221 agttctgatt tattaaaaaa aatagaccaa aaatattgct ataaggaaca aacaaaaaca
     2281 tatgattatg ataaaagaaa tgtaacaact agtcatgttc atatgatgct ttctacagct
     2341 ttaaataata tgattgatag tacagaatgt ctgttttttc ttaatacacc taattcaatt
     2401 agtttgagca atgaaatcac taatgaacag aaatttacgt attcgccatg gctatatagc
     2461 gaactgacga cagcttcaat agtggaaaaa aagaatccac gattggaaag taatcctcaa
     2521 atgagcaccg aggatgttag aagcattata aagcactatt cttcgcaaga aaatctgcaa
     2581 ataaaatatt ctttaaattt aagtgaatta acttatttac gaaaagctga tttgaaaaaa
     2641 tgggctttag atgctgtgga atgcactaaa ggaagtaata cattagatat tttatataaa
     2701 aacaccattt tcactaatct ctaatcaatt tactgaatcg aaaggaaccg ggtagaatgg
     2761 ataagaaagt tttaataaag aggttctctg aagctattga aaaaggaaat gcagcaatat
     2821 ttgcaggtgc aggattatcg atgtcacaag gatatgtaag ttggccggaa ctacttaatg
     2881 atcctgcaac agaaatcggg ttggattcta aaaaagaaac agacttagtt actctggctc
     2941 aatattataa aaatgaaaat ggtggatcaa gaggaatttt gaatcagata cttatggata
     3001 attttggtga agaattagaa atatctgaaa atcatcgaat actagcaagt ttacctattg
     3061 aaacttattg gacaactaat tatgatcatc taatagaaaa atcaatccga gaagcttata
     3121 aaaaccctca ggttaagaaa aattatactc agttagcaac aactaatcca aatgtggata
     3181 caattgttta taaaatgcat ggtgatatag atgatgtcag ttcgacagta ataacaagag
     3241 atgattatga aaaatatgat gattcgtacg cgttatttaa agaaacttta aaaggagatt
     3301 tgttaacaaa aacttttctc tttttaggat ttagttttac agaccctaat ttagaaagaa
     3361 ttttatcaga tataagatgg gttttgagag aaaaccaaag acctcattat tgtataatga
     3421 gaaaaatatt atagaaaaaa ttttaggtta tcgctgaaag ccccggaaat acgggcattt
     3481 cgcaggatat gagaactcaa tttactacca atttactact ttcggattga gccgcatata
     3541 tgctgaacca tactgctaat acccgccacc ccaaatacgg cacatcataa attgaatacg
     3601 acaccgtaaa cgcggcgttc ctctttcccg tcatgggaga acaggacgcc gcttttttat
     3661 gcccaaatag agaaaggagg cgacgccatg ctatttactt ccggccctga ccgggcgttt
     3721 gaacggctga tgcaggaaaa gcccggtttc gatcactacg acggcggcgg ggcggacgcg
     3781 ccggaggatt gcggcacctg ccgattttac cgcccggatt ggaaatatca attctgccag
     3841 tttgcagagt gtccctatca gccgggcaag ctgaccgact atgcagcggt caaattcaca
     3901 gtgaaaggag cgaacaacgc tatggcagtt ttccgtgtgg aaaagaacaa aggctatacg
     3961 gtgatgtcaa atcaccacct gcggaataag aacctgacct taaaggccaa gggcctgctg
     4021 tcgcagatgt tgtccttgcc ggaggattgg gactataccc taaaaggact gtcctatatc
     4081 aaccgggaaa gcatcgacgc gatcaggaca gcggtatggg agcttgaaaa agctggatat
     4141 atccggcgtg agcagggccg tgacgcaaaa ggcaaaatgg ccgatatggt ctataccatc
     4201 tacgaacagc cggtattgga aaacccggta ttggaaaatc caacatcgga taacccggtg
     4261 ttggaaaatc cgacatcgga taatccggcg tcggaaaatc caacgcaatt aaataaagaa
     4321 gtacaaagaa ctgacttatc aaaaaaagaa aaaataatta cagatggaca aagtacccat
     4381 tccattccta tcccatcccc gacccctgcc cctatggagg gcgaggcagc ggctacgccg
     4441 ccggaacgga aaaggacagg aaaggatgag gctgtacgga tttatcggga aatcattttg
     4501 gagaacatcg agtatgccta tctcattcag gacaggagca ttgaccgtga acagcttgac
     4561 gaaatcgtag acctgatgct ggaaaccgtc tgcaccgccc gaaagacaat ccgtgtcgcc
     4621 ggggacgact accctgccga gctggtgaaa tccaagtaca tgaagctgac cggcgaacat
     4681 atccgctttg tccttgactg tctgcgggag aacacaacga aagtccgcaa tatcaaacaa
     4741 tacctgcggg ctatgctgtt caacgccccc agcaccattt ccaactacta cacggccctt
     4801 gtcgcgcacg acatggcaca gcccgattgg ggaaagccta aatccggcct gccggactat
     4861 tcctgcggcg agggcgaaag cctgtaacga gaaaaggagg acgaccctat gaagcagggc
     4921 gcattgattt ttgacgagcg aacagaccgt tacgacatcc gctttgatct ggccgactat
     4981 tacggcggcc tgcattgcgg ggagtgtttc gacgtgatga tcggcggcaa atggaggccc
     5041 acccgcattg aaatggccga aaactggtat cttgtcggca tccgggccga cgacctttcc
     5101 ggcctgcggg tgcggatttg acctatatct acctgcggcg tctggtggtt ccgccttaga
     5161 cgccgctttt gactttctga caaggaggga tgcgattgca ggaagaagtt actcaaaaga
     5221 ccattgccct gtccatgaaa acgggcaagc tcacggctca agtgctgcaa gcggcgctga
     5281 aaaagtacct gcaacaccgg gccaagggca aaaacacgct gcaccacggc cagcagagcc
     5341 taaagcagct taaaaagcat ggggctgatc tctccaacat tgagatcacc gaggccaaca
     5401 ttggagcgtt caagccatgc gccaagaaat acggcgtgga ttttacctta cgaaaagata
     5461 agaccaccca gccgccccac tacgttgtga ttttcaaggc caaggatgca gataaccttg
     5521 atctggcgtt tcgggagttt accgccaaga cgctttccaa ggagcagcgg ccctctatcc
     5581 gcaaggtttt gtccgctgtg aagcaaaagg cggcggccca gaccaagcag cgggccaagg
     5641 agaaaatcaa ggaaaggggg ctggaactgt gaagcctgac gtaaagaaat tgctgatcct
     5701 gaacctcccg tatctgattt ttgtgtacct gtttgcgaaa tgcggggagg cgtaccggct
     5761 ggccgctggt gctgacctgt cggggaagct cctgcatttt gcggatggtt ttgcggcggc
     5821 ttttgccaac ccgctgccaa gcctgcacct gtttgacctg tgtatcggta tcgccggtgc
     5881 cgtgatcgtg cggctggtgg tgtactgcaa gagcaagaac gtgaagaaat accgcaaagg
     5941 catggagtat ggcagcgccc gctgggggac ggccaaagac atagcgcctt acatcgaccc
     6001 caagtttgaa aataatatcc tgctcaccca aacggaacgg cttaccatga ccggcaggcc
     6061 caaagacccc aagacagcgc ggaataaaaa cgtgctggtg atcggtggca gcggttcagg
     6121 gaaaacgaga tttttcgtga aacccaacct gatgcaatgc tttccgacca cggactatcc
     6181 gacttcgttt gtggtgacag atccgaaagg cactttggtt ctggaaaccg gcaggatgtt
     6241 ccagcaggcg ggctaccgca ttaaaatcct gaataccatt aacttttcca agtccatgaa
     6301 gtacaacccc tttgtctaca tccattcgga aaaggacgtg ctaaagctgg taaataccct
     6361 gatttccaac accaagggcg agggggaaaa gtcagcggag gatttttggg tgaagtcgga
     6421 acggttgttt tattcggccc tgatcggcta tatctggtac gaggccccag cggaggaaat
     6481 gaacttctca acgctgctgg aaatgataaa cgccagtgaa gcccgcgagg acgacccgga
     6541 gtttcaaagc cccgtcgatc tcatgtttga gcgtttggag cagaaagacc ccgaccattt
     6601 tgcggtgcgc cagtacaaga aattcctgct gtccgcaggc aagacccgtt cttccatcct
     6661 gatttcatgc ggtgccagac ttgcgccctt tgacatccgg gaggtacgcg agctgatgga
     6721 ggacgacgaa atggagctgg acaccatcgg ggacgaaaag acggtgctgt atctcattat
     6781 gagcgatacg gacacgacct ttaatttcat tctggctatg ctccaaagcc agctaatcaa
     6841 cctgctttgt gaccgggctg atgatgtgta cggcgggcgg ctgcctgtcc atgtacgcct
     6901 gattttggat gaatttgcca acattggcca aatacccaat ttcgataaac tcattgccac
     6961 cattcgaagc cgtgaaatat cggcctctat tattttgcag agccagtcgc agctaaaagc
     7021 gatttacaag gacgccgccg agatcatttc ggataactgc gatagtgtgc ttttccttag
     7081 tgggcgcggc aaaaatgcca aggagatttc cgatgcgctg gggaaagaaa cgattgacag
     7141 tttcaacacc agcgagaacc ggggaagcca aacgtcccac ggattgaact atcaaaaatt
     7201 aggaaaggag ttgatgtccc aagacgaaat tgccgtgatg gacggcggca agtgtatctt
     7261 gcagctacgg ggtgtgcgcc cgtttttatc ggagaaattc gatattacca agcatccccg
     7321 gtacaaatac cttgccgacg ccgacaagaa gaataccttt gacgtggaca gatttttgac
     7381 tatcacgcgc cggaaacggc agcaagtggt aacgcaggat gaagtattcg acctgtatga
     7441 aatcgacctg tcggacgagg acgccgccgc tgaataaaca gcggcttttt tcattccaac
     7501 aacaaaatca aaaaatcatt atgagccgga aaccggcaga aagaggtaaa tttatggcat
     7561 tttttaattc cgcagttact actttgcaga ctatcgtgat cggactgggc ggcgctctgt
     7621 gcgtatgggg cggcatcaat ctgttggagg gatacgggca ggacaatccg ggggcaaaat
     7681 cgcagggtgt caagcagctt gtcgctggcg gcggcgtggc cctgatcggt gtcactctgg
     7741 ttcccctgct gtccgggctg ctgggctgat cctggccttt gtccctgtcc aaacccttaa
     7801 ctaatcgcct ccgcgtcctc ccggccagac cgggagggcg cgggaaagga ggtgctaccc
     7861 gtgattagtg aaatcattga agaatggata aaaggcatcc tcatggacgg tatcacgggc
     7921 aacctgtccg ggctgttcga tacggtaaat gccaaagtcg gagaaatcgc cgccgacgtg
     7981 ggacgaaccc cgcaggcatg gaacagcggt attttcaata tgctgcacag cctgtcggaa
     8041 accgtgatcg tccccatagc cggggccata ctcgcccttg tcatgtgcta tgagcttatc
     8101 cagatgataa cggaaaagaa caatatgcac gactttgaca gctccatgtt cttccgctgg
     8161 atattcaaat ccgcgtttgc aatcctgatc gtaacgaaca catggaacat cgtcatgggc
     8221 gtattcgacg ccacccaaag cgtcgtcaat caaagcgccg gtgtgattat cgggcagaca
     8281 tccattgatt ttaacaccct gataccagac cttgaaagcc aattggaggc tatgagcgtg
     8341 ggcgctcttt tggggctgtg gttccaaacc cttgttgtgg gcctcaccat gaatatcctt
     8401 tccatctgca ttttccttgt gacctatggc cgcatggtgg aaatctatgt tgtcacgtcg
     8461 ctggggccta tcccccttgc cacaatgggc aacgcggaat ggcgcggcat gggccaaggg
     8521 tatctgaaat ccctgctggc gctgggcttc caagcctttt taatcatggt tgtggtgggt
     8581 atctacgcgg tgctgataca gaatatcggt tccgcttccg acatatccgg cgcaatctgg
     8641 ggctgtatgg gctatacggt gctgctgtgc ttctgcctgt tcaagaccgg cagtatatca
     8701 aaggccgtat tcggggcgca ttgatcccaa aatccaagca tacacaggag gaacttgttt
     8761 atttcaacgt acaacatcat ttacgctgac ccgccgtggc ggtatgactg caaaaacgta
     8821 cagggcgcag cggaaaatca ctattccacc atgagcattg acgagctgtg cgccctgccg
     8881 gtggaacggc tggcgtccaa agactgcctg ctgtttttgt gggccacatt ccccatgctg
     8941 ccggaagctc tgcggttaat caaagcgtgg ggcttttcat tcaagaccgt ggcttttgta
     9001 tggctgaaac agaaccgtaa aagccttaca tggttttacg ggctgggccg ctggacaagg
     9061 ggaaacgcgg aaatctgcct gctggcaaca cacggcaagc cgaagcgtcg ttctgcctct
     9121 gtccatcagt ttattatcag cccgattgaa caacacagca aaaagccgga tgtgacccgt
     9181 gaaaaaatcg tagagctggc gggcgatttg ccccgcgtgg agctgtttgc cagacagaaa
     9241 acccccggct gggacgtgtg ggggaacgag gtggaatgtg accttatcct gacgtgaaag
     9301 gaggtgccaa atggcttatg taaccgtccc caaggactta acccatgtga aaagcaaggt
     9361 tgtgtttggg cttaccaaac ggcaactgat ctgtttttcc ggggcgctgg tgatcggcgt
     9421 ccccttatat tttctcaccc gtgactatct taccaacagc gcggcggccc tgctcatggt
     9481 atttgccatg ctgccgggct ttttgcttgc catgtacgag cgtcacggcc agccccttga
     9541 agtggtgatt atgcagatca tccagtgctg cttcatccag ccaaaggagc gtccctatca
     9601 gaccgacaac acctacaccg cccttgtgcg gcaataccag atggaacagg aggtaaaggc
     9661 cattgtccaa aaaggaaatc ccaagagaaa ccgaaaaaac aaagctgacc cgcgcccagc
     9721 gaaaagaaat tgacgcggta atccgtaaat ataagggcga cggcaggccc cacacggcgc
     9781 agcagagcat cccctacgag gtcatgtacc cggacggcgt gtgccgggta tcgcccggcg
     9841 tcttttccaa gtgcattgag tttgcggaca tcagctatca gcttgcccag ccggacacgc
     9901 agacggctat ctttgaaaaa ctgtgcgacc tgtataacta tgtggacgcc tccattcata
     9961 tccagttttc tttcctcaat cggaaagttg acccggtgca gtacgccaag agctttgaga
    10021 tcgcgccgca gggggacgac ttcgacgaca tccgggccga gtacaccggc attctgcaaa
    10081 agcagcttgc aaacggcaac aacggcatgg taaagaccaa atacctgaca ttcaccatcg
    10141 aggccgaaag cgtgaaagcg gcgcgagcca gattgaagcg catcggcttt gacctgttag
    10201 gctatttcaa gagcatgggg gccgtagccc atgtgatgga cggctgggaa cggctgaacc
    10261 tgctgcatgg cgtgtaccac ccggacggcg agatattcaa ttttgactgg aagtggctgg
    10321 ccccgtccgg cctctccacc aaggatttta ttgccccgtc ctccctgtgc ttcggcaatg
    10381 caaagacatt cggcatgggc ggcaaatacg gcgcggtaag tttcctgcaa atcctgtccc
    10441 cggagctgtc ggacgatatg ctggccgact tcctcaatac ggaaagcggt gttttagtca
    10501 atctccatgt gcaggccatt gaacagacca aggcgatcaa gaccatcaag cgcaaaatta
    10561 ccgaccttga cgctatgaag attgcggagc agaaaaaggc cgtccgcagc ggttacgata
    10621 tggacattct tcccagcgac cttgccacct acggcgagga cgccaagaag ctgctgacaa
    10681 agttgcagac ccgcaatgaa cggctgttcc agttgacgtt ccttgtcctc aacgtggccg
    10741 acaccaagca aaagctgaat aacgacgtgt tccaagccgc aggcgtggcc caaaagcata
    10801 actgtcccct tgtccggctg gactaccagc aggaacaggg ccttgcaagc agcctgccgc
    10861 tgggcgtcaa tcagatcaag atacagcgca gccttacgac ttccagcgtg gccgtgttcg
    10921 tcccctttgt gacgcaggag ctttttcagg gcggcgcggc catgtattac ggcatcaacg
    10981 ccaagtcccg caacatgatt atgctggaca ggaagcaggc ccgttgcccc aatgccttga
    11041 agctcggcac ccccggcagc ggcaaatcca tgagctgtaa atccgagatc gtcagtgtgt
    11101 tcttaacgac gcctgacgat atttttattt cagacccgga ggccgaatat tacccccttg
    11161 tcaaacggct ccacggacag gtaatccggc tgtcgcctac cagcaaggac tttgtgaacc
    11221 cgctggacat caacctgaat tattcggagg acgataaccc gctggccctg aaatccgact
    11281 ttgtgctgtc gttctgtgag cttgtcatgg gcggcaagaa cgggctggaa gccattgaaa
    11341 agaccgtgat agaccgggct gtgcgcgtga tctaccggcc ctatctggca gacccccgcc
    11401 cggagaatat gccgatcctg tccgacctcc acaaagcgtt gttagatcag catgtcccgg
    11461 aggctgaccg ggtggcgcag gcccttgacc tgtatgtgtc cggctccctg aacgtattta
    11521 accaccgtac caacgtggac atcggcaacc ggcttgtgtc ctttgacatt aaggagctgg
    11581 gcaagcagct caaaaagctg gggatgctca ttgtccaaga ccagatatgg gggcgcgtca
    11641 cggccaaccg aagccaagga aaggccacat ggtattttgc cgacgagttc catttgcttt
    11701 tgaaagagga acagacggcg gcctattctg cggagatttg gaagcggttt aggaaatggg
    11761 gcggcattcc aacaggtgcc acccagaacg tcaaagacct gctttcctcc ccggaaattg
    11821 aaaatatcct tgaaaacagc gacttcatta cgctgctcaa tcaggcgtcc ggggatagaa
    11881 aaatccttgc ggaacgcttg aatttgtcca ccgaacagca gaagtacatc gacaattccg
    11941 aacccggcga gggccttttg atctttgaaa acgtggtgct gccctttacc aaccccatcc
    12001 cgcacaacac ccagctttac aagattatga cgacccgctt aaacgaggtg gcgggagtat
    12061 gacccggcga cgccacaaag gaggtgaacc accatgaaag agctgaaagc caaggcgaaa
    12121 gtgacgcagg ccatgacccg cgacgggctg acgctggaaa atcaggccac cggcgaggtg
    12181 gagaacattt ccagccggga ggccgaacag gatttgtccg cgccggggcc gggcggcacc
    12241 gccgataagc tgctggaacg ggccgacgcc atccatgacg gccataaggc caagaaagcc
    12301 gcgaagcagg ccggggctgt ggccgccgag ggggacgctg ccctgcaccg gccatccgcc
    12361 cggctccaac tgtcggagga agaacgggcc gacccggatt tacagcccta tatccgaaaa
    12421 tcggacaaac gggccgataa gctggataag gcccgcgccg ccatccccaa gaagcgcgtc
    12481 ccggtcaagg aaaccgtctt tgacgaggca agcggcaaag caaagaccgt cctgcacttt
    12541 gacaaacagg acaaggggcc tccctccctg aaacccaatc cggcaggccg tcctgtttcc
    12601 gaggtttttc tattcggcca cgggaaaatc catgaagtcg agcatgaaaa cgtgggtgtg
    12661 gagggcggcc ataaggggga ggaacttgcc gagcggcagg ccggaaaagc catccgcagc
    12721 accatccggc accacaggct gaaaccctac tgggacgcgg aaaaggccga gcgcaggcta
    12781 ttgtctgcca acgcggagta tttctaccag aaatctttgc gggataaccc ggagatcgca
    12841 caggcggcca gcaaccccat ttcccggttc tggcagaaac agcagatcaa gcgccagtac
    12901 gccaaggccg cccggacagc gggccagacc gcaggcgcgg cggcacaggg cgcggccaag
    12961 acagccgggg cctccacgaa agcggccaag gccgcagcca aagaaagtga acgggcggcc
    13021 tcctttgtgg cccgccactg gaaaggggcg ctggccgtcc ttgtgttggg gcttttgatg
    13081 ctgcttatca tcggcggctt acagtcctgc accgccatgt tcggcagcgc cggaagcggc
    13141 atcacggcgt cctcttactt ttccgaggat agcgatatgt tggccgccga ggacgcctac
    13201 gcggagctgg aagccgagct gcaagactat ctggacaact acgaggccac ccatgactat
    13261 gacgaatacc attttgacct tgacgagatc agccatgacc cctatgtgct gatttccatt
    13321 ctgtcggcgc tccatgacgg gatatttacc atcggggagg tacagggtga tttgtccatg
    13381 ctctttgaca agcaatatat cctgacagaa aacgtcgtgg tagaaacccg ttaccgcacc
    13441 gaaaccgata catggacgga cgcggagggc aacacccata cagacaccta ccaagtgccg
    13501 tatgactatt acatctgcta cgtcacgctg gaaaacttcg acctgtccca tgtgccagtc
    13561 tatatcatgg acgaggaaac gctaagtttc tattccctgt atatgtccac gctaggaaac
    13621 cggcctgatc tgttttccgg cagccagtac cccaacgcct ccaccctgac gccgccgacc
    13681 tactacgacg tacccccgga ggctttggag gatgccacct ttgccgccat gctcaaagag
    13741 gcggaaaaat atatcggtta tccctatgtg tggggcggca gctctcccag caccagcttt
    13801 gattgcagcg gcttcatttc gtgggtaatc aatcatagtg gatggaacgt gggacggctg
    13861 ggggcgcagg gcctatataa catctgcacc cctgtttcca gttcgcaggc caagcccggc
    13921 gaccttgtat ttttcaaggg aacctacgac acccccggcg tgtcccacgt cggcctgtat
    13981 gtgggcaaca atgtgatgct gcattgcggg aacccgattt cctacacgaa ccttacttca
    14041 aactactggc aatcccattt ttacagttac gggcgtttac cttaacgcca gaaaggagct
    14101 ttcaattatg gcaaccaaac ttgaccgcat cgaaaaggac atccagaaaa ccaagtctaa
    14161 gatcgcggag tttcagaaac agcttcggga gctggaaacg cagaaaaccg aacaggagaa
    14221 tttgcagatc atccagcttg tgcgcggcat gaacatgaag ccggaggaat ttgccgcgtt
    14281 cttacgcagc ggcgcaatgc aggccgcccc tgccgcaacc ccttaccatg aacaggagga
    14341 caatgcccat gaagaataag accagacgga tttcccgtat tctccccgca cttctggccg
    14401 tgatgctctg cgtcacggcc tttcccgtta ccgcccttgc aggcggcaat gacccggcac
    14461 cggccccgct gccggaggct acaaccgaac ccgctacggg cggtttggag cctgaaacag
    14521 acgaaaccgg caccgccacg gacgggacag aaaccgaggc cgaaaaagat accggccctc
    14581 tgaccggcaa ggacatcacc gacctgtttt ccgcgctgtt cggttccaag gtgagcattg
    14641 ccgccacgga cgacggcatc cagatcacca ccggcaccga caaaaaagaa cctgaacaga
    14701 ccggcaccgt caccaccaac ggcggcaggc tgaatgtccg caccggcgcg gggcttgaca
    14761 agaccgcgtt cacccagctt cccaatggga cgacggttga ggtagtcggc acagacggcg
    14821 actggattaa aattctcttg ccggagcgca tcggctatgt ccattccgac tatatgaccg
    14881 tcagcgaaaa ggaagtagcg gctaccggcg agggcggttt ttccctctcc attgacccgg
    14941 aggaaattgc ttctctgctg gaactgttca atggcggcgg ggccagcgcc gccctgtccc
    15001 cggacgggaa cctgtcactg attgacgaca tcggcagcgc cgcgaaaaat ggcaagcagt
    15061 ttatcaccgt ggaaaccaaa aacggcaatg tgttttatct gattattgac cgggacgacg
    15121 agggaaaaga aactgtccat ttcttaaatc aggtggatga agccgatctg ctgacgctga
    15181 tggaggacgg ggaaaccgtc acccccgctg ctgtctgctc ctgtaccacg aaatgcaagg
    15241 ccggggctgt gaacacgaat tgcccggtat gtaaaaccaa cctgaccgag tgcagcggcc
    15301 cggagccgca ggaaccccag ccggaggaac ccgaagcccc ggaggaagaa cccaagggcg
    15361 gcgcgggcgg cctgatcgtg ttcctgctgg tggcgctggc gggcggcggt gcggcccttt
    15421 actatttcaa gttcaaaaag cccaaggctg aaaccaccgg cagcgacgat ttgggcgagt
    15481 atgattttgg ggaggacgag gatttggagg acgaggaacc cgaaaccgag tttgacccgg
    15541 aagctgattt tgtgagtgaa caggaggacg aggaatgaca tataaacttg taatcgcgga
    15601 aaaacccagc gtggcccgca gcattgcggg cgtgatcggg gccacggaga aacacgacgg
    15661 ctatttggag gggaacggct atctggtgag ctggtgcatc ggacatctgg tgtcctttgc
    15721 cgacgccgga cgctatgacg agcgtttcaa aaaatggcgc tatgaggatt tgcccattct
    15781 cccggaaaca tggcagtaca tcgtccccga cgacaagaaa cagcagtttg acgttctctg
    15841 ttcgctgatg cagcgccccg acgtgaccgg ccttgtgtgc gccaccgacg ccgggcgcga
    15901 gggggaattg attttccgtt tcgtctacca gatggcggga tgccaaaagc cctttgagcg
    15961 cctttggatt tcctctatgg aggacagtgc tattaaggac ggctttgccc acctgaaacc
    16021 gggcggcgac tatgatccgc tgtatcaatc ggccctgtgc cgggccaagg ccgactggct
    16081 gatcggcatt aacgccaccc ggcttttttc cgtcctgtac cacaagaacc tgactgtggg
    16141 ccgcgtccag acgccgacct taaaaatgct ggtggacagg gacgccaaga ttaccgattt
    16201 caagaaagag aaatatcaca tcgtccatat cacggcgggc ggcgcggacg cggtaagcag
    16261 ccgcttttcc gacgctgccg aggcaaacac cgtcaaggcg gcttgtgcaa aagcgcaggc
    16321 cgtttgcgtt tccgtgacgc gggagaaaaa gacggaacag ccgcccaagc tctatgacct
    16381 taccaccttg cagcgggagg ctaaccggct tttcggctat accgccaagc agacccttga
    16441 ctatgcccag gcactgtatc taaagaagct gctgacctat ccgagaacgg atagtcaata
    16501 tcttaccgac gatatgctgc ccgccgtgga aagccttgtg tccggcctgt gggagctggt
    16561 gccgtttgcc aagggcctga acctttcccc gcagtttgac cgcatcctga acagcaaaaa
    16621 ggtgtccgac caccacgcca ttatccccac cgctgaattt gtgaagcagg gctttggcac
    16681 tctggccgag agtgaaaaaa agctgctgtc cctgatctgc tgcaagctct tatgtgccgt
    16741 ggccgggccg catatctatg aggccgtcac cgccacattt acctgtgccg ggcaggagtt
    16801 taccgccaag ggcaagaccg tcctttcccc cggctggaaa gagattgaac gccggttccg
    16861 ttcttcctta aagaccgacg ccgacgagga cgccgaggcg gtgcgggagc tgccggagct
    16921 ttgcgagggc cagacctttg cagacgtggc cgcctctgtc accgagcatt tcaccacgcc
    16981 gcccaagccc tacaccgaag atacgctttt gtccgctatg gaacgggccg gggccgagga
    17041 tatgccggag gatgccgagc gcaagggatt aggcacaccg gccacccgcg ccgccattct
    17101 ggaaaagctg gtgcagatgg gctttgtcca gagaaaaggc aagcagctca ttcccaccaa
    17161 ggacggcatc aatctggctg tggtgctgcc ggaggcgctt acttctcccc agcttactgc
    17221 cgaatgggaa aaccggctga ccgagatcgc caaggggcag gccgatcctg acgaatttat
    17281 ggacggcatc gagacgcagg cgcgggagct ggtgcagacc tattcctgta tcagtgagga
    17341 aaagcagaaa ttgtttcagg ccgagcgtgt tcctatcggg acgtgtcccc gctgtggtga
    17401 ggctgtctat gagggcaaga aaaactacta ctgcggaaac cgggcctgcc agttcgtcat
    17461 gtggaagaat gaccgatttt ttgaggaacg caagaaagcg ttcaccccga agatcgccgc
    17521 tgcgctgctc aaaaccggca aagtcaaggt aaagggcctc tattccgtaa agaccggcaa
    17581 aacctacgac gggacggtgc ttttggctga taccgggggc aagtacgtca actaccgcat
    17641 tgaacacagg agctaaagga cagtaacaca gcaagcatag ggagtgtggc tacactcccg
    17701 gtaaaatccc ccggccgcgt ccctgccggg ccggggattt ttgcttgttg gggaagtccc
    17761 caacccccta ttttttgaaa cagaacagag aaaggagtga ccgcgtgaac gaagtatttt
    17821 ctatccaagt ccataaccgg cagtttttgg aacagggcaa acaaggcgtc tggctggatt
    17881 tgcccaccac aaccgaaacg ctgcaagccg ccctgcggga tgttggcatt actgccgaca
    17941 atccgcagga tttttttatc aacgggtatt cctgcccgga ggacagacac cttgccattc
    18001 cctatgacat ggtgctggcc gccgacgtgg acgagctgaa tttccttgcc gcccggctgg
    18061 gccagcttga cgccgccgag atcgcagagc tgaacgccgc cctgcaaaat ccccgtggcg
    18121 gctttgagaa tatcggccag atcatcgact acaccgaaaa tgtggattac tatgtgcatc
    18181 ttcccgacgt taggggggat ggacagttgg gcgactacta cctgaaccgt tccggcatgg
    18241 tggatatgcc ggaggaatgg aaagccggga ttgatacggc gcagtttgga cgccacattg
    18301 cccagcagga acaaggcgtt tttacccagt acggctatct ggtgagaagc ggcgacgaat
    18361 ggcagcgcgt ccatgagggc cagccggtgc cggaggaata ccgggtgatg cactaccccc
    18421 agccggaagc tgaccgggag gaaccgcagg cccagcagcc ggaggcatcc gaaagccccg
    18481 acaaagcgcc gcccccggtt atcccgatca tgctggacgg catgaacagc aaagaacgca
    18541 tgaaagtgat tactgaccgt ctggaaaccg gcgtacagga gctttttgaa agtagccgct
    18601 atatggctta cttatccgct atgtccaaga tgcacgatta cagtctgaac aatacgctgc
    18661 tgatcgccat gcagggcggt tcgattgtgg cgggctataa caggtggcag gatgaatttg
    18721 accgccatgt gaagcggggc gagaaaggca ttaagatact ggccccggcc ccgttcaagg
    18781 ttaagcagca gatggagaaa accgacccgg ccacccagca gcccgtgatc ggcgcggacg
    18841 gcaagcccgt gaccgaagaa caggaagtga cggttccggc ctttaaggtg gtgacagtct
    18901 ttgacatcag ccagaccgag ggcaaggatt ttcccatgcc ctatatcagt gaacttaccg
    18961 gcgacgtgca gcagtatcag gatttctttg ccgcgctgga acggacatca ccctatccca
    19021 taagcattga gccgatttca gacggggcca agggccgctg cttctacgca aaacagcgca
    19081 ttgccatcaa cgagggcatg agcgaattgc agaccctaaa gaccgccatc catgagattg
    19141 cccatgccaa gctccacgcc attgaccggg aggcccccgc cgaggaacag gcggcccggc
    19201 cagaccgcag cacccgcgag gtacaggccg aaagcgtggc ctataccgtc tgccagtatt
    19261 acgggctgga cacgtcggac tattcttttg gctatgtggc cgattggagc agcggcaggg
    19321 agttatccga gctgaaagcc tcccttgaaa ccatccgggc cacggccagc gaattgataa
    19381 ccgagatcga cggccatttt gcccagcttc agcaggagcg cgaggcccgg caggaacagg
    19441 aggccgtcac ggttgacgtt accccggagg cagcggcccc ggcgcaggcc gaggaagaac
    19501 aggccccggc ccttgatccg gcagccgagc cgcttgtgac tgtgctatgg agcgaaagcc
    19561 cccagctaaa ggacggccag caaatgccgt tgcatgaggc cgacgcgctg tttgcccgtc
    19621 tggatgcagc atatccggca gaggcaggct atgacaaaac cgcgttccgc attgactgta
    19681 tctttcaggg cgagccggac agctacgagg ggcggcagga ttttggagat agggacggtt
    19741 ccctgatcga acatatccag agctaccacc aattttacgc gcaggacgaa aactggaaaa
    19801 agcatgtaat cgctacaacc ggcatagagg gctgggagca ggacaaggcc catcgggata
    19861 tgctgttagg cgagtttgtc ccctatatga agctgcattg cagccttgcc caaatggaac
    19921 agacggcgca gggccgctta cagtccgggg aaaccctgac gccggaggaa acggcctatt
    19981 tgggggcgct tctctcttat gtgcaggagt gccgccccat gctcaatcag ggccagtatc
    20041 agttgccgga gccgccgaaa ctggctgact tcgaccagag cttgcaggac tacaaagcac
    20101 aggtacaggc cgagatcgcg caggaggccg ccgcagccgg tatgaccgtg gaggaatacg
    20161 cggcggctgg ctatgaagca ccccaacagg acaccttttc catctaccag cttcgaggcg
    20221 gggaggaaac ccgcgactat cggtttgagc catacgagcg tttacaggcc gcagggctgg
    20281 ccgtggacag tgccaactat gaccttgttt atacggcacc gctggataaa agcacgtctt
    20341 tggaggatat ttaccgcaca ttcaacatcg accacccggc agattttagg ggacacagcc
    20401 tttccgtttc cgacgtggtg gtgctgcacc agaatggaca ggacaccgcc cactatgtag
    20461 acagcttcgg ctatatggaa gtgccggagt ttttgcggga aaatcccctg cgtaccgccg
    20521 aactgtctac ggagcagaac gaaaacatga ttgacggcat tatcaacaat acgccgcccg
    20581 aaccgcagcc ggaggaaccc gaacagctat cttttgagga tgtggcagtt gcttccggcc
    20641 agacacagga ggaacccccg gaggctgcgc ccccgcaaac ggctgtccgc tattatccca
    20701 tcaatgaggg agccgcccgc cgcgccaagg aagccatgag cttttctgac taccggcccg
    20761 gcagcgccac ggcggaatac cggcactatg tcgatcaggc cgtggaaatt gcggagcgcc
    20821 agaaaaagcg tgtggagcca gagtatcacg aaaagataga cagcctcctt gacacctacg
    20881 cccgcaagct ggccgagaac atgaataagg gctatgagat taccgcccgc gtcccctctg
    20941 tcctgattgc cgggccgtcc aatttcccgg tacgggcgaa agaaaagcag aacgccgcca
    21001 gtgaccgcaa catggaggaa tttcagtaca ttcagggctt gcttgacaaa atccgcagca
    21061 ccggcatggg tggtatcagc gccgacgatc cgcaggccgt ttccaagctg gaaaagaaac
    21121 tggaaaagct ggaagcgtcg caggagttga tgaaggccgt caatgcctac taccgcaagc
    21181 atgggacgct ggacggatgc ccccatctga tcgaaagagg cattgaaaac ctgaaagcgg
    21241 atatggcgtc gggctggcac tatgagaaaa aaccgttcca gtcctggcag ctctccaaca
    21301 ataacgccga gatacgccgg ttaaagggcc gcattgagga attgacccgc caaaaggagg
    21361 cggcctatgt gggatgggag tttgacggcg gcactgtgga gatcaaccgg gtggccaacc
    21421 gcctgcaaat cttctttgag gggaagccgg atgctgctgt acgcgacgag ctgaaaagca
    21481 acggtttccg ctggtcgccc aaggccgggg catggcagcg gcagttaaac gacaccacaa
    21541 tccgcgtcgc tgaccgtatc aagtgcatcc agcccctttc cggcgaaaag cccagcgcct
    21601 tacagcttgc ggcccgcagg gagaaagaaa agccctctat ccgggcgcag ctcaacgcgg
    21661 ccaagaccga acaggccaag cagccaccgg cgcgggaaaa atcaaaggga ttggaggtag
    21721 agtaaatgca gaagtttgaa aatgtggacg ttctggccgc gctggaacag atcatgcggc
    21781 agaacaccgc gttttatcaa agtgactttg gcattgacaa agacattatc aggcgggatg
    21841 ccgccagcga tcaggccgca gacaagaccc tgctctggat gtcccggccc tccgggacgt
    21901 actgtttccg ggaacgtgat gttttcctga aagacacccg acagtacaac acatggaaat
    21961 tttacgggga gcagacccgt gacaagatac tggcctacgc ggtggagctg accggcacac
    22021 agggcggcac tatccggggc aatctctatg agctggacta tccgcagcat ttccggcacg
    22081 tcattgaaac cgcccagccg gtgagtgtga accggctctt ttatgagcat ggcacccgtg
    22141 acatcccgga gagccagcgt tttgacggaa gccctgaccg ggtattggga aactttttgt
    22201 actatgaggc ccagccccat gacccggctg tgctgcagga ggcgctacgg acggagcagc
    22261 acagccgggg gcgggccgtc cccggcgatt tcaaagtcca cgtcaccgct ttgcatgaca
    22321 gccggattga aaccgaggcc cggcgtattg tggacagcct gaaagatctg tccgcgccta
    22381 acagccccaa taaaacgcat ttcatggtgg agatctcccc gcactttgcg gcgattgctt
    22441 ccaccaagga taatgaccgg ctctttgcca tgctgcccta taaatccctg tgttttaccg
    22501 ggatgaaagg caggcatggc gtgtatgccg tgattaaccg ggacgagcgc cgggatgtga
    22561 gtattcgcag gccccggccc tctatccgca agcagctatc cgacagcaaa caggccgcca
    22621 gcccaaaaaa ggcggcggcc agaaccaaaa agaatgaatt ggaggtataa ggaatgatgc
    22681 actttaccgt ggaggaagaa aacctgatct gtatgtacca caatgcagac cggcgcagaa
    22741 cgataaacag gctcaccgcc gtcatgccgg ttatggatgg ggatatgcgg gcgcaggccc
    22801 agcagaccct ttccaagctg gaacgcatga cggatgccga ctatgacggc cagaagttcc
    22861 acttcacaga cgaataacga acagcagacg ccgggcgcgg cggccatgtg gtcgcttgcg
    22921 tccggcgtct tttctttttg cctattccta cgcatttaga acccggtatt gtcggggata
    22981 ggatgaagta ttcgcttgcc cctgcaaaca tgcgggagaa gccttgcagc gggcggtttt
    23041 caatccctta aatgcgtagg ttgcagctat aattagaccc ctgaaacaga tatagccgcg
    23101 cagcgggtgt atctgtttca ggggtgtgca ggtgatagcc gcaaagcggc gtaagagggt
    23161 gcagccctct tgccgtctat ctctgttcca gcgggaagct cggcaatgct tccagcagtt
    23221 tcagggaaat gcgctgccgt atatcttcgt caatgacttt cttctttttg ccgtttgcct
    23281 gtctgatctc caccgtggaa agctcgttga tgtagtcagc ataatgggcc aacacttttt
    23341 ccgtggccca cctctcaccg gcaacggcgg ctttgatcgt ttcatagtcc agcagctttt
    23401 cttgtccgtc catcctgata cccccgtcag actgattttt tctatgatac catattcgga
    23461 agcatggaac cagagaaaac ggcgcttttc tgctggtaaa ttctacgcat ttagaacagc
    23521 acttcggcgg ggataggata aaacacccac ataccccgct gaccatgccg gaaaagcctt
    23581 gaaatataag ggcttttaca gctctaagtg cgtaggtttc cagtatcttt tccgctggcg
    23641 ctcggcctct ttgcggcggc gcatccgttt ggagcaggcc gcgcagtatt tggcccggtt
    23701 agacttcggc gtgaacgtgg ccccgcactc acagcagcgt ttcacttcgt cccggtcttt
    23761 ggtgatctcg gcatacaggg ccgcatccag cgggaggaca gcggccttaa accagcggca
    23821 cagaagcgaa taggaaatgc tctgtacgca aatacattct tcgccgtcgt ccagcaacag
    23881 gcagttgccg tcacaatagt tgcagcactc atgcgtcagc ttccgggcct tgcgatattg
    23941 agggtaattc attcgtggaa tactcatagc tcttgcgtcc attcctttcc cggcgtcata
    24001 cgcaaaatgc cgtccacatt ctgcttgatg atgccgtatt cctgcatctg ctttttcgct
    24061 tcgccgtact tctcatacag ccgttccttt tcctcggcca atcgcttgta ctcggctttc
    24121 agggcgtaca gatcggggag ctttttcaca ccctgtccct ttagggcctt tgccgcagct
    24181 tcatacaaaa tcaacgtgct ttcatgctca cgccggaatt ttgccttatc cgctgcattg
    24241 cggtattcca tagccacggg ccagagctgc ttgtaggtag aaatattctt tatcagcacc
    24301 gccatatccg caagccggtg ttccatagct ttgagggtag cggcggcctc gtcgctggcg
    24361 gcggtgatct cggccacttt gctttccaaa tccgggtagg tgtctatccc atgttcagtc
    24421 aggaaattca aagtctttgc ggcctgcttc aaattgtgga gcttcgccca cctctcatag
    24481 cctgccgact gctgggcctt aatgttgttt tccagatcaa tccgcaaaga aatccccttg
    24541 cgctctttgg gcgctctggt gataacgcgg cctttgatac gctctttgat ggcttcctcg
    24601 gtgtaggctt cccccaatgt cttgcagcgg gtaaagcgtt cctgtccggg agcgcggaaa
    24661 gaaataaact ttcccggctt gatctcatag ccctgcgcct gcatgagccg caggaaatca
    24721 tcaaaatctt ttgcctgcgg gatagcggcg tcaatggctg ctttgagctt cgctttccag
    24781 ctcgtccctt tccgctgggc gtcccactcg gcatagctct tgcccttact ctggccgggc
    24841 ttcaccacgg acaatccgtt ttccttgcac agcctgtcgc tggtgcgccg gatataggca
    24901 tagctctgtt tgttggaaat gtacttgcgc tgatttacca tatccacggc acagaaaata
    24961 atgtgattgt ggacgtggcc cttatcaata tgggtagtca gcacataggg atatttccct
    25021 tgcagcactt cctcggcaag ctgcttcccg atctcatggg cctgttccgg cgtggcttcg
    25081 ccgggggcaa aggattgtat caggtggtgg cccagattat tgcccttttg gtatgcctgc
    25141 gccagcagca tttcaaattc aatgtccgct gtttcataag agcaggcaaa ggaggccacc
    25201 agcagcttgc cgtcagtttt gtccgggttc tcgatgtagt caagggcttt tttcagcgtt
    25261 gacttgatag gatgtatctt tgtaactgcc atatctgcgc cagcgccccc tttatatcct
    25321 cgatgtcctg tgcgtacacc ggccccatac cgttcagcct ctttgcaatc tgattgacgt
    25381 tgacgccgat cttctgtatc tcggcggtct gcgcccggat gtcgctggtg tccatgttga
    25441 cgatatagcc gtcaatgagc atcttccggg cgtaggctga aaagttcttc gtatgcagca
    25501 gcgccatttt ctgttcaatc atagcccgtt cctccggcgt cacagggacg cgcagcacga
    25561 tttttcgctt tctgtttgcc atgcttcacc gtcctttcca ttcaggggtt tggggatttc
    25621 cccaacaagc aaaacggacg cggcccggca gggacggggc aggccgtttt tccgggagtg
    25681 gctacactcc ctatgcttgc tacgttactg tttctccact aacaagacgt ttccaaagcc
    25741 ccaaactacc cgcaaaatag ccctctggaa agaaaaaagt gcaaaaaaat agaagctacg
    25801 ctgtgcagct cccgctaaaa gtaaaacaga ggatgccagt tacacatcct ccgtttcatt
    25861 cttcttcggc ttttcaatta gtatggcgct gtcaatcgcg ccctcgatga tcggcaggta
    25921 ttcttcgggg cagagtttca gcttgtggct gaccctttgc cgcagttcgc tttcctcccg
    25981 catgatctcc gggttaaagt agcgttccac aggaagcccg caggctttaa taagctgtat
    26041 gacaaccggc agactgggaa tggcccgtcg ttttcgatgt tggcaagata gcgccagtca
    26101 atatttattt tctctgccat tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc
    26161 acatccgcgc cgaaagtttc aaagccggga caatcttcaa tattcgccat atcgcacctc
    26221 ccacttacat tgtatatttc atactctgcc cgtggaatgt tggtatatgc acctttctta
    26281 aagtttatat ttcacatttt gtttctttta ttcttcgagc aaaagaatta taatgtaccc
    26341 tgtggaggtg tactatggac tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc
    26401 ccccacgatg gataaactac tattgtgctg cgggacgcat ccccggcgct gtaaagatgg
    26461 cgacgatctg gctcattccc aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag
    26521 gaaaggcggt tactgaatag tataatgaaa ccggcgcatt ttggatatgg ttttgaagag
    26581 agagaaagca tggtattttg tcacggaaaa actataagat gatagatggc aggctgctcc
    26641 agaccgataa aaagtattct catctgaaac agaaagaaaa gatttacaca tggatgtatg
    26701 aagaagcgaa actttatcat gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct
    26761 gctgctgtat cagccgtata tggtagaatt gaaaaggccg gcatctggat tctttatggt
    26821 gaagtctcca gacattggat aactgctggt ctaatgcgcc tttgagtatg gggtgattga
    26881 ttttaatacc ggggaggtac ggctttttga ggaaaatatt ctgggattta catttggcga
    26941 ttattacatt tcctgtaact gcaaggacac ggatgatgaa gatatgagga atatagactt
    27001 gctgattttc tactgtcttg aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa
    27061 gagcgaccgg agggagtaca cacaggccct gcctgtgtga aaagccatac cgcaaggtgc
    27121 attcgcaaaa aagaggccgc gcattttgct gaggcttggg gtgtccccaa caagcaaaac
    27181 gagaacgccg caggcgggca agtggtggat cagaatatcc gtaaaggata ttctgatcca
    27241 ttgcttgcca gtattatcgg gatgttttga caaccgctgt aatcgaaaca tcccaaacat
    27301 taagaaggtg ttaattatta ttatcttgat ttaattcgtt taaaccaata atatagatac
    27361 atacaccatg aaaagaagcg agtatcccaa tgacggatga ttaaaatccg aaaagaaatc
    27421 gctttgaaac tacagggaaa ctacagactg ttatgttatc ttcttaaaat ggagggattt
    27481 ttatgtcgat acgaattcta cttgtcgagg atgatgatca tatctgtaat acagtaaggg
    27541 cgtttctggc tgaggcagga tatcaggtgg atgcctgcac agatggaaat gaggcataca
    27601 ccaagtttta cgaaaacact tatcaactgg ttattcttga tattatgctg cccggtatga
    27661 acgggcatga acttttgcgt gaatttcgtg cgaaaaatga tactcccatt ctgatgatga
    27721 cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga tgcagaggca gacgactatg
    27781 taacaaagcc attcaagatg cagattttac taaagcgggt ggaagccctg ctgcggcgca
    27841 gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac acttctgccg gaggatttca
    27901 cggtactttg tgacggtaca gagctgcccc tgacacgaaa agaatttgaa atccttttgc
    27961 tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat cattttgtca cgtatatggg
    28021 gatatgactt tgagggtgat ggcagcacag tccacactca tatcaaaaat ttgcgggcca
    28081 agctgccgga aaatatcatc aaaaccattc gcggtgtagg ataccgattg gaggaatcat
    28141 aataatggaa agaaaaggga ttttcattaa ggttttttcc tatacgatca ttgtcctgct
    28201 actgcttgtc ggtgtaacag caacactgtt tgcacagcaa tttgtgtctt atttcagagt
    28261 gatggaatta cagcaaacag taaaatccta tcagccattg gtggaactga ttcagaatag
    28321 cgataggctt gatattcaag aggtggcagg gctgtttcac tacaataacc aatcctttga
    28381 gttttatatt gaagataaag agggaagcgt actctatgcc acaccaaatg ccaatacatc
    28441 aaatagtttt agacccaact ttctttatgt ggtacataga gatgataata tttcgattgt
    28501 tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg ctgacaattc ggggaattgt
    28561 tatgattgcg ataatggttg tattcagcct tttatgcgcg tatatctttg cgcggcaaat
    28621 gacaacgccg atcaaagcct tagcggacag tgcgaataaa atggcaaacc tgaaagatgt
    28681 accgccgccg ctggagcgaa aggatgagct tggcgcactg gctcatgata tgcattccat
    28741 gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat gaaatcgcaa gggaacatga
    28801 gttggaggaa acacagcgat atttctttgc ggcagcttct catgagctaa aaacgcccat
    28861 cgcggctaca agcgttctgt tggagggaat gcttgaaaat atcggtgact acaaagatca
    28921 ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg cagggcaaaa tcatttccga
    28981 aatactggag cttgtcagcc tgaatgatgg gagaatcgta cccatagctg aaccgttgga
    29041 catagggcgc acggttgccg agttgctgcc cgattttcaa accttggcag aggcaaacaa
    29101 ccagcggttc gtcacagata ttccagccgg gcaaattgtc ctgtccgatc cgaggctgct
    29161 ccaaaaggca ctatccaatg tcatattgaa tgcagttcag aacacgccgc agggaggcga
    29221 ggtacggata tggagtgagc ctggtgctga aaaatgccgc ctttttgttt tgaacatggg
    29281 cgttcacatt gatgatactg cgcttccaag gctgttcacc ccattctatc gcattgatca
    29341 ggcgcgaagc agaaaaagtg ggcgaagcag aaaaagtggg cgaagcggtt taggacttgc
    29401 catcgtacaa aaaacgctgg atgccatgag cctccagtat gcgctggaaa acacctcgga
    29461 tggcgttttg ttctggctgg atttaccgct cacatcaaca ttgtaaatat ttaaaactta
    29521 aatgattttg ggctctttgt caaataggac tgatagagtg agataagaac atctaaggta
    29581 gaattccttt tctactttag atgttttttg attcataggt aatcagatta tttagtaaga
    29641 aaattctggt cttcattttc cgaaagcttc gaaagccata agcgactcgt ttcaatgctt
    29701 tgatgtgggt atgcagattt tctattttgg catttgaata aggcaattcg agggctaaac
    29761 gaatgccgga ttcatagttt ataaaagctt tttttataga atgcagttct tttggaacgg
    29821 aattgggcat cgttcttaca agtttaaaga aagtgtccgc ctctttgtcg tgaaacgctt
    29881 gaagaatttc ttgatagtaa aaataggcga ttcgtaattc gtcagagatc gaaagcagac
    29941 gatccaccac ttcactttcg gtgagatacg cccaaccaaa attgcgccat ttggtccatt
    30001 tgtcataaac aagcttgtcc tgtcgtttga tcagaagccg agacaacgcc tttaattgtc
    30061 gataatattt ggcttgtgtt ggatcgtttc gatcaaagct tttcatgatt cgtacgcgaa
    30121 tatcctcaaa tttacgaatg agatgtttga caatgtgaaa acgatccaca acgagttttg
    30181 cctttggaaa acattccttt atcagagagg cataagaaac attcatgtcc gtcacgatga
    30241 cctttacctt caatcgtgcg cgtcgttcaa attgcataaa ataagaaacg agagcctttt
    30301 tcttccgtga aggaagaata tccagtaatt ttccagtatc gccatccata caagaaaagg
    30361 agtagggacc gacttgattt ttcagtgaac gaaattcatc cattaataaa caacgcggta
    30421 accaatttct ttttactttt gtcatgggga tcagagaagc gagtgtccga gaaacggtcg
    30481 accaagaaac gccgcaatgc ttagcaattg tcgtcattgc ttgtttttca gttagctctt
    30541 ctaaaatttt atagcgcact tgtttagcga taaagcagtt tctatcgaca atgtaggtat
    30601 tcgccgtaaa ataggtgtgg cagccacgac agtggtagcg ttgtttcgct aaagcaagat
    30661 aagtcagttg gttcgcacat ttattaagtt gaatgagcga ttttttcttt ccgttcttaa
    30721 cgatcatgcc ttcatgattc atctgtttac acaaaggaca gcgacgaatt tctttactta
    30781 acgtcgcaga aacaacaaga ctcttgatat tcttaatgct cttcgattcc acatttgtga
    30841 tgaaaatgtt tttatctttg attccaagta gtagtttgat atgatgaaga tcaggcatac
    30901 agaacgctcc tttgattgtg ttgtggtgat tcaattctaa cggagcttct gtatgttttg
    30961 ttttttcatg ctcaaaaggg accgatagaa tgattttcta tc
//
LOCUS       DADVXO010000018        30807 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.018, whole genome shotgun sequence.
ACCESSION   DADVXO010000018 DADVXO010000000
VERSION     DADVXO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.018"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(153..317)
                     /locus_tag="KB950_000716"
     CDS             complement(153..317)
                     /locus_tag="KB950_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856952.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            612..1940
                     /locus_tag="KB950_000717"
     CDS             612..1940
                     /locus_tag="KB950_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBB6856953.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2142..2975
                     /locus_tag="KB950_000718"
     CDS             2142..2975
                     /locus_tag="KB950_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBB6856954.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2991..3728
                     /locus_tag="KB950_000719"
     CDS             2991..3728
                     /locus_tag="KB950_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBB6856955.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3883..4851)
                     /locus_tag="KB950_000720"
     CDS             complement(3883..4851)
                     /locus_tag="KB950_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBB6856956.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5083..5916
                     /locus_tag="KB950_000721"
     CDS             5083..5916
                     /locus_tag="KB950_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBB6856957.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5950..6789
                     /locus_tag="KB950_000722"
     CDS             5950..6789
                     /locus_tag="KB950_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6856958.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6815..7375
                     /locus_tag="KB950_000723"
     CDS             6815..7375
                     /locus_tag="KB950_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6856959.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7574..9295
                     /locus_tag="KB950_000724"
     CDS             7574..9295
                     /locus_tag="KB950_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBB6856960.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9460..10602
                     /locus_tag="KB950_000725"
     CDS             9460..10602
                     /locus_tag="KB950_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6856961.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10618..11829
                     /gene="thiI"
                     /locus_tag="KB950_000726"
     CDS             10618..11829
                     /gene="thiI"
                     /locus_tag="KB950_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBB6856962.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12402..13049
                     /locus_tag="KB950_000727"
     CDS             12402..13049
                     /locus_tag="KB950_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBB6856963.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13178..13394
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13442..16087
                     /locus_tag="KB950_000728"
     CDS             13442..16087
                     /locus_tag="KB950_000728"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBB6856964.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16397..17710
                     /locus_tag="KB950_000729"
     CDS             16397..17710
                     /locus_tag="KB950_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBB6856965.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17700..18353
                     /locus_tag="KB950_000730"
     CDS             17700..18353
                     /locus_tag="KB950_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6856966.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18408..19100
                     /gene="radC"
                     /locus_tag="KB950_000731"
     CDS             18408..19100
                     /gene="radC"
                     /locus_tag="KB950_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBB6856967.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19250..19450
                     /locus_tag="KB950_000732"
     CDS             19250..19450
                     /locus_tag="KB950_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6856968.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19704..21383)
                     /locus_tag="KB950_000733"
     CDS             complement(19704..21383)
                     /locus_tag="KB950_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBB6856969.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21385..21597)
                     /locus_tag="KB950_000734"
     CDS             complement(21385..21597)
                     /locus_tag="KB950_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBB6856970.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21990..23720
                     /gene="efrA"
                     /locus_tag="KB950_000735"
     CDS             21990..23720
                     /gene="efrA"
                     /locus_tag="KB950_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBB6856971.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23717..25477
                     /gene="efrB"
                     /locus_tag="KB950_000736"
     CDS             23717..25477
                     /gene="efrB"
                     /locus_tag="KB950_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBB6856972.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25572..25769
                     /locus_tag="KB950_000737"
     CDS             25572..25769
                     /locus_tag="KB950_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856973.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25922..26407
                     /locus_tag="KB950_000738"
     CDS             25922..26407
                     /locus_tag="KB950_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBB6856974.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26511..27104)
                     /gene="clpP"
                     /locus_tag="KB950_000739"
     CDS             complement(26511..27104)
                     /gene="clpP"
                     /locus_tag="KB950_000739"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBB6856975.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27284..27811
                     /locus_tag="KB950_000740"
     CDS             27284..27811
                     /locus_tag="KB950_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6856976.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27902..28639
                     /locus_tag="KB950_000741"
     CDS             27902..28639
                     /locus_tag="KB950_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB6856977.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28643..29560
                     /gene="miaA"
                     /locus_tag="KB950_000742"
     CDS             28643..29560
                     /gene="miaA"
                     /locus_tag="KB950_000742"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBB6856978.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29562..30791
                     /gene="hflX"
                     /locus_tag="KB950_000743"
     CDS             29562..30791
                     /gene="hflX"
                     /locus_tag="KB950_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBB6856979.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10173 a         5271 c         6573 g         8790 t
ORIGIN      
        1 gtcctactgc cggagtaaat aaagcttatg caaagaaaaa gaaatccaat cacttgggtt
       61 tctttttctt ttattattat gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat
      121 ctttacgaaa gagtagagtt ggctactcat aatcaaccat acttcccatc atcgctaaat
      181 acgaaataaa tcagcattcc tacaccaatc aaagcacaag ctgcaccaag aaaagggatg
      241 acattcccaa agttatctgt attcataagt atgccaccac ccacaaacaa aaagccgcca
      301 gcaatcaggt ttttcatttt attcatctcc tttcttacgt tatagtattt ccttctttca
      361 atgagtattt tgaaagctat cttattgtat gatgttcttc taggcctctt ctttattata
      421 caggccattt tcgttatctc atagaaatat tcctttttat ctgaaaacta ttgtataccg
      481 tatgacattt ttcttcatga agttgtaagt atattctcat tcaaatgaaa atttcatcat
      541 agaagagatt gtatttctaa attcttaaga gtaaaatacg aagtataact tggatttgga
      601 aggatgattg gatgaaagct attacgcctg atatgacaac aaaatttaac aaacagtttc
      661 aagcaaatat caaacagcag gcattgcagc gggcagttgt gaagaacgga attactgctt
      721 ctgctgaaaa tgtttcagca catgtggaga atacaccggt attttctata gatttggcta
      781 cggggaaagt ggctaatcaa atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca
      841 cattccgtca taagatgctg aatcaattcc atttaaaaga gtttgaatta tcacaaaatt
      901 acactttttt ctgggataaa tatgaaaagg caaattattt ctatgaaaac atcattgcaa
      961 caggaaaaga ggcaatcgat agccggaaag tagctttctt actcgctact cctcaacaag
     1021 atggcggcca atgggatatg atcgtttcat tattccaaaa atatggcgtt gtgcctaaat
     1081 cggtgatgcc ggaaagtgcg aatagttcaa attctcgaga tttgaataac tacttgaaca
     1141 aattactgcg taaagatgca gtagtgttac ggaaaatggt tgctcaagga gaaacattag
     1201 acgctattga agaaaagaaa gaagaaatgc tagaaagcat ttataacttc ttagctatct
     1261 cattaggaac acctccaaaa gaattcgatt ttgagtaccg cgatgaagaa aagaactatc
     1321 atcttgaccg aggactgaca ccacaaattt tttatgataa atacatcggt gtaaaattag
     1381 atgattatgt tagcgtgatc aatgcaccaa caaaagacaa accattcaat cgttcttata
     1441 cagtagagat gctaggaaat gtcgttggcg ggaaagaagt caaatatctg aatgttgata
     1501 tggaaacttt caaaaaatta gcgattgctc agcttgaaga aggagaatct gtttggtttg
     1561 gttgtgatgt aggtcaatct tctactagaa ctagtggcat catggcctta gatgcttatt
     1621 caatggatga tctatttgat acggatttta caatgactaa agctgaacgt ttagattttg
     1681 gagaaagtct gatgacccat gcgatggtat tgaccggtgt tgacttagta gacgggcaat
     1741 caacaaaatg gaaagtcgaa aatagctggg gcgaaaaagt tggaaagaat ggcttctttg
     1801 ttatgagtga tgcctggatg gacgaatata cgtaccaaat cgttgtaaga aaagatttat
     1861 tgacgaaaga gcaattaaat gcgttcaatg aagaaccaat tgttctatct ccgtgggatc
     1921 caatgggtgc tttagcatag acgagtattt caatgaagag tatgtagtaa agataaaaag
     1981 agctggctta agccagctct ttttatcttt aaagtccatt ccctgttttt tatggtccct
     2041 gtgctataat tctaaataaa ggataaggat tggaaataat ccttttatca ttaaaaatat
     2101 gatgacgaag tagaataaaa ttgggagtag gggggaaagt catggatatc caaactgttt
     2161 tatctgatct gaaattaaat ggtaaagtga ttccagttgt tggtggcgat gttaatcaga
     2221 cctatcgtat aaaaaccgaa catcgtgctt attttttaaa aatccatccg aatgtaaaaa
     2281 aaggattttt tgaagcagaa gtagacggat taaaagaact gtcagcattc gtacgtgtac
     2341 cggatacgta tatgctaggt gaaacaagtg aaggcgctta tttgttaatg gaatggatcg
     2401 agccgggaaa aggggatcaa cgagatcttg ctgcagcttt agctaatctc catcagcaaa
     2461 cagcgccaca atttggtttt cgtaaagata actatttggg tacacttgtt cagaaaaatt
     2521 cttttgaaga agattggtgg acttttttct ttaaagatcg gcttgaatcc cagatttctt
     2581 tggcagaaga aacgaatcga tggaatgtac agcgtcaaga aaagtatctg cgatttaaag
     2641 aacgtgtatt gaaatcggtt gaaccaaaaa agatcactcc acgcctcttg cacggagacc
     2701 tgtggagtgg taatgtgttc tttgaccagc aagggcatcc agtttttgtc gacccagctg
     2761 tttcttatgg gaatcgggaa caagatattg caatgagcca gttattcggt ggtttccgac
     2821 cggaattttt agatgcttat caaactatat ttccattaga aaagggatgg aaagaccgac
     2881 tgccgattta ccagttatat tatcttttgg cacatttgaa tatgtttgga gaaagttacg
     2941 gttcccaagt agatcaatta ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa
     3001 aatataccaa aaagcatgaa gtttcatatt atgagtgtga catcaaccaa acgatgacat
     3061 ttccttcgat gttaggagta gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa
     3121 gaggcagtga attcgtcaat agctttggac ttacttgggt catcacgaat tattccatgg
     3181 agattaatcg tctccctaga gtcggtgaag taatctcagt aacgactcaa gccatggagt
     3241 acaataagtt tttctgttat cggaacttct ggatccatga tgaagatggg aaagaattag
     3301 tcaaaataga atctgttttc gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta
     3361 atgaagagat cattgctcct tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa
     3421 aaattgaaaa gattgattcg ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg
     3481 atagtaatca gcatgttaat aatgcgatgt attttaattg gatcattgac gtattaggat
     3541 atgacttttt gactacgcat gttccagaaa aagtaattat tcgatttgat aaagaagtag
     3601 agtacgggaa tgagatcgaa agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa
     3661 caaaacatga aatccgaatg caagaacagc tgtattgtga agcaaatatt atatggaaaa
     3721 ataaataaaa aagaggttgt gacagaaggg ggcaacttca agaaataaga agaaatttac
     3781 gaaaattgtt tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa gctatttttg
     3841 tcacagcctc tctttttaag tttagctatt agataatgat gtttagtttt ggataaacga
     3901 aatcaattct tcacccgaca aaccatagtt tacagcaatc aatagtttta aacgtgcttt
     3961 ttgactatta atactgttac agaaaataat ccccattttt tctagttctt ttcccccgcc
     4021 ttcataatca taaacaggct ctgcgattcc attgaaacaa cgagaaacaa gtactacagg
     4081 gattttttgg acaagaagct tttccaaagc agctaaggct tgtggaggga gattccccgc
     4141 acctaaagct tcgattacca aaccgtctac tttcgtatgg gcgatggctt ccaataagtc
     4201 cccttgcatc ccagcatatg ctttaacgat cgggatagtc ccatctacag cggaaatgtc
     4261 cagtcgcttt gtttctaaaa gttcctgcaa gaataaaatt cgattcttag ttactaagcc
     4321 gatgggaccc aatgtaggtg ttcggaaagt agcaacattt gtggtatggg tctttgtcac
     4381 gtagcgtgca gaatggatct catcgttcat gaccaccaaa acccccttat ctagcgcttc
     4441 ttcacaactt gcgacacgaa tcgcactttc aaaattataa aggccgtcac ttccaagttc
     4501 attacttgaa cgcattgccc cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc
     4561 aagaaaaaaa gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat
     4621 acctgatgca taagcggaca agatacgttc ttttaatacc aacatgtgag aaggcatgat
     4681 gtgtggtgag ggaagattga agatatcttc aactaataat tctacatgct tggggattgt
     4741 gatttctgca tctaataatg ggtttgccac atcaggacta acaccgcccg tgagcatgtt
     4801 ttctttcatg gcgatcgttc cgccggtgtg tagtacaagt atttttttca ttttacagac
     4861 ttccttacga ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt
     4921 tgctatcagt gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa
     4981 atggaaaaga ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta
     5041 caagaaagca gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta
     5101 acatcgttta aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat
     5161 gcagggtttc tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc
     5221 tcagttgaga attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat
     5281 caaggaatca gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt
     5341 gcgcattttt tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta
     5401 gtccaattcc cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt
     5461 agaaagtggt ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc
     5521 gatgaatttc ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc
     5581 atcgatgagc cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg
     5641 aactttgctc tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa
     5701 gagtggcgta aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca
     5761 catgaagtca aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat
     5821 tctggtggtg atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca
     5881 gatggactca atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg
     5941 gcgaagtcta tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt
     6001 tcaaaagcgt tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta
     6061 tgcggtgtag caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa
     6121 ttagatatgt atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc
     6181 gaccaccctt ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat
     6241 cgacaaatcg tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga
     6301 caatcgattt ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg
     6361 atgaaaaaaa tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta
     6421 gagatattaa aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa
     6481 aagttagaac atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat
     6541 atcatcccaa aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa
     6601 attgaaatgg aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa
     6661 ggtgtaggaa ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac
     6721 tttgtgactc aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg
     6781 atttattagt caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa
     6841 agtagaaaca ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac
     6901 agaaaatgaa gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc
     6961 agctacttca cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt
     7021 agataaagca gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga
     7081 acaagtagat gcactgacag atatccttta ctttacttat ggttcttttt cattactagg
     7141 aatagtgcct gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc
     7201 agatggaaaa cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca
     7261 gatggaatat gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc
     7321 gattgctaag aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga
     7381 gtttcactat ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag
     7441 tgaatgcatg aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta
     7501 ttatttggat ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac
     7561 ggaggaacca taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt
     7621 cgccattgtc gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga
     7681 tgccttagaa aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga
     7741 tgttaaaaaa atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa
     7801 atggaatgac atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga
     7861 aaacctcaat gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc
     7921 tacaatggac caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg
     7981 ggaaacggaa gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact
     8041 gaaagaaaaa gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa
     8101 acagatcaaa gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga
     8161 ccctgttgaa gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc
     8221 tctgatgaaa aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact
     8281 tgaagaaatc agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga
     8341 agatcttgct gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct
     8401 tgaaaaaaca gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga
     8461 ttcgttatat gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca
     8521 atcaacgatt gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga
     8581 gctggatcac acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg
     8641 tttccaaacc gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg
     8701 tcaacacgaa attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt
     8761 agaagatatc gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg
     8821 cgaaaaagaa gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg
     8881 ttttgtagaa aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt
     8941 tgctactgac cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga
     9001 agaggtcaat cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac
     9061 acatgattta gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta
     9121 tcgtcataca cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa
     9181 agaatatcac tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc
     9241 tggtgctttc aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa
     9301 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
     9361 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
     9421 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
     9481 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
     9541 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
     9601 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
     9661 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
     9721 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
     9781 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
     9841 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
     9901 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
     9961 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
    10021 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
    10081 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
    10141 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
    10201 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
    10261 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
    10321 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
    10381 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
    10441 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
    10501 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
    10561 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
    10621 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
    10681 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
    10741 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
    10801 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
    10861 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
    10921 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
    10981 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
    11041 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
    11101 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
    11161 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
    11221 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
    11281 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
    11341 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
    11401 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
    11461 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
    11521 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
    11581 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
    11641 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
    11701 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
    11761 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
    11821 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
    11881 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
    11941 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
    12001 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
    12061 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
    12121 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
    12181 atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
    12241 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
    12301 tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat
    12361 gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata
    12421 cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg aagttgctgg
    12481 atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt
    12541 ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct
    12601 atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac
    12661 gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa
    12721 gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag
    12781 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
    12841 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
    12901 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
    12961 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
    13021 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
    13081 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
    13141 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
    13201 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
    13261 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
    13321 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
    13381 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
    13441 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
    13501 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
    13561 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
    13621 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
    13681 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
    13741 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
    13801 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
    13861 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
    13921 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
    13981 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
    14041 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
    14101 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
    14161 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
    14221 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
    14281 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
    14341 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
    14401 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
    14461 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
    14521 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
    14581 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
    14641 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
    14701 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
    14761 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
    14821 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
    14881 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
    14941 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
    15001 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
    15061 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
    15121 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
    15181 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
    15241 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
    15301 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
    15361 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
    15421 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
    15481 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
    15541 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
    15601 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
    15661 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
    15721 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
    15781 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
    15841 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
    15901 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
    15961 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
    16021 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
    16081 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
    16141 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
    16201 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
    16261 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
    16321 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
    16381 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
    16441 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
    16501 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
    16561 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
    16621 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
    16681 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
    16741 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
    16801 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
    16861 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
    16921 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
    16981 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
    17041 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
    17101 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
    17161 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
    17221 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
    17281 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
    17341 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
    17401 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
    17461 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
    17521 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
    17581 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
    17641 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
    17701 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
    17761 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
    17821 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
    17881 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
    17941 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
    18001 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
    18061 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
    18121 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
    18181 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
    18241 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
    18301 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
    18361 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
    18421 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
    18481 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
    18541 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
    18601 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
    18661 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
    18721 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
    18781 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
    18841 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
    18901 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttattc
    18961 catcgcagca ggatatgaat tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga
    19021 tcactgtact ggaccacttg atcatcggac gcaaacgata ttttagtttg agagaagaag
    19081 gaatgatgga agagaagtaa gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta
    19141 tattgtaatt ttgtttggga atagaagttg aaacatctgt tgcacctcta tcgtacacga
    19201 gcaatgttac aatttaaatt agtgcttttg gcacgaggag gatattatta tggaaaacgg
    19261 aacagtaaaa tggttcaacg ctgaaaaagg atttggattt atctcacgtg aagatggaag
    19321 cgacgtattc gttcacttct cagctatcca aggtgacgga ttcaaaacat tagaagaagg
    19381 tcaagcagtg actttcgacg ttgaagaatc agaccgcggc ccacaagctg caaacgttgt
    19441 aaaagcttaa ttttatctgt aaatcaaatt gatggtagac atcaattttt acgagagaac
    19501 aaagcgagta acaaaaatac ctgccacaag agacaatttc tcttgtggca ggtatttttt
    19561 tgtatgaaaa agtaaataaa tttaagaggc tatgaaagag ccaccttgta tcaaggaata
    19621 agaacaaaca aaaatggctt ctcatgtttt tcatttgttt tctcttatat tcaggacaag
    19681 gtgctgattt cataacctct tatttattct tcatctggtg tcattaccat tggcaaaatc
    19741 attggatgtc tttctgtacg ttcgaataag aaaggctgaa gcgcgccagt catcgcttct
    19801 ttcaatttat attctgaaca gtcaggtcca ttcaaacatt ctcttaaagc gtggaataag
    19861 attcgctgag cttcattgat catttctcct gattctcgca tgtagacaaa tccacgagag
    19921 agaatatctg gaccagccaa gatttctttt ttcttgatat ccactgtcgc gacagctaat
    19981 acaagacctt cttctgaaag caggtgacgg tcacgtaaaa cgacattacc aatatcaccg
    20041 atcccgctac catcgatata cacatctcca gccacaaaat gcccagcagg acgcgcagaa
    20101 tcgctagtta acgctaaaac atcgccattt tccatgatga agcagttttc tgcaggaaca
    20161 cctacatctt gtgctagaga agcatgtact ttcaacatgc ggaattctcc gtggacagga
    20221 ataaaatatt ttggcttcat caaacgaagc attaattttt gttcttcttg tccaccgtga
    20281 ccggaagtat ggatgttatt gatttttccg tgaatcactt ctgcaccagc ttcagacagc
    20341 aaattgatca aatgattgac acttgttgta ttaccaggaa taggtgaact tgaaaagatc
    20401 actgtatcgt ctggctggat ttggatttga cggtgagtac cattcgcgat gcggctcaac
    20461 gcggccatcg cttctccttg agaacctgta cataggatca tcgtttcatt ggcaggcaag
    20521 cgattgattt cgtgtgcatc tacaaatgtg ccgtctggaa ctttgatata tcctaaacgt
    20581 tgaccgttga cgatggcatt ttccatgctt cgtccgaaaa cagcgatttt acgaccagtt
    20641 gccacagcgg catctgctgc ttgctgcaaa cggaagatgt tagaagcaaa gctggcaaaa
    20701 atgatacgtc cttcgatttt tgaaaagatc ttcaaaatag aatgcccaat cgttttttct
    20761 gatttagtaa atgttggaat ctcagcattt gtactatcag ataaaaggca caagactcct
    20821 tcttccccga tacgagccat tttatgcaag tttgctggtt cgccaacagg agtgaaatcg
    20881 aatttgaagt ctccagtcgc tacgatattt ccagaaggtg ttttcaccac gatccctaat
    20941 gcatctggga tactatgggt cgttctgaaa aagctgacag aagtctttct aaaacggatg
    21001 atcgtatctt cattgatctc atggagctct gcatctcgaa gcaagccatg ctcatctaat
    21061 ttgtttgtga tcaacgctaa tgcgagagga ccagcataga taggaatatt tgcttgtttc
    21121 agcaagtagg gtattccgcc gatgtggtct tcgtgcccgt gagtaatcac caaggctttt
    21181 actttgtgta agttttgtat gatgtagctg tagtcgggaa tgacatagtc aattccaagt
    21241 aaatcgtctt ccgggaattt gatcccggca tcaatcaaaa tgatttcatc ttggaattgt
    21301 accccatacg tatttttccc gatttcacct aatccaccaa tagcaaagac gcctgtctcg
    21361 ttatttttga tggaaagttt catattaaaa ctccactaat gagaaatcag cattttcttt
    21421 ttcgtactct agatgacttc cttctagaag ttgaacgaat tcaatattgt atggggtatt
    21481 ttcttccaca cgttggcgag cgtctacatc gctttttgcc tcaatgtaaa gagattttgt
    21541 ttcttcccgt ttagggttgc gtacttttgt ttcttgataa taaactttat agatcattgt
    21601 tttgtctcct ttatctgcgt aaagcagtga tttagctagt tgattttttt agacaacaca
    21661 aaacgagggg acaagccacc tggtttttca tatgctttga ctagaatact ggattcgagt
    21721 caaagagaca agcctcgttt ttgcttatct gttcggatat tttttcaacc gtaccttctg
    21781 tataaaaaat atacaacaat taattgttat tctagcataa aacagcaaat aagtatagaa
    21841 atcaactaga ttgaaagaaa aaatcaatgt ttatcatccc gctataatca taatgacttt
    21901 tttatgagag aagtgttcta ttaataggct cgttgtgcta tcatttttag taatgaaaat
    21961 gtaacaaaag attaaaagga gatgagaaag tgaaactaat gtggcattat accatgcgtt
    22021 ataagaaatt cttgtttttc aatttcatat gcgtatttgg ttttattttg attgaattag
    22081 gacttcctac gattttagca cgtatgatcg atgtaggaat ccgcaatgat gattttgatt
    22141 atgtgaagca acaagggata tttatgatcg ttattactgt aatcggtatt attttgaata
    22201 tcttgttagg ttattttggt tcgaggatta cgactaatat cgtagcagat attcgagatg
    22261 atttgttcga aaagatccaa agttattcgc atcaggaata cgaaaatcta ggcgtatctt
    22321 cattgatcac tcgtacaaca aacgatgctt atcagatcat gttgttcctg caaaatattt
    22381 tacgtatcgg ttttatggca ccaatgatgt tcattgtcag tttgtacatg gtcatgcgta
    22441 caagtgtgac actcagtttg tatgtagtag gtgcattacc tttactatta ttggcagtcg
    22501 tagcgattgc taaggtgtct gaaccgttat ctaaaaagca acaaaaaaac ttagataaaa
    22561 tcaacagtat cttgcgggaa aatctttcag gattgcgtgt gattcgtgca ttcgtcaatg
    22621 aaaagtttga agaaaaacgt tttgctgtag tcaatgagaa ttatgcttct agttcaaaaa
    22681 gcttattccg tttgatggct gttgcacaac cgggttttta tttcttattt aatatcgtaa
    22741 tggtattgat catctggagc ggggcagtgc aaatcgatgc aggcgagctt cgtgtaggag
    22801 atttgattgc gtttatcgag tatatattcc atgccttgtt ctcattcatg ttatttgcta
    22861 gtgtgttcat gatgtatcca agagcagctg tttcagctgg acgtatccaa gaagccctag
    22921 atgccaaacc ttcgattgta gaagatccaa atggtatatc tgatacaaaa actaaagggt
    22981 atatcgaatt tcaaaatgta acatttgctt atccaggcca ttcgcaagag ccagtgatac
    23041 gaaatgtcag ttttacagcc tctccaggag agaccgttgc ttttattgga agtaccggta
    23101 gcggaaaatc tacgttgatc caattgatcc ctcgctttta tgatgtgagt gaaggaaaga
    23161 tcttgatcga tggagtagac gtgagagatt atcggctaag caagttgcga gataagattg
    23221 ggtttattcc gcaaaaagcg ctgctattta ctggaacgat tgcagataat ttgcgatacg
    23281 ggaaagaaga tgctacgcaa gaagaaatgg aacgtgcagc tgagattgct caagcttcag
    23341 aattcatttc tcgaaaacca gatggattta atgaacattt atcagaaggt ggttccaatt
    23401 tttcaggtgg acaaaaacaa cgattggcaa tagccagagc catcatacgc cgtccggaaa
    23461 tctatatttt tgatgacagc ttttctgcat tagattatca aacggatgcg aagttacgtg
    23521 ctcgtctgaa aaaagaaacg acagaatcta ctgtattgat cgtagcacaa cgtgtgggaa
    23581 cgatcatgca tgcagataag atcatcgtat taaatgaagg agaagtcgtc ggtatgggga
    23641 cgcatcggga gttgttggaa aaatgcccga tttattatga tattgccgct tctcaattat
    23701 caaaggagga attagcatga aaaaaacctt ttcttcctta aaacgtttag ctcgatatat
    23761 cagaccgtat cgtgagactt ttcttttagt gatcctattt acagttctga cagtggcttt
    23821 caacgtggct ttgccatatg ttacaggact tcctactaca gaaatcagcc gtaatattgc
    23881 caatggcgaa tcaatcaatt tttcttatat attcaaatgt ctcatttgga tcacagttgt
    23941 tggaataggg tattgtattt cgcaattact ttctggtgtt ttgatgacaa atgtcgttca
    24001 aagtgccatg catgatttga gaagagacat agaagaaaaa atcaatcgtc ttccggtttc
    24061 atatttcgat aaaaaccagc aaggaaatat tttgtctcgt gtgacgaatg atgtagatgc
    24121 agtcagcggt gctttacaac aagcttttat tggtatcgta aatgcgatat tgggaattac
    24181 gttggctgca gcaatgatgt tttatatcca gccgatgatg gcgctgatct ctatgatcat
    24241 gattccaaca tcgatctgga tctcaaaacg agtgatcaat gcttcgcaga aatattttca
    24301 aagcatgcag aattctcttg gtgagctaaa cggctacgtc caagaaaata tgacaggttt
    24361 tagtgtattg aaagtatatg gacgcgaaaa agaaacattg gaaggattca aaaaagttaa
    24421 tcacagcttg aaatactttg ggtttcgtgc agcattcatt tctggcttga tgatgcctct
    24481 agttcaatta actgcctatg ctacttatat tggtatggct gttctaggaa gcttttatgt
    24541 tattactggg gcgatcgttg tcggtcaact acaagcattc atccaatata tttggcaaat
    24601 cagtcagcca atggggaacg tgacacaatt gtcttcagtt ttgcaaagtg catcagcggc
    24661 aacgaaacgt gtattcgaga ttttagatga gccagaagaa aaagaaaaca aagtagatgc
    24721 ttcactgcca gaaacgattt taggagatgt gacattcgat catgtcgatt ttgcatatga
    24781 tccgaagaaa ccattaatcc aagatttgaa ttttgaagtg aaagctggtc aaaccgtagc
    24841 tgtggtcggt ccgaccggtg ccggcaagac gacattgatc aatctattga tgcgttttta
    24901 tgacgtagac aaaggagcaa taaggattga tggaatcgac accaaatcaa tgagtcgcag
    24961 tgatgtgcgt tcgctatttg gtatggtctt gcaagatgca tggctttatc aaggaacgat
    25021 tgcagataat atccgcttcg gaaagcttga tgcgacagat tatgaagtag ttgatgctgc
    25081 gaaaacagca aacgtagatc attttatccg aacgatgcca gatggatatg atatggccat
    25141 caattctgag ggagacaatg tgtctttagg acaaaaacag ttgctgacga ttgcccgagc
    25201 agtggtctcc gatcctaaaa tcttgatttt agatgaagca acaagttcag tagacacccg
    25261 tttagaagcg ttgattcaaa aagcaatgga tcgcgtgatg gagggacgga caagtttcgt
    25321 catcgctcac cgtttatcga caatccgtga tgcagatctg attctagtga tggatcaagg
    25381 tcagatcatt gaaaaaggaa cgcatgagag tttattggca caaggtggat tttatgaaaa
    25441 actgtataac agccaatttg ctgaggaagc agaataagtc atataagaat aaaacagttt
    25501 aaaacttatt ttttgttctc tattcttgat aatggtaaag taaagttatc aagaaaagtg
    25561 aggacacgga gatggaacag tttttaaatg aaaaaattac tgaagccaaa agagaagttg
    25621 atcgtttaac gcacgtctgt cagacgaagc cttttgattc agctgatttt tttgaaaatg
    25681 agtttcaaat cgaacgcttg gcagcacaaa tagaagtgta cgaattaatt ttaaaaaaag
    25741 taatagaaga aaatatagat gtacaataaa aacaggtaaa acgtcagtta ccagatactg
    25801 taaatagaca tctggcctga gcaggtttta cctgtttttt ttcttatgaa ataaatatat
    25861 gctatgatac ctttattcaa taggattagt ttctacaaaa aatgaaagga gaaaaataaa
    25921 tatggaacgg tttatagcat caaatgccac agtgattggt gatgtgacat tgtcagaaga
    25981 tgtgacgatc tggtatcaag ctgttttacg cggggacagt aattggataa aaataggaca
    26041 aagaacgaat atccaagatg gtacaatcat ccatgtcgat catgatgcac ctgtagatat
    26101 tgcggagaat gtgacggtag gacatcagtg catgctacat ggctgtacaa tcgaaaaagg
    26161 ggcgctgatc gggatgggga ccacgatatt gaatcatgcg gttattggtg agaatagttt
    26221 gattggtgct ggatcattag taacagaagg aaaagttatt cctcctaatg tcttggcttt
    26281 tggtcgccca gctaaagtga tccgtccttt gacaaaagaa gaaatacaaa aaaacaaaga
    26341 aaacatccag cattatgtcg agataggtcg gatgcatgcg caaggaaaat tcaaggagca
    26401 aagataggca gtagcatcca taaaataaaa aaggatggtc agtttgagtt gaccatcctt
    26461 ttttatttgg tttagataag agagcttaaa aaagcttcct ttgaaacaga ttaatttaat
    26521 gaagaactgt tttccatgac ttcatcgatc aatccatatg cttttgcttg ttcagctgtc
    26581 atatagttgt cacgatctgt atctttttca atcacttcaa gcggttgtcc agtgcgttca
    26641 gctaaaattt tgtttaagcg ttcgcgtgtt tgcaaaatat ggcgtgccgc aatttcgatt
    26701 tcagttgctt gcccttgagc gccgccaaga ggttgatgga tcatgatctc agcatttggt
    26761 aaagcaaatc gtttaccttt tgtaccagct gtcagcaaga agcttcccat tgaagcagcc
    26821 atacccataa caattgtttg gacatctgct ttgacaaagt tcattgtgtc gtaaatagcc
    26881 atacctgctg taactgaacc accaggagaa ttgatataaa gatagatatc tttttcagaa
    26941 tcttgagcgt ctaagaaaag caattgggcg atgatcgaat tagcaagatc atctgttact
    27001 tggccgctta acatgatgat acggtctttc aataaacgtg agtagatatc gtaagctctt
    27061 tcacctcgtg aagattgttc aatgactgta ggaattaaat tcataataga gttcctcctt
    27121 gatttcgttt agaaatagtg taccataatt tagtttctaa tacctaaaat gtagtataca
    27181 tttttggtca aaaaaggtca aataaaaaac ttaggcaaat tagtcgcata aacccgcttt
    27241 atggtatgat aagcagagaa acaaaacaag gaggaacaac agattgatag ttggtattac
    27301 tggccttatc ggagtgctga caggtgctgt agttgtctat atcggcatga tactgcaaat
    27361 gcgtcacttg gaaaataaag aacagcgtag acgtgaattg gaattgaaag tcaaagaaat
    27421 cgagacgctg aatcaattaa ataaaaagat caacgagatc ttgcaaaaac ggacaacgct
    27481 gatggaagaa tatgtcagct ttgatgcatt tgatgattgt tatatcacga ttgacgattt
    27541 tgcgtatctc caatcatttt cagcgcaaaa tagtttttat ctgccaaatt atattttaga
    27601 cgagctattt aagaatatct cccatcgaaa agtgatttta tctccagatg aaacaatgaa
    27661 gattggtgga tatgcttata aaggcggacg cgtgatttta gagaatttta cggatagtct
    27721 gactgaaatg atcaatgaaa aaaaagctca gttgaagact atgacgcgcc agccgattgg
    27781 tttcttctcg aaagatgatg tttggagctg atattttgaa gcattgccgt tgcacatgag
    27841 agcgtggtaa tgctttcttt ttttcttaat gatacaatag aactgaacgg agtgaagaaa
    27901 gatgttacaa atctatgccc atcgcggaag cagcggcacc cacccagaaa atacgcttcc
    27961 tgcatttgca gaagctgtac gagtaggtgc agatggtatc gaactagatg tccatctaag
    28021 caaagatggt tatttgatcg ttatgcacga cgaagaagtg gaccggacga ctaacggtaa
    28081 agggctgatt cgagaaaaaa cattggaaga actcaaaaaa ttgaatgcag ggggcaaatt
    28141 cagcaaagaa tttccagcag ctaaagttcc tgaattgaga gaagtgttag gactgctcct
    28201 ccaaaaaaat taccgaggga tgctaatgat tgaaattaaa actgatcagt atgaatatcc
    28261 cggtatcgaa gaaaaattag ccgaaacatt aaaaagtaga gattggcctt ttcaacatgc
    28321 atattgcagc tttcatttgg attctttgga aaaaatcgca ttactggaac cggagataca
    28381 gctggactta ttgatgagta cttctgataa aaagatccaa ctagcagcag aaacccctta
    28441 tatagaagga gtacatccaa aaaatacttg gttgttcgaa catccagtcc aggcagctga
    28501 ttttccaaaa gcaattcgtg catggacgat taacgatgaa gaagaaatgg ccaaatgtca
    28561 agaactcaaa atagatggaa taataactga ttttccggag aaagctcttc ggtggaaaca
    28621 atcaacgaaa agtaggtaaa atatggaaaa agtattagtg atcgtcggtc cgacagcagt
    28681 cggtaaaaca gcgctaagtg tggaactcgc acaactattt ggaggagaga tcatcagtgg
    28741 tgattcattg caaatctata aaaatctgga tatcggtaca gcaaaaatca aagaagaaga
    28801 gatgaataat gttccgcatc atttgattga tgtgatcgaa cctactcaac gttactcagc
    28861 cgctgatttt cagcattcag gtcgacaatt gattaccgag attgcgaacc gagggcacct
    28921 acctatcata gctggtggta cgggtctcta tatccaatcc cttctatatg attatcagtt
    28981 aggagcgaca gaagaagcga atcctgagat tagaaaaaag tatgaaacgt tagctgaaac
    29041 aattggttcc caagcattat ggaatcagtt gaaagaaaaa gacccggcag ctgctgagaa
    29101 aatccactgg aacaatcaac gcaaagtgat aagagctctg gaagttttag agacgacagg
    29161 acatagtatc acggcaccta aagaagagcc aaagcaattg tatgattacc tcatgattgg
    29221 attagatacg aagcgtagta ttctttatga acgaatcaat caaagagtcg atttgatgct
    29281 agcagaaggt ttagtagcag aagcgcgtaa ggtgtatgag cttggagaag tccaggccag
    29341 tcaaggtatt ggatataaag aatttttccc ttattttgaa gggaaagaaa cattggaaga
    29401 agcaacagaa caagtcaaac tacattcgag aagatacgcg aaacgtcagt tgacgtggtt
    29461 tcgtaaccgt ttggatgcac agtggtttga tcttataagt gagccaaatc aaaaaagtca
    29521 gattgagcaa acaatcaaaa aatggctgga ggcggaataa aatggaagaa aaagtgatca
    29581 tcgtaggggt agaaaccgaa gcaaaccagc gttacttttc agagtcaatg gaagagctag
    29641 ttcagctgac gaataccgca tcaggagaag tcgtattcac aattacacaa aagagaccac
    29701 aagtggatcg gcaaacgatt atcggtaaag ggaaattaca agaattagtt caacaagcgg
    29761 atgcgcatga agcggatttg atcatcttta atcatgagct aactccgaga cagagccaac
    29821 tgattaccga tgctgttggt cttccggtaa ttgatcgcgt gcagcttatc ttagatattt
    29881 ttgcgatgcg tgctcgttcg aaagaaggga agctgcaggt tgaacttgct cagttagaat
    29941 acctgctgcc tcgtttagta gggcaaggga aaacattatc tcgtcttggc ggtgggatcg
    30001 gaacaagagg gccaggtgaa acaaagttag aaacagaccg ccgacatata cggaataaaa
    30061 tttttgctgt taagaaagaa ttgaaagaag tagaagctca tcgagagcgc aatcgccaaa
    30121 aaagaaaaaa tagcgagatt tttcagattg gacttattgg ctacacgaat gccgggaagt
    30181 caactatctt gaatctattg acacaagcgg atacttattc gaaagatcaa ttgtttgcaa
    30241 cgttagatcc attgactaag agatggcgtt ttgctgaagg atttgagatt actgtaacgg
    30301 ataccgtcgg gttcatccaa gacctgccta cgcaactgat cgatgctttc cattctacat
    30361 tagaagaaag tcaaaatatg gatcttttgc tacatgtagt cgatgctagt tctccagatc
    30421 gtattttaca ggagcagact gtcttaaaat tgatggatga actgaatatg aaagaaatgc
    30481 cgatactgac tgtttacaat aaggcagacc agatcgaccc agcgatgttc acgcctacgc
    30541 tattcccaaa tgtattgatt tctactcaga cgaaagaagg taagatggcg ttgatcgaag
    30601 caatcaaacg tcagctgatg gaactaatga ccccttatac actttctgtg ccaagcaacg
    30661 aaggacagaa attaagcgag ttgagaagac aaacaatggt tttggacgaa gcgtacatgg
    30721 aagaaacaaa cgaatatgaa gttaaagggt ttgcaaaaac caattcaaaa tggttaagaa
    30781 aaaaccaata aaattttatt tttttaa
//
LOCUS       DADVXO010000019        30577 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.019, whole genome shotgun sequence.
ACCESSION   DADVXO010000019 DADVXO010000000
VERSION     DADVXO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.019"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(51..3725)
                     /gene="rpoC"
                     /locus_tag="KB950_000744"
     CDS             complement(51..3725)
                     /gene="rpoC"
                     /locus_tag="KB950_000744"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBB6856980.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="KB950_000745"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="KB950_000745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBB6856981.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="KB950_000746"
     CDS             8128..9096
                     /locus_tag="KB950_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6856982.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="KB950_000747"
     CDS             9114..10019
                     /locus_tag="KB950_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6856983.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="KB950_000748"
     CDS             10016..10822
                     /locus_tag="KB950_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6856984.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10958..11947
                     /locus_tag="KB950_000749"
     CDS             10958..11947
                     /locus_tag="KB950_000749"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBB6856985.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="KB950_000750"
     CDS             complement(12264..12788)
                     /locus_tag="KB950_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBB6856986.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..14886
                     /gene="scm"
                     /locus_tag="KB950_000751"
     CDS             13075..14886
                     /gene="scm"
                     /locus_tag="KB950_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBB6856987.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15041..16027)
                     /locus_tag="KB950_000752"
     CDS             complement(15041..16027)
                     /locus_tag="KB950_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBB6856988.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16050..16616)
                     /locus_tag="KB950_000753"
     CDS             complement(16050..16616)
                     /locus_tag="KB950_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBB6856989.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16630..17046)
                     /locus_tag="KB950_000754"
     CDS             complement(16630..17046)
                     /locus_tag="KB950_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBB6856990.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17503..19437)
                     /locus_tag="KB950_000755"
     CDS             complement(17503..19437)
                     /locus_tag="KB950_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6856991.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19718..20674
                     /locus_tag="KB950_000756"
     CDS             19718..20674
                     /locus_tag="KB950_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBB6856992.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20764..21867
                     /locus_tag="KB950_000757"
     CDS             20764..21867
                     /locus_tag="KB950_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBB6856993.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21880..22833
                     /gene="menA"
                     /locus_tag="KB950_000758"
     CDS             21880..22833
                     /gene="menA"
                     /locus_tag="KB950_000758"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBB6856994.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23647..24993)
                     /gene="gor"
                     /locus_tag="KB950_000759"
     CDS             complement(23647..24993)
                     /gene="gor"
                     /locus_tag="KB950_000759"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBB6856995.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25127..26380)
                     /locus_tag="KB950_000760"
     CDS             complement(25127..26380)
                     /locus_tag="KB950_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB6856996.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26386..27309)
                     /locus_tag="KB950_000761"
     CDS             complement(26386..27309)
                     /locus_tag="KB950_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB6856997.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27530..30022)
                     /locus_tag="KB950_000762"
     CDS             complement(27530..30022)
                     /locus_tag="KB950_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBB6856998.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(30029..30496)
                     /locus_tag="KB950_000763"
     CDS             complement(30029..30496)
                     /locus_tag="KB950_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBB6856999.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         8837 a         6509 c         5723 g         9508 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    14161 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    14221 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    14281 tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct
    14341 accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc
    14401 acaagtgata gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca
    14461 agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt
    14521 gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac
    14581 agtacagata catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt
    14641 acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca
    14701 agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac
    14761 aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca
    14821 ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa
    14881 aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt
    14941 tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt
    15001 ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa
    15061 tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct
    15121 ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc
    15181 ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt
    15241 tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta
    15301 aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt
    15361 ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat
    15421 ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac
    15481 ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa
    15541 gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac
    15601 tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac
    15661 agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga
    15721 tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg
    15781 ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg
    15841 gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga
    15901 tcgtttcttt gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc
    15961 gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta
    16021 gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt
    16081 tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa
    16141 agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact
    16201 ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg
    16261 acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct
    16321 aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg
    16381 aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag
    16441 aagctgtctc gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct
    16501 agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg
    16561 atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg
    16621 tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat
    16681 cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg
    16741 tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc
    16801 gatcaagtta taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc
    16861 catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc
    16921 tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa
    16981 aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag
    17041 gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag
    17101 gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca
    17161 tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca
    17221 agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt
    17281 ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt
    17341 ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc
    17401 gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc
    17461 tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg
    17521 ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca
    17581 gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa
    17641 ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt
    17701 tgtcgcactg catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc
    17761 aaagaatacc atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg
    17821 catgatcttt gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta
    17881 tgcataactc aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc
    17941 tccgctggca gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac
    18001 attgtattcc catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa
    18061 ccaagtactt ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa
    18121 ccaaactaaa ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga
    18181 agaatgtcct cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata
    18241 ttggaacata taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt
    18301 cattacgata gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca
    18361 tttggcacca atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc
    18421 gcctttgatc gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg
    18481 aacgatctgc ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat
    18541 atagatgttt ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg
    18601 tccgcgtgct gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt
    18661 ccaaatcagt gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc
    18721 cacttctacg attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc
    18781 agcttttgca gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac
    18841 catcagtgtg atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc
    18901 gatcagttcc ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag
    18961 catggcttta cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat
    19021 atgttcccga atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac
    19081 accaggtgta ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg
    19141 ataggaacct aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc
    19201 tgtgacaagt tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc
    19261 ttctggttct acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga
    19321 atgacgatcg atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt
    19381 tgtagctgaa acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga
    19441 tcatccgctc cttatagact atctatataa tgaatatatg ataattcttt tagtttgata
    19501 cttattacac aatctccata tattatatag atgttctgtt cttttgtcta actccttttg
    19561 gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt
    19621 tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa
    19681 tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat
    19741 gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg
    19801 cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca
    19861 atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt
    19921 gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt
    19981 tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa
    20041 cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac
    20101 aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag
    20161 cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca
    20221 acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc
    20281 tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt
    20341 gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga
    20401 caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag
    20461 cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg
    20521 cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca
    20581 ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac
    20641 aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg
    20701 gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa
    20761 aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta
    20821 actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata
    20881 ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg
    20941 tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt
    21001 gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg
    21061 atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat
    21121 agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga
    21181 atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta
    21241 gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa
    21301 ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa
    21361 gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc
    21421 ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac
    21481 atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct
    21541 ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca
    21601 aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca
    21661 attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa
    21721 tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa
    21781 gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg
    21841 atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta
    21901 gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta
    21961 ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga
    22021 atgctagttt ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct
    22081 cattctgaag tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta
    22141 ttgattcgaa acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta
    22201 acgatcaata cgggttggct tttacttttg atgggattta tctgttgttt tattggagta
    22261 ttctatacat tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg
    22321 gtgactatgg gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat
    22381 gtctatgatc tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg
    22441 gctatagcag caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg
    22501 ttggctaata atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt
    22561 ttttatatcg gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat
    22621 ttcgtgattt taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc
    22681 ttgaccttgc caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct
    22741 atcagtttca gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg
    22801 ctattactaa caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg
    22861 ccctgcatca agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt
    22921 tgggcttatt accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag
    22981 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
    23041 aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc
    23101 agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt
    23161 tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta
    23221 actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa
    23281 cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc
    23341 ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt
    23401 ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt
    23461 catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt
    23521 tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca
    23581 gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat
    23641 attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt
    23701 gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc
    23761 atcgacacca ataccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat
    23821 caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg
    23881 tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa
    23941 accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag
    24001 ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc
    24061 aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt
    24121 tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt
    24181 ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc
    24241 agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc
    24301 tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt
    24361 gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac
    24421 ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa
    24481 aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta
    24541 ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg
    24601 agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc
    24661 ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc
    24721 aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg
    24781 gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat
    24841 aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat
    24901 cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc
    24961 gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa
    25021 tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa
    25081 ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc
    25141 ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat
    25201 ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga
    25261 aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct
    25321 gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat
    25381 atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt
    25441 aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct
    25501 tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg
    25561 acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc
    25621 cgttttcaat caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga
    25681 attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat
    25741 cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca
    25801 tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga
    25861 taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa
    25921 cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa
    25981 aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac
    26041 aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac
    26101 tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa
    26161 cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag
    26221 taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg
    26281 ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg
    26341 gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga
    26401 aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat
    26461 ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt
    26521 tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc
    26581 caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag
    26641 atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc
    26701 ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt
    26761 cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc
    26821 atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa
    26881 aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg
    26941 acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt
    27001 ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc
    27061 ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt
    27121 tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc
    27181 ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt
    27241 atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag
    27301 ttcgatcata attttctcct cctacaactt cataatagta gcatagctct aaaaactttc
    27361 caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa
    27421 agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt gtcacaacct
    27481 cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac tatagggaac
    27541 ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt gaagcaccta
    27601 ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc aatcgatctt
    27661 caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga tcaaatccag
    27721 ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct tgttctttca
    27781 atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca tctttactca
    27841 atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg aaagctttct
    27901 ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca tcttggacat
    27961 tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa gtcataatca
    28021 tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga ccatcatcca
    28081 atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca tctaagagaa
    28141 tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca tcatacccta
    28201 cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata aattctgaca
    28261 tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt gctttggcca
    28321 attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag
    28381 gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg acagcctcat
    28441 cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt tcgctttctt
    28501 ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta gcaacatctt
    28561 cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca gaaacttttt
    28621 gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt
    28681 gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt ttgatcacag
    28741 ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat tcatcgatca
    28801 aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa agattgactg
    28861 ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat cttgtacgta
    28921 atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga atgcgagcaa
    28981 aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct agggtcgttg
    29041 ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc gaagcatcaa
    29101 ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg aataagatta
    29161 cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca aattcaccgc
    29221 gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc ttttcttgca
    29281 tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca atggctgttt
    29341 tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg ctaagaatct
    29401 ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg ttttcttttg
    29461 ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc
    29521 gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca gaatcaccag
    29581 ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata cctaagttga
    29641 tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa atatgttctg
    29701 ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca aaaacttgtt
    29761 tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata ccaccataac
    29821 caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt tcacgtaata
    29881 cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt tctgaactaa
    29941 ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact gcttgggctc
    30001 tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt aaacgttcca
    30061 gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt
    30121 tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt tggtcttcat
    30181 aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca aataactgat
    30241 ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt cgtatataac
    30301 caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa cgggtattaa
    30361 tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca atccttcgaa
    30421 tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt aaatccgagg
    30481 tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa ttataacgaa
    30541 tttgataata gaaagaaaga agaacgcttt ttgttaa
//
LOCUS       DADVXO010000020        30112 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.020, whole genome shotgun sequence.
ACCESSION   DADVXO010000020 DADVXO010000000
VERSION     DADVXO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.020"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..616
                     /locus_tag="KB950_000764"
     CDS             <1..616
                     /locus_tag="KB950_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBB6857000.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="KB950_000765"
     CDS             613..1386
                     /locus_tag="KB950_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBB6857001.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="KB950_000766"
     CDS             1383..1781
                     /locus_tag="KB950_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBB6857002.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="KB950_000767"
     CDS             1966..2637
                     /locus_tag="KB950_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857003.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="KB950_000768"
     CDS             2696..3136
                     /locus_tag="KB950_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857004.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="KB950_000769"
     CDS             3148..3816
                     /locus_tag="KB950_000769"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBB6857005.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="KB950_000770"
     CDS             3880..4503
                     /locus_tag="KB950_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBB6857006.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="KB950_000771"
     CDS             4559..4945
                     /locus_tag="KB950_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBB6857007.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="KB950_000772"
     CDS             5158..6549
                     /locus_tag="KB950_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBB6857008.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="KB950_000773"
     CDS             7160..7528
                     /locus_tag="KB950_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBB6857009.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="KB950_000774"
     CDS             7708..8649
                     /locus_tag="KB950_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBB6857010.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="KB950_000775"
     CDS             8718..9449
                     /locus_tag="KB950_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBB6857011.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="KB950_000776"
     CDS             9593..11773
                     /locus_tag="KB950_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBB6857012.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="KB950_000777"
     CDS             11793..12275
                     /locus_tag="KB950_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBB6857013.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="KB950_000778"
     CDS             12291..13052
                     /locus_tag="KB950_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBB6857014.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /locus_tag="KB950_000779"
     CDS             13183..14517
                     /locus_tag="KB950_000779"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBB6857015.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="KB950_000780"
     CDS             14824..15309
                     /locus_tag="KB950_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBB6857016.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="KB950_000781"
     CDS             15302..15985
                     /locus_tag="KB950_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBB6857017.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="KB950_000782"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="KB950_000782"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBB6857018.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="KB950_000783"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="KB950_000783"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBB6857019.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /locus_tag="KB950_000784"
     CDS             19601..20230
                     /locus_tag="KB950_000784"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBB6857020.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="KB950_000785"
     CDS             20282..20614
                     /locus_tag="KB950_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBB6857021.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="KB950_000786"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="KB950_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBB6857022.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="KB950_000787"
     CDS             21440..22813
                     /locus_tag="KB950_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBB6857023.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="KB950_000788"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="KB950_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBB6857024.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="KB950_000789"
     CDS             23868..24308
                     /locus_tag="KB950_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB6857025.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24452..25153
                     /locus_tag="KB950_000790"
     CDS             24452..25153
                     /locus_tag="KB950_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBB6857026.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25150..26271
                     /locus_tag="KB950_000791"
     CDS             25150..26271
                     /locus_tag="KB950_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBB6857027.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            26286..27518
                     /gene="pepT"
                     /locus_tag="KB950_000792"
     CDS             26286..27518
                     /gene="pepT"
                     /locus_tag="KB950_000792"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBB6857028.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27650..28558
                     /locus_tag="KB950_000793"
     CDS             27650..28558
                     /locus_tag="KB950_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBB6857029.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28593..28865
                     /locus_tag="KB950_000794"
     CDS             28593..28865
                     /locus_tag="KB950_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBB6857030.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28876..29922
                     /locus_tag="KB950_000795"
     CDS             28876..29922
                     /locus_tag="KB950_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBB6857031.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT        10026 a         5157 c         6605 g         8324 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa tacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa
    24421 aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt
    24481 agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca
    24541 cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga
    24601 agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga
    24661 ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac
    24721 agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa
    24781 gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg
    24841 tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga
    24901 agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta
    24961 caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa
    25021 atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc
    25081 agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt
    25141 tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct
    25201 cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca
    25261 atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag
    25321 aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg
    25381 gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc
    25441 tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa
    25501 cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa
    25561 ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct
    25621 ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga
    25681 tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt
    25741 caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc
    25801 atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca
    25861 gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca
    25921 tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa
    25981 aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat
    26041 ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg
    26101 gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg
    26161 ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat
    26221 attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga
    26281 atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct
    26341 ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat
    26401 tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat
    26461 tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt
    26521 cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact
    26581 atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag
    26641 attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat
    26701 tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga
    26761 tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa
    26821 tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag
    26881 tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata
    26941 ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc
    27001 tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg
    27061 aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta
    27121 tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca
    27181 cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt
    27241 atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag
    27301 cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa
    27361 cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag
    27421 aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg
    27481 tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg
    27541 acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg
    27601 gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct
    27661 gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt
    27721 tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc
    27781 atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt
    27841 aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc
    27901 tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga
    27961 gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat
    28021 agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc
    28081 agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat
    28141 caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga
    28201 cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg
    28261 cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat
    28321 cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa
    28381 attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca
    28441 ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt
    28501 atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa
    28561 atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt
    28621 ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc
    28681 aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag
    28741 gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga
    28801 agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc
    28861 attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt
    28921 ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc
    28981 tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca
    29041 gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat
    29101 tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg
    29161 tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta
    29221 tcccagggag caggggtgac agcactccat atgattatgt acaaactggc agtgatcact
    29281 tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg
    29341 atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg
    29401 atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa
    29461 agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa
    29521 acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg
    29581 ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa
    29641 atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt
    29701 cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt
    29761 gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg
    29821 ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa
    29881 ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac
    29941 cctttttatc aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc
    30001 tttttttata tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa
    30061 agtcaaaaaa ctatgtgtat attttgacca aaattgacca aagcgatata at
//
LOCUS       DADVXO010000021        29097 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.021, whole genome shotgun sequence.
ACCESSION   DADVXO010000021 DADVXO010000000
VERSION     DADVXO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.021"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1031)
                     /locus_tag="KB950_000796"
     CDS             complement(<1..1031)
                     /locus_tag="KB950_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6857032.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="KB950_000797"
     CDS             complement(1113..1802)
                     /locus_tag="KB950_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6857033.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KB950_000798"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KB950_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBB6857034.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="KB950_000799"
     CDS             complement(2329..2832)
                     /locus_tag="KB950_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBB6857035.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KB950_000800"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KB950_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBB6857036.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KB950_000801"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KB950_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBB6857037.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="KB950_000802"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="KB950_000802"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBB6857038.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="KB950_000803"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="KB950_000803"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBB6857039.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="KB950_000804"
     CDS             complement(6444..7463)
                     /locus_tag="KB950_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBB6857040.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="KB950_000805"
     CDS             complement(7636..8541)
                     /locus_tag="KB950_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6857041.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KB950_000806"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KB950_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBB6857042.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KB950_000807"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KB950_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBB6857043.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KB950_000808"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KB950_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6857044.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="KB950_000809"
     CDS             9754..10398
                     /locus_tag="KB950_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6857045.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="KB950_000810"
     CDS             complement(10454..10957)
                     /locus_tag="KB950_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBB6857046.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="KB950_000811"
     CDS             11554..12519
                     /locus_tag="KB950_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBB6857047.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="KB950_000812"
     CDS             complement(12618..13790)
                     /locus_tag="KB950_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857048.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="KB950_000813"
     CDS             complement(13777..14922)
                     /locus_tag="KB950_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857049.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="KB950_000814"
     CDS             complement(14936..15868)
                     /locus_tag="KB950_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857050.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="KB950_000815"
     CDS             15985..17064
                     /locus_tag="KB950_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6857051.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="KB950_000816"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="KB950_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="KB950_000817"
     CDS             complement(17789..18016)
                     /locus_tag="KB950_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6857052.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="KB950_000818"
     CDS             complement(18119..20707)
                     /locus_tag="KB950_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBB6857053.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="KB950_000819"
     CDS             complement(20727..21386)
                     /locus_tag="KB950_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6857054.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="KB950_000820"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="KB950_000820"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBB6857055.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="KB950_000821"
     CDS             complement(22159..23025)
                     /locus_tag="KB950_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6857056.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="KB950_000822"
     CDS             23134..23775
                     /locus_tag="KB950_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBB6857057.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="KB950_000823"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="KB950_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBB6857058.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="KB950_000824"
     CDS             complement(25216..26115)
                     /locus_tag="KB950_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6857059.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="KB950_000825"
     CDS             complement(26117..26914)
                     /locus_tag="KB950_000825"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBB6857060.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="KB950_000826"
     CDS             complement(26892..27596)
                     /locus_tag="KB950_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBB6857061.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="KB950_000827"
     CDS             27727..28302
                     /locus_tag="KB950_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBB6857062.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="KB950_000828"
     CDS             28420..29097
                     /locus_tag="KB950_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBB6857063.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8560 a         6167 c         5232 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DADVXO010000022        28086 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.023, whole genome shotgun sequence.
ACCESSION   DADVXO010000022 DADVXO010000000
VERSION     DADVXO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.023"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(97..576)
                     /locus_tag="KB950_000829"
     CDS             complement(97..576)
                     /locus_tag="KB950_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6857064.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(649..933)
                     /locus_tag="KB950_000830"
     CDS             complement(649..933)
                     /locus_tag="KB950_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857065.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(1011..1205)
                     /gene="ssrS"
                     /locus_tag="KB950_000831"
     ncRNA           complement(1011..1205)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KB950_000831"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1277..2563)
                     /locus_tag="KB950_000832"
     CDS             complement(1277..2563)
                     /locus_tag="KB950_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBB6857066.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            2701..3372
                     /locus_tag="KB950_000833"
     CDS             2701..3372
                     /locus_tag="KB950_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBB6857067.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3338..3826
                     /locus_tag="KB950_000834"
     CDS             3338..3826
                     /locus_tag="KB950_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBB6857068.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            3839..4786
                     /gene="prmA"
                     /locus_tag="KB950_000835"
     CDS             3839..4786
                     /gene="prmA"
                     /locus_tag="KB950_000835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBB6857069.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            4787..5551
                     /locus_tag="KB950_000836"
     CDS             4787..5551
                     /locus_tag="KB950_000836"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBB6857070.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            5784..7997
                     /locus_tag="KB950_000837"
     CDS             5784..7997
                     /locus_tag="KB950_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBB6857071.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            8008..8454
                     /locus_tag="KB950_000838"
     CDS             8008..8454
                     /locus_tag="KB950_000838"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBB6857072.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            8625..9884
                     /locus_tag="KB950_000839"
     CDS             8625..9884
                     /locus_tag="KB950_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBB6857073.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9973..10920)
                     /locus_tag="KB950_000840"
     CDS             complement(9973..10920)
                     /locus_tag="KB950_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6857074.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            11314..12606
                     /locus_tag="KB950_000841"
     CDS             11314..12606
                     /locus_tag="KB950_000841"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBB6857075.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            12621..14390
                     /gene="aspS"
                     /locus_tag="KB950_000842"
     CDS             12621..14390
                     /gene="aspS"
                     /locus_tag="KB950_000842"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBB6857076.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            14499..15374
                     /locus_tag="KB950_000843"
     CDS             14499..15374
                     /locus_tag="KB950_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6857077.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            15640..17523
                     /locus_tag="KB950_000844"
     CDS             15640..17523
                     /locus_tag="KB950_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBB6857078.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(17598..17882)
                     /locus_tag="KB950_000845"
     CDS             complement(17598..17882)
                     /locus_tag="KB950_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6857079.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(17872..18606)
                     /locus_tag="KB950_000846"
     CDS             complement(17872..18606)
                     /locus_tag="KB950_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBB6857080.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            18735..19358
                     /locus_tag="KB950_000847"
     CDS             18735..19358
                     /locus_tag="KB950_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBB6857081.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            19550..20671
                     /locus_tag="KB950_000848"
     CDS             19550..20671
                     /locus_tag="KB950_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBB6857082.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            20678..23806
                     /locus_tag="KB950_000849"
     CDS             20678..23806
                     /locus_tag="KB950_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBB6857083.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            23825..27019
                     /locus_tag="KB950_000850"
     CDS             23825..27019
                     /locus_tag="KB950_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBB6857084.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(27165..27872)
                     /locus_tag="KB950_000851"
     CDS             complement(27165..27872)
                     /locus_tag="KB950_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6857085.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9357 a         5016 c         5981 g         7732 t
ORIGIN      
        1 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
       61 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
      121 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
      181 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
      241 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
      301 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
      361 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
      421 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
      481 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
      541 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
      601 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
      661 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
      721 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
      781 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
      841 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
      901 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
      961 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
     1021 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
     1081 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
     1141 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
     1201 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
     1261 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
     1321 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
     1381 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
     1441 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
     1501 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
     1561 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
     1621 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
     1681 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
     1741 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
     1801 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
     1861 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
     1921 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
     1981 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
     2041 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
     2101 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
     2161 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
     2221 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
     2281 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
     2341 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
     2401 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
     2461 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
     2521 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
     2581 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
     2641 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
     2701 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
     2761 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
     2821 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
     2881 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
     2941 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
     3001 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
     3061 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
     3121 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
     3181 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
     3241 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
     3301 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
     3361 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
     3421 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
     3481 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
     3541 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
     3601 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
     3661 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
     3721 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
     3781 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
     3841 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
     3901 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
     3961 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
     4021 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
     4081 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
     4141 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
     4201 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
     4261 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
     4321 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
     4381 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
     4441 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
     4501 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
     4561 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
     4621 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
     4681 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
     4741 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
     4801 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
     4861 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
     4921 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
     4981 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
     5041 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
     5101 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
     5161 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
     5221 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
     5281 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
     5341 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
     5401 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
     5461 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
     5521 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
     5581 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
     5641 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
     5701 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
     5761 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
     5821 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
     5881 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
     5941 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
     6001 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
     6061 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
     6121 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
     6181 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
     6241 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
     6301 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
     6361 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
     6421 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
     6481 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
     6541 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
     6601 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
     6661 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
     6721 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
     6781 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
     6841 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
     6901 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
     6961 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
     7021 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
     7081 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
     7141 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
     7201 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
     7261 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
     7321 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
     7381 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
     7441 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
     7501 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
     7561 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
     7621 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
     7681 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
     7741 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
     7801 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
     7861 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
     7921 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
     7981 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
     8041 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
     8101 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
     8161 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
     8221 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
     8281 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
     8341 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
     8401 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
     8461 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
     8521 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
     8581 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
     8641 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
     8701 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
     8761 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
     8821 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
     8881 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
     8941 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
     9001 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
     9061 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
     9121 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
     9181 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
     9241 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
     9301 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
     9361 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
     9421 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
     9481 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
     9541 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
     9601 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
     9661 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
     9721 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
     9781 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
     9841 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
     9901 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
     9961 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    10021 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    10081 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    10141 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    10201 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    10261 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    10321 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    10381 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    10441 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    10501 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    10561 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    10621 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    10681 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    10741 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    10801 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    10861 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    10921 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    10981 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    11041 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    11101 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    11161 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    11221 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    11281 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    11341 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    11401 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    11461 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    11521 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    11581 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    11641 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    11701 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    11761 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    11821 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    11881 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    11941 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    12001 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    12061 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    12121 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    12181 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    12241 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    12301 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    12361 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    12421 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    12481 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    12541 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    12601 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    12661 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    12721 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    12781 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    12841 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    12901 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    12961 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    13021 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    13081 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    13141 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    13201 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    13261 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    13321 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    13381 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    13441 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    13501 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    13561 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    13621 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    13681 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    13741 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    13801 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    13861 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    13921 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    13981 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    14041 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    14101 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    14161 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    14221 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    14281 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    14341 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    14401 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    14461 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    14521 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    14581 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    14641 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    14701 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    14761 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    14821 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    14881 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    14941 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    15001 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    15061 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    15121 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    15181 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    15241 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    15301 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    15361 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    15421 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    15481 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    15541 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    15601 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    15661 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    15721 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    15781 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    15841 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    15901 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    15961 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    16021 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    16081 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    16141 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    16201 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    16261 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    16321 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    16381 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    16441 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    16501 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    16561 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    16621 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    16681 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    16741 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    16801 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    16861 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    16921 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    16981 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    17041 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    17101 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    17161 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    17221 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    17281 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    17341 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    17401 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    17461 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    17521 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    17581 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    17641 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    17701 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    17761 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    17821 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    17881 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    17941 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    18001 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    18061 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    18121 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    18181 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    18241 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    18301 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    18361 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    18421 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    18481 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    18541 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    18601 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    18661 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    18721 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    18781 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    18841 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    18901 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    18961 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    19021 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    19081 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    19141 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    19201 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    19261 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    19321 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    19381 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    19441 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    19501 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    19561 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    19621 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    19681 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    19741 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    19801 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    19861 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    19921 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    19981 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    20041 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    20101 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    20161 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    20221 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    20281 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    20341 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    20401 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    20461 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    20521 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    20581 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    20641 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    20701 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    20761 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    20821 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    20881 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    20941 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    21001 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    21061 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    21121 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    21181 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    21241 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    21301 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    21361 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    21421 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    21481 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    21541 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    21601 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    21661 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    21721 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    21781 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    21841 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    21901 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    21961 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    22021 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    22081 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    22141 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    22201 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    22261 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    22321 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    22381 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    22441 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    22501 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    22561 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    22621 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    22681 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    22741 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    22801 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    22861 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    22921 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    22981 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    23041 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    23101 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    23161 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    23221 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    23281 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    23341 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    23401 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    23461 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    23521 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    23581 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    23641 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    23701 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    23761 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    23821 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    23881 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    23941 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    24001 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    24061 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    24121 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    24181 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    24241 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    24301 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    24361 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    24421 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    24481 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    24541 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    24601 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    24661 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    24721 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    24781 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    24841 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    24901 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    24961 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    25021 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    25081 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    25141 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    25201 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    25261 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    25321 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    25381 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    25441 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    25501 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    25561 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    25621 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    25681 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    25741 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    25801 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    25861 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    25921 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    25981 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    26041 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    26101 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    26161 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    26221 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    26281 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    26341 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    26401 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    26461 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    26521 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    26581 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    26641 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    26701 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    26761 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    26821 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    26881 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    26941 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    27001 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    27061 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    27121 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    27181 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    27241 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    27301 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    27361 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    27421 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    27481 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    27541 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    27601 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    27661 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    27721 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    27781 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    27841 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    27901 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    27961 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    28021 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    28081 tagaag
//
LOCUS       DADVXO010000023        27386 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.024, whole genome shotgun sequence.
ACCESSION   DADVXO010000023 DADVXO010000000
VERSION     DADVXO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.024"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(82..648)
                     /locus_tag="KB950_000852"
     CDS             complement(82..648)
                     /locus_tag="KB950_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6857086.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(676..2178)
                     /gene="eat(A)"
                     /locus_tag="KB950_000853"
     CDS             complement(676..2178)
                     /gene="eat(A)"
                     /locus_tag="KB950_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBB6857087.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2436..3557)
                     /locus_tag="KB950_000854"
     CDS             complement(2436..3557)
                     /locus_tag="KB950_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6857088.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3683..4624
                     /locus_tag="KB950_000855"
     CDS             3683..4624
                     /locus_tag="KB950_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBB6857089.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4774..5670)
                     /locus_tag="KB950_000856"
     CDS             complement(4774..5670)
                     /locus_tag="KB950_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBB6857090.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5939..7279
                     /locus_tag="KB950_000857"
     CDS             5939..7279
                     /locus_tag="KB950_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6857091.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7497..7940
                     /locus_tag="KB950_000858"
     CDS             7497..7940
                     /locus_tag="KB950_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBB6857092.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8444..9862)
                     /gene="nhaC"
                     /locus_tag="KB950_000859"
     CDS             complement(8444..9862)
                     /gene="nhaC"
                     /locus_tag="KB950_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBB6857093.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10265..11488)
                     /locus_tag="KB950_000860"
     CDS             complement(10265..11488)
                     /locus_tag="KB950_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBB6857094.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11519..12358)
                     /locus_tag="KB950_000861"
     CDS             complement(11519..12358)
                     /locus_tag="KB950_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBB6857095.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12375..13055)
                     /locus_tag="KB950_000862"
     CDS             complement(12375..13055)
                     /locus_tag="KB950_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857096.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13057..14130)
                     /locus_tag="KB950_000863"
     CDS             complement(13057..14130)
                     /locus_tag="KB950_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6857097.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14186..14424)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14467..14700)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14777..15448)
                     /locus_tag="KB950_000864"
     CDS             complement(14777..15448)
                     /locus_tag="KB950_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6857098.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15535..18213)
                     /gene="mgtA"
                     /locus_tag="KB950_000865"
     CDS             complement(15535..18213)
                     /gene="mgtA"
                     /locus_tag="KB950_000865"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBB6857099.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18872..19309
                     /gene="ndk"
                     /locus_tag="KB950_000866"
     CDS             18872..19309
                     /gene="ndk"
                     /locus_tag="KB950_000866"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBB6857100.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19331..20197)
                     /locus_tag="KB950_000867"
     CDS             complement(19331..20197)
                     /locus_tag="KB950_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6857101.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20357..22450)
                     /locus_tag="KB950_000868"
     CDS             complement(20357..22450)
                     /locus_tag="KB950_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6857102.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23199..23822)
                     /locus_tag="KB950_000869"
     CDS             complement(23199..23822)
                     /locus_tag="KB950_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857103.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23981..25378)
                     /locus_tag="KB950_000870"
     CDS             complement(23981..25378)
                     /locus_tag="KB950_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6857104.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25410..26381)
                     /locus_tag="KB950_000871"
     CDS             complement(25410..26381)
                     /locus_tag="KB950_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6857105.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26755..27180
                     /locus_tag="KB950_000872"
     CDS             26755..27180
                     /locus_tag="KB950_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857106.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         7851 a         5685 c         4781 g         9069 t
ORIGIN      
        1 gaattgtcat aacctaaaaa aggcactgct aagcaaaagc agtgccttga aatattttat
       61 ttaaataaca caaaaacata attactcata agaaaccaat tctgtattat ctaccatgaa
      121 tgtaaagaac ccctcattag gcatcggatg aaaataagca ttgatcactc ttatcaaggc
      181 attatggcaa actaacagat agacattttc ttcgtcttcg atgcattctt tcaataaagg
      241 aacgatccgt gcatacacat ctaagactga ctccccattt gggaatcgta cagcgaacgc
      301 taaacgtgct ttttgaaagt tctcatcttt gcttggcagt ccatcataat ccccaaaatc
      361 catttctacc aatcgttcat caacggtcag cggcaagctg agacgatcag caactgcttg
      421 tgctgtatct cttgctcgtt gaagagggga atggatgatt ttcgtgattg gaacaggcaa
      481 atctactacc ttttctgcta gtttctgtgc ctgcatctgt cctttttcag tcagcggcac
      541 atctgcccgt ccgcaaacta atccttgtgc gttccaagtt gtttctccgt gtctcgttac
      601 ataaagcatg tgattctctc cttcgttggc aaagttcccc ttaaccatac cttgttgttc
      661 ttgagattac aagggttaga ttctctcaag agaaataatc tcagtagata ctttatccag
      721 gaaggtttga tcatgttcca ctagtaacat ggcaggtttc acgttcaaga tcagttgttc
      781 taattgttct tgattgaaga catccaaata attcaatggt tcatcccatg tatatagttc
      841 agctggctgt gataaagatt tagccaattc cacttttttc cgttggccca tactcatctg
      901 ctcgatcttg ttatgaaaaa catctctttc catcccaagc tttcggaggt tgttcaaaaa
      961 tgcttggtag tctacttggt ttttctctgc aaattccgcc aacgttcctc gattgtcttc
     1021 ataattttgg cttgcataac taatgctcag atgttttggc tggtattttt ctcctataac
     1081 cttgccgttg aatgccccca gaagataatg gataatggat gactttcctg caccgttagg
     1141 acctgaaatc gccacccgct gatgaggctc gattgtaaaa tgaattggct cgaataacag
     1201 attttcatac cctaattgaa gatcttctac gcttaaaagt cgcttatggt gagacgcttg
     1261 gctattcatc gtcaaagaat cgatatattc gatatctttt aatagttttt ctttctcgct
     1321 gatctgggtc tccatccgat tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc
     1381 agcacccact gctcctttat tcactcgtct agattcagta tcgatgaatc cgacttgttt
     1441 ctttgtttta tctccttctc gggaacgaga ccattcggct ttttcagctg ctgttttctt
     1501 tagcctactg acttcttttt tcaatttttc attttgagcc atttcaaatt catcacgaag
     1561 ttttttctgt tcttcataga tagagaaatt cccttgataa agttccagtt gacttttttc
     1621 gattgctaaa acatggtcca ctacttcatc gataaatccc cggtcatggc tgaccacgat
     1681 gaagccttgt tttttctttt tcaaataagc cgctacttgt tttctaccag agatatccaa
     1741 atgattcgtt ggttcatcga ttaacgggaa atgagtgtca tccacaaata aaagtgccag
     1801 taggactttt gtcttctctc cccccgatag tgtgctgaat tctctccata agatttcagg
     1861 atctgtttgc atcaattgga gctctctttc gatttcccat atctcaaaat ccgtaatatc
     1921 atttaacacg taataggtta aacgttcttt atcttttgtc tgttgcggga aataggcaaa
     1981 ttcttgctga tggattactt gcccttgata aggtagtttg ttttgtagaa tattcagtaa
     2041 agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt
     2101 tagatttgct tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg tcagattttt
     2161 tatttcgatt ttagacattt tccatcacct cgtaaaaaaa gaccagtcct attttcagaa
     2221 ctggctaaag aggatgcaag ggttggatac acacaaaaag acccatacat cgaaaaaacc
     2281 aagccaatcc cgaaaatctg cacactgctt ccttgtctcc acaaggtaca gtctgaatgc
     2341 agctttcatc aatcgactta atttttcaat gcacggatcc ctcactttgt cctatatttg
     2401 aaattacgat agcataaaga aaatgatttt gtcaactact tcgggaggat ggtgaaaaat
     2461 ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg ttccattgct
     2521 tcttctaaac ctattggacc caaatgaata cttaagacaa ttgggagaaa atcagaaagt
     2581 ccttctagct cttggtctct acttccgcaa atagcgatta ttggttttcg atattttttt
     2641 gcaagcactg ctacaccgta tggaactttt ccgtggatcg tttgctgatc gatccgccct
     2701 tctcctgtaa tgatgacatc tgcttgttcg atcttttttt ccaatccagt gatttggctg
     2761 actaaatcga aaccagaaac catcttcgct cccaacaaaa aaagggctcc accaataccg
     2821 cctgctgctc cacttcctgg aatatcatct aaatccacct cccagaaatc ttttatttgc
     2881 tgagcaatct ttcctgcttg tttatctaag aaagctagcg tcgtatctat tcctcctttt
     2941 tgtcttccaa attgccaagc agctccttgt tgacctgtat atggattggt aacatctgca
     3001 gcgataatca attttacttt agaaacaaga acgtttcttc gatcaaaatg aagtttttta
     3061 gtagataata aaggatttcc ttctgtgtac ccttctaaag aagcacccaa ggattggagt
     3121 aatcctagac caccatcgct acacccgctt cctcctaaag tgacaataat ttgttctact
     3181 tcttgaagca gtgcatcttt aataagttct cctaaggcat aactggaagc aagtcggata
     3241 gtttgatccg ttggtttatc aagtagatct aaccccaatg cttgtgcaga ttcgatgact
     3301 gcggtgtttt ttccttgcca agttgtcaat aaatagctga tttttcgctg acggaataag
     3361 agatccatcc catcaatggt acgccatgtt cccctgagcg cgtaatagat tgcttctgca
     3421 gtattttctc cgccatctgc aatcggcaca ttgatttttc ttcctttacg atcttcccag
     3481 ccatttagaa ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt
     3541 gccgccaaaa tatccatctg tatccctcac tttcttctta agtaagacac tttttttcat
     3601 ttttttcaaa caaaacaatg ctattttctt ccatttttct atatactaaa agggaactca
     3661 ttgaataaag gagagaaaaa acatgaaaat cgcaatagct ggggccggag caatgggcag
     3721 ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc
     3781 tgctcacatt gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt
     3841 ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga
     3901 cttgatcgta gcattaacaa aagccaatca attagatgat atgttttgtt cgatccaatc
     3961 cattattact gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt
     4021 actggaaaaa tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg
     4081 tctagctggt cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt
     4141 ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt
     4201 aaatccgatc tatagccata acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg
     4261 aacattgaat ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa
     4321 agcggcagag agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga
     4381 aggaatcata ctagatcaag aagaagtgta ccaacatatt gcttctactt atgatcctga
     4441 caacatcggg ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac
     4501 tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga caaaaatatg atattgctac
     4561 accatattgt gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga
     4621 ataaaaggct ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc
     4681 aataaaagat caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt
     4741 ttcagtacat aatctcttca aaatgtaggt tttttatttg ttttcaacag aggagaggtc
     4801 atattcgtat tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc
     4861 tgcatcataa aattccaata gctctgatag gattggctta aatggtgtat gcagaaattc
     4921 cggtaattca tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg
     4981 ataatgagca atcgcttgat tatccgtcac tagtttcttt tgaaaataca tggattcaaa
     5041 tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc
     5101 ccctgttttt ttcaggtact cttcataagt aagatatcca ggttgttcat tccctcgatg
     5161 tggaagaatg aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc
     5221 tgctcgttct tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct
     5281 agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt
     5341 tgcttccaaa aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt
     5401 cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt
     5461 ggtaaatggg cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat
     5521 cagctgataa tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc
     5581 ttttccggac caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt
     5641 tgcaactagc agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt
     5701 tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat atttgtatga
     5761 aaatttaaca tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct
     5821 aaaagttttc tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat
     5881 attgctattt attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat
     5941 ggaagataaa aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg
     6001 tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc
     6061 ttctgtattg attgcttttt tgattggtgg gctgatttta tatatcgtga tgaaaggtct
     6121 gggaaaaatc gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc
     6181 ttatctaggt gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat
     6241 caatatcatc gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat
     6301 tccagcctgg gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc
     6361 ggtacggtta ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat
     6421 cttactgatc atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt
     6481 ccctaccctt cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat
     6541 catcagttct ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac
     6601 ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg
     6661 aagaatcatt tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa
     6721 tactttagca ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc
     6781 atttgcagcg gatatcgtca attttgtgat tgtccttgct ttgttctcat ctatcaattc
     6841 gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc
     6901 tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac
     6961 cagtgttctt tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta
     7021 tctggcaggt tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc
     7081 ctttgtactg gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt
     7141 agctgtctta ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt
     7201 attgactggc ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt
     7261 cgtattgacg aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg
     7321 agctattgaa gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac
     7381 agtctcatga cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt
     7441 tctccatgct catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga
     7501 aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga
     7561 tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg
     7621 cgttgatgat gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg
     7681 aaaacaaaaa gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct
     7741 tgtttatggt acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc
     7801 ctcatcattc tatcgctatc ctggcaagta aacgagctga tttaaaagat cctgaagtaa
     7861 aaaaattagc ggaagaaatc attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa
     7921 tgatcaaaga aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag
     7981 ttatcagctt caagaaataa gaagaagcta cttctggaac gccgtgaata aggggttgtg
     8041 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt
     8101 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt
     8161 gtaaaatcag cgttctgacc tgagtattag agagacaaat ggaaaaatag ttcctcatat
     8221 tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt atcgagaggt
     8281 tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat
     8341 ttttgtataa atttggctta ataccgagaa actggctttt gaataccgtt tattcgtaaa
     8401 agggattgtg acaaaagtct tgtcacaatc cctttttatg tttttattgt tctaattgcg
     8461 ctgtcttgct tactgcttct tgcggtttca caaatttttt tgagattccc caacctgtga
     8521 aggcactgac catcgaaaac acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag
     8581 tgagtgtagg aataccaaat gtacttgcta cgaaacttcc ggcaattccc caaggcacta
     8641 agtagttgat tactgttcca ccatcttcta gcgttctacc taatacagtt ggatcaaatc
     8701 ctgctttttc ataagtttct ttaaatgcat taccaggcag aataacagat aggaattgtt
     8761 caccaataaa aatatttact ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg
     8821 tgttcgtcat ctttcttgat actcgttcca tcacggttga gatcaagcca aattcgatca
     8881 acaatccacc taatgacaaa gttagaataa tcaatgacac agtaggcatc atactttcga
     8941 ttcctccgcg gctaagcagt gaatcgattt gttggtttcc agtatttgaa acaaaccctg
     9001 attcgatgac cccagccaac ttcgttaagg aagtttgtgg gttttgaata aaaatcattc
     9061 ctactgctac agcgatattc aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac
     9121 acagtaacat taaaaataaa ggaatcagtg accagattga aatagtaaag tttgcttcta
     9181 gaacatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa
     9241 tcatgaataa aaagaaagaa acaacaaatg ccgggacagt tgaccacatc atatgcttga
     9301 tgtgtttgaa caaatcggca tcaacaatag ctgctgctag atttgttgat tcggataatg
     9361 gagacatctt atctccaaaa acggcacctg aaacaatagc tccaacaacc agcgctggat
     9421 ttaagcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa
     9481 aggcactccc tacagcgcta cctacgattg cacacacaac aaatacagaa gggacaaacc
     9541 actggacact gatcaatttg aacccaatga ccattagagt tggaatgatc cctgcttgta
     9601 tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattgggatg attcctggtt
     9661 tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat
     9721 acaacatgac cattccgatc gctgctaaga tcgtgatatt aggtgagacc tttaatccga
     9781 caacgccaat cgcaatgcct aaaacaataa ccattaatac aagtgttgct tctaaaaaac
     9841 ttggtgttcc tttttttccc atgataatct ctcctttttt agttgttctt atgttctaaa
     9901 aaagttaaga tatcggatta ctccgccgca attcatccgc gattcctcct ttataaaaat
     9961 aaaaagacct gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca
    10021 gatctgtttt tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca
    10081 attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac
    10141 tgcgtgaaaa gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa
    10201 gaaaatattt tttcattttt ctgaaaatta tttttataac tttcacacac aagtttattt
    10261 tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct
    10321 tgattctttc taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt
    10381 ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt
    10441 ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga
    10501 cttggctgac atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt
    10561 cttcatctgg aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag
    10621 taacgatgac atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac
    10681 ttatcgcagc acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc
    10741 gatttcctgc acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta
    10801 ctcctccgtc tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag
    10861 cgttgattcc attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg
    10921 ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag
    10981 attctgtcgt tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct
    11041 caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc
    11101 ctggggtgcc atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc
    11161 tggcggctaa tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg
    11221 gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac
    11281 gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt
    11341 ctaattgttt aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt
    11401 cgtggatatc tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca
    11461 attggtttgt tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt
    11521 attttctccc gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta
    11581 ctttgactgt gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt
    11641 catcttcttt tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt
    11701 ctaagaagat ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga
    11761 tcactgaaat gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa
    11821 tgtctagatt ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct
    11881 tatccacttt gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa
    11941 catcattagg gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt
    12001 tagaataaat tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt
    12061 gttctttatt gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc
    12121 catctgctaa agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa
    12181 tgttctcctt tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa
    12241 tccctagttt tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc
    12301 ctgccaacca aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt
    12361 tttctcctcc actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt
    12421 aaaaaaacta agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga
    12481 ttataccctc tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg
    12541 gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa
    12601 ccttctaaca aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc
    12661 gcttcgatca ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac
    12721 ggaacaactc caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact
    12781 aagaaacgag tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg
    12841 atattgatca gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg
    12901 cgtgtcacta ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc
    12961 caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa
    13021 acattgggca aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt
    13081 gatacgacta tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa
    13141 ataatccaat gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc
    13201 taacggcgtt tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata
    13261 tttgctgtac agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc
    13321 caccagccat ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa
    13381 tgcttggtca agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt
    13441 gctgaagatc tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac
    13501 cttgttgcag atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc
    13561 taactggcga ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag
    13621 tgcactggtt gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat
    13681 tgctactcgt tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc
    13741 caaaccgaca agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc
    13801 agaatacttt aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa
    13861 atgctgaaag atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg
    13921 actcagattc gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat
    13981 cacacgaact aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc
    14041 tttttcaatc gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa
    14101 acatacagaa acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc
    14161 ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg
    14221 ttcaagctgt gttcacacac aactcctctt caagtacgat catactctag caagatatcg
    14281 gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc
    14341 aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg
    14401 tacttaccat ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta
    14461 tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa
    14521 gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt
    14581 gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc
    14641 tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga
    14701 tattttgttg ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa
    14761 attatttttt ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa
    14821 gatcttttcc ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga
    14881 caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc
    14941 ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag
    15001 ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg
    15061 tgtccaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag
    15121 tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg
    15181 catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct
    15241 tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac
    15301 tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa
    15361 attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac
    15421 catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca
    15481 tagtttagca cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa
    15541 gagttcttgg aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc
    15601 gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc
    15661 agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac
    15721 aaacatggca aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg
    15781 gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat
    15841 gaaaatcact cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca
    15901 aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa
    15961 gtaaaccatg aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc
    16021 tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa
    16081 caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga
    16141 aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa
    16201 agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat
    16261 cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc
    16321 cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt
    16381 tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc
    16441 atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt
    16501 aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt
    16561 gtccccagtc agaactttta cagctacacc atgttctttc agagctttca acgcttgttt
    16621 cgttgtttct tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc
    16681 atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac
    16741 tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt
    16801 tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc
    16861 aatcgcgcct tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat
    16921 acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt
    16981 atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt
    17041 ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca
    17101 atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca
    17161 taacacatcg attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt
    17221 tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc
    17281 aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt
    17341 tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag
    17401 catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt
    17461 ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact
    17521 tccactgatg acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg
    17581 atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc
    17641 ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc
    17701 tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt
    17761 tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg
    17821 attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat
    17881 aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa
    17941 tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat
    18001 ttctacaatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct
    18061 tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa
    18121 ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc
    18181 catctcaatc tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc
    18241 tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc
    18301 tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct
    18361 ttactaagta attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt
    18421 agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc
    18481 caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt
    18541 tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa
    18601 gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag
    18661 cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata
    18721 acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata
    18781 cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata
    18841 atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc
    18901 agacggcgtg agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact
    18961 ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca
    19021 gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt
    19081 cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc
    19141 aacaaaagct gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac
    19201 tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt
    19261 tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta
    19321 aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc
    19381 gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct
    19441 tttcttcccc taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat
    19501 agatcactgt acttctttct ttatgtgata agctccgttt catatcttca ttcacactac
    19561 gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa
    19621 agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca
    19681 aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata
    19741 gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat
    19801 aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca
    19861 aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt
    19921 tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact
    19981 ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc
    20041 gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc
    20101 cgactagaga agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt
    20161 tgactcgaag tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa
    20221 atttttccac attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa
    20281 aaatgttttt ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga
    20341 aggttggttt gattgtttat tgattatttt tttgctgttc aacgtctgtt cttgttactg
    20401 gatataagtt ggttgttgtt ttattaccat tttttgaaaa gacacttgtc gaatgttcgc
    20461 ctttttgctt ttcgattgct tcattacgtt ccgcttcatt tttatagtca tattttttcg
    20521 gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa gagattttcc tgattcagct
    20581 tatctgaaag acttaacgca gtcttgactt tttcctgatc tttggagatt tcctcatcga
    20641 ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt attgtaagct tttcctccta
    20701 ggacggtgta tttgggtgtc acaaagttcc catttcgaaa agcaactact tgatcatgct
    20761 gagaagataa taagtctgta ccaaaatgaa gataatcttt atcatcgatt cccaatagat
    20821 gtagtaatgt cggcaaaaca tcgatttctc caccgtattc atgattgact gtccctttag
    20881 tataaccagg catatggatc atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat
    20941 cccatgtatc tggatcttta ccaagagcgc ttgctaaatc tttattatct gtattcgaga
    21001 tcccaaaatg atccccgtaa atcacaaaga ttgaattttg gtaaatacca gattttttca
    21061 aatacgtaaa aaattgttcc aatgattgat ccagataatg tgccgttcga acataattat
    21121 ctactgtgct attgccagta ttcaaagatg gaaagtcgaa attcttgtca tccgtataat
    21181 aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa
    21241 gatattgggc agattctctg aataaatctt tatctaatat cccataacct aacgtttcgt
    21301 cggattcatc aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat
    21361 gatctctatt ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct
    21421 tttgtccaag gatcgctggt gcggcttgaa agacattgtc tgacccaagt tctgtgaata
    21481 atgagccttg aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct
    21541 ttccctgtcc tacttcatga aagaaattgt caaagctgat cgttgcttga ttctggaaaa
    21601 tactatttaa aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct
    21661 ggaaactttc taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg
    21721 gatttggctc tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc
    21781 ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat tccgtcataa atagtatacg
    21841 catcgattcc tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg
    21901 gacggttggc ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtaa
    21961 cagcgatagc catcggtttg ccgacagggt tgcttttgat aggtattttc ttgaccttca
    22021 accaaaccag tagcccaatg aacacagcga tatccagcca gtagatgaca tcatggggct
    22081 tcatcaaaga aaaggagctt cctgacaatc cttgcgacac tttcgaaaag cctaatacag
    22141 atttgactgt aatgaaatct gtgaattccc gataaaaaat aatattcaag tacaacaaaa
    22201 gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc tggctttggc tttttaatat
    22261 acaaagaaag gccaaaaaac aataaggtag tcgcaattgg gttgatccac aagataaagt
    22321 actggatcgt accttccaca cctaatgaaa aatctgcata gtaggcaatg atcgtcttca
    22381 gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa
    22441 gttgtgtcac gtatatcact tctctctatt cacatttttt cttattttgc tcctgaaaaa
    22501 tgaaaatttc ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc
    22561 cgtccacttt tcacagcata aaaccgctgc gatgtaagcg attaaactaa tgaagagata
    22621 aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa gccagtttct cggtattcgt
    22681 acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc ttatttcttg atgcaggaca
    22741 gctttttcac aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca
    22801 caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa
    22861 acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccctc ggtattagat
    22921 tatgtcagga cgctaatttt acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc
    22981 ggtaataagc ccaaccaaac gaaaaatata aggagctatt ttttgaaatt gctccttgtg
    23041 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgacct
    23101 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt gtttctctaa
    23161 tactcaggtc aaaacgctga tttcacaacc tctgtttttc attgtccata ggattgatat
    23221 tgtccttgtg atgactcatt cgtaataatt gaggcttgat tttcttcact tcccgttatc
    23281 gtttgtccac tgccagaatc tgaaggactt gttcctgtcg actcgttttt ttgagaagag
    23341 acagttgttt tcttgacttg ttcatttgtc tttttccatt ccgctaaacg atcggcgacc
    23401 acttttttgt ctactttttc agaataagcg gtgatcactt ttccattttt caaacgggct
    23461 actttgtcgc catcatgagt caaaagcaaa acggtcaaat gcttgtcttt aaattgatat
    23521 tgtctgagag aaatcgcact aagtacgtca tcgatattaa taaattgatt ttcttctttt
    23581 ccttttggca aataaatgtc caattcgcct ttttcgtaat tggcagttat tttatactcc
    23641 gcccaccaat aagttgagaa ttcttttttg aaattatcta ctgattcttt gcttgctacg
    23701 attttttctg tttctttctg agcaagatct gctgtcattt ttcctgaaaa tgctgcggat
    23761 aaaataagca gtaagaaaaa accgaataac caaaagagaa gaaacggtct ttttttcctc
    23821 atgctttcct ccactcctat ttaattttga ctattctatt atatacaaaa aatgaaatag
    23881 aaagacaaaa aagttgaaaa agatcgtaaa taattgaact tctttttcac aaaatggtaa
    23941 gaatggagaa tttcacgcat ttgctctcaa ctattctttc ttaagcctca aatccgtttt
    24001 tatctgatac gtctttgaac caatgacccg attttttgat tgttcttttt ccatcttgtg
    24061 ctaagtctac tgaaataaag ccatatcgat tcttatatgc attggtccac gaccagttat
    24121 ccatacatgt ccacatatga tagcctaaac aattggcacc ttcttgaatg gcttgatgga
    24181 catatttcag atgagattgg acaaattcga tccgataatc atcgtggatc atgccatctt
    24241 ctttgataaa tcgtgcttcg ttttctacac ccataccgtt ttcagaaata aagcagtcga
    24301 tatttccata atgatctcgt acattgatca aaatatcata gattccttgt tcatagatct
    24361 cccatccgcg ataaggattc attttacgac caggcatttc ataattgtcg aaataatcct
    24421 caggcattgg accgttttta gtatcgattg gtgtttcttt tgcttttacc cggcgtggct
    24481 ggtaataatt gatacctaaa agatctactg tattttcttt gataaccgtc aaatcctctg
    24541 catggatcac aggcatgaga tcaagttctt ttactatctc aatcaattca cttggaaact
    24601 cgccttttac agctggatca aggaatgaac gattgaagaa cgcatctgct atttgcgctg
    24661 ctttaacatc ttcagggttt gattcgtctc ttggatagct tggtgtcaga ttcaagatga
    24721 taccaataac accaccttgc tccatttcat ggtaagattt gatggctaac gctgaagcca
    24781 acgcttcatg atagccaact tggatcgctt ttttcatatc tgactcttct ggataatgcc
    24841 atcgatataa ataacctgct tcgactggaa cgattggttc attatgagta aaccattttt
    24901 ttactttcga accaaacaat tcaaaacaac ttttggcata tttttcatag gcttcaaccg
    24961 tttctcggtt caaccatccg cctttttctt gcagatacat cggcatatcg aaatgataca
    25021 gattcacaaa gggttcgata ccgttttcaa tcaactcatc aaaataacga tgatagaaac
    25081 gaacggcttc ttcgttgatt gttccagatt catcaggtat caaacggctc cattggatag
    25141 atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg tacatcctct tgatacttcg
    25201 tataaacttg cgaggttttt tcaggtccta cttgatcaaa gaacttttgc ggttcttttt
    25261 tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt ccttctgttt
    25321 gtgggccact tgctgcagaa ccccaccaaa aatttgctgg aaattgatat tttttcatca
    25381 aagcacctct ttttcttttt tagttcgttt tagttgattc catgtatgat attttccatc
    25441 acaatcactt tttcattttc ttcctcttct tttttgattc gttttaacag ctgttctgct
    25501 gctcgttccc cgattttatc tgttggtggc gtgatcgtga ttttcggaag gatactcaga
    25561 tcgtttttat ctaaataatc atagtgactg aacgcaatat ccttcccgat tctgacacct
    25621 tcttgattga aacattcgat ggcaccaatc gacatattgt aattgctgac aaaaatagca
    25681 gttgcccctt tttctaataa tgcttttgcc ccgtaatagc cgctttttct agaatagtct
    25741 cctgtaaaga cctctaattg gatcgatgga tcattttgga ccgcttcttt ggctcctgct
    25801 aaacgttctc ttgctacgta gtctgtttga gtggcagcaa taaatccgat ttttttatgt
    25861 ccttgttcac catgatgagc gatcactttt gctacacttt ctcgatcatt cactaatatt
    25921 gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga taactggaat actcattttt
    25981 gtgagcattt caaatccaga ccactcttct tcggataaga agacaatcag cccatcgatc
    26041 acatgcttca tcaggtattc aattttttgt tcaatttttt ctggctgatc ggcaaacccg
    26101 cttaataata agctataccc attttcttca gctactcttt cgatccctga gacaacttcc
    26161 gacccaaaac ggctggaaat cccgtttatc aaaagtccaa tagagaagct tcgattattc
    26221 ttcaaccctt tagcaatgat gttttcttta tagtccaacg ctgaaatcgc ttctgctatt
    26281 tttttcatgt tcttttcctt cagttgctga ccattcaagt aacgggaaac tgttcccact
    26341 gaaacaccag ccatcttagc cacatcacgg atcgatgcca tacattctca ctcctgccag
    26401 ttacatttga tttctctttc ttgttgttcg attatgatga ccgcattctt tgcatagtgt
    26461 tgcttgtcat caatatccat ataactaaag tcttcagtag agcaacttcc tctcactatt
    26521 aattatactt aaacaagtat ttgaaccggt tcatttttaa tatagcattt ttttaaaaga
    26581 ttgcaacact tttttaaaac gctttctgaa agtggcggat tttttgcctt tttaaaattt
    26641 tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc atccgtttga
    26701 agttttacat tccaatggta taatttcttt atcctatcaa atgggggaag tattttgaag
    26761 aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg ctgtgatttg
    26821 tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa
    26881 aaagcagata ccaccaacag tgtatatacg ctaaaagtac gtaaagatat ggatttatcc
    26941 gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta tcagacacaa
    27001 ggaactgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat caagatcact
    27061 accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaaac aacagtcata
    27121 tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg gcaacatcta cagtctatag
    27181 tgccgtctgt tggatcttct tttgtttctc cccttctttt ctgctatagt aaagaagcaa
    27241 gatgattgta aaggagacga aatggatgat cgatagtcct gattattgta catgtaataa
    27301 caacaaaaat atccatacag aatttaatga ccaaggaagc ttgagcatat gcgacaactg
    27361 tcacaaacta gtcaaagatt cattta
//
LOCUS       DADVXO010000024        26182 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.025, whole genome shotgun sequence.
ACCESSION   DADVXO010000024 DADVXO010000000
VERSION     DADVXO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.025"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(211..579)
                     /locus_tag="KB950_000873"
     CDS             complement(211..579)
                     /locus_tag="KB950_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBB6857107.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            729..1211
                     /locus_tag="KB950_000874"
     CDS             729..1211
                     /locus_tag="KB950_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBB6857108.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1248..2258
                     /locus_tag="KB950_000875"
     CDS             1248..2258
                     /locus_tag="KB950_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBB6857109.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2373..3086)
                     /locus_tag="KB950_000876"
     CDS             complement(2373..3086)
                     /locus_tag="KB950_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857110.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3276..3866)
                     /locus_tag="KB950_000877"
     CDS             complement(3276..3866)
                     /locus_tag="KB950_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBB6857111.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4350..4577
                     /locus_tag="KB950_000878"
     CDS             4350..4577
                     /locus_tag="KB950_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBB6857112.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4593..5759
                     /locus_tag="KB950_000879"
     CDS             4593..5759
                     /locus_tag="KB950_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBB6857113.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5993..7036
                     /gene="hrcA"
                     /locus_tag="KB950_000880"
     CDS             5993..7036
                     /gene="hrcA"
                     /locus_tag="KB950_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBB6857114.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7130..7693
                     /gene="grpE"
                     /locus_tag="KB950_000881"
     CDS             7130..7693
                     /gene="grpE"
                     /locus_tag="KB950_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBB6857115.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7744..9573
                     /gene="dnaK"
                     /locus_tag="KB950_000882"
     CDS             7744..9573
                     /gene="dnaK"
                     /locus_tag="KB950_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBB6857116.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9724..10890
                     /gene="dnaJ"
                     /locus_tag="KB950_000883"
     CDS             9724..10890
                     /gene="dnaJ"
                     /locus_tag="KB950_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBB6857117.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10972..11637)
                     /locus_tag="KB950_000884"
     CDS             complement(10972..11637)
                     /locus_tag="KB950_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857118.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11613..11786
                     /locus_tag="KB950_000885"
     CDS             11613..11786
                     /locus_tag="KB950_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857119.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11831..12568)
                     /locus_tag="KB950_000886"
     CDS             complement(11831..12568)
                     /locus_tag="KB950_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6857120.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12570..14072)
                     /locus_tag="KB950_000887"
     CDS             complement(12570..14072)
                     /locus_tag="KB950_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBB6857121.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14331..16325
                     /gene="uvrB"
                     /locus_tag="KB950_000888"
     CDS             14331..16325
                     /gene="uvrB"
                     /locus_tag="KB950_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBB6857122.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16335..19151
                     /gene="uvrA"
                     /locus_tag="KB950_000889"
     CDS             16335..19151
                     /gene="uvrA"
                     /locus_tag="KB950_000889"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBB6857123.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19414..20679
                     /locus_tag="KB950_000890"
     CDS             19414..20679
                     /locus_tag="KB950_000890"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBB6857124.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20683..21414
                     /locus_tag="KB950_000891"
     CDS             20683..21414
                     /locus_tag="KB950_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBB6857125.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21549..22433
                     /gene="rapZ"
                     /locus_tag="KB950_000892"
     CDS             21549..22433
                     /gene="rapZ"
                     /locus_tag="KB950_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBB6857126.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22430..23428
                     /locus_tag="KB950_000893"
     CDS             22430..23428
                     /locus_tag="KB950_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBB6857127.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23462..24397
                     /gene="whiA"
                     /locus_tag="KB950_000894"
     CDS             23462..24397
                     /gene="whiA"
                     /locus_tag="KB950_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBB6857128.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24940..25590
                     /locus_tag="KB950_000895"
     CDS             24940..25590
                     /locus_tag="KB950_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857129.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25777..>26182
                     /locus_tag="KB950_000896"
     CDS             25777..>26182
                     /locus_tag="KB950_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBB6857130.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVS"
BASE COUNT         8645 a         4432 c         5572 g         7533 t
ORIGIN      
        1 attgctgaaa aaataatttt actaagtaga aaattcaaag ccattcaatc agtgattttt
       61 ttcagcacaa gaaagactcc ttataaaaat agcagatact ttgtcaatta agtgtttaac
      121 cagggatgat aaaaaggata ggattgaaca aaacctgtat aaggtgatga atagtttcct
      181 cgccagtaaa aggtttattc gtataaagct ttatcgccaa aatttatcat gacctttttt
      241 atcttcaata ttaaatgaaa tcaattgttc aatcaattcc cagttgatta cacttttcca
      301 aggaactttg atgacgttat ccgtatatac gtagcctgct tcttggatat ttttttcaag
      361 ggcacgaatc gctgcttttt ccggtgcgat agaaaaatga tttttagcaa aactaaatgc
      421 aataataaag gtaccatggt cagtaaacat aggttggttc cattttacag ttgtgtctag
      481 ctggggatac gtttctgtta tcttagcaaa aaggtctttg atcttttgtt gatgttcttt
      541 ttctttgatt tgattgatga tcggctgaaa tttttccatt ctaaaacgcc tccattaagt
      601 ggtttgattt attttaccag aaaaacaata gttatgtata aaaaacgaac aaaacacaag
      661 taatgttgta accgctatat ttatatgata agatgtctca aataataaaa tagaaaagag
      721 acataaaaat gattgacttg gcaaagaaat tcttacacga aaatatatat tttgagttgt
      781 atcatcatcg agccatttat acaaatgaag atgctttggt aataaagaaa gaacaaggat
      841 ttacaggtac agagaccaaa agcttattct taaaagataa aaaagataat aactacgtat
      901 atctgacttt tactacgaaa caaacggatt tcaaagcatt agggaagatc gtcggtaaaa
      961 ggctttctat tgtttcatca gaacgcatgg aagaacaaac gggacaaaag cagggcgctg
     1021 tttctccgtt tggttatgaa aaacagattc cgattattat tgatgaagag ctcttatcaa
     1081 atgaaaaact tgtttttgca ccaggtagac cagatcaaac gatggtcatc caaacggttg
     1141 acttaaacaa aatcatccag ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa
     1201 aaaacaatta accaagtaaa atagtgcata cttggaggtg aagcaacatg ataagttatc
     1261 cattattaga aagaaatgca acgattgggg ttactgcacc atcctcaggc gtatcagagg
     1321 aacaccattc gttactcgaa tcagctttaa agagaatgga agaaaaatct ttccaagtag
     1381 ttactggaaa gacgctttgg acgcaagata aggaaaaatc aggtccagca agaaaacgag
     1441 cagatgaatt caatcaaatg atgcaagatg aaaatatcca aatgatcttt ccgacttggg
     1501 gaggagaatt attgattgag attctcgatt ttgtcgattt tgatgctcta caaccaaaat
     1561 ggatagtagg ttattcagat attagtacgt tgctacttgc tgtcacatta aaaacaggga
     1621 tagccactgc acatggaact aaccttattg atttaagagg agagtacttg gatgaaacga
     1681 cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga atcgattcta cagcaatctt
     1741 ctgaaaagta tcaaaaagtg tggccccatg agcattcatc atcttctgtt ttccatctca
     1801 ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt aaggatgaag ggacgattat
     1861 taggtggttg tattgatacg atcaggcatt taatcggtac tccttatgga gatgtccaat
     1921 cttttaaaag aaagtataat gatggaaagc cgatcttgtg gtttctagaa aactgtgagc
     1981 tatcgtcagt tgatttaaga agatcacttg ttcagatgaa actggcaggc tggtttgatg
     2041 gcagctcggg gatcatgttc ggtagaagtg cggcagagga tttgttagat tataaaatga
     2101 aggatgtata cgaagatctg tcaaaggaat taaaactacc gatcttgtat gatattgatt
     2161 gtggtcatgt accgccacaa ctgacattga ttaatggtgc ctatgcagaa gtagcggtag
     2221 aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa aattttttcc tgccttggag
     2281 tctatcaaag accaataaat ctaaatagaa aagaaagtga aagaagctga caaaatgctg
     2341 tcagcttctt tttgtataac atattagaaa gtctaattat ctataactgt cacatcgatt
     2401 aactgtttca cgtcattggg atttcctgta ggaatagcta ctacagatgc attatcaatg
     2461 atttcttgag ttgtttctct aatgaacgct ggataagatg tttgatctgc aggcatttgc
     2521 caatgtgaag gaactggata actagtgatt gtcccgtcat ggttacttga atacactaaa
     2581 actccctggt aactgtattt cccagtgagc ataaccacat ctttcggata gactgcactc
     2641 cctgcatcat aaggggcgat aggtgttcct gcaggataat gaataacatg taattcgaaa
     2701 tcacccgatg tatctctaaa agtattacct ctaatagcta gccaaacacg tgcgtactca
     2761 atttgtagat cagaatatcc tgctaacgga tctattgact gagaagactg tgaggaggtt
     2821 gtgtctattg ttgaagaaga aatatttgtc tcaactgtac tactagatgt agaagaagta
     2881 ttgggctctg agactttact gcttgttgaa gatgtattcg tttcagtaga agctgatttc
     2941 tgagtacttg atttttcagc tgaactggtt tgtctgattg aacttttgtc tgtggtcgca
     3001 gatggatcac tattttcttt actaggagac gtacatccta caaggaataa agagaccaac
     3061 aataaaataa aacctaactt tttcatataa aatacctcat tattttttta ataccagtat
     3121 agcataatcc tgaatttttg aatccgtaaa accttgtcta tgaatataaa tagagtgtat
     3181 cttctttgat aaatgtcaga aaaagtgtga caaataatca tcagtaaaat tacttgtcac
     3241 actattgata aggagtattt aattgaagag aatgattagg agaaaggatc tccgtacttt
     3301 ttatagtaat catagaatcc aagtttgtat agacgaatcg ttttttctct tatttcttct
     3361 gtttcaaaat ggactgcttg aagaatcaag tctgtgaatt ctataggagc tgttccattc
     3421 gcagtaatca aattacgatc tttcacagct tgttgttcta taaaatcttc tttattctga
     3481 taattttccg attctaacca aggaaattgc gcatttccgg tgtgtttgta accttgcaat
     3541 aatccgctct gagctaaata atcaactgcc ccacaaatag cagctactgg tacgtctgtt
     3601 tttaacaatt tatcgattat ttgatataaa tgatgatttt ttatgtccca tgaattgcca
     3661 ccgatcataa taaaaaggtc aactttttca ggtatttgtt cgattaagta atcgattttt
     3721 gtttttaatc ctccaatgga agtgacctct gattggatag aagcagtttt gttttgccat
     3781 ttctcatttt ggtttagtgt gctagtaata taagcacctt cccaatcggc atattcctct
     3841 aatagaaacg taatcgcagt tttcattttc aagctctcct ttactatcta taacaaggac
     3901 aacagtttta acctacagac attcattaaa attgggtagc gacagcgaaa acaacaattc
     3961 ttaagtttta gacagagtat acagatagtc aacttaatta aatacgaact aaataaaaat
     4021 gacaggtaat catttttgtt taccagcatt ataacatttt gcatacatgg atgacaaaag
     4081 aaccaattcc cacttgcttg agtacaatat taaaagaaac tggaaggcaa aaagtaatta
     4141 tagaaacgaa aaataaatca tatcgggcac tcagacatga aaacaaccct agagatatat
     4201 aactaggtaa ccagtttaat gaaagaaaaa atcatccaag aagtcgattc ttggattttt
     4261 tagtggattt gttcgccaaa tacgtttagt atgcgtatat gagaggttgt aagcaaagcg
     4321 ttgtggtctg agcacttaag gaggaatctg tgaaaatagc ttttcatatt tttgcagatt
     4381 ttgacttaat gccgaaggtc agctttgcga ttaccgttta tcgagaggct gtaaaagcac
     4441 cgtgtagctc cgatgacaat aagcaagcat ttttagagct aggtttcctt gattctaaat
     4501 atgcggctta tttcaatgag ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc
     4561 acgacttttc gacgtaagga gaaagaattt caatgataga aacaataaat cccgcagaaa
     4621 acaaggtttc tgcgtacatt catatcccat tttgcgagca tatctgctat tactgtgatt
     4681 ttaataaagt ttttttagaa gggcagccag tagatgagta tgtggaaatg ctgctgaatg
     4741 aaatgaaaat aatgctagaa agtcatccga cagcgaattt agaaacactt tacgtagggg
     4801 gcgggacacc aacttcttta tctgctaaac aacttgatcg actgttatca ggtgctagag
     4861 agatcctgcc ttttcaaaat ggtaatgagt ttactgtaga agcgaatcca ggtgatttga
     4921 cccgtgaaaa acttcaagta atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc
     4981 agacatttga taatcgctta ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt
     5041 atcaaactat gcaattttta gaagcagaaa attttaccaa tgtaagcatc gatttgattt
     5101 atgcattacc gggacaaaca ctagaaggat atctggatac tttggaaaaa gctttggctt
     5161 tggatctgcc tcactattct ttgtactcat tgatcctcga aaacaaaaca atgttcatga
     5221 actgggtacg acaaggaaga ctcgaactgc ctgatcagga aacggaaacc agaatgtttg
     5281 aagagacgat tgaagcaatg gaaaagcatg gacgtcacca atatgaaata agtaactttg
     5341 ggctctccgg acatgaatct caacataacc tcatgtactg gaacaatgcc cactattttg
     5401 ggtttggagc tggtgctagc ggttatcttg gagatattcg ttaccgcaac aaaggaccaa
     5461 tccaacacta tttacgccca ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat
     5521 tgacacgaaa aaatcaaata gaagaagaaa tgttcttagg actaagaaaa aaaacaggga
     5581 ttactaaaaa acatttctat gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg
     5641 tgctaaggga attagagcaa gaagggttac ttgttaatga agaagaccgt gtttatttga
     5701 caccaagagg gacctttttg gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt
     5761 ttaaagaata tttgtttttt atctgaattt aaataaaaaa agttcaaaaa tacaataaag
     5821 gactattgaa acgttgatat aactggattt acatcaatta tttagaatta gcactcttgt
     5881 gtttagagtg ctaaaaaatg gagtctttct cttgacagga gaagggcttc ttgctatatt
     5941 tataagtgga ttagcactta gaatatatga gtgctaatga gaggtgagga agatgttaac
     6001 aaagcgacaa aacgacattt tacgtctgat catccaacac tatacgagta gtggcgtgcc
     6061 cgttggttcc aaaacattga tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa
     6121 tgatatgaaa gcattagaag atgaaggatt gctgttgaag actcactctt cttctgggcg
     6181 gattccatct gctttaggct atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt
     6241 agcagaagat gatttgctgc tgattcgtca gtctctaggc agagaatttc atgaaatcaa
     6301 tgagattatt aagcaatctg ctgagatatt atctgagctg acaagctata cgactttttc
     6361 attgggacct gaaataaaag accgccgact gactggcttt cgtatcgtac ccttgaataa
     6421 tcgacagatt attgctatcg tcgtaacaga tatggggaat gtagaaagcc aagtgttcac
     6481 tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg gtacagattg taaacgaccg
     6541 tctagtcgga gatcctctga tgacggtgta ccataaactt cgtacggaga ttccgatgat
     6601 tcttcataaa tatttccaaa cgacagaagg gatgtctcag ttatttgaca ccgttcttgg
     6661 tcatgttttt gaagataagg tttatgtcag tggacaaatg aatctcttgg attatgagcc
     6721 ccgtcaagat cttgatcagt tcaaatcgat gttttctttt atgaaagact cagacggact
     6781 tacacaaatg atcactccga tggatagcaa tattcatatc aagattggtg ctgaattagg
     6841 caatgattta ttgcagaata tgagtatgat tcaagcaagt tacgagatag atggtcatgg
     6901 aagaggtaca atagccttgt tagggcctac tagtatgcct tattctcgga tgttcggtct
     6961 agtcgatgtt tatcgaagag aactagcagc taaacttgct gattactacc gttcgcttga
     7021 cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc tctctttctt
     7081 agtaagagca atcaaacagc tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa
     7141 tcaagaagaa ctagataaag aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga
     7201 agctgcagaa gattcgggta tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt
     7261 aaaagctgag ttagaagaaa tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa
     7321 tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt cacaagattt
     7381 agccaaaaaa ttacttccat caattgacaa cctggaacgt gcattagcca cagaagtgtc
     7441 agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa
     7501 cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa
     7561 tcttcatcaa gcagtacaaa ctgttccagc gactgaagat acaccagccg atacgattgt
     7621 agaagtacta caagaaggct acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat
     7681 tgttgcacaa taattgtgcg gatataaaac ttacactttc aattaaagaa ggagacgtta
     7741 attatgagta aaattatcgg tattgactta ggtacgacta actctgcagt agcagtattg
     7801 gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa accgtacaac accttctgtt
     7861 gtatcattca aaaatggaga aatccaagta ggggaagttg caaaacgtca agctgtgaca
     7921 aacccaaata cgatttcatc aatcaaacgc catatcggtg aagcaggtta caaagtagat
     7981 gtagaaggta aatcatatac accacaagaa atctcagcaa tgatccttca atatatcaaa
     8041 ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct
     8101 tactttaatg atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa
     8161 gtagaacgta tcgtcaatga accaactgca gctgctcttg cctatggttt agataaaaca
     8221 gatcgcgacg aaaaaatctt agtattcgac cttggtggcg gtacttttga cgtatctatc
     8281 cttgaactag gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt
     8341 ggggacgact ttgataacaa aatcatcgac tacatggtag cagaattcaa aaaagaaaac
     8401 agcattgact tgtctcaaga caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa
     8461 gcgaaaaaag atttatctgg cgtatcaagc acacaaatca gcttgccatt tatcacagca
     8521 ggagaatctg gaccattgca tttggaaatg acattgacac gtgcaaaatt tgatgaatta
     8581 acagctgact tagtagaacg tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt
     8641 ttatcacaat cagaaatcga cgaagttatt ttagtcggtg gatctactcg tatccctgca
     8701 gttgtagaag cagtacgtaa agaaacagga aaagaaccaa acaaatcagt taaccctgat
     8761 gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga tcactggtga tgtgaaagat
     8821 gttgttttac ttgacgttac accattgtca ttaggtatcg aaacaatggg tggagtgttc
     8881 actaaattga ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt attctcaact
     8941 gcggcagata atcaaccagc agtagacatc catgtattac aaggtgaacg cccaatggct
     9001 gctgacaaca aaactctagg aagattccaa ttgacagata ttccagcagc tcctcgtggt
     9061 attccacaaa tcgaagtaac attcgatatt gataaaaacg ggattgtaaa tgtatctgcg
     9121 aaagatctag gtacacaaaa agaacaaaaa atcacgatca aatcttcttc aggtctaaca
     9181 gacgaagaaa tcgaacgtat ggtaaaagat gcagaagcaa atgcagaagc agataaagct
     9241 cgtaaagagg aagttgacct acgtaatgat gtagatgcat tgctattctc tgtggacaaa
     9301 acattgaaag aattagaagg caaagtggac gaagaagaag tgaaaaaagc agaaacagct
     9361 cgcgatgaat tgaaagccgc tgttgaagca aacaacatcg aagaaatgaa aactaaacgc
     9421 gatgcattga acgaaatcgt tcaaaacttg actgttaaac tatacgaaca agcggcacaa
     9481 caacaagctc aagaaaaccc agaagcagct caaggcggag cagatgatgt cgttgatgct
     9541 gattttgaag aagtcgatgg cgacgataaa taatcagttc actgaatttc gagagaaggc
     9601 caaagttgct actttggctt tttcttttga aaaatagttt gaagtatcgg tttttgtttc
     9661 aaacaaaata tgatatgata acaagcgacg tgaaaatgat gagaaccaat aggaggaaag
     9721 cctatggcaa caaaacgtga ttattatgaa gtcttagggc tctcaaaagg agcatcagaa
     9781 gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat accatcctga catcaataaa
     9841 gaacctgatg cggaagaaaa attcaaagaa gtatcagaag catatgagat tttaagtgac
     9901 ccgcaaaaac gcgctgcata tgaccaatac gggcatgcag gcacagatcc aaattacggt
     9961 gctggtggag ctggtggcgg atttggtgga ttcggcggct tctctggtgg cggcttcggt
    10021 ggttttgaag atatcttcga ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat
    10081 gcgcctcgtc aaggtgctga tttgcaatat acggtcgact tgtcttttga agaagcgatt
    10141 tttggtgtag aaaaagaaat caagtataat cgggaagaaa tctgtcatac ttgtggtggg
    10201 aacggtgcaa aaccaggcac acaaccaact acttgtcata aatgtcatgg tgcaggtacg
    10261 attaatgtag aacgccaaac accacttgga cgagtgatga gccgtcaaac ttgtgatgtt
    10321 tgtcatggga cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat
    10381 gagaagaaag cacattcagt taaagtgaat gtaccagctg gtgtagaaga aggacaacaa
    10441 atgcgtttag ctggtcaagg agaagctgga gaaaacggcg gaccatttgg tgatttgtat
    10501 gtggtcttcc gtgtagaaga aagtgatatc tttgatcgtg aaggttctga aatttactat
    10561 gaattgcctt taagttttgt acaagcagct ttgggggatg aagtccaagt accaactgtc
    10621 catggagatg tgaaattgaa gatccctgct ggaacacaga caggtacgaa cttccgttta
    10681 cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta
    10741 aaactgatca caccgaaaaa tctaaatgaa caacaaagag aagcattacg tgcattcgct
    10801 gaagctggcg gcatcaaagt tgaagaacag caagaagacg gtttcttcga taagtttaaa
    10861 gatgcttttg gcgggaaaaa gcggaagtag tttttctagt aaaaacgcta agtcattaat
    10921 aattaaaaac cagacgaaca atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg
    10981 taccaacaaa aaagccagct ttagaatatg ataatcgtca tagtttctag gggtataacc
    11041 agtgagagaa tggaacttgt tcaatcgata ttgcaaggtg tttttatgaa taaataattc
    11101 ttctgagcaa tttttgatgc ttccgttatt tttactgaag catgccaaag tcttcttgaa
    11161 caaatgtact tcttcttctg tcaggctttt caaaatacgt tttacatata atgttcgttg
    11221 atctgacggg attgctggta aaacgatccc gaggtctaaa tcttcaaaaa agcggatctc
    11281 gttcacagat tgatggaaag ctatccattc gcaaacttgt ttggcatgaa ggaaagattg
    11341 cggtgtctct tgctctgtat aacaaagcga cccaattcca aagtttagct gtaactgata
    11401 cattttatta aactttgtct ggattttatc aagcaatgat agcaatgagg ctctggaaat
    11461 ccgattgact aggataatga tttgttgtct atgtacagtg agcaaagcct gtccgtaata
    11521 agagaagacc ctttcgagca actccaataa aggattgatc cgttcttgac acaattgctg
    11581 attgtcagaa agttgtccca caactacata atatggagaa gaaaaatcaa gctcaattaa
    11641 gtgagggtgg tatcgactat gtacttgata tcatcaatga accgatggta ctttcttatg
    11701 cgatggaaca ggtagagcga ttgcttgttt gtgcaggaaa acaagcgatg cggctgatac
    11761 gattaggtga gaaaataaga agataaaaat acacggtccc ttcatggcga ccgtgcattt
    11821 ttggttttta ttaaatattt aatactttat tcaagaaatc ttttgtccgt tcattttgcg
    11881 gattttcaaa gatttgttga ggcgttccat cttctaaaaa gtttccgtcg tcaatgaaca
    11941 tgacgcgatt ggccacttct ttagcgaacc ccatttcgtg cgtaacgata accatcgtca
    12001 taccttgttc tgctaacttt ttcatgacgt tcaagacatc accgaccatt tcaggatcga
    12061 gtgcagaagt aggctcatca aaaagcatga tgtcagggtt catcgctaga gcacgagcaa
    12121 tagccacacg ctgtttttgt ccaccagaaa gcatttcagg atacatatct tttttatctt
    12181 ctagaccaac tgtctgcagt aatttgattg cttttttctc tgcttcttct ctagattctt
    12241 tcttcaggtc catcggtgct aaaatgatgt tttccaaaat agaaagatga gggaataggt
    12301 taaagtgttg gaataccatt ccgatatgtt gtctcaccaa gttgatatct gtgcttttat
    12361 ccatcaagtg ggcgccgtca ataataattt caccgtcaga aggttcttct aaacgattca
    12421 agcaacgcaa gaatgtactt tttcctgaac ctgaagggcc aatgatacag acgacatctc
    12481 cttcgttgat ggatacattg atgtcgttta atactgtgtt atcgccgtat tttttgacta
    12541 aatgctcaac taagattttt tctgccatct tacttcacct tcttttctaa aacttttgct
    12601 aattttgtca atgctgtgat caaaatcaag tacatgatcg caatgatcaa gtaaacatac
    12661 gtactttgtg ttgtacgtgc aacaatgatc ttaccagttt gcagcaattc gactacaccg
    12721 attgcggaaa tgatcgttgt gtcttttaat gagataacga actgattgac gaatgatggg
    12781 atcatgatgc gtatcgcttg cggcaaaatg attttttgca ttgtccgatt ataagaaaga
    12841 cctaaacttc tggaagcttc catttgtccg acaggaacgg cgttgatccc gcctcgaaca
    12901 atctctgcga tataagcact tgcgttaaga gtcaaggtga taacaccagc aaggaagtca
    12961 ggaatcgtaa atccaacgat accaggcaac ccaaagaaaa taaagaacgc aagaaccatc
    13021 attggaattc ctcggatgat atcgacataa atactagcaa ttgtgcgaag agcgcgtatt
    13081 ggtgctacac tgaataatcc aaaaatcaca ccgattacta tagccaaagc aaaggagatc
    13141 aaagttagga gaattgttct ccaaagtcct ttcaacaatg cttgatagtt gttttggata
    13201 agtccaagaa atgttgattc atcagcagtg gaagactcac tggaagtttc tgaaccatcc
    13261 gctacatatt ctttaataat cttatcgtat tcgcctgttc tttttaattc tttcaatcct
    13321 tcattgaaca tattaagaag ctcaggattt tgtccttttt taacggcaaa tgcatatttt
    13381 ccgccagatt cttttttgat cggtgtttcc aattcttgtc cttgtttgat cgcataaccg
    13441 attaccggat aatcatccat cattgcatcg attgcgccaa cttttaaccc gtcgtacaat
    13501 tgatcagcag catcaaaata tttgatagaa tagccatatt cagattcatg gtcgcttaaa
    13561 aattcagcac tttcggtacc aacttttgct ccgactgttt ttcctttcaa atcttcataa
    13621 gacttgatcg aagtatctcc ttttttgaca gctaattgga tcccgctatc aaagtaagaa
    13681 tcagaaaaat catagctttt ctttcgttca tctgttacag tcattccggc aatcattccg
    13741 tctgcttgtc ctgattccaa agcttgcaaa gcagcagaga aaccacggta gtccattcgg
    13801 atgttaaatc cttgcagttt ggcgatgttt tccatcaatt ttacatcgat gccttcgtac
    13861 tcgccctttg cgttttgaaa ttcaaatgga gcaaaagcag agtcactggc aattacgtat
    13921 acgtcttttt tgggctctat tttttgcatt gtctcgcttg tgcttgactc tgccgttgta
    13981 gtgttgtcag cttgtgcaac atgaccaaac gcaagtgtca caagtcctgt cattaagaaa
    14041 acaaatgagt gaaataaatg ctttcttttc ataatcaacc tcctaaattt ccaatatttt
    14101 attattatag gcgaaaagtg ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa
    14161 atgcgagttt ctaacatttt atgttataaa acataacatg tataataaga taaaaaagtg
    14221 gcgaaagtat gttcggtgtg gtataatttt ttagtaagga atgcctttgc tttccttttc
    14281 tttttattgg tcgtaaaatg aaaaggaatg aatgaaaagg aggacgaaga atgattgaga
    14341 gagatacctc ccgacacttt gagcttgtct cgaaatacca accggctggt gatcagcctg
    14401 aagcgattaa ccaattagtg gatggcgtag tcggcgggaa aaaagcacaa atcttattag
    14461 gtgcgactgg aacagggaaa acatatacga tttccaatct tattgagaaa gtgaataaac
    14521 caacactgat tattgcccat aacaaaacat tggctggtca gctgtacggg gaattcaagg
    14581 aatttttccc taacaatgct gttgaatatt ttgtttctta ttatgattat tatcagcctg
    14641 aagcttatgt gccttcaagc gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga
    14701 tcgacaaatt acgacattcg gctactagtt cattattaga aagaaatgat gtgatcgtca
    14761 ttgcatccgt ttcatgtatt tttggattag gctcgccttt tgagtaccaa aaacaagtag
    14821 tttcgattcg acagggagca gaactagatc gcaatcagtt gattcgtgac ttagtcagta
    14881 tccaattcga aagaaatgat atagattttc agcgcggtcg ttttcgagtg cgcggtgatg
    14941 tcgtggagat ttttccggct tcaagagatg aacgtgcctt acgtgtggag ttttttggag
    15001 atgaggtcga acgtatccga gaagtcaatg cactgacagg tgaagtttta ggagaaacgg
    15061 aacacgtagc aatcttcccg gcgactcact ttgtgacgaa tgatgaacat atggagcatg
    15121 ccgtagcgaa tatcaaagcg gaattagaac agcgtttaac tgtcttgcga aatgaaaaca
    15181 aacttctgga agctcaacgt ttagaacagc gaaccaatta cgatattgaa atgatgttag
    15241 aaatgggcta cacgtcggga attgaaaatt attctcggca tatggacgga cgtaaagaag
    15301 gtgaaccgcc ttatacgcta ttggatttct ttcctgaaga ttttttgatc gtggcggacg
    15361 aatcgcacgt aaccatgcct caaatcagag gaatgtataa tggtgaccga gcgagaaaac
    15421 agatgctagt agattacggt ttccgtttgc catctgcttt agacaatcgg ccacttcggt
    15481 tagaagagtt tgaaaaacat gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat
    15541 atgaacatga acaaacagat accgttatcc aacaaatcat ccgtccgaca gggctgcttg
    15601 acccagtgat cgaagttcgt ccaatcatgg gacaaatcga tgatctagta ggcgaaataa
    15661 atgaacgagt cgaaaaagac cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag
    15721 aagacctcac agattatttc aaagaattag ggatcaaagt caaatatttg catagtgata
    15781 tcaaaacgct ggaacgaaca gaaatcattc gagatcttcg attaggtgaa tttgatgtgt
    15841 tagttggaat caatttgctt cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta
    15901 tcttggatgc ggataaagaa ggctttttac gtagtgaacg ttccttagtc caaacgattg
    15961 gacgagctgc tcggaatgaa gaaggtaaag taatcatgta cgcagacaaa gtaacagatt
    16021 ctatgcgtct agcgatggat gaaacgtcaa gacgtcgtac gattcagcag aagtacaatg
    16081 aagaacatgg gatcgttcca aaaacgatca ttaaagaaat ccgagatttg atttctatta
    16141 cgaaagaatc ggaagatgat acaaaagaag cggtacaagt ctcgtacgaa gaaatgacga
    16201 aggaagaaaa agacacgtta ttgatgaaat tagaaaaaga aatgaaagat gcggcaaaag
    16261 ctttggattt tgaaacagcc gcgaatgtgc gagatatgat tttagaatta aaagcttcaa
    16321 agtaggagga caacatggct aacgataaaa ttacgattca tggtgcacgt gcccataact
    16381 tgaaaaatat agatgtaacg attccacgag ataaattcgt ggtcgttaca ggtttgtccg
    16441 gttctggaaa aagttcccta gcatttgata cgctgtacgc tgaagggcag cggagatacg
    16501 tagagagcct ttctgcttat gctcgtcagt ttttaggaca aatggataag ccggacgtag
    16561 atagcatcga cggattgagt ccggcgattt ctattgatca gaaaacaaca agtaaaaatc
    16621 ctcggtcgac agtaggtact gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac
    16681 gtgtaggaca tccgatttgt cccaacgatc atatcgagat taccagtcaa tcagtagatc
    16741 aaatggcaga tcaagtaatg gctttgcctg agcgcacaaa gatccaagtg ttggcaccga
    16801 tcgtggtaaa gaaaaaagga caacataaaa aaatattcga acgaatccaa aaagagggat
    16861 acgtccgcgt tcgagtcgat ggtgagatct atgatttaag tgaagtgcct gagctggaga
    16921 aaaacaaaaa acatgatatc gcgatcgtca tcgaccggat cattgtgaaa gaagggattc
    16981 gttctcgcct atttgattct ttagaggcgg ccctgcgatt agcagacggc tatgctctgg
    17041 ttgacgtgat cggtgaagaa gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg
    17101 gatttactgt tggcgaacta gaaccaagac tgttctcatt caatgcgcct tttggagctt
    17161 gtccagactg tgatggttta ggtgtcaaat tagaagtcga tcaagattta gtgattccag
    17221 atagaacgaa gacacttagt caaggtgcta tcgttccatg gaatccaatc agctctcagt
    17281 attatcctca aatgttagcg caggcttgtg aaagtttcgg tatcgattta gatacaccat
    17341 tcgaagaatt accggaagag catcaggaga tcatcttgaa tgggacagat gatcttttcc
    17401 atttccatta tgaaaatgat tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg
    17461 tattgaagaa tatcgagcgt cgttatcatg atacgaacag tgatttcacg cgtgagcaaa
    17521 tgcgtcttta tatgacagaa ttaacttgtc agacatgtaa aggttatcga ttgaatcccc
    17581 aagctctatc tgttcaaatc aatggaacga atatcgggca aacaaatgag ttgtctattc
    17641 aaaaagcagt agatttcttc tcaaacttaa cgttatctga acaagagaaa gtaattgcca
    17701 aaccgatttt aaaagaagta aatgacagac tttcattctt ggaaaatgtg ggactagact
    17761 atctaacttt gagtcgcgct tccggaacat tatctggggg agaagcgcag cgaatcagat
    17821 tggctacaca aattggatcg aatctttctg gagttttgta tattttagat gaaccatcga
    17881 tcggattgca tcaacgcgac aacgatcgac tgattggctc attaaagaaa atgcgtgatc
    17941 ttggcaacac cctcgttgta gtcgaacatg atgaagatac gatgtgggca gcagattatt
    18001 tgattgacgt cggacctggg gccggagaac aaggcggaca aatcgtggct gcaggcactc
    18061 ctgaagaagt agctaatgat gaaaattctt tgacagggaa atatctttca gggaaaaaat
    18121 caatccctgt accaaaagaa cgccgtaaag gtaatgggaa agcaatcaag atcactggtg
    18181 cttcagaaaa caatctgaaa aatattagtg ttgaatttcc attaggagaa tttgtagcag
    18241 tcacaggtgt gtctggttct ggaaagagta cattggtcaa tagtatctta aagaaatcgt
    18301 tagcgcaaaa attaaataag aattctgcta agccaggtaa attcaagaca atttccggct
    18361 acgaaagtat cgaaaagatc atcgatatcg atcaaagccc aatcggccgg acgccgagaa
    18421 gtaatccagc gacttataca agtgtatttg atgatatccg tgggttattt gctcaaacga
    18481 acgaggcaaa aatgcggggt tataagaaag ggcgttttag tttcaacgta aaaggcggtc
    18541 gttgtgaagc ttgtcgcggg gatggaatta ttaagatcga aatgcacttt ttgcctgatg
    18601 tctatgttcc ttgtgaagta tgtcatggta aacgatataa ttctgaaaca ttagaagtgc
    18661 attacaaagg aaaaagcatt gctgatattt tggaaatgac agtagaggat gctgtagaat
    18721 tcttcaagca cattccaaag attcatcgca aactgcaaac gattgttgat gttggcttag
    18781 gttatgtgac gatggggcaa ccagcaacga cattgtccgg tggtgaggca caacggatga
    18841 aacttgccag tgaattgcac aaaatctcta atggaaagaa tttctatata ctagatgaac
    18901 caacgacagg acttcatagc gatgacatcg cccgcttgtt gcatgtatta caaagattag
    18961 tagatgctgg taacacagtt ttagtgattg aacacaatct agatgtaatc aaaacagcag
    19021 attatatcat tgatttagga ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg
    19081 gaacaccaga agaaatcatt aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa
    19141 taatggtata aagaggatag atagctgaga tagttaaaaa aacataaaaa aatgaatttt
    19201 tgcttgtttt attaatatca gtgttctaga atcattacat ggtagttctt tctacactga
    19261 ttggtataga taaaaacttc ctataagttc aatccgttca aaaaagaatt gagcaatcgg
    19321 aggactttcc tttgattgct tttttcttta tggtatgata ttccttgtgt agttggaaaa
    19381 gaaaaacaag agattatgag taagtgagag ataatgatga acagtattga aaatgaagaa
    19441 tttatcccta tattattagg aagcgacatg aacgtttatg gtatggcacg ttcttttaat
    19501 gaagcatacg gcaaaatctg ccaagcatat gcttcggatc agttagcccc tacccgctac
    19561 agtaaaattg tcaatgtaga agttattcca ggatttgata aagaccctgt atttatcgag
    19621 acgatgcttc gtttggcaaa agaaagatat tctgataaga gtaaaaaata tcttttgatt
    19681 gcttgtggtg atggatatgc agaattgatc tcccaacata aacaagagct gtcagaatat
    19741 tttatctgtc catatattga ttattcttta tttgaaaggc tgatcaataa agtaagcttt
    19801 tatgaagttt gtgaagaata tgatcttccg tatcctaaaa cattgatcgt cagagaagaa
    19861 atgttagtaa atggacattt ggaacaggaa ttgccttttg aattcccagt tgctttgaaa
    19921 cctgcaaaca gtgtggagta tctttctgtc caatttgaag gaagaaaaaa agcttttatt
    19981 ttagagacta gagaagagtt cgatttaatt ttaggccgga tatacgaagc cggttataaa
    20041 agtgaaatga ttgtacaaga ttttatcccg ggtgatgata gtaatatgcg tgtgctaaat
    20101 gcgtatgtag atgaagatca tcaagttcgg atgatgtgcc ttggtcatcc actattagaa
    20161 gatcctacac cagcatcgat tggtaattac gttgtgatca tgccagatta taacgaaaaa
    20221 atctatcaaa ccattaaggc atttttggaa aaaatcgaat atacaggctt tgccaatttc
    20281 gatatgaagt atgatcctag agatggagaa tataaattat ttgaaatcaa tttgagacaa
    20341 ggtcgtagca gtttctttgt gacattgaac ggcttgaatc tggctcgttt tgtcacagaa
    20401 gatcgagtgt tcaacaaacc ctttgttgaa acgacctatg gaacgaatca atcagataaa
    20461 gcaagattat ggatgggtgt gcccaaaaaa atctttttag aatatgctcg ggaaaatgaa
    20521 gacaaaaaac tagctgagca aatgatcaaa gaaaatcgtt acggtactac tgtattttat
    20581 gaaaaagacc gatcaatcaa gcgctggttg ttgatgaaat atatgttcca taactatatt
    20641 ccaagattca aaaagtattt tcatgtgaaa gaaggctagt tcatggagaa ttttttcagt
    20701 attttaggcg gaatgggcac gatggcgaca gaaagttttg ttcgcttgat caaccaccga
    20761 acgaaagcta caaaagatca agaatatttg aattatgtct tattcaatca tgcaacagtt
    20821 cctgatcgta cagcttatat tttagatcga tcagaagaaa atccaatgcc gtttctgctg
    20881 gatgatattg agaaacaaaa tttattgcga ccaaacttca ttgtgttgac ttgtaacacc
    20941 gcacactact tttttgaaga gttacaagcg gcaacggata ttcctatctt gcatatgcca
    21001 agagaagcag caaatgaact tgtccgtcag catacgacag ggagagtagc gattctagga
    21061 acagaaggaa gtatgaaagc tggaatctat gaacgcgaag tcaaaaatct tggtttcgaa
    21121 acagtgattc ctgatactgc gcttcaagaa aaaatcaatt acctgatcta tcatgagatc
    21181 aaggaatcgg attatttgaa tcaagagctc tattacgaaa tcttagagga agcggtagag
    21241 cgcctaaact gtgaaaaagt catcctaggt tgcacagaat tatctttgat gcatgaattt
    21301 gctgaagata atcattaccc agtgatcgat gctcaatcta ttttagcgga tcgaacgatc
    21361 gaacgtgcat tagctgaaag aagcgaggca ttggatacag catcggaaaa gtaaaaaaat
    21421 tggatttggg gcaaatatcc taaaagatat ttggtccaaa tctttttgta aaagaaaaag
    21481 aaaactggta atgatacgcc cgggatgata taattttatt atgcacaatg tgagaggggt
    21541 tcgtaaatat gacagataat ttgcaattag tcatcataac aggtatgagt ggtgcaggga
    21601 aaacagtagc tatccaaagt tttgaagata tgggctattt ttgtatcgat aatatgccgc
    21661 ctcgattgat tccaaaattc tgggaattga tcaaagaatc aggtaaagtc acaaaaatgg
    21721 ctttaggagt ggatttgcgt tctcgttcct tctttgaaga aatccaaaac atgctgatcg
    21781 aaatcgaaaa tacgaacttc atcgacactt ctatcttatt tttagatgct tcagacgaag
    21841 aactagtggc tcgctacaaa gaaacaagac gagcacatcc aatggcgatg gacggtttga
    21901 tcactgaagg gatacgtaaa gaacgggcga tactagacga cttgaaagcc caagcttccg
    21961 tgatcattga tacgacaaca ttgacaccac gacaattacg tgaaaaaatc aatgagtcat
    22021 tcaaatcaaa gggagaccaa ggatttcgga tcgaatttgt atcatttggt tttaaatacg
    22081 ggttgccgat cgattcggat attgtgatgg atgtgcgttt tttgcccaac cctcattata
    22141 tcccggaact aagaccattg accggaaaag atcagccagt ctatgattat gtgatgtcct
    22201 ttgatgaaac gcaatcattc taccataaat ttttggatct tctggaaact gtattgcctg
    22261 gatacattaa agaaggaaaa agcagcttga cagttgcaat cggctgtaca ggcggtcaac
    22321 accgttctgt ggcattgact gaacgtttgg caaatgcttt agggaaaaac tataaagtca
    22381 atgtgacaca tcgggacaag gataaaagga aagagacggt gaaccggtcg tgaaaatgaa
    22441 aacctaccgg ataagaaaac caaagatcgt agtggtcggc ggtggcactg gtctgccagt
    22501 tatcctgaaa agtttacgaa atcaaggagc agatatcaca gcagtagtga ctgttgcaga
    22561 tgatggtgga agcagcggtg caatcagaga atcgatcgct atggctcctc ctggtgactt
    22621 gagaaatgtc ttagtggcgt tatcggatat gccacaattc tatgaagata ttttccaata
    22681 ccgtttcaaa aaggaagatc agtttttagc caatcatacg atcggtaatc tgatcattgc
    22741 cgcagtttcc gagatgcggg gaagcactta tgaggcgatt caattattag caaaaatgat
    22801 gcatgtagat ggacatgttt atccttcttc agaaactcct ttgaccttgc atgctgtttt
    22861 taaagacgga acgactgcag taggagaatc taaaatcgcg atcaaccgta aaaccattga
    22921 ccgtgtgtat gtctgcaaca caaatgataa aagtgaagcc aaagcggctc gaaaagtcgt
    22981 ttcagcaatt atggacgccg acatggtcgt tttgggaccc ggaagtttat tcaccagtat
    23041 ccttccaaat ttagtgatac cagaaatcgg cgaagcaatg ctcaagacaa ctgctgaaac
    23101 cgtctatatc tgtaatatca tgacccaaaa aggggaaacg gaacatttca cagatgccga
    23161 tcatatacgt gttttacaca agcatttgaa tcagcctttt atcgatacag tattggtcaa
    23221 tacggaaaaa gtgcctgaag attatatgga cccagagatc tatgatgagt atctagtaca
    23281 agtcgcacac gacttcaacg gattgagaaa tgaaggatgt cgagtgattt ccaccgactt
    23341 tttagaattg aaagatggtg gtgtcttcca cgatggagac aaagtagtag aagaattgtt
    23401 ccgtttagtt ttcggttcaa aatattaagc aatttattgt tggagaaagg aggagaatag
    23461 gatgtcattt gctgcagatg tgaagaaaga actaactggt ttggaagttc atcgagaaca
    23521 tgcgaaagcg gaattagctg ctttgctgcg aatgaacggc tctttaggta ttgtgaacca
    23581 gcagtttatt ttgaacgtcc aaacagaaaa tgctgcaatt gccagacgta tctattctct
    23641 tttgaaagat cattatcagg tacgaagtga gctactcgtt cgtaaaaaaa tgaaattaaa
    23701 aaagaacaat gtctatatcg tccggttgaa gcaaggcaca aaagatgtgt tgatggatct
    23761 tgatatcatg gatggtatga tgttcaatgg acacgtttcg gacgagatta tgggaaattc
    23821 gcaaaaaatg cgttcttact tacgtggtgc ttttatggcc tcaggttcag tcaataatcc
    23881 ggaaacgagt cgttatcatt tggaaatatt ttctatttat gaacagcata accaagacat
    23941 ttgcgatatg ttaaattatt acggtctaca tgctcgaacg cttgaacggc gcaatggcta
    24001 tatttcgtat ttaaaaggag cagaaaaaat tgctgatttc ttaacattga ttggtgcaac
    24061 caactcaatg ctccgatttg aagatgttcg aatcgtacgg gatatgagaa attcggtcaa
    24121 tcgtctagtc aattgtgaga cagcaaatct gaacaagacg attgatgcgg cttctaaaca
    24181 aatcgaaaac atcgaattga tcgaagcaaa ggtcggtcta catgcccttc ctgaaaaact
    24241 tcaggaaatc gcagaacttc ggcttcaaca tccagaggtc agcttgaaag aattagggga
    24301 aatgattccc tcaggtgcaa tatcaaaatc gggaattaat caccgaattc gcaaaataaa
    24361 tgaatttgcg gaaaaactgc gtgaagaagc agtttaatcc aaagaaaaaa ctgatacatg
    24421 aagcaggaga tgcgaatgta tcagtttttt ttaaaagcta tacttgcttt tcttccgtca
    24481 aactataatt ccaactttac tggagtaaca taatgttgaa aggcttctat tgttttttaa
    24541 tacaatcgta taaaaagcgg ggagacaact gaatgaagat tttagtttta tacataaact
    24601 tttgcttaat atagaagtcc tgatatagat aggatcaagt atttatctat aactggagat
    24661 taattaaaaa ctggaaggaa ctgctcgatc aaccttgaga actgtttttc ttacttctgg
    24721 tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat tagctagtcg
    24781 aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg atttattcag
    24841 tgataatcat aagttcataa atagtaattg ggatgtatgg ttggtatacg ttatacctga
    24901 acttgaacta tcaagatttg gtttagaatt tgagaatcga tgtttataaa gggagggcaa
    24961 aaaatgtgct tttttacaac ttttactatc attatgattg cagtactttt tatttttcta
    25021 gatatagcaa agcgaaatac agcattttta ttatatcggg ttttacttcg agcaggtctc
    25081 ataacattta tatcgatcgt tggatttttt ctattcacag ttattgtatt tatttggaga
    25141 actacagcac caccattgcc agagattaca tatggagaat ttccgtttag tcttgaatat
    25201 gaaatagatg aggaaagaat tttgattgaa gatacggtga ttgcagaatt tataggaagt
    25261 tttagaggga atgtgacaac tcaacctaga cgaagttgga gtataaaact tttaaataat
    25321 agtggagagt tgaaagcatt tcattatgaa ttcttaataa tagaaagtga agatgtaaca
    25381 atcactttaa gtagtggctc tgctggatat tttatgaatg atggtggaag caagccacag
    25441 ctcacagtcc gtgatcgtat tgcaggtttt atgcattttt ctcactatga cttagaagaa
    25501 gctcgtaaaa tacttgcaga atatgggatc acattaatca attgggaata tgcgccaccg
    25561 ataagaaatt ttttcaatga ttattattag tcttataaaa aacagaatgt aaaatttatg
    25621 tgcgcgagta tttagttttc cattttttgt ttgatagata tatagcattg ttgaagcatg
    25681 ggatagtggt gccaatttta tcaatgaggt tggtcaggca gttaacataa ttggtgatgc
    25741 tataaatcca tttaattgga attggaggaa ttaggtgtgg aaacagtgag attatggata
    25801 ttcatgaaaa attttcgttc ggctttgaga tatgttattc tggaatcaaa atcaggttat
    25861 tatctgattg acacagatca ctatactttt tggggaggct acctttttcc gatttttaat
    25921 tggataatcc cccataaggt tatgaaaatt aacttgcctg cagaagaaat aaatagatta
    25981 atactcaaca aagatttaca aaggaaaaga gagaaagaac attctaatgt tgcttggata
    26041 ggtccagcat ttgcagtagt gacttcttca attgtcggtc ctatgctaga gagtatttat
    26101 agtgattttt ctattggaat acgttattta tttgttactt taagtatagc tgttcttttg
    26161 tgcataaagg gttatgttag ca
//
LOCUS       DADVXO010000025        26092 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.026, whole genome shotgun sequence.
ACCESSION   DADVXO010000025 DADVXO010000000
VERSION     DADVXO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.026"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..122)
                     /locus_tag="KB950_000897"
     CDS             complement(<1..122)
                     /locus_tag="KB950_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBB6857131.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKL"
     gene            complement(464..1099)
                     /locus_tag="KB950_000898"
     CDS             complement(464..1099)
                     /locus_tag="KB950_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6857132.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1611..2753
                     /locus_tag="KB950_000899"
     CDS             1611..2753
                     /locus_tag="KB950_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBB6857133.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            2746..2952
                     /locus_tag="KB950_000900"
     CDS             2746..2952
                     /locus_tag="KB950_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB6857134.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3031..4224
                     /locus_tag="KB950_000901"
     CDS             3031..4224
                     /locus_tag="KB950_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6857135.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            4429..5568
                     /locus_tag="KB950_000902"
     CDS             4429..5568
                     /locus_tag="KB950_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBB6857136.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5561..6361
                     /locus_tag="KB950_000903"
     CDS             5561..6361
                     /locus_tag="KB950_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6857137.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6361..7188
                     /locus_tag="KB950_000904"
     CDS             6361..7188
                     /locus_tag="KB950_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6857138.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7166..7900
                     /locus_tag="KB950_000905"
     CDS             7166..7900
                     /locus_tag="KB950_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6857139.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8000..8866
                     /gene="rfbA"
                     /locus_tag="KB950_000906"
     CDS             8000..8866
                     /gene="rfbA"
                     /locus_tag="KB950_000906"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBB6857140.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            8880..9452
                     /gene="rfbC"
                     /locus_tag="KB950_000907"
     CDS             8880..9452
                     /gene="rfbC"
                     /locus_tag="KB950_000907"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBB6857141.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9474..10502
                     /gene="rfbB"
                     /locus_tag="KB950_000908"
     CDS             9474..10502
                     /gene="rfbB"
                     /locus_tag="KB950_000908"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBB6857142.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10600..11451
                     /gene="rfbD"
                     /locus_tag="KB950_000909"
     CDS             10600..11451
                     /gene="rfbD"
                     /locus_tag="KB950_000909"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBB6857143.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11485..13518
                     /locus_tag="KB950_000910"
     CDS             11485..13518
                     /locus_tag="KB950_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857144.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13562..14842
                     /locus_tag="KB950_000911"
     CDS             13562..14842
                     /locus_tag="KB950_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBB6857145.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15052..15858
                     /locus_tag="KB950_000912"
     CDS             15052..15858
                     /locus_tag="KB950_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857146.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            15870..17090
                     /locus_tag="KB950_000913"
     CDS             15870..17090
                     /locus_tag="KB950_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857147.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17080..18666
                     /locus_tag="KB950_000914"
     CDS             17080..18666
                     /locus_tag="KB950_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6857148.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18705..20843
                     /locus_tag="KB950_000915"
     CDS             18705..20843
                     /locus_tag="KB950_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6857149.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21211..22194)
                     /locus_tag="KB950_000916"
     CDS             complement(21211..22194)
                     /locus_tag="KB950_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBB6857150.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22572..23963
                     /locus_tag="KB950_000917"
     CDS             22572..23963
                     /locus_tag="KB950_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBB6857151.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            23979..25019
                     /locus_tag="KB950_000918"
     CDS             23979..25019
                     /locus_tag="KB950_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6857152.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25038..>26092
                     /locus_tag="KB950_000919"
     CDS             25038..>26092
                     /locus_tag="KB950_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBB6857153.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTE"
BASE COUNT         8449 a         4403 c         5329 g         7911 t
ORIGIN      
        1 agttttgttt cattgataat atcttgcaat gatgtgttat catatccttt ttcacaaaac
       61 atttttgtag aaacatctaa aatgagtttt actgtttctt ctggatattt atttcgtgcc
      121 ataacttacc tccaatacaa accgttggtc tgattaaagt atatcttagt attttatgct
      181 tgtcaatata aagacttgca attttataaa aatgcagaat gcaagccttt ttcatttatt
      241 ctacaatctt ccagttgctt tccttatgcg gtgtcaattc atactgtgat acctgcgttg
      301 cctttgcctg atttaccaca aatcagattt tatccctttc ttcctcttga ttgcccacaa
      361 cagaaaagaa aaagccctgt caagagcttt gccaccattg gtggtgcttt gcactcttga
      421 tcgggctttt tgtttgctgt atcttctcgc aagaggaaaa aagttactcg tcctcatcat
      481 caaaattgtt attccttaat acttctcgta acagttccat atcttctttt gagttcgggt
      541 ttatcatttt cacgacttga taaggtgtct tatacttatg tccctctatg ctttccccaa
      601 acatatccat actgtataaa tcatcatagc ggtctaataa tttttgaaac ttcaaatgct
      661 ggataaattc tttgattggg taaaggtcgg acgcttcaat gcttttcccg ttgatatagt
      721 ctgtaatata ttcccgtaac ttttctaact catattccag ccattcttcc tcgctgtcaa
      781 agaagttatc atagtgtcca tgtttcagtt ctgcatttag acgttcttcg gctcttaaaa
      841 actgacagac ttccttttca gattgattga catacttcca ttctcccgac aacaattcat
      901 tgggcgtaac gtccagcact tccattatct tttccagcgt ttcataggca ggatagttca
      961 accctctttc aatcttggaa aggctctgca tattgatacc gattttgtcc gcaagttcct
     1021 gttgtttcat tcccctaaat tttcttatgg tctgtatatt atttcctaaa tggcttattt
     1081 tcattgcata accctccata tctcgttata tcttcgctat tataagtcta tcatgcttaa
     1141 tatgatttgt aaaggcaaaa aaagatattg acaaataagc atataagaat tactatgttc
     1201 ttgtaatcga ataagcacaa tagaatgaaa tataaaaaca ttcaaaaaga aatacctatc
     1261 tgattaaatc cgtagatatg aatatcggtt gaaatggaac ttgttagaaa aggcaaaaca
     1321 gaacttgctg acaaagtatt tctgacacgg tggcggaagc ctctcaggct taccgccatg
     1381 acaccttgtg tcaaggggaa gaaatgcttt gtcggctggc gagcctgcga gccttgtctg
     1441 tgcccccgtt atctaacagg ggggcactat ttcacaaaag acaagtcaaa aaccatacac
     1501 ccccaaacct gtgagtagta atgagaggtt ttaagaagat agtacatatc acactttttg
     1561 aaaggtggtt tacaggtatt tcaaggctat tgtgggattg gaggaataca atgaacaatt
     1621 tagaatttgc acttgaaatg aagaacaaac gtcttgccag cggtctttca caaggagaac
     1681 ttgcaagtct tacccatgta tcaagatata gtatcaaccg ctttgagaac ggaaaagcta
     1741 acgccagcaa ggaaacacag aacattatcc tgcgttgtct gaattactat atctgcgata
     1801 agccttttta tctgctcgtt gattatctgt ctgtccgttt tccaacgact gacgcattgg
     1861 aagttatccg aaaggtgctt ggcatgaaag cagattattt tatccattat gactatgggt
     1921 attatggcta taaggagcat tatgcttacg gggaaatcaa ggtcatggct tctgatgatg
     1981 aacacatggg cgtattttta gaattaaaag gggcaggctc acgcaacatg gaatatgtct
     2041 tacaggcaca gaacagggac tggtattcat tcttaaaccg ctgtcttgac tgtggcggtg
     2101 ttatccgacg ttttgacttg gcaatcaatg atatgtgcgg tctgctggat attcctgtgc
     2161 tatctgaaaa atacagaaat ggtggtgcgg aatgtcgctg taaaaactat gaaaatgtac
     2221 aaggtggaaa attaagcgga aaaaaccgta atttagcaag taccctttat atcggctcaa
     2281 aggcaagcac aaaatatttc tgcctgtatg aaaaacaaaa ggaacaggca acgaaaaaga
     2341 aacataccga tattatcaac cgttttgaaa ttcgtctgcg tgacaaaaag gcggtacagg
     2401 cagttgaaga actgttattg acgtataacc ctcatgggct ggtgttttat ctcattaccg
     2461 attttgtgga atttcctgac tatccattgt gggagatatt catttcccat gacagtttac
     2521 cttttgaaat gaaccctgta cctgtcaata tggaacgcac tctgcaatgg ctggaaagac
     2581 aggtcatgcc gtcaatcgtg atgatagagg aaatcgacag gctgacaggt tcaaactaca
     2641 tgaaaatgat tgatgaatgt acccaccttt ctgaaaaaca ggaaatgctt gtggaacaga
     2701 tgtgtacgga catatcagac gtaatcgaaa gtgagggggt gttttatgag taatacacag
     2761 gatattcccg tatgggaaaa atacaccctt accattgaag aagcgtcaaa gtatttccgc
     2821 attggagaaa acaagttaag acgattggca gaggaaaaca aggacgctgg ctggctcatt
     2881 atgaatggca accgcataca gattaaacgc cgacagtttg aaaaggttat tgataaattg
     2941 gacgcaatct aatgcaaatg agccttgtat gtgttatgat gaacacaagt catatcaagg
     3001 ctctttccaa caaggaaagg agcagacacc atgaaagaaa aaagacggga taacaaaggt
     3061 cgtatcctgc atactggaga gagccaacga acagacggaa aatacttata taaatatgta
     3121 gacgcattgg gaaacacaaa atatgtgtat gcttggagat tgacacccac agacccgaca
     3181 ccaaagggaa aacgggaaaa accctcactt cgagaactgg aacagcagat aagacgggat
     3241 attgaggacg gtatcgacag cacaggcaag aaaatgacgc tttgccaact ctatgccaaa
     3301 cagaacgcac agaggacaaa tgtgaagaaa agcacacaga aacaacggga acaactcatg
     3361 cggttattaa aagaggacaa gttaggtgct aggagcattg atacgataaa accctctgac
     3421 gctaaagaat gggcgttacg catgaaagac aaaggctttt cctacaatac cattaacaac
     3481 cataaacgct cgttaaaagc gtcattctac atagccatac aagatgatta tgtgaggaaa
     3541 aacccttttg atttcaagct aagtgaagtc atagaaaatg ataccaaaga gaaagtcgca
     3601 ttgacagagg aacaggagca agccttattg tcatttatta agacggacaa cgtgtatcac
     3661 aagcattatg atgatgtgct gatactgtta aagacaggac ttcgtatctc ggaactgtgc
     3721 ggactgacag tagccgacat tgatttcaag aatgaagttg tgattatcga ccaccagtta
     3781 ctaaagagta aggaacaggg ctattatatt gaaacgccta agacgaaaag cgggataagg
     3841 caagtgccat taagcaaaga aacgatacag gcatttcaac gggttatcaa cagccgacca
     3901 aagataaagt cgatagaggt agacggacac agcggttttg tgtttatcaa tccgaaaggt
     3961 aatccaaaag ttgccattga ttataacgcc ttatttatcc gtatggtaaa gaaatacaac
     4021 aagcaccaca aggacaatcc cttgccacat attacaccgc atacgctacg ccatacattc
     4081 tgcacaaggc tggcaagcaa gaacatgaac ccgaaagatt tacagtatat catggggcat
     4141 tcaaacatca gtatcacaat gaactggtac gctcatgcgt ccatagatac cgcaaaatca
     4201 gaggttcagc gtctaatcgc atagaagtat ttaccacgat tttaaccacg attgacaggg
     4261 aaaatataag aagatagacc tagatatgcg aggtttacca caaaagcaaa atgcccgtag
     4321 agccgataaa ataaggcttt gcggacattt gagaagatat aaaaagatag tcaaaaagac
     4381 atatataatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     4441 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     4501 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     4561 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     4621 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     4681 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     4741 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     4801 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     4861 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     4921 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     4981 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     5041 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     5101 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     5161 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     5221 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     5281 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     5341 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     5401 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     5461 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     5521 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     5581 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     5641 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     5701 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     5761 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     5821 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     5881 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     5941 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     6001 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     6061 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     6121 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     6181 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     6241 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     6301 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     6361 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     6421 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     6481 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     6541 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     6601 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     6661 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     6721 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     6781 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     6841 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     6901 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     6961 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     7021 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     7081 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     7141 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     7201 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     7261 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     7321 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     7381 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     7441 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     7501 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     7561 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     7621 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     7681 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     7741 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     7801 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     7861 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     7921 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     7981 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     8041 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     8101 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     8161 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
     8221 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
     8281 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
     8341 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
     8401 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
     8461 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
     8521 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
     8581 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
     8641 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
     8701 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
     8761 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
     8821 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
     8881 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
     8941 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
     9001 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
     9061 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
     9121 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
     9181 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
     9241 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
     9301 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
     9361 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
     9421 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
     9481 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
     9541 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
     9601 agaacttggc tggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
     9661 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
     9721 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
     9781 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
     9841 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
     9901 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
     9961 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
    10021 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
    10081 taacgaatat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
    10141 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
    10201 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
    10261 tgatcttaga aatgatgggt caaccaaaag atgcgtacga ccatgtaaac gaccgtgcag
    10321 gacacgactt gcgttatgca atcgattcga caaaattacg ggaagagtta ggatggaagc
    10381 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
    10441 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
    10501 aatacagaga aaatgtcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
    10561 tctctttttt aaaataaaaa tcaaggagcg gataatcata tgtttttatt aactggagga
    10621 aatgggcaac taggtacaga attgcgccat ttattagatg aaaaaaatct gcaatatgtt
    10681 tcaacagatg caaaagactt ggatatcaca gatgccgaga aaacaatggc ttacatcaca
    10741 gaattaaaac ctgatgttat tttccattgt gcagcttaca cagcagtaga taaagcagaa
    10801 gaagaagcaa aagaattaga tgagaagatc aatgttgatg gtacaagaaa tgtagcactt
    10861 gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta cagactatgt tttcgatgga
    10921 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
    10981 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
    11041 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
    11101 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
    11161 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
    11221 catttgtcga acgaaaatag ctgttcttgg tatgaattcg caaaagaaat attgaaagat
    11281 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
    11341 cagtattcag tcatgagcct gaaaaagaca gaagagttag gatttgtcat accaacttgg
    11401 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
    11461 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
    11521 aagccaaaag cgaaccaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
    11581 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
    11641 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
    11701 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
    11761 tcacaagctg aagaggactt tccgttggtg aacgaggatt attcattaaa cggacaaaac
    11821 atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac
    11881 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
    11941 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag aaaaaaagct
    12001 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
    12061 caacatgtgg gcgatttgat tttctttact ttgggactga tgactgcttt ttatcattat
    12121 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
    12181 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
    12241 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
    12301 gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
    12361 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
    12421 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
    12481 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
    12541 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
    12601 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
    12661 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
    12721 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
    12781 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
    12841 ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca
    12901 gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt
    12961 tacctcgctt ttgccgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
    13021 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
    13081 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
    13141 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat
    13201 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
    13261 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
    13321 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
    13381 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
    13441 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
    13501 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatgttag tagaaaggag
    13561 agtggagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
    13621 gcttttgtta ctgacgctag tcttttttgt ttggacagca gggttaggtt ttccagcaac
    13681 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
    13741 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
    13801 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact
    13861 gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
    13921 tcgcggtatt tatctgattg tcttttcggt cttcctcgga atcacgttat atcgtgtatt
    13981 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
    14041 tatttggatc aatacgaata taatcggaag ttctttgatg actcttgctt tattgatcag
    14101 tggatttgcc agttcttttg aacgatggta tgtacgaatc atcggtgcag tagcagttgt
    14161 tgcagctttt ctcgggattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
    14221 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
    14281 tgcttacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
    14341 aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg
    14401 agaaaaatct gaacaaattt tactaggaat gaaaacattt atcttccaac gaggaaacca
    14461 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
    14521 tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
    14581 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
    14641 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
    14701 cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata
    14761 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
    14821 caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc
    14881 agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa
    14941 aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg
    15001 gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa
    15061 gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct acagttttcg
    15121 atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc
    15181 aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa
    15241 atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc
    15301 ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta
    15361 aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac
    15421 acccacttat tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct
    15481 tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga
    15541 ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg
    15601 atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat
    15661 tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt
    15721 gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg
    15781 gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat
    15841 ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata
    15901 tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc
    15961 cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa
    16021 aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga
    16081 aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct
    16141 cagctttgct agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct
    16201 atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata
    16261 tcattgaatt tgctgacatc ggagaccata tttatcaacc tgtaaaaact tattcaagcg
    16321 ggatgttcgt tcgtctagct tttgctgtag caatcaatgt gaatcccgat attttgattg
    16381 tggatgaagc attggcagta ggcgatcttg aatttcaact aaaatgtatg gaaaaattta
    16441 cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatc agttcgattc
    16501 gtcgtttttg tgatcgttcc tattggttga aaaacggaga agtcgtcgaa tatggcgata
    16561 cgatggaagt aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt aaaacagtgg
    16621 atcggaataa aaaccaagta gaacggttcg ccgctccaga tatcgtcgac gtgacgaaag
    16681 cggaattgtt ggataaagac aaaaatccgg tggatatgct ggaacaagga gaaaaactat
    16741 acgttaaagt aacttatgaa gtaaaagatg atacaatcaa aaatccggtt ttaggaattg
    16801 cgctacgaac agtcgacaat ttttatgtat gcggacttaa tactctacta gatggtatca
    16861 agattccttg gaaaaaagga gagaatatct tttatctgga gtatgaaaag ttaggtttgt
    16921 tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg
    16981 tatataagac aaagtacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg
    17041 tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg
    17101 gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa
    17161 gttttagagt ttggtcccgg aaacggccga ttaacgaaac atttaatcgg agcaaaacaa
    17221 tgtgaagtga gcattgttga gttagataag gaattgttcg attttgtcag cgaatttgct
    17281 caagatggat tttatggtga tatcgaaagt tttgagtggg caaactatta tgctggacag
    17341 acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc tggaaaaaca
    17401 ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac attccctaac
    17461 ttggcgcata attctgtcat gatcgatttg ttcaataacc aattgccgtg ggcttcttat
    17521 ggtctcctag atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt
    17581 gaaaaagcgg gattattcat taatattgaa gattatttgt acttagctgt aggtgataca
    17641 gaattaaaat caacttatga agaattgccg gaagctgtac gctatgattt caaaatgcga
    17701 ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt agcacaaagt
    17761 tccattgcag agccacaaaa ttctaattat gtaaaagtgt tagaagtaac ccagcaaacg
    17821 aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga aaatgagaca
    17881 ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc gcaacaacct
    17941 agctttatcg aattcagcgc agaagctgcc ggagaaaaac taacatttat cgattcgaat
    18001 gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt accagagttc
    18061 gttctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg ctttatcggg
    18121 gaattgacac caacaatgaa agaattgttg gaaacgattc gtcccatggc tgaagtcaca
    18181 aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct tcttgaggaa
    18241 aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac attgttggaa
    18301 agtgatgaat ggacaataaa acatagacta ggacgacgta agaaagaaac ttcgaaaaaa
    18361 atccaagaaa aagaactttc tatatgtatt gacgagaaaa tctgggatgc agaaacaaaa
    18421 atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccctt gtcctataag
    18481 ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga agaagtcaat
    18541 caagcagaac agctcgcaaa aggaacagaa gcgggatttg agttacgaat cctttgcgag
    18601 caagaacgtt cattcttagt ggaagcagta gctcaaaatg gccaatcatg gatcattgaa
    18661 atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa
    18721 ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg
    18781 gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg
    18841 tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg
    18901 ttaagaccca aagcggtttt aaggtacgtt tatcagggaa aatgagagga aaagccatct
    18961 tagacttcca aacagcgaga catcaaaatg gcatagctgt gaaattaaac ggtaaatacc
    19021 cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga
    19081 taaattatgc aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa ttggaattga
    19141 agccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacca gatttgaaag
    19201 agcaaaggaa agaagcacaa ggatttgcgt accgtccatt gatttccata cttatgcctg
    19261 tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg gatcaaacgt
    19321 atgatcattg ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt
    19381 gtctggaatc ctatcaagca aaagatgata gaatcaaagt cgttttccga aaagaaaatg
    19441 gacacatcag tctggcaact aattctgcat tggaaatagc agaaggagaa tttatagcac
    19501 tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca aaagtgctaa
    19561 acgtacatcc agaactggat gtgatctaca gtgacgaaga taaaatcgat gcagatggca
    19621 atcgctttga tccgcatttc aaagcagact ggtcgcctga tacattgatg ggaaataatt
    19681 atatctctca cttaggcgta tatcgaacaa gcattgttaa agaacttggc ggtttccgta
    19741 aaggctatga aggttctcaa gattatgatt tggtccttcg tgtgacagaa caaatccctg
    19801 cagatcatat ttaccacatt gaccgagtct tgtatcattg gcggacgatc ccaggatcaa
    19861 ctgcaagtaa tggcgaagcc aaaagttata tctatgattc cggagtgaaa gcattgacag
    19921 atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt
    19981 atgagattgc ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt ccaacgaaga
    20041 atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttattcaa
    20101 attatgaaat cattattgcg gataacggaa gtacagatcc aaaaatgcaa gaattatttg
    20161 cagaatacaa gcatcagctg aaagaccgct ttatcgtaga attgattgac attccattca
    20221 attattctcg aattaacaat ttagcagcag aaaaagcaaa cggcaaatac tttttattct
    20281 tgaataacga tacagaagtg attgagccag attggatgac ggtgatggtt tcttatgcac
    20341 agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc
    20401 atgcaggagt attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc
    20461 gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg
    20521 cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat
    20581 tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca
    20641 atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag
    20701 atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc
    20761 cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagaa ggaatcggcg
    20821 acttttcgtt gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc
    20881 acataacggc tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa
    20941 taagtcgagg ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat
    21001 tgcttttcaa atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc
    21061 gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat
    21121 tcatgctcag agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt
    21181 caaagaatcg atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc
    21241 ctctacaaaa gccactctat caattatcat gggtttaaaa taagaagtga tggagataac
    21301 cagattgttc ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat
    21361 ttcgttctca gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc
    21421 taaagcactg gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt
    21481 tcctttatac atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccga
    21541 taaacacaaa ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt
    21601 tttaggagac ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt
    21661 gacatggttt ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt
    21721 tagcttactt gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt
    21781 gaattcgccg gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc
    21841 ttcacttgaa cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt
    21901 tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc
    21961 tggaaaataa tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat
    22021 gccaatcagc aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt
    22081 tttataacca ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg
    22141 acatatattt ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata
    22201 ttacctcact ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt
    22261 cttcacgtaa tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg
    22321 aaaaatataa aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt
    22381 taatttacca acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt
    22441 cttgattaga ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct
    22501 tataaactag gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta
    22561 ggagtgtata tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat
    22621 taatggatgt tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg
    22681 gagagatccc attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga
    22741 tttttattgc gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg
    22801 acatcgtgtt tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga
    22861 cttttgcggg ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg
    22921 gtacgctttt cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc
    22981 gagataaaaa ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt
    23041 ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg
    23101 caaacatcaa aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag
    23161 aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga
    23221 atacaacatt tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa
    23281 gtattattga agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt
    23341 ttgatatcat cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag
    23401 caattttagt caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa
    23461 aaaatcaaaa ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa
    23521 aatctggacc agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta
    23581 ttcgagctgt ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt
    23641 cgatcgtagg accacgtcct gaacgtcctt tcttcgtcga tcagttcaat gcagaaaacc
    23701 cttattacta cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg
    23761 gaaaatatgt ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat
    23821 attcattgat gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa
    23881 tgtcctctca aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc
    23941 cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca
    24001 taatcgtacc agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag
    24061 aattggatta tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac
    24121 aggaaatcat tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca
    24181 atcaagggcc cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct
    24241 tattcttcga tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc
    24301 tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg
    24361 agggaagaat cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg
    24421 aagagtttat aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga
    24481 acaaatgcta tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata
    24541 gttgtgagga tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga
    24601 acccaaaagt agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc
    24661 gacctaacaa gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg
    24721 atattaataa agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt
    24781 ctatttatag tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat
    24841 tgagtgatcc agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa
    24901 aaataacaaa gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg
    24961 gaagcttcgt tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt
    25021 taatggagga aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg
    25081 gagcagagtc tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg
    25141 attttttgat ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat
    25201 taggaggccg tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag
    25261 aagtgaataa gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg
    25321 gcgggggaat taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata
    25381 gccatgcaac tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt
    25441 taaccaaatt gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg
    25501 aaagtctgtt tcaaaaccac ccctttgaga tagttcataa tggtattgat ttagagcttt
    25561 atagtaaaaa ttcagaagct aaggttaata agagaaaaga attggatatc tcaatgagta
    25621 cttttgttgt gggaaacatt ggacgattag agaaacaaaa aaatcaatct tatttgttag
    25681 agattttttt agaaatacta aaaatgaatt ctgattctta cttgatttta gtaggtgaag
    25741 ggtcacttag aaataaactg aaagccaaag cagtagaatt ggatatagag aaaagagtaa
    25801 aatttttagg agaaagaaac gatattcccg agttattatc tgtgatggat gtttttgtaa
    25861 tgccttctct ttatgaaggt ctgccaatgg ttggattaga agcacaagca gcaaaattaa
    25921 aattagtctt agcagatact atttccaaag atacaaagtt aacaaataat gtggattttg
    25981 tatcattaga tgaaacacca caagaatggg caaaaaaagt aataaaagag ccgtatgcta
    26041 ataaaataac acaagaacta aagagttatg atgtttcgta cactactgaa ca
//
LOCUS       DADVXO010000026        25548 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.027, whole genome shotgun sequence.
ACCESSION   DADVXO010000026 DADVXO010000000
VERSION     DADVXO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.027"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            172..2232
                     /locus_tag="KB950_000920"
     CDS             172..2232
                     /locus_tag="KB950_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBB6857154.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2315..2884
                     /locus_tag="KB950_000921"
     CDS             2315..2884
                     /locus_tag="KB950_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857155.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3059..3865)
                     /locus_tag="KB950_000922"
     CDS             complement(3059..3865)
                     /locus_tag="KB950_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857156.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4003..5517
                     /locus_tag="KB950_000923"
     CDS             4003..5517
                     /locus_tag="KB950_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBB6857157.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5920..6903)
                     /locus_tag="KB950_000924"
     CDS             complement(5920..6903)
                     /locus_tag="KB950_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBB6857158.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6908..7555)
                     /locus_tag="KB950_000925"
     CDS             complement(6908..7555)
                     /locus_tag="KB950_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6857159.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7640..8272)
                     /locus_tag="KB950_000926"
     CDS             complement(7640..8272)
                     /locus_tag="KB950_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6857160.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="KB950_000927"
     CDS             complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="KB950_000927"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBB6857161.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8836..9573)
                     /locus_tag="KB950_000928"
     CDS             complement(8836..9573)
                     /locus_tag="KB950_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBB6857162.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9570..10559)
                     /locus_tag="KB950_000929"
     CDS             complement(9570..10559)
                     /locus_tag="KB950_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6857163.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10817..11359
                     /locus_tag="KB950_000930"
     CDS             10817..11359
                     /locus_tag="KB950_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6857164.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11376..12461
                     /locus_tag="KB950_000931"
     CDS             11376..12461
                     /locus_tag="KB950_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857165.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12473..13279
                     /locus_tag="KB950_000932"
     CDS             12473..13279
                     /locus_tag="KB950_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857166.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13280..14107
                     /locus_tag="KB950_000933"
     CDS             13280..14107
                     /locus_tag="KB950_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857167.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14104..15177
                     /locus_tag="KB950_000934"
     CDS             14104..15177
                     /locus_tag="KB950_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6857168.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15605..18301
                     /locus_tag="KB950_000935"
     CDS             15605..18301
                     /locus_tag="KB950_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6857169.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18378..18866
                     /locus_tag="KB950_000936"
     CDS             18378..18866
                     /locus_tag="KB950_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6857170.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18878..19372
                     /locus_tag="KB950_000937"
     CDS             18878..19372
                     /locus_tag="KB950_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6857171.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19393..20202
                     /locus_tag="KB950_000938"
     CDS             19393..20202
                     /locus_tag="KB950_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6857172.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20199..21020
                     /locus_tag="KB950_000939"
     CDS             20199..21020
                     /locus_tag="KB950_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6857173.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21071..23170
                     /locus_tag="KB950_000940"
     CDS             21071..23170
                     /locus_tag="KB950_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBB6857174.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23167..24285
                     /locus_tag="KB950_000941"
     CDS             23167..24285
                     /locus_tag="KB950_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBB6857175.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24324..25202
                     /locus_tag="KB950_000942"
     CDS             24324..25202
                     /locus_tag="KB950_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6857176.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25393..25545
                     /locus_tag="KB950_000943"
     CDS             25393..25545
                     /locus_tag="KB950_000943"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBB6857177.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8074 a         4450 c         5409 g         7615 t
ORIGIN      
        1 acaaaattaa aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa
       61 aaaaatactc tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt
      121 atgaaaatta ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca
      181 aaaagacgtt caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt
      241 tctgaaaaac actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt
      301 gtctatatgc atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct
      361 gtttctgaga ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga
      421 aaacagctac ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac
      481 cactactggt taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg
      541 ctttctatct tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact
      601 gatattttga atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat
      661 gtatatgaaa aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca
      721 aaagtttcga tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat
      781 atgtccaaag taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct
      841 actatttcct tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg
      901 atccatgttc gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga
      961 gagagttacg cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga
     1021 aataaagaag aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc
     1081 gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt
     1141 tatgtagaag catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca
     1201 aataaagaac ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc
     1261 ctacctgctg aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat
     1321 gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac
     1381 tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga
     1441 ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat
     1501 cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat
     1561 tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg
     1621 gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac
     1681 ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa
     1741 cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa
     1801 atggctcatg cagtttctcc aacagggaaa gtaaaacaaa caagaagcct agagttagca
     1861 agaatatcta gaaaaatctc agaatctaat aaaacgataa ggcaagaagc tcatttggcc
     1921 ccacaagttt ctggattgga aaatgaaatt ttagagccta aaataaaaca acaatcagaa
     1981 aaagaatttg ttgctaaaca gtcggaagat cagaaggaat cagatgacca gaaggaaacg
     2041 aagaaaaaac gcttgtcaac tattgattcc accgatgagc atctgcagcc gcaagtacca
     2101 cttcctagtc cgggaacacc cgcacctggt ggaggtattc cttcttctgc tttaggagat
     2161 ttcatgcgag gtatggcagg aacatgggta tacgctaatc gaaatgaatt tgacgcagat
     2221 gactggttct gatttatatc tctatctgtt atagtcaaat aagtagctga gtcatatgtt
     2281 atttggcata atggagacga ggagggatag aaaaatgtta gataaaacgt taccttacgc
     2341 agaaatctgg atgacaagac cacttcaaca aaatgttcca atcacgcctt tagcggaagg
     2401 ttttgaattt cgattttatc aaaaagaaga tgaatctgct tgggcggaga ttgagacttc
     2461 cgttggggaa tttcaaacga cagatgaagc acttgagtat tttcaacaaa actttgctcc
     2521 ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt
     2581 agcaacctgt accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg
     2641 gttagcagtg aaatcagatt accaaggaaa agggctcgca aaagcattga ctgccagagt
     2701 cttgcaagaa tttcctaccc tccatacgga cggaccaatc tatcttcaca cacagacatg
     2761 gagccatcag gcgattgctc tttataaaaa aatgggtttt acttttattg ctgaaaatct
     2821 agacggcaca cctaatccag attatgaaaa agttatgcag gttttaagta aattagataa
     2881 ataacgaaat ggtgaaataa gtcctcagta ttgggataaa aaacgaaata gtcattcctc
     2941 atatcttact tttttgagtt aatactatcg gataactaat ggcatgccat acattcaaaa
     3001 aagaaggggc agtgacaaaa gtattgtcac tgccccttct cttgtttttt agcatgtttt
     3061 atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc
     3121 gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat
     3181 ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg
     3241 ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga
     3301 cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt
     3361 gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa
     3421 ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt
     3481 ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc
     3541 tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc
     3601 ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct
     3661 gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata
     3721 aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata ataacgacga
     3781 tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg
     3841 taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact
     3901 aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa
     3961 aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct
     4021 ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat
     4081 acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa
     4141 caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct
     4201 ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt
     4261 ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct
     4321 tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa
     4381 gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat
     4441 cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg
     4501 atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt
     4561 ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc
     4621 aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat
     4681 gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt
     4741 tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg
     4801 acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa
     4861 accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg
     4921 attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt
     4981 gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa
     5041 tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat
     5101 gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat
     5161 ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa
     5221 aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat
     5281 tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg
     5341 aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa
     5401 gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt
     5461 atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat
     5521 agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct
     5581 gattgaacac tgtttagtga agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     5641 cagccaagta aaaacagctt cttatgtttt taacttggtt ggacttatta ccgcagatgc
     5701 aagcttttga acaccgttta ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt
     5761 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag aaattccttc ggaaataagc
     5821 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     5881 aacgcttctg tcccagcctc ttttttgatc agcatgattt tattcagtta tcttcaaaat
     5941 gatttttccg atgtttttgt ttgcttccat atattcatgc gcttgtttga ctgcgctgaa
     6001 ttggaatgta cggtcaatga ttggccgaat cttgccattt tctagaaaag gcccagcttt
     6061 ttgcataaaa gtttgtgcga gctctgcttt atactgatca cttctaggtg tcaataatgt
     6121 accaagcagt tggacacgtt tcccaatcaa tacaccaagg tctatctcag gcacgatggt
     6181 cccacctagc ataccaatca agacccaacg tccatcaacg gctatagaag ctaaattctt
     6241 ttcccagtaa gaagcgccga tgaaatccaa aataacatcg actccccgac cttcagtgat
     6301 ttttcgtacc tcttcttgaa agtcttgttt tttatagttg atcaattcat ctgcaccaag
     6361 ttctttacaa aaggcgagtt tttcttctga tcctgcagta acgatgattt tcgcgtgaga
     6421 aagctgtcta gctaattgga tcgctgctgt tcccacacca cttgctcctg catggatcaa
     6481 gacagtttcg ccttcttgta attttccaag ccagtaaagt gtttgataag cagtcaaaaa
     6541 aacttctgag attccagcag cagaaacata gtctagcgta tcagggagaa ggattgcacg
     6601 atcggcaggc atcacagcat attcggcata accacctaga ttgactaatc cgtatacttt
     6661 tgtcccagga gtaaaagtgg agatatcact tttattctca actacctctc cagctacttc
     6721 gactcccaaa atagggtagg gaggtttcaa agagactgat tctcttttca taatatctgt
     6781 tcgattgaca gctgctgtat ggactttgat cagtaattcg ccttgttttg gtttaggaac
     6841 aggcacttca atttgttcta atacatctac agaaccagga tgcttgattg aaatggcacg
     6901 catggatcta ttcctccttt gcccattctt ggaatgctgt attcaactct ttgctacctt
     6961 cagaaagtac tagagtcgta cgagaaatat ccgtttcttc cactagagcg gcaagtacag
     7021 cagcaactgt agagcgagtg atcgtttttg atgccttctc attaggctta gttaattgaa
     7081 tggaagtaac ttcttcatcg ttagtcaatg tgactggttg aagaatgaca aaatctaagt
     7141 tagttcgttt cagccattca tctgcataat gtttagtaat ataataatct gtcatggact
     7201 caggccattt tgctggttca tctgcataaa cagcactgac catcagatag cgagagacat
     7261 gcgcattttc agctgcgatc actgttttga ttgcgccatc caagtctact tgcagaagat
     7321 tctttccttg agagccggca gcaaaaatca atacatcgat gcctttgaat gcttcggtca
     7381 ttttttccac ggaccaagtt aaatcaaaag agacatacga aacattctct ttttttacta
     7441 atgtttggga agcaacatct ctcacccctg caaaaatttc atgggaactt ggagctaaat
     7501 cttttatcag gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt ttcatttgga
     7561 tcatcctttc gacgtcaaat ctttccattc aatagtaaca ccaaaagaga aaaaaatcag
     7621 agatcggcta cgatgactat cagttagtaa gagaagggtg cttgaagtat cttaacaaga
     7681 cattgcatac tgctgcagtc gcaagacctg cgacgattcc ttcaggtata ccacttgtaa
     7741 aaataaccgc tagaatagct gaataaacag catttaaatt tgttccgatg actttggcat
     7801 aatcttgctg gaataaaaag tagatcatat tcatcactaa aatcgtattg gtcaatgaac
     7861 cggaaagtcc agctaagaat aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca
     7921 gacctttata aacgaaataa ggaaccaccc caatcaagat gcgaggaata aacgcgatca
     7981 gcaaggcttt ccagcttcca tgttctgttc cgatgactgg gataaacgga gaaaagacaa
     8041 aagacaaagg tgtttggata actgtgctac ggatcatgct gatcagtcca aatgtaccgc
     8101 caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca
     8161 tggttgttgc attgattgga ccgataggga taaaacctaa aggcgtaaca gctaataaaa
     8221 tcaaaatagc taaaaacatc gcagttaatg taaagttttt tgtatttttc ataagtactc
     8281 cttttttcta agcatttctt tcattaagag tcagcatgac ctgttcgatg atcgtctgaa
     8341 tatcagctaa ggcacctttt ccaaaatctc cacaagctaa aagagattct cttggttcaa
     8401 tttcctgata gccgacagat ctcaatgttt ccagattacg ctgagtgatc gggttttgat
     8461 acatattcgt gttcatggca ggggcaatca attttggtgt tcccactgga agcgccattg
     8521 cgactgttga aagaagatcg tctgcaatgc cgtttgctag cttgccgata atgtttgctg
     8581 tagcaggcgc gatgagaaac aagtcagctt gttttgctag ttcgatatga ttgattacct
     8641 gcggatttga ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga gattgcaagg
     8701 ttaatggcgt gataaaatgt gtgctgcttt tagacataat cacatctacc tgggcaccta
     8761 atttgacgaa ttgacttgta agatcagcgc ttttgtaagc agaaatactt ccagtaacgc
     8821 ctagtaagat ctttttcatt ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc
     8881 aatctcagat ttactttgag cttcttccac tgttccatct ttagataaga gatagccgtg
     8941 gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt
     9001 tatcagacta ttttttgcga ctgccagaag agattccttt ggtacatcta ctaatagttt
     9061 gaaaccaatg atttttgctt ttggctgcca ttcacgtaga taagaaatga tttttggtgt
     9121 tttcttcaaa acgagcacaa gatgctcggt gttagaagag atctttgaaa cagtggtatc
     9181 tgtctgttca ttttccgcaa accaattgtc cagttcttct tgcgttgaag aagtgggtag
     9241 gttttttgcc agctgttctt ctgaaaaact aaaagcagga gtaaaatcac tgactgccat
     9301 actgtggata atcgcatcgt agtgttcttt tttcgttagt tcttctaatt gtaaaaataa
     9361 gtccagtgca gattcgatcg tataaatcgt tatattggag tgtcttttcg gtttcagtgc
     9421 ttgtttcgta gtcacgtaat cgattgtggt atccggattg gcagctaaat aatcagctaa
     9481 tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat cgattttttc
     9541 agtcgttcct ccagctgtga ttagaatctt catttatggt cacctacatt tactagtgtt
     9601 cttccttggt gtgttccagc aagtagttta gatgctgttt caggcaattg atccagagta
     9661 atctcttgga cgaccagttc atctgcaatc tgcaaatccg ccatctgttg ccataaagaa
     9721 agacgttgat ctattggtac attgacagaa tcaattccaa tcaactggat gtttcgcaga
     9781 ataaaaggca agacagtcgc gtttagtttg attcccccag cattgccaca taaagctact
     9841 gcaccgttat aagagatcaa tggaagaaga ctgcttagct gctcgccacc tactgtgtcg
     9901 atgacatagt cgatttgctg ttttcctaaa gcttttgttg tctctggcag aaattcatca
     9961 ggtcgtacga cttgcaaagc acctagactt tttagccatg tgacagactc ttcttttctg
    10021 gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca gggctatcgc tgtactgcct
    10081 acaccgccag aagcaccagt aacgacaact tttttatcgg aggtcatacc ttgacggatt
    10141 aaagcgttca cacacaacat agcagtaaac cctgctgtac caaggatcat cgcttgacgc
    10201 agcgtcatat tttttggtag aggaactaac cagtctcctg gtactttttg atattggcta
    10261 taaccgcctg gatgggtgac gccaagccca aaaccagtag caagtacaag atcttctttt
    10321 gaaaaacgtg gattgttact ttggacgaca gttccggata aatcaatccc tggagtcatc
    10381 ggatatgttc gtatcacctg accagactcg ctcattgcca atgcatcttt ataattgaca
    10441 tcagaatagg caatttttac caagacttca ttttcagcca actcagtaaa tggcagatca
    10501 ctgattgctg tatggaaaac aggttccttt attacctgaa aagatcgaaa agcgttcatt
    10561 cgtgagacac tccttttaaa attttctttt atcataccat aaagcctact cttttattct
    10621 tgttcttccg taaatttaga caaaaattag gtgaaaaata aagaatcaaa caactagaat
    10681 ttattccaaa agataaagaa atattcagca aaaaaaattt tacaaagggt tgcctttttc
    10741 aataaatttc gtatactaat acgagtggta gtaaacagga agtttactat tagaaaactt
    10801 cgattcggag gaaattatgg aaattggtga gaagttaaga aatttgcgca tccaaaagaa
    10861 tttgacacaa gaagaactag gcgaacgcac agatttgtca aaagggtata tttcccagtt
    10921 ggaacgagat ttaagttcac catcaatgga aacattcttt tctattcttg aagtcttagg
    10981 tgtgactcca gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa
    11041 agaagatcat acggtttatt tcgatgaaga aaacgggtat gaattggaat ggctcgtggc
    11101 tgattcgaat gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg caggagaata
    11161 taagacgttt gaaccttcat tatctgaaac gtttattttt gtattagaag gggaattaga
    11221 gttaagctta ggagagacgg tctatgtggc aaaagcagga cagtcgatct attatcaagc
    11281 gacaaaacaa catcaattaa ggaatcatct caaaaaacga acaaaagtat tgattgttgc
    11341 aacgcaatca tatttgtaat atagggggta aaaacgtggg gaaaccaatc atttcatttg
    11401 atcaagtagt gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg
    11461 aacaaggaaa attttataca ctgcttggtc cttccggctg tggtaaaaca acgatcctac
    11521 gtattatagc tggattcacc gaagctacag aaggggacat ctattttgaa gggaaacggt
    11581 tgaatgattt gccagctaat aagcgacaag taaatacagt cttccaagat tatgcacttt
    11641 ttcctcatat gaatgttttt gataacgttg cttttggatt gaagatcaag aaaatgtcca
    11701 aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt cctggatacg
    11761 aaaaacggga gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag
    11821 caattgtaaa tgaaccgaaa gtattgctat tagatgaacc gttatcggcg cttgatttga
    11881 aactacgtac agatatgcag tatgaactgc gggatctgca acaacgatta ggaatcacgt
    11941 ttatttttgt tactcatgac caagaagaag cgttggcaat gagtgatgag atttttgtca
    12001 tgaataaagg gaaaatcgtc caaagtggta cacccgtaga tatttatgat gagccaatca
    12061 atcatttcgt tgctgatttt gttggggaga gcaatatcgt ggacggtgta atgatcgaag
    12121 ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa
    12181 atgaaccagt tgaagttgtt ttacgcccag aggatttgac gatcacgaca ccagataaag
    12241 gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg tgtccattat gaaatcatct
    12301 gttatgatga acagcaaaat gaatggatgg tccattcaac gaaaaaagct aaagaaggaa
    12361 gcaaagtagg attggccttt gagccagaag atatccatgt catgcgtttc aacgagtctg
    12421 aagaagaatt cgatgcacgt cttgacagct acgaagaata ggggggacaa gaatgaaaac
    12481 aatgcgcagg atctattccg tcccttatat tttgtggctc ggtttattcg tgatcgcgcc
    12541 tgtattaatg atcatttatc aatctttttt tgatatgaat gggcaattca cgctgaataa
    12601 ttatctatct tattttacgt cggggacatc gctttctatg acgatcaatt ctgtatggta
    12661 tgcatttttg atcacgtttt tcacgctgat catcagttac ccgactgctt attttttaac
    12721 aaaactgaag cacaagcaat tatggctgat gctgattatt ttgcctacat gggtgaatct
    12781 gctgcttaaa gcttacgcat ttattgggat tttcagtgtg cacggcagcg tgaatcattt
    12841 ctttgatttt atcgggatcg ggccgaaaca gatattattc acagacttca gttttctgtt
    12901 cgtggcaacg tatatcgaga ttccatttat gattatgcca attttcaatg cattggaaga
    12961 gatgaatcct tctttgatca gtgctagtag agatttaggc gcgaatagct ttgagacctt
    13021 ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt
    13081 tattccttct ctttctttat tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac
    13141 tttaggaacg gctatcgaac agcatttctt ggttacacaa aactggggga tgggttctac
    13201 aatcggtgtg attttaatca ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa
    13261 aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg tatctttatt tagggatcgt
    13321 ctttttattc ctatatgcac cgatcttcta tctgattttt tattccttca atgaaggagg
    13381 aacgatgaac agctttacag gatttacttg ggaaaattat gaagctgttt ttgaagatac
    13441 acgtttgatc attatcgtat tgaatacgtt catgctggct ttcttatctg cattgatcgc
    13501 cacgatcatc gggacatttg gggctatggg aatctattac acgaaaaaac gacgtgcacg
    13561 gacagcgttg ttgagcatga ataacatctt attagtctct cctgatgtta ttatcggtgc
    13621 tagtttcttg attttcttta cactactagg attcggatta ggttttcaga gtgtattgct
    13681 ttcccatatt gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga
    13741 aatgaacgat tcaatggtcg atgcggctag agaccttggt gctaataatt tccaagtagt
    13801 caaaaatatc attttgcctt atctttctcc aggaatcata gctggatatt ttatggcatt
    13861 tacctattct cttgatgatt ttgcggttac atttttcgtc acaggaaatg gctttacgac
    13921 gctctcagta gagatctatt ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct
    13981 aagtgcgtta gttttcttgt tctccatgat attggtcatt ggatactact ttattagtaa
    14041 agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg tggcacggct
    14101 gcgatgaaaa aattacaatc tctgttttta ggtattctca tcattattgc ggtgctgctg
    14161 cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc
    14221 tataactggg gagattatat cgatcctagt ttgatcacga aatttgaaaa agaatatgga
    14281 tacaaagtga attatgaaac atttgattct aatgaagcga tgttcacaaa aatccagcaa
    14341 ggtgggacaa actacgacat cgctatacca agtgaatata tgatccaaaa aatgatcaaa
    14401 gaaaaactag tacttccatt agatcattcg aagatcaaag gattaaataa tattgatcca
    14461 cgatttttag acttggattt tgatccagat aatcagtatt cgattccata tttttgggga
    14521 actttaggga tcgtatataa cgataaattc tttgatgcaa atcaaattaa gcattgggat
    14581 gatttgtgga agccggaatt aaaagacagt ttgatgctga ttgacggtgc acgagaagtt
    14641 atggggcttt ctttgaatag tttaggctat tctttgaata gtaagaatat ggcacagctg
    14701 acagaagcag caaataaatt gaatcattta acaccaaatg tcaaagcaat cgtggcagat
    14761 gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa
    14821 gctgccgata tgatgtggga aaatgaacat ttacattatg tgatcccacc agaaggatcc
    14881 aatctttggt ttgataatat cgttatgcca aaaaccgtga aaaataaaga aggtgcctat
    14941 gatttcatca actttatgct agaaccggaa aatgcggcac aaaatgcaga gtatattggt
    15001 tattctacac caaatgaaga agcaaaagcg ttattgccaa aagacatttc gtcggacgaa
    15061 caattctatc catctgatga cacgatctcc catcttgaag tctacaaaga tctaggatcg
    15121 aaatacctag gcatctataa tgacctattc ttagaattca agatgtatag gagataatgg
    15181 caaaactcag taagaatggg gacttatcaa cgttctaaag agctaaaaat cgtccttttt
    15241 ctatctgagt tttccaatct tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat
    15301 tttatttttt ctaccacgtt tttctaccag agaaaagtgg acaaaaccat tgtaaaatag
    15361 ggcccgagac gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaattgg
    15421 aaactatgca aatatactta acccttctgt aatctattaa taaaactaat atatgaatcc
    15481 agttcaatat ttgtagctat gaaaaaacat aaaaatcata ttagataaaa actaaattgg
    15541 tagataaatt attttgtcag atatctgagg caaatattcg actatttgtg tttcaaagta
    15601 aaaattggaa tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag
    15661 acacattaaa ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct
    15721 gatacgacta acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg
    15781 tatctctcgt cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga
    15841 gaaattaccg ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa
    15901 tacggagcaa ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga
    15961 ggtcattcaa aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat
    16021 aaatggagcc acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat
    16081 ccatgagcaa attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc
    16141 ggacaatccc gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg
    16201 ggctgaaaag gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga
    16261 tgaggtccat cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg
    16321 aaactatcgt ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt
    16381 cgccacgacg gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat
    16441 tagtgttatg ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca
    16501 attatttttt caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc
    16561 cttgtcttta atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat
    16621 taaaatcagt tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa
    16681 aaatgagatc ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc
    16741 tagtttattg ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta
    16801 tgattcgatt ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt
    16861 ttcttcgata aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt
    16921 atcaatgagt agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg
    16981 aaaaacgtac ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt
    17041 catttcccca agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct
    17101 acctcggacg acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa
    17161 ggacgagaac tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc
    17221 catcgagctg aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc
    17281 agtagtgaat caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag
    17341 taatgtgtca gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga
    17401 tagtctagtg ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa
    17461 cgatttaaaa ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat
    17521 tggcttgaag atgacgatga atgcgccatt taaacagatt gcggaggatg cagcagacaa
    17581 gtattcaatc aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc
    17641 ctgtatgtcg ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc
    17701 ggatgaatcc atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca
    17761 aaatgatgaa acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg
    17821 ttccttttca attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa
    17881 tttggagcat attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga
    17941 gctagtaaaa ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac
    18001 gatcgtgatg agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac
    18061 tttaaatggc cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt
    18121 attcttggct cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga
    18181 tgaattttat tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa
    18241 agtcaatgct tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata
    18301 aagtgtctaa aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa
    18361 ttaacgtgat taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga
    18421 acagaaagaa aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag
    18481 cttcaggctt tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga
    18541 ttaatgctta tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg
    18601 tgaacgagca ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga
    18661 agattaccaa aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc
    18721 tgccaattct gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg
    18781 aactggtaaa taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag
    18841 taaatgaaga ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga
    18901 tgaacgatta attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg
    18961 gttagtagta gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat
    19021 ggctgcacca tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt
    19081 aaaagatccg cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc
    19141 ggtaaaagtt gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg
    19201 gaaaatgaca ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac
    19261 gtcagaaatt caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc
    19321 tacaccggcg atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga
    19381 aggagaaaaa ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggagttgct
    19441 ggacatgaaa tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca
    19501 ggagcgttga tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc
    19561 tttatgggtg tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg
    19621 ctggctacag cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc
    19681 tttccactag cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga
    19741 ccgatggctg ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat
    19801 ctgcacttct ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc
    19861 gttttatttg gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg
    19921 aacggattaa cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg
    19981 atgctttgga gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat
    20041 ctcgagttac cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc
    20101 caaagagatc tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta
    20161 aatgcaacgc ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt
    20221 ctaaagatga gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt
    20281 cggtgaatcc ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta
    20341 atcatttcta caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt
    20401 tttcgacgac cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg
    20461 aaaattctga aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga
    20521 tgggaccatt ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg
    20581 cgggactagc gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag
    20641 ttatgttcaa tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat
    20701 taggctctaa atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag
    20761 ctgctagtat cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca
    20821 ataccgtttt gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg
    20881 atagtatttt taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag
    20941 ataaaaaagt cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt
    21001 cattacttgg aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag
    21061 gagggaaaag atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc
    21121 tgagttaaac gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc
    21181 tgatgtaaag ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt
    21241 ttgcgggacg attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc
    21301 aggtgtttgg gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt
    21361 aaacgggatt gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca
    21421 agactgtttg cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt
    21481 tgctcccata ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa
    21541 tgaaacacac gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta
    21601 ttcggatcag caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca
    21661 ggaagtggca agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac
    21721 ttttcaggaa tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct
    21781 atttgggcag ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta
    21841 tcaaagcttt tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg
    21901 cagctatcct gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt
    21961 caccatgttt gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt
    22021 taagtttcct ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc
    22081 cagtgcgtta ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg
    22141 gtgtccgcag gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg
    22201 gtcaggggaa ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa
    22261 gtatcatacc ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt
    22321 tgaagcccta gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa
    22381 ggaatcaatt tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg
    22441 aatcggtgat gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga
    22501 gcaagggtta atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca
    22561 tgatggagaa gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga
    22621 tcatttatat cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga
    22681 ggaaatcaaa ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac
    22741 tgttttaatg agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat
    22801 ttgtttggca gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca
    22861 aggagaagtc gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt
    22921 cagcctaatg atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga
    22981 tcctgttgat aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc
    23041 gattgaatgt acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat
    23101 aagtgatacg aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag
    23161 aaaaagatga gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc
    23221 actcccgaaa atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga
    23281 ccaagccgca aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg
    23341 gatattactg gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa
    23401 tttggcagtt ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc
    23461 cctgctgttg aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag
    23521 gtgaactcgt tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg
    23581 cctttccaaa catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca
    23641 tttttttata cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt
    23701 cacgcaaatt ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat
    23761 cgattaaagg gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt
    23821 tattggtggg gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc
    23881 gtaacctcaa caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat
    23941 gagttcggac gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa
    24001 tcaaatagag atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc
    24061 tttggtgacg acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc
    24121 tgttttaccg aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta
    24181 tttgagcggc aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga
    24241 aatactaaac gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac
    24301 atattcgaag ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat
    24361 ttgtctgtgc agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa
    24421 caactccagt tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat
    24481 tggcaggcat cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt
    24541 tcggtcatat cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc
    24601 taaagcaata tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg
    24661 gtgaatatgt tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa
    24721 caggtatcgg aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg
    24781 aaatggggca tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct
    24841 tccaccaaga ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg
    24901 taaaaggcca gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg
    24961 cccaatgtgc atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg
    25021 gtgtaatgaa acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa
    25081 atggatatgt tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata
    25141 atgccggaat aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat
    25201 aattaatgaa tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat
    25261 caaatgaagt tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg
    25321 ttgttttcat taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt
    25381 gcgctgtata ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt
    25441 tactttatgt ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat
    25501 gttgacttca taatccttgg cttaatgttt gaattttttt ggtgatag
//
LOCUS       DADVXO010000027        24549 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.028, whole genome shotgun sequence.
ACCESSION   DADVXO010000027 DADVXO010000000
VERSION     DADVXO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.028"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..638
                     /locus_tag="KB950_000944"
     CDS             <1..638
                     /locus_tag="KB950_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6857178.1"
                     /translation="DNIGISEVLLSYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEIN
                     RDSEIFSLSKDDKIVMGKRIREVRKNREETLEKFGKNFTIYTGKNVISRWEKGINIPD
                     IEKLMNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLRMRKIRKDYYL
                     EREEFGKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            1634..1885
                     /locus_tag="KB950_000945"
     CDS             1634..1885
                     /locus_tag="KB950_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857179.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            1968..2240
                     /locus_tag="KB950_000946"
     CDS             1968..2240
                     /locus_tag="KB950_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857180.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            2330..2569
                     /locus_tag="KB950_000947"
     CDS             2330..2569
                     /locus_tag="KB950_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857181.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            2716..6999
                     /locus_tag="KB950_000948"
     CDS             2716..6999
                     /locus_tag="KB950_000948"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBB6857182.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            7098..7463
                     /locus_tag="KB950_000949"
     CDS             7098..7463
                     /locus_tag="KB950_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857183.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            7465..7917
                     /locus_tag="KB950_000950"
     CDS             7465..7917
                     /locus_tag="KB950_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857184.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            8531..9841
                     /locus_tag="KB950_000951"
     CDS             8531..9841
                     /locus_tag="KB950_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBB6857185.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            9864..10175
                     /locus_tag="KB950_000952"
     CDS             9864..10175
                     /locus_tag="KB950_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857186.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            10535..11791
                     /locus_tag="KB950_000953"
     CDS             10535..11791
                     /locus_tag="KB950_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBB6857187.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            11788..12333
                     /locus_tag="KB950_000954"
     CDS             11788..12333
                     /locus_tag="KB950_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857188.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            12334..12834
                     /locus_tag="KB950_000955"
     CDS             12334..12834
                     /locus_tag="KB950_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857189.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            12915..13471
                     /locus_tag="KB950_000956"
                     /pseudo
     CDS             12915..13471
                     /locus_tag="KB950_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13491..13835
                     /locus_tag="KB950_000957"
     CDS             13491..13835
                     /locus_tag="KB950_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857190.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            13854..14765
                     /locus_tag="KB950_000958"
     CDS             13854..14765
                     /locus_tag="KB950_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6857191.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            14844..15836
                     /locus_tag="KB950_000959"
     CDS             14844..15836
                     /locus_tag="KB950_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6857192.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            15833..16051
                     /locus_tag="KB950_000960"
     CDS             15833..16051
                     /locus_tag="KB950_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857193.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            16064..16474
                     /locus_tag="KB950_000961"
     CDS             16064..16474
                     /locus_tag="KB950_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857194.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            16526..19054
                     /locus_tag="KB950_000962"
     CDS             16526..19054
                     /locus_tag="KB950_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6857195.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            19070..21280
                     /locus_tag="KB950_000963"
     CDS             19070..21280
                     /locus_tag="KB950_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857196.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            21288..22379
                     /locus_tag="KB950_000964"
     CDS             21288..22379
                     /locus_tag="KB950_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBB6857197.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            22400..22942
                     /locus_tag="KB950_000965"
     CDS             22400..22942
                     /locus_tag="KB950_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857198.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            23137..23394
                     /locus_tag="KB950_000966"
     CDS             23137..23394
                     /locus_tag="KB950_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857199.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            23408..23740
                     /locus_tag="KB950_000967"
     CDS             23408..23740
                     /locus_tag="KB950_000967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857200.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            23785..24333
                     /locus_tag="KB950_000968"
     CDS             23785..24333
                     /locus_tag="KB950_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBB6857201.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            24340..>24549
                     /locus_tag="KB950_000969"
     CDS             24340..>24549
                     /locus_tag="KB950_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857202.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEK"
BASE COUNT         8449 a         3557 c         5296 g         7247 t
ORIGIN      
        1 ctgataatat aggaattagt gaagtattac tcagttatgt gttaaataga aagagaaatc
       61 cgtcaaaaga aatgcaaaaa aaaatcgata tttttctatt aagtaatgaa aaagaaattt
      121 tgaaggagat taatcgtgat agtgagatat ttagtttatc aaaagacgat aaaattgtta
      181 tgggaaaaag aatacgagaa gtccgaaaga accgggaaga gacgcttgaa aaatttggta
      241 aaaactttac catctataca ggcaaaaatg ttataagtag atgggaaaaa ggtattaata
      301 ttccagatat tgaaaaacta atgaatattg cctatcttgg taaggtcact gttccttacc
      361 ttatgtacgg agaagattat aagaatatac ttccaaaaga tgaaagagtt agcgatttta
      421 aaaagataaa ttcttttagt atgggattaa ggatgaggaa aattaggaaa gattattatc
      481 tagaaaggga ggagtttgga aagctttttt ctccttcaat ttcaaaatgg tccatagata
      541 ggtatgagaa tggtagagat atacccaata ctaatcgaat tatccaatat gcttatatag
      601 gaaatctttc tttagaattt ttgatttacg ggatataaaa atagtttacg tcgatactta
      661 ttaagttatc gacgtttttt attatagaaa aaatttataa aaggaggttt cttgatatga
      721 taagaaacaa aaagggtttg ttgaaagttg ctttactggc agcttttttc taagtaactt
      781 tttgacaagc tcaatggcaa cacaagcgga agaagtaata caagaactaa ctgttatgaa
      841 taacagctag ataaaatggg ctatgtatgt tatccaacaa tttagtaatc actagaagcc
      901 tagcgaaagt tcgcttgata agttatagat gagtattgat ttgtagtgtt tccaaatcaa
      961 aagggtacag agccacatga aatatgttgt cctctgcagc gtcaggatgg gaatccatac
     1021 catggagatt cggagatctg tgagggaata cttacgtggt taatatgtca gttgtgttgg
     1081 acgtagtaat ggtcatagtg ggtagattga ctgaattctt atatgagaaa atgaaaccaa
     1141 caagaagata gggttattta tacagaattg gggatctttt ctggaaaagt aatcggataa
     1201 tgcgagattc gtacggtctt ttttaaattt ctctaaaaaa aacggcatcg aatgggttgg
     1261 gtgattgata agcaatgaat catagactga tatagaataa aaaatgattc tatttatctt
     1321 tttacatcta tcgttttttc aagtagccgc aaattatatc agcattcaac aatttttgta
     1381 tacaagttct agtaaaagga tctgaagaaa aagttggttg ttgtgaagat aaaaaaagaa
     1441 tcaggtttta ctgagttttt cttcaaaatt tgtgttatta ggttaggaca aagataagaa
     1501 gaagtatgaa gatttaaaaa aggaatgagg aaaagatagt aatttccgat tattggagtt
     1561 tttttattta taaagaagaa gcgaataagt tgtgattcct agatttcgcg cgtgattaat
     1621 taatgagcaa aaaatgcgga aagttttagt gtttaataca cgaggacaaa gtttcttagg
     1681 tattgaaggc acacgtgtaa cggttgatgg gtggtgtgat tgtttcgagg ttggaaaaca
     1741 tgttgaactt atggcattta ctgagctaaa agataagaac aacgtggaat ttttcgagag
     1801 tgatattggt tgggacgatc atgaagaagt ttatggaaaa tttattttgc ggaagggaaa
     1861 ttcttatacg aatggggaag cataagtagc gatttgttag agatcattga taggattgaa
     1921 attgtcggga atatttatga gaatctagag ttatttgagg tgatagaatg agtgatggtt
     1981 ttgaacaagt atcaataatt aaaaatcaag ttcgggagat tcttagaaaa aaaggctatt
     2041 tggtagatag ttattttgaa ggagattacg aaacatgggt aggtgtctat gctcgtccag
     2101 agaataaacc tacctattta gatcctacga cgtcagaaga tgcctattta caaaataggt
     2161 atcgagtaga tggttttaag caagactttg cggagtggtt tgaatgggaa attgaaaacg
     2221 gagaagtaaa agaagaataa gagtacttgt tgtatcaaaa taagccagta gaacaaccaa
     2281 accagtagct agtaatgaca ataatactga ggaatcggat cattatggta tgattgatat
     2341 ttctcttcca gaagatctta agccagatcc tgtaacaatt ggcaatagtg tcatgatttt
     2401 tgattcatta gatgaagcta tgtcatatgg ttggaaagaa attaaggatg aaggtagtaa
     2461 ttggtatggc atgagatttg gtgatggtga agttgccaaa aaagctggta ttccaattgg
     2521 gaaatggaca gttgatttcc acgaagcagg ttcttttttt gttctgtaat tatagtggga
     2581 gggtaaaatc attgatgaaa tgtgttcaga taagagcgcc agataaaaat agaagagaca
     2641 gttcacgtcg ttatcacttt tggtaacggc gttttgtttt attatataaa attctcagga
     2701 aaaggaggaa ttcttgtgta tgcaaaaacg aaaatgaatt ggttgaaagc actcgcattg
     2761 gtggcttttt ctcaattctg tttcttggtg ggaactgctc aagcacatgc agaagaaacg
     2821 acggctatac aacctacgat ggaagccatt gcccaagtcg cttctgaagt gacagaagtc
     2881 caaccagcag aagccggggt cccagctggt gaagccactg ttgaacaggc ccctgtgaca
     2941 gaagcgccaa cggtcaattc caaaacgact ctcgatcaga cgattgggga aacacaacac
     3001 ccagggaata gtcctggtac ttcacaacca gttgaagaaa ctgcctcaat tacaacccct
     3061 gcttcagaaa cgtccactct agaaggagaa aaagctgccg aagcaacgcc ttctgtgcag
     3121 ccagagtctt ctgagaaaca agcggaagaa aagaaacgaa tagaagaaac agtggaacaa
     3181 ccagcggcta ctgctgacaa agaaggaaaa aatgaggaaa acgttgaaaa aacaatctct
     3241 tcaaccgcag ctagtcaaaa tcaaccaacg aaaccaaaca aagaatctac ggctagtatt
     3301 gaaaatcaaa agaaaaaaga gcagaacatt ccaaaaacag gtacacctac actgttagta
     3361 acagtaccaa ccaatcgttc ggttcgtgtt cgccgtgcaa ccaatgcagc tgttttaggt
     3421 ggtcggaaaa ttgattgggg cgaaaatatt ggtgttactt ctagtccgaa caatctaaac
     3481 ttcactttaa attatatggt tcgttttgag attaatggac aagtggcgtt ttgtattgag
     3541 cctggtgtct cagccgcaga aggtcaagtt tatacagcta ctgctttgaa aaactatttg
     3601 aaaaattcaa atgtacggaa aaaaacaagc ttgatctctt atttaggata tgtcacaaat
     3661 aaagacaaat ccgatgaaca atacattgcg gcacaattta tgatttggga agctttgggc
     3721 acaaaaatcc caatcaaaaa tatccgtgtt gattatgatc gtcgaaaagc agaaattcaa
     3781 cgtttgatta ataactttgg tgaaaaagca acttttaata atcaaacaca tacggtaaaa
     3841 attggcgaaa cgttgaaagt aaagaataaa aatgattttt tgaaatatgt gaaagacatt
     3901 caaaaaccaa aaggtgtcaa tgtatctatt cacggcaatg aagtatggat tacagtcaca
     3961 aaagaggcac cggataaagc aaaaattcaa ttgaataaaa ttaaagttca aggtgcgcca
     4021 ttagcctatc gtcgtccagg agctcaaaca attggtgtgt tacatccaac agaacctaat
     4081 gcgtctgtta ttaatttaca aattctaaaa caaggaaaca ttcgtgtgtt taaagaagat
     4141 gcggaaactg gtgtgaaagc acaaggtgca gcttctttag aaaatgcggt ctatggtttg
     4201 tatcaagcag atgggaaaaa gattaaagaa attaccttga aaaaaattaa cggcaaagta
     4261 caggcagaaa ttaaagatct tgatcctggc aaatatgttt taaaagaaat caaagcgcca
     4321 aaaggttatg tgctttctga tgaagcaatt gaaattgttg ttgaacctgg aaaaactgtc
     4381 aatgcgaccg caaaagatca agtgatcaaa ggaaccattg atttagtgaa agttgctaat
     4441 aaagatttag tcgattcaac gaatccagat aacaaaccaa aattagctgg cattgaaatc
     4501 actttgacgt caaaaacaac tggcaaagtg gtgaaaaccg ttgtgacaga caatgatggt
     4561 tatgctactt ttggtaaaaa taccgttgtc tttgatacgt acattctttc tgaaaccaaa
     4621 ggaaaagaag ggtataaact atttgaacca cttgaagtca caatttctga aaatgggcaa
     4681 actttccatt atgttttaga agataaagta attgaacaac gtcttaaagt ggtgaaagtg
     4741 gatcaagaaa caggaaaagt gattccactt gctaatacag aattcaagat ttttgatact
     4801 ttagccaatc gttatgtcac aatggagatt ccaaatgata cagaaacaac ggatatcttt
     4861 aaaaccaatg aaaaaggctt tttcctaact aacggtacac ttacttatgg aaaagatcgc
     4921 tatcgtttgg aagaaatcaa agcccccgta aattacgttt tgaatcaaaa cccattagtg
     4981 ttctcgatta ccaatgatac agaaccgatc aaagtgatcc attttgcgaa ccgtcaagca
     5041 cggaaaagtg taaaattatt caagtatgaa gaatggaaca agaagaaaac gccgctcgca
     5101 ggtgtgaact ttaccttgta taacggtaaa aatcaagcaa ttggtgaata taccactgat
     5161 caaaacgggg aaattttagt gaaaggtcta gttgccgatg actattactt cttggaaaat
     5221 gccccattag aaagtttcca accaaataca gataaacaag cattcacggt tgcattcaaa
     5281 caaaatgcgc cagatgatgg ggaaacgtta gtggtttctg tacgtaatta cttgattcca
     5341 ccaaaactag aaacacaagc gtcaaataaa gccgatggtg aaaaagtggt tgatccatta
     5401 gaaacgatcg tcattaaaga cagtgtttct tataccaact tgtttgtcgg aaaagaatac
     5461 accatttcag gagtattgat gaacaaggca acgaatgcgc caattttaca agatggcaaa
     5521 gaaatcacaa gtaccttggc ctttattgca gacaaagtca atggcacaaa agaccttgaa
     5581 tttgtggtta atgctagtgt tctgaaaggt caaacgatcg ttgtttttga aaaacttttc
     5641 cgtgatggca ttgaagttgc agcacatgcg gatatcaacg atgaagccca aagtgtgaaa
     5701 gtgaataatc caaccgttca taccacggct gtctttgaag acggattgac agtcgcagac
     5761 ccaacaggat tgtttacctt aaacgatacc gttgaaatta ccgagattct tccaggaaaa
     5821 gcatacttgg tcaaagggat cttaatggat aaagcaaccg gtgaaccctt gcttgtgcat
     5881 ggaaatacgg tagaaacgag tgttggcttt attgccaaag cagaaaatga aacggtgatc
     5941 gtaccattta cctttgattt gacaggtata aatggtcgtg aaatcgtcgt gtttgaaagt
     6001 ctttttagaa atgatgaaga actagcaaac cataaagaca tcaacgacaa agggcaaacg
     6061 gtacgagtaa ctaacccaag tgtacgtaca acggctacac ataaacccac tggcttgaaa
     6121 gaggttaatc cattaacgaa agtcaccatt acagacgctg tgaaatacca cgatttggtc
     6181 gttggaaaaa catacacagt caacggtgtt ctaatggaca aagtaacgga aaaaccagtg
     6241 ctagtagatg gaaaagaagt cacaagtacc ttaacgtttg tggcagaaac aacggatggt
     6301 acagtcaatc tagactttat tctagacgca cgtgcgttgc gaggcaaaga agtcgtggtc
     6361 tttgaagatc tgtttagaga aggcgtatta ttggcttctc acgcagatat taccgacaat
     6421 gaccaaacca tcaaaatcag taatccaacc attacgacga aagcgacgaa taaaaatggc
     6481 ggaaaagaga tcttggcctt gccaaatcaa actgtgcttg aatgggtcaa agtagacggt
     6541 cttgcgattg gtcaaatcta tcgcttaatt gttcaaggct acttaacacc agacggcaca
     6601 ccatttgaag gcactcatgc ggaaaaagtc tttgcggccg ataaagaaat gatggaattc
     6661 ttatttgaat tcttagtgga cggtcgcaat cttgcaggaa aaggcttgag ttttgcggaa
     6721 tggctccaag cgaaaacgga agatgagaaa gaaacgtttg aagaagtggc gaaacacaat
     6781 gtagatctaa caaacaaaga tcaaacggtt caaattgtgg aagcgccaaa accaccgcaa
     6841 ccagtgaaac cagaacaacg ggttaaacca gctactgttg taccaatgtt gccaaaaaca
     6901 ggtgaacaaa caagtagtat acttatggtt cttggattag tagtattagt cattgcttta
     6961 ggtataacac tttataaagt acgtaaagcc aaagattagt catgaggaac aagcggttag
     7021 gatttttaac cgcttgtttg attgtaatgt agaaatattt agaatttatt ttggttttta
     7081 agaggaggaa aaactttatg gcagaaaaag cattgcgtat gaagaaagag tttttaggtc
     7141 aagtattttt attgggttca cgggaaccta atccaccaaa ggaaattaat ggtgtgtctg
     7201 ggcgatatgt tcaattacaa gcagagtgtt cgcgagaaga acagcctgct ttttttgttt
     7261 atctcacagc tgaagaggat aaaagattac gattaccaga aaaagaggtg cgtatttcat
     7321 ttgaagaagc ttattttgtt tttcgtaaag aacgagtgaa tagtcgtgaa acaaatgata
     7381 tgggacaaac agagcgtcga acaactggct atcaagatgt tggtgtcttt ttaactggtg
     7441 taaaaattgg ggaggttttg taagatggga ttaaaatttc caaataatta tgacattaca
     7501 gaagcattag atgaaacttg tttaggagcc gtacgctttg gcgcattaga agagtctgat
     7561 cgtgtatttg ataatgaaac aggcgattat acgggagaaa ttcgatcaca gcgagcacaa
     7621 ttagtttttg gaaatccaga tgaaccaaag attgcacgac gagttaaatt tccagcagat
     7681 gtagatttga ctggttttaa acgacgtgat cctgtgaaat taattgatcc agaatatcat
     7741 gtggtatata ttccagcaca agatggcaat cgtgaatatt cagatttagt gatttatgcg
     7801 aaaggattag agaaagtaga agctccaaca aataatccta atatgaaaca atcacaagaa
     7861 aaaggtttag atcaaaaaca accaagtgtt gattctaagg aaaaagagaa aaaataaggt
     7921 ttttttgaaa gaataggaag gagtcttcct attcttttta tctaagatag aaggaagggg
     7981 agattttgaa ttcagataaa accaatcaaa tactttgaga gattgtgctt gttatcaaaa
     8041 tatccaacaa ttgattgagt attttggaga caatccaacg atttttgtac aaacaaatgc
     8101 atatatttgt gcggttattg ggcagtgtat gtttttacgt gatcttgttc gtttttcatt
     8161 accgtaccag tggaaacaag atcatcagga agtgatttgg attcgtatag tacgaatatg
     8221 taatatgtgt gcacaccctt ataaaaatcg attaaatctt tttcgattat gtttttttac
     8281 taaatgtgat attcctgtat tagttgcttt ttgtcaaaag tttttagcac aggaaaagta
     8341 aaaacagaaa ggaggaatgt gacaattgaa agctcatttt ttcaagtatc gtggtcggcg
     8401 aattcgctat ttcaatcgtt atttatggtt ttgctattat ggtttctttt tgtttccatt
     8461 tttgattctg ttaggttggt ttagttggaa agtattttat ccacgtttaa gtacgttttc
     8521 gaattggcaa atgtatctta ttcctggaat aagtattgtg ttgtatttgt tgctggttag
     8581 tgggttaatt ttagggttga tgcattggtc gcgactaaaa gaaggatatt ttgcctctgt
     8641 ttattggcgt cagttacttg ttcgaatgtt aattgacaat cggtttgttt atacaaagaa
     8701 acaagttagt gagaaacaaa caagggaaaa aatgagatta ccaaaagttt atttttatca
     8761 aaagaaacaa gaattagtgg tttcttttcc tttagatggt gggcgttttc acgaacgatt
     8821 tttaaaaatg ggtcatttgt tatctgaagt atttttgcgt gatttaattc gtgaagaacg
     8881 agaaaaagcc cgaatcaatt atgtattcct ttcagattct ggtcggattc ctattgatca
     8941 atgtatagca gaaaatggac gtattcattt aatgaaaact ttggattggg tatatgatca
     9001 atcaccaaat atgttgattg ctggtgcaat cggtggtggg aaaacctatt tattgtattc
     9061 tttaattcaa gcttgtttaa gtgttgggac agtagatatt tgcgatggaa aagcggcaga
     9121 tttagcagca ttaggtgacg tcgatgtatt caaaggacat gtgttttaca aaataaatga
     9181 agaaatgatt atttgtttac gaaatgcatt aaaagaaatg aatcaacggt atgcctatat
     9241 gaaaaccatg aaagatccta aatatgaacc agggaaaaat tatgcttatt atgggttacc
     9301 accgcatttt atcttttttg atgaatgggc agctttttat ggttcgttgg atatggaagc
     9361 gagaaataga gtcgataaat tgattcagcc attagtatta aagggtcgtc aagcagggat
     9421 gtacattatt ttagcaatgc aacgaccaga tgcagaatat tttccaagtg gtgttcggga
     9481 taatttaaca ttccgtattt ctgttggtcg attgtctccc attggttatt taatgacttt
     9541 tggagaagat tttaaaaata agccatttta caacaaagat caacctggtc gtggatatgc
     9601 ttttccagga tttgatgtac ctgtagagtt ttttgcgcca tttgtttaca aagattatca
     9661 ctttatcaaa gaatttgaga aatttactga aatgattgtc tatgatttta cagtaaaaga
     9721 ggatagtcaa gatcaaacga tgggagatga taatcagttg ttacaagaac aactagagcg
     9781 ccaaatgtat ttagcaaaag atccaaaagg aacaagtttt tctgatgcgc atattttgta
     9841 atcaactaag gaggtgaagt taaatggcag agagttgtta ttatagagta gctacagcaa
     9901 tacgaatgat taatccttct ctttcttcca gaacgtttta tgattggttg aatcgtattg
     9961 aacaagtaac tgattaccgc tttttgcgca aagaacgtgt ttttacagga aaagttatta
    10021 atcaagtatt gctaacgaaa aaagatatag aacgattgac tcgattgtat cattatcgag
    10081 tagatttaga agaggattta actttgtcaa tttatcgcgt tttttctcct gaaaaatata
    10141 gtgaaataac aaaattagat catttaattt tgtagttatt atctatcggg agggctctag
    10201 acgtagtctg gtagccgcac tctgcctggc ggtgcgcatt aaggctgcga agcatgggcc
    10261 tataatgcgc actcgctagc agcgtgtgca gctcacaaag aggtcgtgcg cttgcgcacg
    10321 gcctctttgt gtaaagcccc ccgatatata atatgggggg cagaaataac ataacacata
    10381 gaaaaaagtt ttataaaccc ttttgtatca agtgattatc taaggtcagc agttgtacta
    10441 cttttcccaa aaaacgaggg ggactagttg atatatcggt gtttctaaag ggttaaaaaa
    10501 atcgattttg ttattttata aaaggagcga gaaaatgaca ccgattgagc taaaaagcta
    10561 cagaaagaag ttaagtgcaa ttcataatat aagtggagat aaaaagccgc tatcacaagc
    10621 aagatttgcg caattgatgg gctgctcaaa agattacatt agaaagatgg aacaagggaa
    10681 aagaaaaatc ccagaggatt ttgaacaacg aatcaaggaa cgctttcgat tgtatatgaa
    10741 aagcggaata gatcttcgag caatgattga ttatttgcgt ctttcctttt ttgggacaga
    10801 ctacaaaaca gtgattcaac gtgtattacg aatggatctg acagaatttg aaacacgtcc
    10861 aacgggcttg tatctttttg actgtctttc tgttcgtggg aatatctggg tgttctggca
    10921 ttcaaaacga actgtaacag aaaatgtact tgtgcaattt tcaggacaag gttgccggga
    10981 atatgaggaa gtcatgcgtg aacaacaaac agattggcga acatggctat attccatttg
    11041 gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg caatgtagcc gattcgatat
    11101 tgcgttagat gaactttggg gattggaaac ggaagaacat ttcgatctct ttgaattact
    11161 agaaaaacaa aaagcaggtt tattagaaat ggactttaag agattccaga atattggcgg
    11221 ttgtttttta gatgatggct attttcgaag tgaaggatta tctttgtatt ttggatcgcg
    11281 aaagagtcct ctctttttta atttttatga gaaacgtttt gaaattgcga accgagaaaa
    11341 aatttcagaa ttagaagcat taacgaaata tggtatttat aatcgatatg agttacggtt
    11401 agccaatgag aaagcaacac aagcagtaga agcatttatt ttaggtgatt caccgaaacg
    11461 tttaggagag attggcgttg gtttaattaa tgcaaaactt attgtttggg atagactgga
    11521 aagtgatgca cggattgttg ataaacgatg ggcaaaatta tttcgttcga tggaagaatt
    11581 ggattttaca gtaaagccag aaaaattctc attagatcgt tcagaacgct ggtttttgaa
    11641 acaagtagct ccgacattaa agttgttaca agaagcagat cgtattttag gaagagactt
    11701 tgtacaagaa gggattgata atgccgaact aaatgacgag caacaaaact atctagaatg
    11761 gatgggaacg tcaggaggga aaattgcatg aatgcatata cgacaacaga atggctgcgc
    11821 ctgttggatt taaaggctgc aatcgaagca ttaaatgaaa agatggttga tttgtcttat
    11881 tttcgttttc gagttcctta tattgaacaa gcggtaaaag ctggacgtta tcaggaaaaa
    11941 gagaactggc aagaaattgc acgcttatta gaagttcgta agggatacga acaagaactc
    12001 gaagagttgg aatttagccg aaggaaagga aggctagaat tcattcgctt ttatcgattt
    12061 tcattgccaa tacctgcgat tttagcggtt aaaaaaggct gcgataaaat gaagatatat
    12121 gaaaactgtg tggcagcact tagcagcgaa aaacctctta tggaagaaat ttcattggtg
    12181 actgtcactt gggaaatgag tcgccagcca acagaagaac agacatatct ttctttagaa
    12241 gagattgaga tagaattaga ggaaattggt cgctatgcca cttgttctac ctattgtgga
    12301 tcggtcatta gtattgctgg tgtgattgtc taaatgaaaa aaattatgat ttatgttggt
    12361 gcgtattggt cacgtgatcc aacagtatta gaaaatccag aggcggtaca ctattgcttg
    12421 cgccaattat tctatctgta taaagaacga ttagaatcgt tgattcgtca gttaccatat
    12481 actgacagac gtttagatga acttcttcta aggtatccag caatgtataa acgacgaaaa
    12541 aatagattgt taccagaaga gtatcccatt gaaaaacatg aacttgaagg gcgatttgtg
    12601 gcttatttct atgatgatgt tcgtatgcgc ttagtagaac aacgaatgga aatagaaaat
    12661 gatcgctact attttatcaa ttattgtaag aagcgtaagt atcaagtaac tgatgatttt
    12721 tatgattgta ttttacaaga tggggaagtg attctatcca aaattgctcc aagttttcgt
    12781 gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc ggattcttcc gtaaaaaaga
    12841 gctataaaag gaaagaaaag tagaagccaa atcggtttct gcttttttat tttttttagg
    12901 aaggagaaga tggtatgtca ctggaatttt taggagagcc aattgaaaag gaagagttcg
    12961 tttcgcatta tatggatttg tttattcagg tgataaaaaa ctttgtgcga ttgatgaatt
    13021 ttatccacgc gaacaagact atctagccgc tgaacgaata aaaatcagtt tattatggga
    13081 aaaattcaca gatgaaaata accgaccgcc agattatcgc tatttatcag attgtttaac
    13141 gaataatgtg ctttctaaag aatacctatt tcacaaacgt gagaaaaatc aaatgtcggt
    13201 tgaacatttt tgtgattgtt atattgcatt attgcgtagt caacagttat taacagtaga
    13261 acgatatggt gcagagtttg gttatgtctt gcaagctgag cgtcatagag ctacaagatg
    13321 ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa ttatgtattc gaaacaatcg
    13381 ctatttgcaa caacgtttat atgaacaatt gatgatggat ttttcaaatc tctatagagg
    13441 gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg gggaattttt atgtggcgat
    13501 tattattttt ggtggtatgt ggtatggcat tgttctatgt gtatcgtgtc tatctctatc
    13561 cacgaatgga acgatggaca agttggtata gagatccttt aaaacaacga aaaggattga
    13621 agcggtatcg ttatgaatta cttgttgaat tttcggatgg gcgacaagca cagtattatt
    13681 tttttgcaaa ctatccaaaa tacaacacca gtgaactatt gattcaatta ttcaaaaata
    13741 atccatatgt tgggttagaa gaaaacggac gggagttctt ttatcgttca gataatatta
    13801 gtgcggcgtc tatgaacaaa acacgaattg cataaaaaat tggaggaata atcatgaaaa
    13861 aaacaacatt agcagtcgta acaacactag gaatttcatt attttcgttt caattaggga
    13921 gttcattggt ttatgcagat gaaactgtag aaacacctaa gtcagaaatt gtagaaacat
    13981 tgccagggga aggattacca gatgttgaga cagagctaaa tggaggaaca acggaaaaac
    14041 cagcagaacc tgaacaattg cagtcagaga aacctgaaaa agagccagtg acaccagaac
    14101 cagagtttcc tgaatcaact ttaccgactg attcgatcga agaagaaggt gtgcatagtt
    14161 caacaacaac ggattcaagt atagtcacaa ctccggaaca atcaggaaca accagttctt
    14221 ctgaaaagaa tactgattcg tcaagtacgg aacagccaac gatgtcgtct accagtgaag
    14281 aacaaaaaga agaaccgaaa caacctgttg aaaaatcaga agcaattcca aaagaaaata
    14341 aacctattgt tccagcaaaa gaagtcacag tatcggtaac cccaagcggt gaaattacga
    14401 cgaatacttc tcaaggaatg agtgtaccga ttgtcactag taatgtagag gaattgaccc
    14461 atattccaac accaaccacc ccattaaaag tagcaggtga tcaaacaatt gttggtgtaa
    14521 aagatggtat tccattaatc caagatagtg aagggaattt ggtaaaggat ttgtcgattc
    14581 ctgtaaagaa acttccaagt ggcaatattg aaatcaaaac agcagatgga aaaacaaaag
    14641 tcttaccaaa gactggtgaa gaaatacatg ttgcactttc tgttctagga ggagtgttga
    14701 caagtattgc tggatttgtt ggatataaaa gaaagagaaa aggaatagaa aatgagggta
    14761 attgaatgga ctatttttca gaaaaacaag cttatcgttg tcaatcgtac gtactagtaa
    14821 aaacatacta gaaggagtag cttatgaaaa aacgacaact acggcggaaa gaaaaagttt
    14881 atacagagaa agtaccgaag caaaagaaaa tccattcaaa aaaagcagtg aaatggattt
    14941 ggtgtttggt tttatttctt tgtttttcag ggggcttggc attttttcaa tccatgaaaa
    15001 cgaaggttca agtgtcgttt ttgaaagatg aaatccaaca attacaaaag aaagaagtac
    15061 aaagtcggtc agaattaacg tacacacctg aaattgaatc ctttatgaat cgttttgttg
    15121 ctttgtatat gaatacattc gataatcatg aacaagaaca acaacgtcaa gaaacattaa
    15181 aaaaagagtt ttatgcacaa aactttattc aagaaccagg aagtgtttca ggtgttcgaa
    15241 aattaactgc tgcttcgttt attggattaa aagaaattga aaccattcca actgctagtt
    15301 ataaagtaac ttatgatatt cgctcgatga atccagagag taagaaagaa acaagtgtga
    15361 caatcacaca agtactcaat cttccctttt ctatttcaaa caagggctgt cgtattattg
    15421 cttatcctta ttttacggca ctaccaaaga atacagaaaa acaacgaatg attacttatg
    15481 atgacacatt atacgaaacg gttgatgcgt cggaagcgaa agatgttgaa ggatttgtgg
    15541 aagatttctt agataagtat gcgaaaaaca atgcaaaaga tatgtcatac atgatgaaag
    15601 aacctgaagg actcaatcaa acagctaaag ttgatcaagt agagatgaaa gtattgcgtt
    15661 ataaagaaac gaaaaaacct tttattgtaa aagcaacggt cgtctttcga gatggtgata
    15721 cgtcactttt acaaaaagaa caaatgacga ttgtcattac gaaaaaaaca aatcagtatt
    15781 atgttgaaaa attaacacat aattggttgg ataggaaagg agaagaacgg aaatgacact
    15841 gaagggaatt gtagatttta tattaacgca aggtggctat tttttaataa tggtggttgt
    15901 ttatctggga ttgcgaaatt ttatgaaatc aaaagtagga aatgcattaa tatctatcgc
    15961 agcaggtgcc gcagtttatt tcttcttaaa tgatccaaca cgtgtatttg atgcgtttgg
    16021 taaaatcatt gaaaaattat ttatggatta ggaggatagt gaagtggatt gggaacctta
    16081 tgattattcg attgtttatg cggaacctta tcgcgtacaa cagattacaa gcaattggcg
    16141 tcttccattt ccagttgcat tagtcaatgc agcaactttt ttgttggtcg taatctttga
    16201 atatttattt ttacgttcat ttattggttg ggtcatttct ttttttccac cagcaggagt
    16261 tgttttttat ataggactcc cctatttatg tgtgttagca ctggcaaaac taaaacctga
    16321 tggaaaaaaa gtgcattttt atttatggga ttggttttgt tatatgattc gtatgatgaa
    16381 tggtgattgt gtgtattata actgggacaa ggtatcaaac aatgcattgg aagaattact
    16441 ttttgtgaat aagtatgttt atttagagga ttagaaaaag ggacaatcaa ctggttgttc
    16501 cttttttatt gggaggtgaa attcattggt gcttgtaaaa gaaccagtaa aagcattcaa
    16561 gaaaaattgt ttgttaaatg gagaggattg tatctgggcc tactatcatg tttttccacg
    16621 aacaatttca aaaaacacac caaaaaagac acaggaaata aaagaaatga tggctcgttt
    16681 actgttgctt ttagcagatt atgaagatgt ggagttggtg atgtatccac gaacaatgaa
    16741 tttacggacg aagtttgaca cattaggaaa gggattttct cccgatagtc aagcaattgg
    16801 tgcgtattat gcaaaagaaa cagtgacatt gttggaacga gaaggtcaag tctatcgtcc
    16861 ttcatttatt ttaggaatca aagtcaaaga aaaagcaacg gtggagagtt ttcatgatgt
    16921 aataaatcta atctggttag atttagcagg aagtttagca cgcttatttg gattagaagt
    16981 agatgatttg gaaactttgt tttctgaaag tgatccttat gtaaaagaag tgacacaatt
    17041 actttatagc ttttctattc gtcccttagt aaaaaaagag ctgttgtatc tcaatcgtct
    17101 gcaatatatt cgaggaatgg ctcacaaagt agaagaacaa aataattggg aagtactgaa
    17161 agacaatcaa ggaacgtatg tacgtatacc gaaggaacat aaaggccttc ttcaattaga
    17221 agatgagtta gggagtagtt atttagcatt gttgcctatt ggggaaacac ctgaaaatat
    17281 gagaaattgt gatctatttc aatttgctca ggatctttct ttcccagcag aattgcgaat
    17341 gaaaattcac tatccacgct tgaaaggaca gggaggtatt ggtttaaaac tgaattggct
    17401 atcggaacgg ttcaaaacag agcataagga attggttcaa aatgatgaag aacctagtga
    17461 acgcttatta actgttcgag gattagtaaa acatttaaag caacgagtag ataatgggca
    17521 acctgtcgtc gattggatcg catgttttat tgtatacggt caaacggttg aagaagttcg
    17581 tcaaaatagt gcaacggtta ttcaacaact tcatacgatt atccatatcc accgtgcaaa
    17641 aaatgatcaa cgagaattgt tctataaatg tttattaggg caaccattgg gacggcagtt
    17701 acattggaag caccgaacaa ctgtagaagc agttggcgaa tggttgtttt cagcagtatc
    17761 agaattaggt atggaatccg gctggtatat tggtcggcaa gatacattag gggatggtgg
    17821 ttctcgagga gaaaaaacac cattagaaga attaatttat gcgtcaaatc gttttgtctt
    17881 tttgaatctt ctagccattg cggaaggaat caaaggtgcg ttgtttgatg cgcctcatgt
    17941 agcagtaact ggaaaaaccg gtaaagggaa gaccttttta gttggcttgc tctttttgta
    18001 tagttcgttt atgaatgtac aaagtttatt tgtggatccc aaaggagaaa agcgcaaatg
    18061 gtttacgaaa ctcattcaag acccgtatta tcagaaacat taccctttgt ttgtagaaca
    18121 tttgcaaaaa atgagttatg tcacgttaga tgctacaaat ccccgtaatt atggtgtatt
    18181 agatccaatt gtttgtttat cccgagtaga tgcgaaacaa gtagcccagg atatgattga
    18241 tgagttaagt ccgttaggta ccaatcacct tttaaaaggg gcggtattaa agggaatcca
    18301 agaagtttta gttcaaaaag aagccggaca acaagttggc ttgatgcatg tggtcgacta
    18361 tttagaacaa atggaacaaa aagaggttcg agaatatggt gagtttttac gcttgacggt
    18421 agaagattcg attttacgtt taggattttc ttatggagaa aacccgggat tagactttaa
    18481 tgccaaaaca acgattttag agatccaaga tttgaaatta ccaaatgaca atgtccgacc
    18541 agaactatat acagatgcgg atcgaaagtc tttgtgtttg atgattagtt taggtcgttt
    18601 ttgtgaaatg tttggaaaac gagatagtag taagaaaaca gcgatttatt ttacagaagc
    18661 atgggtattt aataattcga acgcaggacg tagtattatt gcagcaatgg cacgtgtggg
    18721 gcgttcccag atgaatcaat tagtgttaga tacacaattt attggtgact taggttcaga
    18781 taaagaaaaa gggaattttg gtgtcgtctt tgcttttgat gaagacagtg agcgagaaaa
    18841 gattttgaat catttacgtt tggaagtaaa tgaagcgaac ttagctgaaa tgagccacat
    18901 gattaaagga cagtgttgga tgttagatcc ctatggacgt gtaggaaaac tcaatgtaca
    18961 cagtttgttt gaagaaatga ccgctgcatt acaaacaacg gaaaaaacaa gtcactctca
    19021 agtagaagaa caggatacgt taatcatgca atagaaatga ggtgaaacaa tggcttatat
    19081 aaagcataag gaaaagaaat ggtgcgtttt gtttttttta acgattggat tcggactgct
    19141 ttttgttggt ggattggtgt atgcggcaga aaagcaggtg gatcaatcga tctatgagat
    19201 tgggaaattt catattgata ccttttcttc ttatttatct gatagtgaat tttcattagg
    19261 caaaatgtta tttggggcaa atggcacaga ttttctaaaa atgatgacga atatgttctt
    19321 tagtttaagt aaattgattt ggcaaggcct tgattttgtc attgagaagc tctatgaagg
    19381 agctgcaatg aacgagttaa ttcaccagtt tttcactttt tcggatacta tttattcaaa
    19441 aatatacgat cctattggtg cggggattgt cgtcttgtat gtgttatatc tatttactct
    19501 tcgtatattt agagggaaac attacgcaaa aatggtgttt attcgatttc tcttcgttgt
    19561 cattttttca atggtttggt ttggctatgg tagtaacgta cctagtaaag gagaagcttt
    19621 taccaaaggg ttaaatgatt tgtccgtgga ggtagaaggc tttatttttt ctgctacaaa
    19681 tagtctagat gaattggcga caaaagatgt caatggtgtt ttagtgccaa cgacacaaaa
    19741 ggaagccatt caaaaagtgc gccaacttta ttataaagcg gctgttgttg atccgtatat
    19801 attgttaaat tatgggacaa caaatttaga agaattgtat caagcagaga ttgaaccaca
    19861 agaatttcta gccaaggaag cgtcaagtga agaattatct gatattgaga caaaagtgac
    19921 cactgctgca ggagaagata gagggaaaaa gaaggcttat cgtgtctata ttcaaccaac
    19981 taaagctgtt tataaagcag tcgttggttc tatggtgcca ttgcttaatc taagtattgg
    20041 attaccactt gtagtgattg gcatgattcg ttttatcttt caaatgttgg tgttgtgttt
    20101 attacttgtg attccttttt cattactcgc ttcttttgtt ccagtatttg attatatgct
    20161 catgaatacc ttaaagtcct ttttgggctt gatgtttcaa aaatcttttt acagtgtgat
    20221 tattttggtg gcatttttga tctataacat agtagacgat ttaattccaa tgaatactgt
    20281 ttttggtttt ttaggaaata tgtttgtcaa agcactccta tcaattttac tcattgtaaa
    20341 acggaaagat ttattaaaga aactagggtt aggagctgca gatcaaacta taaacaacac
    20401 gaaaaacata gcaagtcatt ctttgtctca aatgaaacaa ctgcctggtc aactatcaac
    20461 tgctggacaa aatttggcaa tcaaaggtgc aggattagca gggaatgtgt atcgagcaac
    20521 gaatccactc gcttctgcag cgatacatgg atttcaaaag tttcaacaaa aacgtacacc
    20581 acaagaatcg gtaaaaggga ggcaagtggt taatcccttt gaactaggtg ggcgttcaaa
    20641 tcaagccttg ccttatagtg gtcgtcatgt tcgttatcat ggggtagagc ctgaaatgtc
    20701 ttctaccaaa tcgaaaacag agtggaattc tggtctttca aatggtttga ttccaaattc
    20761 gtccatgaat tcagtaagac caaaacaaca ggtacaatca ttggcacaat tgagtaagca
    20821 acaagggaat aatcaaagaa aaccattgtt aaataaaggt atcgcttttg ttggtcaatc
    20881 gattccgttg ccttctggaa aagtacccgt gccacaatta acagctgctg gttctctttc
    20941 acaacagatt cgccaagtga aaaatcaggc gaaaaatggg agttatccag caatgcagca
    21001 gttattgaac caatactctg ttcctaattc gtcggtagcc actctatcag gaaatcgttc
    21061 tgtgcaacaa ccggggcaac gttcgtctga aagtaaaccg tcgatttccg caccaaaatc
    21121 gttacaacct gcattttctt ctttagataa gccaacagag caatcggaaa aaatagaaaa
    21181 ggatcctaaa acactgccga ttttggacgc aaaaccagga actgatttta ctttaccaaa
    21241 aggacgaaca gtccaaaaac cattatcgaa gaaaggatga ggaagaaatg aagaaagggt
    21301 gtctaggttg tcttcttttc tttattcttc ccttttttct attgccattc tttttttcag
    21361 gagaactttc tgagcatgga ggagaagcta caggtctagc tgtcgctttt aatggggaat
    21421 ataccgaagg attaccgatt ttttcagaga ttaaaggacg tggtccaatc acagatgaac
    21481 atgcgcaagc agcagttggt gcagctgtaa agtatcagtt attaccgagt gtgattcttt
    21541 ctcaattagg ctatgaatca gcttatggac agtcacaatc tggaagaaac gatacgaact
    21601 tttttgggat tacctggttt cctggttgtc cctacccaca aggttctgca cgtggaattg
    21661 gtggttctga aggtggtttc tatatgaagt ttccgaactt aaaagcttgt tatgactatt
    21721 atggctttat ggtggcgagc caaagcaact ttaatcaatg tgtgggcaat aaagatcctg
    21781 gaagttgttt attgattcta ggtcgtggag gctatgcggc agcaggtatt accgaaggga
    21841 gtccgtatta tcgaggagca atgagtatca taacgtccta taacttaacg gaatatgata
    21901 cttttgcgat caagcattgg aatagcctac caacaaaaaa aacttcgctt attgcaggaa
    21961 aaagtaaagg caatgcgaag gttttggatc ggatcattgg acgatccgta aataatgggg
    22021 aatgttatgg tggtacggca tggtatgtat ctcaattagg tggcccacag ttaatgggtt
    22081 ctggacacat gtttgcgatg ttaatagggg aagattataa ctggacaacc tatggttggc
    22141 aagtaatcat gaacccaaaa ccaagtgatt tacaagcagg agatgtggtg aattggcaag
    22201 caggtggtgc attgtctcct ggaatctatg gtcacaccgg aattataaca gatgtttcat
    22261 atggcggaca acatttctct agtgtggaac agaatgccga gcaaggacgg attatggctc
    22321 ggtatcaacg cacgtacaat caaacaaaaa ttcgtagtct ggttcgtaag gtgaaataag
    22381 aaaggcagtg actagaaacg tgaaaaaagg gataatttta gtatttagtt ggttatgttt
    22441 gataggtctt actggttgtc attggttttc ttctgaaaaa caaaaaacac aattgagaac
    22501 gacagaagaa acaatcgaag cagtaaaaga aacaagtgga actcttcaaa gtactgaaag
    22561 taacagtcaa attggttcat tagaagacga gacagcgcaa caaatgcaaa tagatgaaat
    22621 gacaaaaaat ttcttaaaag attttgctag aaaatggagt aatttcacag atgtttataa
    22681 acgcaatcaa gctgtgaggg actatatgac agatcgttgt attaaagaca atagtattga
    22741 tgtagacccg catgtacaaa tgaaaacaac tgggaaactt taccaggtga cacaagatat
    22801 agagaatccg gatcaatatt tgttatttgg agaagaaaca atgaataagg ttactcaatt
    22861 tgtattactt caattgaaag taggtcaaga agctcaaaag attgatgcga tcaagattta
    22921 ttatgtacgg agtgcgtatt aaaataaaaa atgaacactc atatttatta tacaccgaag
    22981 aaatagaaaa aaacagactg tttttttaag aaaaactatg tcattcttta cttgaaaaaa
    23041 gttgttatag gacagaaagg ataaaacaaa aacggaaaaa atatgtctta tgaataacta
    23101 taagaactat tctatttttt aggagatgga tacgagatga aaaaactaat tggctatagt
    23161 gtgatgtgta ttggatttgt tggtgtggga ttgtttgtat cgactgaatc tgcttctgcg
    23221 gctgaatggt ataatcctat ctggtataat ccgaacattg aatgtcaaaa aggctatccg
    23281 tatggaccga caaatctgaa aaatggaaaa tgtagagtaa attggaatgg cgttgtagat
    23341 agtgtaatta ctggttcaat ggactctgct ctacatggct ttacgggtca ctagaaggag
    23401 gaagaaaatg tctgaagaga aaaaagaaat cgttgaatca ttagtagctt taaaagattc
    23461 tctttctaag attgaatatg ttgataggca agaaattcaa aaactaattg atgatactat
    23521 tattgaaatc caagatgcgc gttgtgaagg tataaaaatt tctgtagctt tatctaaagt
    23581 aatagaaaaa atgaaccgtt cattagcgtt taatggattg aagctagata gacaaactag
    23641 tctgatatgg gatcatttaa aagacttgta tgacaagtct aaaagaagtg aacggactgc
    23701 tgtatcaatc ttaaaaggcc tttggggaat gaattcataa acaagatatt taaaaagtaa
    23761 cttatttttt atggaggaga aattatgaaa agaataatta aaatatttgg tgttttaggt
    23821 gttttcttat taggtttaag tacttttgtt agtactggaa caagtgtgta tgccacagaa
    23881 aaagtgaata cagttgattt agaaataaag aataaattag tagatgaatt aactttttat
    23941 tttgatacag ttggtttttt agacgaacat ggtgtatacc atataaagga ttttggtgct
    24001 attcgtgaac gaatcagtca agataacgaa caaggaagag cagcaagagt cttttatgaa
    24061 catattattg ttcctagaat ggagaaagtt gattggaaac gatatgctgg atgtgtagcc
    24121 attaattctc ttcctggagg aggagtggct tgggctatag gtacgcaggg agattttatt
    24181 gaagcattaa aagcttttaa ttttgaaaaa gctgcggatt tattattaaa aattgcagaa
    24241 aaacaattag gaaaagctgc agctaaagag ttagctaagg taaatattgt agcaggaatt
    24301 gcagcagctg ctgctagctg tgcattagag taaaagttta tgaaaagttt agaacaagag
    24361 catatggctt ttaaacaagc aatgtttaaa gaaaatatct atttaaacca taactatatt
    24421 cgtgtttcta aggcatgttc accagtattg aatatgcttg gaggtggaaa tggattatat
    24481 catttactgt ttgtcgatgt ttgttggtta atttttttac cagatgaact agttatagtc
    24541 aatgaaaaa
//
LOCUS       DADVXO010000028        24150 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.029, whole genome shotgun sequence.
ACCESSION   DADVXO010000028 DADVXO010000000
VERSION     DADVXO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.029"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="KB950_000970"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="KB950_000970"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBB6857203.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="KB950_000971"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="KB950_000971"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBB6857204.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="KB950_000972"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="KB950_000972"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBB6857205.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="KB950_000973"
     CDS             complement(3373..4680)
                     /locus_tag="KB950_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBB6857206.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="KB950_000974"
     CDS             complement(4683..5264)
                     /locus_tag="KB950_000974"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBB6857207.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="KB950_000975"
     CDS             complement(5836..7128)
                     /locus_tag="KB950_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6857208.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="KB950_000976"
     CDS             complement(7125..7748)
                     /locus_tag="KB950_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857209.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="KB950_000977"
     CDS             complement(7751..8587)
                     /locus_tag="KB950_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6857210.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="KB950_000978"
     CDS             complement(8604..9491)
                     /locus_tag="KB950_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6857211.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="KB950_000979"
     CDS             complement(9507..10790)
                     /locus_tag="KB950_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6857212.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="KB950_000980"
     CDS             complement(11473..12156)
                     /locus_tag="KB950_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBB6857213.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="KB950_000981"
     CDS             complement(12174..12791)
                     /locus_tag="KB950_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6857214.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="KB950_000982"
     CDS             complement(12801..13625)
                     /locus_tag="KB950_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBB6857215.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="KB950_000983"
     CDS             complement(13618..14178)
                     /locus_tag="KB950_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857216.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="KB950_000984"
     CDS             14352..15308
                     /locus_tag="KB950_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6857217.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="KB950_000985"
     CDS             complement(15613..16569)
                     /locus_tag="KB950_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBB6857218.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="KB950_000986"
     CDS             16673..17275
                     /locus_tag="KB950_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6857219.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="KB950_000987"
     CDS             complement(17363..18862)
                     /locus_tag="KB950_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBB6857220.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="KB950_000988"
     CDS             complement(18878..19402)
                     /locus_tag="KB950_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBB6857221.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="KB950_000989"
     CDS             19700..20449
                     /locus_tag="KB950_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6857222.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="KB950_000990"
     CDS             20440..21225
                     /locus_tag="KB950_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBB6857223.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="KB950_000991"
     CDS             21194..21910
                     /locus_tag="KB950_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6857224.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23046)
                     /locus_tag="KB950_000992"
     CDS             complement(22012..23046)
                     /locus_tag="KB950_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBB6857225.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23050..24108)
                     /locus_tag="KB950_000993"
     CDS             complement(23050..24108)
                     /locus_tag="KB950_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBB6857226.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7151 a         5212 c         4213 g         7574 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa
//
LOCUS       DADVXO010000029        23829 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.030, whole genome shotgun sequence.
ACCESSION   DADVXO010000029 DADVXO010000000
VERSION     DADVXO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.030"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(53..787)
                     /locus_tag="KB950_000994"
     CDS             complement(53..787)
                     /locus_tag="KB950_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBB6857227.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="KB950_000995"
     CDS             1114..1524
                     /locus_tag="KB950_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBB6857228.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="KB950_000996"
     CDS             1517..2218
                     /locus_tag="KB950_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBB6857229.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="KB950_000997"
     CDS             2218..3804
                     /locus_tag="KB950_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857230.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="KB950_000998"
     CDS             3823..4044
                     /locus_tag="KB950_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBB6857231.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="KB950_000999"
     CDS             4041..4802
                     /locus_tag="KB950_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBB6857232.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="KB950_001000"
     CDS             5182..5691
                     /locus_tag="KB950_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBB6857233.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="KB950_001001"
     CDS             complement(5766..6305)
                     /locus_tag="KB950_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBB6857234.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="KB950_001002"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="KB950_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBB6857235.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="KB950_001003"
     CDS             7318..8169
                     /locus_tag="KB950_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBB6857236.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="KB950_001004"
     CDS             8197..8832
                     /locus_tag="KB950_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBB6857237.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="KB950_001005"
     CDS             8852..9625
                     /locus_tag="KB950_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6857238.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="KB950_001006"
     CDS             10033..10830
                     /locus_tag="KB950_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBB6857239.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="KB950_001007"
     CDS             10808..11221
                     /locus_tag="KB950_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6857240.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="KB950_001008"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="KB950_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBB6857241.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="KB950_001009"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="KB950_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBB6857242.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="KB950_001010"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="KB950_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBB6857243.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="KB950_001011"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="KB950_001011"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBB6857244.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="KB950_001012"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="KB950_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBB6857245.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="KB950_001013"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="KB950_001013"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBB6857246.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="KB950_001014"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="KB950_001014"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBB6857247.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="KB950_001015"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="KB950_001015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBB6857248.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="KB950_001016"
     CDS             21905..22483
                     /locus_tag="KB950_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6857249.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="KB950_001017"
     CDS             22535..23017
                     /locus_tag="KB950_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBB6857250.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="KB950_001018"
     CDS             23134..23508
                     /locus_tag="KB950_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857251.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4286 c         5115 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DADVXO010000030        23653 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.031, whole genome shotgun sequence.
ACCESSION   DADVXO010000030 DADVXO010000000
VERSION     DADVXO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.031"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(26..601)
                     /locus_tag="KB950_001019"
     CDS             complement(26..601)
                     /locus_tag="KB950_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6857252.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="KB950_001020"
     CDS             complement(606..1580)
                     /locus_tag="KB950_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HBB6857253.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="KB950_001021"
     CDS             1863..2831
                     /locus_tag="KB950_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6857254.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIKNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="KB950_001022"
     CDS             2928..3221
                     /locus_tag="KB950_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB6857255.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="KB950_001023"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="KB950_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBB6857256.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="KB950_001024"
     CDS             3678..4433
                     /locus_tag="KB950_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6857257.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="KB950_001025"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="KB950_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBB6857258.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="KB950_001026"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="KB950_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBB6857259.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="KB950_001027"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="KB950_001027"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBB6857260.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="KB950_001028"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="KB950_001028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBB6857261.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="KB950_001029"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="KB950_001029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBB6857262.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="KB950_001030"
     CDS             9170..9712
                     /locus_tag="KB950_001030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBB6857263.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="KB950_001031"
     CDS             9735..11291
                     /locus_tag="KB950_001031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBB6857264.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="KB950_001032"
     CDS             11305..12207
                     /locus_tag="KB950_001032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBB6857265.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="KB950_001033"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="KB950_001033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBB6857266.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="KB950_001034"
     CDS             13650..14072
                     /locus_tag="KB950_001034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBB6857267.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="KB950_001035"
     CDS             14541..14771
                     /locus_tag="KB950_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBB6857268.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="KB950_001036"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="KB950_001036"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBB6857269.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="KB950_001037"
     CDS             16182..16358
                     /locus_tag="KB950_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBB6857270.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="KB950_001038"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="KB950_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBB6857271.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="KB950_001039"
     CDS             16853..17320
                     /locus_tag="KB950_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857272.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="KB950_001040"
     CDS             17405..18289
                     /locus_tag="KB950_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6857273.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="KB950_001041"
     CDS             18403..18639
                     /locus_tag="KB950_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBB6857274.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="KB950_001042"
     tRNA            18727..18807
                     /locus_tag="KB950_001042"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="KB950_001043"
     tRNA            18814..18885
                     /locus_tag="KB950_001043"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="KB950_001044"
     CDS             19011..19181
                     /locus_tag="KB950_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857275.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="KB950_001045"
     CDS             19401..20108
                     /locus_tag="KB950_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6857276.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="KB950_001046"
     CDS             complement(20167..20811)
                     /locus_tag="KB950_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBB6857277.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="KB950_001047"
     CDS             20938..21225
                     /locus_tag="KB950_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857278.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="KB950_001048"
     CDS             21333..21653
                     /locus_tag="KB950_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6857279.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="KB950_001049"
     CDS             21971..23047
                     /locus_tag="KB950_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBB6857280.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23653
                     /locus_tag="KB950_001050"
                     /pseudo
     CDS             23044..23653
                     /locus_tag="KB950_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         7821 a         4124 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattaaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DADVXO010000031        23455 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.032, whole genome shotgun sequence.
ACCESSION   DADVXO010000031 DADVXO010000000
VERSION     DADVXO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.032"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            44..361
                     /locus_tag="KB950_001051"
     CDS             44..361
                     /locus_tag="KB950_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6857281.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            354..962
                     /locus_tag="KB950_001052"
     CDS             354..962
                     /locus_tag="KB950_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBB6857282.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1017..1808
                     /locus_tag="KB950_001053"
     CDS             1017..1808
                     /locus_tag="KB950_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBB6857283.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1946..2434
                     /locus_tag="KB950_001054"
     CDS             1946..2434
                     /locus_tag="KB950_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6857284.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2485..3357
                     /locus_tag="KB950_001055"
     CDS             2485..3357
                     /locus_tag="KB950_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBB6857285.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3533..3895
                     /locus_tag="KB950_001056"
     CDS             3533..3895
                     /locus_tag="KB950_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6857286.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3904..4656
                     /locus_tag="KB950_001057"
     CDS             3904..4656
                     /locus_tag="KB950_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857287.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4670..6472
                     /locus_tag="KB950_001058"
     CDS             4670..6472
                     /locus_tag="KB950_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857288.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6749..7789
                     /locus_tag="KB950_001059"
     CDS             6749..7789
                     /locus_tag="KB950_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6857289.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7841..9985)
                     /locus_tag="KB950_001060"
     CDS             complement(7841..9985)
                     /locus_tag="KB950_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6857290.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(10342..11715)
                     /locus_tag="KB950_001061"
     CDS             complement(10342..11715)
                     /locus_tag="KB950_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6857291.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(11715..12110)
                     /gene="rbsD"
                     /locus_tag="KB950_001062"
     CDS             complement(11715..12110)
                     /gene="rbsD"
                     /locus_tag="KB950_001062"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBB6857292.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(12138..13055)
                     /gene="rbsK"
                     /locus_tag="KB950_001063"
     CDS             complement(12138..13055)
                     /gene="rbsK"
                     /locus_tag="KB950_001063"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBB6857293.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(13163..14176)
                     /locus_tag="KB950_001064"
     CDS             complement(13163..14176)
                     /locus_tag="KB950_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6857294.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(14257..14856)
                     /locus_tag="KB950_001065"
     CDS             complement(14257..14856)
                     /locus_tag="KB950_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6857295.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15042..15743
                     /locus_tag="KB950_001066"
     CDS             15042..15743
                     /locus_tag="KB950_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857296.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            15753..18464
                     /locus_tag="KB950_001067"
     CDS             15753..18464
                     /locus_tag="KB950_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857297.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            18915..20543
                     /gene="treC"
                     /locus_tag="KB950_001068"
     CDS             18915..20543
                     /gene="treC"
                     /locus_tag="KB950_001068"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBB6857298.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(20603..21784)
                     /locus_tag="KB950_001069"
     CDS             complement(20603..21784)
                     /locus_tag="KB950_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6857299.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            22036..>23455
                     /locus_tag="KB950_001070"
     CDS             22036..>23455
                     /locus_tag="KB950_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBB6857300.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT         7631 a         4195 c         4786 g         6843 t
ORIGIN      
        1 agattgaaat aaaatattac acttgagaat agaaggtgac gtaatgcaca tccagattcc
       61 aactgttctt cttgatggta ctgtgctagc agcattaagc aaggaagata tgtatgggta
      121 tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt gaatctacac tatatccggt
      181 tttgagaaga ttgaaaaaag aaggatatct tgagacctat gatgaacctt ttgaaggcag
      241 aaaccgtcga tattactcgt tgacaccaat gggaagtcgt catttagaag aaattattga
      301 tgaatggatt gtttttcgag attctttaga ccggttattg gaggagagca caaatgaatg
      361 aatatttgaa tgaagttgtg gatcatcttc aagaaattcc tgaagacgat cgttttgatt
      421 tgatccagta ttatgaggaa tattttttag attctggaaa aacattagat cagatcattg
      481 atgaatatgg tacaccaaag cagtttgcct taaaattaaa aatcagttac ttttcagaag
      541 aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt
      601 taatcattat cggtttattt gcttctcctg tcctcattcc actagcactg gggtttatat
      661 tggttattgg gggactattg gttgctttac tagcgctcat ttttagtatc tatgttttat
      721 gtatcagtat cttaggtgtt ggcctattca tgatcatctc aggaatcagt gtgtttggac
      781 aatcgattct gagtggatta ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag
      841 tatttatttc gccactgttg ctgaaaggaa caaaagggct tttttatgtc atgatgaact
      901 ttgttaaatg gatgggacga cgttttatta caaaaagaga tctgcattca gcgggcgttt
      961 gaaggaggac acataaattg agattgaaac attttttgat taccggtatt ggtctgatgc
     1021 ttactggtgg cctcctagca ggtgcaactt atacattagg cgctcaaaag agtctgactt
     1081 gggataatgg tcctcggttg atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa
     1141 acaaaatcgt catccatgca gaaaatcaaa cagtttcggt catacgcgga ttggattttg
     1201 agataaaaac gacttatgat aagacagaaa agccagaaat aaaggagaaa aatggcgtgt
     1261 tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat tagtttagaa gacacaagtg
     1321 cacaaatcac tattgttgtc cctgcagatc atgaattatc tgaattgaca atcgaaggga
     1381 ataatagtgc aattgatatc aacgaagtat caatcaaaaa agtgaatact aagacacatg
     1441 atggctgggt atcgttcaat gaatttacag gaggaacaat aactgcgaaa gatgaattag
     1501 gctcacttcg attagatgag gccaatgtgg ataacttgat cgtcacaggt gaaaacagca
     1561 acttccatct agaagacagt ttgatcagca aagaaggaaa agtcatccta aacggtggca
     1621 atttatcttt gcatgagatg gaaaacagtg gctatactat ccaaacgtct ggtgaatctt
     1681 atattgaaaa agactaccag ccaataacaa atactttgtt agaacaagga cagaaaaagc
     1741 taaagatcac tgctaaagac acatcgatca atattacgac ggatcaagag gacaatgaaa
     1801 cagattgatc ctgtactcaa ctggatattt taagaaaaaa cagcaaagat ccttccaatc
     1861 tttgctgttt ttcatcgctt aacgcacata actatagtaa aataagaaag aaaatatcta
     1921 aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa
     1981 aacagaaatg gaaagattac atacttatat cactcaacta agccaaacct tctatgatcc
     2041 agacaaagaa aaagtgatgg ttaattaccc gaacaattca gaaggtcgcc aattagagca
     2101 agtgtaccat gaagttttca aacatttatt gacggtgaag aaagagctgg attattattc
     2161 tttaccgatt atcgatacag gaatattgaa gtatgatggg aagaaggaac gatttatctt
     2221 taaatctgtt cgggaagatt tgcctctttc agcagggatg gatctagagg tcttggtaga
     2281 agactatttt acagaagaaa agcactgggt tcgaacaaaa ttggattatt taccgcaggc
     2341 agccggaggg acacaggcaa gcggttggta catcactgaa gacaaagagt tagaactaga
     2401 aggtgtaatg gcgcgtattc gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa
     2461 ggataaagga gcgcaggaag tagcatgaga gaaaaaagag cattttatat caatggttat
     2521 gcagggattg tcggattggt cgtattagct ttagttggat tgtttttctt ctatttaggt
     2581 atgtggcaag cgaaagtttg ggcacttttt ttaagtattt ttctttggtt gatcacgtta
     2641 ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag caaaagtaat tttgtttttt
     2701 ggacaatatt taggtacgat tcgagaaaat ggctttttcc ttacgatacc tttggcacaa
     2761 aaaatgactg tttcgttgaa agttcggaat ttcaatagtt ctgttttaaa agtcaatgat
     2821 ttggatggaa accccatcga aatctcagca gtagtagtct ttaaagtcgt tgatacagca
     2881 aaggcattat tcgatgtagc gtattatcaa gattttgtag aaatccaaag tgagacagcc
     2941 attcggcata tcgctagcca atacccgtat gatacattca atgagaatga tttgacgctt
     3001 cgaggaaaca cgacggcagt gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca
     3061 gtagcaggtg tcgaagtaat cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc
     3121 gctagtgcca tgctccaacg tcaacaagca aaagccattt tgtctgctcg acaaaccatt
     3181 gttgaaggag ctgtcacaat cactcaaatg gccttagagc aaatcgaaga tggacaagaa
     3241 atcaatttca ccgatgatcg gaaagtccaa ttgatcaata atttacttgt ctcaatcatt
     3301 acagacaaag gaacgcaacc tgttatcaat acaggcgaca tgaaagaccg taagtgacat
     3361 actttttgta agataggaag aaggagttta ggacggatta ttgtcttgaa ctcttttttt
     3421 agttgtttta aagaaaatga caaaattgaa agaagaatga tagttgattc gaaggcagaa
     3481 ttgttcgctt ttttctataa tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa
     3541 acttctgatc ggtctacttg ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga
     3601 ttacgtagtt atgggtggag aagaatacta tgtacaaatt acgacagatg gtacaaaaac
     3661 agtttcaaaa tcagactctg gacaagaaac agtcaactat agatatatcc tgcctggcta
     3721 tacgaaagag ggtaaggaaa aagaactgga cttcaaggga caattgactc gaccgcttcg
     3781 taaaggagca tatttaaaaa tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga
     3841 agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa
     3901 gaaatgaaca aaatagtcga agtacagaat gtaacaaaag tatacggaaa aaacaatgag
     3961 aaaaagacgc aagcattaag cggcatcagc ttcgatgtag aaaaaggcga atttattggg
     4021 atcatgggcg cttctggttc tggtaagtcg acacttttga atattttatc cacattagat
     4081 aaaccaacag atgggcatat ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat
     4141 caactagcag attttcgtgc gaatgagatt ggctttattt tccaagattt caatttatta
     4201 gaaagcttga cagcacaaga aaatatcgct gttcctcttt ctttacaagg cgtcagacca
     4261 aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt tatccatctc tcatattttg
     4321 gaaagctatc cttctgagat ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca
     4381 ttgatcacac aaccaacgat tctattaggg gatgagccta caggtgcgct ggattccaaa
     4441 agtgcccggg atcttttaga tacgatggac gaattgaata caaaagatca tgtatcgatt
     4501 cttcttgtga cgcatgatcc gttttctgca agttattgcc aacgtatttt attcatcaag
     4561 gatggcggta tccatcaaga agtgaaacgt ggagatcaaa gccgtgattc cttctatcga
     4621 gaaatactat ctattcttgg aaacttggaa cagtaggggg agattgacta tgctttggaa
     4681 attatcactt actgggatca agagccgttt aagagactac ttggtgttat tctcaggatt
     4741 agttatggca tctgctattt tttacatgtt tgaatcaatg gcaagcaacg aagctttttt
     4801 gaaaagtaat acgatcgttg cggctacagt tatcattttc cgattcggtt ctgttttact
     4861 agggatcatc acattcgttt atattttata tgcaaattcc tttttgatga cgatgcggca
     4921 aaaagactat gcaatgttta tgatgctggg agcaaaaggc agaaaaatcg cacagatgat
     4981 tttttctgaa acattcttag tagggatcgc agctacttta gtgggttcag ctgtcggtgt
     5041 aggattgaca agtatcgtcc accgattatt agtggatcag ttagatctca atatttcgca
     5101 ttttacacct tttagtattc acgggttgtt gatcactgtt ttgttttttg ctgttttgtt
     5161 tttattagct gctgttgtca atgctttttc gattgtcaaa aaaccgattt tagcattgat
     5221 tcgtgcggat cagacaccaa caagaatcaa gcagcataaa ttcttgtttt tccttgaagt
     5281 agtactggga attgcttttc taggctgcgg ttactatatg atgaaacacg tggggaccta
     5341 tcaagtcttt ggtatagggg tagccttagt tacgatcgta ttaggaactt atttcatttt
     5401 ccattctgtg atcattttct ttttatcatt actaaagaaa tcagaacaga tttcattgaa
     5461 aaaattgaac aatttcacat tgtcacagtt aaactttcgt attcgtgaat atacacaaat
     5521 gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc gctttgacgg taggattagg
     5581 ttttaaaaat gaaattgcag acttgacaga aaacaattct ggatatgatc tagtgatgaa
     5641 taatcctcgg gcagaagatc agcaaaaaat caaagaactt tcaccaacac tttctgcttc
     5701 ttatactcaa aaagaagatg cgggaacgat ttactataat gcaagtgaat ttgcggctca
     5761 accattgatc atgataaaac atgaagatgg aacaccgccg aagatcacga aagaaaaagt
     5821 tcctgctaaa aaaatgaatg aactgactgt acaagaggaa cttcgaagct atgaattgcc
     5881 ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca gaatttgatc aactgaactt
     5941 gccacaatcc actttacagc tagttcaggt aaaagacttc caagcaaacc tgaaaccaat
     6001 cgaaacactt gttgctcaaa ataaaatgaa caatccatct ttgcaaaaag tagagtacag
     6061 caaccagaaa tatgaaatgt atgaagtgta taatggtcta ttctctggtt ttgaatttat
     6121 gggattcttc ctaggtttag ctttcttgac catgcttgct agctgtttga tgttcaagat
     6181 cttgtcagga agcaagagtg acattgctcg ttatgaaatg cttgaaaaga taggaacacg
     6241 acgcgggttg cttaaacaat cgatccgtag agaagtaggc gtgttattct tagcaccagg
     6301 aatcttaggt gtagtccatg tcttattcgg attacaaatg tttggtctat tcatgcaaaa
     6361 tccatacaaa gatatttggg ttccgtttac gatattcttc gtattgtatt tcatctacta
     6421 tgtactgacg acatggttat atactggaat cgtattgaaa aagagattgt gaaaaagctg
     6481 tcctgcatca agtaataaga acagccaagc aaaaatagct cctcatattt ttcgtttggt
     6541 tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaaaa agaggttgtg
     6601 acaatatttt tgtcacaacc tttttttcac ggtcttttat tagaatatag taaatttttt
     6661 ctcgttactc acggaataag ctgttaagaa aacggtttcc atgtatactg gaatcagtgc
     6721 aaatgaaaga aaagagggtg caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa
     6781 ctatcacttg attacgatca cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg
     6841 ggcacgggtt gttcattttc aaacaataat cgataatgaa aaacgagagt tgatcttagg
     6901 ttttgatgaa gcaaaagaat atctagaaaa agacccttat atcggtgctt caatcggacg
     6961 tatggctgga cggattacga atggcacatt caaattagat gatgaaacgt tcgaagtaaa
     7021 aacagacccg gacactggtc attgtttaca tggtggtgca cctagctttg aagccaagca
     7081 gtggcattat acagtcatca atggtgaaaa cgaagcatcg gtcattttct atcagactag
     7141 cccagatatg gaaaacggat ttcctggaac tttagatgta gaagtacggt atacgctgac
     7201 gaacgatgat acatggcgag tgacggtcca agggatcagt gaccgcaaaa cagtattcaa
     7261 tcctacaaat catgtctatt tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca
     7321 tatactttgg ttgaatagcc gattatttgc tacgctcaat aaagatacaa cgcctacagg
     7381 agaagtagtt tcagtaagtg gcacagcatt tgacttccaa cgtcctaaaa aattaggtga
     7441 ggtctttgcc agtgattttc gccaatcaat gatagtgggc ggcatcgatc atccattttt
     7501 gttgaaaaaa caatctggtt tactaaatgc tgcggaattg acaagcccag atgaaaaagt
     7561 atcgatccaa gtaaaaacag atgcttcagg aatcgtgatt tttacagcaa attttggaga
     7621 agatggaccg gaaatgcgcg ggaataaatt agtaaatcat ggcggtatca catttgagac
     7681 tcaagaactg ccaggagcgg agcgacttga aagttttgga gatgtcactc tggaacctaa
     7741 ccaagtacgc gagtttgtaa cggaatacaa aataaatata aagaaataga ataaattaaa
     7801 aagggagtgt gacagtgtca catccccttt ttaatttatt ctatttcttt aaaggcagat
     7861 caagccagaa tgatttgtct aaccttaaca aggttagtac aggaacagtt gcagctttaa
     7921 ctgtacgagt agttgtttct ggtatctgtc ttgccgtaaa attgttccac aaatcccaga
     7981 ttgaagcgac acggaagatg gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta
     8041 atcctgtgat tggcacgcca tctgttgtcc atttgattgg cacatcgaat cgcttagaca
     8101 tattgaggat ttcagcgttt gcttcttcag ttgttgctgc ttcttttttc ggttcatcat
     8161 attttgtcag gtcgtacgct tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg
     8221 tagcataacg tccttccaaa tatttggttg cttctttgta gtcttttgtt tccgacttcc
     8281 aagttggttt gtagaaatca ggattgccag tcagtccatt tttgatcaag tcggcatagt
     8341 cttccaaact ttccttgtaa gaaggatatt tacggaattg tgcgcggatt tggtagcttt
     8401 gtccggaact atcttgttca agtgtattga aggtcacgct gcttccttta tatgatcctt
     8461 tgataccaaa aaggttatga tttggttcgc ttgataataa gctgtttcca gatccacttt
     8521 caaggatagc ttgagccatc atgactgatg cgtagattcc ttctttttgc ccgatttcct
     8581 gtgcatcatc tgcaatcttt ttcaagaact gatcagttgt ttggttttga ctgaaatgga
     8641 acgagccatc taatccatct aaaatagatg agccgcctgt cagcgaacct gaagcagcag
     8701 gtaaacctgc taagtttact cggacagcaa aatcaggcgt gaaatctgaa atagaagtta
     8761 ctcgaacttt atctcctggt tgaggcgcat ggataaattg tccgttccca atatagatac
     8821 ctacatggtg agtaacaccg gaagaaggtc cccagaagac gaggtcgcca ggttgggcct
     8881 gacttactgg aatctgcgta cctgcatatt gctgttcgcc tgtccagcca ccgatataat
     8941 gaccggttgc tttcatatat acgtaataag tcaaaccaga acagtcaaaa gtatttggac
     9001 cttttgctcc ccatacatat ggttttccaa gatgcttgaa tgcttctcga acgattgctt
     9061 gttggacatt cgttgtattt gtaggcaata cagcatcaat tggtgatttt acaacaaaat
     9121 cagaagaagc agaagatgga ttctctatac cgctttgatt tgctgtattt ggacgagatg
     9181 aagcattgtt cgttccgcca ttcgatgaac cattgtttgg atggtttggt tctctctgag
     9241 tatttccatt gttattcgat ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg
     9301 aattattgtt attattgttt gaattatttg aaccattatt attgttgtta ttacccacat
     9361 ctggtttgtt tggcgtttcc ggatctggtg tactagggac ttcttcacta ggatcagttg
     9421 gtttctctgt tgaagtatcc ggtccagctt taaattctgc agtaatagat aaattgttcg
     9481 tacctactgt aaaggtcttt gcatcataat ttacttcttt ggcaccatta acagcgactg
     9541 aagcaatttg ttcaccttca gctggcgtaa tagtgtaagt taccttcgtt tccgctaata
     9601 aaccttctac cgttccactt tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg
     9661 tcgaagcagt cagttggatc ttaccaatca aagaaggagc aattgtaagg gaatatgtct
     9721 cggctggctt ttcattttct gatggatcag ccggatcaat ttcagttggc tcggtcgatc
     9781 ctgttgttga gttagttgtt ccagttgggt cttctgtaaa agaatccgtt gtttcactag
     9841 tagctgagct ggatgtagtc gtatctgttg tgactgtttg atcagctatt tgattatcta
     9901 cggcaaatac ggtattggtc gacaaaacag acggagcaat cagccaagta gcagctagca
     9961 aacgtatcgt ttttttattt ttcataataa actagtcctt ttcctttcat tgattttctg
    10021 tcttattcag cattcattag acattttttg gaacagataa tcgtgtcgaa aagctttttc
    10081 ttttcataaa tctgtctgtg tattctaaat aaaacaattt gtaaataagc aacgagcatt
    10141 cattcgtttt tctttgacgt tcattttatt gcatagacca cctttgtttg gggataaaca
    10201 agtggggata aaaatggtta tgcatatgta aattatacca aagtttggaa agtatcacaa
    10261 aaatttataa aaataatgat cctaaaaaga aaaagactga aatatgtgat aaacatctca
    10321 tacttcagtc tttttcatta actattcata agacattagg tcttcatcaa acccgctaac
    10381 gagagtagtg atcattgcta cgactagact aacagccagt cctgctaagt accagaaaaa
    10441 agtaccggaa atgaatgcag gcaatgtggt cagactacca aatacaaaag cgatcgcttt
    10501 cacttgggca aatcccatta cagctccacc agcagctgca ccaatgcaag cagcaacaaa
    10561 tggttttttc aatgggatcg caattccgta aacagttggt tcggtcactc ccgcaagtaa
    10621 aatcgaagca aaagcagaac cggagatcga acgggttttt gaacgtttac ttcgcagaaa
    10681 tacacctaaa gctgcaccag ccattccaaa attggctgct cctgtcaacg gaacgattcg
    10741 gtcgaaccca taagcagaga tattactgat catgacaggg tttacagccc attggatacc
    10801 aaatattacc aaaatattcc aaccaccagc gatgattgcg ccactgatcg gtccgttaat
    10861 atccatcatc caagtaattg cggcagctaa accttcgcca aggtaaacac ccgcaggccc
    10921 gaataccatt aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta caaatactaa
    10981 ttgcatattt tcgcgaatat attttttgag gaatttttca acaaaagaat aaaagaaaat
    11041 acctaaaatc gccggcatga cagtagacgt atactgcatc agtactacag gaatccccag
    11101 aaaatcagta gctgctttgc cactgtctga aagtaaagag gtcagattcg gttcaatcag
    11161 tgctgcagca atgacagccg caatatatcc attcacttta aacgttttgg cagcagaaaa
    11221 agcaagaaag attggcagaa aataaaagac ggcattcgac gcagcagaca aaatatgata
    11281 agttccgctt gtttcactaa gccagccaat cgtagtcgct aaaacaacca gaccttttaa
    11341 tataccagaa ccggcaaaca gcggaatgat cggcgtaaag atttccgata tcaaaccaaa
    11401 tagtttttcg atcggattta gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa
    11461 ttccttgtaa gcaggagcca atccctgatc catgattatt tctgcatatc tatcgagtaa
    11521 aatgaaatac ttaatggaag ggatttgctg ttttaatttt tctgcattgg ctactggctg
    11581 ttttttcaat ttgatacgaa gccgagtcgc acatggaagg acagaatcaa tattgtcttt
    11641 tcctcccaac tgttcaatca gcatatgaat ctgctcggac agtaccatct gttcaggatt
    11701 cattgtcgtt tccattagaa gaccactcca ctcactaata taatattggc gtaaggtgtc
    11761 atttcgcctg tacgaataaa tgcatgacag ttattcaact cttttttcat ttcttcatgt
    11821 gggataaagc taatcggtgt atctggtaac aataatttga ttttttccaa tatttccggg
    11881 ttcattgtct ttatttcttc cgccaattca atcgattgta cctcaagttc ttctaaaacg
    11941 ttttctaaaa cagataaaaa acttggaaga ccgtttgaga cagccaaatc tattttttca
    12001 gtaccaaagg ggacaggcat gcctgcatcc ccgatactta atttatcaaa gtgccccatt
    12061 tgtgcgatta ctcgtgaaat atctgaattg attacttttc cttttttcat gtttgtttct
    12121 tcctctcatt gttaatttta gactgctgat tgttctaaag cttcgcggta agggatagaa
    12181 ggttgcgcac cataagactg cacagcaaca gaagaggcta gatttccata aaggatcgct
    12241 tctttcaagt tactaaggtc tggctgaagt gtgctgctta atgcgccaat aaatgtatca
    12301 cctgcggctg ttgtgtcaac ggcatccact ttaaaagcag ggacgatacc gtgttctttt
    12361 tctgtatgat agaaagcgcc ggcactgcct agtgtaatga ttactgttcc aatacctaat
    12421 tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct gattcctgta
    12481 atgatttctg tttctgtttc atttggtata atgatatctg tcttttcaag tagttcagta
    12541 ggaatcgttt cacgagctgg tgcaggattc aggatcgttg tttttcctgc gtcttttgca
    12601 atagaaaaag ctaggatcgt cgcatctaag ctactttcaa actgagcaat aataaagtca
    12661 ctgtttgtaa tcagttgctt gttttttagg acttcttgtt catggaaagc attatttgcc
    12721 cctgcatgga tcaagatact attttctcct gctgcgtcta ccataatcat tgcttgtccg
    12781 gttgtggcgc cttggatttc agctatccca gatgtatcga tgttttcttt tgtcagtaga
    12841 tctaataact gttgtccttc gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg
    12901 cctgaacgtt tggctgccac tgcttggttg gcacctttac ctccgccagc gtgaaaaatc
    12961 tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc
    13021 atattaatac ttccaattac tgtaaccgtg ttcattttac aaacctccaa ctatctcatt
    13081 tattttgatg ggaaaattat tttttaaaac gttttagtaa aacgttttaa tagttcgata
    13141 aaaatatagc acaccttgaa agctatttca agggtgcagt tgaaaatctt ttttctaatc
    13201 gtacaggtaa tcttttttct tcccattctt tttctgggaa ttggatccga tctaacaata
    13261 acttggcaga tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc agtttcggct
    13321 tgatatactc gcacatatct atattatcat agccaataat gctgtagtct tcagggatgc
    13381 tttttccagc ttcctctaat ccgcgatata atccaaaagc taattcatca tttaaagcaa
    13441 acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg
    13501 agaactgtgt aggtaagagg atcagttggt cgtgagggat ttggtaaaca tcaagagcag
    13561 atttaaaccc ctcaatccga gcgtggacat tttccggtgg attttcagga tagacaaggg
    13621 cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat
    13681 cgtctgttcg tacggcatca ctaaatcctt cagatttttt ctgatccaaa acaatgaagg
    13741 gccggccttg ttttttcaaa ttttcgttga ttgcatctgt agagacagca gaagtcgcga
    13801 taatgaatcc atccacacct cgacgtgtga gctcggctag atattcgatt tctttttgat
    13861 gatcagaatc agcattacat aatatcgtga cgaaattctg cttatacaag atatcctcaa
    13921 ttcctcgcat cagtgtactg aagaatggat tcgtgatatc aggaacgagt acaccaattg
    13981 ttttcgtttc cttggtaatc atttgtctag caaaatagtc aggctgatag cctaattctt
    14041 ctttggcagc taaaactttc tgaacagttt tcggactgaa tttctccgta tttccattta
    14101 atatcaagga gacagttgtg attgaaacgc cggaaagttt agcgacttct ttgattgtga
    14161 tctttttttt caccatgttc atactcctat ctaaatcctg tggttatttt cccatgaaat
    14221 caaaaagaag taaatgaaaa tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc
    14281 gattatttgt ttcgttgagc gtactcgtgc agttttcgga aatatctttt ttatgaacaa
    14341 ttgatgggat tctttgtcac gatcactgca agcatcttca gcaagcacga tatggtaccc
    14401 gtgttgaaag gcatctaatg cagtagcata tacaccgttt gttgtcgcta caccgcttaa
    14461 aatgatagtt tcgattccac gccggcgaag ttgaagatca agagaagtac caaaaaaagc
    14521 acttggattg tattttgtga tagtcaacat attatctgta tctttcaaag aatccttcaa
    14581 aagtaaatca gtgaattctg gaggtacaga agcagtcatt ccacccatat cagtttgttg
    14641 actcaaataa gggaaagtgg agatgtctac gttcactaaa actttcaagg cagcggtacg
    14701 tttcaatgtt cttactaaca aatcattgtt tgtcagaata gatgctgcag tgtgtggaga
    14761 gagtgtaccg ttatttacga tctctttttg catgtcgatc gcgacaaaag cagtttttga
    14821 taaatcaaaa atcatatttt cctttaaatc attcatgatg ttccttcttt caaataacaa
    14881 tgttttttat gtaagtaaat cataaatcgt ttatttgtca actaaattaa aggattcctt
    14941 tctttagtct agagaaattc agtggtaaaa taaataagtg tgagaaaacg cattcgatgc
    15001 atagatatag cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa
    15061 aaaatgaata caaacgatac aaaatgggag aaacgaccat tacagcaaat gacgatattt
    15121 ctttttcaat tgaaaaaggc gaattagtga ttatacttgg tccaagtgga gcaggaaagt
    15181 caacgattct taatatttta ggtgggatgg atacacctga tgaaggtcag atcattattg
    15241 atgacacaga tattgcgcaa ttttcagata aacagcttac tgcttaccga cgaacagatg
    15301 tagggttcgt tttccaattc tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg
    15361 aattggcaac ggaagtttct cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg
    15421 gcctagctca tcgattgaat aactttccgt cccaattatc aggaggcgaa cagcagaggg
    15481 tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat gaaccgacag
    15541 gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac gctagcagac
    15601 aacacggcaa taccgttttg atcattacac ataattctgc tttagcgccc atcgctgata
    15661 gagtcattca cattaatgat gctaaagtaa ggtctgttga attaaatgat catccttcat
    15721 ccattgatga gattgtatgg tagggggatt ttatgaaaac aagaacatat cttaaagcag
    15781 gttttcgcga aattcggcaa tctaaaggac gattcattgc aatcatcctt attgtcatgc
    15841 taggaacact tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg
    15901 atcactatgt tggtacagca ggactttctg atttgcaaat cgtttctact ggtggcttga
    15961 cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac
    16021 agatctatta ttccaatact ggcaaaaacg aagtgattca aatattttct tataacaaaa
    16081 ataacaagca gaacaagcta caattaactg atggaaaact cccggaaaaa ccaaaccagc
    16141 ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt tatcagattg
    16201 attcagatga attgaaagaa aaagagtata ccatcacggg ctttgtccgt tctccattgt
    16261 tcatcaacaa tttggagaga ggatacgcga acgtcggaaa tggtagcgtc gattattttg
    16321 tctatttacc tgaatccgtt tttaaaacag atatttattc tgtcttatat cttgatttta
    16381 caaacgtaaa gaatttgaat acgtacagca aagcttacaa agataaaatg gagcaaaatc
    16441 aagaaaaggc agaaaagtat ttacaagatc gacccgaaga acgcttagaa gagctgaaaa
    16501 aatcggccaa cgaagaatta gaaccggcac agcaaaaaat agcggatggc aaagcacaaa
    16561 caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa
    16621 ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag
    16681 aagctcaact tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg
    16741 cagaggaaga attgaatcga aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc
    16801 tatacaatga acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta
    16861 tcgctgcgat tgccaatgtt tttcctgttt tctttttctt tattgccgca ttgatcacct
    16921 ttacgacaat gacacgtatg gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt
    16981 taggttatac gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag
    17041 ggataggtat tgttttagga acgatattag gaacgaatct tttgccaagg atcatttttg
    17101 agctgtctaa cgagcggtat gatattggga gtgcgcttat tttttatgac tggccgccga
    17161 ttttacaagc agctgccgca ttcttcattg ccgcatttgg cgctgcaatg atcgttttat
    17221 tcaaagattt aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg aagccaggga
    17281 aacgcatttt tcttgaatac atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa
    17341 tcagttaccg aaatctcttc cgatataagt caagaatgct tatggcgatt atcggaattg
    17401 ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa agattctcta agttctgttt
    17461 cagccaaaca atttggaccg atcattgact atcaagcaat cgttacattg gattcatcca
    17521 cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga
    17581 tcctagctat tcatactcaa accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc
    17641 tgacagtgat cgtaccaagt aaaacagctg atttggatcg ttttatccat ttaactgatc
    17701 ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag
    17761 aactatttga cttatctaca ggagatcata tttcgcttta tgatgaagat caaaaagaat
    17821 ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg tagctttatt tatatgtccc
    17881 ctgaatatta tgcttcagtt actgatcaat cgatgatgga taatgcattt ttgctgcgta
    17941 cagataagat gacaacaaaa gaagaagaaa tgctttccga gaaactgctt gccacagacg
    18001 ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga
    18061 acttagactc ggttgtctta atctttgtcg tattatcagg actgctggct tttatcgtct
    18121 tgtataatct aactaatatc aatatttccg agcgtgtacg cgaattatct acaataaaag
    18181 tccttggatt ttttgataaa gaagtcacga tgtacattgt tcgagaaaat atcatcttta
    18241 ctcttttagg aatcatcgga ggatttggtg taggatatat attgactgac ttcatcttgc
    18301 aacaagcgtc tatggagaat gtcatctttc cattagtgat cacatggtgg gcttatgtct
    18361 tatcagcagg attaactatc gtatttacgg ttattgtgat gattgttacc catttcaaat
    18421 taaagcatat cgatatgatt gatgcgttga aatcgaatga ataaaaacag aggttgtgaa
    18481 atcagcggtt tgacctgagt attagagaaa caaacggcaa aatagttttt catattttgc
    18541 cgttttttga gctaataccg aaggacagct gattgaacac catgaatcag aggttgtaaa
    18601 aaagccgtcc tgcatcgagc aaaaagaata ctcaaataaa attagctcct catgttttac
    18661 atttgagaag actgattgcc gtagataaaa gcttttgaac accatttata gaagaaacaa
    18721 gaatattttg ctattttaaa caagaggata gacaaataca tgtctgatct cttgtttttt
    18781 attgggcaac agacctctta gaatgtataa agataaattt tacaaacgct ttcggttcag
    18841 ttataataat aaatgataac ttgtatatat aagttgtggt aggtaataaa tcagttgaat
    18901 ggaggtaaat atccatgaca tttaaagaca aagtcattta ccaaatctat cccaaatctt
    18961 acaaagacag taatggtgat ggaattggag atttaaaagg aatcaaagaa aaactcccgt
    19021 atctgaatga tctaggcatc gacatgattt ggctaaaccc tatttatcca agccctcaaa
    19081 aagacaacgg ttatgatatc tctgattaca cagccatcga tcctatcttt ggtacgatgg
    19141 aggaattcga acagctaata gccgaagcaa agaaataccg gatagaaatc atgctcgata
    19201 tggtgctgaa tcacgtttcg acagaacatg aatggttcaa aaaagcacta gcaggggacc
    19261 agcattatca ggacttcttc attttaagag aagagccaac ggattgggta tcaaaatttg
    19321 gtggaagtgc ttggtcgcca tttggagata cggggaaata ttatttgcac ttatatgatc
    19381 ggacacaagc tgacttgaac tggcggaacc aagcagtaag agaggaacta ttcaaagttg
    19441 ttcgtttttg gatggaaaaa ggggtaaaag gattccgttt cgatgttatc aatgttatcg
    19501 gtaaagacga acaattgaaa aataataaag aaaatgatgg caaacctgaa tatacggaca
    19561 aaccaatcac ccatacgtat ctaaaagaat tgaaccaagc aacgtttggg acggattcca
    19621 ctatcatcac agtcggtgaa atgagctcta cgacgatcga aaattgtatc ttgtatacag
    19681 aacctagtag agaagaatta tcaatggtct ttaatttcca tcatttgaaa gtcgattatg
    19741 aaaatgggaa taaatggtct actcctccat ttgattttgc agaattaaag cgactatttc
    19801 atacatgggg agaaaagatg agtgagggta acggctggaa tgctctattt tttaataatc
    19861 atgatcagcc acgtgcgctc aatcgatttg tagctatcga tcagtatcga aaacaaggag
    19921 cagagatgct agcaaccatg atccatttga accgtgggac gccctatatt tatatgggag
    19981 aagaaatcgg catgatcgac ccagactatt catcgatttc cgactatatt gacgtagaaa
    20041 gtttgaatgc ttataagtta ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc
    20101 ggatcaaagc aaaatctcgc gataactctc gaacacccat gcaatggtca gacacagaac
    20161 aagcaggatt tactacaggg aaaccttggt taaaagttgg aggcaagcta gaagagatta
    20221 atgtagaaaa agaactatct tctgaagatt ctattttgtc ctattacaag aaattgattc
    20281 ggctgagaaa aacatatcca atcgttgcac aaggagatta tcatgcttat gaagcaaatc
    20341 atccggaagt ttatggcttt cttcgccaat ttgaaggaca gcagctgctt gtattaacaa
    20401 atttctatga taaagataca aggatcgcca tcccagaagg attcacagaa gccaatattc
    20461 ttatcgataa ttattccgat actgttcttc atcaaagtct ggagctaaaa ccataccagg
    20521 caattgcttt gctgaaaaat taaaacaaaa acacaaaacg tcgttcctac gagctattct
    20581 cgtaagatcg acgttttgtg ttttatttaa aataatcttc ttcagaaaat gaagagtttt
    20641 catactctcc tttatcgcca taaaatttat tgtagcgttg tttgaattta cggaaaataa
    20701 aagtaacgag taatttgact aacgtataga taggaatacc aaaaataacg cccacaattc
    20761 ccattagatc acccatgaca agtaaaacga ttaagatcgt aatcggatgg atctgtaaac
    20821 gatcacccat tactcgagga atcaccaagt caccgtggag aagttgtacg gcaaaccaga
    20881 caatgacaaa tttgacagcc ataaaagtag agtcttggaa tgcaatgatc aaacctggaa
    20941 agaaggcgat aaacggccca atatatggaa tgatacataa caacccagca gaaatagcta
    21001 aaacactagc aaattctaat ccgatcagta agaaacaaac ccagtagact gctccaagga
    21061 taaatgaagc aatgatttgc ccctttagaa atgaacccaa ttgttgatta gcaatctttg
    21121 tcaaattttt aaaatctgta cgaaatgctg gtgggacaat cgctaaaact gactgataaa
    21181 atttttgagg atctctcaaa aggaaaaaag cgacaattgg accagtaaat aaagtaataa
    21241 aaatagctgt aacagttgaa aaaatgttgc tgatccgttg tgcgccactt tcccaataat
    21301 ttccaataaa tccagctata tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa
    21361 atgaatcggc aaaaggtgtt ttctccaaaa attgtcggag actctcttga aaatcttgaa
    21421 acaaatctgg caaactactg atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc
    21481 atatgcttcc aagccccaat aaaaataaaa taatcacata aacgataatg actgacaagt
    21541 tcctcgaaag gtgcttgctc aaacgtttga tgactggttc caaaagataa tacaagatca
    21601 gtgcaaacaa taatgggggc aagatagtag cgataatgat tccgacaggc ttgaatataa
    21661 acgagatctg atggaaaaaa taaatgatca gcgcaagtaa gataactatt cctaaaacat
    21721 agtaagaggt ttttcctcct aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt
    21781 tcactctttt tcctccttta tctataactt cattatagac aaaagcgggt ataaaaggaa
    21841 aatgatagag gttgattgca aaaatatcgt caatcattca tataaacttt ctcatcaatt
    21901 aaaataagaa aacgcttgac agttctttta accatatgat aaattaaatc ctgttgctaa
    21961 aacgtaacta ttacggtttg cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga
    22021 agaggagtgt cataaatgaa acgattgata ggtatattgg ccatgctggt tttagcaggt
    22081 atgttaactg cttgcggggc aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg
    22141 atgacgacct tttatcccat gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa
    22201 gtagagttat tgattccagc agggactgat tcccacgatt atgaaccttc tgctaaagac
    22261 atggcaaaga tccaagatac agatatcttt gtttataatg atgaaaacat ggaaacatgg
    22321 gtccccgcaa tccaaaaaac attacaagaa ggcaatgtcc atacgataaa agcgacagaa
    22381 ggtatgcttt tacttccggg aagtgaagaa ggacatgatc atgaccatga acacggggaa
    22441 gaaggccata cacatgagct agacccacat gtatggctag ctccaagttt agcaatcaag
    22501 caggtagcaa acatacgtga tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg
    22561 acaaaaaatg cagcagccta taccgagaaa cttcaagcac tccatcagct ttatcaagaa
    22621 acctttaagc aagcaaaaca aagatctttt gtcacgcaac atgctgcatt caactatcta
    22681 gcgttagaat atggattgaa tcaagtatcg attgctggtc tttcttctag tgaagaaccg
    22741 tctgcggcga gaattgctga actgaaacac tttgttaaag aacatggaat caactatatt
    22801 tattttgaag aaaacgccaa agatagtatt gccagaacgt tagcaaacga agcaggtgta
    22861 tctttagaag tattgaatcc tttggaagga ttgacgaatg aacaaataga aaatggagaa
    22921 aactatcttt ctatcatgga agcaaacctc gaagcgttga aaaaaacgac agagacagaa
    22981 aacccgctag aagatacttt gacccccaaa aaagaaaaga gcgtctacaa cggttatttt
    23041 gaagacgagg atgtacaaga tcgctctctt tcagattggg caggaacttg gcaaacagta
    23101 gatacgtatg tagaagatgg gacttttgat cctgtttttg aatataaaga aaaattgaat
    23161 caggataaga cagcacaaga atataaagaa tattatacga agggctatca aacagatatt
    23221 gcttcaatca agatcagtga gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa
    23281 cgttcaaatt atcgttatgt agggaaagaa atcttgacct acgaggcagg aaaccgtggc
    23341 gtaaggtatc tattcgaagc agaagataag gaaagtggcg cttttagata tattcaattc
    23401 agtgatcaca cgattgcacc ggctgactcc gaacattttc atttatatgc aggaa
//
LOCUS       DADVXO010000032        23434 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.033, whole genome shotgun sequence.
ACCESSION   DADVXO010000032 DADVXO010000000
VERSION     DADVXO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.033"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="KB950_001071"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="KB950_001071"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBB6857301.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="KB950_001072"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="KB950_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBB6857302.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="KB950_001073"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="KB950_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBB6857303.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="KB950_001074"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="KB950_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBB6857304.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="KB950_001075"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="KB950_001075"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBB6857305.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="KB950_001076"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="KB950_001076"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBB6857306.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="KB950_001077"
     CDS             complement(8297..8767)
                     /locus_tag="KB950_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBB6857307.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="KB950_001078"
     CDS             complement(8813..9238)
                     /locus_tag="KB950_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBB6857308.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="KB950_001079"
     CDS             complement(9305..9574)
                     /locus_tag="KB950_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBB6857309.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="KB950_001080"
     CDS             complement(9585..11180)
                     /locus_tag="KB950_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6857310.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="KB950_001081"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="KB950_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBB6857311.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /locus_tag="KB950_001082"
     CDS             complement(14733..15293)
                     /locus_tag="KB950_001082"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBB6857312.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="KB950_001083"
     CDS             15646..16620
                     /locus_tag="KB950_001083"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBB6857313.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="KB950_001084"
     CDS             16790..18190
                     /locus_tag="KB950_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBB6857314.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="KB950_001085"
     CDS             complement(18268..18990)
                     /locus_tag="KB950_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6857315.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="KB950_001086"
     CDS             complement(19069..19575)
                     /locus_tag="KB950_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBB6857316.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="KB950_001087"
     CDS             19698..21497
                     /locus_tag="KB950_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBB6857317.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21991..23379
                     /gene="ltrA"
                     /locus_tag="KB950_001088"
     CDS             21991..23379
                     /gene="ltrA"
                     /locus_tag="KB950_001088"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBB6857318.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6646 a         5056 c         4323 g         7409 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ttgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgatgtgcg ccctgcatgg gtgaaaactt ggtggtgaaa gtccactaca
    21601 ggcttggcag taggaactgt tagcgaaaga caaggcggca atcgtgaggt tgtggctgaa
    21661 ggaagtcgga cgcaaactcc tgaactgacg aacagaaact agatacaagg ctgaattagg
    21721 tcggataagt cttcatcaca agacaaagtc caatactgcc cgaaacctat acagtaaatc
    21781 tagcagttac atgggaggaa agttttcact cttaccgggg gagatctcat gagggtgttg
    21841 tgggcgttga atgaaaacaa accagcacaa cgatcatggc gacatgatgg tgaatcatga
    21901 gaagtcagcc gaggtcatag tagtaacgac actgaccgtt acgaaggact gaacaataag
    21961 aatcttgaat aatttaggag gtgtgagaag atgcgacgac cgcagaaaac aagaaaactt
    22021 ggctatcatc aaagagctag tgtggaacac gaagggtatg atgaagtgcg cagcacttct
    22081 tgcggtgaac agaggaaaca agatggtgta gaaaatctgt ttgggagaat tctagatcgg
    22141 aataatctca atcaagcgta tcttcaagta gtcagaaata aaggggcggc tggtgcagat
    22201 gggatgaccg tcgatcaact gcttccttac ttgaaagagc accgcgagga attactatcc
    22261 aatttacgcc aagggaacta taaacctcag cctgttttgc gagtagaaat accgaaacca
    22321 aacggcggtg taagaaaact aggaattccc acggttattg atcgtatgct tcaacaggca
    22381 atcaatcaag tgttacaacc gctatttgaa ccagaattct cagataatag ttatgggttt
    22441 cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa aatcttatta tgaacaaggt
    22501 taccgatatg tggtggacat tgatatgaaa gcctactttg acaccgtaaa tcacgataag
    22561 ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag tcctaaaact gatccgtcaa
    22621 tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa caactgaaga aggaacgccc
    22681 caaggaggca atctctctcc cctgttaagt aacatctacc tgaacgaatt tgatcaagtg
    22741 ctagaaaatc taggacataa atttgttcga tacgccgatg actgcaatat ctatgttaga
    22801 agcaaacgag caggacagcg tgtgatgaag aaatccattc aatttctcga agaacaacta
    22861 aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc cattgaaacg aaagtttctt
    22921 ggattctgca ttctgccaac aaagaaaggt attaaaattc gccctcacgc aaaagcaaaa
    22981 cagcgtgtga aaagcaagct caaacaactc actaaaagaa accgtggaag aagtatccaa
    23041 cttattttaa aagaaatcaa gcaactaatg actggttgga tcaactacta tggaattggt
    23101 gagatgaaag aattcatgag ggagttaaac ggttggttga agcgaagaat cagacaatat
    23161 atttggaaac aatggaagaa tccaagaacc aaaagaaaaa atctgattcg tttagggatc
    23221 gataaagcga aagcgtatga atggtcaaat acaagaaaag gtatttggtc agtctccaaa
    23281 agtcatattc ttcatcgatc attaacagac aaagaactgg cccgccaagg atatgaggac
    23341 atctccttga agtaccagtt cgtacactca acttattgaa ccgccgtata cgggtccgta
    23401 cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DADVXO010000033        23299 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.034, whole genome shotgun sequence.
ACCESSION   DADVXO010000033 DADVXO010000000
VERSION     DADVXO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.034"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..132)
                     /locus_tag="KB950_001089"
     CDS             complement(<1..132)
                     /locus_tag="KB950_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6857319.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="KB950_001090"
     CDS             542..841
                     /locus_tag="KB950_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6857320.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="KB950_001091"
     CDS             914..2275
                     /locus_tag="KB950_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBB6857321.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="KB950_001092"
     CDS             2297..2557
                     /locus_tag="KB950_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857322.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="KB950_001093"
     CDS             2571..3323
                     /locus_tag="KB950_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBB6857323.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="KB950_001094"
     CDS             complement(3417..4022)
                     /locus_tag="KB950_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6857324.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="KB950_001095"
     CDS             complement(4075..5049)
                     /locus_tag="KB950_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBB6857325.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6146)
                     /locus_tag="KB950_001096"
     CDS             complement(5427..6146)
                     /locus_tag="KB950_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6857326.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="KB950_001097"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="KB950_001097"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBB6857327.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVYEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="KB950_001098"
     CDS             7697..9094
                     /locus_tag="KB950_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6857328.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="KB950_001099"
     CDS             9175..9615
                     /locus_tag="KB950_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6857329.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="KB950_001100"
     CDS             9763..10089
                     /locus_tag="KB950_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6857330.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="KB950_001101"
     CDS             10090..10839
                     /locus_tag="KB950_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857331.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="KB950_001102"
     CDS             complement(10919..11374)
                     /locus_tag="KB950_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBB6857332.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11801..12076)
                     /locus_tag="KB950_001103"
     CDS             complement(11801..12076)
                     /locus_tag="KB950_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857333.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            12370..13185
                     /gene="thiD"
                     /locus_tag="KB950_001104"
     CDS             12370..13185
                     /gene="thiD"
                     /locus_tag="KB950_001104"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBB6857334.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(13514..14965)
                     /gene="cls"
                     /locus_tag="KB950_001105"
     CDS             complement(13514..14965)
                     /gene="cls"
                     /locus_tag="KB950_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBB6857335.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            15306..17972
                     /locus_tag="KB950_001106"
     CDS             15306..17972
                     /locus_tag="KB950_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6857336.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            18336..19088
                     /locus_tag="KB950_001107"
     CDS             18336..19088
                     /locus_tag="KB950_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6857337.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(19110..20264)
                     /locus_tag="KB950_001108"
     CDS             complement(19110..20264)
                     /locus_tag="KB950_001108"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBB6857338.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            20449..22890
                     /locus_tag="KB950_001109"
     CDS             20449..22890
                     /locus_tag="KB950_001109"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBB6857339.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(23047..>23299)
                     /locus_tag="KB950_001110"
     CDS             complement(23047..>23299)
                     /locus_tag="KB950_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6857340.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         7485 a         4090 c         4849 g         6875 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcatat acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaagcaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt
    11761 tattacagaa catttttaat taataaagaa cagttagaag ttaaaattgc tgattcacat
    11821 agagtttatg acttcttgga aattcttttt caatcagttt tttcatctgt ttatgaaatt
    11881 cttttttttc agtaaacata aaggtttggg tatcgtaacc ttcttcatgt aggaaataga
    11941 ttattccttt ttctgtgttt tgcgtataag cttttatact cctgtctttt ttgaaagaac
    12001 agatcgtata gccatctatt tggtctggta gtcgtttgat tttcttcaaa atgacttttt
    12061 tatcctcaag tttcattttt ttcacctgac tttatttttt tcatatggaa atcatagcgc
    12121 ctttggttat atgaaaacaa gaaggatact gaaatatata taaatcaaat agtggttatt
    12181 tattattaga atagcgaata caaataaacg accactattt tctgatttat tgcaaatgtt
    12241 ttttcaaata aatttgatta ccaagaaaag gtagtggtat atttactgag ggatactttg
    12301 aaaaacatat gatagaaaca gtaacagtat tatgaaaaat ctttttataa aaatggagga
    12361 atcgatttga tggaagatca gtatgtacaa gcaatgacga tcgcaggatc agatagcgat
    12421 ggaagcgcag ggatgcaagc agatatgcat acattcttta caagaaatgt ttacggtgtt
    12481 tctgtcatga ccgcatgtgt tgcaggtaat tcttacggaa tcggagcaag tgtgacattg
    12541 ccaacagatt ttatcgacaa agaatttgaa ctgattgcga aggactttca aatacgtgca
    12601 gctaaaacag gtatgctagc agattcaaag ttgattgaga cagttgtaaa aaattaccaa
    12661 aaatatgatt ttggaccatt agtcgtggat cccgtaatcg taacgaaaca tggaaatctt
    12721 ttactagaag aatctgcttt gcaaagcttg aaagaaaagc ttgtcccttt ggccgaagta
    12781 ctgacaccta atttttatga agcagaaaag ctgactggac ggacattagc agctgaagaa
    12841 gattttgtgc aagcagcaaa agaactgcaa gatatgggtg caaaaaacgt tatgatcaaa
    12901 ggacatcatg ctaaaaatgc gcagcaagaa acagtcaatg actacgtatt gttcgaatca
    12961 ggaaaatcat tttggttaaa tgcaccattt tatcctaccg accggatcaa cggaactggc
    13021 gattcgctat ctgcatgtat tgcagctgag ttagcgaaag gaaaaacagt tgaacaagcg
    13081 atccgattgg caaaagacta tgtaaaccgt gcaattcgtg aagaaataaa tgtagggcac
    13141 aaatatggcc ccatcaatca ttgggcagct ggtgaattag agtaaaagaa agaagttgtg
    13201 aaaaagttgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
    13261 taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    13321 cgtgagcctg ccgtccagct ccgagtcaca aggaacaagt tcaaaaaaca gcttctcatg
    13381 tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt ttgttcggaa
    13441 gaagggactg tgacaagatt tttgtcacag tccctttaat cttagtatat ttttctctac
    13501 ttgaataaaa aaattatagg attggagaaa ataatcgaga aatttgttgc ttgaataata
    13561 gccagttaga catttccttg actgtttcag ttgtcatgga tgaacaatgt gtctcatctt
    13621 ctttgaactg agtagcaagt tcttctaaaa actcaggatc gtaaatgaca gcacttgttt
    13681 caaaattcaa tcggtagcta cgaatatctt gatttgctga acctaccatg cagatttcat
    13741 catccattat caatgttttt gcatggagga agccgttctc atagacaagg atttgtacat
    13801 tttccttgat cagctgccga gcataatatt gtgttgctcg ataaataaaa ggatgatccg
    13861 gtttgttggg aatcatgatt ttcacatcta caccagaggc agttgcgatt tttaaagcag
    13921 caatgacact ttcatcagga acaaggtaag gtgtctgtat ccaaacacgt tttttagcag
    13981 aagtaatcaa tttgataaat gaaagcttga tttgttccct ttcgttattc ggtccgcttg
    14041 aaacaagctg gatggatgta tcagcaagct tagattcatc tcgatctgct tttttaaaga
    14101 aataattcaa atgatagccg accttttttt cttcaggtac cgagacgttc cagtctgtta
    14161 agaagcgaag ctggagcaaa gaagaagcgg caccgaaaat ccgtatatgc gtatcgcgcc
    14221 aatagccgaa tttttttgtt gtatttacat attgattggc aatattgaaa ccaccggtat
    14281 aactaatctt tccatcgatc acaacgattt ttcggtgatc atggtaattc aatcggaaac
    14341 gaagtaatgc cctttgtgag gtaacaaatg tatggacatg tccaccattt ttgattagac
    14401 gattgaaatc ttttgctttt gtgccatgag agccaaatgc atcatataat attcgaactt
    14461 ccacgccttc tgcggctttt tcttctaaaa catgtaagac ttgctggccg atattatctg
    14521 tcacaaatgc gtaatattcg atatggatcg aatgtttggc tttttttaga tcttgaagca
    14581 gtgaatccaa tttctcttgt ccgtctgtga gaagagtgac agaattcatt cttgtcagcg
    14641 gcatacgatt taatgaagag aagaagtcaa caaattgctg tttgtcttta tcgcccatgt
    14701 caggatcgta atgttcaatg gtatctcctc taaaagactg aaagttttct aattctttca
    14761 agtcactttg tcggagatag aattttttct tatccgttaa tccacgtccg aaaaataaat
    14821 ataatacaaa gcccacccca ggaagggcaa acaatactaa gagccatgcc caaatggctg
    14881 ctacatctcg gggtttgatc aaaatagcca ccaaagcaat aagtgcattt aatagataaa
    14941 gggcggttat aatactagat accacaaaaa tgttcctcct tacatacaat aacattagta
    15001 tagcatgata gaagaaagaa gcctaccaag gtgaatatac ttttcactcc tttcatgaaa
    15061 atgtaggtaa atcgggctaa agtagttgct ttttgtaggt taataaggta ggatattagt
    15121 acagttgaat atctccttag gggagtaacc ggcaacgttt gttgcgataa tatcaacagt
    15181 aagttaacca aatggttttc tggtattgta tgaaatggtg agacctaaga caaatgcgta
    15241 tttgtcttag gtctcttttt tttcagacag gactagggcc aagatcaaaa aatgggggat
    15301 gaaatttgga aaacaaggaa caagcacaac aagcggtcta taaagaaacg atcgactcgt
    15361 tgatgcagca aacgcagagc tcaaccgatg gattgagcac cacgcaagcg aaggaaaaat
    15421 tagaaagaga tggcttgaac cagctaaaag aaaagccgaa gaagccaact tggaaattgt
    15481 tcttagaaac tttcaaagat gcaatggtta tcgtattgct gattgtcgca ctgattcaga
    15541 tggtcatggg tgactacatc gaattcttag ttatttttgc agtattgatg ttgaattcta
    15601 taatcagtgt tgtccaaaca aaaaaagcag aaggttcttt agaagcgttg aaaaaccttt
    15661 cagcgcccga tgctcgagtg attcgtgatg gtaaggaaaa aacgattccg gcaaacgaac
    15721 ttgtggtagg agatatcgtg ttgttagacg caggcgatta tgtacctgct gatggacgtt
    15781 tactagatgc gggatcatta aaagtagatg aaggtatgct gacaggagag tcgacttctg
    15841 cagaaaaaga agtaacggat atcacaagta cggtaccaat cggagatcgt acaaatatgg
    15901 tattcagtgg aacgatcgta acttacggac gcggtaaatt cttagtgact tcaactggga
    15961 ataatacgga gatcggacaa gttgctggcc ttttggaatc aacaactgag caagtcactc
    16021 cgttacaacg aggtctggat aaattcagta aaaaactaag tttggctatt ttggcattgt
    16081 ctttattgat tttaggtatc caactgtttc gtatttatct tggagaaggt acaggagata
    16141 tgactgcaga tatcgtcagc gcagtgatgt ttgccgtagc cgtagccgta gctgctattc
    16201 cagaagcatt acagtctatc gtaacaatcg tgttatcttt gggaacgaat aaaatggcaa
    16261 aacgtcatgc cattattcgg aagcttccag cagttgaaac acttggttcg acaagtatca
    16321 tctgtacaga taagactggt acattgacac aaaacaaaat gacagttgtt gactactttt
    16381 taggtaatgg cgatactggg gatttcacaa atgacccttc aaaatggaca gctgacgaac
    16441 gtcgcttgat cgaaatcagt gttttagcga atgatgcatc gatcagtgaa gacggtacta
    16501 aattgggcga ccctacggaa gtagcgttta tcgactttag tgaaaaatta aaccagcctt
    16561 atcaggaaat ccgaaacaac tacccaagac aagcagaact tccttttgac tcagatcgaa
    16621 aattaatgtc tactggacac acaattgata acaaaaatta cttgtttgtt aaaggcggac
    16681 cagatatcgt cttccaacgc agtaaaaaag tattgctaaa tggtgaagtc gttccgatga
    16741 cggatgaatt gatgaaaaaa ttccaagagc aaaatgaagc tttttcaaca cgcgcattac
    16801 gtgtactagc ttttgcttac cgtccacttg ataattttga tttatcattt gaagatgaag
    16861 acgatttgat ccttgttggt ttgcttgcta tgatcgatcc tccacgtgct gaagtgactc
    16921 aggcggtaac agaagcaaga agtgcaggaa tcaaaacaat catgattact ggtgaccaca
    16981 aaacaacagc tgttgctatc gcaaaacaaa tcgggatttt tgaagaagga aacctggctt
    17041 tgaccgggac ggaactagat gaattgacag aagatcaact aaacgaagtt ttagaaaaag
    17101 tcactgttta tgcacgtgtt tcacctgaga ataaaattcg tatcgttcgt gcttggcaaa
    17161 ataaaggtca tatctcagct atgacaggtg atggtgtaaa tgacgcacca gcattgaaac
    17221 aagcagacat cggaattgct atgggaacgg gtacagatgt ggcaaaagat gcagcagcga
    17281 tggtattgac ggatgataac tttgcttcta ttgtcagcgc agtagaagta ggacgtaatg
    17341 tgtttgataa tatcaagaaa gctatttctt atctatttgc aggaaattta ggtgcgatca
    17401 ttgcgatcat ctttgcctta gttgctgact ggtccaatcc atttacagca ttacaactgt
    17461 tgttcatcaa tttagtcaat gattcactgc cagcaattgc attaggtaca gaaaaagctg
    17521 aacctgctac gatgaaccgc ccgccaagag atccaaattc aggaatcttt acggacaaaa
    17581 cattgatttc tgtaacgtat cgtggtgtgc tgattgggat tgctgttatc tttgctcaat
    17641 ggcttggcat gcagcattca cctgaattcg gtgtagccat gtcattttca acattgattt
    17701 ggtgtcggac attgcaaaca ttcccagcac gttcaaacac acaaacagct atccaagcag
    17761 ggttcttctc taacagaagt gtcttactag ctgtagttgt atgtagcgcg ctttactgct
    17821 taacaatgat cccaggactg aaagaagtct tctctatccc atcagcattt ggattgacag
    17881 aagtctttac atgtatggga atcgcacttg tgacaattgt tttgatggaa atcactaaat
    17941 tgatcattgt acgagtacaa ggaaataaat aaaacgaatc aacgaggttg tgagcctgcc
    18001 gtttaattct gagtcacaag gtgcaatttc aacaaacagc ttctcatgtt tgttgaatat
    18061 tgtgacttgt gaccgaagag ttggcaggag aacaccgttt aaggaagaag ttgtaaaatc
    18121 agcattttga gctaatactg agggtcatct gattggacac tgtttattcg gaaagaggga
    18181 ctttgacacg actttggtca cagtcccttt ttttatttat gatagacgga aagccaatca
    18241 ttttattatt catcaaaaga gatagaatag ataaaagagg agaatgattt attccgaaaa
    18301 gtgaaaacat ataacaaaaa attagaggaa atcaaatgaa tgaaaaagaa tgggatgaat
    18361 ttgctgaaga atattatgaa atccagcaag aatcgcaagt aactattgct gaagatgtaa
    18421 aaaattatct gaaaacggag aaagtcctgc ctgctgaaac agcagcagac gttgctggag
    18481 gatctggcag atttctttcc ttagcaaaag aaattaatcg ctatgatttg atcgattttt
    18541 cttcaggaat gctagaaaat gcaaaaaaag aagcagaaaa aaatgagttg ccaaatgttt
    18601 cctttatcaa acagtcgtta gaagaattta tccaatcaaa tgagacatat gaaatggttt
    18661 ttacagcagc aaatccagca ttggtacatc ttattcaatt agaaaaattg atgaaaaagt
    18721 caagcaaatg gtgcgtgata atcagagtag tggaatcaat cgatgatgtt ttttatccta
    18781 tcgaaaagca actaggaatg atagaagaag atcctaatac atcgccagtt gtaatggatc
    18841 aatttgaaac atacttaatt gatgaaggat tcaaagtgaa gaaaaaagat tttacttatt
    18901 ttacacagga atcaattact gaagaattga tgaagctata ctatgacgcg tacgcaaaag
    18961 atcctgttgt gaccaggtac attcgcaaac tattcagtga aaaacgagaa atccttagta
    19021 cgactaagct gacctataga ctgctccttt ggtccgcaga taaaaaaaga acctttcacg
    19081 actcgtgaag ggttcttttt atgattttat tatattttgt agtaacgaat atcattttcg
    19141 atctttgtaa tcgaaaaagg tacatctgaa gtatattctg cacattctcc gtgttcagga
    19201 agcgtctcac tgaaaatcgt ttcatattcg tcaacagtga tccgtgttcg agaatctagc
    19261 atctcttgat gagattgtgc gaagagatat tcttgatagt tttcttccag ataacctgta
    19321 aaaaattcac ttactgcacc agaaccgtag ctgaatagac cgatgcgatc tcctggctgt
    19381 agtgatttcg aattttccag tagagaagtt agccccaggt ataatgaacc agtgtaaaga
    19441 ttaccgattc gtcggctgta acggatgctt tcttcatagc gagccataag acgttcctga
    19501 ttttcttcgt ctgtttggtc taatacgctt tgcaatgcct tttttcccat cttagtatac
    19561 ggaatatgga aagcaatcgc ctgataatct tcgagtcctc gacccgacaa ttctttatgt
    19621 cgattccaaa ctttttggaa tgattcgata tacgtagaat tagataaagg accatcaaca
    19681 acaggaaatt cgctataatc tggacgccag aaatcataga tatcttctgt cagaaatacg
    19741 ctgtcgtctt caatcgataa aatacgcggg ttttgagtga tcatcatcgc aacagcaccg
    19801 acaccttgcg tcacttcacc accgcttgcc aagctgtaac gagcaatatc acttgctatg
    19861 actagtactt ttcgttctgg atgatttttg acatattctt tcgccatgtg caaagctgcg
    19921 gtgccaccgt agcaagcctc ttttacttca aaagaacgag caaaagattg gattttcagt
    19981 aaatggtgga taattaccgc actcgctttt gaatgatcaa tcccggattc tgttccgacg
    20041 atgaccatgt cgattagctc acggtctttt tctgttacga ttttgctggc agcatttgct
    20101 cctagtgtaa tgatgtcttc atttgcacga ttgactgcca tttggtcttg accaatccct
    20161 atatggtatt ttgcaggatc atccccacgg ctttctgcca actctgtcat atctaaatat
    20221 aaattaggaa taaaaaagga aagacgatca atccctattt tcatttcaac acctctttac
    20281 ttatgataaa aaacttactt tactaaggta tcataaaaag attaaaagaa atataaaaaa
    20341 gagaatattt aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta
    20401 tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc
    20461 gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt
    20521 actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa
    20581 aaagataaaa tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat
    20641 gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc
    20701 aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta
    20761 ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg
    20821 aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac
    20881 ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca
    20941 caattttctg tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca
    21001 gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat
    21061 gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc
    21121 acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg
    21181 aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg
    21241 ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga
    21301 atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca
    21361 atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa
    21421 ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca
    21481 attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag
    21541 aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta
    21601 gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg
    21661 ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg
    21721 atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg
    21781 agtgaagtgg aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa
    21841 tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa
    21901 atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta
    21961 tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg
    22021 attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat
    22081 atctctactc gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg
    22141 gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac
    22201 aagttacggg agtggttccc tgaagaagag attctattca gtattttaag taattttgct
    22261 acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa
    22321 gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac
    22381 ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca
    22441 gctacaggaa atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat
    22501 ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta
    22561 acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa
    22621 gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca
    22681 gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa
    22741 ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa
    22801 atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata
    22861 caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa
    22921 gatttacagt tattttattt actgcagatc ttgctttttt ggctcttcgt caaattgtgt
    22981 tggcgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat
    23041 atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc gaaagttata
    23101 aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat tcagcccctc
    23161 agtgacgccg tttgagtggg gcaccttaaa ggcatttgaa atacccgatt ggtagcgatg
    23221 gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg ttttaagggt
    23281 ttcttgaaag ccttccgta
//
LOCUS       DADVXO010000034        22750 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.035, whole genome shotgun sequence.
ACCESSION   DADVXO010000034 DADVXO010000000
VERSION     DADVXO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.035"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(122..499)
                     /locus_tag="KB950_001111"
     CDS             complement(122..499)
                     /locus_tag="KB950_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857341.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="KB950_001112"
     CDS             755..1483
                     /locus_tag="KB950_001112"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBB6857342.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="KB950_001113"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="KB950_001113"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBB6857343.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="KB950_001114"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="KB950_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBB6857344.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="KB950_001115"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="KB950_001115"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBB6857345.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="KB950_001116"
     CDS             4618..6057
                     /locus_tag="KB950_001116"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBB6857346.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /locus_tag="KB950_001117"
     CDS             6080..7132
                     /locus_tag="KB950_001117"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBB6857347.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="KB950_001118"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="KB950_001118"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBB6857348.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="KB950_001119"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="KB950_001119"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBB6857349.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="KB950_001120"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="KB950_001120"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBB6857350.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="KB950_001121"
     CDS             10651..11565
                     /locus_tag="KB950_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6857351.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="KB950_001122"
     CDS             complement(11698..13263)
                     /locus_tag="KB950_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6857352.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="KB950_001123"
     CDS             complement(13256..14707)
                     /locus_tag="KB950_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6857353.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="KB950_001124"
     CDS             complement(14721..15737)
                     /locus_tag="KB950_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6857354.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="KB950_001125"
     CDS             15957..17045
                     /locus_tag="KB950_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBB6857355.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="KB950_001126"
     CDS             17099..18625
                     /locus_tag="KB950_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBB6857356.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="KB950_001127"
     CDS             18627..19832
                     /locus_tag="KB950_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6857357.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="KB950_001128"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="KB950_001128"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBB6857358.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..>22750
                     /locus_tag="KB950_001129"
     CDS             21330..>22750
                     /locus_tag="KB950_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBB6857359.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLT"
BASE COUNT         7321 a         3839 c         5155 g         6435 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga
//
LOCUS       DADVXO010000035        22642 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.036, whole genome shotgun sequence.
ACCESSION   DADVXO010000035 DADVXO010000000
VERSION     DADVXO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.036"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            23..3301
                     /locus_tag="KB950_001130"
     CDS             23..3301
                     /locus_tag="KB950_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBB6857360.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3842..5749)
                     /locus_tag="KB950_001131"
     CDS             complement(3842..5749)
                     /locus_tag="KB950_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBB6857361.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5879..6754
                     /locus_tag="KB950_001132"
     CDS             5879..6754
                     /locus_tag="KB950_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBB6857362.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6834..7808
                     /locus_tag="KB950_001133"
     CDS             6834..7808
                     /locus_tag="KB950_001133"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBB6857363.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7988..9133
                     /locus_tag="KB950_001134"
     CDS             7988..9133
                     /locus_tag="KB950_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6857364.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9270..9614
                     /locus_tag="KB950_001135"
     CDS             9270..9614
                     /locus_tag="KB950_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBB6857365.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9885..11171
                     /gene="efmA"
                     /locus_tag="KB950_001136"
     CDS             9885..11171
                     /gene="efmA"
                     /locus_tag="KB950_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBB6857366.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11309..11908)
                     /locus_tag="KB950_001137"
     CDS             complement(11309..11908)
                     /locus_tag="KB950_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBB6857367.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12312..13433
                     /gene="mnmA"
                     /locus_tag="KB950_001138"
     CDS             12312..13433
                     /gene="mnmA"
                     /locus_tag="KB950_001138"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBB6857368.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13679..15100
                     /gene="arcD"
                     /locus_tag="KB950_001139"
     CDS             13679..15100
                     /gene="arcD"
                     /locus_tag="KB950_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBB6857369.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15114..16232
                     /locus_tag="KB950_001140"
     CDS             15114..16232
                     /locus_tag="KB950_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBB6857370.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16292..17584)
                     /locus_tag="KB950_001141"
     CDS             complement(16292..17584)
                     /locus_tag="KB950_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6857371.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17716..18294
                     /locus_tag="KB950_001142"
     CDS             17716..18294
                     /locus_tag="KB950_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6857372.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18423..18800
                     /locus_tag="KB950_001143"
     CDS             18423..18800
                     /locus_tag="KB950_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBB6857373.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18733..20805
                     /locus_tag="KB950_001144"
     CDS             18733..20805
                     /locus_tag="KB950_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6857374.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21016..21963)
                     /locus_tag="KB950_001145"
     CDS             complement(21016..21963)
                     /locus_tag="KB950_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6857375.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22209..>22642
                     /locus_tag="KB950_001146"
     CDS             22209..>22642
                     /locus_tag="KB950_001146"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBB6857376.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7328 a         4137 c         4808 g         6369 t
ORIGIN      
        1 atttgttacg ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat
       61 gaatctcctt atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga
      121 acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga
      181 ggaaatacca agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa
      241 agaatcagaa aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg
      301 ggttgttttc ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg
      361 cgtgcggctc tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat
      421 gacctattat tatcaagcaa tccctaaaga catcaacttt aggatgcggg cgacgatcga
      481 aatagaatct tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc
      541 agtaccaaaa aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg
      601 cagtcggatc gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag
      661 caaatatacg atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga
      721 tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc
      781 agcagggttc ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa
      841 tctgtttccg gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac
      901 tggtttagag gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat
      961 gtatgactgg gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc
     1021 agcaaggaat atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac
     1081 agatcctcct gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc
     1141 tagtaatcat tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt
     1201 attgacaatt aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat
     1261 cactgcgaat cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac
     1321 tttcagtttt acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta
     1381 tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta
     1441 tgtaacacca gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac
     1501 acaagcattg agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc
     1561 cttcgaaaga ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca
     1621 gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg
     1681 aaatgggatg acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag
     1741 tgcagctatg caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca
     1801 tgcttatcgg aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga
     1861 agcatggccc gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg
     1921 atttgaagat gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga
     1981 cggttgtatc gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac
     2041 aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc
     2101 aggaattgag ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg
     2161 ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa
     2221 tagcggaaca gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt
     2281 ggctctttat acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt
     2341 ccgaacagag aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca
     2401 aatctaccac tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct
     2461 tggacatacc atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca
     2521 gcagcctgtt acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa
     2581 accggggaat cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc
     2641 tatttttgac ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga
     2701 accaaatcag acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg
     2761 tgtggcggag aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc
     2821 aactttcaca ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa
     2881 gatccaaatc gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc
     2941 agcaccttca aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg
     3001 ggaaaaatgg tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga
     3061 aactgccgat gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc
     3121 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3181 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3241 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3301 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3361 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3421 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3481 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa
     3541 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3601 taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3661 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta
     3721 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3781 agaagcgatt ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     3841 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     3901 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     3961 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4021 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4081 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4141 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4201 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4261 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4321 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4381 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4441 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg
     4501 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4561 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4621 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4681 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4741 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4801 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     4861 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     4921 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     4981 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5041 ccgtgacaag ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt
     5101 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5161 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5221 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5281 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5341 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5401 gtaatgtatc caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg
     5461 attcaacttt ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat
     5521 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5581 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5641 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5701 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5761 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     5821 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     5881 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     5941 agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt
     6001 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6061 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6121 gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat
     6181 tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt
     6241 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6301 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct
     6361 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6421 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6481 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6541 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6601 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6661 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6721 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6781 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     6841 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     6901 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     6961 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7021 aatcaacgaa tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa
     7081 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7141 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7201 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7261 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7321 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7381 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7441 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7501 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7561 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7621 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7681 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg
     7741 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7801 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc
     7861 tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     7921 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     7981 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8041 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8101 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8161 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8221 taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac
     8281 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8341 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8401 agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa
     8461 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8521 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8581 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8641 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8701 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8761 tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     8821 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     8881 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     8941 taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca
     9001 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9061 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc
     9121 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9181 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9241 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9301 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9361 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9421 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9481 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9541 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9601 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9661 gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca
     9721 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg
     9781 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
     9841 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
     9901 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
     9961 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10021 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10081 gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10141 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10201 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10261 tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga
    10321 atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg
    10381 ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt
    10441 ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag
    10501 ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag
    10561 ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca
    10621 gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag
    10681 tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa
    10741 catggaaaga ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg
    10801 gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg
    10861 caggagcagg ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga
    10921 gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg
    10981 caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa
    11041 tgttcttgtt ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag
    11101 ctgcccgaaa ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa
    11161 aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc
    11221 ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt
    11281 gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat
    11341 agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga
    11401 gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat
    11461 gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat
    11521 gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa
    11581 atttgggact tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc
    11641 gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca
    11701 aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa
    11761 catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat
    11821 ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa
    11881 agtgcatatc actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa
    11941 acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg
    12001 tgcagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta
    12061 ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc
    12121 tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag
    12181 aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat
    12241 gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg
    12301 atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg
    12361 tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat
    12421 tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata
    12481 aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa
    12541 aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac
    12601 ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa
    12661 tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa
    12721 acggcgtggt ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta
    12781 gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat
    12841 cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa
    12901 cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag
    12961 caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga
    13021 tgtattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag
    13081 aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt
    13141 tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga
    13201 atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg
    13261 ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg
    13321 tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg
    13381 gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat
    13441 taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag
    13501 ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa
    13561 agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa
    13621 aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat
    13681 ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc
    13741 aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc
    13801 agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa
    13861 tcatttagga gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc
    13921 atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg
    13981 aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa
    14041 aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt
    14101 attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa
    14161 attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt
    14221 cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg
    14281 aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat
    14341 cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc
    14401 gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg
    14461 cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa
    14521 aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat
    14581 tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga
    14641 cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt
    14701 agttgttact gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc
    14761 ctatcaattt gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg
    14821 gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg
    14881 gattgctgta gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt
    14941 atgtagtatt gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa
    15001 acatcaaatt acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct
    15061 ttcaatcggg ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa
    15121 tcgcaaattt cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata
    15181 ttgcacaaag ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta
    15241 gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag
    15301 gatcagatgt tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca
    15361 tcttacaaac gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag
    15421 caggagacca tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat
    15481 ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt
    15541 tagaacagct agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa
    15601 acaatcctac aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc
    15661 gtacagtaga tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg
    15721 aattcttgtc gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa
    15781 cctactccat tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag
    15841 tttttcgaaa gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg
    15901 ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag
    15961 agaaccttgt gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc
    16021 ccaattacgt ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt
    16081 gtatcaattt attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac
    16141 cgaagcatgc ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc
    16201 tcttatctga gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga
    16261 caactgttca agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg
    16321 aagctatgga gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg
    16381 tattgtgtcg tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc
    16441 cgcgataacc aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag
    16501 ttgcttggaa agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt
    16561 ttgtgatcaa tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac
    16621 gtaaggcagc taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg
    16681 caaaacccaa accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa
    16741 atcttggagc ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca
    16801 acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa
    16861 tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg
    16921 gatatttcaa gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga
    16981 caaatttttc atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg
    17041 taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc
    17101 caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt
    17161 cttttggcgt cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc
    17221 cttgaagcag acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa
    17281 tgacgattgc cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg
    17341 ctaattttcc ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca
    17401 atccgctcca aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg
    17461 acataaaagg catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga
    17521 caaacaagtt ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag
    17581 ccaattagac tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat
    17641 atgtagttta tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag
    17701 tgaaggattt attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag
    17761 aaaatcatcg actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt
    17821 cagcatttag ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat
    17881 attcgtgatg tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg
    17941 cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat
    18001 cgatcctttg ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc
    18061 aatgaagctg ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt
    18121 gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg
    18181 atccacggtt atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat
    18241 ccagaagaca gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc
    18301 agattcattt gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc
    18361 ttgcaacttt cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga
    18421 gtatgtatca agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact
    18481 gggaagaaga tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt
    18541 acgaaaaaca atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact
    18601 ttctcagcgc cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt
    18661 tacagcaata caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca
    18721 gaaaggaatt ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta
    18781 agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc
    18841 gttacttggg cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa
    18901 tggcagagct ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca
    18961 cttcctaaaa caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc
    19021 tcagaagcag tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc
    19081 ttagagtatg tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat
    19141 aaagcaatca aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat
    19201 gaatctgctc ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc
    19261 ttaactgtga agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca
    19321 ttagtgagag accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc
    19381 acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa
    19441 acacaagaag aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca
    19501 gaagtccaag aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc
    19561 caaagagaag ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa
    19621 agtttgtatg agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca
    19681 aatgacatga aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag
    19741 gtcaagcagt atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt
    19801 aaagtgaccg atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta
    19861 agtaatgaag agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct
    19921 caaccccata aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt
    19981 tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa
    20041 acagtcaaat gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta
    20101 tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt
    20161 ctgggaaccc cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg
    20221 gaacgtaccc ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta
    20281 aatccctctt tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt
    20341 gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc
    20401 ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag
    20461 aaatgcccag aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat
    20521 gtctgcacca attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat
    20581 cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc
    20641 cgttgtaaat acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa
    20701 atgaccaaac atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa
    20761 gaaagcccat tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag
    20821 gttgtgatag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca
    20881 aatttttgtg aaatttcggc ttatttccga agaagctact tctggaacac catttattcg
    20941 gaaaaaagga ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac
    21001 ttcatttgac gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc
    21061 tcttatgata taaacaggac gctgtttcgt ttctaagaag attttcccaa tatatttccc
    21121 gatgattcca agacataaca attggattcc accaatgaac aggaagatcg agaccatgga
    21181 aggccatcct gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc
    21241 aataccagaa ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc
    21301 cgagaagttc acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt
    21361 ttcgccagcc acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca
    21421 gctaaataat ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat
    21481 ttgacgagtc atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt
    21541 attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg
    21601 agtcgaacgc cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc
    21661 aggaagcagt tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt
    21721 agaagcttgc aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac
    21781 aaagcggaca taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt
    21841 tgatccgtcg tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc
    21901 atcaaaaaaa agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt
    21961 catcgtttct atcctttcaa tcgtatgttt atatatggtt tacattcaaa atttaataaa
    22021 ccttaaataa cacctttaaa atttgatttt acgtaaaaga acaggtattc tttttgtaaa
    22081 ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa cttttaagaa caatttttat
    22141 tagttggcaa gtagttttgg atagagaaaa aggaggaagt tccagtctat tgactaggag
    22201 caaacaaaat gaaaatacac acacctatcg gctttttatg gttagatgca gatgaatccg
    22261 gattgactgc tgtatctttt cagccaattt cagaaagcga atcagcgaat cagcgaattt
    22321 taacgcgcac acaagaacag tttgaagaat attttgcggg aaaaagaaaa gaatttgatg
    22381 ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga
    22441 ccattcctta tggagaaatc cgaacgtata aagaagtagc cgttgctgtt gatagtccga
    22501 aagctgtgcg agcgattggg caggcaaatc gaaccaatcc attgccaatc gttattcctt
    22561 gtcatcgagt gatcgggcaa aatggaaaac tgacaggata tatgggaaac gcaatagatg
    22621 gggttgaaat caaacgccaa tt
//
LOCUS       DADVXO010000036        22386 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.037, whole genome shotgun sequence.
ACCESSION   DADVXO010000036 DADVXO010000000
VERSION     DADVXO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.037"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(135..587)
                     /locus_tag="KB950_001147"
     CDS             complement(135..587)
                     /locus_tag="KB950_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBB6857377.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(600..1010)
                     /locus_tag="KB950_001148"
     CDS             complement(600..1010)
                     /locus_tag="KB950_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6857378.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1285..1497
                     /locus_tag="KB950_001149"
     CDS             1285..1497
                     /locus_tag="KB950_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6857379.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1500..1712
                     /locus_tag="KB950_001150"
     CDS             1500..1712
                     /locus_tag="KB950_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857380.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1852..2007
                     /locus_tag="KB950_001151"
     CDS             1852..2007
                     /locus_tag="KB950_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857381.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2004..2228)
                     /locus_tag="KB950_001152"
     CDS             complement(2004..2228)
                     /locus_tag="KB950_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBB6857382.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2306..2494
                     /locus_tag="KB950_001153"
     CDS             2306..2494
                     /locus_tag="KB950_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857383.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2820..3155
                     /locus_tag="KB950_001154"
     CDS             2820..3155
                     /locus_tag="KB950_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857384.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3388..4329
                     /locus_tag="KB950_001155"
     CDS             3388..4329
                     /locus_tag="KB950_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBB6857385.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4331..5221
                     /locus_tag="KB950_001156"
     CDS             4331..5221
                     /locus_tag="KB950_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBB6857386.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5264..6064
                     /locus_tag="KB950_001157"
     CDS             5264..6064
                     /locus_tag="KB950_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBB6857387.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6079..6930
                     /locus_tag="KB950_001158"
     CDS             6079..6930
                     /locus_tag="KB950_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6857388.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7131..7592
                     /locus_tag="KB950_001159"
     CDS             7131..7592
                     /locus_tag="KB950_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6857389.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7618..8070
                     /locus_tag="KB950_001160"
     CDS             7618..8070
                     /locus_tag="KB950_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857390.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8176..8370
                     /locus_tag="KB950_001161"
     CDS             8176..8370
                     /locus_tag="KB950_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857391.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8401..8787
                     /locus_tag="KB950_001162"
     CDS             8401..8787
                     /locus_tag="KB950_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857392.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8784..8987
                     /locus_tag="KB950_001163"
     CDS             8784..8987
                     /locus_tag="KB950_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBB6857393.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8984..9280
                     /locus_tag="KB950_001164"
     CDS             8984..9280
                     /locus_tag="KB950_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857394.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9356..9772
                     /locus_tag="KB950_001165"
     CDS             9356..9772
                     /locus_tag="KB950_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBB6857395.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10033..10170
                     /locus_tag="KB950_001166"
     CDS             10033..10170
                     /locus_tag="KB950_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857396.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10580..10810
                     /locus_tag="KB950_001167"
     CDS             10580..10810
                     /locus_tag="KB950_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857397.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            10969..11802
                     /locus_tag="KB950_001168"
     CDS             10969..11802
                     /locus_tag="KB950_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBB6857398.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11795..13210
                     /locus_tag="KB950_001169"
     CDS             11795..13210
                     /locus_tag="KB950_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBB6857399.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13222..14751
                     /locus_tag="KB950_001170"
     CDS             13222..14751
                     /locus_tag="KB950_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6857400.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14669..15721
                     /locus_tag="KB950_001171"
     CDS             14669..15721
                     /locus_tag="KB950_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBB6857401.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15723..16040
                     /locus_tag="KB950_001172"
     CDS             15723..16040
                     /locus_tag="KB950_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857402.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16167..16790
                     /locus_tag="KB950_001173"
     CDS             16167..16790
                     /locus_tag="KB950_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBB6857403.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16805..17695
                     /locus_tag="KB950_001174"
     CDS             16805..17695
                     /locus_tag="KB950_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBB6857404.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17718..17933
                     /locus_tag="KB950_001175"
     CDS             17718..17933
                     /locus_tag="KB950_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857405.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17945..18286
                     /locus_tag="KB950_001176"
     CDS             17945..18286
                     /locus_tag="KB950_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857406.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18286..18657
                     /locus_tag="KB950_001177"
     CDS             18286..18657
                     /locus_tag="KB950_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857407.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18650..19045
                     /locus_tag="KB950_001178"
     CDS             18650..19045
                     /locus_tag="KB950_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBB6857408.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19047..19424
                     /locus_tag="KB950_001179"
     CDS             19047..19424
                     /locus_tag="KB950_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857409.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19425..20033
                     /locus_tag="KB950_001180"
     CDS             19425..20033
                     /locus_tag="KB950_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6857410.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20033..20374
                     /locus_tag="KB950_001181"
     CDS             20033..20374
                     /locus_tag="KB950_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857411.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20616..>22386
                     /locus_tag="KB950_001182"
     CDS             20616..>22386
                     /locus_tag="KB950_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBB6857412.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIA"
BASE COUNT         8103 a         3272 c         4632 g         6379 t
ORIGIN      
        1 gtgattccta taattaataa aataagtcca atcaataata acattcaaaa ctcctcattt
       61 ctttgatata attattgtag agtttactca gaaatgagga aagagtccat gtgaaagcat
      121 gggctttttt atttttaatg ttgtaatact aaatgttcaa gattatgagg aagccctaaa
      181 tattcaataa tttggaactg actcatatca gataaatctt gttcatctat tgttctagtt
      241 aacatttcaa ttgcaaacat attagcttca gcttctatac cataaatcat acaactaagg
      301 ttgtttgctc taaaagctgc tgtgcaaata cctttatgga gtctaacgtg ggctatttca
      361 tgccataata caaatctttt catagaaggt gataggtttt gattaattaa tatcacataa
      421 catcttttat tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt ttcaattaaa
      481 tctatatcag aatatttgat tattttatat ggattaatcg catcagatcc aaactctaca
      541 aataattttt ctatttcttc taatatccag tatttgttta aatgcatacc aaagcccacc
      601 tattgacgat attttttagg agtgaatttt ttctttgctt taactttggt taaggcaagt
      661 gcaagttgaa tggcatcgta taatttttcc ttatcttcat cgtctaaagg ttcaccataa
      721 taatttgttt cagcatctga attcaagcct tcaagtaatt tttcagcttg taaagcaata
      781 tcttgtttat ctttttctgt taaatcataa taatggcgtt tatcagtacg tcctaaaaga
      841 taatcagttg atacattaaa gtaatctgca actttttgta ttttatctcc agatggagta
      901 cttttattcc atctagacat gcttccattt ccgaaattta actttctttc aagttctgcc
      961 aacgtcattg aacgttcttt taataagatt tttatacggt ctaatagcat aggatatgca
     1021 ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa attatctatt tacaaataga
     1081 gaatttgcag ttatactagt tttgtaagca agatatttac ggcatacaaa gactaaaagc
     1141 aataaactaa tagtctcccc agacatcagt aagctcgctt tttgtgcgct gtattttgta
     1201 tgcttacata atagcatatt tgcagttttt gtcaaactta ttagacaaaa atattagaaa
     1261 atattcgata agggggtatg aagcatgact ttaaaacagc aagtattaat tgctttagta
     1321 aagaaaggtt ggagtcaaag agagttagct cgacgaatgg gtatatcaat tacttattta
     1381 cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat cgaagaactt
     1441 ttggatatca agttagaagt agattctaat gatcagccat cagaacagga ggcgtaaaaa
     1501 tggtacaacg tttgagcaaa gcggagttta aagagaaata taagtattct gaaagtactt
     1561 atcaaagaag aatgaaggaa tttaaaaaat cacgttttag tgaggggtat aacgcagtta
     1621 cttccaaaga aataacaatt gatgtcgatt tatatgaaca gtttagaaca tggcgttcta
     1681 aaaatcgatt tagagttaga gctgtttgtt aatagctaaa aaatctaaat tcaccacttc
     1741 tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa gtccacgttt tttcattttg
     1801 gcaactcctc ttcttggagg tggtgacggc gagagacgaa ggagggatga catgaaggaa
     1861 gaatcaaaaa tagggatttt gaatgagaac aattacacaa gattaattat tgaaacagaa
     1921 gaagggaaaa aagtagcaga agttacattg accgatgcaa ctcctgctga cggttatcgt
     1981 attcgattaa ctcctaaata tgattaacct tttggtgggt gtggatcgtt tccgtgacta
     2041 tcacggcttt gaatttttcc atctctgcca tgaataaata gctcagatcc ttgattttta
     2101 gaaatctctc tagcgatttt tactgcttct gattttgtat tagtatgtgc agtcgcttta
     2161 gaatttccag cacctttgac attccaacca ccattggaat taggtacaac atgttgattt
     2221 tttcccatag ttttcacctc catttctata gaaatatttt atcaaataga aatggagaaa
     2281 ttcaataaac gaaaggagaa gtgatatggc atataccttg caacaagaac atcaaattct
     2341 cggtttgatt aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc ttagaaaagc
     2401 cgatgagcta tcagataggc aagctgaact aattgcttct gaacttgagg atttgagaat
     2461 gctagaaata aaaaataggg agattagatt atgaagaaga cagacacact ttttatagga
     2521 ttcattttgg ggttattagt gattgtagcg caccaaagta ttatcggggg aagcttgttc
     2581 gcagcattga tggttttaat caatctgctt gattcaaaag aaaggagcaa ctatggcacg
     2641 agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa atagccgccc
     2701 tattatttta agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg
     2761 actctgccag ccagcaaaga gtcacataca aatttataaa actaggagaa ttatatcaca
     2821 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
     2881 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
     2941 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
     3001 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
     3061 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
     3121 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
     3181 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
     3241 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
     3301 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
     3361 aagcaccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
     3421 gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac aattttagga
     3481 ttgaataaat ggaaatctcc ttatcaacta tggctagaaa aaacaggaca agtagtactt
     3541 gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt ggttgctaaa
     3601 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
     3661 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
     3721 gaatgcaaaa cagctaatca atttctcggt aaagaatggg aaggcgaaga ggtaccgctt
     3781 agctatctct gtcaagttca acattacatg aacgttctaa acaaagacta ttgttacatc
     3841 gctgtcttaa tcggtggtca aaaatttatc tggaagcgga ttgaacgaga tcaagagctg
     3901 atcgatacaa tcactgaaca attagtagag ttttgggaaa caaacgttct tggaggtatc
     3961 gagccaatta ttgatggtag tcaagcgaca gctgatttct tgaaagagaa gtatgcagat
     4021 gttgaagacg ttcaaacgac gttaccaatt catttcgatg aactagttga acagaaaaac
     4081 gaactcaaac ggactaagaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt
     4141 atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt
     4201 tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat
     4261 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaagggag
     4321 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa agccacagaa
     4381 acaagttgca ccaggtcaat taggacttaa agctttgatg aatacaccaa caatgagaaa
     4441 gaagttcgaa gaggtgcttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
     4501 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
     4561 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
     4621 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
     4681 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
     4741 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
     4801 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
     4861 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
     4921 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
     4981 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
     5041 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
     5101 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
     5161 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
     5221 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
     5281 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
     5341 atggaacttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
     5401 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
     5461 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
     5521 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
     5581 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
     5641 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
     5701 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
     5761 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
     5821 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
     5881 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
     5941 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcca atcgtgatta
     6001 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
     6061 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
     6121 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
     6181 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
     6241 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
     6301 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
     6361 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
     6421 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
     6481 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
     6541 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
     6601 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
     6661 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
     6721 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
     6781 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
     6841 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
     6901 ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc
     6961 gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga
     7021 gtcgaatggt tcggaagata agtaagctaa ttatttatcg ggatacagaa agatttctca
     7081 ctgttttagg taacgtctac gagaatccag agttattgga ggtagagtaa ttgccaaaaa
     7141 tattagatgc atgttgcggt agtagattgt tctggtttga taagaacaat tctgacgtaa
     7201 catttatgga ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg atcaatgttg
     7261 atccagatgt ggtcgcagac tttcgcgata tgccttttga agatgatgtg ttcgatatgg
     7321 ttgtatttga cccgccacac ttaagacatg tcggcgaaaa ctcatggctt gccaaaaaat
     7381 atggaaaatt agacgaactt tggccagaag acatcaggca aggttttgca gagtgtatgc
     7441 gagttttacg accttcagga acattaattt ttaagtggaa cgaggaacag ataccacttt
     7501 ctgatgtgtt agaggctatt ggagaacagc cattgtttgg taataaacga agcaaaacac
     7561 attggcttgt gtttatgaaa aaattcagct aacggaggaa acaaaggagg aaaagtaatg
     7621 aagattgtat taaataaatg ttttggtggc tttgggttga gccatgaagc aaaaatggaa
     7681 atttttaaaa gaaaaaatat tgaagtattc ccatacatca ataactttag ctatgactca
     7741 gatgatgaat acacgagata cacaggccaa aagctaggtt cgatggattt tatctactat
     7801 ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt atagcgaaat tgaccgactg
     7861 tacggaattg ctgatgattc cagtttttca agtgattcaa acagaggtga taaggacctc
     7921 gttgctgttg tcgaaaaact aggaagtgag gccagtggac catatgcaag tcttaaagtc
     7981 gttgatatac ccgatggggc tgagtgggaa ataagtgatt atgacggcgt tgaaactgca
     8041 cattatggtt ttcaaacagg tagtatttag tccactaatg acaactaaag aggaggcaga
     8101 aggatgagtg aagatgaata ctttgaacag tagtctatta acgacagaga tttttaactg
     8161 gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata aaattaatag tctcaaagag
     8221 gttttcggag acgtcgaagt agtgataaac gcacaaggct atgacaacgg attttttaag
     8281 cagattaccg acattagcat tcagcaagga atggaagacg aagatggaaa ctttatcgat
     8341 gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg aggcagaagg
     8401 atgaaaaaat tgcaccaact catatctgag aaagaaagcg aactccaaaa tttagaagat
     8461 agtttgggat tggggtttcc aattgttgag caagctaaaa tgactcaaat cagtcatcta
     8521 cgacttgaat tggaagattt aagacaaata gaaaaatcaa ttcaactcaa cgacaaccag
     8581 aaaatcgtgt tggagtggtt gaaaagcgag acaattttaa ctagagaagc accaatatta
     8641 tctgttaatg ctttttctga taaaaattta ctaggaaaat tacctgataa agtacgcaaa
     8701 gcttataaac tattggcttg taaacaagaa tatgaagtcc tatcagcatt cgctcaatgg
     8761 ggattagaac aggaggaagc ggaatgaaca acagacatcg cagaataaca aaactaagaa
     8821 aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag
     8881 aaacatataa agtggcaagt cagtttgttg ctgatgcgag taatgctatt cgtaagtttg
     8941 ggatttcgat attaaatgat gatcgtaaat gggaggaaat gagatgaaaa taaaagacgg
     9001 attttacgct agtagtcatg gtatcggcgg tttaatgcta gacatgccga taaagaaccc
     9061 taaaacacgt cagaaatcaa aattcaaaat cggtgacatg gtccgatgta aagcagaaga
     9121 gttcatctat ccgtttcgtg gatatgtaga gcatgtctat aatcattcag caatcattca
     9181 cattgaaaac acgatggaat gcgataagtg gttagcgaaa agcaaagata atttagcagt
     9241 ggctcgtttg gtggatatgg aactaatcaa tgacaaataa aaaagccgga tcgctccgac
     9301 tgattcaata aatccaacac atttattata tcacataaag gagcggtttg acttgatgca
     9361 attgttacga gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa
     9421 ctttcggcgt ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat
     9481 tacggatatg ccgaaggcac cgaagcacgg caataaggca gaagacgcga tcattcagat
     9541 gatggatata gaagcggaga gagacgcaat actagcggct ttgatggctc ttagtctgat
     9601 tagccgtcag atactctact acagcttctg tgacgtaaac aagcactcta attatgaaat
     9661 agggcaattg atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt
     9721 aatcgaattt gcagaagcat acaaaaaagg cgtgttagtt cagtatcgtt gattttgtag
     9781 ggtttttgta gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc
     9841 gaaagattag tgataggtct gagacaaaat aaaatgtaag ggaggaaatc tccctcatcg
     9901 ttttaactta agcttcgata gacagcaacg gaaatattaa gaataaggat gtgaatttca
     9961 actccttcta aattgttctt attatctatc atccgttgtt gtctattaat ttatgcatcg
    10021 gaggaaaaac gaatggataa agaaatcaaa gtaactgtca aattagattt gactgaacta
    10081 aaagaactgc tcaacaaggc tagtgaccaa gtcgaacagt tacaagaaac tttagatgaa
    10141 attgctaatt ttaaaatcca agtttcttag cagtatattt tttagcagct gcactcataa
    10201 aatcatacca agtatcagaa aaatataact taactaaata attaaaaaag gtgggtaaat
    10261 agaaatgacg gcgttaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg
    10321 atttaaaaat ggacaaaata aaaaatagta gcagccgaga ataattttat agtgtcactg
    10381 tggcggaaat agaagacgca aaggtggagg taggaagcgt atggcgcact atcgggacct
    10441 tgtacaaaac tagaaaggac tagtacgtgt gtggtgcgag ccctagggag gaacaggata
    10501 accgcctgtg tgtaggttgc tattacatat tagatcactc tttgagtggt ctttttattt
    10561 acataaagga ggctacataa tgagaaacta ctggtatata tcgctaacta atgaatatcc
    10621 tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct gtccaaatca aaaagaagta
    10681 ctccattgtt gaaatgacca gagaagctac gccaaaagag attgataaat gcaaacttct
    10741 ttattgcggt catggtttct atgatgaacc aaacattcaa aataatatta acaagaattt
    10801 gagggattag aatgcaagaa atggcttaaa aatctccaat caggacaaat caagttagac
    10861 aaagtttccg atttaaagat attgattgaa gctgatttaa tgctaaaaga tattgaaaat
    10921 tagaaaacaa aactcaacct aagaagattg cgaggtggtg tgtattgaat ggcaagacaa
    10981 cgtgatccaa gacgtgatga agccaaaaga atttggttag aatccaacgg agaaaagcag
    11041 ttaaaggaaa ttgcatctga attaaatgtt tcagattctc aggttagaaa atggaaatcg
    11101 caagacaaat ggagcgctga attgaaaagt aacgttacca atggcaaaag taacgttact
    11161 aatcaaggtg gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg aaacagcaga
    11221 gcatcaccgc caaagagaaa taagaatgct cttaaaactg gtgagtatga aacaatattt
    11281 tttgacacgt taaccgataa tgagaaggac atctattcta gtttgaatga tgatccttct
    11341 tttgctttgt ccgaagaaat acgacttctt aaaataagac aacttcgaat gatgaaaaga
    11401 atccaacaag ctgaagctgg actaaatgat gaagaagtcg aacgattgca gcagctaaga
    11461 aagattaaaa ctccgattga aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg
    11521 caagatgtgc agattagcag aaaaaaacat cgcaaaattg atgatattct ttcaattgaa
    11581 gattcattga ctcggattag caaccagtta gctaaagcca tcaagcaaat gaatgaactt
    11641 tatatgaatg aatacagaac tgatttaatt aaagctcaga ctgataagat ccaagctgag
    11701 acaaatgaaa ttggcggaaa taattcaggt gaagaaatag aagaatggaa acaggcagtt
    11761 ttaaatgccg caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc
    11821 tgatattggt tctgccattg attattacta tgataaacca gtagcttttt gccaagatat
    11881 tttgcatttg aatcctgatg aatggcaaga aaatgtttta aatgatttag ctgaattttc
    11941 aaaggtttct gttcgttctg gtcaaggagt tggaaaaaca gcattagaag caggagcaat
    12001 actttggttc ttaacgtgtc gaccctacgc taaagtaata gcaacagctc cgacaatgaa
    12061 gcaactttac gatgtacttt gggcagaggt agctaaatgg ttaaatgata gcttgatcaa
    12121 aaacttactg aagtggacaa agaccaaaat ttatatggtt ggtgattcag agcgttggtt
    12181 tgctacggct agaacagcga ctaaaccaga aaatatgcaa ggttttcacg aggaccatat
    12241 gttgattgtg gtagatgaag cttctggtgt gtctgatcca attatggaag ctattcttgg
    12301 tacgctatca ggttttgata ataagctgtt gatgtgtgga aaccctaata atattgaagg
    12361 tgtcttttac gattcccaca attcagaccg tgataaatac agagttcata aagtatcaag
    12421 ctatgatagt aaacgtacaa acaaagacaa tatagaaatg attcttaaaa aatatggaaa
    12481 agaaagtgat gttgctcgtg tccgtatttt tggagaattt cccaaaggtg cgttggattc
    12541 atttatcagt cttgaaacgg ttgaattggc tacagaaaaa caaattagtg attctttagt
    12601 caataaaaca acggttgctc atattggtgt tgacgtagct cgatatggtg atgattctac
    12661 gattctcttt cctagaattg ctaccagggc attggagtat gagaagtatt caaaacgtag
    12721 cactatggaa acaacaggat atgtcatcaa catggccaag aatctaatga gtcaatatcc
    12781 gagtattgat aaagtgatga ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga
    12841 ccgcctagaa gaacttatag aagacaagca ttatcctttt gaggtgtttg gagtgaataa
    12901 cggttcaaca tcagaagacg atttttacga taatttaggt actcaactat ggggaaacat
    12961 caaggaaatg ttagaagaaa atatgacagc aaatcttaac ggagaacagc ctgttattga
    13021 attgccttct gatagttcgt taatcaaaga attaagtact cgcaaattca aaatgacaag
    13081 tagaagtcgt atacgtttag aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc
    13141 cgatattgct gacgcactgg ctttagcgtt ttatgagcca ccaagtcact atcaatttat
    13201 tcaattttag gaggtgagtt tttgattagt acagtattat ctttagagag atataagaag
    13261 ctacgtgtta aatatgcaac acaaattgaa gatggaatgt ttgatccgaa tggttttata
    13321 gaatatatga aaccattttt tgctgatcgc gaaagaaaat atctagctta tactagcgaa
    13381 aagaatgaaa tagacaatag accaaagcct aacactgata ttgtaaaagt taataataaa
    13441 cttcatgctg gtatgtattc aattgtcgta gaccaagcag tcaatcattt tactggtata
    13501 cctatcaaat gggattacga tgtatcggaa caaaaaagaa cactcattca aagattaaaa
    13561 gataaattct taaaaaatga tatagaactt ccaacagttc cagaagcttt taacaaatta
    13621 acaagcaatc ttgattccat gagattcgca atgcttgatt ctgaaactgc aacttttcaa
    13681 ggagcttgtg gagtagcttt ccgattgtta gagcctgtgg aagaagatga tggttggaag
    13741 ttaagagcaa gtaatattga accttggaga gcagaaagat atggaaatgc cggaatctat
    13801 atcaaagaaa agtatgactc ttaccagaaa aaattttttc aagaaatgaa agttatgaca
    13861 agaaataaaa ttctgacata tgcttgctat ggtgatttga attttgttac aagcggaacg
    13921 ttcaaaaaaa ttgatgagac cgataaccct ttggggacga ttaccttggc agaatttaaa
    13981 aataatacga atcgttattg tgattttgaa gtagctgagg aaattggaga tgcaattgat
    14041 cgtgctttgt ctgatcaaca aaacgaaatc gaacaattta agcttgcgta catgcttgtt
    14101 actggcacaa caatgagcaa aggcactgct aaagaaatga tgaatcagct agggattatc
    14161 aacttaaaag accctactgc aaaagctgaa tatgtcacaa aaaaattagc aaaagatttc
    14221 aatgaatatc atatggatct attgaaaaaa cagttttaca ccatttgtaa agcaatcgat
    14281 tttaacgatg aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt
    14341 agcctagaag cgaaaacaaa tactaaagag caatatttta gagaaggatt gaaagaatgc
    14401 gcagaaacaa ttgctgcttt tcttaagttt catgacaaag tagaaattga gcctgaaaaa
    14461 attattttta ctttctctag atctttgcca actgatttaa gttatttagc tgaagcttta
    14521 cctaaacttg caccatacgt atccaaacga actattcaga gccaaattcc atttgtaaca
    14581 gatgttgatt atgaaaatga aatgatggaa ttggaaagtg gaagtgctta tccagatagt
    14641 gaatacaatt ttggcggagg tggcaatagt gacgatagaa acgaagtatt ggaccaaacg
    14701 tcgagaactg gaggatcaag caaggctcaa acaagaaaat cagacactta aaaaattaac
    14761 tagtgtattt cctgaagcac tgaaagagat acaagcaaaa ctattatcac aagctgactt
    14821 acacaatatc acttatccag aaatgatgga gttttacagt acaagtaatc agaaaaaata
    14881 tcgtgaatat gtggaaaaaa attataagtc attaaaaatg tatgatgcaa aatacaaaga
    14941 gtttatcgat gaattttttc caccatttga ctatgcaaaa gtcaatcgct tattacaaat
    15001 acgatcagat gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg tgaatcaata
    15061 tttttctgat cgcttagagg aaattcttca aagaacatat tcttctaatg ctaatgtttt
    15121 tgttcaactt ttaaacgttg atataccaaa ttatttacca gaaaatgaaa ttaaacatta
    15181 tttgaactat ccatggtcag gaaagacatt ttcaagaaga ctttggggaa atatttcatc
    15241 gcttgaacag aaactctcta atgctattgt aaaaagtgtt gctagtggag aaggcgttat
    15301 acacgcatta aacaccatga gattagactc agaaatttgt gacatgttta agttagaaga
    15361 atcaaagtat aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca
    15421 agatggtatt gaaaaatcat atttagaaac aggtattgag gaatacaacg tattgactgc
    15481 aaaagatgag agagtttgcc ggatttgtgg aggaaaggca agtaagaatc cctataaact
    15541 gaaagatgct gtcataggtg aaaatcgagc acctttccat agtcgttgta gatgtacgga
    15601 tgttcctaat ttaccaaaat taggaaagga tattgatgaa gaatatgacc gtttatttgg
    15661 cgatttatta gatgagtttg cacatgattc ttttggaatt aatttgaaac ggaggaagta
    15721 gaatgaaaga tttttttgaa gcagtactaa caattaatgt aaatgctgat attgcagaag
    15781 cctacaaaac agctattgag tctgagaacc atcctaatgg cttgagagac cattggaatg
    15841 gcaattatgc ctacgtggtt attggcgatc aaactgttaa ttatcaagat aatactccag
    15901 ttgataagaa taccgttaat ttaacgattc aattattatc tcattcatta ccaaatttaa
    15961 aagaaacagt tgattggtat gaaaagatgg gatgtattgt tgttagaact gactacaaag
    16021 aaggaaagtc tagtaattag gcttttttat tttgtccgaa atgacgttaa actagcgcaa
    16081 tactgggctt ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg
    16141 aaaacgaatc gtggggcgaa aggagaatga gcatgaaaca caaatcatta atgccaatga
    16201 atttgcaata ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt
    16261 ttgtggaatc caatgaagaa gctcaaaaat ttattcagtc acaatctcaa agtgttgccg
    16321 acaaacaatt ggaagcttgg aaacaaaata acttagataa aattaaacag gataccatca
    16381 aggaatatga ggaatctaag aaaaacaagt cacctgagca aattcaactg gaaaaactac
    16441 aagctgaatt tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa gcatttgttg
    16501 cagaacaaat tgctggatta gaactagatg gagagttaaa agagtctatt tctcaattta
    16561 tgctaaataa tcttgttagt tcggatacag atttcactaa gaatgctgtt gaaggtttta
    16621 caagtgtttt gaatgcaatt aatgagaaac atgcagatgc attaaaagaa ctacaaatga
    16681 agtctgcatt tggtggaact caacaatcga ataaccaggt tcagcagaac aatgaaacat
    16741 taacaaatcc agaagaacaa ttaggacaaa ttcttcaaca atttaactag gagagtgaaa
    16801 aattatgaaa aaaacatctt taaataattt agagtatttg gatatttcac cagcgattaa
    16861 tgctatgcaa gtaccaaata cacctttttt aagctattta cttggtgctg gaaaaacaga
    16921 gccagcaaac tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg atgattcttc
    16981 tgaaaaactt gagggcggag aatatccaga tgctgaatca ggtcgaactt ggtttaacaa
    17041 ctatactgaa atttttagaa aatcaacctc tgtatctggt acattagatg ctattaatgt
    17101 gaatggagtc ggaaatgaat taactaatca agtagcacta cgtggtatgg aaatgaaaat
    17161 tgatttgaac cgaaaattga ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg
    17221 tcgaatgaat gggattttga acttgatcaa ttcagcaaat aaggcagaaa cagccactgc
    17281 gggtgcagta acaagaaaag atatcgatac tttatttaaa ttgatgtatg aaaaaggtta
    17341 tatgggagaa aaactatgct tgatttctcc agatatgcag gagttaatga ctgatgagtt
    17401 agatggaaaa tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg gattgcaaat
    17461 tggaaatatc gtgtctaatt acggtacagg tattgctcta ctagaaccat cattgccaaa
    17521 aggaacaatt gccgcaatcg atactaatta tgtgaaacta cgtccattac gtgaatggag
    17581 agcagaagaa cttgcaaaaa caacggattc tagacgtatc ggtcttgtag gtgaatactc
    17641 tcttgaatac aatgcttcaa actctggggc aattttaaat ttaaagtctg aataaaaggg
    17701 agttagtact cccttttttg ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa
    17761 aagatgaagt cgtgaaatat agagtaggta aaactaaaaa ttttgttggg tttgttcatc
    17821 ctaaaactcg tagatttatc acagcagatt caaataacga atttatcatt tctatagatg
    17881 ataaagaagc aattgcaatt ttggaagatg caattgatgt taatgaaatt taggaagtga
    17941 tctgatggat gaatcgctaa aaacggaaat cattgagtct acaaaagaag attttccaga
    18001 tttgagtgaa gaacgcataa ctaatttatt agaaataatt ttgctagaaa ttgaatcata
    18061 caacacttgt aaaaatgata tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt
    18121 gtataaaata ataaaaaatg aatcagaaaa aacagtatct tcagttagac gtggcgatac
    18181 aacgattagt tatgcttcaa caacaaatga tgttagtgag ttacttctag gctatggtga
    18241 tttgatacga agagttattg gttgtggagg attggagttt ttttaatgaa tgaagcagat
    18301 attttagaaa ctacttatga agatagttgt attattgaaa gactgacgga cattgaagat
    18361 tctaatacaa atattactat tcaagattac aaaaaagtat atgataatcc tatttcttgc
    18421 gctctttcac aaggtcaaat cgatggacta gcagtcatag aagatggaga gatggtaaat
    18481 gtttcaactg acacatataa attatttgtt catcctaaga ttaaactaaa aaaaggagat
    18541 cgaataacaa taactcaaaa ggccagtggc ttaatttttt ctctatttgc tactaagcct
    18601 ttttactatc ctagtcattg tgaagtgaat ttgataggaa gtgagaaaaa tgggtaatct
    18661 caaatatgaa tcaaatgcag aaaagatcat tgagaatttt aaaaatatga ctgtaattgc
    18721 tcaaaaagaa ggaatatcct tcgttaacga ttcaatgaat aaagtcgtta gtttagctaa
    18781 accattaact cctgtaaaat caggtaattt aaggcgagga tatcgtgtag taaaagctag
    18841 aaaactatca agcggtcgaa ttgttggagc agttataaac aatgaacatt attttaaata
    18901 tgtagaagaa ggtcatagga ctaaaaatgg tggctttgtc aaagggaaat tcatgttaac
    18961 tcgtgcaacg aatcttgcaa atatgtctta tattcctcga agatttaaac aaatggcaat
    19021 aaaaatcgtt aagaaaggaa agtaacatga aagatgaaat cattgctgca atcagtagca
    19081 aattaaaaga aatctatcct gatgggacaa tatatcttga ttcagttatg cagtcaacta
    19141 aagattttta tttcgtttta tcagtaatgg aatctggaac tgaaaatgta ggaattgata
    19201 ttcaaaatgt ttctttctta attgatattg cattgattga taataaacct aatagaaatt
    19261 taattaatga attagtctca cgttgtggga ctttttttaa tacgattaca atcgatgagc
    19321 aaatattatt tccaaaagcc tatttacctg atgaagcaga tggtgttcaa catattcgtt
    19381 ttacattaga atttccacaa tatattgaat ggagtgaaag ataaatggga gaaaaaagaa
    19441 gtaaaaccgg aattatttct gtcgaaaaac ctacttggtt tcctttggaa gatgagacag
    19501 ggaattttcc ggtatatggt actgcgttgc caatgggaac agcagtaagt atcaaaccaa
    19561 cagctaatta tgaaacaaca caagattatg gggattcagt ggttcaagat caatttacag
    19621 cttttggtgg tgcagaagtt gaactggaag caaatggata tactcataaa gttttatcta
    19681 caattactgg agggaaaatt gttaaaggtg gtgccttgcg ttctggagaa gatattgcgc
    19741 aagatggtgc gtttgcatat agacgtaaaa aatcgaatgg gaaataccgt tatacggtct
    19801 tttataaagg acagtttgct ctagattcag atgaaacatc aacgattgaa ggaagtaaag
    19861 taagttttac tcatccaact tggaagggtt catttgtaga tgtaccagga cttggatata
    19921 tgtattcagt tgatgaagat gatgaaggcg tggataaagc aatgatcgga aattggttta
    19981 caaaagtagc aattccaatt gaagaagcag aactttcagg aggtacagaa taatgtcaaa
    20041 atatcaaaca acgattaaat taacgaaaaa agatgaagaa ggtaaatatg agcaagtaca
    20101 atttaaatct gctgaatttc taccaggccc agtcgtagaa gaagcagcag aagtaatgga
    20161 gggaatgcaa aatgctgtag atggaaaatc agtatcagaa gcattaagtc gagcttattc
    20221 atttattgca gatattttgt ttgaaggaca gtttactggt gaagattatc gtcaagggat
    20281 cgatgctcga gaaattgctt cgttgacagg taagttatta aaatctgtta ctgcaggctt
    20341 tgatgaaact tatacagaaa cgaaaaaaaa gtaagcgaag ctctcaaata tccttttttt
    20401 aaacattcga ttacttatcg agaattagat ttaaagacgc aattactcga agcaggttgg
    20461 acgttaccag aaattgaacg tactgacttt gacgagttaa tgcgtctttt tgcttttcga
    20521 gatgcagtaa aagagcatga ggatgttgag tactacgata acttcaccca attttaggag
    20581 gtgatacttt gaataacgat gacctaattc tgaagatgat actagatgaa tctggtttta
    20641 ctgccggtat gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa
    20701 aaagtagaaa tagtggcagt tcattaggta gtatttggaa aatatttgct ggaagttttc
    20761 tagctagcgg agtaactaga atcgtaggag ctggctttga tctaatcaag ggttccataa
    20821 gtggagcggt tgatcgagta gatacgatga ataacgctct acgaaatttc caaaatatgg
    20881 gatttagtaa ttcagaaatt atgaagaata tcggaaagaa tgggctttta tctcaaggta
    20941 ttcaaggact tcctactgct ttgaatgatg cgataagtca tgttcaactt cttgcttctt
    21001 ctacaggtga tttaactcgt tcaactcaaa tatttaaagc tttaaatgat ggaattcttg
    21061 gtttcggtgg ttcaactgat caagttaatg aagctgttat tcaattatct caaagtttct
    21121 caaacggaaa agtagacgca caaacttgga actcaatgat caacgctcaa cttggaccta
    21181 ctctttctgc tattgctaaa aagatgggaa ttacaatggg agatctgaaa gaaggtttat
    21241 ctcaaggtaa gatttctgtt gaagaattcc aaaatcaatt aatagaaatg gataccaaag
    21301 gtggcggagg acttaaatca ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa
    21361 cctctataca aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta atagaaggat
    21421 taaataaagc attagtagat tcagatttag gtggatttaa gggaattatt gataaagtgg
    21481 gcagttcaat ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat
    21541 ttttaagtaa tttatttaac gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc
    21601 cctttcttgt tcctgcagct accgcttttg gagcgttcat gtttcaactt aaaggtatac
    21661 cagcaataat aaaaagcttc aataatttta agaatgccat aattggcgtt ggaaattcat
    21721 taaagattat gggggcaata gctgctgcta attcatttgt tttaattatt ggtgcagtcg
    21781 ttggtgcaat agctgttttc ggttacttta tggcaaccaa tgaagagctt agaaacaagg
    21841 ttatatctat ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg
    21901 actggggaat tgatacttgg aattctgcta aagaaatggc atcaaatgca gttgagggtg
    21961 tcaaagatgc ttggtcagga acaaaggaat ggttttctaa cacctggaaa ggtattaagg
    22021 atggggccac aggtttattt gataaaacag tagagacttc aagaaatgca gtcgatagtg
    22081 taaaaaatgc atggtctaat atgaagaaat ggttttctga tacttggcaa agtataaaag
    22141 attcagccag agaaaaatgg aatggaatca aaggttctat tatgtcagtt gctggtccat
    22201 tgattacagg tataaagaat gcatttttac atgttacttt ttatctagag accttatgga
    22261 ataatttagt tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc
    22321 cggttttgtt tattacctcc atgatttctg gtggatggga agaaacaaaa aataatatga
    22381 ttgctg
//
LOCUS       DADVXO010000037        22022 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.038, whole genome shotgun sequence.
ACCESSION   DADVXO010000037 DADVXO010000000
VERSION     DADVXO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.038"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..364
                     /locus_tag="KB950_001183"
     CDS             <1..364
                     /locus_tag="KB950_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6857413.1"
                     /translation="RNVLEKQLKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLT
                     NAIKYLKTGSRIEFPDGSTPIAYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYEND
                     LLKEFTALVTELNKHYSK"
     gene            425..1570
                     /locus_tag="KB950_001184"
     CDS             425..1570
                     /locus_tag="KB950_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6857414.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(1651..1956)
                     /locus_tag="KB950_001185"
     CDS             complement(1651..1956)
                     /locus_tag="KB950_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBB6857415.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1981..2412)
                     /gene="ruvX"
                     /locus_tag="KB950_001186"
     CDS             complement(1981..2412)
                     /gene="ruvX"
                     /locus_tag="KB950_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBB6857416.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2409..2675)
                     /locus_tag="KB950_001187"
     CDS             complement(2409..2675)
                     /locus_tag="KB950_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBB6857417.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2930..3655)
                     /locus_tag="KB950_001188"
     CDS             complement(2930..3655)
                     /locus_tag="KB950_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBB6857418.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(4016..4086)
                     /locus_tag="KB950_001189"
     tRNA            complement(4016..4086)
                     /locus_tag="KB950_001189"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4052..4054),aa:Gly,seq:ccc)
     gene            4203..4955
                     /locus_tag="KB950_001190"
     CDS             4203..4955
                     /locus_tag="KB950_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBB6857419.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4971..5450
                     /locus_tag="KB950_001191"
     CDS             4971..5450
                     /locus_tag="KB950_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBB6857420.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5701..7281)
                     /locus_tag="KB950_001192"
     CDS             complement(5701..7281)
                     /locus_tag="KB950_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBB6857421.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(7274..8017)
                     /locus_tag="KB950_001193"
     CDS             complement(7274..8017)
                     /locus_tag="KB950_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857422.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(8193..8969)
                     /locus_tag="KB950_001194"
     CDS             complement(8193..8969)
                     /locus_tag="KB950_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6857423.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(9217..10053)
                     /locus_tag="KB950_001195"
     CDS             complement(9217..10053)
                     /locus_tag="KB950_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBB6857424.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(10064..10993)
                     /locus_tag="KB950_001196"
     CDS             complement(10064..10993)
                     /locus_tag="KB950_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBB6857425.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11185..11538
                     /locus_tag="KB950_001197"
     CDS             11185..11538
                     /locus_tag="KB950_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBB6857426.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(11612..12268)
                     /locus_tag="KB950_001198"
     CDS             complement(11612..12268)
                     /locus_tag="KB950_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBB6857427.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            12494..14428
                     /locus_tag="KB950_001199"
     CDS             12494..14428
                     /locus_tag="KB950_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6857428.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(14514..15737)
                     /locus_tag="KB950_001200"
     CDS             complement(14514..15737)
                     /locus_tag="KB950_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6857429.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATMEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(15731..17629)
                     /locus_tag="KB950_001201"
     CDS             complement(15731..17629)
                     /locus_tag="KB950_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBB6857430.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(17623..18741)
                     /locus_tag="KB950_001202"
     CDS             complement(17623..18741)
                     /locus_tag="KB950_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6857431.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(18824..19657)
                     /locus_tag="KB950_001203"
     CDS             complement(18824..19657)
                     /locus_tag="KB950_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6857432.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(19650..20567)
                     /locus_tag="KB950_001204"
     CDS             complement(19650..20567)
                     /locus_tag="KB950_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6857433.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(20794..21918)
                     /locus_tag="KB950_001205"
     CDS             complement(20794..21918)
                     /locus_tag="KB950_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6857434.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         6922 a         4347 c         3668 g         7085 t
ORIGIN      
        1 gcgaaatgtg cttgaaaagc aattgaaaaa aactattgaa aaaaataact ctataattga
       61 ttctaatctt tctaatgcta tcaataacat cgaagaagat aacctgactg tctcacagaa
      121 gatttattta acaaacgcaa taaaatatct taaaacagga tctcgaattg aatttcctga
      181 cgggagtacc ccaattgctt atctcgcagc tcttctaaat acgcttttac gatatgactc
      241 attaattaat gattcagaaa aaaatacaga tgagaaaaaa gagtatcaag aattttatga
      301 aaatgatctt ctaaaagagt tcacagctct tgttactgag ctaaataaac actattcaaa
      361 ataataataa actgcctcct ccaaccgccc ccacggcttg ctacactatg gagggaaaac
      421 tattatggca acattcaagc aatatgaaac taaaaaaggt aaattttggc tctatgaagc
      481 ctatcttgga atgaacaaaa tgactggcaa acctgataag gttcgtagac gaggttacaa
      541 aacaaaaaaa gaagcccagc ttgccttgtc acgcttgcaa gtggactacg ataaaaacgg
      601 tattaaaaaa gcgaacaacg aaacgtttaa agaagtttat gacttgtggc tagaaacata
      661 taaaaccact gtccgagaag ccaccttcat gaaaactgaa atcaagttta gaaagtggat
      721 tcttccaaaa tatggaaatc ttcgtgtgaa tgaagtcacg gtaaaaaaag cccaacaaat
      781 tgtaaatagc tgggctgaaa ctactgacca atacaaggta cttcattcaa cttctaagcg
      841 tatttttagc tacgctatca atcttgggat tattgagact aatccactag acaacgtatt
      901 aatgcctaaa agaaccacca ctaagcataa agaaaaggtc aaagtttaca gtaaagaaca
      961 actgatcacc ttgtttgatt acctaaacag caaacctgaa acttatcgaa acgactttga
     1021 taaaacgtta ctacgctttc tcttttatag cggggttagg atcagtgaag cccttgcatt
     1081 gaattggtca gacattgatt ttcaagaaaa tacggtcacc atcaataaaa cactaagcca
     1141 atccaaaaat ggctataaaa tcagtgatcc taaaactgat tgtagtgctg gggttcttcc
     1201 actagatgaa agaacaattt ctatattaaa aaaatggcag attaatcagc ggaaatatat
     1261 gctgacactt ggtgtaactg atccgactat gattttttgt ggtatctata aagaactaat
     1321 cacacaccac gctatttatg caagattgat tactatttgt gaaaaagcta tggttccatt
     1381 tctcggtgtg catgtagcaa gacacactca tgcttcaatg ctcttggacg ctggagctac
     1441 tatggtagaa gttcaaacta gattacggca tacaaaaatt agtcagacga tcgattgtta
     1501 cggtcacttg gcaaaagaaa cgaaagaaaa gactgttgaa aaattagtac aacacttaaa
     1561 tattgactag atggcataca atatggcata cagtaaaaaa taaaaaaagg agaaacccta
     1621 tcatatcaag gtttctccta gctttatagt ctattcttct tcttccgcca tgaatgtatt
     1681 gaatacttct tcaatcatgt cccattctgc gtctgtttcg atttgttcta actcgccttc
     1741 tgttccatct tcgttttcga tgtatgcata agcttgtaat tctacatctt catcttctga
     1801 aacaccagca ggatataaaa gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt
     1861 tagtaggatt tcatataatg tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg
     1921 tccttcatgg tcgtgggaat ggttatgttc tgtcataaga ttacctcttt tcttttatta
     1981 ttaaaataat tgcttacctg agccgtctaa ataattttgt aagatcatca cagctgctaa
     2041 tttatcgatg actttttttc gtttggctct tgaagtattt gcctgctcta ctagcatacg
     2101 ttctgcctga acagtggtta atcgttcatc ttgataatca actggcaagg agaatagttc
     2161 tttgatcttt tctccgtatg acatggaagc ttctgccctt ggtccgatgg aattgttcat
     2221 atttttaggt aatccaacta caaaccggtc tacttcatat tgtgctacta attccttcaa
     2281 ccgatcaaag ccaaactctt cttctgcttc attgatccga atgatctcta ctccttgagc
     2341 tgtccagcca aaggggtcac tgatagcaac acctactgtc ttagaaccga catccaagcc
     2401 cattactctc ataaattcac tccatgattt gagaggtagt ccttgacaat tacttccatg
     2461 atctcatcgc gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg aggaatgtag
     2521 gacgggtctc cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct
     2581 tccaaagccc gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca
     2641 aaaccaaaac gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg
     2701 taattccagc ttaccttcta caaccttatt ttactcgaag aaagggaaaa ctacaaacaa
     2761 atattccaaa cttccgaata aattaagaaa cactatttgt atgttttttt ccttttttct
     2821 gtagctttat caaaagtact cttttcataa agtatacgct atatttcttt gaatgtatgt
     2881 ttttacacaa ttttttcata atattcataa aaaattcgaa tagctgctac taattttttg
     2941 tttttgttgc ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca ttgtagataa
     3001 agacattgaa ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc actcccctgg
     3061 ctaccagttc attcccctca aaatgaggag tgacacggta gcgtacatga tgatttgttt
     3121 ctttgatata tgccgcaata tcattttcgt gagcaagcat gtatggagca ttcaacgaac
     3181 gtgtccctgt catcaagttt ttaatgttgt tattttgacc agtcagctgg aaaccaatta
     3241 aatggctgcg attatataac cattgcccgt cacttaattt cttttgtttc caacctgtcg
     3301 ggtcaatata caaaggctct ctcttttcag tagggaacaa atcttttcca agcatagcat
     3361 tggcttgtcc tactctgttc aatttatcaa tatttgaata gctttcccat gttcctttgg
     3421 aaaggctaag atcctctttt gtaaattccg gctgattccc attgatttct atttcttgat
     3481 gttttccatc atattctggc acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa
     3541 tagttgtaat ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc
     3601 ctgcaatcac agcagcgacg atccccacga tcgccttggt tgtttttttg ctcatttctt
     3661 tactccttta ctaacagctc cctaaatctt aacatatgtt cccctataaa taaatactag
     3721 aaagaaatag aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa
     3781 gagacggatt cctcgtctct aatcagtcac cactctatct gtaaggtatt tattattatt
     3841 caaaaatttt cttcatactc aagacaatac actgatttca caaccgcttg gtaaacggtg
     3901 cgcaaaagct tgcatctgcg gtaataagtc catccaagtt aaaaacataa gaagttgttt
     3961 ttgcttggct gttcttatta cttgttgcag agcagctttt tcacaaccta ttttaagcgg
     4021 gtgacgggaa tcgaacccgc gacatcagct tgggaagctg ggattttacc actaaactac
     4081 acccgcaata caaaaacagt atacgcctct tttattcctt cctcaagttt ttttattctt
     4141 tacgcttctg gtatgttttg tagtaaaata aaactaatca atcaattatt ggagaagatg
     4201 ctatgacggg aaaagaatta gatctgttgc tggacacttg tttactcgct gggaaaatca
     4261 tgatggaaag caatgcggag atgtatcggg tagaagatac gatgagtcga attgctttag
     4321 cttctggaaa tttccgtttg gtcagctatg tcactcaaac cggtctcttt attggtcttg
     4381 atcgtacctc gaccatccga atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag
     4441 tagtaaaagt aaatgatctt tcacgtaaat atgttgcttc agagctaaca ttagaagaac
     4501 tttactgcga actgcaagca atcgaccgag accgttcttt ctttcctgtc tggttgcaaa
     4561 tcgttagcgc ggcagtgatc agtggaacaa ttatggtgtt atttggcgga aagatgaccg
     4621 atttacctgt gactttagtc attggaggat tgggatacgc agcttatcta tatagtttga
     4681 aattttttag aatcaaattt ttatcggaat ttttatcttc tttattgatt ggcatagccg
     4741 ctatctacag cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt atcggatgtg
     4801 tgatgccttt agttcctgga gttcaaatta cgaacgctat ccgtgatttg ctagctggac
     4861 attatgtctc aggagtttct cgcggaacag aagcgatgat gactgctacg atgatcggtt
     4921 ttgcgattgc ttttattttc cagctttttt attaggaaag gaacacaagt atggggacga
     4981 tatttattca gttttctttt agttttttag cgactgcggc atttgccgtt attacaaatg
     5041 tacctaggcg ttcattgatt tgttgtggtc tttctggtgc ttttggctgg atgatctact
     5101 ggatctgtgt tcaaataggc ggatctgctg cttttggttc attgcttggt tctttaggag
     5161 tagcattcat cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg attttcaata
     5221 ttcccgggat ggttcctcta gtccctggtg gtttagctta tcaagcggtt cgaaatctcg
     5281 ttactgaatc ctatcgagaa gcggctcatt atgcggtcca agcaattatg attgctggag
     5341 caatcgcttt aggtcttgta ttatcagaag tctttaacca taacatccgt aatttcaaag
     5401 taaagcgtga cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa acgaagtgta
     5461 acaaagaaaa ggttgtgaaa gagacgttca gccttgagcg atgacaaaat atctcacaaa
     5521 cattgcgaca agtaaccgca ggagcaataa gaagtaatta tgagtattaa aaaaagtttt
     5581 gtaaaaatag cttctcacat ttttacataa aattgactta atactgagaa actgactttt
     5641 gaacaccctc tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg cccctcttct
     5701 ttattcaaat tttttccaaa acgttcgttg ataataaacg atccatagag cggaaagtat
     5761 tagaactaaa ccgatcacag gaatattcac taaccaaaat ggataaaaca aagccaacat
     5821 tccgtataag acgataagaa taatactgat gatcaatgaa cccatcatag ttgcgactaa
     5881 acccaaactt cctgaacctc tgttaaataa ttggttaata ttggtccagt tcagcagcag
     5941 caaccgataa tctctagcga aataacgtag tgacaataag tagcagccga ataaagaacc
     6001 aaatagcaag ctagggataa aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc
     6061 gatcccacca gttaaaaaac tctgcaatat ccatccgaat ctgaactttt cttttaaata
     6121 ttggttcata gagattggca aagaacggat aaacagaaaa ttttcttgat ccaaagaaat
     6181 aagattgctg ataaaagaag tctgattaac tgttaagaaa gccaatgcga tccctaccag
     6241 gaaaacaaca ccgattaact tattatctaa atcagttaat tttatttcgc ctcctagggc
     6301 aaaagcaaga ataaaaatga ttggagttaa caaggaagaa gaaagaactt gcataatcaa
     6361 attaggttct tttactaatt gagcattgta actaaataaa agctgatgca cattttggtt
     6421 ctttttatgg gttcttttta ttttgaactc actgtttgaa tcagtcagtt gctctgtaag
     6481 ctttggtact atcagctgtt ttacagcaag gaaaaatccc agagccgtga tagctagtat
     6541 tccccaaccc aataaacctt gttgagaaag tggctgactg gcagcatcat agaatggcaa
     6601 taatatcgaa atcggatgcc gatctgttaa ctcattttca atagtccccg tttgatgatt
     6661 catccacatg attccagcaa atgcgatcag catcgagctg ccaagcaaga tactagaaac
     6721 tgtttttcta tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc
     6781 cagacaaaaa agaataatca aaaatagaga aaacaaaagg attgagataa tgatagtaag
     6841 caaaatccct agatcagctc gccatccggt taaaataaac atgactagaa gcggaaaaac
     6901 aaaaggaatg atggtcaaag caactaccaa aatctttgct gaaaaaacct gttcttgtcc
     6961 aaaaggcaga ggcaaaaata cttgcaggtc atttccttca aaaaagatat tataaatgac
     7021 tgatatactt tgagttaaac ctaacatacc aaaaagtgcc acgtaataag taaagaaacc
     7081 tggcaattta acaaagttca tagcaaacat agcaaatccg taaatcagta aaaaaatcag
     7141 ccctgaaaaa agattttgag acaaaagata gatagttaat cgctttccct tttttccttt
     7201 ctctcttgct ttattcgtga gctgtggatt aacatataaa agattaactc gaattaattc
     7261 taataattgt cgcttataca tacggtccat ctcctgtcgc ttgccttcca gccattttca
     7321 aataaatctt ttctagcgtt tcatcaggtg agccattcaa taattcacta actgccccct
     7381 gataaatcaa ttcacctttc ttcaaaatag ccagtttatc gcataactgt tgtgcggtat
     7441 ccaatacatg tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc ttcatcattt
     7501 ctttcaaatc aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa
     7561 tatctggatc tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa
     7621 aacttgcgat tgtttcattt tgatgactat acatatcaaa aagcgctgtc aattctgcta
     7681 aacgcttttc ttttttttgt gtgtctaatt cataggcagc tgaaatcaag tcccaatatt
     7741 ctccagccgt caattgtaaa aaaatatctg gggaatcagg cacataaccg attttttgtt
     7801 tgatactcaa tcgattttcc gtcaatttca ttccatctac taaaatcgtt cccgatgatg
     7861 gttcaataat actaactaaa cttttaatcg tagttgattt tccggcacca ttatgtccta
     7921 aaaatccaaa aatttcccct tgtcttatcg agagagacaa gtcttttaat gcttgttttt
     7981 tcccgtaact tttacttaca ttgatgaatt caatcatttc tgtactcctt ttgtttgtta
     8041 ttacttttta ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag tgcataacaa
     8101 aaaaggatag aaacgactag gctgctactt gggttgttct cttctacagg aaaacactct
     8161 tctaacaggt aagatatgag tctttgtttt ctttaaaatg attcattttc tatcttttct
     8221 tgcataaaat tctttagttt atcaatactt ctctttccca tctctagttg ttctccattt
     8281 gcaaatgtta ctatttcgtt tactctgcta atagatgtaa tgttttcagt attaatgata
     8341 taagctttta aatctttaca taaatactca tattcgagtg attttttata ttttttaatt
     8401 gtgccgttta cgagaatctc ttcatctttc aatactaggc ctattttatt tttataacct
     8461 ggcatgctca ttagataaag gatttcatcg taatggatat agtgatcgct attaccataa
     8521 cggacagttg tccacctttt tttcgtagca tgttgaacag gatagttttc tttttctttc
     8581 aataagtaac tttggatgct ttttttattt ttttcataag tgaattcttt tatcaaataa
     8641 ccactaggcg taatctttcg attaataaca gttattgctt tattcggatg agacgttaga
     8701 taaacaattt tacacttttc attcttttct ctaagcattt cgcctagttc gataccgcta
     8761 taataggcgt tcaaatcgat gtctaggaaa aaaatatctg aggaatagat ttcattattt
     8821 ttaatgaatt caacaaattg tataacgttc aatccctgaa cgatctcaaa tttccaagga
     8881 cttaataaaa ttctggattc ttccaaaatt tctactagca ttttttcaaa aaccaaacta
     8941 tcatccaaaa taaatatacg cgttaccata aaaagtacct ccactcgata ttttactatc
     9001 aaaatcattt catacaactt ttcaacatac atgttaggaa taaattgtaa ttctcttcac
     9061 aatcttttcc aacacctttg aaaccgatat caaatacttt caggatgctg cttctaaaat
     9121 aaatatcaaa caaatgaata catcatgcaa aaaaagccag acaaccgacg tccttgcgtg
     9181 tcagctgtct gagctatgtt tatttatttt gttcttttaa aaagaacgat acacatactg
     9241 atttttcgga gcagagactt tctcagcgtt tgtaacaact acagatggga tttcacgctt
     9301 gaatggttga taatagtctg attggatggt tccttctatt ttataccatt catcattttt
     9361 aaccgttact ccttccggca tatgcgtcaa caaaccaaag actcctgaat ctgccacaca
     9421 atggataatg ccgaatcgga acaagaagat atctgtattg ctatcgttcg gtgtttggta
     9481 aacaaatcct tcatagctga tttttttgcc aatgaattcg ctagggtaat tataaattag
     9541 ctccataact tctaaatagt tttcatctgt gatcttgata gtttgcttat ccccatactt
     9601 ttctttcaac tcattcattt ggtctaagta gtctgatttg ttgaaataaa tactggtatc
     9661 cggctttaag tattgtgtct gcatctccgg atcccctact gattctttac ttactgggaa
     9721 attaaatcct ttggcttcta cgatcgtagt gtcgagacta actgttggaa acaacgtccc
     9781 cacgattact ggcaaagcta gcagtacata agcgattcca cgttgataag gttttgacaa
     9841 cccatggtca tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa
     9901 atacagctga actaaagcca aaagagcaga taaaatcatt gaaagaatag ctaagtagcg
     9961 ataatggaca ttgatatatt gatcgagttt gccagaaact tgcaagtaca tcatcaattc
    10021 gaaataccca gataaaatca aaaaccgcag catattttcc ctcctaaata aataatgtga
    10081 atccaataac gctaagggca atcagtccga tcagcgataa aataaagcgt cctgtaaaat
    10141 atcgtttcat catcagtaga tttttgatat cgaccattgg gccaaagacc aaaaaggcag
    10201 ctacaggtgc tgtgccgaac agacttaaca aagaagatcc tataaacgca tctgcttctg
    10261 agcaaagaga catcgtaaag gccaataaca gcatgatgat aatggctaaa atctttgttg
    10321 agccgagcgt tagcataatt ctagttggta aataagtctg catcgcagct gcgattaatc
    10381 cgccaatgat caaataacga cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat
    10441 gcccagtttg atggatcagc tgattttttg gttcattctt ctcactttgt tcttcttcat
    10501 ggacatgatg atggtgattt tctgctactt tagacgtgat tttttctttc aatacagcgt
    10561 ctttttgaaa ataggcaagc cagacaccaa tcacaagggc aacaaaaaag ctgcctatca
    10621 cccgccagct tgccatctgc caagaattgc caaatgcgat aaaagtggaa aaaatcacga
    10681 ttggattgat gatcggagca gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt
    10741 ttttaacaaa ttgatgaacg atcggtacga tgccgcattc acatgaagga aagaaaaatc
    10801 ctaagatcga accagtaaaa atagagaaga tacgattttc aggaagccat ctcttcacac
    10861 gttctggggt caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag
    10921 gtaatgcttc aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa
    10981 taaaagaaaa catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg
    11041 cttttattca aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca
    11101 aagcacttca attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact
    11161 tctatcataa aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac
    11221 atactgcaac agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt
    11281 cacgctgttc gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat
    11341 ttggatgcat ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt
    11401 gtcctgattt ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa
    11461 aaaatccgtt atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa
    11521 atcttccgaa caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt
    11581 ttgccttcat ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat
    11641 ttgcgtcaga atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt
    11701 ggatcaatac atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga
    11761 ttcctgcttc cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa
    11821 ttgctacttc gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg
    11881 ggataccgtc tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac
    11941 gtatgctatt actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac
    12001 cgatcagcgc aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa
    12061 gatcttctac atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc
    12121 gacggatcgt tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt
    12181 tctttccagc attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg
    12241 tcgcctttgg aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc
    12301 tttgatttct ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt
    12361 gtgaaatttt attctttatt catttattta tcatttctgc aaatctcgca gtataatgga
    12421 atttatggta gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag
    12481 aaaagaggat tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga
    12541 agtcttattt gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg
    12601 cagaaatggc gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga
    12661 tgaaggaaag atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg
    12721 acttgattcc aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca
    12781 aatggaagag cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc
    12841 tgcttatagt tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa
    12901 cggctatggt tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc
    12961 attttatgat cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc
    13021 tcgcatgctt ttgcaacacc ctgacatttt gattctagat gaaccaacga accatttaga
    13081 tatcgagaca ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat
    13141 tgtttcccat gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag
    13201 aagaaaaatg cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca
    13261 gttagcaagt gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga
    13321 cttcgttgct cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa
    13381 gacattagaa aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt
    13441 tctattcagt atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat
    13501 cggatatgat aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc
    13561 gattgctcta gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca
    13621 ccagatccct tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta
    13681 cgatcaaggt caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga
    13741 gcatcctacc actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg
    13801 cgaagatgta gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt
    13861 agccaagtta gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt
    13921 agatattgac aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct
    13981 ttttgtttcc catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc
    14041 tgaaaatggc agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga
    14101 agaagaagaa atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac
    14161 gaacaaatct gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg
    14221 aaaaattact caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga
    14281 acaagaaatg gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca
    14341 agatttagaa gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag
    14401 cttggaatta gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg
    14461 ttttggtttt tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa
    14521 taagaagacc aattactagt cattctcact aaagcttcta aataatagta atcaccccaa
    14581 atacaaggtg tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag
    14641 ccatcttctt cttccattgt agcatatctt tctttcaatt gtcgcataat tttccaaaca
    14701 gcagactggt ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat
    14761 aatccacaaa ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca
    14821 aagatcaaat cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct
    14881 aatattttgc ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa
    14941 ccgtaaatcc cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc
    15001 gtacttccat atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa
    15061 ccatcttctc ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta
    15121 tatttttgat ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa
    15181 tccatgatca ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact
    15241 cctgccttct cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc
    15301 atttttcgat ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca
    15361 agatcgtgat gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct
    15421 ttttcagctg tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt
    15481 cctgtccaaa atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata
    15541 ggacactcgc ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg
    15601 tgtctgcgac aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat
    15661 tcatttatcc atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc
    15721 ccaccagcct ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc
    15781 cggttgtccc atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc
    15841 gatcttgatg agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct
    15901 ctaatccagg aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa
    15961 tagctgtgga ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag
    16021 cttcactcac cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag
    16081 aacattcaat agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt
    16141 gtacgcgaat ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag
    16201 taggttccca tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc
    16261 gaacgatgaa ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg
    16321 tgtccgctcg ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg
    16381 ctcgcggtat cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg
    16441 tcaatgtatg gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat
    16501 catgttgtat gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag
    16561 gcatttcttt agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct
    16621 tattcagcca taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt
    16681 aggtataacc aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc
    16741 ttatattccg attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc
    16801 acaacatagc tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag
    16861 gcacatttgc accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc
    16921 ggttcttata tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat
    16981 aataatgaaa tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc
    17041 agccattttg aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt
    17101 taactggata accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat
    17161 taccatctgc acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt
    17221 ctcctgtatc taattttttc cataataatt gttcatgatg atataacgct aataacattg
    17281 cgggcatttc taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg
    17341 aaggatctgt tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt
    17401 ggtcaattgc gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt
    17461 tatttccata tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa
    17521 acggctggag tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta
    17581 atagtggtac tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc
    17641 cgactgttct aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac
    17701 attataagtc agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc
    17761 catattccaa tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg
    17821 atacattttt gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc
    17881 gcatactcca gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat
    17941 ataaccagtt ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag
    18001 atagtacgga ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt
    18061 tcctctgctc caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt
    18121 ccctgattct gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata
    18181 cttagctgtt gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc
    18241 cctcgaagcc caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg
    18301 tttatggtca ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac
    18361 cgtagctgca tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc
    18421 aatagccgat ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag
    18481 ggcagattct aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt
    18541 cttgtgaaag agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc
    18601 tctgtccttt attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc
    18661 cattctgtct aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca
    18721 ttccttcgtc tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag
    18781 ttgctcctag aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc
    18841 caatgtcatt ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca
    18901 ctgaagttgc taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa
    18961 ccgtcatatt tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta
    19021 atggaaatag atattgattc caacttccca taaagataag taaaccacta gtaataatcg
    19081 taggtgataa aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga
    19141 ttctggcagc ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca
    19201 ctgaagttgt actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta
    19261 aacgactcat aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata
    19321 gcataactgt attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac
    19381 ccgccattgt agaactggct gtagctaaaa ctgtactgat tacagcaata acaatcgaat
    19441 tccaaataat caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca
    19501 tatccccaaa cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag
    19561 ctacaatcat atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt
    19621 acgtaaaaat tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat
    19681 tgaatagccg ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt
    19741 ccaaaatcaa ccatttcaaa acttgtttta tataaattga ctcctacgac caaagctgaa
    19801 ttttctggtc cattggaagt cccaaagaat atatttggga tattgaaatt acccaagcca
    19861 ttgatagtgg ctaaaactgt ggccattaaa atgattggct ttagcatggg gatcgttaat
    19921 aaaaagaatt gccgaaattt cgatgctcca tctatttcag cactttcgta tatctcttta
    19981 gaaatccctt gaagaccagc taaaaataaa atagtgttat atcctacact aagccaaatc
    20041 atcgtgatga tcattgatat tcgtatccaa aaaggatttg ttaaccactg tacaccgtta
    20101 attcctagcg cattcaaaaa tccattgaat aacccagtcg gattgaaaaa agtcatgaat
    20161 acggaagcta cagccaccgt tgatgtaatg gttggaatat aatagacagt tctaaaaaat
    20221 ccttttcctt ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc taatagcatc
    20281 gttattggaa ttgatcctgc catgataatc aaaacattcg tgtaagattt aataaacaat
    20341 ggatctcgga agatagctag ataattgtca aatccaacaa attcaaatga gtagccaatt
    20401 ttcataaaac taaaagcaaa cgagataatg atgggaatca aaatcgtcac aataaagaaa
    20461 ataacaaaag gtgtgaccaa tccataagca ctttttttat gagacgtatc tgttgctttt
    20521 ttcttgctgt tctttattgt cttgcttggt aacgaagatt gcatcatcgt taagtcctcc
    20581 taatattttt taatcaccag ttaattttac gccgtactta tgagcaaaat tttctgcttc
    20641 ttttagatat gaattttcaa aatcacctgt ttgccaatag ttatatgcag cggtagtcaa
    20701 ataagacgta gcatctgaat aagtggctgg taaaacagta tttgatgtat tttcattgat
    20761 tttattcaat agttcatagt atttttgtcc tccttagtag tcaaactttt tctcagctgc
    20821 ttctgtttga taggccaatg agttagatgt agccacgccc agctccagtg cgttggctaa
    20881 gcattcttca tctgtcagca taaatgtaag aaattgttgt gccacttgtg gattttttgc
    20941 atctttaggg acataaaaac ttgatcctcc tgatactgca ccttttcctg gattttcttt
    21001 tgaaaaagga atcagtggag ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga
    21061 catattcgta gcataccagc cgccagcaac aaacattgca gattcttgaa atgctttctc
    21121 agcatcttgt gcaccgtaga aatgtacaat atctgagtcg atcatttctt tgattaactt
    21181 tgcagcatgt ttcgcttctt tagtattcag acggatattt ccttcttcat caagcaaaga
    21241 ttgattctgc tgggctagca tgttgataaa caattcttgg tccccagatt ctggcaaggc
    21301 aattccaaaa ctaccagttt tttcttttaa tgcttttgcc acctggatat attcttccca
    21361 agaactgatt tcttcaaaat ctacacctat ttcagcaaat ttttttggtt gataataaca
    21421 aagtgctggg gtaaaatcac tagcccaagg aataatcgtt tcattactcg tttgttgacg
    21481 caaaatattc aatttttgat cataaaactg atcgccatat ttgtcataaa atccatattc
    21541 atcagcacta taaaaagcat cttgaaattt ttccagcaca cctgctgctg aagtgtctgc
    21601 catggagata atatccggca tatcttcgtg tgttaccaat tttggggtga tcatttgtac
    21661 tgttgctgtt cccgaagcta cattcacaat ttttaagtca atatttgctc cagttttgcg
    21721 gttatagatt tttgcagcat tttcaacata gtttgtcagt ttgccgtttg cgacatatac
    21781 ttctaatgta gtggcttttt ttatcattgg atctgcgatg tttgctgtta cttcttcgtt
    21841 agtttttcca catgcactta agctgaataa tagggtaatg tttagcaaag ctatcaccaa
    21901 tttgtatctt tttttcatgt ctatcccttc tttcttttca agttcttata taaaacgctt
    21961 tcacttatgt atattaacgg aattagtaac acaaatattt atataaaaaa atattttact
    22021 tg
//
LOCUS       DADVXO010000038        21951 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.039, whole genome shotgun sequence.
ACCESSION   DADVXO010000038 DADVXO010000000
VERSION     DADVXO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.039"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            21..542
                     /locus_tag="KB950_001206"
     CDS             21..542
                     /locus_tag="KB950_001206"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6857435.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            543..860
                     /locus_tag="KB950_001207"
     CDS             543..860
                     /locus_tag="KB950_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6857436.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(1017..1181)
                     /locus_tag="KB950_001208"
     CDS             complement(1017..1181)
                     /locus_tag="KB950_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBB6857437.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1284..1805
                     /locus_tag="KB950_001209"
     CDS             1284..1805
                     /locus_tag="KB950_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB6857438.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1792..2265
                     /locus_tag="KB950_001210"
     CDS             1792..2265
                     /locus_tag="KB950_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBB6857439.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(2272..2430)
                     /locus_tag="KB950_001211"
     CDS             complement(2272..2430)
                     /locus_tag="KB950_001211"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBB6857440.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2523..3284
                     /locus_tag="KB950_001212"
     CDS             2523..3284
                     /locus_tag="KB950_001212"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6857441.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENYDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3504..4823
                     /locus_tag="KB950_001213"
     CDS             3504..4823
                     /locus_tag="KB950_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6857442.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4839..6059
                     /locus_tag="KB950_001214"
     CDS             4839..6059
                     /locus_tag="KB950_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6857443.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6094..6771
                     /locus_tag="KB950_001215"
     CDS             6094..6771
                     /locus_tag="KB950_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBB6857444.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6768..7964
                     /locus_tag="KB950_001216"
     CDS             6768..7964
                     /locus_tag="KB950_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6857445.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7979..8302
                     /locus_tag="KB950_001217"
     CDS             7979..8302
                     /locus_tag="KB950_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6857446.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8306..8641
                     /locus_tag="KB950_001218"
     CDS             8306..8641
                     /locus_tag="KB950_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6857447.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8638..9033
                     /locus_tag="KB950_001219"
     CDS             8638..9033
                     /locus_tag="KB950_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857448.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9030..9401
                     /locus_tag="KB950_001220"
     CDS             9030..9401
                     /locus_tag="KB950_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857449.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9417..9977
                     /locus_tag="KB950_001221"
     CDS             9417..9977
                     /locus_tag="KB950_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6857450.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10104..10415
                     /locus_tag="KB950_001222"
     CDS             10104..10415
                     /locus_tag="KB950_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857451.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10439..10630
                     /locus_tag="KB950_001223"
     CDS             10439..10630
                     /locus_tag="KB950_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857452.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10630..14475
                     /locus_tag="KB950_001224"
     CDS             10630..14475
                     /locus_tag="KB950_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBB6857453.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14476..15348
                     /locus_tag="KB950_001225"
     CDS             14476..15348
                     /locus_tag="KB950_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBB6857454.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15345..16394
                     /locus_tag="KB950_001226"
     CDS             15345..16394
                     /locus_tag="KB950_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857455.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16398..17222
                     /locus_tag="KB950_001227"
     CDS             16398..17222
                     /locus_tag="KB950_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857456.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17240..19372
                     /locus_tag="KB950_001228"
     CDS             17240..19372
                     /locus_tag="KB950_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
                     /protein_id="HBB6857457.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19389..19538
                     /locus_tag="KB950_001229"
     CDS             19389..19538
                     /locus_tag="KB950_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857458.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19535..19981
                     /locus_tag="KB950_001230"
     CDS             19535..19981
                     /locus_tag="KB950_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857459.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19983..20120
                     /locus_tag="KB950_001231"
     CDS             19983..20120
                     /locus_tag="KB950_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBB6857460.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20157..20450
                     /locus_tag="KB950_001232"
     CDS             20157..20450
                     /locus_tag="KB950_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857461.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20473..20670
                     /locus_tag="KB950_001233"
     CDS             20473..20670
                     /locus_tag="KB950_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBB6857462.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20681..21700
                     /locus_tag="KB950_001234"
     CDS             20681..21700
                     /locus_tag="KB950_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBB6857463.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
BASE COUNT         7636 a         3758 c         4870 g         5687 t
ORIGIN      
        1 tggattaagg aggaagagta atgcatatag gagataagat acataaattg agacggctta
       61 ataacgaaac aatgaacgac ttagttaaac aaaccggact gtcaaaatca tgggtatctg
      121 atattgagtc tggaaaaaag caacgcgctg actttaataa gatatgtgca atagccaaac
      181 attataaagt tagcttagaa tactttcagg atgattattt ggatagtgat gaacacgagg
      241 atattattcc tttgacagac gaaacaagag ttaaatcagt gaaatcgctt gaacaaagcc
      301 taaagtatct gaataacaaa caagaaccta accttctaga gatcaaacta aaagatactg
      361 actcagtacc agaggtttat tacaaaggtc agaagttaga gggattgata gatgtaagct
      421 atcactacca tacaacaact gatgtggtag ctggttcaac aaacgacatc aatatggagt
      481 acctaggtga agtaactaag tatccagata taaaaacaat cggacacaag agagatgtgt
      541 aaatgaaaga agctagacct agagacgaga tagacaaact atacaagacc aaacgatggc
      601 gagacctaag gcaagtagta atagctaggg acttcggcat gtgccaagag tgcaagcgtc
      661 gagggcggaa cacaaggggc acgatcatcc atcacatagt cgaggcgagg gaagacctgt
      721 cactgttctg gtccgtagat aaccttgaat gtatctgtgt agcttgtcac aacagagagc
      781 atccagagag atcaggcggg aagaagaaac caaaacctaa atcacatatc gttaaaatgt
      841 attcaactcc tgaaagataa gtttgcagcg aaatgaaggt agccccccta ctctaaaaga
      901 ttaaagagta aggcttgaga agaacggtgc tgtccttcct tcgtaaaaag accgcttttc
      961 aagttttttg gagaaaaagg aaaaagccga ccaatttaag ccggctttgg acgaagctat
     1021 ttcttagtcc atttgtttcc tttttgagaa gtaggaggta atcggtcgcc tggatcaatt
     1081 tttacttctc gtccgccttg gacatttcca ccacgaggtc ccacttcttt ataggttcct
     1141 tttggtttat tgtcttcgcc gggtttatag agttctccca taggaatccc tccctaaaaa
     1201 aatttcggca cagcactgcc gataacttaa ttataaggat tgtgataacg atttcaatat
     1261 atcttttgaa agaaggtgat attatgccgc aaccagcgaa gagtgcaaaa ttacaattat
     1321 taaacggaaa cccaaataag aagaataccg aagaactccg caagcgagcg gccgcagaag
     1381 acaaattaaa aatggctact gacaaaatca aaccgccgtt atggctagat tcgctaggaa
     1441 aggatacctt tgagtttatc gctgatgaat tgctgtctgt ggatttaatc agtaatccgg
     1501 acgtccatac aatggctctc tactccaatt ggtattcgca atacgtttct ttagaaaaac
     1561 agcttcgaaa actacaacga gagtacaagt tgaactatgc gcttgcgaaa aaggaggcag
     1621 aggcgagagg tgagccgttt aatgaaccta atgaattaat tggtaatccg ctctctcggc
     1681 agatggatac agcgtcgcgg aatctccgtt cttttggcgc tgatttagga ctatcaccag
     1741 cagccagagc taagttagct attaagatgg ctgatgatgg tggtgatgac gatgacgact
     1801 tctaatattt tggatatgtc ctacacagaa cgtgtggact attggcaaag ctatcttgag
     1861 gagcaagctt cttggggtgg ctttttaaaa tgtccatatc cggaattgtt aactacttgg
     1921 tatgcggaac gattaatcga tggaagcata ccagccagca aagaaaatat tcaagctgct
     1981 aaacggcata tgcgtgattt gcagcgccaa ggaacagatg attttccttg gatctttgac
     2041 gaagaaaaag gtcaccggcc tattagatat atcgaaaaaa aatgtaaacc aactgaaggc
     2101 gactttggtt cgtttgtttt gcaaccttgg cagcatttca taattggatc catgtacgga
     2161 tgggtacatc gtgatacagg agagcgtcgc ttccgcgagg ctcttatttt tgttggacgt
     2221 aaaaacggta agttttgccg tttaaaaatc gggcaaaatc ggtgaaaacc tttatttttg
     2281 gctttctttt agtaataatt cgatagcttt atcaagcaat ttagacatag gaaccatagt
     2341 ttcttccgac atttttttta atccttcgta taaatcttta tcaattgcgt tagaaattct
     2401 aattctgttt tttagtccgt atttgtccat gtgtgacact cctttttatt ttactataac
     2461 atccgattcc accgcttgca actaccatca attgatggta gtatataaaa gaggtgagta
     2521 gattgaaaaa atacattatt tacaaacact taaataaaat aaatggcaaa atttatatcg
     2581 gtgtcacaaa tgatattggc agaaggtgga ggagcggtgg gattgaatat aaaccaccaa
     2641 agaacgaaac ccaacaccac agaagttttt ggaatgcaat acaaaaatac gggtgggaaa
     2701 attatgatca tttgattatt gaagaagact taacgatgaa agaagctttt gagaaagaaa
     2761 agttttatat tgaactttat gactcaacta ataagaaaaa aggttataac atcgcaaaag
     2821 gcggaaatgg cggaataata tataaagttc accccaaagg aatgctaggg aaaaaacaat
     2881 ctaaagaatt ttcttcaaat cacagtaaat gggcaaagaa ccataagaat aattgtatga
     2941 caaacggtga tgttgtatgg ggtgttactc acgaacaccc aaaaggcatg ttgggaaagc
     3001 accacagcaa agaatcaatt atgaaaaaga aagcatatag tggtgaaaat gctgtaacct
     3061 caaaaccaat tgtcgcaatt gaacgggatg gaactaaaag agaatttcac agcgctaagc
     3121 tttgcatggc ttactattcg attagtacat gtgtttttta ccgactttta aaagatggcg
     3181 ccccttatgt gataaatcct aaagcgaact acagaaataa agaaaaaata ttagctatcg
     3241 aaggaattat gttcaaacat aaagaagata ccgaggtaag ttagtagatt gcgaaaggtt
     3301 actaactacc gtaacgcgta ggagttgaat aaatataatg ctcccaagag tgtccgacag
     3361 cagataattt gctgaaaatg tacgctaaac tgggccagaa ctgactgacc gatgaaaatg
     3421 agggtgacct ccagagtgcg agataaaaag ctcgcagata ataacaatcg aaaacatcac
     3481 taatttcagg cctttccaca tacatggtcg cttatgatga tgaacaaggc gccaacgttt
     3541 acgtattggc aaatgctcgt gatcaagcaa gcttgttgtt tgataaggcc gcagaaatgg
     3601 tcaaacaatc gccggcgctc tttaagaaat ttggtaagcc taaacgatca agtattaatt
     3661 atgctcccgc cttttctaaa atggaaccac gcgcctcaga tagccggaaa ttggatgggc
     3721 taaacactca ctttggtatt tttgacgaga tccacgagtt tacgaattac aatctgatca
     3781 acgttatcaa gaaatcaaga ggaaccagaa aacagcctct gatagtttat atcacaactg
     3841 ctggatatgt attagatggt ccgttgatgt cttattttga gcaaggtgtg gactgtttgg
     3901 aacatttgga agatgacatc gatgaacgga ctttctatta tctggcaaaa cttgacagtg
     3961 cggaagaggc tgatgaccca agattatgga tcaaagccaa tccgaatatt tgtctaatga
     4021 attttgttgg catgctagat gactatgtta aggataaaaa agatccaaaa gaatatgctg
     4081 actggattac caagcaattt aacttgtttt ccgatatcga tgagctgtca tttgtcgata
     4141 tgcctaccat taaacgaaac aataaaacca tcgatattga aacgctcaaa ggtaagaagt
     4201 gtgtcggtgg ttttgacttg tccgaaacgg aagactttac cgcagccgtt ttagaatttc
     4261 cgcttgaaac aggcgaggta ttcattttgc aacacacatg gatcccacaa gctagatttg
     4321 atcgagataa caatcaagag cgtatcaaag cgtgggagaa ggtgggagat ctaacgatta
     4381 ttcctggtga ttacgtcaat tatgaatacg tcttaaattg gtttgtagaa aattcgaaaa
     4441 tctatgacat tgtaaaaatc aattatgaca aggccaaggc gctacgatta aataaagaac
     4501 tagaaaatgc aggatttgaa accaacgaga ttcggcaagg gtttctatca ttaggtgggc
     4561 caatgcaaaa cttcaaggaa atgctattgg acggtaaggt gattttcaac aattccaagc
     4621 tttaccgatg gtatctatcc aacgtcaagc tggtgatgga tcgcaactca aactggatgc
     4681 cgtctaagca gtccaagagt agaaaaatag atggttttgc agcaagtttg aacagccacg
     4741 ccgaagtgtt gaatatgttg gttaatcctg tcggaaccgg gaaagtaacc tattactcga
     4801 tttccgattt aatgaatatg taagaaaggt gtggaggaat gagtatttta gatcgtttgc
     4861 gttcttttgg ccgagcgaag ccgaaagcga gcaaacaaga gtattttttg aatgacccgg
     4921 gattgatacc gtatttagtc ggaaaagatg aaatatcaga agggattttt tccgtaatta
     4981 gccgtgtatc gaacgttttt gcgtctctcc ctctcaaaat gatagatgcg gagtttggcc
     5041 aaccggacga ctgtcctgca tacaacttgt tgagcgaagg ccctcgatat tttacaaagt
     5101 ttgatttttt ccgggacgtg gaagttttga gaaactacca agggaatgcg tatgtgcaga
     5161 ttttccgaaa tatcaatgga gaagtagcag atatggcgtt aataaaacct ggtgcttgcc
     5221 atccagtgat tgatatggat agcggggagc tttactacca agtaactgcg actgacaaag
     5281 gcagttataa gcaagttatt tatgtacatt acatggaaat gctccacttt aaacaaccga
     5341 ggtttggtgg cttggaaggt acagacccca caaaagtatt aacgaatacc ctcggatatg
     5401 atcgagaagt ccgaaaaatc tctttaagtc agcttaaagg aggtaatgaa gggctaaaag
     5461 ttaagtttgc tagcaatgtg gatgaagaag ctaaaaaagc tacagttaaa aacattgctg
     5521 atttttatcg acaaaacggt ggactacttg tggaagaaaa cggtgtagaa atcgaacgtt
     5581 tacaacgaga gctggtagac agcaagcttt tagatactga taaagtatct cgctccagaa
     5641 tcgcgatggt ctacaacgtg ccggaacatt tcattgggag taaccagtcg agttactctt
     5701 cacaggaaca gctcaatatg gagtttttga catacaatct agtaccgacc gttaatcaat
     5761 atgaagcgga actaaataag aaaacactat cgagaaccga aaaagcaaaa ggttatcgat
     5821 ataagtttaa tgtcgcaagt ttgctaagag ctgacactca ggcaaggggg caattctatc
     5881 aaatcatgcg acgaggcgga gcatactcag cgaatgatgt ccgaagattt gaggacttgc
     5941 aaccgataaa taaaaccggt atggatgatt atcatatttc cggagaccta tatccaatcg
     6001 atatggatcc aacattaaga aaaacaaact cgtctaaaag cgtagccgaa aacggttagg
     6061 cttttttagt ttgcaccgaa gggaggtgaa aggatgaaaa aagtgacgtt aagcggcgat
     6121 gtcgtggata acgataccgc gtggttatat gactggtttg ggattgattg tatctcacca
     6181 gggaaaattt ctgccgctct tacagaagca gcgggggatg aagtagaact tgatatctca
     6241 tctaacggtg gggatgtcct agcggcaagc gaaatatata ccgctatccg tgcctatccg
     6301 ggaaaggtat ccggaaatgt tgtgagtatt gcagcaagcg ctgcaagtgt aatcgcttgt
     6361 gcttgcgaac cgcttagaat ctcgccaaca gcacacatca tgattcacaa cgcatgggtg
     6421 accactagcg gcaacgccga ggaattaaaa gccaatgcgg aaatgttaag cagtgtggat
     6481 gagtctatcg ttaatgctta cgagatcaag acaggactcg accggaaaaa gcttgctgat
     6541 ttaatggcaa aagatacttg gttaaacgca caaacagcag tggcggaagg ttttgcggat
     6601 gaaattatgt ttgcagaagc accagtaacg gtactcaatg cctctcaacc ggttattcca
     6661 aaaaacgcgg taactaagtt gaaaaatcta atactcaaag cggaaacacc gcaagaagaa
     6721 acgctcttgc agaaaaaact aaaagcctta aatggaggga aaaacgaatg aatttagaac
     6781 aattaaaaaa tgcgtgggtc gaggcgggaa gtaaagtctc tgatttaaac gcgcaactca
     6841 atgcagcatt ggttgacgat gaaaaaacag aagaagatgt agtaagtttg caagcacaag
     6901 taaaagcagc acgggctaaa cgggacggat tgaaagagca agtggcaaat atggaagccg
     6961 aacaagtctt aaacgtcaaa aaagaaccat tagataaaaa agatgaaaac ttgaaaaaca
     7021 agtttatcaa agactttaaa gcgatggtca atggtgatcc tgctattatg gctactttga
     7081 catctaatac ggatgaatct ggtaatgcta tcggattgac tattcctgta gatgtgcaaa
     7141 cgactattca tactttggtt cgtcagtttg actctttgca agaatacgta aacgttgaaa
     7201 aagtgaccac tactagcggt tctcgggttt atgaaaaatg gtctgatatt aacccactga
     7261 ccgctttgga tactgaagac ggtgaaatcc cagcaaatga tgatcctgca ctttacttga
     7321 tcaaatactt gatcaaacgc tacgcaggta tttctacagt aactaacagc ttgctaaaag
     7381 atactgccga aaacattttg gcatggttgt ctaactggat cgcgaaaaaa gtagttgtta
     7441 ctcgcaatac aaaaatcttg gcagctattg atggaatcaa agcagcgcaa aagaaagatg
     7501 ttacagatgt tgatgggatt aaagatatca taaacgtcca acttgatcca gctatcgaag
     7561 ctacatctat gtttattaca aaccaagatg gcttcaatgt tttagataaa gtgaaacgtg
     7621 ctgatggatc ttacttgtta caaaaagacg tagcttctgc aactggatat accttcttag
     7681 gtaaaccaat caagaaaatt gcttctcgtt tcttgccaaa caaaggtacg caagctactc
     7741 ctaaatatcc actgtatatt ggtgatctga aagaagctgt tacattgtat gatcgcgaaa
     7801 acatgagctt gctgacaacg aatattggcg ggggggcttt tgaaacagac accaccaaag
     7861 tacgggttat cgatcgattt gatgtgcagt tagtcgatga tgaagcagtt gttttagcta
     7921 catttacatc tatcgcaaac gaagtgccag taacaaacgg ttaaggagct gatttcttat
     7981 gattcttgat cctaaaatgg atttagacga aatcaaaaac gcactaaaga ttgataccga
     8041 tgatgacgat gtggaagtaa gacgtgcggt gcaagctgca attgcataca ttagaggggc
     8101 tatcgggaat gataagccct ctttttataa gcaagaaaat gacacggttg atctgattaa
     8161 tttagctatt ctgcaattag cagatcatta ttacaacgct cgatcagcaa ccgtgagtgg
     8221 gaacttgcga gagtacgatt taggttttac aagcctaatc ttgcaactca aagcaagata
     8281 tttgcttttt gtggaggagt agcgtatgcc ccttattcaa acaggaaatt taaatcaacg
     8341 catcaagttt gtccgagata cgacggtcaa ggacgaggac gggcaagttg tccggacttc
     8401 tacaaccatt cttacttgct gggcaagtgt gcagacacaa cgcctgaacg atattaagac
     8461 gtccattggt acggctttgg aaggaacact gacgttcatt atccgctatc aacaaaaatc
     8521 agagctaacc aatgatatga aagtgcgttg gaatggaaaa acgtttgaaa tcattacgat
     8581 tacgaaaggc gagtttgcga aggacttcac gacaatcatt gcaaaagagg tttcaaaatg
     8641 agtgtagaag tcgatgcaac cgaagtgtac aaagcgctta gggaagtaaa agcaaacgtt
     8701 caacgagtgg aaagcccagc acttagaaaa gctggggagt acgctcaaga aaagttacaa
     8761 caaaacacac cttactggga tggaacgaag tcaaacggta aacgtggttc gtatatgcaa
     8821 gaacatgcta aggaccatgt ggttacaagc tcggtaaaaa acggattgat agaagtcggc
     8881 tatgataaag atgtttcttg gcggatgcac tttatcgagt tcggaacaat caaacaacgt
     8941 ccaaaaggtt tcgtacaaaa aacacaaaag caaatcgaaa aacaagtaac acaaatcatt
     9001 gctgacgaag taaaaaggag gctaggactt tgaaaacggc agtatcacaa gtctattcaa
     9061 ttctgaatag caatgaaaaa acaaagaaca ttgattttta caccaatagt gttccggaat
     9121 cggctcaaac agtacctagc cttccagttg gcagaattac agagatattc ggcaactatg
     9181 aagatttcgc aagcaacaat cctttgacca ttcaatttaa cgtacaggta gatgtatggg
     9241 tatcgacctt aaaagaggtt gatgcctttt attttgccct tgatgagatt atgaggggga
     9301 atggttggca atgcgcatac acggaacaaa cagatgatga ggatttagaa ggtgcaaagc
     9361 ggattatcaa acgatatgta gcaaatattt cactaaatta aaaggagaga aaatagatgg
     9421 caacagtagg atttgagagc gtcatttttg gcgtaaaaac aggtgcaggc ggcactctaa
     9481 aagaattagt agcagataag tcgaaaggcg gagcgatcga agctaaaatt actggattag
     9541 gcgcaacttc taacacaaca tacgcttcaa acgtaccatt cttcattgca agtaaagggg
     9601 tttcgtcgcc aaaagttacg cttgacgtgg cagacttaat ggataacggc atttacagcg
     9661 aaatcattgg cgctaaaacc gtggatggtg taaatgtaat tggttcagaa actgaagcgc
     9721 cttacgtgtc ggtagtcatg gttacagcga acaaagaagg aaaacgctta ttcatgggat
     9781 tggcaaaagg aaaattcagt catccagata tcgacatgaa aacagctgaa gacaaaggag
     9841 ttgaattgca aaccgattct atcgaagggg aattcatttc tgatgaacgt ggctatgtat
     9901 acttaacagc cgtagaatca gaagaaatga ccttacaaaa attcaaggac ttggtaaata
     9961 acaaagcggg ggagtagtta accctgcatc tacaccaacg acagatacag ggacaccaaa
    10021 agaaccagaa ccaaaaattg atacacaagg ttagccattt tttggctagc cttatttttt
    10081 tgtaaaaaca aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa
    10141 aaacattcaa acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg
    10201 agaaagcgga aaaagcagac ctaactgatt tagaagcaat tgattcaatg attgcaatta
    10261 cagcagatat tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg
    10321 cgagcgagtt agtaccggca ttagaaagtg tttttgaaca aatcaatgaa ctgggaaaca
    10381 atgaaaaaaa gcagacggcg agcaaaaaga gataagtttt tctgaagcta ggaaagcaat
    10441 ggatcaaatc tacaaagatt taatcgaatc aggttggacg atgagagatg tggacgaagc
    10501 cgactatcat tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga
    10561 tggtgctgat ttcatcaaac aatttttatc ggctgaagac ttggtaaaac ttgaggaagg
    10621 aggtaaataa tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta
    10681 gatagcacta attttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag
    10741 tcgaatatgc gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt
    10801 agtcaaaaga cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa
    10861 ttaacaaagc gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct
    10921 aaccttaaca cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg
    10981 aatgacacaa aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaacgaa
    11041 atcaaagaaa atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat
    11101 gaatctggtg cgtttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag
    11161 aaagctatcg aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca
    11221 gcaaatgaaa ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata
    11281 tctagcaggc aattagaagc actcaaaagc tccagcgatc aatcaggaaa aaaaatagaa
    11341 gattttggcg acaagtccac aaggtcagct aggaaactgg atgggctaaa agacaaatta
    11401 ggctcgctaa aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt
    11461 agcagtgtag taagtggcgt gcaaggcttg gttggcgaag cagtaaacgc atcggattca
    11521 ttgatgaagt tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc
    11581 tcgaaaaaag aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg
    11641 aacacaaccg cacaattggc atctaacggg attcctaact atacagaact aaccaaggcg
    11701 gcaggtaact tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg
    11761 atgctaacgc agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca
    11821 gatgcgatac cgggcgcttc agggctgtta caagacgcta tgttgaaaaa cggagcttat
    11881 acaggaaact tccgtgatgc aatggcgcaa ggtcaaatca cttctgatga gtttaaccaa
    11941 gcgattgtac agttaggtat gaatgacgga gcagttaaag cagctacttc cacagataca
    12001 ttgagtggtt cttgggaaca gatgaaatct actgtaataa atgggttaca aagtatcata
    12061 gacaaagtag gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa
    12121 aattctattc ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc
    12181 gttgaaaata gagagccact aacatggatt gtcggaatca taggcggaat tacattagca
    12241 gtaaaagcat tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc
    12301 ccttttgtgg cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat
    12361 acaaaatctg aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta
    12421 gttatgagcg ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag
    12481 tggctttggg aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg
    12541 aagaacagcg ttgtgaatac ggttcagaac ctatggaatg gtgtgaaaaa cttcttcagt
    12601 aatggcgttg gagacacttg gaataaggta gttggctggg taaaaaacat tttcaacaaa
    12661 gcaactgaat tgaagaacaa agtttctgat gcaattggta acctgtggaa cggtatcaaa
    12721 gacacattcc gtagaggcat tgatacggta ttcaattggt tttcagaact accgacgaag
    12781 atgaagaatg ccattattgg cggtaaaaat gccattgttg atgcgttcaa aagtattttc
    12841 aacgcagcac ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg
    12901 gtactagaaa aactgggtgc tgacaaactc gaagaatgga aagtgccaca atacgcaaaa
    12961 ggaacaccaa acggaggtca tccgggcggg cctatgatgg taaatgacgg tagaggtgct
    13021 gaagcggtaa tcacacctaa cggacaagca tttatcccac gagggcgaaa tgtagtgttg
    13081 aatgcaccaa aaggcacaca cgttctaaca gctgaagaaa cagcttatat gactggaaac
    13141 aaagcaccaa gatatagata cgccaaaggt acaggctttt tcggaaatct atggaacaac
    13201 gtcaaaggat ttgctggaga tgttggaaac aagctgaaag atgtagtcgg cgatgtatgg
    13261 gattttgtaa cagacccggg agcgttggct aggaaagtgt taaatggtct tggcgtactg
    13321 gaagggcttg tcaaatatcc tttagatgtt ggtaaaggta ttctaagcaa ggctaccgaa
    13381 gcattgacga acaaaatcac agaactattc agcagtggca gtttagacac ttcaatgggc
    13441 atgcaaggcg tttacaaata cttggcggac gttgcagttg cagtaatgaa gaagtttcca
    13501 ggctttgtgg caactagtgg gtatagacca ggtgacccct attcgcatgg taaacgtaat
    13561 gccattgata ttgcactacc tggtgtcaca ggaggctcgc cacgctacac agaagcagcc
    13621 aattacgcat ttgagaagtt tgcaaacaaa atcggctatg ttatcacaaa tggcaaggtt
    13681 cgtgaccgtt caggacaatc aggtacaggt attcacaatg attggagacc atggcccgat
    13741 ggagatcact atgatcatgt gcatttaaac ggtgtgaaag acccacaaaa cactcaaatt
    13801 tcaggagata gcgtgggagg cagtggggta gaaagatggc gcaatgtagc aattagagcg
    13861 ttgaaaatga ccggtcaata cagtactgca aacttaaatg cattactaaa tcaaatgcgt
    13921 acagagtcaa atggtaatcc taatgcagtt aacaattggg atattaacgc caaaaatgga
    13981 acaccatcaa aagggttgct ccaagtgatt gacccaacat tcagacagta tgcaatgcca
    14041 ggattcaaca gcaatatttt tgacccacta tctaacatct tagcttcaat cagatacgca
    14101 ctatcaagat atggctcact aacaaatgcc tatcgtggag ttggttacgc aaacggtgga
    14161 attgtaaacc aacatcaaat tgcggaaatc gcagaaggaa acaagccaga aattattatt
    14221 cctttagata aggctaaacg atcaagagcg atgcaattat tgtctatcgc aatggacaaa
    14281 ctaggagttt ctcctaaaac ttatggaaat gcgacaactg tttccagtga ttacgaaatc
    14341 ctagaagcca ttgaaaaaca ggcggcaaca acgaatcagc tgttatcgtt attgctggca
    14401 tttttcaaag gaaatagccg aactgataga gatttagctt tagatattca gaagatcttg
    14461 gttaggagga tgtagatgcg aactgtatta ctgagaaata gaataaacga agaaattgat
    14521 ttgtctacgg aggattattt tgctactggg ttgagtaata tggggtttga agtaaaaaaa
    14581 gaacatgtgg gacagtgggg gaatttcaga gaaagcagtg agaacgttga aatatctgaa
    14641 tttcagtcat ctgtaatcat ttctgtgcat gggtttcgag aaaaagaact gtacaattca
    14701 cttgtgcagt tcctatcgga aggtccgttt gaactggaat ttgcttttga cggcgaaaca
    14761 atggtaagaa gatgcagtct aaaatcttta agtaagactg aaatcgatcc gaaaacatcg
    14821 ttactaacag acactttaga actatatttc acatcaaact ggtattccgt aaaacgcgaa
    14881 aaattaattc agcgaccgaa tgtagtgaaa acacgtggta aggtttttcc gtataaaaga
    14941 tcttatatct acactcaaaa cttgtgggag aaaaaaggtg tattcaaatt caataataat
    15001 tctgtatacc taacgaatag caaggaacgg atgtccccgc taaaaattcg tgtgattggg
    15061 aagtgttcaa atccgtattg ggaagtaatc caaaactcac aaatcatcgc aacggatgga
    15121 tacttcatag atatgactga aacacagact ctagaagtat caagcctttt tgaagataca
    15181 acggcgattt taaaagatat tgcaggggta gaatcttctg tctaccaaca acaggattat
    15241 acaaaaacta attttgttca ggccccgact ggagaattca gcattgtgtt tcatgttggg
    15301 ggggcagacg tagagattga gttatacgag gagcgtgatc tgttttgatt ttagcagtca
    15361 cgctttttca tcgtgattta atgctttata acgaacattt attctctaac agttttgaat
    15421 ttggtgcgga cgagatcaac gaagaggcta gtagctttac gatggataag tacgttcctg
    15481 taaaaacagg cgattttcta ctagcaaaat atattcctag tggaaaattt gcatattttg
    15541 gtgtaattac gtcgcaggaa gacgaaaaaa tcagttgtaa aagcttactt agtttagctg
    15601 atagtgagat accgactgca cgtgtgtcag gagataacta cgaagagcat atccggcggt
    15661 tgattgaata ctatttgctg aatgatccaa cgaaacaact aaaagatatt ttagacgtca
    15721 aagcagaaag tgcgacctct cattcgtatc aagctaccga tacgaataaa cacaagttaa
    15781 gtgcgtatat tctcaatggt ttcaaaaaat acaacgtaaa atggtatttc aagggaattc
    15841 aaaacagaaa aatctataca ggcatacgtg ctgtaaatga atcaatttac attaaagaca
    15901 attcttctga atttagcgat tgggatgtgt ttgttcaggc gccgggcgct ggaaacgaga
    15961 acaagctatt aatcgttgat aaagcaatga aagatataga gaaaccgata atactgtcaa
    16021 catggtattt ggacgaagaa aacaatttga cacaagatgg atcgaaagaa aatatcacga
    16081 aaccaactgt caatttggtt aatatctacg atcaaaccgc agaagataaa gcatcctacg
    16141 aagatgtggc aaaatcagag ttgaaaggca atacgtattc gcatgaaatc aaagtgaacg
    16201 ttgtaagaaa tgcaaaaaat ttgaatgtcg aaacgattga aacagggatg tttgccacga
    16261 tttcttataa aggaaagata tacaagtcgg ttttgacagc ttggcgaata tcaagtgata
    16321 aggagtttgt ggaattgact ttcggaaaca taagaagtcg ttttatggat tattttgaag
    16381 ataatggggg ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag
    16441 taagcgcaga aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta
    16501 ttgatggtta cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg
    16561 caggagcagc gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag
    16621 ttccagcaaa ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac
    16681 ctggatctat tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag
    16741 aatttataac aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac
    16801 cactatgctc agttacttct actggatcaa ctgtttcaat ctcaaagata tcggatagtt
    16861 acgagctgac tctctctaaa ggagaaattt tgtggagtgg gactgcgtta atgcatgata
    16921 ctcaaactgt ccaaccttca aaaaaaatgt ggcagacagt ttcaggtttt ttattagtat
    16981 ggctcccata cgaaaacggg caagcaattg gggatagata tgtaactacg cctttttata
    17041 aagagcgtgt aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc
    17101 acaaaaagtg gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg
    17161 cagcaagcaa tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt
    17221 agttaggagg tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca
    17281 gccttatcta cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa
    17341 tgtacaactt cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt
    17401 gaaatttgtg ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga
    17461 ttcgactaat gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca
    17521 attcaaacaa gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa
    17581 cttcatcata gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat
    17641 ttcttcattg gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga
    17701 taaacaggtc caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa
    17761 tgaatcagat aagcaagtaa atgatcgtat tgataagaca aatgatcgta ttgatcaaac
    17821 caatcagcaa atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcatcgactt
    17881 cggggactat gattatagtg ggaatccgaa ccttagcgct aaacttaatg cttcaagttt
    17941 ttctttaggt actggcgcaa cagtcgcgga tgataatgat gagattgtgt ttaccttaga
    18001 tggcacaaat caactatcga agtacgctag caaagcgcaa gtgccggtag cagaagggaa
    18061 acaatatacg attagttgtg aaattatgtt agaagatggg ttcactggcg acccttcagg
    18121 aatacgtctg caacacgcat atttgccagg cggggttgtt gttttacaga cagagacagt
    18181 acctaaaaat gaattgaata cgtggcagac gcttatcgga acgcgaacgg taaaatacac
    18241 ctctgacgta ccaaatgaat ggtacccttt gtttagagac atccgaacac ttaaaccgac
    18301 cggtaaagtt agactaagaa aaattaagat tgaagaaggc tcaacagcca ccccatatca
    18361 gccaaactta cttgatgacc cttactggct aggtaaagcg cctttgggtg agaatattgc
    18421 taataagtct gttacgtttc caatcaaatc tagcgcctac agcctatacc aagctaacat
    18481 ggaagaagaa tttgttttag gtcaaaccta tacaattacc atgaaggcaa ccaaaccacc
    18541 tatccaaacg ttcattgttt acaacgagga tagtggagat tatagatatg gtaacttaga
    18601 gccagtagaa gggttagttg atacatgggg tctgactttt acaccgcaga aggttggagt
    18661 taactacccc aaacggttaa ctattatcca gtatccccaa tcaacaacag gtgcatgtca
    18721 aattgactgg ctcaagatcg aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa
    18781 atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt gggatctagc
    18841 accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa ttactggtaa
    18901 agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat
    18961 taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc
    19021 tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt
    19081 aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc
    19141 tatatatttt gtggctttaa acggaaaaag cagtggtgga aatgattcaa cgatactaac
    19201 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
    19261 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
    19321 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
    19381 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
    19441 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attctaaatt
    19501 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
    19561 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
    19621 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
    19681 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
    19741 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
    19801 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
    19861 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
    19921 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
    19981 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt actgacgatc
    20041 aagttcgact attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
    20101 aagaagaaag cgcatcttaa gtgatgcgtt tttatttaag gtaaaggagt tgtcacatga
    20161 tcaatttagg ggaatgggga acaatcgcag gatcaattac tgcgattgtt tctttgattt
    20221 tattagtaat aagaccagtc actgcatctt tctcgaagat tactgaaact ctttcaaaag
    20281 taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
    20341 tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg
    20401 tagaacacac acaacaaatt aaaactttat ttagagaaag gtctcggtaa aaaagaaagg
    20461 agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg gacagtttta
    20521 acagtattgc cagctttatc tgtattagta gccacattag gcaaagcata tggatggaat
    20581 ggaacagata tggcagttct gactatcaat gcagcagcaa catttttagg tgttatcact
    20641 ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat
    20701 tactgcgatg agcctattaa cggctctttt tttattgccg attaatgggt ttgcctatac
    20761 gattaacaat gaatttaatt taggtgtaaa cgaaggtagt tctcaagtag caaataatca
    20821 gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta
    20881 tatgcaacgt tcatggacta gcgcttatac tgcttatatt gtgggagacg gcggaattgt
    20941 ttatcaagtc ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa
    21001 tagtcctgtg cagattgagt tacaacacac acatgataaa gcaacgtttg agaaaaacta
    21061 caaggcatac gttgaattag ctagagattc agcaatgaaa tatggtattc cacttacact
    21121 agacacaccg tataatcaac cagggatcaa atcgcattta tgggtaacac aaaatatttg
    21181 gggcgatcat acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt
    21241 agcctatgat ttggctcatg gatttaccga tgaaaatccg acaacttcag atgataaacc
    21301 agtcattgat ccaactcgag caggtgctgc aaatcctacc ctgacagatg gaacaaatta
    21361 cgcccacatt gatcagttcg gagaaatcga aaacgcaaac ttacatgtgg ctggttggca
    21421 catcgctaac tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt
    21481 agctcgagta aaagctgatg gaatatatag accagatgta aatcaagctt ataatacttt
    21541 aggaaatgtt ggctatcatg tatcttttaa catgcgcaac ttccctaata agaaagtcta
    21601 tgtaatgatg cgggcaacga atgatccaga agggaacact aaaggcggag cacaagattt
    21661 tcatgataaa cgctggtatt taaatattcc gcaacgataa aaatagcccc tcgtttttga
    21721 ggggaagtac ataatgcaaa tttgtccata acttattgat tatcttgtaa aaatgcggta
    21781 atttaatagt tagtgagaag aggtgcttaa ttatagttaa aaaagaggaa atagaagcag
    21841 catgtaacca agcaaatgtt aggtttattg gagaagcttt atctggatat cctaagtata
    21901 aatacttaca caactggtta caaagtaaaa gtttttatct taataaagaa a
//
LOCUS       DADVXO010000039        21787 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.040, whole genome shotgun sequence.
ACCESSION   DADVXO010000039 DADVXO010000000
VERSION     DADVXO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.040"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            29..583
                     /locus_tag="KB950_001235"
     CDS             29..583
                     /locus_tag="KB950_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6857464.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(756..1229)
                     /locus_tag="KB950_001236"
     CDS             complement(756..1229)
                     /locus_tag="KB950_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6857465.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1241..1777
                     /locus_tag="KB950_001237"
     CDS             1241..1777
                     /locus_tag="KB950_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6857466.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1817..2188
                     /locus_tag="KB950_001238"
     CDS             1817..2188
                     /locus_tag="KB950_001238"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6857467.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2472..3743
                     /locus_tag="KB950_001239"
     CDS             2472..3743
                     /locus_tag="KB950_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6857468.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3988..5013
                     /locus_tag="KB950_001240"
     CDS             3988..5013
                     /locus_tag="KB950_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6857469.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5307..6416
                     /locus_tag="KB950_001241"
     CDS             5307..6416
                     /locus_tag="KB950_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBB6857470.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6499..7395
                     /locus_tag="KB950_001242"
                     /pseudo
     CDS             6499..7395
                     /locus_tag="KB950_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7411..8235
                     /locus_tag="KB950_001243"
     CDS             7411..8235
                     /locus_tag="KB950_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6857471.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8267..9577
                     /locus_tag="KB950_001244"
     CDS             8267..9577
                     /locus_tag="KB950_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBB6857472.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9725..10984
                     /locus_tag="KB950_001245"
     CDS             9725..10984
                     /locus_tag="KB950_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6857473.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10994..11866
                     /locus_tag="KB950_001246"
     CDS             10994..11866
                     /locus_tag="KB950_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6857474.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11878..12708
                     /locus_tag="KB950_001247"
     CDS             11878..12708
                     /locus_tag="KB950_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6857475.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12748..14931
                     /locus_tag="KB950_001248"
     CDS             12748..14931
                     /locus_tag="KB950_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6857476.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14992..16212
                     /locus_tag="KB950_001249"
     CDS             14992..16212
                     /locus_tag="KB950_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBB6857477.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16209..17669
                     /gene="gtfA"
                     /locus_tag="KB950_001250"
     CDS             16209..17669
                     /gene="gtfA"
                     /locus_tag="KB950_001250"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBB6857478.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17746..18852
                     /locus_tag="KB950_001251"
                     /pseudo
     CDS             17746..18852
                     /locus_tag="KB950_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19174..19383
                     /locus_tag="KB950_001252"
     CDS             19174..19383
                     /locus_tag="KB950_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6857479.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19376..19525
                     /locus_tag="KB950_001253"
     CDS             19376..19525
                     /locus_tag="KB950_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBB6857480.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19679..20344
                     /locus_tag="KB950_001254"
     CDS             19679..20344
                     /locus_tag="KB950_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6857481.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20628..21713)
                     /gene="xerS"
                     /locus_tag="KB950_001255"
     CDS             complement(20628..21713)
                     /gene="xerS"
                     /locus_tag="KB950_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBB6857482.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7397 a         3288 c         4202 g         6900 t
ORIGIN      
        1 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
       61 tgaagagata caagattttc agttttggct aagaagaaca agtaatccgg aaagagatag
      121 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
      181 agttggagat atacgaggca aaagtaagcc tattattgtt gaacataaaa caggtaagcg
      241 aaaaccgatt tttatagata atttgagaga agaaatttta ttatatactg aaggtaaaga
      301 agaaaatgat tggttatttc cgagtcggca acaaggtcga catataacaa gggatcgtgt
      361 ctatcagata tatgcagata tagcagaaaa attaggacga gatgatatag gaacacatac
      421 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
      481 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
      541 tgaaatagga gcatcacttc gtggatttaa actaggtgta taatttaaaa atttttataa
      601 acagtaaaga caaagattaa aagctatttt tatctagaac gattttattt cataaagttc
      661 gcggaataaa agctggggtt actggtactt ggtataggtt cgtgttatat aaactaagta
      721 attaaataac atttttaggt tggaaaagga tgaaattaaa aaaagagaat caatacggtc
      781 tcaaaaacgc aaacgctgcg gaataagaaa aatactgata actcaacgtt tagcagacta
      841 tctaataggg atagtctgct tttttcgttt ttgaccatac ttttgacctt ctgcaaactt
      901 ccttgctttt cctctgtaga actcatgaaa tctgcaaaac gaattgccgt ttcctctgtc
      961 tgctttttcg cgatatgggt gtatatatca tccatagtgg ttttaatatc tgtatgtcca
     1021 agtcgaacat acacttcttt gacagacgca ccagattcaa agagcagact acaatgggta
     1081 tgacgaaaac catgaagtgt gatatgtttt aagttattct ctttaatgat gattttcaaa
     1141 ttgtgattta catagtctaa ataaaggggc ttattttctt ctgatgaaaa caaaagttgt
     1201 tcaccatctt ttataggttt gattctcatt tttagatatt atgcccgcac gtattcatca
     1261 catttctatt gtgaatcgct ttattcgacc gacatttgac ttttatcata atatactcgg
     1321 tttaaaatta ctaatgaaaa caattaatca agacgatcat accatgtatc acctgttttt
     1381 ttcagataat caccaccgtg tgggaactga actgactttt tttgaagtac aagaagggaa
     1441 caaccagttt tttggaacca atacgattga acgaacgata ctaaaagttc cttccgaagc
     1501 atcattgcat ttttgggaaa tatattttga aacacaaggc gtttgccatt atggaatcga
     1561 atcattttct ggacgaccta tcttacgttt tgaagggcca gatgcaacac gattagcttt
     1621 agttccctta cgcgaatttg aagatattga tgattatttc ccgtatgtca cagaccagat
     1681 ccctacagag catgccattt taggaattga tgccattcaa ctacgcgtac aatattccga
     1741 tgcgactgaa cgagagttat tgtcggtagg ctggtagcac aaagaatacg tcagtttctt
     1801 ttcgggtcat caacgagtga caatcttaga aaatcaagac actcagttct atcaagaagt
     1861 tcagattatt caagatcgtg agaatccagt tgccattgaa gggattggta gcgtccatca
     1921 tgttgcgttt ggcgtagaaa ataaatctga tttacaacgc atcgacaaac aattacaaga
     1981 gcgcaatttt ataaattcag gaataaaaga tcgtgaattt tttgtatcac tttattatcg
     2041 tgacccaaat caattattga ttgaaattgc tacaggggaa ggaaacctag atgcaaaagc
     2101 gtatgaaaat caaagtccac gctttgaaga aattccttta tttttaccgc agtacttaaa
     2161 tttcattcgt gaacaagtgg agcaataatt agctaatcaa atccatgacg aagagtaaga
     2221 ggttgactca taagtcaaaa aataaagaaa aacgcatcaa atccattcta aagaatgttg
     2281 atttgatgcg gttcctattt ttataaacta tcattttcga ctaataaaac tacttatgag
     2341 tcagcctctc tttttttctt gatttttata cctctttcta ggtaaactaa acatctgcca
     2401 aaaataccgt gtctcattga aacattctca actatttcaa aataaagggg agtaccaaat
     2461 gaacgagtat attgaaatta tccacgcaag agaaacaact taaaaatgtc agtctcaaaa
     2521 ttcccaaaaa taaaattagt atttttacca gcgtatctgg ttcaggaaaa tcatcgattg
     2581 tttttgatac tattacccaa gaagctggtc gtcaactgaa tgacacctat gatagtttta
     2641 cccgtttatt tttacctaaa tacagtcgtc cagaggttga tgagattgat aatctttcta
     2701 cggcgattgt ggtcaatcaa aagaagattg gtggcagtgc gcgctcaacg ttagggacga
     2761 ttactaacat caatgcgtta tttcgtgtgt tgttttcatg ttttgcaaca cctagtttag
     2821 gatatgcaaa tgcttattca tttaatgatc ctagtggaat gtgttctgtc tgtcagggaa
     2881 tcggtaagac gattgcattt ttaataggat tgctttattt ttcggctatt gataatgctg
     2941 attttgtatt ttctgatcca tttaccgcag aagttttgtc tggaattgag gaacaggcaa
     3001 agaatcatgg ttattttgta ttggtgcata atgttaattc agcagaagat gttaaaacta
     3061 ttcagagaaa ttggcgtttt gaggggttca ttgttgttgg tgtacgtgta aaagaatttt
     3121 tggaattaaa taacgttttt gatggtcctg ttgtttatat tgatacgcat ctttcaaaag
     3181 agacattaag agaacttgaa caacaaatga atcgtttatt tgtaaataca aatgattacc
     3241 aagctagtac attggcagtg gaacatttga ttcattatgg tcatgagaaa atagcgtttc
     3301 tttcttttga ctacattgta ggtgaaccaa gtgttattca agaacgacat caagcttata
     3361 tcgatacatt aaagaaatat catttaacct ttaaatcgca ttggatatat aacaactctg
     3421 aatttgagcg aatttatcaa gaattagctg aatttactgc aattgttgtt actggagatt
     3481 atttagcggc taaattagta aaatttttaa aaaataagaa taaacgagtg atggaacaat
     3541 tatcgattat tggatttgat gatattactt ttgcagaatt aatggatcct gcgttgacaa
     3601 caatacgact aaatccaggt aacaaggggg cgattgcttt tcaccaactg attggactgg
     3661 ccaaacaaga agaaataaaa aatcaagtac tactattaga gggagaattg atttctcgag
     3721 actctgttca caaaatcaaa taaaaaagct ctgtgaaata agtatagtta acaataaaat
     3781 atccgaacga tagagagaat gaactgtgat ctagcagttc ctcgtcgcaa attcactaga
     3841 attagcatta tttactctaa attgttcgga tattttttct tgtttttaat tatgattaca
     3901 gtgcctattt ttaacgaaat ttgcttattt tcaaaatgta tgattatatg ttagtaaaca
     3961 ctttcaaata tgaaaagagg agctattatg gtaggaatta gggatgttgc aaaacgggcg
     4021 ggtgtgtcca ttgcaacagt atcgcgcgtg ctaaatgagg atccgacact ttcagtaacc
     4081 gatgaaacca aacgaaagat tgcagaatcg gttaattttt atcattatgt caaaaaaagt
     4141 cccgttgcaa aaaatttaac aaatattgca ttaataacga cagtttctga agtagatgag
     4201 ttggaagatc cctattttcg aacgattcgc agagggattc aagtcgaagc agaacaaagc
     4261 aaagtcaatt tgaagaaaat tattcgtttg tcagaaacac cgttggagca agacgatttg
     4321 ctaaaatgtc aaggtgtgtt gattattggg caggtgctat cttctgtgat tgaagaagta
     4381 tcagcaatta attcgaatgt cgtagtgatt gatgacccta gtattgaagc tgaagtagat
     4441 gcagtttata ctgatttaac aaaagcaacg aatcagcatt tagatcgatt gtataaaaaa
     4501 ggacaccgaa acattgcgtt tatcggagga ggacgtgttc gtttagatag ccaaggaaat
     4561 aaacacgtgt cagatgatga ttatcgaaaa atagcctatg agacgtggat gaaacaaaaa
     4621 ggattagaaa aatatagtca aatttattta gaaggctgga ccacacttga cggaatgcag
     4681 gcagcagatt tacttttgaa aaaacatgct aaagataagt tgccgactgc tatcatggta
     4741 ggaaatgatc ctattgctgt gggggtttat cgttcacttc aaaaaaatgg tctacatatt
     4801 ccagaagata tttcgattgt gagttttgat aatattgaag ttgctgaatt tttaacacct
     4861 gcattaaata cggtgaatgt gaatacagaa gaaattggaa aactagcagt tcgcatggtt
     4921 aatgaacgaa taaaagaagt tagaaatgtt tcgatcaaag tgattgtttc taataatatt
     4981 attgttagag aaagtgaaaa aaatatcgat taaaaaagta aacatccaaa aaagggtgtt
     5041 tacttttttg tttactaata tttttagtaa aaaatctcga caaaacatta agtaaacagt
     5101 tatggtgttg ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag atgaaacatt
     5161 tcaaaaaaat agctttaaca gggatagctg ttttaactgg gacattactc gcagcatgtg
     5221 gtgggggaga tagtaattct aaagacgggc aagtaacttt aagctatgcg agttgagata
     5281 ctgaccaagc tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa aatcctgata
     5341 tcaaagtaga aatggagaca acgccttggg atcaatattg ggttaaatta gaagctgcaa
     5401 caacgggagg taatatgcca gatgtagtaa ccatgcactc tagcgaatcc tataaatata
     5461 tgtcacagaa tgctttgatg ggacttgatg atgttatcaa agaaaatgat attgacatgg
     5521 gaaactttac tgaaggaatt gctgatttct atacgcttga agacgaacta tacgcaatcc
     5581 ctaaagatgc ttctgtagtg gggctatggt acaacaaaga attatttgat aatgcaggtg
     5641 tcgattatcc agatgaaaca tggacatggg aaacactcag acaagctgca attgatttaa
     5701 cagatgaatc aaaaggcatc tatggttttg catcacatat tggaacagag actggctact
     5761 ggccatttat ttatcaaaat gggggagaag tatttgccga tgacaataca aaatcaggtt
     5821 tgaatacccc agaagcacaa gaagctttag ctttttatcg agatattgca cttgttgatc
     5881 atgcttcacc aacaattaca gaattacaag atcctgataa gttaagtcgt tttcaatcag
     5941 gtagattagc aatgatgatt gaaggaaact ggtttgcacc gggatttttg aaaaatgact
     6001 atctagttga aaatgttgct tctgccgtgt tacctaaggg aaaagttaat gcaacaatta
     6061 caaatggact tggttgggct gccagtgcga gtacaaaata cccagaagaa gtgaaaaaac
     6121 taatggcatt tttagctagt gaggacgcca ataaaatcca agcggaaacg ggggcatcaa
     6181 tcccagcact aaaaggcttt gaagacgagt gggctgcttc ttcagataca attgatttga
     6241 gtgcttttgg aaaaatgttg gagtatggag tccctcgtcc atacaataaa gatggattac
     6301 gagctgaaca agcagaagtt caaattatta accgtattct gtctggtgaa gtaactgttg
     6361 aagatggaac cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga aattaaatta
     6421 gttaattata caatatacag gcgaaaggat tctatttcgt ctgtatatta atggatgaat
     6481 accaaaaagg gggatattat gaaaaatcga atttttggaa aatatcgtaa agatcaagtg
     6541 atatggggct atatttttat ttttccaacg gcattaggat taattttatt aaatatatgg
     6601 ccagcaattc aaacagcaat tttaagtttt caaaaaacgg tagggttcgg tgatactgaa
     6661 tgggtcggat taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt tcagtcttta
     6721 ttaaacacat taatttatgc agtggtttcg atacctagta ttgttgtatt atccttgttg
     6781 gtggcagttt taatgaatag aaaagttaaa ggattgtcga tttatcggac aatttatttt
     6841 ctaccagtcg ttgctgcacc atccgcagtc gcaatgattt ggcgctggat gttcaataat
     6901 gattatggac tgattaataa catgttggct aaaataggtc tcgatggacc agcatggtta
     6961 acggatccat cgattgcgat ttattctatt atcattgtag gagtttggag tgctattggt
     7021 tataacatgg tgttattact agccggctta taagaaattc ctaaagatta ttatgaagca
     7081 gcggaaattg atggtgctag tccaatccag cagtttttca acattacgtt accattagtt
     7141 acaccaacat tattttttgt agttgtaaca acagttatca atgctttcca agtatttgat
     7201 gttattttta tgatgattac tcctaatagt accgccatgt ttaaatcaca atcattagcc
     7261 tatttattct ataaacattc atttatttta aatgacaagg gatatggttc ggcaatcgtt
     7321 atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt gcagaaaaag
     7381 tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc aaatgcgcca
     7441 atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat ttggatgatt
     7501 ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat tttccctgag
     7561 aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat ggctttttat
     7621 atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt ttcagcaatg
     7681 gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt cagcttagta
     7741 cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt attagcacaa
     7801 aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt tagtgcgttt
     7861 ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga ggaagcggcc
     7921 tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc gcttgctaaa
     7981 tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga tttaatgtgg
     8041 ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt agcaaactta
     8101 caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt agcaatttgg
     8161 ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat tgctacaaca
     8221 ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga caaaaattac
     8281 atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg cgatgttaac
     8341 tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac gactaaaaga
     8401 atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg ctgatattgt
     8461 ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca atgctattca
     8521 agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa aatatgggtt
     8581 gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc tacggacact
     8641 accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg cttggttatt
     8701 gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga cgagtattaa
     8761 gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg agcatttagg
     8821 aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca ttaatcatat
     8881 ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa ttcgtcagtt
     8941 agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt tgaaacgttt
     9001 tggttactat attacagaat caagtgaaca caatgcagaa taccatccat actttattaa
     9061 atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat acttacgtcg
     9121 ttgtgaacaa caaattaatg attgggaaag catgcgagag gacattgtaa ataatgggga
     9181 gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta ttacgacagg
     9241 aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa acttaccgac
     9301 agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac caacctatgt
     9361 cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg ttcaagaact
     9421 gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag cgtatttaga
     9481 tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg atgatttaat
     9541 cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa gagtaaggaa
     9601 ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca aaacacttag
     9661 taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata aggaggagca
     9721 taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag caacgatctc
     9781 tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa tagagttttt
     9841 taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt ttgaagcaaa
     9901 aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag taattaaaac
     9961 acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa gtatagaatt
    10021 gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt atttagagaa
    10081 tatcaaaaat ggatatgcac aacgttttgc tattgaggat cgtgtatata gcataccatt
    10141 aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa tggggattca
    10201 agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag atcaaaacaa
    10261 agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc accaactagc
    10321 tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt tctcagaagt
    10381 taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat tagatttgtt
    10441 aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg atacagtagt
    10501 tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa tgccgatgat
    10561 tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag atgaagcagg
    10621 tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa cttctcaaca
    10681 taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga tgcagaaata
    10741 ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata ctcaggattc
    10801 accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt ggttagccaa
    10861 agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac acaatggcaa
    10921 tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag ctgacggaag
    10981 gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa tcatttttgt
    11041 agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc aaggtgcttt
    11101 ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg gtttgaacaa
    11161 ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca ctttaattgt
    11221 gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc gggcgttgaa
    11281 cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag cggtcttatc
    11341 gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac caaaaattgg
    11401 tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat ggggagcgat
    11461 ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca tcattttctt
    11521 agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg atggggcgaa
    11581 tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta tttcaatggt
    11641 atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg ctttaactgg
    11701 tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt atgcttttaa
    11761 aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat
    11821 tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg
    11881 aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg
    11941 attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg
    12001 aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg
    12061 gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct
    12121 gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa
    12181 gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt
    12241 atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc
    12301 ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa
    12361 ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca
    12421 acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt
    12481 aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct
    12541 aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat
    12601 ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct
    12661 ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat
    12721 tctcattata aaggagttca taaacttatg attatatttg acaatcacac acaaacattt
    12781 catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg
    12841 catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca
    12901 gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca
    12961 ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt
    13021 aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa
    13081 ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa
    13141 acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca
    13201 atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa
    13261 acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag
    13321 ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat
    13381 acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt
    13441 gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta
    13501 gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc
    13561 attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa
    13621 acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat
    13681 catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg
    13741 ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt
    13801 gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc
    13861 ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct
    13921 tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg
    13981 tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca
    14041 ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca
    14101 agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg
    14161 attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg
    14221 gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta
    14281 gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt
    14341 cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat
    14401 gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg
    14461 ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt
    14521 cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact
    14581 tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt
    14641 ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg
    14701 atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa
    14761 gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa
    14821 atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca
    14881 gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa
    14941 tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat
    15001 ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa
    15061 gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat
    15121 gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat
    15181 gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa
    15241 gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt
    15301 cctaatgaac gtttagatga atatattggt gaagatggct attattcgat ggtttttgat
    15361 tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg
    15421 acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt
    15481 tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca
    15541 gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga
    15601 ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca
    15661 ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga
    15721 ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca
    15781 cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta
    15841 aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg
    15901 gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg
    15961 ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa
    16021 cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa
    16081 tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta
    16141 gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga
    16201 ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa
    16261 aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg
    16321 tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata
    16381 cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt
    16441 atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt
    16501 tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt
    16561 tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag
    16621 cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg
    16681 gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa
    16741 ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg
    16801 cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag
    16861 acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc
    16921 attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc
    16981 caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga
    17041 aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg
    17101 acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag
    17161 tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc
    17221 aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac
    17281 gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag
    17341 gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt
    17401 actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg
    17461 cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag
    17521 caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg
    17581 caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa
    17641 ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac
    17701 tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa
    17761 acaaatacag catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac
    17821 tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg
    17881 ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca
    17941 gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa
    18001 ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg
    18061 ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat
    18121 tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa
    18181 tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat gtatccaact
    18241 gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat
    18301 caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag
    18361 atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg
    18421 atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta
    18481 ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga
    18541 cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg
    18601 tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt
    18661 aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt
    18721 tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa
    18781 atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta
    18841 attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag
    18901 ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt
    18961 tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt
    19021 tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata caaagattta
    19081 taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta gtagtgttct
    19141 tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa aaatgaatta
    19201 atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc aaaagcaatt
    19261 atggcttcaa aaagctatac ctattacgaa aatagaagat taggactagt acctgttgaa
    19321 tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca gaatcatggt
    19381 taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg gattcgacaa
    19441 acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa agaagaaaaa
    19501 aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct caaagtacta
    19561 tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg aaagtagaag
    19621 aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt tctttaaaat
    19681 ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt tattgagcaa
    19741 atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat tagatcgagc
    19801 agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg tgaagaaaag
    19861 gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt caacttttta
    19921 tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt ttatgaccgt
    19981 tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg aaaataaaaa
    20041 cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt ttgtagaacc
    20101 cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta cctttttaaa
    20161 agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt cttataatgg
    20221 aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa cgatcgttgg
    20281 tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc ttatatcaca
    20341 ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac aaacgacttt
    20401 aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg atcccacaga
    20461 ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata ataaaaaata
    20521 aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact ataatacagg
    20581 cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca taaagcattt
    20641 aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt attggtactt
    20701 gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta gagtctagta
    20761 gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt tgaatatttt
    20821 gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg gtaaattgct
    20881 agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat agctagataa
    20941 ttagctaaat attccattgc gaatgctgct atattgacag catctctttt atttcctttt
    21001 ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat atttataacc
    21061 tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc tctttctaca
    21121 tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc attggcaata
    21181 taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat ggcattggca
    21241 cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg attattgtct
    21301 aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa tagaaccttt
    21361 aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt attatctttg
    21421 agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt caaatgagat
    21481 ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga agtatcagga
    21541 acgatagact cactcagaag ccattcataa aagagcttat aattttttag atattctaaa
    21601 gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt tatataatcg
    21661 ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt catgtcattc
    21721 atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc aattatagaa
    21781 tgttttg
//
LOCUS       DADVXO010000040        21438 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.041, whole genome shotgun sequence.
ACCESSION   DADVXO010000040 DADVXO010000000
VERSION     DADVXO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.041"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            119..820
                     /locus_tag="KB950_001256"
     CDS             119..820
                     /locus_tag="KB950_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6857483.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="KB950_001257"
     CDS             complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="KB950_001257"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBB6857484.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="KB950_001258"
     CDS             complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="KB950_001258"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBB6857485.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="KB950_001259"
     CDS             complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="KB950_001259"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBB6857486.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="KB950_001260"
     CDS             complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="KB950_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBB6857487.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4352..5332)
                     /locus_tag="KB950_001261"
     CDS             complement(4352..5332)
                     /locus_tag="KB950_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6857488.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5356..6840)
                     /gene="galT"
                     /locus_tag="KB950_001262"
     CDS             complement(5356..6840)
                     /gene="galT"
                     /locus_tag="KB950_001262"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBB6857489.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6852..7841)
                     /gene="galE"
                     /locus_tag="KB950_001263"
     CDS             complement(6852..7841)
                     /gene="galE"
                     /locus_tag="KB950_001263"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBB6857490.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8089..8256
                     /locus_tag="KB950_001264"
     CDS             8089..8256
                     /locus_tag="KB950_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBB6857491.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8318..10129)
                     /locus_tag="KB950_001265"
     CDS             complement(8318..10129)
                     /locus_tag="KB950_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBB6857492.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10126..10491)
                     /locus_tag="KB950_001266"
     CDS             complement(10126..10491)
                     /locus_tag="KB950_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBB6857493.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10654..11049)
                     /locus_tag="KB950_001267"
     CDS             complement(10654..11049)
                     /locus_tag="KB950_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6857494.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11067..12029)
                     /locus_tag="KB950_001268"
     CDS             complement(11067..12029)
                     /locus_tag="KB950_001268"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBB6857495.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12029..12241)
                     /locus_tag="KB950_001269"
     CDS             complement(12029..12241)
                     /locus_tag="KB950_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBB6857496.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12262..12960)
                     /locus_tag="KB950_001270"
     CDS             complement(12262..12960)
                     /locus_tag="KB950_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBB6857497.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12980..13522)
                     /locus_tag="KB950_001271"
     CDS             complement(12980..13522)
                     /locus_tag="KB950_001271"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBB6857498.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13654..14658)
                     /locus_tag="KB950_001272"
     CDS             complement(13654..14658)
                     /locus_tag="KB950_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBB6857499.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14655..15644)
                     /locus_tag="KB950_001273"
     CDS             complement(14655..15644)
                     /locus_tag="KB950_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBB6857500.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15641..16447)
                     /locus_tag="KB950_001274"
     CDS             complement(15641..16447)
                     /locus_tag="KB950_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857501.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16613..17569
                     /locus_tag="KB950_001275"
     CDS             16613..17569
                     /locus_tag="KB950_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6857502.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17646..18164)
                     /locus_tag="KB950_001276"
     CDS             complement(17646..18164)
                     /locus_tag="KB950_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6857503.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18252..18401)
                     /gene="rpmG"
                     /locus_tag="KB950_001277"
     CDS             complement(18252..18401)
                     /gene="rpmG"
                     /locus_tag="KB950_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6857504.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18629..19075
                     /locus_tag="KB950_001278"
     CDS             18629..19075
                     /locus_tag="KB950_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBB6857505.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19270..21165)
                     /gene="asnB"
                     /locus_tag="KB950_001279"
     CDS             complement(19270..21165)
                     /gene="asnB"
                     /locus_tag="KB950_001279"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBB6857506.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6450 a         4549 c         3699 g         6740 t
ORIGIN      
        1 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
       61 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      121 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      181 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      241 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      301 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      361 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      421 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      481 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      541 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      601 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      661 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      721 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      781 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      841 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      901 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
      961 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
     1021 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
     1081 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
     1141 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
     1201 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
     1261 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
     1321 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
     1381 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
     1441 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
     1501 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
     1561 tagttctacc tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg
     1621 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
     1681 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
     1741 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
     1801 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
     1861 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
     1921 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
     1981 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
     2041 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
     2101 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
     2161 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
     2221 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
     2281 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
     2341 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
     2401 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
     2461 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
     2521 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
     2581 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
     2641 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
     2701 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
     2761 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
     2821 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
     2881 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
     2941 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
     3001 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
     3061 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
     3121 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
     3181 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
     3241 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
     3301 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
     3361 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
     3421 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
     3481 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
     3541 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
     3601 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
     3661 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
     3721 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
     3781 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
     3841 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
     3901 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
     3961 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
     4021 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
     4081 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
     4141 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
     4201 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
     4261 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
     4321 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
     4381 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
     4441 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
     4501 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
     4561 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
     4621 aaagcgcatc aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg
     4681 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
     4741 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
     4801 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
     4861 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
     4921 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
     4981 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
     5041 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
     5101 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
     5161 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
     5221 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
     5281 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
     5341 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
     5401 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
     5461 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
     5521 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
     5581 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
     5641 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
     5701 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
     5761 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
     5821 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
     5881 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
     5941 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
     6001 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
     6061 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
     6121 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
     6181 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
     6241 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
     6301 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
     6361 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
     6421 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
     6481 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
     6541 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
     6601 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
     6661 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
     6721 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
     6781 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
     6841 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
     6901 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
     6961 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
     7021 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
     7081 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
     7141 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
     7201 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
     7261 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
     7321 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
     7381 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
     7441 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
     7501 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
     7561 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
     7621 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
     7681 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
     7741 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
     7801 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
     7861 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
     7921 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
     7981 aacattttca agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc
     8041 tcttttgttc cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt
     8101 gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct
     8161 tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac
     8221 cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa
     8281 gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt
     8341 gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg
     8401 cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt
     8461 cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat
     8521 aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt
     8581 ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat
     8641 agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa
     8701 tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt
     8761 tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc
     8821 atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg
     8881 gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt
     8941 tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat
     9001 tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg
     9061 gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct
     9121 ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt
     9181 ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca
     9241 ggttttcttt tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc
     9301 ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg
     9361 atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa
     9421 acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag
     9481 gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat
     9541 ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac
     9601 cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac
     9661 catcttttct tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa
     9721 attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag
     9781 attgctgatc tttttccaga cgattgattt cttctataat tagttgctta tttttggtta
     9841 ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct
     9901 gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa
     9961 cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat
    10021 tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt
    10081 ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc
    10141 catttcgata aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata
    10201 aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt
    10261 cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc
    10321 tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag
    10381 atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct
    10441 atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc
    10501 cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat
    10561 gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat
    10621 tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc
    10681 agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa
    10741 tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc
    10801 ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa
    10861 aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat
    10921 tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag
    10981 atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca
    11041 taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca
    11101 atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc
    11161 gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc
    11221 gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa
    11281 gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct
    11341 tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc
    11401 agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta
    11461 gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga
    11521 tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg
    11581 ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg
    11641 ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata
    11701 gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag
    11761 tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc
    11821 atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt
    11881 gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc
    11941 catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct
    12001 aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa
    12061 ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac
    12121 ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac
    12181 ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca
    12241 tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat
    12301 acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata
    12361 gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc
    12421 accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac
    12481 aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa
    12541 ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag
    12601 aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata
    12661 aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag
    12721 tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa
    12781 acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat aataagggat
    12841 ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc
    12901 ataggtccat aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat
    12961 gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct
    13021 ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa
    13081 aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat
    13141 tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg
    13201 attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat
    13261 aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt
    13321 ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta
    13381 tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt
    13441 tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta
    13501 atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa
    13561 aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc
    13621 tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct
    13681 gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg
    13741 tacttcccca cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga
    13801 accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc
    13861 agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct
    13921 tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa
    13981 tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc
    14041 tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc
    14101 ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat
    14161 cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat
    14221 tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc
    14281 taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa
    14341 gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga
    14401 atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc
    14461 tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa
    14521 agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt
    14581 accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt
    14641 ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca
    14701 atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta
    14761 aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga
    14821 tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca
    14881 ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac
    14941 cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa
    15001 gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat
    15061 actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc
    15121 tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata
    15181 gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa
    15241 ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa
    15301 tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg
    15361 attcctagta agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg
    15421 ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt
    15481 cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct
    15541 gctttttcca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca
    15601 aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct
    15661 cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa
    15721 tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg
    15781 taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag
    15841 catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag
    15901 tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca
    15961 agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc
    16021 caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg
    16081 cccactcaat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt
    16141 atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg
    16201 ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat
    16261 tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag
    16321 attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag
    16381 acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc
    16441 tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat
    16501 ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata
    16561 tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt
    16621 aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg
    16681 ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag
    16741 tactcataca gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg
    16801 gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca
    16861 gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc
    16921 aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt
    16981 gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc
    17041 aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg
    17101 caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat
    17161 caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg
    17221 ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata
    17281 cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc
    17341 tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct
    17401 tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc
    17461 agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc
    17521 tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa
    17581 aaaagcatct tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat
    17641 agattttatt cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga
    17701 tgggcatata gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct
    17761 gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc
    17821 cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag
    17881 atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc
    17941 gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga
    18001 agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt
    18061 gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg
    18121 ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat
    18181 gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa
    18241 ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat
    18301 ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt
    18361 tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt
    18421 acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt tttctagatt
    18481 gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta
    18541 cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt
    18601 atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc
    18661 aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt
    18721 agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg
    18781 gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa
    18841 gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt
    18901 agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca
    18961 ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc
    19021 aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata
    19081 tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt
    19141 cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg
    19201 aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt
    19261 tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac
    19321 aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc
    19381 tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc
    19441 ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa
    19501 ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag
    19561 atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt
    19621 acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc
    19681 caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg
    19741 atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta
    19801 atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc
    19861 tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc
    19921 aagtgattca tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc
    19981 taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa
    20041 ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc
    20101 tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac
    20161 gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa
    20221 cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt
    20281 tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc
    20341 tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac
    20401 ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc
    20461 gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg
    20521 aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc
    20581 tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc
    20641 ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc
    20701 aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct
    20761 tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat
    20821 cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc
    20881 atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata
    20941 gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc
    21001 gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata
    21061 ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt
    21121 ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc
    21181 taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac
    21241 gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt
    21301 acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt
    21361 ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac
    21421 acgacctaaa gatcgtgt
//
LOCUS       DADVXO010000041        21292 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.042, whole genome shotgun sequence.
ACCESSION   DADVXO010000041 DADVXO010000000
VERSION     DADVXO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.042"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..717)
                     /locus_tag="KB950_001280"
     CDS             complement(<1..717)
                     /locus_tag="KB950_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857507.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGK"
     gene            974..4363
                     /locus_tag="KB950_001281"
     CDS             974..4363
                     /locus_tag="KB950_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB6857508.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4366..5787
                     /locus_tag="KB950_001282"
     CDS             4366..5787
                     /locus_tag="KB950_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6857509.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5784..7661
                     /locus_tag="KB950_001283"
     CDS             5784..7661
                     /locus_tag="KB950_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6857510.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7753..8595
                     /locus_tag="KB950_001284"
     CDS             7753..8595
                     /locus_tag="KB950_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6857511.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8747..9622)
                     /locus_tag="KB950_001285"
     CDS             complement(8747..9622)
                     /locus_tag="KB950_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6857512.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9829..10860
                     /locus_tag="KB950_001286"
     CDS             9829..10860
                     /locus_tag="KB950_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6857513.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10949..11254
                     /locus_tag="KB950_001287"
     CDS             10949..11254
                     /locus_tag="KB950_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBB6857514.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11433..13448
                     /locus_tag="KB950_001288"
     CDS             11433..13448
                     /locus_tag="KB950_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6857515.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13450..13734
                     /locus_tag="KB950_001289"
     CDS             13450..13734
                     /locus_tag="KB950_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6857516.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13760..15133
                     /locus_tag="KB950_001290"
     CDS             13760..15133
                     /locus_tag="KB950_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBB6857517.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15216..17231)
                     /locus_tag="KB950_001291"
     CDS             complement(15216..17231)
                     /locus_tag="KB950_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBB6857518.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17253..18467)
                     /locus_tag="KB950_001292"
     CDS             complement(17253..18467)
                     /locus_tag="KB950_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6857519.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            18688..19632
                     /locus_tag="KB950_001293"
     CDS             18688..19632
                     /locus_tag="KB950_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857520.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19928..20872
                     /locus_tag="KB950_001294"
     CDS             19928..20872
                     /locus_tag="KB950_001294"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBB6857521.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7256 a         3903 c         4012 g         6121 t
ORIGIN      
        1 ttttcctaag agatttttca agattaagaa gtctttttta tcgatattta tcaactgtac
       61 ataagagtct ccagctttcc attcaaatgg agttccttgg gattctctca tgagatgcac
      121 aacaaaatac ataataaaga aagattgctc atatgggctt tcttcctgaa acaaattcat
      181 ctgacaatta ttcttgacca taatttcttt gatcttcttt ttttcgattg gaaatggcaa
      241 cgtgcgtctt ccgtagtaac ttaaaaagaa aaaggtaaca aagatacgca actggagctc
      301 acttttaagg ctagttaact gaaaaccttg gtcaacgatc acttttaata aatagctttc
      361 taaaatttga ttgcattgtt tcaacttacg tgtaaaagaa gaccggctga tggaaatttt
      421 ttcacaataa tactcaatcg ttttacccgg attgacaata atctcattta ataaacgact
      481 gtttgtagaa ctttgcgaca tttccatcag aacatttaaa taagctggta cattcaaatg
      541 ttcaagacga taccccaatt tttgtgaaga ttgaatcgtt acatccgttc cccagcggat
      601 tttgatctct tcgatatacc ttacaaatgt agattctgaa ctaccaattc gtttggcaag
      661 ttctttttta gtcatccact tgtcagaaat ggacagaatt cgaatgattt tttccatctt
      721 tctatagttt ccttccaaca acctgatcat atgtttttct ccctcaaccc ttttctttca
      781 atgaaacgct cattaatcga aggaatcaac ttcataaatg aaaccaattg gtatgaaaat
      841 tggtgtgatt ccctttagcg cccttcgtat aataattatc atattaaaat aaataaaatt
      901 atgtaacgcc tgtttatttt aataattttc actattcacg tcaaaacaga aaaaaagaaa
      961 aaggagggga aaaatgacga ccacagggaa gaaactgaaa gttattttca tgctgataat
     1021 attgagttta tcaaactttg tgccattatc tgcaatagca gacactacag atgatccaac
     1081 agttttagaa acaatttcag ctgaagtcat ttcggatcag tctggaaaaa aagcactgaa
     1141 catcaagcta aatgcgaata acaccagtgc tgaaaagata gaaaaagaaa ttggtctagt
     1201 cgaaaattac ttaagtgatg tggaaagaaa agaaggagat ggctatgctt atcaggtaaa
     1261 tagcgggaaa attacgttgg aaatctcatc aaacactaaa caaactatcg atctgagttt
     1321 tccaatcgat ccagcacttt accacagcca ggcaaacaag ctgatcgtcg ataataaaga
     1381 atatgacatt attgatgaga cagaaaataa gaaagataca gatgtgtcag taccaaagcc
     1441 agacgaaata gaagaagaat catcaaaaga aaacgaaaat tctgtcagcc catttacatt
     1501 gcctacatta tccttgccag ctgtgagtgt gccatctaat caaacgattc ctacagaata
     1561 tacaacagat gatcagggca cttatcctaa agccagttgg caacctacag gaaatacaaa
     1621 tgttcttgat catcaaggca ataaaaacgg aacaaatcaa tgggatggta taaattcttg
     1681 gaatggagat cctaatgatc ggacccattc gtatatcgaa tatggaggaa ccggtaatca
     1741 agcagactat gcgatacgaa agtatgcaaa ggaaacaagt acacccggat tgtttgatgt
     1801 ttatttgaat gctcgtggaa atgtacaaaa agatatcacg cctcttgatc tcgtattggt
     1861 cgtagactgg tcaggaagta tgaacgacaa taatcggatc ggtgaagtaa agattggtgt
     1921 cgatcgtttt gtcgatactt tagcagatag cggtatcaca gacaaaatca atatgggata
     1981 tgtcggctac tcaagcgaag gatatagcta cagtaacggt gcagtacaga tgggttcatt
     2041 tgattcagtg aaaaatcaag taaaatccat tacaccttca cggacaaatg gtggtacttt
     2101 tacacaaaaa gcactaagag atgcaggaag catgctatcc gttccaaatg gacataaaaa
     2161 agtgatcgtt ttgctgacgg atggtgtacc aacattttcc tataaagtac agcgggtaca
     2221 cgcacaatca agcagcaatt attacggaac tcagttttct aatacgcaag atcggccggg
     2281 aaatacttct ctaatctcaa gaatctatga tgcacctgac caaaacaatc tatccagaag
     2341 aatcgacagt acgtttatcg caaccatcgg agaagcgatg gcactcaaag aacgaggaat
     2401 cgaaatacat ggtcttggca tccaacttca aagcgatccg gcagctggtc tctcaaaagc
     2461 agaagtagag tctcgtatgc gacaaatggt ttcatcagat gaaaaaggcg atctttacta
     2521 tgaatcagct gatcatgcaa cagatatctc tgaataccta gccaaaaaag ctgtacagat
     2581 ctcagcaact gtaagcaatg gacaaataaa tgatccaatc gcagaaccat tcatttatca
     2641 gcctggtaca ctttcagtca agagtgtggg gacaagtcct acaacggtca ctccatctat
     2701 ttccatagaa ggaaatacca tcaagagcaa tcagatctat ttaggaaaag accaagaaat
     2761 ccaaatccat taccaagtga gaatccaaac agaaaatgag gacttccatc caaatttctg
     2821 gtatcaaatg aacggcagga caactttcca gccaaacatt gataccaatg aattagctga
     2881 attcggtata ccatctgcta aagctcccgg agtcagtctt cacatcaaaa agttatggga
     2941 agaatttgac aacaatctag ctgatcgtcc agatcaagtt acttttgaga ttcaacggga
     3001 acatacgaca aatgctgcag cttggaaaaa cggatatatt cgaatcatta aaccagctaa
     3061 agatacaaca aatacgtggg aacgtgcaga cattgacaaa ttatctgcaa atagcggaga
     3121 aagttatcaa gagatattat cactacctca atacaataat caaggtcaag cattcagtta
     3181 ccaaacaatc aaagaattac ctgtaccagg atacgattct caacaaatag atgcaatgac
     3241 atggaaaaat actaaacaat tcacaccgtt aaacttgaaa ataacgaaaa attcctctac
     3301 aggtgaaaag gatcttattg gcgctgtttt caaattaaca ggagattcta ttgatacttt
     3361 actaacagat catggcgacg gaacctattc tcttccagaa aatgtcaaat tgcaaaaaga
     3421 aatgacctat acgctgacag aaacaaaagc tccagaaggg catggattaa gcaaaaagac
     3481 tacttgggaa atcaagatcg cttctgatgg tacggtaacc attgatggaa aaacagtcac
     3541 tacttccgat gatacgatcc agttgactat tgaaaatcct tttgttgaag ttcctgtagc
     3601 agtacgtaag tatgcgatgc aagggacgga caaagagata aatcttaaag gagcagcatt
     3661 ttccctacag aaaaaagaag caaatggtac ttatcagcca attgacagcc aaacaacgaa
     3721 tgaaaaaggt cttgccagtt ttgattcact cacacctggt aaatatcgag tcgttgaaac
     3781 agctggtcct gccggatatg atacttcgcc gggaaattat gaattccaaa tcgataaata
     3841 tggaaaaatc atttacacgg gaaaaaatac cgagatgaca aataatgtat ggacgctcac
     3901 tcatcaaaat cgactaaaag cgtttgatct aacggtacac aaaaaagaag acaacggaca
     3961 gacattaaaa ggagcaaaat tcagactgca gggaccagaa atggacttag aatcgccaaa
     4021 agatggacaa gaaacagata cctttctatt cgaaaattta aaacctggaa cttatacgct
     4081 gaccgaaact tttacaccag aaggatacca aggtctaaaa gagccagtta ctatagttat
     4141 acacgaagat gggtcaattc aagtggatgg acaagatcat gaatctgttc tgtcaccagg
     4201 agccaaaaac aaccagattt ctttagacat cacgaatcag gcaaaagtac cattacctga
     4261 aacgggagga attggccgtt taggaatcta tctagtaggg atgattggtt gtgcgttttc
     4321 tatttggtat ctttttttga aaaaagaaag agggggcagc taaatatgaa aaaacttggt
     4381 tggcttagta tgtgtctctt cttgttacta tttaaaccag cttttactca ggtagcaaca
     4441 gaaacagaaa cagaaatggt tcagattact ttacacaaat tgcttttccc aaacgggcaa
     4501 ctgccgaaaa atcatccaaa tgacggacaa gaaaaagctt tattacaaac gtatcgagga
     4561 ttaaatggtg tcacattcca agtttatgat gtcacagatt ctttttacca tctacgggaa
     4621 aagggcaaaa cggtagaaga agcacaagca gagatcgcaa aaaacggtgc gtcttccggt
     4681 atgtttaccg cagaagcaac aactacaact cttaacaacg aagatggtat cgcttctttt
     4741 tctctggccg ctaaagatca agaaaaaaga gataaagcgt atcttttcat tgaatccaaa
     4801 gtaccagaag tcgtcaaaga aaaggcagag aatatggtag ttgttcttcc tgtacatgga
     4861 caaaacaatc aaaaactttc aactatccat ttgtatccta aaaatgaaga aaacgactac
     4921 cctgatccac cttttgagaa ggtattagaa gagcctagaa atgattttac gattggtgaa
     4981 aaaatcactt attccttgca tacgacaatt cctgtaaata tccttgacta tcaaaagttc
     5041 gaattgtcag atagtgcgga tgaagcatta acgtttttac ctaatagttt aacgatttca
     5101 tcgaatggag aaaagctgac agaaggcttt gtcatacaca agaaacctca cggatttgat
     5161 gttttatttt cgatcccttc gttggaaaaa tatgctggaa aaaaactgac catttcttat
     5221 cagatgcagc taagcagtac agcacaggcg aacaaggaaa tcaacaacaa cggaacactg
     5281 gattttggtt ttggtgtcag tacaaagaaa gtctctgtat atacagggag taagcaattt
     5341 gtcaaaatcg agacaaataa accagataaa cgattagctg gcgcagtatt ccttattaaa
     5401 aacaaagcag gaaattacct ccagcaaaca gccaacggat acaagtggac aaagaacgaa
     5461 tcagatgcgc ttcacctgat ttccgataaa aatggcgctt tttcaatttc cgggttgaaa
     5521 acaggaagtt atcgattaaa agagatcgaa gcaccttctg gttatatttt aagtgaaaca
     5581 gaaattccgt ttaccatttc aacttttctt tctgaggata aagaggcgga cagtatattg
     5641 aaagtagtca ataaaaaaga aaatagccgt ccatttcttc caaaaacaaa cgaaacgaaa
     5701 aatacacttt taggcgttgt tggtatggta ttcgcaagct ttgcaatctg gttgtttatc
     5761 aaaaaaagaa caggagtgaa aaaatgaaaa atcataaaaa aataaacgtt atgttaggag
     5821 tccttttcct tattttacca ttactcacaa acagcttcgg cgcaaaaaaa gtgtttgcag
     5881 aggagacagc agctcaagtc atccttcata aaaagaaaat gactgattta cccgatcctt
     5941 taatccaaaa cagcgggaaa gaaatgagcg aattcgatca ataccaagga ttagccgata
     6001 tttcattttc agtttataac gtcactcaag aattttatgc gcaacgagat aaaggagcgt
     6061 ccgtggatgc agcaaaacaa gcagtccagt ctttgactcc tggtacacca gttgcttcag
     6121 gaacgacaga tgctgatgga aatgtcactt tatctttacc taaaaaacaa aatgggaaag
     6181 atgcagtcta cacgatcaaa gaagaaccaa aagacggagt gtcagctgcc gcaaacatgg
     6241 ttttagcttt ccctgtatat gagatgatca aacaagcaga tggctcttat aaatacggga
     6301 cagaagaact agatactatc catctctacc ctaaaaatac agtcggtaat gatggaacgt
     6361 tgaaagttac aaaaatcggt actgccgaaa acgaagcact aaatggagca gaatttatta
     6421 tttctaaaga agaaggaaca ccaagcgtca aaaaatacat ccaaagtgtc acagatggat
     6481 tgtacacttg gacaactgat caaaccaaag ccaaacattt cattactggt cattcttatg
     6541 acatcggcaa caatgacttt gccgaggcat ctattgaaaa aggccagttg atcgttaatc
     6601 atttagaagt tggaaaatat aatttagaag aagtaaaagc tcctgataat gcggaaatga
     6661 ttgaaaagca aacaatcacg ccttttgaga tcctggcaaa tagccaaaca ccagtagaaa
     6721 agaccatcaa aaatgatacg tctaaagttg ataaaacaac acctcaattg aatggaaaag
     6781 atgtcgcaat cggtgaaaaa attcaatatg agatttctgt caatatccca ttaggtatcg
     6841 ctgataaaga aggaacgcaa aacaagtaca caacattcaa acttatcgat actcatgacg
     6901 ctgctttaac atttgataat gattcttcag gaacgtatgc ttatgcctta tatgatggaa
     6961 ataaagaaat cgacccagta aattattctg tcactgagca aacagacgga ttcacggttt
     7021 cagttgatcc gaattatatt ccttcattaa ctcctggcgg tacattgaaa ttcgtttact
     7081 atatgcattt gaacgaaaaa gcagatccaa ccaaaggatt ttctaaccaa gcaaatgtcg
     7141 ataacgggca tacaaatgat caaacaccac cgtcagtcga tgtcgttact gggggcaaac
     7201 gatttgttaa agtagatggt gacgttacat cagaccaaac acttgctgga gcagaattcg
     7261 tcgttcgtga tcaagatagt gacacagcga aatatttatc gatcgaccca tccacaaaag
     7321 ccgtcagctg ggtatcggcg aaagaatcag caacggtttt tacaaccaca agtaacggtt
     7381 taatcgatgt gacaggtcta aaatatggca cgtactatct ggaagaaacg aaagcgccag
     7441 aaaaatatgt tccattaaca aaccgtgtag catttactat cgatgaacaa tcttatgtaa
     7501 cagcaggaca gttgatttct cctgaaaaaa taccaaataa acacaaaggt acacttcctt
     7561 caacaggcgg taagggaatc tatgtgtata tcggtgcagg agtagtcctt ctactgattg
     7621 ctggactgta ctttgctaga cgcaagcaca gtcagattta gtctttttgc aagaattgga
     7681 aaaaggagca gcggattacg caaatgctcc tttttctttt attatcaaac agtcatcaca
     7741 aggagaacac aaatgaaacc aaagaaaagg aaacttctag atctgctgat gcttttcttg
     7801 cttctctcag gaatagggat actagcttat ccatttgtta gcgacgcgct gaataattat
     7861 ctggatcagc agatcatctc tcattatcag caacaagcgg tcaaagaaaa tgaagaagtc
     7921 atggctaaaa tccaagaaaa catgacaaag aaaaaccagc aattagcaaa agaaggtgga
     7981 aatccaggtg cagatccctt tactaagaaa aaagaacctg cgaaaaagga tagaacctat
     8041 tttgagaaac atacgatcgg tattttgacg atccctgaga tcaatgtcca tttgccaatt
     8101 tttgatgaaa cgaatactgt gctgctagaa aaaggtgcct cattattgga aggaacgtct
     8161 tatcctacgg ggggagaaaa tacacatgcc gttatttcca gccatcgagg actgcctcaa
     8221 gcaaagttgt tcacagactt accgaaactg actttaggtg attctttcta tatcgaaatc
     8281 aatggacgga cactcgctta tcaagtcgat cagatcaaga cggtagaacc gactgataca
     8341 aaagatctga gaatcgaaaa agggcaggat ttcgtgacac tcctaacttg tacaccttat
     8401 atggtcaaca gtcatcgctt acttgtcaga gggcaccggg ttccttatca tgcaaaagaa
     8461 gttcaaaaag aagtacagaa agtatccgag aacaaacgat ttttcctgta tggattgtta
     8521 gctgcatgta tactgatttt cctccttctt atatatcttt ttcgtcaatc gttcaaaata
     8581 agaaacagaa gttagctatt cctcatttct ataatatatc tattcgaatc gttccgtaaa
     8641 caaacaggct gaggtttgaa aaagaaaact tcagcctgtt tttatgatga tatcaaagag
     8701 agcctatcaa acactatctt tcttattact aaatgaggat cttttcttaa gttttccgat
     8761 attctttaag aaggcctttt tgcatcaatt cttgtttgtt tttcaagttg cggatattct
     8821 cttcaaaatt ttgagaatat atcactgaga ataacgtatt taatacgtaa tcgaaggcaa
     8881 tttgagagga gaaagtcgct actttagcaa aatcatattc ctcctgcgat ataacaagac
     8941 acatatcagc ctgtttggcc atttctgaat gctgatttcc ggtaatcagc aaggtagggg
     9001 cctctacatg cttcaaatag gtcattattc gttcataatg atggatgcga ccgctgtagc
     9061 taataaaaat ggcgcaatcc tcgctagtca aattggctgc attccaagaa gaatcactgt
     9121 attcttcagc catgatcaaa aacttattca acttgaccat tttattctgg aatttccttg
     9181 ccgtgatttg agaatcacct tttgcaaaaa gaaagattcg ctgtgccttg ttcaatactt
     9241 taccgatttg atctaaatct tcatgttcaa tctgcaattg tgcttttttt attgactgga
     9301 tcgataaatc cgccattttc tttgcaattt gctgagtgct atcttcactt gaaaaaggaa
     9361 aattaggatc aatcgtttga ttgaactgag caatcaattg atgaacttcc cgccccagct
     9421 cgaaacggaa ttctttaaat ccgctgaatc ccattttttt gcataaacga atgatagctg
     9481 aatgggaagt atacgtagct tttgctaatt cttcaatcgt catgtagact gcagcttcga
     9541 tattcctacg gatgtaatcg acaatccgtt tctctgtcgt ggtaaacaac tcttgttttg
     9601 ttagtttttc ttcaataagc atgatctgac ctccaaattc tatcttactg ataaacactt
     9661 ggttccgcta ctatcttttt gatttttatc cttactttga acattaattt caccaaaccg
     9721 aatgataaca agtgtttttc tgaacttttt taccagaata gctattataa atactttctg
     9781 cgcttttcta ggatatactg aaaacatcaa taaaaggagg caccactcat gcttacaatc
     9841 gcttatcttg gcaatggaaa gagtacaaat cgctatcatt tgcctttttc aagaaaactt
     9901 acgaacaaaa tcaatatcaa aaaaattttt tccccatctg aaccagtgtg gaaaacattc
     9961 gaagacatcg aatatactga tcagctgtct gatctttggg atgatcctga gatccaatta
    10021 gttgtcatca ctacacctag tcaatttcat tatgaatatg gaaaaatggc actcgaacat
    10081 ggtaagaatg tactagtaga gaaacctttt gcagaaacgt caaaggaagc agaagagtta
    10141 tttgcattag caaaagaaaa aggtctattt atccaatgtt accaaaacag acggttcgat
    10201 tctgacttct tgacagtcca aaaagtaatc gaaagcggaa aattagggga tattttagaa
    10261 gtagaaatgc attatgatta tttcagaccg gaagtaccag aaaatacgca tgaattttct
    10321 actgcgaaca gctatttata tggacatgct tgtcatacag tagatcaagt tctttcttat
    10381 ttcggcgaac cagatgatat cagctatgat gtacgtcaac ttttagggaa aggacgaatg
    10441 aatgattact tcgatctgga ttttttctat ggaaatatga aaatttcgat aaaatccagt
    10501 tatttccgca tcaaagaacg tccaagtttc gtcgtttacg gaaaaaaagg gatgttcgtg
    10561 aaacaaacaa aagatcgaca agaagaacat ctgaaaatgt tttacatgcc tgatcatgaa
    10621 gactttggta tcgatttgcc cgaacattat ggtgtattga cctatataga tgaacaaggg
    10681 agttatcatg aagaaaaagt aatttccgag gttggtgatt acagccgtgt ctacgaaggg
    10741 atttacgaga cattagttaa tggcgcagaa aaagtcatca aagatgaaga aacaatctta
    10801 caaatgaaaa ttttggaaga aggaatcaaa agcattcaag aatcagaagg aaaaatatga
    10861 catatttgta agaatcgggt ttgttttttg acgcataaac tgtcttattg tacgatgaaa
    10921 aagtatagaa ctactaggag gaaccaacat gacaaattat gacggtgaaa aacacaaacg
    10981 attaagaggt tcaatggccc ctaaaacaga agaagaagtg gctttagcag gaactaattc
    11041 tggtgaaaca gaagacaaag aagaatcatt agctggcaca aactctggtg aaacagaaaa
    11101 taaagaaaca gctttatctg gttcaaattc tgtagaagag gaaacaaagc ctaatgagcc
    11161 caatcacttt gaaaaagata tcgaaaaagc ggttgaagat gtcaaagaaa aatttgaaga
    11221 actgacctca aaaaataaac atgatcatca ttgatcggac gaaatcaatt cataaaagca
    11281 ggagacattt ggtcacctgc tttttttctg tatccattcg atagaaatcg ttcggtacag
    11341 tcgtaaaaaa aatccaactg taagttgtac caaacacccc tcgatttaaa agcgctacca
    11401 tactagaata taagtaggag gtgaacagcg ctgtgaacta caaaattagt tatctcctga
    11461 aaacaccaag agaaatcagt ctagaaaaag cagcagagat catcgaagta aatacaagcg
    11521 aggcgatcaa acaattaact ccttttgccg aatatgtgga tgatacccgt atccgtataa
    11581 agaaattccg agcaatcgac tgggcagaaa aatttttgga tattactcga tttgaagctg
    11641 tgtataccga acaagagcga agagatcttc tttatttgat ggtttattcg actgcaacgt
    11701 ctgtttctgt ttttcatttt caagattttc ttcaggtttc taaagggaca gtcctgtcag
    11761 atatcaaaaa gataagaaat gaattagaag tggaagattg tcagctgtcg tatactagga
    11821 aaactggttt taggctgcta gggtcagaag caatattaag aagagaagca aaaaatcgca
    11881 ctgctcgttt gatgcagtca cttgctggaa gatttgggct cacgtattta accgcaaccc
    11941 tttccattga ttattacgcc aaagttagag atttgctaga taaaacgatt tcactatcag
    12001 atcttcgatt cgcaccaaat cgaatggatg aattagctta ttatttgagt ttgacaacca
    12061 ttcgaattgg aaaaacatcc atggaactgg taaaagatgc tgaactgttg gcttctcttg
    12121 cagtcactga cattagtcga caagtattga ctggccttgg ctattccgaa catcaaaatt
    12181 cggagattat atttttctct ctttgcttag catcgatcgt tgaaggagat gtccaggagc
    12241 ctgcacttga ttttttgctg gaatgttctt cagaagtcat ccatcgcatg gaattcctga
    12301 tggccgtaga atttgcttcg tttcgtgaat tattgatgaa tgtcttttat cacttggtcc
    12361 ctgcctattt tcgtatttcc tattcttttt atttacctaa tgtgatgatc gatcaaatta
    12421 agcatcaata tgcttccatc tacgaaatga caagaaaagc attacttccg ttagaaagac
    12481 gaatagggaa gagcatccct gaagaagaga ttggtttttt cactatacta tttggcggag
    12541 agatcagaaa agcggacgca gaagaaagaa atagaaaaat aagagccgtg atcgtctgtc
    12601 caagtggcat tagctcttca ttgatcttaa aaagtgaact tcagcaatta ttcccgatga
    12661 tcttttttac agagactaat tcagctgacc gcttacacga ggttgacgaa acttcttacg
    12721 atattatttt ttctactgta cctctcacac ttactgataa gaaaaagtct ctctatgttc
    12781 tgcatccaat catgactgac ttggaaaaaa atcagctgat caatcaagta caaagagatt
    12841 ggctgatacc aggattttca atgccttctg cccaagaatt gttgtcggcg ctactgcctt
    12901 atatcgaatt aaaagaaggc gtagacgaag aaaagctcta tcaagtatta aatcgtaaaa
    12961 tgaacaaact tttagaaaag aaggaggatc aacgtcctat gctatctgaa ttattaacag
    13021 aagaaatgat ccaattatcg gatcaaccaa aaaactggca agaagcaatc acatttgccg
    13081 cacagccgct attatcagaa aataaaatag aagaaagtta tatcaaagct atgatcgagc
    13141 gcgtggagca gtatggtgcg tttatccata ttggcgatca catcgcatta cctcatgctc
    13201 gtccagaaga tggcgtaaac gaagtaggga tgtctttgct gaaacttcaa caacctgtgg
    13261 atctgcttga taatccagcc catcccatta gtttgttcat ctgtttagct gctatcgata
    13321 atgaggcaca tcttcgggct ttggcaaatt taacaaagct actttctaat aaacaaaatc
    13381 ttcaagattt attacacgca tcaacaaaag aagaaatatt agcgatcata agaaaaggag
    13441 acgaatgata tgaaaatttt tgcagtatgt caaagtggat taggaacaag ttttatggta
    13501 gagatgaaca tcaaacaagc acttgcagat cttggagcag atgtggacga gtttgaagta
    13561 agtcatacgg atgttggaag tgtatctagc gatatggctg attatttctt cattgagcat
    13621 tctttgaaag atacgctaac ttctatacct gatgaaaagc tcgtaccttt gcaatcgatc
    13681 attgacagcg aagaagtaaa agaaaaagta acgaagatct tgtcaaaaga ataaaaatga
    13741 ttcaagggag gaactatcaa tggataaatt gttgaatttg tttgtgtcaa tcgtcagtca
    13801 gccctccatc ctcgtttctt tgattgcact gcttggtctc gttcttcaga agaagaaatt
    13861 ttcagaagtg atccaaggaa ctatcaaaac atttgtcgga ttcctagtac tgatcggcgg
    13921 tgctggttta atcagtaatt cactaacccc ttttgcttcg atgttccaaa cagctctgca
    13981 tactcaagga gtagttccta gcaatgaagc agtagtggcg attgctttgc aacaatacgg
    14041 tacacctact gctttgatca tgctagtagg gatgatcgtg aatattttac ttgctagatt
    14101 cacaagatat aaattcattt ttcttacagg tcaagcaatg ctttatgttt cttgtctaac
    14161 agcagtcatt ttagtaagcg cggggtttac tgcaaatttg tggatgatct tattaggcgg
    14221 ggtcttcgaa ggaacactat taactattac gcctgctctt tgtcaaccgt ttatgaagaa
    14281 catcactgga aacaatcaag ttgcgttagg gcataccgga aatatcggtt atgcttctag
    14341 tggatggatc ggaaagtttt ttggaaacaa agaccgttct acagaggaca taaagattcc
    14401 taaaagtttc agctttttgc gagattctac tgtatcgatc atgctcttga tgtcgatcgt
    14461 atatgtcgca cttgctttgt tagctggtcc tggatttgtt gaaagagaac taagcaatgg
    14521 agacaacgct gtaatctttg cactgatcca agctggaaca ttcacagctg ggtttgttat
    14581 cgtccttcaa ggggttcgaa tggtgttggc agaaatcgtt ccagcattcc aagggattgc
    14641 taaaaaactc gtacccgatt ctaaaccagc attagatgtt cctatcgttt ttccatatgc
    14701 acctaatgct gtattaattg gcttttttgt tagcttcttt gtaggaacac tatccatgtt
    14761 tgctatgata ggcttgcaaa ctacagttat cattcctggg gtagtaggac atttcttctg
    14821 cggagctgct tctggtgtct tcggcaactc gactggcggt agaagagggg cggtattagg
    14881 ttctgctttc aatagcttat tgattagttg gctgccatta tttatccttc cagtattagg
    14941 agatcttaaa atcgcttctt ctacatttgc cgatactgat tatctagtac caggacttgt
    15001 acttgggaaa ctaggagaat acggtcaaat agccttgaca gttggaatca tcgctttctt
    15061 tttacttgtc gtattatcta gctttctttt tgccggaaaa agaacaaaca ttgtttctaa
    15121 agaacagaat taagcaaact tcaaaataaa aattgcttgg acaatatcag aagaagattt
    15181 aaagtcttct gatatgtcgc aagcagtttt ttgttttatg catttatttt tgaatagctt
    15241 gttaagacaa gtggctgttt tgctagaaga ggataacttt taccattcat tttcactgtt
    15301 ccgcctgttc tatcagaagt aatcacgatc ttatcttttg ttttttcaag cgaaatcttt
    15361 ccatctttga taggtagcgt acaattcagt gtttcaaatg aatctagatt aggttgtatc
    15421 ataaattctt cataacctgg ctttgttgga tacaaaccga taaaatagcg tcctaaaaga
    15481 tagattgggc ttgctcccca tgcatggcat aagctttttc cataaggatc tccatacata
    15541 ctgtactttt catttcctgt ttgagtaggg tcgtattctt cccagaaagt taccgcatca
    15601 tgagataaca tccctcccca ataatcaaga atcgtttggt agacgatatt cgtctgtccg
    15661 agtttgcata aagcatcttg ttcatagaat ttgaaatatg gagtagtaat ctgtgtgata
    15721 gtgtcattca ataagacatt ctttaagatc gattgctgtt ggtctttgtt agcaaaatcg
    15781 aataaaacag cgaaaatatt cgcatgacgc gtcacatttc ttctcccaga ttcatagcaa
    15841 tcgataaaag cgccaagttc ttcatcccaa aagtagtcaa ataggttttg tttcaatagt
    15901 tgatattttt cttgatagag atgagtatga taattttcgt tctctccata tgtttcagct
    15961 aagatatctc cgcaagtgat catggaatga taggctttca gtaataagac ttgttctgca
    16021 gcaagcgttc cttctttatc catttcagac caatcaataa atatccaatc ttttgctctc
    16081 ccataaataa accctaattc attcgtttgc tccatcaagt atctcattaa tgattgcatc
    16141 ttagggtaga cgatgcgtaa aaattcatag tctttcgtca tcatatagtg gttttcgata
    16201 ccaatcaacc acaagatcga atagtcaacg atagtattca tatgctgctt gatttcattt
    16261 tgtcctctta atgctaatat cgttcgttta ttgatttcct cgtcaaaaaa aagatattga
    16321 ttgatcatga atgattgata cgcatctcca gaccaaatcc agcgatctcg tttgataccg
    16381 tcgataaaaa acaaatcact acataacttg aaggcttctt ctgatactga ccagatctga
    16441 ttcagtaaag aagaatcgct tgaaaaagca gcttgtttcg aaagagataa aaattcatga
    16501 aaaacttgaa gttgaaccat tccagtagaa acctctggta taaacaaata gcgaaaagcg
    16561 cgtttacgag ctttcatttt tcttgtcatt cctatttgct tatagtagca ataatcgata
    16621 tccatagctt ctacatcgga ctctccataa catagagtga tagattcgat atggtcatcc
    16681 agagatatta gttctacaca gccattcact gctcgcccaa aatcgaataa gattcctccg
    16741 ccaatctccg tttcagtgac tggatggacg cattcttttt gataatagat ttgatttgga
    16801 tcttttgcga tatcgacata aagcggactc cagccagctg gatattcttc aataaaatta
    16861 cttgctgtcc agccaatatc tgattttact tcgtctccct cgataaacat cgctggtagc
    16921 cctgatgtat gtcctacaaa gatacgaatt tttgctttac cagctggaca agtcagccat
    16981 tttccacatg gatgtttttg tccattaatt tctacatacc caactccttg gatcgtcact
    17041 ttaaacatgg aaggttgatc aagaaagtaa tatctcttaa acttgacgtt ccgatggcaa
    17101 tcatccatgt accagtacgc tggccagtca aaacctcgtt cttcccgttg gaaattttgt
    17161 agcagtccat ggcggatctc aaagtctcca ggataccaaa gccatctatc tttatgtttt
    17221 tcatttgtca ttttacacga cctccatcaa atttattttg ttgtcaattg ttcttttatt
    17281 tcttcatcta ctcttatcac tgtctttcta ttcgtcctgt gtactactag aaggctaata
    17341 agcgcgctaa tcaacagcaa gatactgcca ttgacgaaaa cagcaagtgt tttactccca
    17401 agagaagcaa aagagagaaa gagcaaacca tatggtgcaa tgaaagtacc taaattagcg
    17461 ccaaccaata agattgaagt attgaaactc tccgcgtact tgggcgcttg gatacttact
    17521 tggttgaata agtaaggaat gaataacgaa aaagaaattc ctgttaggat cgcaccaaac
    17581 ccaatcacat acaggtttat cacagtggca atcaatacaa agccgattcc catcagtagt
    17641 aaagaaagtg taagcatata gcgatgtgct tttgtctgaa gccagccaaa acaaactccg
    17701 gcagccatac ttgccaattg catgattgtt aagatattcg ctgcattagt ttcgtttcct
    17761 agagaataag tcgtcaggac tgtggctaat ttaatcgata cagcattata taccacgatg
    17821 accagcaaga gaaaaatacc ataactaatc attttaggat tcgttttgat tacttgtctt
    17881 gttggggctg tgccatgcga ttgaagttca gttgtttcag gtgtaggaac aaacaaaaca
    17941 aacaagacaa gaataggtaa gctgatggcg tagactaaaa aggtatattg ccaaccgaac
    18001 tgtactagct gtcctgcaac aaatgtcatt aaagctgcac caaatccttg aaatgcactt
    18061 tgaaaaccaa tcatagaggc tcgttcatct ccgtgataaa aggcagcaat catgctgact
    18121 gccaaagaat tgatcaatcc aaagccaata cctagtccag ctcttgaagc taagacaata
    18181 ctgtaacttt gtgagaagag ggggaggata ccacaaatcg atgcaatcac taatccgatg
    18241 atcacagttt gtttttgtcc gattttccta gcaataaaac tactaatcaa aacaaaaata
    18301 gtgaccataa aagagggagc agtcgttaat aattcaacag cagataaaga acgcgtggca
    18361 taagtagctg acatactggg aattgcagac gtgactgcac cagcagaagt caaaataaga
    18421 gagactgaca gcagagaaaa tttcatcatc tttttattca taagcatgtt cctttcattt
    18481 ttgtaagcgc ttatcaaaaa tatagtatga agtggttatt ctctcaatag cggaatcaca
    18541 ggaagttcat gtcccaacca tagaaagtct ttatgaaacc aaaaatagct ttgcatataa
    18601 tagcagtaca gcgtgaacta ataagcgaaa gaaaccgctt acaaaaagga gggagtcaat
    18661 tggaactagc tgaattagac attttttttg cgtaacgaag aatcaatcga aactctgcag
    18721 cgtgaacaag gaatgaatat caatgattta aacttagctt accatcttca gacaccgcca
    18781 aaaatccacc gaggggatct atttcaacag ggaaatattc atatcagtaa acaccgtcga
    18841 tatgcggaag ttcccgagca tacacatgat tttgttgaaa tcaattatat gtatagcggt
    18901 acatgtttac aaattattaa tggagaaagg atagagatgg aagaaagtga cgtggtgatc
    18961 attgataaag aagctactca aagaatcgaa tacacagggc aagaggatat tttaatcaac
    19021 atcttattaa aagaagaaac cttatctaca gagatattga atcatttggc aagcacaaat
    19081 aatctacttt cttctttttt gataaatgca tcaaaagaaa aaggggaaca tacacaattc
    19141 attcattttc gcggcggaaa aacaagtcgt ttgacgcatt tgattgaaaa tatgctgttg
    19201 atttattttg atacatacag tcataagatc cgttcgttaa atctctatac atcactatta
    19261 ctgatcgaat tgactcattt attagaacaa caaagctttt catcagcaga tggatgcgca
    19321 gaaaatgaga tgcttagagt attagattat atcgataagc attttacttc ttgtacgtta
    19381 aaagaaacag cagctcattt tggctacaat ccggtctatc ttagtaataa attgaaaatg
    19441 tattttgatg cttcctttca agaactggtt ctaaagaaaa aattcgactt tgcgttggaa
    19501 ctgattcaag aaacgagttt ttcgttggaa gaaatcgcta atcgtatcgg atataaacaa
    19561 acgacttctt tattcaaatt attcaagaag tattcaacat gtactcccaa tgaatggaga
    19621 aataaacatt aagaagaaaa tctttcacta aagcatatga aggaaaattg ttttaggtgc
    19681 gattgctgtc ttattatatc gttgcagcga atttttttgg atttattttc agttctaaac
    19741 ttttggttgt attatcaggg gatataggtg taaacatttg tcatttatct gttatgcgta
    19801 ttcaggaatt cgtttccatc aaaaattata ataacaacta ttataattaa catgttgata
    19861 aaatgaataa acaagactat acttttcatg aaagtaacaa atagttatga aaaggagctg
    19921 tttattcatg aaaaaaacaa gtcgtaaagt agtaatcgtc ggtacaggtt ttgttggaac
    19981 aagtatcgct tatgcaatga tcaaccaagg aatctctaat gaattagtat tgatcgatgt
    20041 gaatcaagaa aaagcagaag gggaagcatt ggacctttta gatggtatgg cttggggcga
    20101 tgaaaatgtg gctgtatggt caggtggcta tgaagaatgt aaagatgctg atatcgttgt
    20161 tatcacagct ggtatcaacc aaaaacctgg tcaatctcgt ttagatttag ttaaaacaaa
    20221 tgcgtcaatc atgcgccaaa tcgtaaaaga aatcatggga tcaggattcg acggtattat
    20281 cgttgttgct tcaaacccag tagatatttt gacatatatc gcttggaacg aatctggtct
    20341 gccaacatca cgtgttatcg gtactggaac aacattggat acaactcgtt tccgtaaaga
    20401 aatcgcattg aaattaaaag ttgacccacg cagcgtccac ggctatatct taggtgaaca
    20461 tggtgattca gaagttgctg catggtcaca tacaactgtc ggtggtaaac cagtgtttga
    20521 aattgtagaa aaagaccatc gtattgccca agatgaatta gatgtgattg ctgataaagt
    20581 ccgtaatgct gcttatgaga tcattgatcg taaaaaagca acttactatg gtattggtat
    20641 gagtactgca cgtatcgtaa aagctatctt gaataacgaa caagctgtat tgcctgtatc
    20701 tgcttattta actggtgaat acgacgaaaa agatatcttt acaggtgtgc catcaatcgt
    20761 tgacgaaaat ggtgttcgtg aagttgttga attatcaatc aacgaagaag aaaaagcaat
    20821 gttcagcaaa tcaacaagtg ctttaagaga agtattgaac actgtattat aagaaacaat
    20881 gaatatgaac taaaaatcct aaaactgctt aacagctgaa atagctgtta agcagttttt
    20941 tattgatatc taataggtaa gcaaataaat agaatggtta aaattaatcg gtccgaaaat
    21001 gtatccgtac atatgaacat atgaaattta atctgttgat tacaataagc gaatcgttat
    21061 caatgtttat ctgtggttaa ttaaaaaatc cttccgcaac ggcaaataca atgattcata
    21121 tctaattcgt tcatatttga ttaactgaat aaagaaactg gtttttatca ttcaattaaa
    21181 cgacaaaaaa agtaagaaaa actgcaaaat ttttttataa ttttgcataa gcagattttg
    21241 atggaaaaat gacgattttt gataaaaaac cgttcgagag aggtttatca tt
//
LOCUS       DADVXO010000042        20530 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.043, whole genome shotgun sequence.
ACCESSION   DADVXO010000042 DADVXO010000000
VERSION     DADVXO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.043"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="KB950_001295"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="KB950_001295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBB6857522.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="KB950_001296"
     CDS             1011..2258
                     /locus_tag="KB950_001296"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBB6857523.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="KB950_001297"
     CDS             2334..2678
                     /locus_tag="KB950_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857524.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="KB950_001298"
     CDS             complement(2795..3121)
                     /locus_tag="KB950_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857525.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="KB950_001299"
     CDS             complement(3736..4938)
                     /locus_tag="KB950_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6857526.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="KB950_001300"
     CDS             5055..5570
                     /locus_tag="KB950_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6857527.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="KB950_001301"
     CDS             complement(5769..6215)
                     /locus_tag="KB950_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBB6857528.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="KB950_001302"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="KB950_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBB6857529.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="KB950_001303"
     CDS             complement(8094..8723)
                     /locus_tag="KB950_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6857530.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="KB950_001304"
     CDS             complement(8707..9171)
                     /locus_tag="KB950_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6857531.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="KB950_001305"
     CDS             complement(9658..10926)
                     /locus_tag="KB950_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6857532.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="KB950_001306"
     CDS             complement(11157..11894)
                     /locus_tag="KB950_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6857533.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="KB950_001307"
     CDS             complement(11961..12530)
                     /locus_tag="KB950_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBB6857534.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="KB950_001308"
     CDS             12658..13530
                     /locus_tag="KB950_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6857535.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="KB950_001309"
     CDS             13872..14744
                     /locus_tag="KB950_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6857536.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /locus_tag="KB950_001310"
     CDS             14741..16699
                     /locus_tag="KB950_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6857537.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="KB950_001311"
     CDS             complement(17031..17882)
                     /locus_tag="KB950_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6857538.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="KB950_001312"
     CDS             18005..18472
                     /locus_tag="KB950_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBB6857539.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="KB950_001313"
     CDS             complement(18735..19217)
                     /locus_tag="KB950_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBB6857540.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="KB950_001314"
     CDS             complement(19317..20180)
                     /locus_tag="KB950_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB6857541.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6174 a         4220 c         3720 g         6416 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa
//
LOCUS       DADVXO010000043        20367 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.044, whole genome shotgun sequence.
ACCESSION   DADVXO010000043 DADVXO010000000
VERSION     DADVXO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.044"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(58..1161)
                     /locus_tag="KB950_001315"
     CDS             complement(58..1161)
                     /locus_tag="KB950_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBB6857542.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1383..1853)
                     /locus_tag="KB950_001316"
     CDS             complement(1383..1853)
                     /locus_tag="KB950_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBB6857543.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2186..3826
                     /locus_tag="KB950_001317"
     CDS             2186..3826
                     /locus_tag="KB950_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBB6857544.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3980..5302
                     /locus_tag="KB950_001318"
     CDS             3980..5302
                     /locus_tag="KB950_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBB6857545.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5364..5795)
                     /locus_tag="KB950_001319"
     CDS             complement(5364..5795)
                     /locus_tag="KB950_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857546.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5859..6695)
                     /locus_tag="KB950_001320"
     CDS             complement(5859..6695)
                     /locus_tag="KB950_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBB6857547.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7437..8063)
                     /locus_tag="KB950_001321"
     CDS             complement(7437..8063)
                     /locus_tag="KB950_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857548.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8358..8693)
                     /locus_tag="KB950_001322"
     CDS             complement(8358..8693)
                     /locus_tag="KB950_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6857549.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(8680..8964)
                     /locus_tag="KB950_001323"
     CDS             complement(8680..8964)
                     /locus_tag="KB950_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6857550.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9020..10543)
                     /locus_tag="KB950_001324"
     CDS             complement(9020..10543)
                     /locus_tag="KB950_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6857551.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10536..11711)
                     /locus_tag="KB950_001325"
     CDS             complement(10536..11711)
                     /locus_tag="KB950_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6857552.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(11715..11900)
                     /locus_tag="KB950_001326"
     CDS             complement(11715..11900)
                     /locus_tag="KB950_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857553.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(11866..13560)
                     /locus_tag="KB950_001327"
     CDS             complement(11866..13560)
                     /locus_tag="KB950_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6857554.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13557..14030)
                     /locus_tag="KB950_001328"
     CDS             complement(13557..14030)
                     /locus_tag="KB950_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB6857555.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14099..14254)
                     /locus_tag="KB950_001329"
     CDS             complement(14099..14254)
                     /locus_tag="KB950_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBB6857556.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14388..14768)
                     /locus_tag="KB950_001330"
     CDS             complement(14388..14768)
                     /locus_tag="KB950_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6857557.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(14772..14987)
                     /locus_tag="KB950_001331"
     CDS             complement(14772..14987)
                     /locus_tag="KB950_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857558.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(14990..15397)
                     /locus_tag="KB950_001332"
     CDS             complement(14990..15397)
                     /locus_tag="KB950_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBB6857559.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(15663..17138)
                     /locus_tag="KB950_001333"
     CDS             complement(15663..17138)
                     /locus_tag="KB950_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBB6857560.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17128..17976)
                     /locus_tag="KB950_001334"
     CDS             complement(17128..17976)
                     /locus_tag="KB950_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBB6857561.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18013..18375)
                     /locus_tag="KB950_001335"
     CDS             complement(18013..18375)
                     /locus_tag="KB950_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857562.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18376..18534)
                     /locus_tag="KB950_001336"
     CDS             complement(18376..18534)
                     /locus_tag="KB950_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857563.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(18675..19299)
                     /locus_tag="KB950_001337"
                     /pseudo
     CDS             complement(18675..19299)
                     /locus_tag="KB950_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19296..19607)
                     /locus_tag="KB950_001338"
     CDS             complement(19296..19607)
                     /locus_tag="KB950_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857564.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(19651..19944)
                     /locus_tag="KB950_001339"
     CDS             complement(19651..19944)
                     /locus_tag="KB950_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857565.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20138..>20367
                     /locus_tag="KB950_001340"
     CDS             20138..>20367
                     /locus_tag="KB950_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6857566.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTT"
BASE COUNT         5782 a         4236 c         3386 g         6963 t
ORIGIN      
        1 taaaaatagg agtacggcat catctgccgc actcctattt ttttcgtcat ttgtttctta
       61 aataaaacga ttctcacgca attttatttt tttgatatct tctactgttt gtgttccagc
      121 taattgcatg accatttcca attctgtttt aaagaaatca aacacttgat ggactcctgt
      181 acttcctcct aaagacaatc cataaataac gggtctgcca atagcaacaa gatctgcacc
      241 tgatgcgatt gctttgaaaa catgctgtcc gcgtcgtaca ccgctgtcaa acacgattgg
      301 tacacgttta tctactgctt ctgccacata ttgcaacgaa tcaaacgctg ctggaccacc
      361 atctagttga cggccaccat gattagatac ccagattccg ccagcaccag attctaatga
      421 acgatagaca tcttcttctg actggacacc tttcacatag actggcaaat cagaatgagc
      481 agcaataaat tctacatctt ttggactaag cttctgtttg gaagatttgt acacagcatc
      541 catcgtttgg cctactcctg attgataagc ctgaacgatc ggcattggca gtgggaaggt
      601 aaacccattt ctgcggtctg tttcacggtt tccgccaact gttgcatccg ctgtcaatac
      661 gattgctttt gctccattac gcttggccac ttcaagaata tccaaattga tcccatcatc
      721 tttgctcata taaaattgaa accattgcgg tgctttctcc ccgcctgctt ctcttatctc
      781 ttctaatgta caagaagcgt aagaacttgc tgtatagatc gtcccaaaat ctgctacacc
      841 ttttgcagaa gctttctccg ccttcacatg agctaagcca tgagcagcaa ctggtgccat
      901 gatgattggt gcagtcagcg tttctttgtc aaaatgtgta gttgtatcgg gtaattctac
      961 atcttttaat acatgaggaa taatcagctg atgattgaat gcccgttcat tttcttgata
     1021 agtaaataag tcgccagcac cactgctgat gtaaccataa ccaccagctg gaatcacttg
     1081 tgaagcggct atttctaaat cttccatatt gataaaatcc actattcctt catttgtacc
     1141 tgcttgatac gttttttcca tttcaatgcc ttccctctat aaaaaattga atgaataata
     1201 tgatagcgtt tacacacttt atattactct tccgtattga tacaggcaaa taatctgttt
     1261 acatttcagt cattccagtt gttactactc tgaaagaaat gtttagaaca caaatagata
     1321 aaaaacgatg ggactttgac aaaagtcgtg tcaaagtccc ttttacgaaa tactgtggat
     1381 gcctattgaa ataagtgcgg gttgtttctt tgtatttcaa tcgtttcttg ccattcttcg
     1441 tttaattttg gattaccgtt ttcttccttg aacgcataag gtcccaatcc aaaaagctca
     1501 gttagtattt gattcacgat tccatatagc tcggtgtgat cggacacatg attattgaag
     1561 atcatctcat tatcaccacg tttgaatctc acattcgggc tctcatgatg aacctctatg
     1621 cagttttccc ctttttttaa ctgacggatt tctgaaaaca tctcataaat atcttctcca
     1681 ataaaacctt cagcaagcgc cagactgtcc acaagaccgc ctactgtcaa ctcagagatt
     1741 atcgctttgt tggcaccaag ctttaatgta tagcttgttg cttctactac gccgcatgca
     1801 ttgcaaccac cttctgatag ttcgcttttt ctgatcaatt gaacttcttt cattttctgt
     1861 tcctcctctt agttttctac ttatgtattt tttatcacct gacgtaagtg tattttatcg
     1921 tcacgatcac gtgatttatt cttcagagat tctatttatc tatctaacat atcctttatt
     1981 cttctacttt tctttcttta gcatataaga gatacgcata gtaatcaagc aaggattgtt
     2041 gatataaaaa gaacaaatac cgctattttg atttcttttg actctattac tcatataaat
     2101 aaaatttatt atacctgctc tttttgacta tatcctcatc tgactcctat acttcgtttg
     2161 tattaaaact taggaggaat cattcatgct tacagggcta aacttattaa ataatccttt
     2221 tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatacggaa tcactggtat
     2281 gcttccaagt actgtccaaa cattggaaca acaatctgtc caagcttacg gtcaatactt
     2341 aagcaaacag accgatttgg aaaaacgtat cttcttaatg aatctattca atacgaaccg
     2401 gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg tcgtttatga
     2461 tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac cacaagatgc
     2521 tgcatttctt tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa acgcagcaga
     2581 tggacgcgac attcgtttga tcgttgtgac agatgcagaa ggaatcttag gtatgggtga
     2641 ctggggtgtg aacggtgttg acatcgctat cggtaaattg atggtctaca ctgctgcggc
     2701 tggtatcaat cctgcacaag tcttgcctgt atcaatcgac gctggtacta ataacgaaga
     2761 attattgaat aatccattat acttagggaa tcgtcatgct cgtgtagaag gcgaaactta
     2821 ctacgaattc attgatcaat tcgttcaatc agctactgaa ttattcccag aattgttatt
     2881 acattgggaa gattttggac gaggaaatgc tgcaacgatc ttagaaaaat acgaagataa
     2941 aatcactact ttcaatgatg atatccaagg cacaggaatc gttgtactag ctggtgtatt
     3001 aggtggattg aatatatcag gtgagcaatt gaaagatcaa acgatcttga catttggtgc
     3061 tggtactgct ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc gtcaaggtgt
     3121 acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacagggtt tattgtttga
     3181 agatacaaaa ggattgactc ctggtcaaat cccattcgct cgtaaacgct cagaattcac
     3241 taatgctgat gaattgacaa acttagaagc agttgtaaaa gcaatccatc caacgatcat
     3301 gatcggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag aaatggcggc
     3361 tcatacccca cgtccaatca tcatgccttt gtctaatcca acaaaattgg cagaagcaaa
     3421 agcaaaagat ttgattgaat ggacggacgg aaaagcttta gttggaacag gtatccctgc
     3481 tgctgatgtg gaatataacg gtgtaactta ccaaatcggt caagcaaata atgcgttgat
     3541 gtacccaggt ttagggcttg gtttgattgc ttcaacagct acacgagtaa atgctgaaat
     3601 catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac caggagcagc
     3661 tatcttgcct ccagtagcaa aaatcactga attctctcaa acgattgctg aaactgtcgc
     3721 aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc acggatatca aagaagctgt
     3781 tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatt gttatagtaa
     3841 aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc ggcaaaacag
     3901 cagccagcca actactgctc ttttgccgct tctctttggc ttttttcata cgatacagaa
     3961 taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag ttgaggaaaa
     4021 gaaaaaagaa gggttttggt caacaaaaat taccggcgta agtttgcccc tttatttgat
     4081 catggtcctt gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga atatgattgg
     4141 cccaatcgct gttttggtca ttttcggtaa tttatttcat tttatcggaa caaaaatccc
     4201 tatcgtcaaa agttatttag gtggtggctc cgtttttgcg atctttgcct ctgccgcgat
     4261 tgctacattt gggatcttac ctgagtatgt agtaaaatcc acagaaaact ttgtcagcaa
     4321 tatgggcttt ttagactttt atattgctgc cttgattact ggtagcatcc ttggtatgaa
     4381 ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta tttcaatggt
     4441 cgtctccttt tttgccgtag gactcgtagg tatgcttatc ggaaatggtt ttgcaaattc
     4501 cgttttatat atctcattgc ccgcaatggc tggtggtgtt ggagccggag ctgtaccttt
     4561 atcaacgatt tatgcaaatg ttttaggtac ggacgcttct tctatcattt cccgcctgat
     4621 tcctgcgaca gcaatgacaa atgtcctagc tatcattggt gcagcgttag ttgctcgtgc
     4681 gggacaatca atgccaaaat tcaacggtaa tggtaaatta atggaaaaag gcgaccttgg
     4741 cgatggaaaa cctagaaatg taaaaccttc cattccacaa ttaggtgtcg gtttgatggt
     4801 atcattgact ttcttcatct taggacaaat ctgtaactat tttgttccta aagtccatgc
     4861 ttacgcattc atgatcatta tcgttgttat ctgtaaaatc gcaaacctat taccagaata
     4921 ttacgaagat gcagctatca tgttcaacaa cttgattgtc aaaaacttga cagcagctgt
     4981 tcttgccggt atcggtattg cgttattgaa tctaaatgta ttggcttctg ctttgacatg
     5041 gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt caggatttgt
     5101 cggtcgtttg tttggtttat atccaatcga atcaactatt actgctggct tatgcaacaa
     5161 ctcgatgggt ggtacaggaa atgtagctgt cttatctgca gctaatcgga tggaacttat
     5221 tgcttttgct caaatgggca atcggcttgg tggtgccatc gttttgatca tctcaggttt
     5281 cttgatgcaa ttactttctt agtcaaaaaa aatacctgat catcaagtat cggcttgatg
     5341 accaggtatt ttttgataaa aatttacagg attttcaaaa atcttttttg cattttatcg
     5401 gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc tgcagaatta
     5461 agtcttattg ctgatattcc tcgtcttttc gcttcttcta cgccagtcat caaggccttt
     5521 ccgattcctc ttttttcagc atgtttagac acagcaagtg ccataatatt gaacatcgga
     5581 tcgctgaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt tttagaagta
     5641 ggatcttcat agaccaagaa aaaatttttt tctggctcct tcaacagttt cttcatttgt
     5701 ctcaccgtaa cggttaatgg tacatcatat cctaattgtt ccgcattgat ttctcgtagt
     5761 gcttctgcat ctcctaaacg catttcccgc aacatccaaa tcaccagcca atttttattt
     5821 ttttaaatga atttttttat cacagtatac tccttttttc atttagaaca atatgtttca
     5881 gtgattttga cttgcgttag tctagattct cctaatgttt gtaagatgac gaaagcattg
     5941 taaagattga tcaaaagaac cacgaggaca attacttgaa aaataaaggt cacttttttt
     6001 ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc agcggggata
     6061 acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc aatcgttacc
     6121 aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc ttcttttatt
     6181 ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc aatacctaaa
     6241 aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca tgacgtcaaa
     6301 tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt caataccgtc
     6361 aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg
     6421 aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca accgacaatc
     6481 tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg
     6541 aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc gataagatca
     6601 agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc
     6661 agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc tcttgtttat
     6721 atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca aacgaggctt
     6781 cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaagaggt tgtgaaaaag
     6841 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gctcctcata attttcgttt
     6901 ggttgaactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag
     6961 cctgccgttc agctccgagt cacaaggaac aatttcaaaa aacagcttct catgtttgtt
     7021 gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttaagg aagagactgt
     7081 gaagtcaacg ttttgacctg attattagag aaacaaatgg caaaatagtt cttcatattt
     7141 cgccgttttt cgagcgaatg ccgaggggca gctgattgaa catcgtttat ttggaaaaag
     7201 ggattgtgac aagacgtttg tcacaatccc tttttttata tcttattaat tcattctttt
     7261 gttctattct tcttcttccg ccatgaatga gaaacccata tcaaagaact ataaaggtta
     7321 tcaaaacagc gtttatgact aaatgaatta gagttatcta tcactatttt tcaagcgatt
     7381 taaaccaaat ggcatacaat atggcataca aaaacttcaa taagccgtgg gggtttttaa
     7441 atttcttctt gttctttaaa atcaatgagt ggagctacat aatttgaatc cactttttca
     7501 aacgtcactt tttgcatcga cgaatgtttt ctttcccaag ataacgtaaa tagatttgac
     7561 tctggactta taaagtctac ttcaccttct agtattactt tcttatgcaa atattcctct
     7621 aataaatata gacttgctaa agcatacaac acattttcca aatttgcttc cttataattt
     7681 gaagaacgac catgttttac tctgttatat attttccacc aatctggtgt atcttcaggc
     7741 cacccatctc caaaaggttt tagtgaagtc aatgttaaag aattcataac cctaacacca
     7801 ttatccaaac acattaattc gctagattta ataacagctt taaaatcagt tatgctttta
     7861 ttgctactgc tatcaaatcc gcaaagttct ttgaaaataa tctcacactc cgaaccaatc
     7921 gctagtagta gtttttgata ttcaatagag aatgttttga aattattctc atcgagcatc
     7981 acaaaatctg tggtattaat aaactcattt tctaaatgta gataatactt ccaataaaaa
     8041 tttgtaaact ctgctttgtc cattaaatca cctcttttga attataacaa aaaaaacacg
     8101 tccacaacag acgtgttctt tgatttctac acaatcagct tgccaaaacc atccaatcag
     8161 tcctagcatg cctgtattaa aattgaacgt tcatcatcta tcatctttct tagtccagca
     8221 tgtttgcaac cacacctaca attttcagtc caacaataga tttcgactag ctaagtcttg
     8281 caaaattcag gcagctaacc cttcatttac taccttaatt atactaaata cccacaacgt
     8341 ttacaagtgt aatcattcta agaaatctgt ttagctatga tcgttgtata atctttaaac
     8401 gccgttcctt cattgattga aataatttta taactcttgt ttttccaagc tactttcata
     8461 tcgtttgtta gttcataatc ttgatcgtat cgaataataa acgtcaacgt tccttcaaga
     8521 atggtaccta cactagcaag cacgtctttt agcatttgtg aacgcacatt agcccaacat
     8581 tcaaatacag tttcttcact atccaccatc tgcccatctt catcttttac ccgttttgtc
     8641 tttttaaact tgactctctc agtcaattca tttacattct taatgattgg cataataagc
     8701 acctctcatt tgttgtacta atgcttgtac cgttactggt atccgttcgc tgctggcttc
     8761 ttgacggttc aaataccaat gttgagtaag taatgacacc gccaaatcaa attgctgata
     8821 attatccata tctttatccg ttgcgctact atcaatagca ctttttatat attcgcctgc
     8881 tgtatcaatc aattgttgaa taaagtcatc atcaatagtg tgatcgatcc gcatactttt
     8941 ttttatgcta tccaaaatta ccattttatc tctcctaatg aaaaaaaggc agtgggataa
     9001 accgccgccc aatatttttc tatacttttg gtgttacaac tggcgtaaac tcaatataca
     9061 ctgaagcatt ttgatcaatt ttcttataat cgttgcgaac gatcactgca agtccttgtg
     9121 agtagtaatc gaatttttcc cattgagtcg ttacttgatt tctacgagca acaaagatag
     9181 attgtgcaat atcccccata atcataggga atgttccagc tttaggattg gcaaacaatg
     9241 tatctgcaat caatactact ggcataccga ataatgattt accactaggt gctgtcacat
     9301 cgggttgtaa aatgtaacgt ccatctgaat ctttcaatgt atctaggtgg taatatccgc
     9361 tttggtttag aattaccatt ttatttaatg ctgggtctaa tgccacattg tatagttgtt
     9421 tcaaatcgtc caacgtagca gccgttttct tagtgaatgt ttttaataaa tccatgatat
     9481 gcttattgtc agtgttttct accaatttcg ctaactgatc tttgacctct tgtacaatat
     9541 tcactgctga atcttccaca acctcatttg ataaggcaat cttaccagca cgagtttcta
     9601 ctttataatc aactgaagta aacatttcag cgtcaatatc accaatttca gcaagttcag
     9661 cttttgttgc taacactgct tgttggttag ttgctactgg atatttacct tgtccatttg
     9721 atactgtttt tactgttgca tattgagcta agttataatt tgaacgtttc aaatcaaaga
     9781 cttcaccgat tacttcttcg ggaacaactg ccgctgcatt tacagtagtg actccatcac
     9841 gcacttcgcc ttgtgaacgg atatattctt catacccacg caattctgtt ttttcgttat
     9901 cgattaaagt tttttccatg ttattactct ccttttgtgc tttagaagtg gattctaagc
     9961 tattgtttgt ttgttttgct tggttgttac tcataaacga ttcgtaagaa cgcttattta
    10021 cttggacatt tgatgaatca taagcgggaa cggtcacgac actaatttca ttaagtgctt
    10081 tgattttatc tattgatcgt tcaattgtgc catcttcttt tttgtcgaat gaatctaatc
    10141 caagcacgaa cccaaaggac atagaatcaa caaccccttt tgagatattc tcatacacat
    10201 catttgaata ggtcgtgtcg gttagctctg cttcaaaatg taatcctgta tcatcgacat
    10261 tcaatttcaa cgtacctgct ttgacactag ctagtggctt tgaataatcg tgtccatata
    10321 ataagaacac atttgataaa tccacctctt ttaatgcttc gggtgtaatc acttctacaa
    10381 agccgcctaa atcttttgac ggttgcccga atttcaacgc atagcctgaa atagttttgc
    10441 cgtctgttgg ttgtttttca ttttctttct ctactttggg agaatcggct gttagctcag
    10501 cttcttctgt cagccgtttt tccttttctt cttcattatt caactggtgt aactccttcc
    10561 tgttctttta attgatattt ctccaacgta tctaaatacg taaagttcaa acttgctagt
    10621 aaacgatcgc caccatccat ggggggtagc cccattttcg accgcccctc attaatcgtg
    10681 agaagtgacc ctttgacctg atccaataca ttttttactt ttgtttcagg atcagtctct
    10741 agtaactgat cggaattaaa gcggatattg gtttcaagtt tcgtatctaa ttcactcaca
    10801 aacacattaa agtaatgaat cagcgtactt tggatatact ggagattact ttgaacggtg
    10861 ctagaatgtt cattttcaac gcctaaacgc tcaatcggta caccaaacgc cttggcaatt
    10921 tgttttgtgt tccaatcatt ggaattgata agcttcaaaa catcggtgtt tatttctaat
    10981 gttttgtagt ccattgtttc atcaagaatg atcgttctaa gagcattatc tccactactt
    11041 gaaccgttgg cttcttcaaa cttttcacga attgcgttct ttgctgatcc gtctaaatct
    11101 gacttatgaa ccttcaaaat acctgaccca ctgacaccac gagtaaagaa attgtgcaat
    11161 gtgcggttgc cagcttgttg aattttcagt tcatcacgta acgcataaag tggtggtaat
    11221 cctgttaaac catcttgggt gaaatatttg aagtgcaaaa tatcgctaga cttcacacgt
    11281 ctttttttat caccaatctg ataagacaac gtaccattat ctaattgagt aaccgttaca
    11341 cttgagttag gtaataagtg aatttcttct accttttcac cgttgcgttt aatctctgca
    11401 aagctattac cgtttagcaa catattgacc gccaaagcaa actttaaatg ccaaccatcc
    11461 atttctgaat taggtttctc gtttagtaac ttcaccagtt cattatcagc ttgtggcata
    11521 ttgtttttga ccaattgaat cggacttgaa gcaatatcac tagcaataat tcgaacggct
    11581 gcaaacacat cactatttct taatgcacga acgcttgtaa agctggtggt gtaatcatcc
    11641 gattggattg ataccacatg ttgtaggaaa ggatctcccg ttgtattttg tctcgcttta
    11701 aaaaatgcca tcttttaacc tcccttctgt tctgtattta gcatgaccgc taccccaata
    11761 agcactatgc ccagcgctaa caagccatag tagacatttg ttagtaaggt gatagcaacc
    11821 aacacacaca ccaatccaag aagtaaaaga acggtatgaa tgtaattaga aagaaaattc
    11881 ttcacttgta taaaattcat tgtctgcttt cgctccttcc gttgtggtga agtaatccat
    11941 tgcaaacaca taggcattga ttaatgcagc tattggatca attttgttac tgttctttga
    12001 tttattaatc tgaatgccgt tgttatccgt ttttaaaata gcgttgttca ctgcatgggt
    12061 gagaatcgta tttttattgt gaataacgtt gccttcataa acttgttctc taaaagttcg
    12121 ggtcggaacg tttagagtaa tcgttccttg tcttacttct aacattgggt agttggcttt
    12181 ttcagcttta ctgataagcg aattagcgtt atacgggtca taacaaatcc ccatacattc
    12241 caaatcattt tcttgaatca aatcttgaat aaactgaaat acttggtcat agtccacgat
    12301 tccactttct aattgagtaa tggaacactc accagctttt tctagttctc gataaggcaa
    12361 accgtcgcgc ttttctttgt cttgaagccc atatttggtg gctacaaagc tatgtgaatc
    12421 acaataaagt tcgccgttat ctagcggtac gatccatgaa acgctagtta aatcatctgt
    12481 tttagataaa tccactccga tataaacggg cttgcctgta atatcttgtg gttctacttc
    12541 ggttgcctgc cagtcttcag caatcatata gctgtcctca ctcgcttgtc tccataaatt
    12601 gaagtttttc accaatacag aattaaggtt attttgtttc aaagcaaggt tcacatcatc
    12661 ttgaatggtt ggaatcatta cttttttgat ttcttcactt tcaaaaatcg gattggcttt
    12721 gatccatttc tcttgatcgt gaatttcttc gggatcgtcc aattcccata tcgctataaa
    12781 gtacctgtcg gcttgttctt cgccttttag cacacgatct aacaataagt attcctcgta
    12841 cataggaacg tttaaatcaa gcccagcggt actaataaca cacaataagc cgttcttttg
    12901 ttgggtcatt cctgacttaa tgacgttgta cgttttacga gtttttgctt cgtgccactc
    12961 gtcaagaatt gcggttgttg gtgcaaaccc atctagcgtg ctggtttcac tagccagtgc
    13021 catagcgaac gaattagagg gcaagtgctg aatcttagag ttcataattt tcagttgtgg
    13081 tcttaaaaac ttactagact tgaccacgtt tctaagtgaa ttagacagca tatcatagcc
    13141 caatttagct tgtttcaaag cgttacttac aaacaatact tgccttgctt ctgctggttc
    13201 ttgttccata atcaacgcat tggcagccat gccacttgcg atatacgttt tcccattctt
    13261 acggctcata ctggtaaacg ctcgattgaa tcgcctgtaa ttcccttctt tagtacgcca
    13321 gccataaaga ctgcctagaa tgaatttttg aaatcctaac atcttgattg ctttaccgtc
    13381 cgtagatggt agcaactcaa tgaatttaat cgctttggtg gcttgttctt catcgaacac
    13441 ataagggaag ctatctgact tagaacgctc caaatcgctt atatgacgct cacacgcttg
    13501 gataattttc ttgggtgcaa tcagcttacc tgataggact ttctcaatgt aatcattcat
    13561 gaattcatca actctttgaa cgggtcttcg ggttcatcat ttttaacctt gttcaaagct
    13621 agttttgatc gtgattctaa ggtcatacct agtgaggtgg cttgtttttt caaatcactc
    13681 atagcttgtg attgaacccg aaccgctgga ttggctttct ttactccgtt ttcgcatgtg
    13741 ataaacgtac ctgattttct tatctcggct tcggctgttt tcattcgagc aaaggctaaa
    13801 caataggcta ccaatgaacc ataatctgct tcactcaatg gataatcttt ttttaatgtt
    13861 ggtacgatac gattccattc acttctagcg ctcgctgcca tccagtcagg gggcgtgata
    13921 tttattaatg gttgctgctt gaacaattct tctctagcgt ccacacggat tgctttttct
    13981 tcacttgata tattgccttt tgtatcgtgt aatattttag ggtgtcccat gttgtcctcc
    14041 tttcagacaa aagaaaaagg aacgtaagca attaagctca cattccttcg tttttcgatc
    14101 agaatttttc tttatctaat ttatctatat aaatgatttt tccgttctgc atttgaatgg
    14161 tcacttgacc aaaatgaggt aactcaatgg tgcgctgttt ccctgtttta tcagtaataa
    14221 caattgtatt tttcaataaa tctcctactt tcattatata ctttttcact tgtgattaca
    14281 agtgtaatcg ttatttgtaa ttacccttat gccttttcac aacggttatt tgtaccaaaa
    14341 tgagtaggca tcgattgtca gtaagtttca cactatgccc ccttaactca aagggtactc
    14401 ttttctcgtc tttgtctttc attggctgtc ttggaattat ggtgtgcatg gcataaagaa
    14461 cgaaaattag tttcatctaa ccgtctattc caatcatctt ttagttcaat tttgtgatct
    14521 acaacatcag ctttccttat caagccttct tctaggcaat cctcacatac tggatgattt
    14581 aaccgataca agtatgaagc ctttcgccat gttctacttt gatagaattg aaaatattta
    14641 ccttcgcttt gctttctttc ttcacgctta attgccgttt gctttgcttt atgcttccca
    14701 caatacttct gcctataatc aatcaatgtt ttacatccag catgattaca ttgtttttta
    14761 actgccatag attaccaacc aaagcgatct cgttcgtcag aatcatcttg ttcccatttt
    14821 gatttgtatt caatataagg cgaaactatc ttcaagcacg ttccgtcttt aaaattaact
    14881 ttagtgattt tatcttcctc aaaactaata aagtctacat cttcttgagt gtttgaatac
    14941 tcaatgtgtt tctcaccatc gaagtatcta atactttcca tatgcataat tattctacct
    15001 ccaataaatt agttatcaat tcatcatctg ttagtagcaa agccaagaac ttatcatgga
    15061 tgtttaaaaa caaattatcc tcattcgatg taagctctcg ttgtctcgct cgatcatata
    15121 ggttaagcag acaattaaat tcatccatta gctcacctgt atcatcatag ttgccactat
    15181 ctaccaatga tcccactact agcgattgac accacgctac tgactcctgc agccaatcag
    15241 gttcttcttc cattatcttt aatacttctg gatcaaatgt ggaataatct tctgttacca
    15301 atcccacact aaatgccagc gtgttcatta gaaaccgatc catttcatct gtatattgaa
    15361 agttcggtgc tttgtttgtt gaaataatat tgagcattta ctttcctcct tgattcgtaa
    15421 cacgtggtaa cacctacgta acaggtaccc gttaccactc aatcccaacg gggacaaggg
    15481 ttctagcgtt ttggtaacag gtaacgggta aaatccagta acattattcc ccagcatatt
    15541 agctaactat ttcaaaagtt ttaaggaaat aaggtgttac cccgttacca aattgctgta
    15601 tcccttgggg cagtaagggt aaactagtaa cgggctaggt gttactgacc cgttactacc
    15661 cgttactttt tgtactttcc agctcgattt ttattttcat aggcatttga cttattgtaa
    15721 aatcctcgtc gtttatttct ttttggcagt tgcttccgtt cccaaccagg aatactgcta
    15781 ataatcaagg caatcttttt agtttctgca tctgatcgac cattcaataa atcctttgct
    15841 tgtttatcaa aaacagcttc caaaatatca gcggtgagta cgctatcaac taaataaaac
    15901 tcttgttgca tagtagctcc attatagaaa tgctgtaaat ttttctcatt acatttactg
    15961 tcaggatagt tctgataata agaacgtctc gcccacgctg gagcttcttc ccatttatac
    16021 gggatttcca tttccaaata tttagtgatt gcttctttaa ttggattctc catcttggca
    16081 tcctcttgat agtcttttgc aatttctaag acttctttat cttcatcggg actaaaatag
    16141 attcgttgtc ctttatcaaa aagcaccttt gcttcagcta acacttgaag aaaatattca
    16201 tcctttgttt taaacggatt catctttggc ttattcttac aaggcaaagg ataaaaacgg
    16261 cggtttcctg tatcatcttt caaatactgc ccatcattcg acgttccaat aaatacacat
    16321 tgccttgccc aagctatgac attccgacca taaggaggtc taattttatc gatcttggca
    16381 ctgatgaagt tttttatctg ttcaatttta gttttgttca ttgaagataa ttctccaagt
    16441 tcaataatta cattgccttg aagctgcata taatcatctt tcttttcacc tagcgaatct
    16501 aatgaatcgg tgaaaaaatc atccgtgtaa agtaatgctg gaacggtact cttaccaatc
    16561 ccttgcggtc catccaaaac tggaacgatc tcaaacttta ttcctggttt atagatacgt
    16621 gcgactgatc cgactatcca acgttttgtt acttctcgtg tgtaaaggtt gtcgtctacc
    16681 cctaaaaggt caataaacag tgtttccagt ctaggctgtt gatcccattt aactttttcg
    16741 atacgttctt ttactgggtt gaaaacattc tttctagcca catttactac tgctttgtgt
    16801 attttgttgt catcaaaaac tacattgtag ttttcttcaa tatatgccgt taaggcatcc
    16861 tcaaaactgt cttcccattc tccagcggta cgattgatcc aaggagaatc ttttaatagg
    16921 tgaatgtatc cgctaaattc gttataagca attgttccag ctaaattgct atcattggtt
    16981 aaaatcaatt ctgcattcac aacactgtta cttttaatct gatcgtcttt attgcatctt
    17041 aatcgtgaca tccaggtttt caattcaatg atattgtctt cacgctcgct ggcttcggga
    17101 aagtctaaaa tactatctaa tttatgctta tgagccatga gctttccccc tcaaatcttt
    17161 tttaatcatc gatcgaataa tttgattcac ttctttatct tttagcggtg gttcattgta
    17221 cgcatttgct gtataaatca attctgctaa agtttgatta tctacaccgt gccatagtaa
    17281 ccaaccgcat aacttagtta aatgatcgtt tctactacct tcgccagcgc cttgaacaat
    17341 catatctaat ttttttgctg tccctgtttt tgctacgggt cggtattgag tctttatttc
    17401 ttgcggtttt tttagcagta ggttcactag ccaatcggga gctggtgcaa tattatttag
    17461 agatacgttt tcaataggtt catatgctct cccaacactt cttgaaataa tcatagttgg
    17521 cgctattgga actcctttag tgataatatc aatcccgctt aaacttttac ctttatcatc
    17581 acgcccgaag atatcagcaa atgcacctgt ttcttgttgg ataattacac catcaggaat
    17641 tttaaagaag aaatggattc ctttacctgg tgtgatttcc gtataagtag aaggaaatgg
    17701 atcatacatt gaagcaagtt ttttataatt ctcaacgccg tttcgtgttt cagaatgttg
    17761 atctaggtca actaccacca tttgatgatc ttcaagcatt aacccaatat ttctagttgc
    17821 atcttccgtc caccattcat taatttgatt agaatcgtta gttgcatcat tccaaccact
    17881 agagcctttt attggaattt tagttccagg agataaggga taaattgacc atcccgactt
    17941 ttgtaattcc gttgctgctt gataattatt tgccaataaa cctctcctcc tttttcaaaa
    18001 tactggtata aatcattcag acacctccat gagcttcaac tgctcaagtt cgtatttcaa
    18061 cccatcccga acaaattcag caccttttaa ttgattctga tactttaccc atgtattaaa
    18121 tgttttcacc cttgcttctt gcatatcagt aatgtttaac catgcggtta aaatctgatt
    18181 ttttagctcg ttgatttctc gacaaattat tttgtgttct gcatgttttt tgagtacata
    18241 caaaactgtt agtttttcat tgaatgaatt tccaaagtag gttttcccga tattattttc
    18301 tagttcagcg atcccttgat taatttcctc taattcaagg ctatcgagtt ggtttaatag
    18361 ttttgttttg tacatctact tatgccctcc agttcttaat tgatagtcgt actcgtcata
    18421 agcagtgaag agatataacg ctaatcctgc aaagatagct atttttgcaa ttaatggtaa
    18481 taagccgaaa attccgccga ttaaaatgct tcctataatt aagtttcgtt tcataatggt
    18541 tccctccgat tttttaatat ggtataatcg gaacaaagaa tatcgggttt tatcccaatt
    18601 aagttgcctt tatcctgtcc gccaagactg ttatggataa ggctttttct ttgttctcga
    18661 tcatgttaat gctcctaagt tgtctgcaat tcagcagaac gattataaat tttctcactc
    18721 actgaaaact gcaacccata tttttcttta acagtcatta attctactgt ctcatctaaa
    18781 ataggttcac gatcaaccaa catttcagga gtcatatctt tcttttttac cattttggaa
    18841 tgaccataaa tagttgatac agctttattg gctatcacat tcgctttaat gtagtctact
    18901 ggttttggtt cagtaatagc gtggcttaat ttagtcattg cttctttttg gtgttctttg
    18961 tctagcatgc ggaatacttg aaagccttca agtcctgtgg actggcgtag ttctttgatg
    19021 atgtcgaaaa tccagtcttg aaatgcctcg gcttcttttt tacggctttt gaatgccatg
    19081 cggtaaatat tttttttcgt tgatgatgtt tacatcttgt gttctgccca atgaatcgat
    19141 gaccttacta gtagtaaccc catctttagg caaagagtga atggctctag taacctgttt
    19201 aagacctaac gcttccgcaa catctttcgc cactgcccac cattcatcat taatatcgac
    19261 aaatcttata atgtgtccgt tccaaatttc agttttcatt caacgtcctc cagttcaagc
    19321 cctagcaagc tcgctaacaa ctcaacttgc tgatacaaaa tttcttgata ctcttcaata
    19381 gaaagtcgtt ctttaaactc tgcttgtact aaatcgttca agccacgcaa tactccgctt
    19441 accgttggtt cttgtggtag caatttttga tagtccattt gttcgtacaa acattcaaac
    19501 gtttcttcta gcgctttgcc ttccaagttt gctaattctg ccgctgtgtc ttctgtaaga
    19561 attgttgata atactttttc gattgcttta ttttgtgtcg attccattat tatttcctcc
    19621 tattaatccc tttgccccca cggcttgcgt ttatttttgg tattcttcca taaagcgatc
    19681 acattcatct tgtgaaattc ttacgactcc atcaactata atcaacttta aaccgttagg
    19741 aacatatttc tgcatcatag tattgtatga aacgttcata tacttagcag cttgtgcctt
    19801 gttcatatat tttggaaact ggtacttttt ttctaataat gaaagtaacg ttccttgagt
    19861 ttgctctact tgttgaacaa ctcgatcgtc aataatttta aaagctggat catcaagcgt
    19921 gagtttgtca atttttaatt ccatatttat cccccctcgt tagcatacat ttctatacaa
    19981 agattttaaa aaaatgcaag aggctcttga atacatttct atacctctta taaactcaat
    20041 ataccatcat accttttcat ttgcaagaat tttaacatac ttttttgaac ttttatgttt
    20101 cacacatatt taaatcgtga tatagtagag aaaatatatg aatggtggtg aaaatgtggc
    20161 tagaaaaaaa taccccgaag cagtggagac aggacaaaga attagactgg ctagaattga
    20221 aaagggactc tcaatggaac aacttggagg taaattatcc ccaccggcca gtaaaggtgc
    20281 cgttagtaat tgggaaaatg gctataatct tcccaataac accaggctta aacaactttc
    20341 aaaggtatta gatgtatcca ctaccta
//
LOCUS       DADVXO010000044        20292 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.045, whole genome shotgun sequence.
ACCESSION   DADVXO010000044 DADVXO010000000
VERSION     DADVXO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.045"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(89..1648)
                     /locus_tag="KB950_001341"
     CDS             complement(89..1648)
                     /locus_tag="KB950_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBB6857567.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1803..4037
                     /gene="pcrA"
                     /locus_tag="KB950_001342"
     CDS             1803..4037
                     /gene="pcrA"
                     /locus_tag="KB950_001342"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBB6857568.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4054..6090
                     /gene="ligA"
                     /locus_tag="KB950_001343"
     CDS             4054..6090
                     /gene="ligA"
                     /locus_tag="KB950_001343"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBB6857569.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6196..6501
                     /gene="gatC"
                     /locus_tag="KB950_001344"
     CDS             6196..6501
                     /gene="gatC"
                     /locus_tag="KB950_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBB6857570.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6501..7973
                     /gene="gatA"
                     /locus_tag="KB950_001345"
     CDS             6501..7973
                     /gene="gatA"
                     /locus_tag="KB950_001345"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBB6857571.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7970..9400
                     /gene="gatB"
                     /locus_tag="KB950_001346"
     CDS             7970..9400
                     /gene="gatB"
                     /locus_tag="KB950_001346"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBB6857572.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9410..10465
                     /locus_tag="KB950_001347"
     CDS             9410..10465
                     /locus_tag="KB950_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBB6857573.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10576..11949
                     /gene="rlmD"
                     /locus_tag="KB950_001348"
     CDS             10576..11949
                     /gene="rlmD"
                     /locus_tag="KB950_001348"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBB6857574.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11952..12080
                     /locus_tag="KB950_001349"
     CDS             11952..12080
                     /locus_tag="KB950_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBB6857575.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12520..12708
                     /locus_tag="KB950_001350"
     CDS             12520..12708
                     /locus_tag="KB950_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBB6857576.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12786..13799
                     /locus_tag="KB950_001351"
     CDS             12786..13799
                     /locus_tag="KB950_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6857577.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13866..14900)
                     /locus_tag="KB950_001352"
     CDS             complement(13866..14900)
                     /locus_tag="KB950_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6857578.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15015..17240
                     /locus_tag="KB950_001353"
     CDS             15015..17240
                     /locus_tag="KB950_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6857579.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17258..19141
                     /locus_tag="KB950_001354"
     CDS             17258..19141
                     /locus_tag="KB950_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBB6857580.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19122..20096
                     /locus_tag="KB950_001355"
     CDS             19122..20096
                     /locus_tag="KB950_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBB6857581.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6839 a         3720 c         4345 g         5388 t
ORIGIN      
        1 ttttcccagc ctcttctttt tttatagaat catatagaat caagtagtgt agatgaatag
       61 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
      121 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      181 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      241 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      301 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      361 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      421 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      481 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      541 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      601 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      661 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      721 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      781 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      841 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      901 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      961 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
     1021 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1081 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1141 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1201 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1261 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1321 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc tctacttgtg
     1381 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1441 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1501 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1561 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1621 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1681 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1741 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1801 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1861 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1921 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1981 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     2041 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2101 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2161 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2221 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2281 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2341 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2401 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2461 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2521 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2581 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2641 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2701 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2761 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2821 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2881 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2941 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     3001 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3061 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3121 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3181 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3241 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3301 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3361 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3421 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3481 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3541 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3601 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3661 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3721 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3781 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3841 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3901 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3961 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     4021 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4081 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4141 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4201 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4261 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4321 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4381 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4441 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4501 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4561 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4621 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4681 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4741 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4801 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4861 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4921 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4981 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     5041 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5101 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5161 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5221 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5281 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5341 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5401 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5461 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5521 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5581 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5641 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5701 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5761 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5821 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5881 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5941 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     6001 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6061 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6121 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6181 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6241 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6301 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6361 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6421 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6481 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6541 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6601 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6661 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6721 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6781 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6841 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6901 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6961 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     7021 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7081 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7141 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7201 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7261 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7321 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7381 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7441 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7501 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7561 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7621 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7681 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7741 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7801 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7861 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7921 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7981 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     8041 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8101 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8161 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8221 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8281 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8341 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8401 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8461 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8521 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8581 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8641 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8701 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8761 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8821 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8881 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8941 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     9001 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9061 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9121 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9181 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9241 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9301 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9361 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9421 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9481 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9541 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9601 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9661 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9721 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9781 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9841 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9901 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9961 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
    10021 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10081 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10141 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10201 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10261 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10321 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10381 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10441 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10501 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10561 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10621 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10681 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10741 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10801 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10861 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10921 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10981 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    11041 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11101 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11161 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11221 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11281 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11341 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11401 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11461 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11521 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11581 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11641 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11701 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11761 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11821 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11881 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11941 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    12001 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12061 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12121 tgtttcttag tctttttagt tagtctacat tattttggcg atttgccaat aaggactgtt
    12181 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12241 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12301 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12361 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12421 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12481 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12541 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12601 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12661 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12721 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12781 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12841 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12901 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12961 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    13021 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13081 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13141 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13201 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13261 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13321 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13381 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13441 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13501 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13561 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13621 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13681 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13741 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13801 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13861 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13921 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13981 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    14041 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14101 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14161 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14221 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14281 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14341 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14401 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14461 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14521 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14581 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14641 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14701 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14761 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14821 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14881 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14941 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    15001 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15061 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15121 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15181 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15241 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15301 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15361 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15421 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15481 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15541 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15601 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15661 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15721 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15781 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15841 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15901 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15961 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    16021 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16081 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16141 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16201 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16261 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16321 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16381 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16441 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16501 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16561 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16621 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16681 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16741 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16801 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16861 atgaactgga tttgacaaag ctgtctcctg aagaaaaaaa gcaagtaaaa gaccaaatta
    16921 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16981 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    17041 tgtttttagg gaggatacta gcttcagctc aaccagcgtt ccatgaagta tatctgatgg
    17101 ggttagatga tgaggcactt tatcaggaac agacctcgaa gcggatattt tcgggggccg
    17161 aattgatgac agttggactt tacttccccg attttcaagg tgatttccaa acagaactgc
    17221 ttcatttcaa aaagttatga gagagaagga aaaaagtatg aaagcaaata taaaatggtt
    17281 agatgatcca caagttttta gagtgaacca atcaaatgca catagcgatc atgtttgtta
    17341 tgcaacaagg gaagaagtag aaaccacatc ctcccttaaa cagtctttaa atggatcatg
    17401 gcgtttttgt tattcgccaa atccttcggt acgaccaaaa acattttata aaaaagattt
    17461 tgatgcctgt tcttttgatg aaatcactgt tcctagtcat attgaattag caaattatga
    17521 caagatccat tacatcaata cgatgtatcc gtgggaggga catgtatttc gccgtccccc
    17581 tttcacgaca agccctgaga aagaggcgga agggatcttt tcagctgcta aagacaatcc
    17641 ggttggttcg tatttgacga cttttcgatt agaagaaggt ctaaagggaa aacaaatctc
    17701 gattcatttt gctggtgtag aacaagcaat gtttatttgg ttgaatggtc attttatcgg
    17761 gtatgccgaa gattcgttca ctccgtctga atttgattta acaccttatt tggaagaaga
    17821 aaatattcta gctgtggagg tttacaaaag aagtacagct gcattcttgg aagatcagga
    17881 ctttttccga ttttttggga tcttcagaga tgtcttctta gttgctcgag gacaaaacca
    17941 tttagaagat ctttggatca aacctcgctt agatgacaaa tgtcaaaatg gcaaacttga
    18001 agtagatttg tcttttgagc aagtagccaa aaacagtcag ctgaatgtag cgatactaga
    18061 tcctgatggg aaggaaatct atcaacagca atatccgtta aaagagcaaa tgacgattcg
    18121 catcgatgga tttgaaaatg tggcactgtg ggggcatttt cagcccaatt tatataagct
    18181 tgaattggag acgatagacg aagaaaaaag agtaacggag tatattgact attccttcgg
    18241 ttttcgtaaa atcgaaatca aagataaaat catctacttc aatggaaaac ggatgattat
    18301 caacggggta aatcgacatg agtggcatcc gaaaaaagga cgcgccatca ctatagaaga
    18361 tatggaaaaa gatttggcta tcataaaaaa tgcaaagatc aacggcgtac ggacatctca
    18421 ttaccccaac cagatcactt ggtattattt atgcgaccaa gcaggaatcc atgtgatggc
    18481 ggaaacgaac ctcgaatccc atggctcatg gcaaaaaatg ggcgcatttg agccttctta
    18541 caacgtacca ggtgcagtac ctcagtggca ggcggctgtt cttgatcgag caaaaacgaa
    18601 tttcgaaaca ttaaaaaacc atccaagtat tcttttttgg tctcttggaa atgaatctta
    18661 tgcggaagaa aacattcgac aaatgaacga ttacttcaaa aaagtagacc ccgatcgact
    18721 tgtccattat gaagggaatt ttccaaatcc agcattcgaa gattcgattt cagatgtaaa
    18781 aagctgcatg tatgcgccac cagaagagat cattgactac cttgaaaata aagcggaaaa
    18841 accgttcatc ttatgtgaat atatgcatga catggggaat tcgttaggtg gaatggattc
    18901 ttacatgacg ttgcttgatc gttatgaaat gtaccaagga ggctatatat gggattttaa
    18961 agatcaggcg atccaagtga ctgatccaat cacaggcaga gaagtgatgc gctatggcgg
    19021 tgactttgac gataaaccaa gtgactatga attctcaggg aatgggatcg tttttgcaga
    19081 tggacaagaa aaacccgcca tgcaggaggt aagatattat tatgaaaaat acagtaaata
    19141 aaagtcatat ggatacggaa aaagttgcaa tcgtcttcgg cgactgtaca ttaggtgtca
    19201 aatcggggaa tacgcattat attttttctt atacaagagg cggactggaa tcgctcaata
    19261 aaaacggaaa agaatggcta taccgggaaa cacttccagt tttttggcga gcaacgaccg
    19321 ataatgacag aggtagcgga tttggattta aatccggtat gtggttagga gccggattgt
    19381 atccaaaagt caaagaaata gaagtactgg tggacaataa aaagatcgat ttgccgattg
    19441 ctccagtaaa taatcaatat tcaagtaatg aatacgctga aacggttgaa atcattttta
    19501 cactggaaat caacacagta ccaaaaaccc aagtgacaat ttcctaccaa atcgctggag
    19561 agggtaaaat gcttgtcaat atgcattatt atggacaaaa aggattaccg gaattacccg
    19621 cattgggttt aaggttcatt atgccaacga aagcaaaatt ttttgagtat gaaggcctat
    19681 caggggaaac atatccggat cggatggctg gtggtgtcga aggtatttat aaagtggcag
    19741 gactccccgt ttccccatac cttgttcccc aagaatgcgg catgcatatg gaaacgaaat
    19801 gggtagaact aacaagaaat caaacgttgg ataatacgga taaagatcaa gaagattttt
    19861 cgttgcgatt tgaacagcag gatacgccat ttgctttttc cgctttacca ttcaaagcag
    19921 aagaactgga acaggcaaca catatagaag aactaccgcc agaaagacga acggttctta
    19981 cgatttttgc aaaagtaaga ggagtcgggg gtatcaatag ctggggagcg gatgttgagt
    20041 caccttaccg aatcagcagc gaagaggacc atgaaatcgg ctttattatt cagtagcaat
    20101 ctttagatta ttatttgaaa aacggagttt acaagacaaa gctgctttat tctatatgtc
    20161 atgaacaaga gagtaaaaaa gttctcagat acttttgttc atgactattt tcttcaataa
    20221 aaccaattgt tatttttctg aaaaacaaag ttaactatca gaaaattaaa aaaagtttca
    20281 taaaaatgat tg
//
LOCUS       DADVXO010000045        20242 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.046, whole genome shotgun sequence.
ACCESSION   DADVXO010000045 DADVXO010000000
VERSION     DADVXO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.046"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(71..2146)
                     /locus_tag="KB950_001356"
     CDS             complement(71..2146)
                     /locus_tag="KB950_001356"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBB6857582.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2148..3065)
                     /gene="glyQ"
                     /locus_tag="KB950_001357"
     CDS             complement(2148..3065)
                     /gene="glyQ"
                     /locus_tag="KB950_001357"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBB6857583.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3449..4261)
                     /gene="recO"
                     /locus_tag="KB950_001358"
     CDS             complement(3449..4261)
                     /gene="recO"
                     /locus_tag="KB950_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBB6857584.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4404..5303)
                     /gene="era"
                     /locus_tag="KB950_001359"
     CDS             complement(4404..5303)
                     /gene="era"
                     /locus_tag="KB950_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBB6857585.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5318..5713)
                     /locus_tag="KB950_001360"
     CDS             complement(5318..5713)
                     /locus_tag="KB950_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBB6857586.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5697..6173)
                     /gene="ybeY"
                     /locus_tag="KB950_001361"
     CDS             complement(5697..6173)
                     /gene="ybeY"
                     /locus_tag="KB950_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBB6857587.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6190..8382)
                     /locus_tag="KB950_001362"
     CDS             complement(6190..8382)
                     /locus_tag="KB950_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBB6857588.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8406..9377)
                     /locus_tag="KB950_001363"
     CDS             complement(8406..9377)
                     /locus_tag="KB950_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBB6857589.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10083..11462
                     /gene="brnQ"
                     /locus_tag="KB950_001364"
     CDS             10083..11462
                     /gene="brnQ"
                     /locus_tag="KB950_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBB6857590.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11618..12064)
                     /locus_tag="KB950_001365"
     CDS             complement(11618..12064)
                     /locus_tag="KB950_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBB6857591.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12091..12267)
                     /locus_tag="KB950_001366"
     CDS             complement(12091..12267)
                     /locus_tag="KB950_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBB6857592.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12429..12857)
                     /locus_tag="KB950_001367"
     CDS             complement(12429..12857)
                     /locus_tag="KB950_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6857593.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12949..13809
                     /locus_tag="KB950_001368"
     CDS             12949..13809
                     /locus_tag="KB950_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBB6857594.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13893..14243)
                     /locus_tag="KB950_001369"
     CDS             complement(13893..14243)
                     /locus_tag="KB950_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6857595.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14259..15986)
                     /locus_tag="KB950_001370"
     CDS             complement(14259..15986)
                     /locus_tag="KB950_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBB6857596.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16199..17125)
                     /gene="trxB"
                     /locus_tag="KB950_001371"
     CDS             complement(16199..17125)
                     /gene="trxB"
                     /locus_tag="KB950_001371"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBB6857597.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17270..18706)
                     /locus_tag="KB950_001372"
     CDS             complement(17270..18706)
                     /locus_tag="KB950_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6857598.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18703..20106)
                     /locus_tag="KB950_001373"
     CDS             complement(18703..20106)
                     /locus_tag="KB950_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6857599.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         5456 a         4402 c         3617 g         6767 t
ORIGIN      
        1 atttataaca aataaaccag agaaatagcc tattttgtgc ctgttctctg gtttatttac
       61 atattagaaa ttattttgtg atgatttgat ctaaacttgc tagtgacaaa gccatggcag
      121 caatttgact taattgcgtt aatcggttct tacgtactgc tggttcttct gccatgacca
      181 tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct tacaagctga tcataatttt
      241 cgttgatcgt ttggtcagca aagcttgttt tcattgcctc tactgctttg tgcagttctt
      301 tctccgcttc gttttcgaac agttcagggt ctacttctga ttgtttttca agctccgctt
      361 ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga tggtttgaag tcttcatctg
      421 caagatgctg cttgaacaat tgtgctgctg caaatacttt tgtcagatct ttttgttctg
      481 cattcaaaac agcttcgatg acatcatgac ggatattctt cgttgtcaat aattggcgca
      541 gacggccttt gataaagtcg atcacctgtt gttgtcccgc tgacaagcca atgccaaaac
      601 gatccgtatc tttactgata acttcactaa tcgtttcttg cagaacagac aaaggaaagg
      661 cccatttttt cgattcgatg attcgaacga caccatatgc ttgacggcgc aaagcatatg
      721 gatcgtttga gccacttggg atcatcccaa ctgcaaagaa agaaaagata ctatctaatt
      781 tatcagcaat cgctaaaaca gaacctactg tagtctcagg taaatctcct tcactggata
      841 taggcatgta atgttcacgg atcgctgttg ctacggctgg tttctcgcct ttcagcagag
      901 catatttttc tcccatgatc ccttgtagtt cagggaattc tcctaccatg ttggttacta
      961 gatcaaattt atagatttca gcagcgcgtt gcagatccat caattcttcc tcagatagtc
     1021 ctacttgttc gcctatgatg cgagcgatga cttgtacccg ttgcattttc tcatagatgc
     1081 taccgatttt ttcatggaaa gtaacagttt ttaatttttc aacgtattgg tcgattgaag
     1141 ctttcttgtc ttcattataa aagaattccg catcttctaa acgagccgtc aatacttttt
     1201 gattgcctcg taccacattt tccaatttta cccgatcgcc attacgagca gaaataaaat
     1261 gaggaagcaa cataccttga tcgttccgta cttcgaaata acgttgatgt tctttcatag
     1321 aagtgactag tacttcttca ggaacagata gatatttttc ttcgaagtcg ccagaaaaga
     1381 cagttggata ttcgactaaa ttgttgactt cttctagtaa atcttcatct agatcaatga
     1441 tccagtgatt tttttcggct aattcgtttg cttgtgtgat gatcatttct tttctttttt
     1501 gcggtgaagc aataacaaat tgttcattca atttttcttc gtattctttt gcatgcgtaa
     1561 atgtcacatc ttcgcctaag aaacgatgac ctcttgaagc tctgtctgtt ttgacatcca
     1621 agatctcaaa cgggattatt tcgtcatcta acaatgccac gatccagtga atagggcgaa
     1681 tatattcaaa atcgtattct gcccagtgca tcgttactgg gaaagtgagg cttttaatga
     1741 catctgctaa acctgtcaat acttcttttg cttctttgcc gtgagtgtat ttcgttacat
     1801 agacatattc cacgcctttt aattctctaa aagtgatagc atctgtcgtc agaccttgtc
     1861 cgcggacaaa tccttcagct gctttcgtcc agtttccatc tgcgtccaaa gcaatttttt
     1921 tggctggtcc tttgacttct tcttcgctgt ctgcttgacg ttctggaatg tttgttactt
     1981 tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt atcataagtc aattggtttt
     2041 catctaaaaa ttttgctgtt ttttcttcta attgtttcat gcttggtgtg acaacatgag
     2101 caggcatttc ttccagcccg atttcaagta ataagtcttt tgccatctta ttcagcctcc
     2161 ttcatgtttt ctggatttgc tgtgttttta ttcaataaag ggaatcctaa tttttcgcgt
     2221 tctgccacaa agattttggc tacagcacga gccatgttgc ggatacggga aagataacct
     2281 gctcgttctg ttacggatac tgcaccgcga gcatctaata aattgaatgt atggctgcat
     2341 ttcaaaatat aatcatacgc tggatgaaca agattttcat cgatacagcg tttagcttct
     2401 ttttcaaaac ggtcaaagtt gcttagtagc aagtcttggt cacttacttc aaaagaatat
     2461 ttggaatgtt catattctgg ttgtttgaag atctcgccat atttcacacc atcagcccat
     2521 tccagatcat agacactttc aacttcttga atatatgaag caagacgttc tagtccataa
     2581 gtgatctcag aagtaactgg tttacaaggt agtcctccga cttgctggaa gtacgtgaat
     2641 tgtgtgatct ccatcccatc aagccatact tcccaaccca caccggcaca ccccatcgat
     2701 gggttctccc agttgtcttc aacaaaacga atatcgtgtt ctaatggatc aattcctaaa
     2761 agacgcaagc tttctaaata aagctcttgg atattttcag gtgaaggttt cattacgact
     2821 tgaaattgat gatgctgata caaacggttt gggttttctc cataacgccc gtcagctggt
     2881 cggcgggatg gctctacata agcagcattc catggttccg gtccgattgc ccgtaagaat
     2941 gtatatgggc tcatcgtacc tgctcctttt tctgtgtcat aagcttgcat cagcatgcag
     3001 ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat cttggacggt caatttttta
     3061 ctcatctgtt ttgccccttt caatttttaa gcgttgctgt caataagaat acaggaaaga
     3121 aaacgacaaa aagtccctat gtctgattac agacataggg acgaataatt tcgcggttcc
     3181 accctacttc cgaataaaga ttcggcagct tcatttatcg tactcgcaag tgcctcttca
     3241 aaaatcgttt gggtccggct tccactctcc cgaactcgct aaaccacgtg ttttttactg
     3301 atcttggtca atgtacgtat tcattttgca gttttcttgt ttatataata gatcctctca
     3361 gtgaacgatc tatttaatct acggttaata atagacggaa gtccctgtat ttgtcaaata
     3421 gttttgattt ttttgttgta tctttcattt attcttcttt ttcaggtatc tctttttcag
     3481 gcatattttt ctctggcgtc tttaatacgt gtgcccaaga cttcatttga tcaatgaact
     3541 ttttgctttt tagatgaata ccgacatatt catcatacaa ctggtcaatg aggtcacgga
     3601 ttgcctgctt cgtttcttct ttcaagtgga ttccactgat cttatcataa gaaatagccg
     3661 aaaacattcg aacgaaataa acagcgcgag gatctgcatg ataacggtgt tcgtccattg
     3721 gccagtgttt ctcgcatatc acaccgccag atttggaaga atagtcaaat tttccttttg
     3781 tttccccgca tacgacacag tgcgtccaaa ttggcgcgat tccgaagcgt tgcagtattt
     3841 gaacttcaaa gatattcgtg atgatttctg catcatctcc ttgatccatc cgctgcagag
     3901 cttgttgcgt aaattggaat aaatgcgggt catattgatg atcttcgatt gctgcatcta
     3961 ctagatttag caaataggta ccataaccgc tcaaaaagat atcttcttgg atgttccgaa
     4021 acgcatggac gtctttgaca ctgcttaaaa acgacaaacc ttcacttcga aaattaccga
     4081 tatagactgc ttcggtaaag gggagcaaag ccggagctaa tggctgattt tgccgatgga
     4141 ttcctttgat ataaaacatc attttgccag aagattccgt aaagattttg actaatttat
     4201 ctttttcttt gtaattacgg ctagataaaa tcaagccttt cgattctgca agctggctca
     4261 tattctccct cctaaaaaat aaatctggaa aagcagattc tgccgatcca gattatttat
     4321 gtttcgatct tccgccgttt ttctcaacgt gcggattttt atccagttta tttctatact
     4381 taacctataa gaagttcaga atatcagtat tcgtcttttc ggtagccaaa gtcttgcaga
     4441 tagacttttt tgtctcgcca gtctttttgg actttgaccc aaagttctag aaatactttg
     4501 tctcctaaaa gattttcgat atcctttctt gctttcgttc ctacttgctt cagcattttt
     4561 ccgcctttgc cgataatgat tcctttttgg ctatctcttt cgacgatgat cgtggcctgg
     4621 atatggattt tatcattctc gtttcgcttc atcgaatcaa caacgactgc gacagaatga
     4681 ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga tcaattcaga aacgataaag
     4741 tattcaggat gatcagtcac ttgatcatct ggaaaatatt gtgggccctc tggcatttca
     4801 ctgactaata catccattaa gcgttcgaca ttgtttcctt ctgtagcaga gatcggaatc
     4861 acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga ttcccagcaa ctcgtctgga
     4921 tggatcttat cgattttatt gataatcaga taaactggag aatgcacgtt ttttagacgt
     4981 tcaataataa aatcgtctcc ccggccgcgt ttttgatcag cactgatcat aaataagact
     5041 gcatccactt ctttcaacgc actgtaagct gcttctacca taaaatctcc tagacgatgc
     5101 tttggtttat ggattcctgg tgtatcgata aaaatcagct gagcttctgg tgtagtatat
     5161 actccttgga ttttatttct agttgtctgt gccttgtcac tcataatggc aattttttga
     5221 cccacgattc ggttcaataa tgtggatttt cctacattcg ggcggccgac gattgcgaca
     5281 aaccctgatt tatgttctgg catatacgtt ctccttttta attaaacaac aaatgatata
     5341 ttttagggac aaataaaatc aacccaatga tgacagcaaa acaagcggtc actaaaactg
     5401 ctccagctgc catatctttg atttttttcc caatcggatg aaaatgaaaa tccgtaaaca
     5461 tgtccactac attttcaaaa acagtattga tgatctctac tatccaaacg agaaaaacag
     5521 ctaaaagcag ccataaccac tctatccgat caagctggaa aatcacgccc ataaataaag
     5581 caaggatacc gaacgcaaca tgtttcctca tattgcgttc ttcctcaaaa acggttttga
     5641 ctccttggac agcaaattcc acagacatca ggaaatgttt gtttttctct acgtttttat
     5701 cttttgagtc cataggcatc caagatctct ttctgtaaac caaacatttc tttctcatct
     5761 tctggtgtca tatggtcata cccgttgata tgcaagaatc catgtaccgc taaaaagcca
     5821 agttcccgat catatgaatg gccgtaagct tctgcttgtt cagcagcacg ttcaatagaa
     5881 atcatgatgt ctcctagtac acgaggcaaa ggattctctc catcttcaaa aatcactggg
     5941 atttcgtctt cgccttcttc ttccagagca aaactgatga cgtctgtcgg tgcatctttt
     6001 ccacggtaat cacgattgat tacttggatt gccgcattgt ccatgaaagt aacagacatt
     6061 tctgtttcct ctggcagctt taaataagat gctgcaaatt gcagcaagtc atcgatttct
     6121 ttcattttct cttctgagac ttggtttgtt tcatccataa aggtaatatc cattatacat
     6181 cgcttctttc tattcttctt gttcttcctt tatcttctca acttctgttt tcattttagg
     6241 atatttgatc cgagaatgga aggtgctact taaaccgctg atcaatgatt tttccacttt
     6301 tcggatttct ttcaaggtca gtccactatc gtctaattga ccatcggaaa tacgctcttc
     6361 gataagatta tggacaaaac tttcgatttt ttcgctcgtc ggatgatcca ttgcccgtac
     6421 tgcagcctca caactgtcag caatattgac gatccctgct tcacgacttt gcggttttgg
     6481 tccaggataa cggaattgct cttctgttac ttccggattg cgttctttcg ctttgatgta
     6541 gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa atatcgatca ccatttgcgg
     6601 catattataa tcttccaata ttttggctcc atctgtcaca tgtccaaaga taatatgttt
     6661 actgtcttct ggcaataaga aattgtgcgg gttttccgct cctgaaggca agttctcaac
     6721 gaaaaagcta gcatgtttga tcttgccgat atcatgataa tagcaagcta ctcgagttag
     6781 caaagaacgt ccgccgattt ctgctacagc gttagcactt agattagcta ccatcatact
     6841 atgatgataa gtacctggcg cttcttccag caactgtttc aataaaggat gatttggatt
     6901 gctgagttca tttaatacaa ttacactgtc atcattgacc atcaattcaa tatatgggtg
     6961 tagaccgatc gtcagcaaga atgagaagag tgcaccagtg aacccgcaga caagcattaa
     7021 ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg atcaaaatga catccatcat
     7081 cacaggaaaa ataatgaccc acatggcagc agataatcct tgttctgaga tccgtttgcg
     7141 tctgacaatc gttgccatga gcccagagaa aacataagac atcaagataa tcgttagaaa
     7201 gttcgtgcca atcgaatcat aaaaaatgaa aagtgcagat actgcttgga aaataccagc
     7261 catgatccct gctcttcggt tcaagaaaaa gcttaaaaca agtggtgcaa aagcagcggg
     7321 ataaaacagc gggatataag tagcttgttc cgtttggaat aattggaaaa atttcatcag
     7381 cactatgctg actgacatag ctgtcacata gaaaagcacg tattttgttc tttctcctgc
     7441 attttggaat tgaagtgaat tataaatcag tacgccgatt tgaagcaaaa cagctaaaac
     7501 catggcaatg agtgggaaaa tcgaagtcgt ttgattcgtc aatcctagta attccaattt
     7561 tttcatcgct gaagcatcaa tttgatttcc ttcgcgaaca atgatttctc cttggaagat
     7621 catcactggt tgtacagctt ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt
     7681 caatacatca ttgactacaa tgccttgatc cactaagtag cgaatcattt gttgctgctc
     7741 tgttgtgaca ttcaaatact gaatctgatc ttctgcttct tgtttaaaag cttccaattc
     7801 gctttcacga acttgtttac tcatctgttc gtcaatgagc ttcaaacttt catccctaac
     7861 cgtgtgaatt tccgtttcag tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt
     7921 ttggtaaaac gcaacgtttt ctgcattcac gttttcaaaa tttttcttca gtgtagcaat
     7981 ccgctcttct gtagtaggtt tcgggatatt ttcatcttcc ttcgccttat ctaccttgtc
     8041 attataagat ttgttgatgg tatcattcgt cttatcgatc aattcgaata attgcttgat
     8101 ccgattgttc tggtcatctg ccagatcttt ttgataagta tattctggag tcactgcttc
     8161 tgctgccagt tttcttcttt gttcggtttc ttccgtgttc tcgatcgttt tattcgcacg
     8221 gatactctct tctgctactt ggccttctct gaaatcattg gccttttgtt tgacactact
     8281 aaatgtaacg ccgcagacaa tgataaaaaa caagaacaga aggaaaggca atgctgcctt
     8341 tccgagtttt tcttgtagtt tctgcagtgt tttattcaac atgtgcttat cctcctaagc
     8401 aaagcttatt cttgatgaag atccgccttt tcataagctt tgatgatttc ggcaactaca
     8461 gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt
     8521 agtgttcgtt ctgcatgaac aagaccactt gtcgtccctc gaggcaagtc tatctgactt
     8581 gtatccccat tgacaatcat ttttgattga taaccgagac gggtcaagaa cattttcatc
     8641 tgagcgactg tcgtgttttg tgcttcgtcc aagataacga atgcatcatc caatgtccgc
     8701 ccccgcatgt aagctaatgg tgcgatttcg attacgcctc gttccatcaa tcggttcgta
     8761 tgatccaatc caaaaatttg gtataaagca tcataaacag gccgcagata aggatccact
     8821 ttttctttca gatccccggg tagaaagcct aggttttccc ctgcctctac agcgggccgc
     8881 gtcaaaatga ttttttgcac ttcgcctttt tttagggcag caattgctaa tactacggca
     8941 agaaacgttt tccctgttcc ggctggccct acaccaaata cgatgtcatg tttggctacc
     9001 gattcaacgt atttcttttg accgctgttt tttacccgta taggttttcc attacggtct
     9061 ttgacgatct cttcctcata catctcaaca aaataatcta atgtaccttt ttgccccatt
     9121 ttcaatgccg tcacgacatc tggtgtactt acgtggattc ctcgtttgat caattcctgt
     9181 aatgtccgaa tgactgaggc agccaaggaa atctgggatt gttcaccaat gatctgaatg
     9241 atttctccac gtgtatggat ggttgtttcc gttgtttcct cgatcagttt tatatgcttg
     9301 tcgtgtgcac ctaaaagcat gctgacatca tcagcagctg tcaatctaat ctctaaagaa
     9361 ctagactctt ccgtcaaaaa tagacatccc ctttttttcg attcattctt tcctatttta
     9421 ccatactcag aagaaactat atagcaaata agaaaaggtt gtaaaagagt cgtttaacca
     9481 tacgtaccaa gaaacgttaa aaaatagctg tttccattcg ctaaaggtct cgttttgata
     9541 aaaagaatct gagaatgtaa gttatatgaa agtggagaac aaaatattcc gtttcattca
     9601 gaggtattct ctaggtattt ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt
     9661 cttgcatttc aaaaaaaaca ggtgtattat tacgtcaaca aataaacata cagcaaagcg
     9721 tcgaggaaaa cagtaggcta ttcaacgatt tattcccagt gagtcatgta agtgagaaat
     9781 gacaataaat cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg
     9841 tggctgcctc cattatcagg ctagcggata actttatcaa aaagcgtccg tgaatgaaga
     9901 agctttttta agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg
     9961 gaaaagggct tttttttata ttttaaggtg gtggttttca tggaatctga tattagtggc
    10021 attactaatt gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat
    10081 ttatggaaga aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct
    10141 tcgggatgtt tttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg
    10201 gcgcaaactg ggtcagtgct ggcgtaggat ttgccatctc aggcgctctt ttcccattac
    10261 tagctatctt agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg
    10321 tcggaaaatg gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt
    10381 tcggtacacc acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg
    10441 aaaaatataa ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt
    10501 tccttactgt ccatcccaca cggttggtca aatacgtcgg aaagtatcta aactccttgt
    10561 ttcttgcatt attagcaatc atttttgtcg tttctttatt ccacccaatg ggaaatctaa
    10621 atcaatctgc tgcagatact taccaaacaa acgctttaac aaatggtgtc ctagaaggtt
    10681 ataacacggt tgatgcagta gccttgcttg ccttaagtgt cacttttgtc catgcagtcc
    10741 gtgaccttgg tttcaaagat aaaaaagtca cagaattaac agcaaaagct ggtagcctga
    10801 gcgttgcgct agaagtcatg atctattttg gtcttgttct tctcggtgcg atgagtttga
    10861 ataagatgtc tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt
    10921 taggtaactt cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg
    10981 ctatgggact cgttgtttct tttgcacaag atttccataa actgttccct aaagtcagtt
    11041 atatgacatg gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttgg
    11101 acaatatcat ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt
    11161 tgattttgct ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa
    11221 cgatgttgtt tgctgctgtt cccgccgtat tggatatgct ggcaaattcg cctgctttag
    11281 tgagtcaaca aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg
    11341 ctgcattggg acttggctgg atggtcccaa cgttacttgg gtatgctggc agcttactct
    11401 tctattacgc ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat
    11461 aaccagatca aacacaattt aacataagaa atattaaaca ggaaaaaaac agctagatga
    11521 aagaatccta ttctttcgtc tagctatttt ttagatctct tttttacgag cacaaaaaaa
    11581 ctgcggacaa tgattgtccg cagtttaaat gataaaatta agattgtaat agttctttca
    11641 caatggcatt tacttggttt ccatctgctt ttcctttgac tttaggcatg atcgcaccca
    11701 tgactttgcc aaattctttc attgatgaag caccagtttg cgtgattgct tcttgaacaa
    11761 gttgtcgaat ttcttcgtcg gaaagttgtt ccggcatgta tttttcgact atcacaattt
    11821 cactttttac tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat
    11881 cacgtcgctg tttcatttcg cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt
    11941 gaccttcttt aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag
    12001 attccttatc tttcgctttc atcgctgttt tcatgtcatc gtttaatcta cttagtagtg
    12061 acacactacc aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc
    12121 agattttttc ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg
    12181 taaagtaccc gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc
    12241 gtttttgcga acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac
    12301 cgtaattgtt attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa
    12361 tgctgttttg accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat
    12421 ttaaatcctt attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc
    12481 gtacatccac cgagctgttc ttcgaaataa ttcattggac acatatggat ttctttcgtt
    12541 gttccgcaca ccgtacagat aaagtgatgg tgatggccga tttccccttg acagcaatga
    12601 aaacgaaact tcatctctcc atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa
    12661 tcatggagat ttcgataaat cgtatcgtaa cttactcctt tgaattcctg attcatatag
    12721 tcaaaaactt ctttagcgga tacgtagcga ttgcgcttca ctaaataagt taacaatgct
    12781 tctctttttt tagtatattt aaaaccgtta tttttcaatg tttctagtga ttgttcgatc
    12841 aacgtctgtt ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacacattct
    12901 agagtatggt ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat
    12961 tctacagaaa tcgttacttt ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa
    13021 ctagcgcaag ccactatggc ccaatatcct agcgttgagt tcaatttatt ccgcagaaca
    13081 tttgtgacag atgagaaaac attgatgcag gcactgcaag atgcactaga agaaaaagct
    13141 cttgtcctgc acacattgat caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa
    13201 gaacatcaat tatttagttt tgatgtgatg actccgccga tatcagaaat cgagcgctta
    13261 actggtatca agcccattcg gcaacctggc gcccttcatt tattgaatga gaactatttc
    13321 aaacggatca aagcaatgga atttgctgta aaatacgatg acgggaaaga tcctcgtggg
    13381 tttctagaag cagatgtcgt tttgctaggc gtatcccgaa cttccaaaac gcctttgagc
    13441 ttgtttttag ctaacaaaaa tttaaaagtt gccaatctac ctttgatccc acaagcacat
    13501 attcccaaac agctttggga gatcgatcct aaaaaaatcg tgggactgac gaataaccca
    13561 gatattttaa acaatatccg taaagaacgg atgcgctctt acggattaaa tcctgatact
    13621 gcttattccg atatcgaaaa aatacgagct gaactcgatt ttgcgaatga tctttatgaa
    13681 aaattgggct gcgtagtaat caatgtcgct tctttatcta ttgaagaaac agcttcgctt
    13741 attttgaatg ctttagacct agaagaccat agttattatg gaactgaaac caccgaagaa
    13801 acagactaac aatttttctt taaaaaaact ggactgtgtc agcgaagaag attcgttgac
    13861 acagtccagt ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagtgtc
    13921 tggttcaaaa tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca
    13981 cgatgggact tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat
    14041 tgttccattt ccactgcttg gatgattgct gtcacatttt gttccccgtc tttcagatac
    14101 agcagaattt tgagacgagt ggggtcactc aacattttga atagttggct gactttctct
    14161 acttttgttt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt
    14221 ctaaagttta ccactttttc catcagtctc aagttttttt attcttctgt catcttttgg
    14281 ataatcgttt cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca
    14341 ccgatataaa atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt
    14401 tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct
    14461 tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca
    14521 aattctgttg gtgcttcttc acggaatttt tccatcaaag cagagatttt cgcagaacct
    14581 tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt agtagccata ggtttcaaaa
    14641 atcgtttgca agccatcata caacgttttg ccttgttttt tatagtaagc tgctacttct
    14701 gctaataaga gcaatgcttg gatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa
    14761 ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga
    14821 attttttcag caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt
    14881 tccgcaattg ctgtaggtaa ttcgctagaa acgatcgatt tcaataccgc tgcgttttca
    14941 ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag tgctccgatt
    15001 tggtttccgg tcaatacttc ataggagcca tttggcaaac gaacggctgc acctaaacgg
    15061 tctgcatcag gatctgttgc aatcagcaaa tcagcgcctt ctttctcgcc aagtcgtatc
    15121 gcatactcaa aagcagagtg ttcttctgga tttggtgatt tgactgtagt gaaatctggg
    15181 tctgcgacag cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca
    15241 cgttcgccca acatttttcc tgttccgtga agtggtgtgt acacaagttt caaatctttc
    15301 cccattgttt ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg
    15361 gcatccactt cttctccgat gatcgtgatc aatccgctat gttcagcttc ttcgtcagat
    15421 aatacttcaa cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg
    15481 tctgcaggag gcatttgtcc cccatcttct ccgtagactt tatatccgtt gtaagctgct
    15541 ggattgtggg atgcggttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa
    15601 gaaagctctg gtgttggacg tagactttcg aatacataag atgggatatt gtgatgtgct
    15661 aatgttttgg ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata
    15721 gcgacacctc ggcgttttgt ttcttcgcct tgtgtatcca tgaaacgtgc aagtccttct
    15781 gttgcttgtc ttaccgtata gatgttcatt cgattgatcc caggccctaa gattccccgc
    15841 atgcctgcag taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct
    15901 tcacccagat catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat
    15961 tgttcataca cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta
    16021 ataagtatag cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca
    16081 agaaaggatt cttttatcaa gcacggttgc aagtattcgc ataaatcgga ttcagtagat
    16141 ttcacgcgaa aaaaaagagt aaaagaacaa ttaaaccgtt cttccactct ttttaaactt
    16201 atgcagttgt ttcttctaaa gattccaagt atttatacac ttgttgtcca gcgatcccac
    16261 catcaccaac agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa
    16321 aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg aatccagcca gcttcgttcg
    16381 tcaaccctgc ttctctaaat ggtgcagtta atggatccag tccaacatag atgaatactc
    16441 cgtctgctgc caaatcaaac tcattgccgg ttttgacatt acgtgctttc acacctgtca
    16501 ctaccatttc attcccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt
    16561 cattcgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga
    16621 cgatgactac ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc
    16681 ctcctccgat cactactaga cgtttgttac ggaaaaacgc accatcacaa actgcacagt
    16741 aagaaacgcc acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac
    16801 agcctgttgc gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt
    16861 tgtggctgcc gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc
    16921 ttgaaacacc ttcatacatc ttataagcta gatcaggacc cattattgaa tcgaaaccag
    16981 gatagttttc gatttctgcc gtattgttca tttggccgcc aggggcgcct tgttcgatca
    17041 tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggacctg
    17101 cgccaataac gattacatca tacatatgta gattctcctt tacttaatca agtaagcata
    17161 ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat
    17221 tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat caatcattcg
    17281 aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg
    17341 aacgggctaa tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt
    17401 tcttcatctc acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta
    17461 atagatcgac tccatctaat tctactgcat ctttcattgc ctggatatgt gctgccagat
    17521 agtcgattcg atagtcatct tccacataac cattctcatc gggcgtatcg actgcaccta
    17581 agccgttttc tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta
    17641 ttctcaagcc taatggatca atttgccaac cccattcact tgcttccaga tatggatttt
    17701 tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa
    17761 ctcgagaaga ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg
    17821 catccccagg ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag
    17881 caggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgattttc
    17941 tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca
    18001 tacagcctac tttgttctcc ggatctactt catgagcaat cttcgttgcg atggcacttg
    18061 caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa
    18121 aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca
    18181 accaatattt gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa
    18241 aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc atcggacaat
    18301 caaaatgtgt aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat
    18361 cctcataaaa tttcaaacca gattcatttg gctctttttc atcgcccatt ggaaaaatac
    18421 gactccaagc aatggacatc cgataagtct tgaaccccat ttctgcaaat aaagcaatat
    18481 cttccttata atgatgatac atatcgatcg caatttttgc tggataaaaa tgattttcat
    18541 caaattcaaa cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca
    18601 caacatctac gttcgctaac ccacgtccgc cttcatcata accgccctcg cattgattgg
    18661 ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac
    18721 atgtgtattg cttgataatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt
    18781 taaccggctg attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc
    18841 attcatgatc cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg
    18901 taaagctgtt ccataatcac cagtcacagc tgcttcgatc gtcagcaatt ccatattctt
    18961 cattacttgc aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc
    19021 gccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac
    19081 agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat agatcgaagc
    19141 aatcgtttca caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg
    19201 tttatgatcc aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac
    19261 ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc
    19321 ttcacggtag taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc
    19381 tttgaaaaac gggatatcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg
    19441 atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa
    19501 gtgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgttgg
    19561 ttctgtcatc atcgccatta ctggcacatt gcataatcct atcactttat cccatttgcc
    19621 atatctgata actgcttctg tcaccatccc gcttgggttc gcgaaattga tcagccaagc
    19681 atttggacat aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa
    19741 agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg
    19801 gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa
    19861 atccgcatct ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg
    19921 agaagcatcc cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc
    19981 ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc
    20041 ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt
    20101 tgccatgttc tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa
    20161 acaatagaaa ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt ctactggatt
    20221 tttgtaaata tcttgtcaaa aa
//
LOCUS       DADVXO010000046        19987 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.047, whole genome shotgun sequence.
ACCESSION   DADVXO010000046 DADVXO010000000
VERSION     DADVXO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.047"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            22..834
                     /locus_tag="KB950_001374"
     CDS             22..834
                     /locus_tag="KB950_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBB6857600.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            831..1631
                     /locus_tag="KB950_001375"
     CDS             831..1631
                     /locus_tag="KB950_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBB6857601.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1792..3060
                     /gene="rseP"
                     /locus_tag="KB950_001376"
     CDS             1792..3060
                     /gene="rseP"
                     /locus_tag="KB950_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBB6857602.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3128..4837
                     /locus_tag="KB950_001377"
     CDS             3128..4837
                     /locus_tag="KB950_001377"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBB6857603.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5049..9407
                     /locus_tag="KB950_001378"
     CDS             5049..9407
                     /locus_tag="KB950_001378"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBB6857604.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9552..10025
                     /gene="rimP"
                     /locus_tag="KB950_001379"
     CDS             9552..10025
                     /gene="rimP"
                     /locus_tag="KB950_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBB6857605.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10048..11223
                     /gene="nusA"
                     /locus_tag="KB950_001380"
     CDS             10048..11223
                     /gene="nusA"
                     /locus_tag="KB950_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBB6857606.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11245..11538
                     /locus_tag="KB950_001381"
     CDS             11245..11538
                     /locus_tag="KB950_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBB6857607.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11535..11846
                     /locus_tag="KB950_001382"
     CDS             11535..11846
                     /locus_tag="KB950_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBB6857608.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11859..14165
                     /gene="infB"
                     /locus_tag="KB950_001383"
     CDS             11859..14165
                     /gene="infB"
                     /locus_tag="KB950_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBB6857609.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14192..14539
                     /gene="rbfA"
                     /locus_tag="KB950_001384"
     CDS             14192..14539
                     /gene="rbfA"
                     /locus_tag="KB950_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBB6857610.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14696..14869)
                     /locus_tag="KB950_001385"
     CDS             complement(14696..14869)
                     /locus_tag="KB950_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6857611.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15317..17679
                     /locus_tag="KB950_001386"
                     /pseudo
     CDS             15317..17679
                     /locus_tag="KB950_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18047..18970
                     /gene="truB"
                     /locus_tag="KB950_001387"
     CDS             18047..18970
                     /gene="truB"
                     /locus_tag="KB950_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBB6857612.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18974..19915
                     /gene="ribF"
                     /locus_tag="KB950_001388"
     CDS             18974..19915
                     /gene="ribF"
                     /locus_tag="KB950_001388"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBB6857613.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6851 a         3518 c         4387 g         5231 t
ORIGIN      
        1 ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa atatattcaa
       61 gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat tgccatcatc
      121 atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa
      181 gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg
      241 ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga agtaagtttt
      301 ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat taaagaaaat
      361 gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca agatgctgta
      421 agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt tgctttgaat
      481 tatggaagca gagcagagat cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa
      541 ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt aatgacaggt
      601 ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg agaagagcga
      661 attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac aaaagcgctt
      721 tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca aaatcgagac
      781 cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc atgaaacaac
      841 gtgtggtcac agcagccgtg gcgctagtct tgtttattcc gattatatgg atcggaggca
      901 tagcggttga gcttacagca gcaatcttag cagtaatagg agtttacgag ctgtttcgga
      961 tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt gcagtgttac
     1021 ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt acatttatgt
     1081 tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa aatacgtata
     1141 cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata ggtttccaga
     1201 attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta tttattgttt
     1261 gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat aagttaagtc
     1321 ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtattggt agtgctgttg
     1381 tagtcgcatt tctttattta ttgctatacc cagcaaaaga actatttaat catggtatgt
     1441 ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt gatttggtcg
     1501 aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta ccaggccatg
     1561 gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg catttatttg
     1621 gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac gcaggttttt
     1681 atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa ttatagtgtt
     1741 ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg
     1801 attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt tggtcatttc
     1861 ttctttgcga aacgatcagg aatcctcgtt cgcgaatttg ctatcggtat gggtccgaaa
     1921 atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt acctattggc
     1981 ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc aggaatgcct
     2041 ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag taagaaaatc
     2101 caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga tgagctgact
     2161 atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt tgatcacgat
     2221 gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga cgtacagttc
     2281 caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat gaataacttt
     2341 attttggcaa tcctgttatt catcattctg gcatttatgc aaggtggtgt tcaagtgacg
     2401 aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc aggcttaaaa
     2461 gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa tgatctgaca
     2521 acagtcatta cgaaaaatcc ggataaaaca ttagacttta agatcgaacg agaaggtcag
     2581 gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa agttggccaa
     2641 cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg agggacgcgt
     2701 caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt tacaggtttc
     2761 agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga agcagcaaat
     2821 caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct cggaatcgtc
     2881 aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat ttttgaaggg
     2941 attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc tggctttgga
     3001 tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt ctttttctag
     3061 aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa ataaaggagt
     3121 atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc caaatgatgc
     3181 agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag tatcagcggg
     3241 tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac gcattatgcg
     3301 ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct tgccagcaga
     3361 actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc gtttcaaaga
     3421 ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta ctgatttgat
     3481 gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa tccaacctaa
     3541 atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa
     3601 agatgcgtat tctttccatg caacggaaga aagtttggac gagacatata aagattacga
     3661 aaaagcctac accgaagtat tcaagcgttg tggcttagaa tttcgctcga ttatcggaga
     3721 cggtggcgca atgggcggga aagactcaaa agaattcatg gcgatctcag aaatcgggga
     3781 ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga tggctacaag
     3841 ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag tagctacacc
     3901 taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc aaaaaatcat
     3961 caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac gcggagacca
     4021 tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag aagaagcaac
     4081 agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac cagtcggtgt
     4141 ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga atgccattac
     4201 tggagcgaac gaaacaggat tccactataa aaatgtgaat ccagaacgcg atttcactgt
     4261 actttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag acaacaatgg
     4321 tgttcttgcc tttactcgag gaatcgaaat cggacatatc ttcaaaatcg gcactcgtta
     4381 cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat
     4441 gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac aacattcaga
     4501 tgaacaagga atcaactggc caaaaggaat tgcgccattt gatttgcata tcgtccaaat
     4561 gaatctaaaa gacgaatacc aaacaaacct gacgaatgaa ctagaagaag cgatgacaaa
     4621 agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga aattcgcgga
     4681 ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag tggatggaat
     4741 cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag aagaattggt
     4801 gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac acagaaaata
     4861 aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa ttggaactgc
     4921 gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag tatactaaaa
     4981 ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat cagaaagggc
     5041 gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa atccaactag
     5101 aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt gtagtggtcc
     5161 atcgtaaatc gcgtttatgg gaatttacgc tgcatttttc caaaattttg cctattatgc
     5221 tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca caagtcaaac
     5281 tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat tactgggccc
     5341 aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg aaaacgcagg
     5401 tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga gctatttctt
     5461 atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac ggattcccaa
     5521 aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgagtattg aaactttttg
     5581 aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa agtctagttg
     5641 tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc
     5701 acatccagct aggacgaaat attccagccg atgaaccaac gacaccaatg atcagtatcg
     5761 ttgaagaaga acgaagagtc acacttgagg ggtacgtatt cgacaaagaa gtcagagaac
     5821 tacgttcaaa acgcaagatc ttgactttga aaatcacaga ttatacttca tcatttatcg
     5881 tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta
     5941 gctggttaaa ggtacgagga agtattcaag aagatacgtt tgtccgtgat ttggtcatga
     6001 atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa agattatgct cctgaagggg
     6061 aaaaacgagt agagcttcat gttcatagca atatgagtac gatggatgca acaaacagca
     6121 tctctgatct tgtggcacaa gcagggaaat ggggacaccg agctatcgcc attacagacc
     6181 acggtggtgc gcaagccttt ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa
     6241 tcttatacgg cgtggaagcg aatgtcgtag atgatggtgt cccaattgct tataatgatg
     6301 cacatgaagc attgagtgaa gccacatatg tcgtatttga cgtggaaacg acaggattgt
     6361 ctgcagtata tgacacgatc atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga
     6421 tcgaaagctt tgatgaattt atcgatccag gacatccttt gtcacgaaca acggtagatc
     6481 tgactggtat cacggatgga atggttcgag gatcaaagtc ggaagaagaa gtattgcgga
     6541 tgttccttga attttcaaaa gatacaattt tggtagccca caacgcggct tttgatatgg
     6601 ggttcttgaa tactagctac gcaaggtatg gtattcctga agcggccaat ccagtcattg
     6661 atacgttgga attagctaga tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt
     6721 tatctaaaaa atttggtgtc agcttggaac agcatcaccg agcaatctat gatgctgaag
     6781 caactggtca cttagcatgg atctttgtga aagaggcaat ggacaatcat aacatgcttt
     6841 atcatgatca gctaaatgag catatcggag aaggagattc ttacaaaagg gctcgacctt
     6901 tccatgtaac gattttagca aaaaatcagg caggactgaa agatttgttc aaactgattt
     6961 caatgtcgaa tgtcgaatac ttcgaacgcg tcccacgaat accacgctcc caattgaaaa
     7021 agatgcgtga aaatctattg atcggctcag cttgtgataa gggagaaata ttcgaagcca
     7081 tgatgcaaaa aggggtagaa gaggcaagaa accgagcaaa attctacgat tatattgaag
     7141 ttatgccaaa agcggtctat gcgcctttga tcgagcaaga attggtaaaa aatgaacatg
     7201 atttggaaga aattatccaa aatttggtag aaatcggaaa aagtttggat aaaatcgttg
     7261 ttgcaacagg taatgtacat tatctgaacg aagaagatgc catttaccga aaaattttga
     7321 tcaattctat gggcggagcg aatccgttga atcgtcatag tttgccggat gtccatttcc
     7381 gaacaaccga tgagatgcta actgcatttc actttttagg ggaagaaaca gcgaaagaga
     7441 tcgtagtaga aaatacgaat aaaatcgcag atatctgcga agaagtgatt ccagtcaaag
     7501 atgaacttta tacaccgaag attccaggtt cagaagatga aatatcagaa ttgagttaca
     7561 caaaagcaaa acaaatgtac ggagatccgt tacctgagat cattcaaaaa cgattaaaaa
     7621 aagagttgaa ttctattaat ggtaatggct tctcggttat ttatctgatc gcccaaaaac
     7681 tagtgcataa aagtaatgaa gatggctatc tagtaggttc acgtggctct gttggctcta
     7741 gctttgttgc gacgatgaca ggaatcactg aagttaatcc gttagcccct cattattatt
     7801 gtccagagtg ccaatattcc gagttttttg aagatggtac gtatggttct ggatttgaca
     7861 tgccagaaaa acaatgtcca aaatgtggtg cacgcttaaa caaagacggt catgatattc
     7921 catttgagac atttttagga ttccatggag acaaagtgcc cgatattgat ttgaacttct
     7981 caggtgatta tcaagcagaa gcccataact atacgaaagt attgtttggc gaagactatg
     8041 tttaccgtgc cggaacgatt ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag
     8101 gatacgaaag ggacaataat ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag
     8161 gcgctactgg agtcaaacgg acaaccggcc agcatccagg ggggatcatc gttattcccg
     8221 attatatgga tgtctatgat tttacgccga tccaatatcc agcagacgat caaaattcgg
     8281 aatggaaaac gactcatttt gattttcact cgattcacga taatgtattg aaattagata
     8341 ttctagggca tgatgaccca actgttattc ggatgcttca agatttatca gggatcgatc
     8401 cacaaacgat ccctacggat gacccggaag tgatgcggat atttgccggt cctgaagtac
     8461 ttggagtaag tcaggaacaa atttattcca aaacagggac attaggtatt ccagaattcg
     8521 gcactcgttt tgttcgaggg atgctagaag aaacgcatcc tacaactttt gcagaactac
     8581 tgcaaatttc cggactatct catggtacag acgtatggtt aggaaatgcg gaagaattga
     8641 ttcgacgagg agatgcgaca ctggcggaag taatcggctg tcgtgatgat attatggttt
     8701 atttgatcca tgctggatta gatagcggga tggcatttaa aatcatggaa actgttcgta
     8761 aaggacaatg gaacaagatc cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag
     8821 aaaataatgt gcccgactgg tatatcgatt cttgttcaaa aatcaaatat atgtttccga
     8881 aagcccatgc tgcagcttac gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt
     8941 tccctatttt gtattactgt gcatactttt ctgtacgtgc agacgacttt gatctagtct
     9001 ccatgtgcaa aggaaaagat gctgtgaaac aagcaatgaa agaaatcacg gacaaaggat
     9061 tagatgcttc cgtaaaagaa aagaatcaac tgacagtgct ggagttagcc aatgaaatgc
     9121 tggaacgagg attcaaattt ggaatgattg atttgtataa atcggatgcc gttaactttg
     9181 ttattgaagg tgatacgttg atcgcaccat ttagagctgt cccaagtttg ggaaccaacg
     9241 tggcaaaaca aatcgttgaa gcaagaaaag acggtccatt tttatccaaa gaagatcttg
     9301 ccacccgcgg taaagtttca aaaacattga tcgaatatat gaacgacaat ggcgtattga
     9361 aagatctacc agatgaaaat caattatcgt tattcgatat gctataagca tctgttttcc
     9421 tgtctgcttg taattttaag cagagtatgg tatagttctt ataggtaatg atactaactg
     9481 tgagtgagcg gatttttcgc tcactctttt taatggaatt acgcacttaa ttttttaagg
     9541 aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag
     9601 atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct
     9661 tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg
     9721 agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc
     9781 tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg
     9841 cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg
     9901 aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac
     9961 gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat
    10021 tttaatcacg gagaggaatc agaaaaaatg agtaaagaaa tgttaaatgc gttagacgct
    10081 ttagaagctg aaaaagggat ctcaaaagaa atcgtgatcg atgctttaga agcagcattg
    10141 gtttctgcat acaaacgtca ctatggccaa gcacaaaacg tcgaagtgga atttgatcag
    10201 aaaaaaggaa agatccatgt ctatgcggta aaagaagtaa cggaagaagt gatggattca
    10261 cagttggaag tctcattgaa agatgcactt ttaatcaatc cagcatatga gatcggtgat
    10321 acgatccgtt ttgaagtaac accaaaagat tttgggcgga tcgctgcgca aacggctaaa
    10381 caagttattt tacagcgtgt acgtgaagca gaaagaacga ttatttataa cgaattcagt
    10441 gcttatgaaa aagacatcat gcaaggaatc gtcgaacgcc aggataaacg ttatatttat
    10501 gtcaatcttg gtaaaattga ggcagttctt tctaaacaag atcaaatgcc aaatgaattt
    10561 tatcaaccac atgatcgaat caaagtttat gtttcccgag tagaaaatac gtctaaaggt
    10621 ccgcaagtat ttgttagccg cagccatcca gacctattgc gccgcttatt tgagcaagaa
    10681 gtaccagaag tctatgatgg aatcgttgaa attgtcagca tcgcccgtga agctggcgat
    10741 cgttcaaaag tggcagttcg ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc
    10801 gggccaaagg gacaacgagt tcaagcaatc gtcaatgaat taaaaggcga aaacatggat
    10861 atcgtcgaat ggaatgaaga tccagctgta ttcattgcaa atgcattgaa tcctgcacaa
    10921 gtcatggatg tgatcttcga tgagacaaat ccaaaggcct gcacagttgt cgttccagac
    10981 taccaattat cgctagcaat cggtaaacgc ggacaaaacg cacgtttagc tgctaaattg
    11041 actaaccata aaatcgatat caaatcagaa tctgacatga cggaatttta tgaaaatcaa
    11101 gaacaacaaa aagaaacaga agaactgcat gatgaagcaa tcgttcagtc agatttaaca
    11161 gacgatgaat atgaaacaat tgcattcaac aatgaaacgg tcgaagaaaa accagaagct
    11221 taactttagt aaggaggcaa agagatgaaa caaaggaaaa ttcctttacg caaatccgtg
    11281 gtttcaggtg aaatgaaacc taaaaaagag atggtccgaa tcacccgttc aaaagaaggg
    11341 gaagtatcga tcgatccaac tggaaaaatg cctggacgag gagcgtatgt ctcacttgag
    11401 ccggaagaag tacaacaagc ttgggataaa aaaattctcg atcgagtact cgaaacaaca
    11461 ttaacagatg agttttatca agaacttctt gactatgtca ctcaccaaaa agcacgtaaa
    11521 gagttatttg gaaaatgaat acagttaatc ggcaaaaagc catgaatctc atcggtctag
    11581 cgatgcgcgc gggaaaacta atcacgggtg aagaactgac cattgctgaa atccgcaaac
    11641 aaaaagcaaa attggtgttt gtggcaaccg atgcaagcga gaatacaaga aaaaagataa
    11701 aagataagag ttcatattat gaagttcctt gctttgagct attttctgaa gcagaaatca
    11761 cgcagatgat cggaaaacct cgcaaggtat tcggaataat ggatgccgga tttgcgaaga
    11821 agaccaagga gctaattgaa ggttaggaag gtgattgcat gggcaacaaa agaatctatg
    11881 aattagcaaa agaactcaaa aaatctagta aagacgtcgt ggaaaaagca caacaacttg
    11941 gcatcgatgt gaaaaaccat atgggtgcga ttactagcaa tgatgagaag aaattgcaac
    12001 aagcgttcaa aaacccaagc acgaaacaac aagctgcaca acaagcaagc agaaaaccag
    12061 ccaatcaaca gccgaacgat caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata
    12121 aaaataaccg cagttatcaa gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa
    12181 aaccaacaac aaaccaaaat aatacgggaa ataccagcag ccaaaatcgt caaggaacga
    12241 gccaaggcgg caatcaaaac cgtactaaca ataacaacaa accaaacaat aacaatcgcg
    12301 gcaaatttaa taataataac cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc
    12361 aacaatcaac taaaccagct gtaccaccac gcaaattccg tgaattgcca gatgtattag
    12421 agtatactga aggtatgaac gtagcagata ttgcgaaaaa aattcatcgt gagccagcag
    12481 aaatcattaa aaaattattt atgcttggtg tcatggtcaa tcaaaaccaa gcactggata
    12541 aagatacgat tgaactgtta gcgacggact atggcatgga accacaagaa aaagtccaag
    12601 ttgacattgc tgatatcgat aaattcttcg aggcagaaga agtcgatcca gataaattag
    12661 tctcacgtcc gccagtcgta acgattatgg gacacgtaga ccatgggaaa acaactttat
    12721 tggatacatt acgccattca cgtgtaacaa gcggcgaagc cggcggaatc acacagcata
    12781 tcggtgccta ccagatcgat attgatggta aaccaatcac tttcttggat acaccaggac
    12841 atgcggcctt tacaagtatg cgtgcacgtg gagcaagtat cacagatatc acgatcttag
    12901 ttgttgctgc agatgatggc gtcatgccac aaacagttga agcaatcaac catgccaaag
    12961 ctgcaggcgt tccaatcatc gtcgctgtaa ataaaattga taaaccaggt gcaaaccctc
    13021 aacatgttat gcaagaatta agtgagtatg agttaattcc tgaagcatgg ggtggagaaa
    13081 cgatctttgt tgagatctca gcgaaattcg gccaaaacat cgaagaattg ctggaaatga
    13141 tcctgttagt cgcagaagta gaagatttaa aagcagatcc aacacaacgc gcaatcggaa
    13201 cagtgatcga agcacgtctt gataaaggta aaggtcctgt gtcaacctta cttgtccaag
    13261 aaggaacact tcatgttggt gacccaatcg ttgtcggtaa tacttacgga cgtgtccgtg
    13321 taatgacaaa cgatcttggc cgtcgcgata aagaagcagg acctgcaacg ccagtagaaa
    13381 tcactggctt gaatgatgta cctcaagcag gtgaccgttt cgtagtcttc gaagacgaaa
    13441 agacagcacg tgcagcgggt gaagaacgcg cgaaacgtgc attattagaa aaccgtgctg
    13501 ccacaagccg tgtgactttg gataacttat ttgaaagcct gaaagaaggc gaattgaaag
    13561 aagttaacgt aatcatcaaa gcagatgtac aaggttcagc agaagcactg gctgcatcat
    13621 tgaagaagat tgatgtggaa ggtgtccgtg taaaaatcgt ccattcagct gtcggagcaa
    13681 tcaatgaaag tgatgtaacc ttagcagcag ccagcaacgc tattatcatc ggtttcaacg
    13741 ttcgtcctac accgcaggca aaagtgcaag ctgatactga agaagtcgat attcgtcttc
    13801 accgtatcat ctataaagcc atcgaagaaa tcgaaactgc gatgaaaggg atgctagacc
    13861 ctgaatttga agaaaaaatc accggtcaaa tgatcgttcg tgaaaccttc aaagtttcta
    13921 aagttggtac gattgcaggt gcctatgtaa cagaaggata tattcgtaga gatagcggtg
    13981 tccgtgtaat ccgtgacgga atcgtaatct atgaaggcca acttgcaagt ctgaaacgat
    14041 tcaaagatga cgtgaaagaa gtcaaaatgg gttatgaatg tggtgcaatg attgaaaaat
    14101 tcaatgacat caaagtagat gacgtaattg aaggatttat catggaagaa gtcaaaaatg
    14161 actaaaaact gataaaagga ggccaatcat tatggctaac taccgtgacc gccgagtcgg
    14221 tcaagaaatc ttaaaagaag tcaatgatat tttgcgcaaa aaggtccgtg accctcgtgt
    14281 ggagaacgta acgatcacag atgtgaaagt tacgggggac ttacagcaag caacgatcta
    14341 ttacagcatt ttatctgact tagcatcaga taagaaaaca gcacaagatg gattgaataa
    14401 agcatcagga ttgattcgtc gagagttagg gcacaatcta agtatttata agacacctga
    14461 attgttcttt gaacttgatg aatcagttgt ctatggcaac catatcgatg aattgcttcg
    14521 cgacttgaac aaagactaat caaaaactcc cgcaaatcct gtgaaatcag gggttgcggg
    14581 atttttttgt tttcattaag acgtattacc taagagttcc atgactttag tcttgcggcg
    14641 tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct tttttctagt
    14701 agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt
    14761 aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac
    14821 cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat
    14881 tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt
    14941 agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt
    15001 tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccagcaat
    15061 gtgttaactg gcccagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc
    15121 ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag
    15181 gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg
    15241 aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg
    15301 aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg
    15361 gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata accccttact
    15421 gagacgtaca ctgaaacccg aagatgtcaa aaaacatcca atcggtcatt ggggaacgat
    15481 tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa
    15541 catgttttat attgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt
    15601 agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa
    15661 attattcaaa cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc
    15721 aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt
    15781 attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg
    15841 tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt
    15901 tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa
    15961 atcagatgga gaattagcaa attactttaa cggacttggc tgggaacctt tctttattga
    16021 aggaaatgat tcagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga
    16081 gaaaattaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc
    16141 aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga
    16201 cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga
    16261 acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt
    16321 atttgatgca caaggacgta ttttagaaga aatcagagaa atcgcgccta ctggtgacca
    16381 aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc
    16441 agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat
    16501 gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat
    16561 atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg
    16621 ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt
    16681 catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac
    16741 tggacggcat ggttttttgc aagttacgaa tcattcttgc gagtggttga ctctatgttg
    16801 actcagcact tcaaatggat gcggaaatca catgaccttt cttggcgaaa tgattatcct
    16861 tcgctaaatc tcattgcaag ctcaacagtc ttccagcaag atcacaatgg atatagccac
    16921 caagatccag gaatattgac acatttggca gaaaagaagg cggagttcat ccgtgaatat
    16981 ctgcctgcgg atgcaaatac attgctcgca gtcatggata aagcattccg ctcgtcagaa
    17041 aaaatcaatt tgatcatctc ttccaaacat ccgcgtgctc aattttactc agcagaagaa
    17101 gcagccgtct tagtgaatga aggcttgaaa atcattgatt gggcttcgac agcaaaagag
    17161 gaggaacctg aattggtcat tgctgctgca ggaacggaat ctaacttaga agcattagca
    17221 gctgtaactt tgctgctgga agaattccca aaactaaaaa ttcggttcat caatgtcgta
    17281 gatttattga aattacgtca tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt
    17341 gatcaatatt ttacaaaaga caaaccgatc ttatttgctt tccacggtta tgaaacattg
    17401 gtccgcacga ttttctttga tcgtcataac catcatttaa tgattcatgg ctacaaagaa
    17461 aatggagata tcacgacacc atttgacatg cgtgtagtaa atgaacttga tcgttatcat
    17521 ttggcaaaag acgcagcttt aaaaatcaaa ggaagtcaag cagaagattt tgcgaaaaaa
    17581 atggatcaga aattacaaga acatcaaaac tatattcgtg aaaatggtat tgatctgcca
    17641 gaagtattgg attggaaatg gaaaaatctt gaccaataaa atgataaaat cgatcccgca
    17701 aaatagaatt ttttacattg attaagaaac tgaaaaaata aataaaaaaa acggaaaaaa
    17761 gactggaaga atggccagtc ttttttttcg tttttgggaa gtgaaaacca aactatttac
    17821 ctgtaagcca cccataaatc caatcaaatg gtgcaaagaa aaaatcttcc attctattcc
    17881 ctcctttaaa aaagcataac atgatttcta tttacgtgag catgaatttt gttacgtttt
    17941 tattgccgaa aaataaaaac cgttcattat gatgtcaaat tcgtcagtaa ttgatgcaat
    18001 gtgttataat aattcagtta aatcaaaaat gaaatggaga aaagacatgg acgggttatt
    18061 acctttatgg aaagaacgcg gaatgacaag tcatgattgc gtattcaaat taagaaaaat
    18121 cttacatacg aaaaaaatcg gacatggggg tacgcttgac ccagacgtag acggtgtgtt
    18181 gcccatttgt attggaaaag ccacaaaagt tatcgaatat ttgacagatt ctgggaaaat
    18241 ctatgtaggc gagataactc taggtttcag tacaacgacg gaagatcgat ccggagaacg
    18301 ggtagaaaca aaaccagtag atatgtcttt atctgaaaaa aaaatagatg aagtgatggc
    18361 ttcatttatt ggtgagatca cccagatacc accaatgtac tcagcggtaa aagtcaacgg
    18421 taagcggtta tatgaatatg cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc
    18481 tcagatttac cgttttatcc ggacaagtga attgacaaga gacgaagtga caggaaccat
    18541 ttcttggcgc ttcgaagtag aatgcgggaa aggaacctat gtccgcacat tagctgtaga
    18601 tacaggcaag aaacttggtt accctgcgca catgtctgat ctgacccgaa cagcaagtgc
    18661 cggtatgtcc cagaaggatg ctctgacatt agaagaagta gctcaagctg tgaaaaatgg
    18721 gacaatcgat gattgtttgc tgccaataga aaaaggagtg gaacggttca aacgagtaga
    18781 tattactgat gaagtatggc agaaagtaaa aaacggtatg agacttgatt atactgtatt
    18841 tgcgctccca gaaatgccgt cagaagaaat tgctttgttt tataaaggaa aagttgtcag
    18901 tatttatcag ccaaacccga aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa
    18961 tgaggaataa cgaatgaata tcatcaagat cagacatcct taccaatcag aacaaattcc
    19021 agaagacgat gtcgttttag tgctgggatt tttcgacggc gtgcatatcg gacaccagaa
    19081 agtcataaag acaggtatgg aaattgcaca aaaagaagga ttgaaattag cattgatgac
    19141 tttcaatcag catccatcta ttgtctttaa aaagatcgat cctagcagcg tgaaatattt
    19201 gactagcctt aagcaaaaag aagaaaaaat ggccgctttg ggtatagatt atttatatga
    19261 agttgatttt acttcttctt ttgctcattt agcaccgcag gaatttgttg atcaatacat
    19321 cgtagggtta catgcaaaat atgctgtttc cggttttgat tatacgtatg gaccaaaaga
    19381 cattgcagat gtcgctcatt ttcctgaata tgccaaagga agattcaaaa tcgtcacagt
    19441 ttctaaagaa gaagaacaag gtgaaaaaat cagctctacc cgtattcgtg gattactaga
    19501 cagcggacaa gtaaaagaag cagcaaaact attaggttgc acatatgagg tagaaggggt
    19561 agttgtccat ggcgatgcac gaggcagact actaggattc cctacagcaa atgtcaaagt
    19621 aaagagcacg gttcatttgc ctaaagaagg tgtttatgtg tcggaaataa aaattgggga
    19681 aacatggtat ccagccatgg gttcaattgg acataatgat acattcggtg aaggacggga
    19741 gctgactgta gagttgtata ttttggactt taatcaagat atctatggtg aacatgtcgc
    19801 ggtacgctgg aatcaatttt taagagatca agtgaaattt tctagtgtag aggaattgat
    19861 tgaacaactc aaggaggatg aacaagcaac agctgaatat ttttctcagc aataaaacat
    19921 tatgcataaa atctcttgtt tatgtataac tagcaattac aataaaatgg gtgataaata
    19981 agcccag
//
LOCUS       DADVXO010000047        19922 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.048, whole genome shotgun sequence.
ACCESSION   DADVXO010000047 DADVXO010000000
VERSION     DADVXO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.048"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            107..295
                     /locus_tag="KB950_001389"
     CDS             107..295
                     /locus_tag="KB950_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857614.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
     gene            complement(939..1892)
                     /locus_tag="KB950_001390"
     CDS             complement(939..1892)
                     /locus_tag="KB950_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6857615.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1915..2670)
                     /locus_tag="KB950_001391"
     CDS             complement(1915..2670)
                     /locus_tag="KB950_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6857616.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2667..3626)
                     /locus_tag="KB950_001392"
     CDS             complement(2667..3626)
                     /locus_tag="KB950_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBB6857617.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3619..4569)
                     /locus_tag="KB950_001393"
     CDS             complement(3619..4569)
                     /locus_tag="KB950_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBB6857618.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4719..5171
                     /locus_tag="KB950_001394"
     CDS             4719..5171
                     /locus_tag="KB950_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBB6857619.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5260..7527)
                     /gene="gshAB"
                     /locus_tag="KB950_001395"
     CDS             complement(5260..7527)
                     /gene="gshAB"
                     /locus_tag="KB950_001395"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBB6857620.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7714..8712)
                     /gene="ftsY"
                     /locus_tag="KB950_001396"
     CDS             complement(7714..8712)
                     /gene="ftsY"
                     /locus_tag="KB950_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBB6857621.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8723..9532)
                     /locus_tag="KB950_001397"
     CDS             complement(8723..9532)
                     /locus_tag="KB950_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6857622.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9541..13122)
                     /gene="smc"
                     /locus_tag="KB950_001398"
     CDS             complement(9541..13122)
                     /gene="smc"
                     /locus_tag="KB950_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBB6857623.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13142..13828)
                     /locus_tag="KB950_001399"
     CDS             complement(13142..13828)
                     /locus_tag="KB950_001399"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBB6857624.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14176..15957)
                     /locus_tag="KB950_001400"
     CDS             complement(14176..15957)
                     /locus_tag="KB950_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6857625.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15983..16897)
                     /locus_tag="KB950_001401"
     CDS             complement(15983..16897)
                     /locus_tag="KB950_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857626.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16924..17886)
                     /locus_tag="KB950_001402"
     CDS             complement(16924..17886)
                     /locus_tag="KB950_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857627.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17886..18830)
                     /locus_tag="KB950_001403"
     CDS             complement(17886..18830)
                     /locus_tag="KB950_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857628.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18831..19835)
                     /locus_tag="KB950_001404"
     CDS             complement(18831..19835)
                     /locus_tag="KB950_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857629.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5538 a         4274 c         3544 g         6566 t
ORIGIN      
        1 gaaggaatat ataaaaaaca tctattttat cgaagaaaca caaaatatcg agggttctta
       61 tattgaagta aaaacactct ttgtcaatga agataaaacc aaagcattgg atatttataa
      121 aaaattagct tcaaaaaaaa cgaatagttt tggattgata ttaagcgagt ataaaatcaa
      181 agcagaagag agttatttct atcaattact aaaacggtgg agtaagcttc ccgccgattt
      241 ttatcgaaaa atgcagatca tcaactatca accgcttgct gagacacatg cctaaataga
      301 ggttgtgaaa tcagtgcttt gagctaatac cgaaggtcag ctgattgaac attgtttaga
      361 agagattgtg acaactgcga tttgctttga gtaataagaa cgctggacag aaaatagctt
      421 ctcatatttt ctagtctagt gggcttattg ccgagaagca gcagatggaa catcgtttag
      481 aagagattgt gacaactgcg atttgttttg agtaataaga acgctggaca gaaaatagct
      541 tctcatattt tctagtctag tggacttatt gccgagaagc agcagatgga acatcgttta
      601 aagaagagat cggtacaaaa taaaaatatc taaatttaag ttttatctaa gtttcgcttt
      661 gtacctttga ttcaaatcaa aggaggcgat cttgatgctg aaaaaatgga tcacactatt
      721 ttttgccact ccccattcta gaatcaaccc gtatgatgta gataatgaat tcacgttgaa
      781 aaaagatttc tcaaataaat aaaaaccatc cgtaaaatca tcaatacgat tttacggatg
      841 gtttttgata acttctttga ggataggctc ttgctattta agaatgtaag aaacattatt
      901 gttacatagt atgagagttt aatcctctgt tatttatgct atttaaatgc ttgattcaca
      961 tcttcgatca tcagtttcat tgattctaaa ccgcccccgc ttaaatacca gacatctggt
     1021 tcaagggaga tgatctgctg atttttacca gcattcgttt gtgctaccag ttcattggca
     1081 gaaatatcat ctttgctgtc gtctccacca atagcttttg ttctgtctac tacaaataag
     1141 atatccggat ttttttccag cacatattca taagaaacac tttgaccgtg agtggatgct
     1201 tcgatttgat catctgcttg ttcaaagcca aataaatcat ggatgaaacc gaatctagaa
     1261 ccagatccgt aggctgataa ttgcccttca ttcacaagtg tgactagtgc ttttttgtca
     1321 gtagcttctg ctttcttttt cgtctgatcg attgcactag ataattcctc taattgagtt
     1381 ttagcttctt cttgcttacc aaagatctcc cctaaagcag tgacattttg ctggaaagat
     1441 tcccatggat ttttcgcatc taaagaaagg aaaatggtcg gagcaatttg acttagatct
     1501 ttttgaaaat ctctttgtcg gccagaaatg atgattagat ctggctgaag ctgattaatt
     1561 ttttctaagt ctggttcttt tattccacct gctgattcta cttttttata ggatgaaaga
     1621 tactcaggaa ggctgcttgt agctgctcca actaccttat ctccaacacc aagagcatcc
     1681 atcgtatcta aagaaccatt gtcgaatacg acgacttttt ctggattatt tggcacctcg
     1741 atttggtctc cattactatc tgttatcgtc aatgtagttt cagtttgtgt gctgcttttt
     1801 tttgtgtctg cctgactggt gttgttgcta tttccacatg ctgctaaacc aaatgatgct
     1861 agaatcaaga tacctactac taattttttc atctcttctc tcctaatgtt agttttagaa
     1921 ataaagacag aatctgcggc cttcaatctc acaaatccgt atgtccattt cgtataattc
     1981 atccaataca tcttttttta tcatttcttg ggtcgttcct tgcgtaaata actgtccatc
     2041 tttcattgca acaatttcgt ctgcataact tgcagcaaag ttgatatcat gaagaacaat
     2101 aacaattgtt ttcccgattt cttgtactaa attttttaaa atctgcatga tttgaaccgc
     2161 aaaattcata tctagattgt tcaaaggttc atccagtaaa atataatccg tgtcttgcgc
     2221 tagcaccatt gcaatatacg cacgttggag ttgaccgcct gaaagagtgt caatcgattg
     2281 atccgctatt tcttctaacc caaggtgttc cagtgattcc tgaacgattt cctcgtcttt
     2341 tgtcgtcaat tttcctttcg tataaggaaa tcgtccaaat tctactaatt ctttcacggt
     2401 gatttggaca tttatttcat tcgattgtcg taggatcgat aattttttgg ctaattcatt
     2461 ggatttccaa gcctttactt cgttatggtc taaatagatt tcacctgtat cttttggtat
     2521 caaacgactg atcatcgaca acaccgttga ctttccagca ccatttggac cgataaaagc
     2581 agtcaacttc ttttcaggaa tcgttaactg tacatcttta accactgatt ttccatggta
     2641 cagtttattg accttttgta aatcaatcat aggatactct tcctttcgcg aatcaatttt
     2701 gtgacaaaat aaattcctcc gacaaattca acgacgacac tcactgttgt attccagccg
     2761 aaaatctgct ctactgcgag ctgtcctcct gtcagaaaca gcatagctac cagaaagctt
     2821 ccgataaata gctgaccatg acgataagta gaaaagacac gataactgat atttgcgaca
     2881 ataaacccta aaaaagtaac tggacccaca agagccgttg atgtccctgt caaaagactg
     2941 acacaacata aaatcaaaaa ttcaaaacga tctgtgtcta tcccgaggtt tactgcttgt
     3001 tcatttccga gatggaaaac gtctagcaca tgtcgatatc gaaataacag gtaccagcag
     3061 ccgataatga tcaaagaagc aggcaaaaga tactgactct ggatattgcc aaagcttgca
     3121 aataatctgc cttgaagcaa atcatattca ttcggatcca tgatgacttg taaaaaggta
     3181 ctgacactac caaaaagcgt acctaaaatc atccctgaca tcagtaataa aaaaagattc
     3241 gctctcattt ttttcaaaag aaagaaaaat aacaatagac tcagcccgac catcagccca
     3301 acgttcaata agaaaaaaac tgtacctgtc tgctgaagca tcgtcactcc tccgataaag
     3361 aagaaaagga ccgtttggac aaggacatat aacgactcaa gtcccaatac tgaaggagtc
     3421 aaaaactggt tatgcgtaat tgtctgaaag ctgattgtgg cactgctgac agctagcgag
     3481 acaaggataa atgcaaaaat tttcttactc ctcaatgcac atgcaaaatg ccagtttcca
     3541 tatgtatgga tcgtcaagta cattcctgta acaaataaaa caagaacaag cataccaata
     3601 aaaatagatg gctttttttt aagcatatcg tcgtcccctt atcagaagat aaaggaagat
     3661 agccccaccg atgattccaa cgatcacact gactggtatt tcataaggaa aaatcaacac
     3721 gcgagcgaca agatcgcaaa atactaagaa caaactacct gctaaagcag ttacgccaat
     3781 tgttttgtgc acttgatctc catatattct agagacaaga ttcggaatga tcactccgat
     3841 aaaaggaatg cttcctacca tgatcaacat gactgcacta gccaatgata ctaagccaat
     3901 gcctaaaaac tgcatcttct ggtaatctat tcccagattt actgcaaacg attcgcccag
     3961 accaatgatc gtaaatcggt aagcaaatag ccaaaccaag agcaacaatg gaacaacagc
     4021 atacaataac tcataatttc ctcgtatcac agtcgcaaaa ttcccttgaa gccatgaagt
     4081 catattttgt accaactgga attgataagc aaaaaaagta gcaactgaac caatgatatt
     4141 ccctaacatg actccaatca acggaagcat caattgattt ttctggagga acaatcgcgt
     4201 aagggataaa aaaagcaatg ttcctgcaaa agcaaagcaa aatgctgtca aggatcggac
     4261 aatcaaggat gcaccaggta ggaaaatcat agccactaaa atacctaaac gcgcactatc
     4321 catcgtgccc gcagtcgttg gtgaaacaaa tttattttgg gtcaaatgtt gcatgatcaa
     4381 cccacaaata ctactggtcg ctcccacaag gatcaaacaa atcgttcgtg gcaaacgagt
     4441 cactagcaag atttgtaagc tttgcccatc taactggagc agttctgaca attgtacatt
     4501 cgatactccg atgaacaatg aaatactact taaaataact aataatgcta ctagtcgtcc
     4561 gtagcgcatc caatccacct ttctaattga gaatgatttt cattcttatt ttaagtgaat
     4621 cgttgaacaa aagcaaccgc tttttcatat gttttaggta ttattgggaa tatgctataa
     4681 tcgtaagaaa gacaaagaga agaaggaggg tattttttat ggcacaagat gatgtaaaaa
     4741 gccatttgga taagggactt tatggtacac ctttagtgaa ccctgaagaa cagcataaat
     4801 atttagggac attcagggaa cgctgctatc ttagtatgac tgttgctgaa atgatccaac
     4861 caaaaaacaa agaaaacttt ttaaaagaac tcaaaaaaca tccagatgct tccatactct
     4921 tgaatggggc tctacccctt tctgtccaaa accaatatat ccagctagca acgaaaaaca
     4981 atagccgttt cactgtagtc aacgattacg tcactgcaaa tcctgaggaa ttcggattgc
     5041 tcttaacagc aaaagaagca gtaaatgaac ctgtgattga tatcgaaaaa aaatacccag
     5101 aagaaaactc ttcgccagct gaaaaagaaa aaccaaaaaa gaaaaaaagc ttctgggatc
     5161 gtttatttta aaacgaaaaa acacataaat ttttacatgc aaaaaaagct tttgatcaga
     5221 ttgacatcaa ataggacaat ctgtcaaaag ctttttcttt taaaatactt caggatataa
     5281 caatttcaat acattcatcg tcaatcttct gccttttcct gcaaaaggat aaacatgcat
     5341 atgcatggct ggattgaaat ttgcttcgat gatcccatat gcttttttat cagttgtagg
     5401 atcgatttct ttatctggta taatcaaatc gataccacta atcttagctc ctagtgcttc
     5461 taccgcagaa acggcgattt ttttgtaagc ttcagaaaat tcatcagtca tatcgatcga
     5521 atcaccacct gtactgatat ttgagttctc ccttagataa acaatttgat tggcttgggg
     5581 aactgattcg atcgtcaacc cttgttcttt caacatcaat tgttctaatt cacctaattg
     5641 gatcaattct aaaggtgcac gatgattcgt tccacgcaaa gggtcactat ttttttcttc
     5701 aacaagttct ttcactgttc ggatactgtc accaataaca ttagcaggga ctcgcaacat
     5761 gatggcttgg acttctccgt cgatcacaaa gaaacgatat tccgttcccg gcataaattc
     5821 ttctactaaa accgaactgt cttcacgaaa agcaatggct aaacctgctt gataatcttc
     5881 cagactagct ccttccttga aaatcgtgat acctaacccg taattggttg atttaggttt
     5941 gatcacaaat gcctgctcgg caaatctcgg ataagctttt accgcttctt ccatggatga
     6001 aaattctgcg cctcctggta cccggaaccc agcttctttt aagacttttt tcgttactgt
     6061 tttgttttcc atgataagtg ggacgatata gctgtcctta cttgtcatgt ttgcattttt
     6121 cacatattct acatggtctt ggaattgcag acgcaaaaat tgatccgact catctaatac
     6181 tttgacttgg acaccttttt ggatggcatc gaacataaat atctgagtag aaagttccat
     6241 ttcgcgaaaa ccggccaact gatagggacg ttcatggctt tctttataat attgatgtcc
     6301 caatgccaca gcaacttctt gctgactcga tagctgtgct tcttgataga gtttcgctgc
     6361 taacgtttgt tcaggatgat ccatttgagt caatgcttcc tctactaaat aaagagaatc
     6421 caaccaatcc aattcaacca gcatttgctt cattccttca agaatttctc taccttcttg
     6481 atgaaattct gtttgatctg atggttgttc tagtgctact ttgttatttc gagtcgtacc
     6541 ttctgctacc cattggtcac agtcttcttt ttcttctgtc cataacatgt acatcaggaa
     6601 taattgtaag aaccgaactt gctctggatt gatacccaat cttgcataag gattcaaatc
     6661 gatattccgc aattcaatat aacgaacacc tgttgttgct aaatctgcga ctttctttcc
     6721 gcctcggaaa cgaagcggtg tataaaattc tttctcctcc gaaagtttcc cttcttcgat
     6781 cattcgctcg atatctgcta aatactgttt catagaagca taggaaactt tcacatttgg
     6841 atgattcgta tagcccagcg cactgttgcg aatactgcga acaggttctt gcggatgtga
     6901 ttcatctaaa aagtagttct tctcactcat tggtgaagca ccgaataaat aagtgatcat
     6961 ccagcgatag cgcataaagt ttctcgctgt tttcaaataa agttctgttt tgaattcgga
     7021 aaaatcttga aattcttcct gatggctgaa caatgttctt agcaactcat cgccaaattc
     7081 aaaattgaaa tgaataccgc ttaccatctg ttttcgcttc ccgtattttt ttgctaaata
     7141 acgtcgatac aagacatctt cgaaattttt taattttgca ataatgatct cttcgtcttt
     7201 ttccggtaag gcaggtggca tacttaatgg ccagatcatc tcttctttcg gtatagaacg
     7261 agcagtcaca tcataaacag cagcgagata ctgaaataat tcgggaatag aatcggtaac
     7321 aggggtgatc atctctgttt gtgtttcgct aaaatctgtt tggatatagg ggtgatagga
     7381 tcgatcgcca aagattgctg gatgatcggt ttttgctaga tttcctgcaa gatcaacccg
     7441 ctgtccttcc cgttctatac caaatcgagc ttggtcgata aaagggcgcg cattgacatg
     7501 caataataat tgcttaaaat tcatcattaa gtgacctcca attcatatga atctaattat
     7561 aatcaaaaag tctaagaaaa agaacgaaat tgtttgagaa ccgcttacaa ataagtataa
     7621 aaatgaaggc atcaaaaaca cagctatgtt aaaaacaatc attcaaacaa ggagctgcaa
     7681 cttttgtcac agctcctgct ttttagacat gggttattct tcttttagta atcctttgaa
     7741 caatccaacg acaaaatcat ttggatcaaa cggctccaag tcatcgattc cttcaccaag
     7801 accgaccaat ttcactggta aatgcaattc attacggata gcaagtacga taccgccctt
     7861 tgctgtacca tctaattttg tcaaaacaag accggtcaca tctgttgttt ctttgaattg
     7921 ttttgcttga accatcgcgt tttgaccagt ggttgcatct aatacaagaa gtgtttcttg
     7981 gggtgcttct ggtaattccc gctggattac tcgttttatt ttttctaatt cgttcatcaa
     8041 gttcacttta ttttgcagtc ggcctgcagt atcaactagt aaaacatctg cctgctctgt
     8101 ctttgctctt tccaaagcat cgaatacgac agctgctgga tcgcctcctg cattgccacg
     8161 aacaacttca acaccagcgc gttcgcccca gacaactaac tggtcgattg ctccagcgcg
     8221 gaaggtatct gctgctgcca tcaatacttt tttaccttcg tttttgaact gatgggcaag
     8281 tttaccaata cttgttgttt tccctacacc attaacaccg acaaacaaaa taactgttag
     8341 tccgtcttct tggatattta gttcatttac ttcattgatt ccttccgctt catagagatc
     8401 cacaagtttc tcgatgatcg cattttggac ttctgccgat tttttcgcat ttctgagttt
     8461 cacttcttga cgtaatgctt cggtgattcg tatcgctgtt tcaaaaccta catctgcacc
     8521 gataagtgtt tcttctaatt cttcaaaaaa gtcttcatcg actgttcgaa aattggcaaa
     8581 caactcattc atccgttcac caaatgtttt acgcgtcttt tctaaaccct tgtcgtactt
     8641 ctcctgtact tcaacttctt ctgttttctc ttcgccagta aaagcacgct tgattttatc
     8701 aaaaaatccc atttgactcc ccctactcta atacaaattt ttggatcact ttggctacgc
     8761 cattttcttc attgctggct gtcacgaaat ctgctgcttc tttgacgaaa ggcacagcat
     8821 tctccatcgc tacgcctagt ccagcatatt ggatcatcgg taaatcattt tcctcatcac
     8881 cgatcgccat gatttcttct tgtttcaaac caagatcttt tgccaataaa gaaataccat
     8941 aagcttttgt aacacctttt ggcataaatt ccaaaagcat acttcttgtt ttgatgatct
     9001 catacatttc atggaattct gctggtattt ctttgatttt aggatctaat tcttcttggt
     9061 cataagcgat tactgcctta ttaagcacaa attcatctgt aatatcgctt atatccgctg
     9121 gttggaattg cagcagctta ttcaacacat tgtacaagga ttgcttcctt ggggcactag
     9181 gcagctgtaa aaccacattg tcagacaaaa catctagtgg cagatccaat tgctgagcca
     9241 gtttgtataa gcgttggata tctgatactt ccatcacttt cttttccatg atttcaccag
     9301 tatcattttt ttgtaccaaa ccgccgttga aagtgatgct gtagtcacct tcatctacca
     9361 gtcctaattc ttgcagataa tgcgccatcg cagctaaggg gcgtcctgta catagaacga
     9421 tcttgacccc ttgctgtttt gcttgtgcta atgcttgttt attctcatct gaaatcagct
     9481 tttgttcatt taataatgta ccgtccaaat cgattgctac taatttgatc attttcgttc
     9541 ctacactttc gtttcgttga ttttcgcctc ttcattgata tcttccatcc gtacagaaac
     9601 gatttttgat acaccggatt cctgcatcgt cactccgtac agtacatctg ctgcgaccat
     9661 cgttcctttg cggtgtgtga ctacgataaa ttgtgtgtca tcttggaaat cgctcaagta
     9721 acgaccaaaa cgtttcacgt tggcttcatc taacgctgct tctacctcat ctaagataca
     9781 aaacggaaca ggacagacac ggataatgga aaagagcaac gcgatggctg taagtgctcg
     9841 ttctcctcca gaaagcaaac ttagactttg cagttttttg ccaggaggtt gtacttcaat
     9901 ctcaatacct gttttcagca aatcagttgg ttccgtcagt actaattcag ctttaccgcc
     9961 gccaaacata tttggaaaga caattttgaa ttcttgacgg atagcttcaa atacttcttt
    10021 gaatcttgtc cgcacttcat catccatctc atccatcgtt tcaaacaatt ggtttttcgc
    10081 tgctaacaga tcatctcgct gtgttgctaa gaatgtatgt ctttcactga cttgttcgta
    10141 ttgttcgatt gaatttaagt ttactggtcc cagtttttca atctgttctt ttaaggatga
    10201 aacctttgtt ctactgtctt cgatatcggt tgtttcttga tagtcttggg aagctttctc
    10261 aaatgtcagc tgatattctg actgcagata ggtcaaatga ttatctaaaa tcatttcagc
    10321 tcggtctttt tggacttcta atttggattg ttccgacagt cgtgcttttt gctcacgatt
    10381 acgctctgcc aagactgctt caagcttatc gatctccttt tgccttctat ctcgctgctc
    10441 tttggctttt gccaactcgg cttttaatgt ttctcgctga gcagataatt cgttaatttg
    10501 tttttccaaa ctttcttctg tgacttcatg atcagaaaag tctgctgtca atgtcgccaa
    10561 ctgcttttct atcgcttctt gacgttcggt tgcctctgct ttttggactc gggcaccgcg
    10621 gagctggatc tgcagatgat taaactgctc tttcaagaca gcaagatctg cttgttcttg
    10681 tgctttttga ctttgtactt gtgctcttct ggcttccatc tggtcgcttt cttgacttaa
    10741 cgatttgatt tcttcgtcga ttttttgtcg ttgcgattca agatctgttt gtttgtcttt
    10801 caattcgttc gtttgctttt cgtaatcttc gataaactgc tgcagttcac gtgtctcaaa
    10861 agttgagatt tgcttttctt tttcaaaacg ttcaagttca tttatgatgt tttgtagctg
    10921 atttgtcgct tcttgttctt caaaacgtaa ttgttcgcct cgtgtacgca aaacttcttg
    10981 ttcttcggct aaacgagctg tttcttgttg cagctcctga actttttttt cttgtgcttg
    11041 gagttgttta tctgcttctt caaattcact agtcaactgt ttcaattctt gattttgtac
    11101 aaacaaactg ccttggtttc ctcgtttatt cgctccacct gtcatggatc ctccggcatt
    11161 catcacatca ccttctaagg aaaccactcg atattgatag cggatcgttt gcgcgattgc
    11221 attggcacta gttaagtctt tggctaaaag gatcgttcct agcaggtttt gaaccacagt
    11281 ttggatttga tcgggataac tgacttgttc actggcaatc cctataaacc cttctacagc
    11341 tgctgcttgt gtcaatatat gagccggtaa ttgtcttggt ttgattgtcg tcagtggaag
    11401 gaatgttgct cttcctcctc tttgctgttt caaatacgtg atggcttgcc gagcatcttt
    11461 ttcattttca acgatgacat gctgggctgc cccacctaaa gctgcttcga tagctaacgt
    11521 gaaatctgca ggaacatcga tcagttcagc cactgctccg acaatacccg aaagctgctg
    11581 tttatgctgc aagaccaatc gaaccccttg gtaaaaacca aaatagtttt cttggatttc
    11641 ttgaaggctt ttcttacgag cacgtagctg ctggacttga ttcattaatt gatacatttt
    11701 aggctgttct tttgctaatg cttcttggaa ctttttcccg ttttgttgga tcatttctaa
    11761 cttctctttt gtttcagtta aagatgattt tagctgcgcc tgcttttgtg tcaattcttc
    11821 tttttgtaag atcaatcggt ctactgatgc ttctacttcc gattgcttgg ctaacgtctg
    11881 cttgcttttt gctgtttctt gtatatattg acgttctaga tacttcagtt cgtttcctac
    11941 agctgcttgt tcttgcatca aatcaacata ttgatctctt aattctgcca ataattcttt
    12001 cgttgatttt tggtattttt ctaattcttg ctgtgttttt atgagatttg cttcagcttt
    12061 ttggatctct gtttcttttt ctgctgctgc tttcatcaag ctttcttgaa gcttttcaaa
    12121 atgtttaacc tttttttgtg cttctgctaa tgaagtttga tattcttgcg agcttttttg
    12181 tgtatgcttt gtccgctcct gcaaaacatc tttttgtcct tctgtctgct tcagtttttc
    12241 cgaaagatct aataatactt gctgattttt ttcgatcaga cgatcggctt gcgcattttc
    12301 tttacgctgc ttcgccaaaa tggattcttg ttcttggatc gactcagata gtttgcctaa
    12361 ttctagattg aatttcgcaa gctgtgcttg tttgttgtcc caatcttttt ttgctgtctt
    12421 gatttcagca accatcaaag agacatcggt ttgagtcagt gtttctttta gccgtaaaaa
    12481 ctcctttgct gcctcactct gggcggccaa aggagttaac tgttcttcca attcgtggat
    12541 gatgtcttgg acgcggctga ggttgtcttc tgtttcaaaa agcttctgtt ctgctttctt
    12601 tttccgctgc ttgtatttca agacaccagc tgcttcttca aagatcccac gacggtcttc
    12661 cggtttacta ctgaaaatcg cttctacttt tccttgagaa atgattgaga acgattcttt
    12721 tcctaatcca gaatctaaga acaattcttg gatatctttt aatctgcagc tttgtttatt
    12781 gatgaaaaac tcgctttccc cagtccgtcg ataacggcgg gttacactga tctcattgta
    12841 ctccaatggc agataatgat cactattgtc taaaatcact gtgacttctg caatattcag
    12901 ctgcttacgg gtatcggaac cggcaaagat gatatccggc atttttccgc ctcgcagact
    12961 tttagcagat tgttctccta atacccagcg gatagcctct gtaatattgc tttttccgct
    13021 gccatttggt ccaaccactg ctgtgacgct attttcaaaa tcaatgactg tcttgtctgc
    13081 aaatgattta aaaccggcta tttcaattcg tttcaaatac aaagctttcc ctcacttact
    13141 tttacagtgc tgccaatgca ttttccgccg cagcttgttc tgctaatttt ttcgatttcc
    13201 cttgtccagt gccaatcaat tgatcatcta cataaacttc gatccaaaat actcgttcat
    13261 gagctggacc ttcttcattg atcaatcggt attcgattga tacatcacct gatttttgca
    13321 agacttcttg tagttttgtt ttatgatcca tctcatgtga aaaagcacct gctttgactt
    13381 taggaaaaat cactttttcg atgaatgcat gggtcgtgtc gaatccctgg tcaagatata
    13441 acgcgcctaa aaaagcttcg aataggtcgc aaagcaatgc tggtcgcgta cgtcctcctg
    13501 aattctcttc tcccttgccc aacaaaacat attgatcaaa gtgacattct ttggcaaatt
    13561 tcgacaagct gtcttcgcga acgatcgcag ctcgtgtttt tgtcaatttt ccttctggca
    13621 aatgaggata taaacggaat aaatactgag aaaccatcag ttctaaaacg gcatctccta
    13681 aaaattcaag tcgctcatta tccgataatt gcagattgcg atgctcattc acataggatg
    13741 aatgggtaaa agcttgttca agcaaattta gatcgtgaaa aacgatgtta tagttttctt
    13801 ttagttcttt tgtcagctgg ttgtccattt tccacattcc ttttatggat attttttcga
    13861 tacttcctta ttatactggt tttttgtcaa atttgaaatg gttcggtcaa aaggtaagta
    13921 aagctttaat aatcctagtg attgacgaga agttgtgaaa aattcgttta gtttcgagta
    13981 tgaagaaaaa attttgtgaa aatggctttc catatttttg cataaatttg acttaatacc
    14041 gagaaactgg actttgaaca ccgtttattg gagaagtcat gaaatcagcg ccttgacctg
    14101 aatattagag aaataaacag aaaaatatca aaaaaccgaa aaaggacatc gccctttttc
    14161 ggtcattttt agtgattatg aacgtgaatc tgctgtaaca gaaacatttg cccatgggtt
    14221 tggtacatca taagaccaat cccagtcttt gacacggtcg ttgactggca atacttcatt
    14281 acggaacaac gttggaatcg caaatgcttc ttcaaatgca tattgttgcc atttttgata
    14341 tgcttctttt tggtactctt catcaaatgc tttgcttgaa ctgatatcgt tgatcaattt
    14401 tgtattttct tctgattcat aacgagtgta gttgaacgca gcatttggtc cccatagtcc
    14461 acttggattt ggatcagttc ctgtattcca agcagcttgg taaacatcaa tttcaggatc
    14521 atcgttttct aatttatcat agaaagaatt gaattcgatc agacgacctg ttgtgtattg
    14581 gacatccaaa ccaatttctt tccattgttg gatatagtaa tctgccaatg gttgcgctgt
    14641 ttccccaccg gacatcgaag caaagttgat tgttagttta tttccatctt tgtcttctct
    14701 taaaccatcg tcatccacat ctttgtagcc agcatcatct aatagttttt tcgctttgtc
    14761 tagatcttgt gtgaatcctt tcatctcatc atcatggtat gactggaata ctggaggaat
    14821 caatgaagtc gcattgcttc tcaaacctgc atagaaacgt tcacctactg cattattatc
    14881 taatgcatag cccattgctt gacgtaatga tttatcagcc atttttgaat ctggattgta
    14941 ttccacactc tttgtatctg cattccattt acctaacttg aatcctagat aagtataaga
    15001 agtttgttca cggcctaagt ttgtatagcc agcaatttct ttataggtat cgtaagtatc
    15061 tgttggcatt tggaaaatca tatcatataa tttagagttc aatgcttcag ttgcacttgc
    15121 catcggtaca gatttcatcg tgatcttttt caatttaggc gtaccattcc agtagtgctc
    15181 gtttggcaag tattctactg attctcctgc aacgatatta ctcatgtaat aagggccaaa
    15241 agtcactgga tttttacgta cttgatcgct tgattccata tctttgattg ctacaccttc
    15301 taacgtatgt tttggtgctg catatgacca gataccacca cccgattgaa gcatgcttgg
    15361 gtttacttct ttatattcga tttcaactgt tttgtcgtct atttttttga ttccggagat
    15421 tgtgtcggca cttccatcat gatattcttc catacctacg atgttacgga aagtgtcgtc
    15481 ataacggata ccagtgtaat ctttattccc aatgatttcg taagggaaaa tcacatcgtc
    15541 cgccgttaca tcttcaccat ctgaccattt gacgttgtct tttactttga ttgtcgcttt
    15601 tttcgtatct tcatctagat caagcgttgc agctccgtcg tctgtgattc tgaaatcgtc
    15661 atcgtttgca aatagcggtt catgagaagg agccataaaa gctgcatcat atgaatcttg
    15721 gtaaaactcc cactggaaca atcctttaaa ctgactgtcc gttgcgaccg cagcttctaa
    15781 ttcgccgtct tcgattgcat catcattatt ttttgttgca attggaaaag ttaattcctc
    15841 tttgttattg ccgttgcctc cagagttgtt tccagaggta tctgtacctc cgcccccgca
    15901 tgctgcaaga acgacagcag acatcagcgc tgcaaatcca aaaatcgttt tcttcattcc
    15961 tatttccccc ttcgcttcta tactagccta accgttggcg cgcatctgca gaacgcttga
    16021 atgcttgtcc aacataattg atactcaaca tcaaaactaa aataaggatt gatgcgggta
    16081 accagatcca ttgccgattc actaagactt ctccgttact tgcataaccg atcaatgtac
    16141 ctaaactagg tacatttgcc ggcaagccga aacctaagaa agtcaaagtt gtctcgatac
    16201 cgatgtttgc cgcaaagttc atcgtcaaat tagtaatgat taatgaactt agattcggca
    16261 tgatttcgcg gaacatgatt ttaaaatcat tggttcccat tgtttttgag gcactgacat
    16321 aatctcttct tgcttcagat aatgtcttgc ttcggaataa tcgggcttta gctacccaat
    16381 aaaatgcact catgatgaat ataaaggacc agacattgta tctggaaata atcgatacga
    16441 agacgataat gatcagcatg atcggtagaa tcatgataaa atcaacgatc cgcatcaaga
    16501 tgttgtcaat gatccctccg tagtagccag agatgatccc taatccaacc ccgatgatcg
    16561 aagtaaggat cgtgatcgca aatccgatcg taacagagtt tctagcacct atgatcaatt
    16621 gtcctaatac atcacgtccg ccttcatctg ctcccagaat gaacttttgt ccttgtgtcg
    16681 aaactgttcc tggtgcagcg tatttgtcga agatactaac atacatcact ttgtcttgat
    16741 cggtcagcat cgcaccgata aagatcacta ataataaaac cactagcaaa cctaatgaaa
    16801 aaatagcaat tttatctttt ttaaattctc gagcaatcat tcgtatcccc ataggaggaa
    16861 tactttcttg aactgctgta ttgttttttt cactcatcga tccattctcc tttctgattg
    16921 gttttattga atccgaatac gcgggtcaac gatactcatg atgatatctg aaagtaatgt
    16981 ccccactagc gtcgcgattc ctaaaatcaa tactaaggat gtgatcacgg tatagtcccg
    17041 ttggccgatg gaatcaataa atagttttcc gactcccgga taaccaaaaa tcttttcgat
    17101 aactacagaa ccagcaatca acgaagtgat ttcatacccc atttgagaag ccatcggcaa
    17161 cgctgcattt cggaaaatat gtcgactgaa tactttattc gtcggtaccc ctttggaacg
    17221 ggctgttctg acaaaatcga gcgatttcga atcgattact tcacttcgca gatattggat
    17281 tgtgacggca gtcgccaaaa atgcttgggt aattgatggt aacagaatat atttcaaacg
    17341 gtctatccat tgagtgataa atccaccttg caaacctagt gtttgtgatc cacttgttgg
    17401 gaaccagcct aaacgataac caaaaacaaa tagcatgatc aagccgaaga taaataaagg
    17461 aacagcaaaa ctaaagaagt tgtagattac gacaatctta tccaagattg aattttgata
    17521 ccggccagca agcatcccca gaggcacagc aatcaaataa gtaaggacaa ctgtcaagat
    17581 cgatagatat aaagtgttga tgatccgttc gccgatcaag tcagcaacag agcgtttgta
    17641 taagaaactt tgaccaaaat cgccttgcaa agcatttccg atccaacgga agtactggac
    17701 ataccatggg tcattcaagc cagctgcttg tcgcatctgt tcgatcactg ccggatcctg
    17761 atttggattg atcaaaccgg taaatggatc ccccggcatc gcttgggcta ataagaagat
    17821 cagaacactt aaaataataa cttgcgggat catcaataaa acacgtcgca gaatcgtttt
    17881 ccacattagc ttgcacctcc atctttcaac gccactttgt gtgtagttgt gatcgtacgc
    17941 aaatcgtaga ctcgaccagt cgcatcatag tattcttttt ggttttggat atactcttgt
    18001 tctactcttc gtcgattttc tttgtggctt tccctatttt ctacatcgat ttgcggaata
    18061 gctgaaagca gacgtttcgt atagatatgt cgcgggtcat tataaatgtc ttctcttgta
    18121 ccgatttcta caaatcgacc tttgtacata attgcgatat tgtcgcacat gtgttttact
    18181 acacctaaat catgtgaaat gaataaatag ctgaggccgt attcttgctg gattcgtttc
    18241 atgaaattca atacttgcgc ttgaacagat agatccagag cagatacagg ttcatcggct
    18301 acgatcagtt ttggattggt agcaacagca cgggctactc ctagacgttg acgttgtccg
    18361 cctgaaaatt catgaggata tttatatagc gcatcttccg gcatcccaac gatatctagc
    18421 agtcctttta cctttttctt ttcttcttga tcgcttaacc gttcaaaatt tcggatgggt
    18481 tcagcaatga tatccaacac tcgcttttta ggattcaaac tggacattga atcttggaag
    18541 atcatctgca catccttgtt ataacccatt tttttgcggt tgcttcgctt cgttacatct
    18601 tttccttcat acatgatttg tccggctgtt accttttcta atcccacaac ggcttttccc
    18661 gtagtagatt ttccagagcc agactctccc actaaaccat atgtctttcc tttttcgata
    18721 ataaaatcca caccatctac agcatataca tgatccgtta ctcgattgaa gaatccactg
    18781 cgaatagggt aatgaacttt caggtctttg attgtgatta attctgtcat ctacacctct
    18841 cctttttcgt ctctaaagtg gaaatgctta tagcaggagc aacgtaccaa gtggttcggt
    18901 gcgacttcat gaaggatagg ctcttcttca tgatcgcttg ctggtatcca tggaatacgt
    18961 ggtgcaaacc gacaaccttt acgaggcatg tttttcagag aaggaacgac accttcgatc
    19021 acatggagtt ctgcatcatg atttccttct tgtggtatag agttcagtaa agagcgtgta
    19081 taaggatgct taggatgatc aaacagttct tttacacctg ctacttcaac gaactgtccg
    19141 ccatacatca ctgccactcg atcagccatt tctgctacga ctcctaagtc atgagtgatc
    19201 aagatgatcc ctgtttttgt ttcttcctga aggtctttca gtaaatcaag aatctgtgct
    19261 tggatggtta catctagggc tgtcgtcggt tcatctgcaa tgacgattgg aggtttgcag
    19321 gcgattgcga ttgcgatgat gacacgttgt ctcataccgc ctgatagttc atggggatat
    19381 tggcgtccaa ctctcgcagg attaggaatc cctacttgtt ctagcaattc tagtgcacga
    19441 gcttgacgct gttctgcatt catctttgtg tgataggaaa gtccttcttt gatctggtct
    19501 tcgattctca tgagaggatt taatgcagac aacggatctt ggaaaatcat tccgatatca
    19561 tttccgcgga ttttattata gagcgtttcg ttcaggtcga ttaaattcag atcgttatat
    19621 aaaatctcac ctgtaatttt tgtgttatta ggatcaagta atcccataat cgtagtagcc
    19681 aaggtacttt tcccgcaccc agattcccca acgattgcta aaatctcgtt tttgtctaaa
    19741 gtaaatgaaa cgtcatctac tgcatcgtaa tagtcatctt tgagtcggaa tcctgtatgt
    19801 aggttctgaa cttctaataa ttggttatcg gacaatcgaa tacccctctc tgcttcctaa
    19861 atggaagttt ttcttaataa aaaaggacat tacacagaat aaagcgtttt ataacaaagg
    19921 tt
//
LOCUS       DADVXO010000048        19912 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.049, whole genome shotgun sequence.
ACCESSION   DADVXO010000048 DADVXO010000000
VERSION     DADVXO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.049"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            21..851
                     /locus_tag="KB950_001405"
     CDS             21..851
                     /locus_tag="KB950_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBB6857630.1"
                     /translation="MIGLAILLSGCSKWIDRGESITAVGSSALQPLVETVAETYQTEN
                     PGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIV
                     NKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGE
                     TAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWT
                     IWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE
                     "
     gene            966..1886
                     /gene="pstC"
                     /locus_tag="KB950_001406"
     CDS             966..1886
                     /gene="pstC"
                     /locus_tag="KB950_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBB6857631.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1886..2770
                     /gene="pstA"
                     /locus_tag="KB950_001407"
     CDS             1886..2770
                     /gene="pstA"
                     /locus_tag="KB950_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBB6857632.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2785..3591
                     /gene="pstB"
                     /locus_tag="KB950_001408"
     CDS             2785..3591
                     /gene="pstB"
                     /locus_tag="KB950_001408"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBB6857633.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3607..4365
                     /locus_tag="KB950_001409"
     CDS             3607..4365
                     /locus_tag="KB950_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBB6857634.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4378..5055
                     /gene="phoU"
                     /locus_tag="KB950_001410"
     CDS             4378..5055
                     /gene="phoU"
                     /locus_tag="KB950_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBB6857635.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5293..5592
                     /locus_tag="KB950_001411"
     CDS             5293..5592
                     /locus_tag="KB950_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857636.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5618..6325
                     /locus_tag="KB950_001412"
     CDS             5618..6325
                     /locus_tag="KB950_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBB6857637.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6322..7089
                     /locus_tag="KB950_001413"
     CDS             6322..7089
                     /locus_tag="KB950_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBB6857638.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7189..8757
                     /gene="liaX"
                     /locus_tag="KB950_001414"
     CDS             7189..8757
                     /gene="liaX"
                     /locus_tag="KB950_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBB6857639.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9054..9374
                     /locus_tag="KB950_001415"
     CDS             9054..9374
                     /locus_tag="KB950_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBB6857640.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9374..9724
                     /locus_tag="KB950_001416"
     CDS             9374..9724
                     /locus_tag="KB950_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBB6857641.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9891..10829
                     /locus_tag="KB950_001417"
     CDS             9891..10829
                     /locus_tag="KB950_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBB6857642.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10844..11674
                     /locus_tag="KB950_001418"
     CDS             10844..11674
                     /locus_tag="KB950_001418"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBB6857643.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11718..12743
                     /locus_tag="KB950_001419"
     CDS             11718..12743
                     /locus_tag="KB950_001419"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBB6857644.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12758..13696
                     /gene="galU"
                     /locus_tag="KB950_001420"
     CDS             12758..13696
                     /gene="galU"
                     /locus_tag="KB950_001420"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBB6857645.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13821..14261
                     /locus_tag="KB950_001421"
     CDS             13821..14261
                     /locus_tag="KB950_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBB6857646.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14264..14866
                     /locus_tag="KB950_001422"
     CDS             14264..14866
                     /locus_tag="KB950_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBB6857647.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14934..16040)
                     /locus_tag="KB950_001423"
     CDS             complement(14934..16040)
                     /locus_tag="KB950_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBB6857648.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16250..17251
                     /gene="ccpA"
                     /locus_tag="KB950_001424"
     CDS             16250..17251
                     /gene="ccpA"
                     /locus_tag="KB950_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBB6857649.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17554..19899)
                     /locus_tag="KB950_001425"
     CDS             complement(17554..19899)
                     /locus_tag="KB950_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBB6857650.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         6313 a         3538 c         4256 g         5805 t
ORIGIN      
        1 aacttaaatt attcttacca ataataggat tagccattct tttatcaggc tgttcaaagt
       61 ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag cctttagtag
      121 aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat gtacaagggg
      181 gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc ggaaactctg
      241 atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat catcgagtag
      301 cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac atcagtatgg
      361 aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc ggtggaaaag
      421 atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc acgtttgaaa
      481 aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca agcggaatgg
      541 tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt tcttatgttt
      601 ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa aatgtgacaa
      661 cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat ccaaaaaaac
      721 tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac atcgttggag
      781 aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa ggaaatatcg
      841 ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca tgttattaaa
      901 accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt aagggggagt
      961 catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca aaaatggaac
     1021 agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt gtattatcta
     1081 ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag atcaatgtat
     1141 ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat gggaaaccaa
     1201 tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta tctgctatcg
     1261 ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct cctaaaaacg
     1321 gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct gtcgtttatg
     1381 gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc gggacagggt
     1441 ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact gtcacaacga
     1501 tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca ttagcgcttg
     1561 gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg cccgggattt
     1621 taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct atccaaatgg
     1681 tcatcggtaa tgcagcattg atgccaacag gacttactac tcctgcttct acattgacgt
     1741 ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat aatgtgttat
     1801 ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg atccgtatga
     1861 ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg
     1921 tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac tactttatat
     1981 cttagttcgg ggaataccgc atatttcatg gtccttcttg accgaacctt ccaaagccta
     2041 ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc ttttgatcac
     2101 gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag aatatgctgg
     2161 aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct cgttgccttc
     2221 tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt atggtttctc
     2281 gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga ctagaacagt
     2341 tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg ccttaggttt
     2401 gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag ggattttgac
     2461 tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga tctatacggc
     2521 tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg tttcaagccc
     2581 gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga tcaatacaga
     2641 aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt tgatcattgc
     2701 tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac
     2761 ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac aaatgtcatc
     2821 accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt ctggtatggc
     2881 gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat cactgcactg
     2941 attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg aatgaatgat
     3001 gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga tattaatgcg
     3061 cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca acgtcctaat
     3121 ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca cggcgaaaaa
     3181 aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc cttgtgggac
     3241 caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca acagcaacgt
     3301 ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga cgagccggca
     3361 agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa
     3421 gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat cagcgactat
     3481 actgcctttt tctatctagg aaaagtaatg gaatatgaca aaactaacaa gatatttacc
     3541 agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta agacggaggc
     3601 ctcaacatga caaaagaaat tatttcatct aaagatcttc atctgtatta tggaaagaaa
     3661 gaagcactaa aagggatcaa tctaagtttc aatcaagggg aaatcactgc gatgatcggt
     3721 ccttctggtt gcgggaaatc cacttatctg cgttctttga accggatgaa tgacctgatt
     3781 ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta tggtccaaaa
     3841 acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc aaacccattt
     3901 cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga aaaagataaa
     3961 aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg ggatgatgtg
     4021 aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca gcgtgtatgt
     4081 atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc aacaagtgcg
     4141 cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag agagcggtat
     4201 acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga taagacggca
     4261 ttctttttag atggtcactt gatcgaatac aacgatacga agaagatatt catgaatcca
     4321 gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg aaacggaatg
     4381 ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta cgaaatgggg
     4441 atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca cgataaaatc
     4501 ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac acgcttggag
     4561 aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt gcgtatgatc
     4621 attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc ggtgtctgtt
     4681 gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga aaaagagatc
     4741 tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc ttatgtcaaa
     4801 acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa tgactatttc
     4861 aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac agtcatcagt
     4921 ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta tgtgacaaac
     4981 atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa tacgaaccac
     5041 aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa gaggttgtga
     5101 aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca aacaaaaaat
     5161 agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct tttgaacacc
     5221 gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat catttcatag
     5281 aaaggataaa gaatgcaaaa aaaaattagc atcatcacta tattttttat ttgtgtgctt
     5341 acttcatggg ttattcagca tcaagctgct ttatatgaag tacctgtcat cgagatcgaa
     5401 aaagtacgaa taaaagagga tcatcaattg attacaggaa gactgatgaa cacggatcaa
     5461 aaaggagaat cgatttattt atcggttcct tatcaaaaaa atcaaataga aacagcaaat
     5521 ataaaagcga gacaaatgta tctagtccaa atgcatggag atgaatggca gctagtagca
     5581 aaaaaaaaat gatggctggc ttttcttctt cacaagtatc tccattggcg ccatgttgct
     5641 tattggcgga aaacagggtc tatttgcttt aggaagtctc atcatcaata tcgttttact
     5701 tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa atatatctat
     5761 cctgttcgtc gttataggga tgattattag catattggct ttagatggat ggaatagaag
     5821 cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt ttatttgtct
     5881 tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga attatctgac
     5941 acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg gaggaacact
     6001 cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac aaccagaaat
     6061 aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga ttggctcgat
     6121 gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc ttttatattt
     6181 aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg aatttttacg
     6241 cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc tcttttttct
     6301 gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat tttagcaatc
     6361 agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt attcatcaat
     6421 ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat actggcattg
     6481 tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa cggttataat
     6541 ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt cctactattg
     6601 cttcctatca ctgatttttt agcgatccaa ggttttacat cggtagaact agaagaatta
     6661 tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc gttgcttctg
     6721 attagtctta gtggtgctgt tttagatgct agtgtagcaa tttctagtgg gacattcgaa
     6781 gtatatcagg caaatccgca attatctttt taccaattgc gccattctag tttcgctatt
     6841 gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat gggaagttct
     6901 ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt gatcaatgaa
     6961 aaatcatttg ttctagaata tacgatggct attcttacca cattgtctgc actacttgtt
     7021 cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga acaactcaag
     7081 atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt gcataatatg
     7141 gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat gaaagaaaga
     7201 gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg cttagattta
     7261 ttagaaagca tggcaactga aaaagacgaa aagcaaataa aaaaagaagc agacaaggta
     7321 actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact tgaaaacggc
     7381 gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaaggcaaga tcaagaaaac
     7441 ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc gcgtctagat
     7501 gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca ggaagcattg
     7561 atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt agcacagcgt
     7621 cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact gttagaagaa
     7681 caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc tgagaaaaaa
     7741 gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc aacagataca
     7801 ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa attcttgaag
     7861 aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa agatgtcagc
     7921 ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta tgaagctcct
     7981 gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa aacgtgggac
     8041 agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg tgctgaacca
     8101 tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat ctccttccaa
     8161 attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg agtatacgac
     8221 cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga agctaaagat
     8281 atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc aacgatgctg
     8341 gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt agattctatt
     8401 atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct ttctgtgtct
     8461 ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa agtagaagca
     8521 agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt ggaaggacat
     8581 gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt agttcgtgag
     8641 aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcacg
     8701 caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga taaataaaga
     8761 ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa atagcttccc
     8821 ctattttaca aaattgtgcc ttattgccga taggctatct cttgaacacc gtttaccaag
     8881 agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa aaagctcgtc
     8941 acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac cgattaagaa
     9001 gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga gacatgatga
     9061 aaaaaaagtt gacaaaatcc aataaaaatg ttgttttgac agggacttta gcaggaatag
     9121 gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc ctgagcttag
     9181 ttgcttttgg aagtccaatc attttataca ttttattagc tttgattatc ccatctgcgc
     9241 gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac aattatcgtg
     9301 aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat
     9361 ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa cattcatatc
     9421 attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg ccgttcttgc
     9481 cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga tactgtccct
     9541 gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag cgattcttaa
     9601 aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt
     9661 gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agtgaacata acagtagaaa
     9721 ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc aggaatgcgt
     9781 tttctattac attggtaatt aaataaaaac gtttttttta attggtagta cttttggcta
     9841 gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga atgtcagaag
     9901 ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga
     9961 aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta gagttgactg
    10021 gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa gaaatcagtt
    10081 ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta tgtagtgaag
    10141 atatgcccgc atttattatt tcaagaggat taccggctcc agaagaaatg atccaggcag
    10201 caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt ttattaggag
    10261 aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac ggtgttttag
    10321 tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga aaaagtgaaa
    10381 cagcgttgga gttgatcaaa cgcggtcatc gattgatcgc agacgatcga gtagatgttt
    10441 atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa catctaattg
    10501 aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg
    10561 gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat aagaaatacg
    10621 accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt ccacaaataa
    10681 aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt
    10741 tccgtgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct
    10801 tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag cacaaatcaa
    10861 tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga tcatcgtatc
    10921 aggtatcatt atcgccatgt ggcttagcag cagagaggct gttcgggtag gattaaaagc
    10981 agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg gtgctcgctt
    11041 atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa tattcaatat
    11101 ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca cgctgtattt
    11161 ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg caccaagtgt
    11221 attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag catatggacc
    11281 agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca ttgataatat
    11341 gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt ggagtttgat
    11401 tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa aaggcgaagt
    11461 ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag gattacgtac
    11521 agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg ctgtgttatt
    11581 tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac cggattatga
    11641 ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa aatgaaaaaa
    11701 atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc tcttggggta
    11761 ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg
    11821 cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta ccagatcatt
    11881 atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa gacgcagatg
    11941 cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta
    12001 aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag ggaacacata
    12061 aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa gctgttgttg
    12121 ttctttctgg accaagccat gcagaagaag tagcgaaaca tgacatcact acgatcacag
    12181 ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg aataattatt
    12241 ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct ttgaaaaata
    12301 ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat gcaaaggcag
    12361 caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg ggtgccaacc
    12421 cgctgacctt tatcggatta agcggagtag gcgatctagt cgttacttgc acaagtgttc
    12481 attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta gaagaggtgc
    12541 tagccaatat gggaatggtc gttgaaggtg tgaatactac tcaagcagcg atggaactag
    12601 cccaaataat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac
    12661 aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatgggaaa aaagaaaatg
    12721 aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagtcaaaa aagcagttat
    12781 tccagcggca ggattaggaa caagattttt accagcaaca aaagctatgg caaaggaaat
    12841 gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg
    12901 aatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag atcactttga
    12961 ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat tattgaaatt
    13021 agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc caaagggttt
    13081 aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccgtttg tcgttatgct
    13141 aggcgatgat atcatggaag atgaagtacc attgacacag caattaatcg atgattacga
    13201 acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa cttcaaaata
    13261 cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca aaaactttgt
    13321 tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac gttacttatt
    13381 gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa atgaaatcca
    13441 actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc gtaaattcac
    13501 aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta ttgaatatgg
    13561 tctaacacat ccagaagtag gtgcaccttt acgtgagtat atcctgtctt taggtaaaaa
    13621 attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag aaatcaaaga
    13681 agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga ataaaaagta
    13741 gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga
    13801 gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc gcagcaatcg
    13861 cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct aaaactttag
    13921 aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg
    13981 tagatctttt gtctagacaa gtagaaggct tgctggtcaa aagtaatgaa ctgctcttgg
    14041 atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct gatttaagtg
    14101 aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc ggcggaatcg
    14161 gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg atgaaatttt
    14221 ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta aaaaaggtgg
    14281 atttttatta ggtgcagtag tcggaggaac agccgcagca atcgccgcgt tattgttagc
    14341 accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg atttgatgga
    14401 tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa caggtattgc
    14461 aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta gtgtcaaaca
    14521 aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct tgttaaatct
    14581 taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag atatgaaaaa
    14641 acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca tgattgatgt
    14701 aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag aagcaaaacc
    14761 agttgcagat gacacaaaag aaatgatgaa aatggcaaaa gaagatgcga aagaagctaa
    14821 aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc actaaaatca
    14881 caaatttata gaataaaaag gtgggagcgg actagctctc acctttttta ctcttatttt
    14941 tctaaaacta gtaattcttt tggtgtagtc gtgaatggca cacagccatc gcttgtcaca
    15001 tatacacagt cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    15061 gagaagcaca tcccttcttc gattactaag tcatttcctt ctacaaggga agggaattcg
    15121 tggacggttg ttccgatccc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    15181 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tggacggacg
    15241 gcttcttgtg cggcaagctg tgcttcaaga gtgatgttgt atattttttc ttggaagtcg
    15301 gttggttttt gataagcaat tgtacgagta gcatcactgc aatagccatt ccaaacaacg
    15361 ccaagatcga ataacacaag ttcattcggt gaaatcttcg tgtttcctgg tgtaccatga
    15421 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    15481 tgttttttca attggtattc gatttctgca atgatcgctt gttcagtcac gccttctttc
    15541 actgctttaa agccaatctc aaatgcaaca tcggcccatt taccagcttc gatcagtttg
    15601 tctttttcct ctagagtttt gatcagttgt agacgttgga tgatggaagc tagatcagtg
    15661 taagaatccg ttcccgtcaa gatatcagag atagcatcga agcgctcgac agtcaaggcg
    15721 ttcttttcaa tccctacatt gccaggctga ccatagcgag cacgaagttc ttttccgatg
    15781 atctcccaag ggttttcgct gtctaaatag ccacggacat catacgccca actactgttt
    15841 tgagcatctt caacttccaa agcaggagtg aaaaggaaag gatcttgttc aagagggata
    15901 aaaagagcta gtacacgctc atgaggatca ctttcgtatc cggaaaagta agcgatgtgg
    15961 ccagggtcgc tgatataagc caattcgact tggtgttgtg ccatccattg acgtagttca
    16021 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    16081 tgtaccactg atttattaaa agaccaacgt tctaaaaact gaaaaaagta aaaattttca
    16141 tgaaaacaga gataaaacgg ttgcaattct tgagattatt tgatattcta ttttagaaat
    16201 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    16261 aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg
    16321 tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct tggaagttat
    16381 cgatcgcttg gattatcgtc caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac
    16441 aacagttggg gtaatcatcc ctgatgtcag caatatgttc tttgcttctt tagcacgtgg
    16501 tatcgatgat gtggcaacaa tgtacaaata caatattatc ttagcaaatt cagatggtaa
    16561 cgaccaaaaa gaagtcaatg tattgaataa cttactggct aagcaagtgg acggcgtgat
    16621 cttcatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc
    16681 tgttgtatta gctggctcta ttgatccaga tgaacaagta ggtagcgtaa acattgatta
    16741 tgtatcagca acaaaagatg cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc
    16801 ttttgtcagt ggtgcgttga tcgatcctat caatggacaa aatcgtctga aaggctataa
    16861 agaagcatta agcgaaaata acctaccata tagcgaagga ttgatctttg aagcacaata
    16921 caacttcaaa gatggtctat ctctagtcga tcggatccat aatagcggtg caacagctgt
    16981 ctatgtttca gatgatgaat tagccatcgg tattttggat ggtttattag accgtggcgt
    17041 gaaagtaccg gaagaattcg aaatcatcac aagtaacaat tcactactga cagaagtagc
    17101 tcgtcctcgt ttaacaagta tcacacaacc attatacgat ttaggggcgg tatctatgcg
    17161 cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta tcttgcctta
    17221 tggaatcgaa gaaaaaggat ctacaaaata ataaaaggac aaccattcag aggttgtgag
    17281 cctgccgttt aactctgcgt tacaaggagc aatttcaaaa aacagcattt catgtttgat
    17341 gaatattgtg actcgtgatt cggagttggc aggagaacac cgtttattcg ggaaaagggg
    17401 ttatgacaaa attttgttta atcctttttt tttgtcttaa aaaagagaga aagtggttga
    17461 aattacattt atcgtatata aaacaatccg tgtatagatg ctgcattatg ttcaccacca
    17521 tcaaaaaaat aagggactgt gtattctatt aaatcaatca tctttctcat ctgattcttt
    17581 ggttgcttga ttcgctcttg cataagctga agcggtgttc cagtaatctg tatagttttc
    17641 atcggtaccg ccaacgccaa atttaaaggc gcttttcttc ggtccatttt tcgcccatag
    17701 cgattcaact gtttttcctg gtgtttggat ggatttttta ttgacagtaa cttttccagg
    17761 tgtttgacca gtaaatccag atactttttt cttcatcaca tcagatgata attcgaattt
    17821 ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct tgattgatca gattggcaag
    17881 ataattagcc gaacgtatcc ttgctttgct gtccattcca gtattgtcat cgtgccctgc
    17941 ccagctactg atggtaatag acggagtaga gacaattagc catgaatctc ggtattcatt
    18001 ggtagaacca gtttttccga cccaatctgc ttttcctaga tttccgttta agcttgagat
    18061 ggcatctttg aatggtgtag tgatttttgc attgatcaca ctgcgcatca tgtcattcat
    18121 gatcgatgcc gttgcttccg aatagatccg gacagagttg ctttcgtgtt tataaattac
    18181 atttcctgcg ttatctgtga tggaatcgat gatatagcct tcttcataga cgccgccgtt
    18241 tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg acatctactg tacctaaagg
    18301 agcagattca acgccccaat tgtcatttgc cgggtagttc atctttgcca agtagtgctc
    18361 gtaagcatat tgtttcgatc caccggaatt caacacatct tgatataagt gataagccgg
    18421 gatattgttg gaccattcta gtgactcacg gacggtttgg aaggtgttcg atccttcgtt
    18481 agtagcattg acgattttct cgccagcatt ttctccttct tgccaagttg ttgcataatc
    18541 tgatacacgg gattctgatc cgataagccc catgtcgatt gcaggtccat aaacaagaac
    18601 tggtttgatt gatgagccag cttgacgttc tgtatcgaag gcatggttat tttgattctg
    18661 actgtaattc cgtccgccaa cgaatccgta gatgcggcca gttctgttat ccatcaatac
    18721 atttccggtt tctacttgac tgccagcacc atcatctaac atgtatccat aattttctac
    18781 gccattttgc aatgcagcat agacatcttt atcgattgtc gaatggattg tatatccttt
    18841 attttggatc gtttgctgtg ccttttgata ataagcgtca ctgaccgatg aatcagacat
    18901 atcagcgttg tctttttcag ccaattgttg tgcaatgatt cttgttgctt cattcatcac
    18961 agtataatac aagaattctc tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt
    19021 tgtaaggtca taggcttttg cttcttcgta ttgttctttc gtaatttggc cttcacgata
    19081 catactgaat aaaacaaaat cttttctggc aaggccggca gaaagatctt cttttagcgc
    19141 acctgtgttc gtgtatggac tatacgtgat cgggctttgg ggtaatccag cgatataagc
    19201 agcttgcggc agcgtcacat cttttgcatc tacaccaaag attcctcttg cagcttcctg
    19261 gactccagca atgttttgtc ccttattatt acgaccaaat ggtgagacat tcaaatacgt
    19321 agcaatgatc tcatcttttg aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt
    19381 tgctttacgt ttgaatgttg tttcatctgt aaggatctgc tgtttgacta attgctgtgt
    19441 caaagttgat ccaccagaag agccaatacc agtagcttca gatactaatg ctcgtaaaac
    19501 agctttagga acgtaacctt ggtgtttatc gaagtattca tcctctgtgc tgataattgc
    19561 agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc gtgcgcatca gatctgtctg
    19621 gatttttgat atctctgtat tatctgcata aacaatcttt gacgtttctg taatgtttcc
    19681 tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg tcttcgacga gataggcaaa
    19741 atagccagcg cctattccaa tacctagagc tgcgaacatc aaaagaagaa cagtaataaa
    19801 aacagtcaat gattgaaaaa ctctaaaaat aatcttagga acaaaccatt tttctttttt
    19861 tcttctattc tgtttttttc gagtattttt gcgtggcaaa agggtcacct ca
//
LOCUS       DADVXO010000049        19689 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.050, whole genome shotgun sequence.
ACCESSION   DADVXO010000049 DADVXO010000000
VERSION     DADVXO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.050"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            38..1699
                     /locus_tag="KB950_001426"
     CDS             38..1699
                     /locus_tag="KB950_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6857651.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1769..2131)
                     /locus_tag="KB950_001427"
     CDS             complement(1769..2131)
                     /locus_tag="KB950_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBB6857652.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2199..3140
                     /locus_tag="KB950_001428"
     CDS             2199..3140
                     /locus_tag="KB950_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857653.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3141..4184
                     /locus_tag="KB950_001429"
     CDS             3141..4184
                     /locus_tag="KB950_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857654.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4190..5260
                     /locus_tag="KB950_001430"
     CDS             4190..5260
                     /locus_tag="KB950_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857655.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5250..6203
                     /locus_tag="KB950_001431"
     CDS             5250..6203
                     /locus_tag="KB950_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6857656.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6215..7225
                     /locus_tag="KB950_001432"
     CDS             6215..7225
                     /locus_tag="KB950_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6857657.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7510..8331)
                     /locus_tag="KB950_001433"
     CDS             complement(7510..8331)
                     /locus_tag="KB950_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6857658.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8428..8706)
                     /locus_tag="KB950_001434"
     CDS             complement(8428..8706)
                     /locus_tag="KB950_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857659.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8974..10983
                     /gene="metG"
                     /locus_tag="KB950_001435"
     CDS             8974..10983
                     /gene="metG"
                     /locus_tag="KB950_001435"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBB6857660.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11462..12049)
                     /locus_tag="KB950_001436"
     CDS             complement(11462..12049)
                     /locus_tag="KB950_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857661.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12224..13546
                     /locus_tag="KB950_001437"
     CDS             12224..13546
                     /locus_tag="KB950_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6857662.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13701..14477
                     /locus_tag="KB950_001438"
     CDS             13701..14477
                     /locus_tag="KB950_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBB6857663.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14470..15039
                     /gene="rnmV"
                     /locus_tag="KB950_001439"
     CDS             14470..15039
                     /gene="rnmV"
                     /locus_tag="KB950_001439"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBB6857664.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15032..15916
                     /gene="rsmA"
                     /locus_tag="KB950_001440"
     CDS             15032..15916
                     /gene="rsmA"
                     /locus_tag="KB950_001440"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBB6857665.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16367..16996
                     /locus_tag="KB950_001441"
     CDS             16367..16996
                     /locus_tag="KB950_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857666.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17120..18229)
                     /gene="ugpC"
                     /locus_tag="KB950_001442"
     CDS             complement(17120..18229)
                     /gene="ugpC"
                     /locus_tag="KB950_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBB6857667.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18438..18860
                     /gene="mgsA"
                     /locus_tag="KB950_001443"
     CDS             18438..18860
                     /gene="mgsA"
                     /locus_tag="KB950_001443"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBB6857668.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19183..>19689
                     /locus_tag="KB950_001444"
     CDS             19183..>19689
                     /locus_tag="KB950_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBB6857669.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKI"
BASE COUNT         6393 a         3550 c         4039 g         5707 t
ORIGIN      
        1 taattattct gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg
       61 tttggttgcg gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt cagccacaag
      121 cagtagttca gcaaaaagcg gttcagcaga tggaggaggc gtatttaact tagtcgtgcc
      181 tcaagaaatg cctacagctg acttatcagt tgcaacagat acaatcagtt ttacagcatt
      241 gaataacgtt tatgaaggaa tttaccgtct tgatgaagac agcaaaccac agccggcagg
      301 agctagcgag ttggcagaag tcagtgaaga cggattaact tacaaaatca aattacgaga
      361 agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg gattacgttt atggctggca
      421 acgaacagta gacccagcta cagcttctga atatgcttac ctgtatgcgc cagttgaaaa
      481 tgcagaagac attacagctg gaaagaaaga taaatcagaa ctagggatca aagcagttgg
      541 tgactatgaa ttagaaatca aattgacgaa acaaacacca tatttccaat atctgcttgc
      601 tttcccatca tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc
      661 atctgcaagt gataaagctg tttataatgg accattcaca ttagaagatt ttgatggacc
      721 aggaacagat acagaatgga cttataagaa aaatgatcat tactgggaca aagatacagt
      781 aaaactttct gaaatcaaag tcagtgtagt aaaagaatct tctactgcat tgaacttatt
      841 caaagatggc caagcagatg acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa
      901 cgatccagct tatacttctg ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg
      961 tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa gctatttctt attctatcga
     1021 tcgtgaagct ttagtaaaac aagtactagg tgacggatca gttgtctcta caggattgat
     1081 cccatcagat atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag aagctggaaa
     1141 attagtttct tatgacaaag ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt
     1201 gggcgttgat tctttagaat ttgatttgat ggcttctgat gatgattcat cgaaaaaagt
     1261 aatcgaatat gttcaaaact caatccaaga aaacttggat ggcgtaacag tcaaaccaac
     1321 acctgttcca ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg acgcagtact
     1381 aggcggatgg gctgctgact atgcagaccc aagcagcttt actgatttgt ttgtcacagg
     1441 taattcatac aaccgtggtc gctggagcaa tgcagaatat gacgaagcag ttaaagcagc
     1501 ttcatcaaca gacgctggcg atgaacaagc ccgttgggat gatttccaaa aagcaatcaa
     1561 gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt
     1621 gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc gcttcatggg attacaaatg
     1681 gacatacgtt gaagaataat cgttaatacc gtgagcatga gaaaaaacga ggctgcggca
     1741 aatgtcgcaa cctcgttctc atattcactt atcctaacaa agaaaaaagc atgaaaatag
     1801 cagaaagagc aattagtatt ccggcaatga tcagccaaaa gctgtacata ccgtagctgg
     1861 cagacgagag cgtggaaaat tctggttttt tctttcggtt ccttgttcgt gcaagatgga
     1921 ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa ccgccgataa
     1981 tcagaaacgg caatgaccat aagaatagat cattagataa ttgaatgagt gtaaaggaat
     2041 ggttgatgag attttttatt aaaataacca gaatcagtag aaaagctact gcgtagggga
     2101 tagatttttt ttcatttgat tgctccttta tttttggttc attatctaat aatgtagcga
     2161 aacatctgtc agaagtcaaa agctaggggg aaacattagt gaatagttat atcaaatatg
     2221 tattaaaacg tgtctttttc atgatcatca cgctttggtt aattgcaaca atcacctttt
     2281 tcttgatgca attgctgccg ggcacgccat atacgaatca ggaacgtttg agtcctgaaa
     2341 caatcgagat gctaaataaa caagtcggtt tggataaacc agttatcgta caatatggga
     2401 tttatctttc taatttgctt caaggagatt ttggaatttc cttccaattt aaaaaccagc
     2461 cagtcgcaca tttgttagct ggaagaatcg gaccatcctt gcagttaggg ttacaggcaa
     2521 ttatctttgg aacagtccta gggacgattt taggaacgat ctctgccatg aagcaaaata
     2581 catgggcaga tacatcttct acactcgtag cgatattagg tcgttccatt ccaaactttg
     2641 tatttgctgt tttattacaa tatatctttg caatcaaatt acaagttttg ccaatcgcaa
     2701 aatgggatgg ctttgtgtac acaatcttac caacaattgc gttggccatg tcgccgcttg
     2761 ctgactctgc tcggtttatc cgtacggaaa tggtggaagt actgcatagt gattatgtgg
     2821 aattagcaaa agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat ggtcttcgaa
     2881 atagtttgat tccattgatg actttattag gaccgttagc tgtagcattg atgaccggat
     2941 cactggtggt agaaaatatc tttgctattc ctggtatcgg ggaacaattc gttaagtcaa
     3001 tcacaacaaa tgattatcct acgatcatgg ctgtaacgat cctttactca tttatgctga
     3061 tttttgttat tttgatcgtg gatctattgt acggtctagt tgatccaaga attcgtgttt
     3121 cagaagggag ccgaggataa atggaaatga taccaaagcg ttatgaaaca gttgcagata
     3181 tccctgcgga tgaatttctt ccattacaaa aaaataccga agaagaacgt gaacagatta
     3241 ctgcaccttc tttgaatttt atccaagatt catggcgccg tttaaagaaa aacaaagctg
     3301 ccgttatttc aatggtttta ctattggtca tcattttgat ctctgtgatc acaatctttg
     3361 tttctcctca tgatccaact gcccaaaacg tggcttatat caatttgcct cctcgtattc
     3421 cgggaatcaa tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa cttgttgata
     3481 aatatgctca agcaaacgtc ccttcaaacg taaatttctt tttaggaaca gacggtttgg
     3541 gacgtgatgt attaagccgc ttatttatgg gaatgagaat ctcgttgctg atcgcattta
     3601 ttgctgcatt actagatgta acgattggtg tagcttacgg gttggtttct ggtttgttag
     3661 gcggacgtgt cgataacgtg atgcaacgtt ttttggaaat cttatcagga attccaaacc
     3721 tcgttgtcat gattttgatg ttggtcgttt ttgaaccagg aattttttca atcgtggctg
     3781 ctatggccat cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa acaatgaaat
     3841 taaaagatca agaatatgtt ttagctggat tgacattagg tgaatcaaga atgaaaattg
     3901 cattcaaaca tattttgccg aatatctcta gtgtcattat tattcaaatg atgtttagta
     3961 ttccatctgc tattttcttc gaagcctttt taagctttat cggtttaggt cttacgcctc
     4021 cgtcagcttc attaggaacg atgttgagcg aagggtacaa gacattcctg tatctgcctt
     4081 atctattatg gattccggca gtaactttat cggttatcat gattggcttt aacttacttg
     4141 ctgatggatt gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga tgacgaaaat
     4201 tttagaagtg aaagacttag aaatctcttt tgatacgtat gctggaaagg tacgagcgat
     4261 tcgcggtgtc aattttcatt tgaataaagg agaaacattg gcgatcgttg gggaatctgg
     4321 aagtggaaaa tcagtgacga ccagaagcat catgcgcttg ctgtcaagca acgccaatat
     4381 agatgctggt caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag aaagagagat
     4441 gcaaaaaatc cgtggtaaag aaatagcgat gattttccaa gatccaatga cctctttaga
     4501 tccgacgatg cccattggga agcaagtcgc tgagtccctt agaaagcata ataaagtttc
     4561 aaaaaaagag ggacaaaaag ctgcattaga attgcttaaa ttagttggaa tacccgaagc
     4621 tgagaaacgt atcaacagtt atccgcatca gttctcagga ggtcagcgtc aacggatcgt
     4681 gatcgcgatt gcactggtat gttatccgga aatcctgatt gctgatgaac ctacaacagc
     4741 gcttgatgtg actattcaag cacagatttt ggagctgctg aaagaaattc agacaaaaat
     4801 cgatacatca attatcttta tcactcatga ccttggtgta gtagcgaatg tcgctgatcg
     4861 tgtcgctgtc atgtatggag gaagaatcgt tgaagttgga acatcggaag aaattttcta
     4921 caacccgcaa catccctata cttggggact gcttggttca atgccgacat tagatagtgc
     4981 gaacgatcga ttgtatgcta ttcctggttc tccaccggat ttattgaacc cgccaaaagg
     5041 agacgcattc tatccaagaa atgaattcgc gatgaaaatc gatgcagaac aagaaccacc
     5101 attttttgaa ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc cgcaggcacc
     5161 caaagtgaca ccgccagaag aaatccaacg tcgctgggct atctttaaag agaaacaaaa
     5221 acatacttac ggtcaaggag gaattgctga tgccaaataa actacttgaa gttaaaggat
     5281 tgaaacagta tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat gacatcagct
     5341 ttcacattta tgaaggagaa acattcggtt tagttggaga atctggaagc ggaaaatcca
     5401 ctactggccg tacgatcatt cgcttgaatg aacctactga tggtgagata ttattcgacg
     5461 gtcaagatat aacgaagatc aaagataaaa aaggtttgac gaaattccgg catgatgttc
     5521 agatgatctt tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc agagatatca
     5581 ttgccgaagg aattgatgtt aatggactag ctaaatcatc tgaagagcgt gcaaaaaaag
     5641 tcgatgatct attgagaact gttggattga atccaagcca cggtactcgt tatcctcatg
     5701 aattttcagg tgggcaacgc cagcgtattg gtattgcacg agcattggca gtaaaaccgc
     5761 gttttatcat ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa gcccaagtgg
     5821 tcaatttgtt gcaagatctc caaaaagaac atcaattgac ctatctattt attgcacatg
     5881 atttgtcgat ggttaaacat attagtgacc gaatcggtgt aatgcataac ggcttgttat
     5941 tagagatggg aacaagtgat gaaatctata atcacggtgt tcatccttat acagaaagtt
     6001 tgttatctgc tattcctcta ccagatccag accatgagag aaaacgcaga agaatcaaat
     6061 atcaacctga accagatgac ggacaagtac gtcaacttcg tgaaatcgca ccagaacatt
     6121 ttgtctatgc aactgagcag gaagtcccat attacgcaaa aaaactaaaa cgacaaaagg
     6181 aatctcttct ggttgctaac taggagggac atagatgcta aaagctttta aattccgtat
     6241 gtatccgaca gaagaacaaa aacagcaatt gattcgtact tttggatgtg ctcgatttac
     6301 ctataaccat ttgctgaaaa agcggcagaa aagttggcaa caaacaggag tagccaattt
     6361 ttcgctcaca cccgctacat taaaaaaaga atacccattc ttaaaagaag tagacagtct
     6421 tgctttagca aatgcacagc ttaatttgga tcgggctttt cgcaattatt tcaagggacg
     6481 tgccagcttc ccaaagctta aaacgaaaaa aagtatgtgg caatcttata cgaccaacaa
     6541 ccaaacccgt acgatctatc tggaaaatgg ctacttaaag ttaccaaaac aaaaagaact
     6601 aatcaagata aatcagcatc ggccagttga aggatcgatt cgctccgcta cgatttctgc
     6661 tcgatataat gaagaatttt atgtcgcact actttgtgat gtttcgccag taaaaaaaga
     6721 atctttagct aaatggatcg ggatcgccta tcatccgaag acattgatcc aaacatctcg
     6781 acctctagaa gtaactttac caaaatttca ccagacagaa gaaaagctgc aacatgcaca
     6841 gcggaaattg aatgtgaaag tccgctccgc tcaccataga aagatcaggt tagatcaggc
     6901 aagcaattat cagaaacaaa agagaaaagt aatggatctt tatctgaaac aaaagaacca
     6961 gcgagaagat tatcttgaac aattatctgg gaaattggtc aaacaatatg actacttatt
     7021 tgtagaatct tttccaaaag aagaggcaca tgctgatttt tctattcacg actggcataa
     7081 attaataaca aaattgcgtt ataagtccca atggtataat aaaaaatttc ttttgatcaa
     7141 taccgacgga gcagaagaaa gcaattccgt aagaaagagc caagtagtag aaaaaatggg
     7201 cagacattcg gttatcaagg gataaaaaat gaatgaaact gataggagcg ttagggttag
     7261 ccttgttaat aaaaagtctt aatgactttt gtttctagga atttttcttt tgaagaaaag
     7321 ctcactaatt atgacaaacc aataaaacaa tagcagaaaa agttcgtacg agctttttct
     7381 gctattgtta attttttatt cagaaagttt acgaatacat caaaattctg aaacaaaaaa
     7441 cgtaactcac aaagagttgt ttttgcgttt tttcttgaat gattaggtct gaatcttttg
     7501 ctcacaacct tattcatggt gccccggctt gagctgatag atttttgaag gacggccgat
     7561 tcctacagga cgttcaccga tctcatcgaa atattggagc atagctttct taaaattgga
     7621 atgatcgatc gttttataat cgatccctaa gaatttagca aatacttttc gagcttccgt
     7681 aatcgtgaaa tctttaccta aaacttgtag aacttgcggc tcgtgttcca ttttattgac
     7741 tacacggttg aatgctttca agatgatttg actatggtca aaagccaaag tatctttgcc
     7801 agaagactct ccagtgacta aatcaagcga gatagccact tctccgctag ataaatggat
     7861 cttgttacta tgacgttcta gtgtgaacca gcgtacttcc ttggcatcat ctccggcgat
     7921 tagcggttct tctccaataa aggctaagta gctgactgtg acgacccagc ctcgcggatc
     7981 tcgattaggc gtactgaatg tatgcagttg ctcaatattt tcttttgaaa taaccacacc
     8041 agtttcttcc tttgtttctc ttaagacgct ttctccagtt gactcatcct tttggacaaa
     8101 gccaccaggc aatgcccatg aatttcgata aggatgccct tttctttgga tcaacaaaac
     8161 tttcagctga tcttcttctt tgttatagca catcagtacg atatcgacag tcaaagaggg
     8221 cttgtcatat tctggacgtt cctgtttttg ataccagtta agaaattctt cttcacttgc
     8281 tttatgttca taatataatt tttcttcttc tttagaagca aaacggttca tctgaccacc
     8341 tccattgaca gtaaaagtac tttttacctt attgtagcag actctgaaaa aaaaccaagg
     8401 atttacttga aaaatctagg ttatcattta aatatgaaaa ataaatggaa gtatgagatt
     8461 gaataaaaag aaaagcagat aaaacggcaa tactttatac cgctcttcac gaatggcaaa
     8521 gaagccacaa ataacgatat atacgtcaat gataaaacgc aaaatccaag ctgctgtcat
     8581 aaaactgttc aaatgcccat ggaggttaaa ataaccatac atacgaatat aaaaatagct
     8641 gaatattaaa tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa gatgtttctt
     8701 ctgcatgatt gttccagtcc cttactaaga tagatagtct ttattatacc acgtgtgagg
     8761 ggagaaataa aaagccgttt tttagtagat atgaaacaaa acaaaaaccc gcatttattc
     8821 aacctattca tccttttaaa ggatagattg aacgtagata gaagtcgttt atccagtctt
     8881 tttcattttg aaaaaaagct aacagcatgc tttttttatg gtatactagc aaggaaacga
     8941 attcgcatca tgcgataaag gaggaagagc cccgtggcag aaaaggaaac tttttatatc
     9001 actacgccaa tctattatcc aagcggcaaa cttcatatcg gcaactcgta tactacgatt
     9061 gcatgtgacg caattgctcg ttataaacgg ttgatgggct ttgatgtgtt ttatctcact
     9121 ggagtggatg aacatggtca aaaaatcgaa acaaaagcag aagaactagg tgtaaaaccg
     9181 caagaatatg tagataaaat ggcggctgat gtcaagaaat tatggaaaac gcttgatatc
     9241 agttatgata aattcattcg cacgacagat gattaccata aagcagctgt tcaaaaaata
     9301 tttgatcgtt tattagaaca gggagatatt tacttaggtg aatatgaagg ctggtactct
     9361 gtttctgacg aagaattctt tacagaaaca caacttgcag aagtgtacaa agatgacgaa
     9421 ggtaacgtga tcggtggtaa agcgccaagt ggacacgaag tagaattggt aaaagaagaa
     9481 tcatacttct tccgcatgag caaatacgca gaccgtctat tggaatacta tgaagaacat
     9541 cccgaattca tccagccaga atctcgtaaa aacgaaatga tcaacaactt tatcaaacca
     9601 ggattagagg atcttgcagt ttcacgaaca acttttactt ggggagtacc agtcaagagt
     9661 gatccgaaac atgtggtgta tgtatggatc gatgcattat ccaattatat tactgcacta
     9721 ggatatggct ccgaagatga aagtctattc gaaaaatact ggcctgcaga tgttcagatg
     9781 gtcggtaaag aaatcgttcg ctttcatacg atctattggc caatcatgct gatggccctt
     9841 gatttgcctc tacctaaaaa agtatttggg catggctggc tattgatgaa agacggaaaa
     9901 atgtctaaat caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg ttatggttta
     9961 gatgcactcc gttattattt gcttcgtgct gttccgtttg ggtcagatgg tgtctttacg
    10021 ccagaagatt ttgtttcccg tgtcaattat gatctggcaa atgatctagg gaacttattg
    10081 aatcgaacga ttgctatgat caacaaatat tgtgatggac tcgttcctga ctatgcctca
    10141 cttgtcactc catttgatag tgaactgtct acgacggctg ctaacgttgt cggacgttac
    10201 catgatgcaa tggaaaaaat ggaattcaat acagcattgg ctgaaatctg ggtgctgatt
    10261 tctcgtgcaa acaaatacat tgatgaaaca gaaccatggg tattagcaaa agacgaagaa
    10321 aagaaagctg aattgaatag tgtcatgatc catttagctg aatctttgcg tatcgcagcg
    10381 atattacttc agccaatcat gacagaaacg ccagagaaga tcttcaatca actaggattg
    10441 gactctgaaa caatgaatct tgaaggatta cactttggcg aatttccttc aggaacaaaa
    10501 gtagttgcaa aaggtacacc aatcttccca cgtttagata tggaagaaga agttgctttt
    10561 attcaagaaa aaatgtctga aggtactcaa acaaacgaag atacagtcaa atgggatcca
    10621 gaagaaacag aacttgtttc aacgaaagaa aaacaaatca aatttgatgt atttgaaaaa
    10681 gtagaactaa aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc agataagttg
    10741 ctgcaattcc gactagatgc tggagacagc caagaccgtc aaatcttgtc aggaatcgct
    10801 gaattttatc ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt agcaaatctg
    10861 aaaccacgaa agatgcgtgg acaaatcagt caaggaatga tcctttctgc agaagcacca
    10921 gatggctctt tacaagtaat cgaagcacca aaatccatgc caaatggctc agaaatcgca
    10981 tagaaattca aagggggtat gacactagtt gtgtcatacc ccctttttcc gaaatttgag
    11041 aagcaatttt cgtaaattcc ttcttattac ttgaagccaa tcatataata cagaaacaga
    11101 gaactatcca aaataaatcc tagaaaaaga agtacgagct tcgctgattt ttgttaattt
    11161 agaataatac taataggaat cgtaaaaaca gaaaaaaagc ataatgataa agggtcgggc
    11221 agattttaat tgattcatct ggtactcttt tttattgtcc ttacattcat aaagtttgaa
    11281 tgcaatgact ttattacatc tatatttata tctaacagta gcgttgatgt gttaattata
    11341 agttctatca ctttatgttc aattagtttg tgctcttata tgtgtgattg aaaatataga
    11401 taaaaaaaca atcatcggct ttgacttcga tgattgtttt ctttattttg taaaaatcct
    11461 atcacttgat tttatggtaa ccttttattt ttgtcttcaa acggcttggt aagagagcag
    11521 taagcagtgc aaatgggatc gaaaagacat accagaaaat cgtacgtccg ataccataat
    11581 gttgatagag ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc aaggagccag
    11641 ctataaacca atgattatcc caatgcatga cttcactaag tgccatcact ccaaaaggat
    11701 agaaataaaa atagatcccg actaaggtca tccgccagtt ctttgtaaat gttccgatat
    11761 tcaataaaaa gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc ccaaccaaaa
    11821 aggaatacaa catcccccac aagatcatca caggaccttt gatcaatgca acgattgcga
    11881 tgaacaaagt cagcaatatt agtttttgtg ataacgttgc aacagtcatt aatccaaata
    11941 aaattaatgt gaaccaatgc caatgggact gctgttgttc tttacgggaa aaatcgaccc
    12001 ggttggataa ataatctgct aatttcggat aagaacgctt aaaatccata aactgttcct
    12061 ccttcgtgga aaaggtaggt ttagtatacg aaaaataaaa aagaaataca tccgtcttaa
    12121 gagggagttt ttcactgaaa gtggagaaaa aagaatggta aattaaaata ttctcagtta
    12181 tactgaaaat tataccaaat ttcaaagaag aaagaggatg ggaatgaaag gcaaactaaa
    12241 acgagaaatc aatctgtttg gcgcattagc taccgttatg ggaaccgtga tcggtgcagg
    12301 cgtcttcttc aaaactgctg ctgttactgc tagtactcag tctacaagtt tgacattgct
    12361 agcttggctt ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg ctgaattagc
    12421 tacagcaatc ccggaaactg gtggagctgt aaaatatatt gaagctgcgt atggaaaatt
    12481 gcccagcttt ttattaggat gggcgcaaag cttgatttac tttcctgcta atattgctgc
    12541 attatcgatt atctttgcga cacaattgac gaacttatta caattatcta ccgattatct
    12601 tttgttgatt gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt tagggacaaa
    12661 agtcggtgct tctgtacaat cagtgacgtt gattgttaaa ttaataccaa ttgctgtgat
    12721 tgtcatatgg ggattattga ccccaggaca aggaactgtc cagttatttc caatcgaagc
    12781 tggaaaagat gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa cgttgtttgc
    12841 ttatgacggc tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc cggaaaaaga
    12901 tttgcctaaa gcaattattc ttggtttgag ttttgtaaca gtcgtttatc tattgatcaa
    12961 ttttgttttt ttgaaaactt tgccgattga tcatcttgcc ggtaatttga atgcagcttc
    13021 tgaagcatca gacgtcattt ttggaggaat cggtggaaag ctggtcacta ttgggatctt
    13081 gatctcggta tacggtgcat tgaacggtta tacactaact gggatccgcg tgccttatgc
    13141 gatggcttta gaagacgagc tacctttcag taaacagctg acaaagcttt caaagaagtt
    13201 tacggttcct tatgttcctg cggttttcca gttagcagta gcttgtatta tgatgagcct
    13261 tggttctttc gactttttaa cagacatgtt gatttttgtg atgtggctat tcagtttact
    13321 gatttttatc ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac gtccatataa
    13381 agtaccactt tatccaatcg ttccgattat tgcgattctc ggtgctattt tcattttagg
    13441 tatgacgatg atgacgcaaa cgaaattagc attgatcggt ataggtgtta ccttgatagg
    13501 tatcccagtc tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata tttcaacaac
    13561 agacagaaaa agtacgaact aggtcctcta gatcttcgtg ctttttctat ttcaataaaa
    13621 caagttcttc tattttgatt tttgctactt ctgcaagtag tgcttatgtg ctaaagtaag
    13681 aatgaaaagg agagataaac atgatatttg attctcatac ccatttaaat gctgaacagt
    13741 tcaatgaaga tattccagaa acgattcagc gagcacaaga aatgggggta acgaaaatgg
    13801 cggtcgtggg cttcgatacg ccaacgattg aaaagtcttt gcaattaagc catgactatc
    13861 caaatatata cagtattatc ggctggcatc cgacagaggc tggcagttat acgaaagaaa
    13921 tcgaaaataa attacaagaa caattgactt taccaaaggt tgtagcatta ggggaaattg
    13981 gcttggatta tcattggatg gaagacccga aagaagtcca agataaagta tttcgcagac
    14041 agattgctat cgcaaaagaa atgaacctac caatcagtat tcatactaga gaagcacttg
    14101 aagacacgta tcaaatccta aaagaagaag atgttcgaga tatcggtgga atcatgcata
    14161 gtttcagtgg tgattatgaa tgggcaaaac gatttcttga cttagggatg catatttcat
    14221 ttagcggtgt cgtcactttc aaaaaagcat tagacgtcca agaggctgca gcaaatgtac
    14281 caatcgaacg attacttgtg gaaacagatg ccccttatct ggcaccagtt ccttttagag
    14341 gaaagcgaaa tgaaccaggc tatactcgtt atacagtaga aaagattgct gaacttcgga
    14401 atcttccgac agaagaagtg gctgcccaaa catggacaaa tgcgcaccga ttatttggtc
    14461 taagcgaaca tggataagat gaagatccaa gagattatag tcgttgaagg aaaagacgat
    14521 acaagaaggc tgcgggaagt agttgatgca gatacgatag aaacaatagg gtctgcaatc
    14581 aatgaagaga tcctgatgca aattgaacat gcccaagaga ctcgtggagt gatcgttttt
    14641 accgatcctg atttttcagg agaaaaaata agaaaaatca ttatggaagc tgttcctcag
    14701 gcaaaacatg cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg aagccttggt
    14761 gtcgagcatg caagtgatgc tgctatttta gaagcattga aaaaggtcgt gacgccagac
    14821 atttcccaaa cggctgtttc tgaaatcact agacaagacc ttttagacta tggtttaata
    14881 gctggggcac aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg catcggttac
    14941 acgaacggca aacaattgga aaaacgtttg aagatgtttc gtattacgcc gaaagaactc
    15001 gcccaagcaa tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa ttgcgacacc
    15061 ctctagaaca aaagagattt tattgaaaca tggtttttca tttaaaaaaa gtttaggaca
    15121 aaattttctg actgaaccaa atattttaag gaaaatcgta gaaactgcgc aaatagatga
    15181 acatacgaac gtgatcgaag ttggaccagg aatcggtgca ttgacggaac aattagccaa
    15241 gcatgcaaaa caagtcgtag catttgagat cgatgatcgt ttgatccctg tattggctga
    15301 tacgatgcaa ccctatgaca atgtgactat tgtacatcaa gatattttaa agaccgattt
    15361 gtcaacagct gttagagaaa catttcatga ggagcttccg ttaaaagtcg tagcgaattt
    15421 accttactat attacgacac cgatcatgat gcattttctt gaatcagatt tggttgtgga
    15481 tgaactagtc gtcatgatgc aaaaagaagt agctgatcgt atctctgctg aaccaggaac
    15541 gaaagcttat ggttcattat cgattgctgt tcaatattat atggaagcca gtttagcatt
    15601 catcgtacca aaaacagtat ttgtccctca gccaaatgta gattcagcga ttttgaaact
    15661 gacaagaaga gatataccgg cagtcgaagt gacagacgaa aaagcatttt tccgattgac
    15721 aaaagcggca tttcagcaaa gaagaaaaac gctttggaat aacttgcagc attcgtatgg
    15781 caaagatgac cagacaaaag catggttagc aaaaagcttg gaaacagctg gaatcgatcc
    15841 aaaacgtcgt ggggagactt tgtcattaca agaattcgcc gcattaagca atgcgatgtc
    15901 agaaaatatg cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct gtcctgcatc
    15961 aagtaataag aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt ttgggcttat
    16021 taccgcagat gcaagctttt gaacaccgtt taagaaagag gttgtgggcc tgccgtttaa
    16081 ctctaagtca caaggaacaa tttcaaaaaa cagcttctca tgtttttgag ttagttggtc
    16141 ttattacttg atgcaagctt ttgaccacta ttatgccgaa caaggagatg tgacacaaat
    16201 tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg atactcacac
    16261 tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta agtgagttat
    16321 actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga gaaaaaattc
    16381 agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta tcccaatggt
    16441 catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg tgccattatt
    16501 tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta cgacatttgg
    16561 tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc
    16621 gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa atggacagtt
    16681 tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga ttcattcttt
    16741 agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg aaacatatta
    16801 ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc ttatccacag
    16861 tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat attttgatat
    16921 ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa
    16981 gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt aatggataat
    17041 aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat cttctggcac
    17101 agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct ttatcaaaga
    17161 agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta tggaaatctc
    17221 ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc atcgtttcag
    17281 ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt gaagcttcaa
    17341 gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact tcttttcctt
    17401 cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc ttaagcccat
    17461 gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg gcaggagatc
    17521 caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc ggtgtaccga
    17581 tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc attgcttctg
    17641 tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt ttggcaatct
    17701 cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca tccattaaga
    17761 agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt tgtccaccag
    17821 ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct gctgcattgt
    17881 ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca aaagccatat
    17941 tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg gcaatatcac
    18001 ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct ccttcagaga
    18061 tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg gaaggaccaa
    18121 caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca gaataatttt
    18181 ctgcattgtc atatttttta tatacgtttt tcagagccat ttctaccata atacgttcca
    18241 cactttcttt tttagtttta ttaactattt aaagtataaa tgaaagcgtt aactaacttc
    18301 aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt ttaagcttta
    18361 ataaatatcc ataagaaata aaaactgata cactggagta aataagtatg ttaacaatat
    18421 caaaaggagt gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa aaacctatga
    18481 tggtgaaatt agcaacagcg tataaaacaa tattggaaaa acatgaactt tttgctaccg
    18541 ggacgactgg gcagaggatc acggaagcaa ccggcttgcc gatccatcgg tttaaatccg
    18601 gaccgttagg cggggatcag caaattggag ccatgatttc ggaggatcag ttagatttgg
    18661 tgatttttct tcgagacccg ctggctgcac agccccatga accagatgta acagcactta
    18721 tccgtttatc tgatgtctat gagattccgt tagctacgaa tatcggaaca gctgaggtgc
    18781 tactaagagg actggaagcc ggcttcctag attttcgaag aatcgtccat gaaatagaca
    18841 agcatccact ttcattttga tggataagcg tatggaaaaa taacgtttaa tgttcgccta
    18901 tatgtagcta taaaagctaa acaaactgat aatatgcgat ataatacgaa cgtattggat
    18961 aatataaaca ataatattag tttttcgtat ttgcagtaaa tgtgaaaata tgctatagtt
    19021 tctgtgggaa aagatcccaa aaataataca gagtagaaaa taaagcgtta agtgctaaga
    19081 ggatgggatg ttgcctcttg gacgaaggac gtaatgttcg cggttttatt ttcgcattcg
    19141 ctgcatgata cgatgtagca gctcttaaag gagatgctaa aaatgaagaa acaccggtta
    19201 gacgcacgta tgattgctat tatgggctta ttgattgcat tgatggtcac tttgtcccga
    19261 ctggtagcaa tcgagacccc atttattaaa atcagtgtca catttattcc acaagtgatc
    19321 atgggcattc tttttggtcc tttttggtca gggataggcg cagttttagc tgatttagta
    19381 ggaatggccc tcttctcgaa atctgcattc tttattggat ttacattgaa tgcatttatt
    19441 gaaggagcaa tctacggatt ctttttctat cgaaaagaga tcacttggaa aaatgccatt
    19501 ctggctacgc ttagtgtcac gctgatcatc aacttatttt tgacaccatt atggcttgcg
    19561 ttgatgtacc atgtgccatt gttcagctgg gtcgtttggg cgccccgcct gttaaaaaca
    19621 gtaatatggt taccgattca atcaattgcc atttattacg taggacgtag tattccttac
    19681 aaaaaaatt
//
LOCUS       DADVXO010000050        19633 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.051, whole genome shotgun sequence.
ACCESSION   DADVXO010000050 DADVXO010000000
VERSION     DADVXO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.051"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="KB950_001445"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="KB950_001445"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBB6857670.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="KB950_001446"
     CDS             complement(1172..1867)
                     /locus_tag="KB950_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBB6857671.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="KB950_001447"
     CDS             complement(1880..2596)
                     /locus_tag="KB950_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBB6857672.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="KB950_001448"
     CDS             2926..3534
                     /locus_tag="KB950_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBB6857673.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="KB950_001449"
     CDS             3859..4182
                     /locus_tag="KB950_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBB6857674.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="KB950_001450"
     CDS             4364..5734
                     /locus_tag="KB950_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBB6857675.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="KB950_001451"
     CDS             complement(5864..7165)
                     /locus_tag="KB950_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB6857676.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="KB950_001452"
     CDS             complement(7519..9477)
                     /locus_tag="KB950_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBB6857677.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="KB950_001453"
     CDS             complement(9654..9920)
                     /locus_tag="KB950_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6857678.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="KB950_001454"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="KB950_001454"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBB6857679.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="KB950_001455"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="KB950_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBB6857680.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="KB950_001456"
     CDS             13264..13827
                     /locus_tag="KB950_001456"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBB6857681.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="KB950_001457"
     CDS             complement(14080..14601)
                     /locus_tag="KB950_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBB6857682.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="KB950_001458"
     CDS             complement(14604..15446)
                     /locus_tag="KB950_001458"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBB6857683.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="KB950_001459"
     CDS             complement(15439..16164)
                     /locus_tag="KB950_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBB6857684.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="KB950_001460"
     CDS             16340..16636
                     /locus_tag="KB950_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857685.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="KB950_001461"
     CDS             17204..18796
                     /locus_tag="KB950_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6857686.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="KB950_001462"
     CDS             18809..19549
                     /locus_tag="KB950_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6857687.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5619 a         4028 c         3725 g         6261 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaa
//
LOCUS       DADVXO010000051        19505 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.052, whole genome shotgun sequence.
ACCESSION   DADVXO010000051 DADVXO010000000
VERSION     DADVXO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.052"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(541..1566)
                     /locus_tag="KB950_001463"
     CDS             complement(541..1566)
                     /locus_tag="KB950_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6857688.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="KB950_001464"
     CDS             1841..3526
                     /locus_tag="KB950_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBB6857689.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="KB950_001465"
     CDS             3540..4823
                     /locus_tag="KB950_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBB6857690.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="KB950_001466"
     CDS             4893..5762
                     /locus_tag="KB950_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6857691.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="KB950_001467"
     CDS             5759..6595
                     /locus_tag="KB950_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6857692.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="KB950_001468"
     CDS             6621..7688
                     /locus_tag="KB950_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBB6857693.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="KB950_001469"
     CDS             7685..8512
                     /locus_tag="KB950_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6857694.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="KB950_001470"
     CDS             8557..9582
                     /locus_tag="KB950_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6857695.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="KB950_001471"
     CDS             9579..11762
                     /locus_tag="KB950_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBB6857696.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="KB950_001472"
     CDS             11762..12892
                     /locus_tag="KB950_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6857697.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="KB950_001473"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="KB950_001473"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBB6857698.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="KB950_001474"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="KB950_001474"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6857699.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="KB950_001475"
     CDS             complement(14378..15064)
                     /locus_tag="KB950_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBB6857700.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="KB950_001476"
     CDS             15144..16682
                     /locus_tag="KB950_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBB6857701.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="KB950_001477"
     CDS             16802..17269
                     /locus_tag="KB950_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBB6857702.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17788..18231
                     /gene="rplM"
                     /locus_tag="KB950_001478"
     CDS             17788..18231
                     /gene="rplM"
                     /locus_tag="KB950_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBB6857703.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="KB950_001479"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="KB950_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBB6857704.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..>19505)
                     /locus_tag="KB950_001480"
     CDS             complement(18727..>19505)
                     /locus_tag="KB950_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6857705.1"
                     /translation="YVRSMFDIAEILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGK
                     QALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQ
                     TVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQY
                     LFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMN
                     QISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6323 a         3526 c         4144 g         5512 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatc
//
LOCUS       DADVXO010000052        19365 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.053, whole genome shotgun sequence.
ACCESSION   DADVXO010000052 DADVXO010000000
VERSION     DADVXO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.053"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            56..403
                     /locus_tag="KB950_001481"
     CDS             56..403
                     /locus_tag="KB950_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857706.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWMFYLVKK"
     gene            498..1382
                     /locus_tag="KB950_001482"
     CDS             498..1382
                     /locus_tag="KB950_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBB6857707.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1606..4773
                     /locus_tag="KB950_001483"
     CDS             1606..4773
                     /locus_tag="KB950_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857708.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4793..5338
                     /locus_tag="KB950_001484"
     CDS             4793..5338
                     /locus_tag="KB950_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBB6857709.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5366..5821
                     /locus_tag="KB950_001485"
     CDS             5366..5821
                     /locus_tag="KB950_001485"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBB6857710.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5818..6576
                     /locus_tag="KB950_001486"
     CDS             5818..6576
                     /locus_tag="KB950_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6857711.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6662..7402)
                     /locus_tag="KB950_001487"
     CDS             complement(6662..7402)
                     /locus_tag="KB950_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6857712.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7463..8716
                     /locus_tag="KB950_001488"
     CDS             7463..8716
                     /locus_tag="KB950_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6857713.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9038..9265
                     /locus_tag="KB950_001489"
     CDS             9038..9265
                     /locus_tag="KB950_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6857714.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9388..9714
                     /locus_tag="KB950_001490"
     CDS             9388..9714
                     /locus_tag="KB950_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6857715.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9782..10495
                     /locus_tag="KB950_001491"
     CDS             9782..10495
                     /locus_tag="KB950_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBB6857716.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10805..11655
                     /locus_tag="KB950_001492"
                     /pseudo
     CDS             10805..11655
                     /locus_tag="KB950_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            11776..13641
                     /locus_tag="KB950_001493"
     CDS             11776..13641
                     /locus_tag="KB950_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6857717.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13689..15098
                     /locus_tag="KB950_001494"
     CDS             13689..15098
                     /locus_tag="KB950_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBB6857718.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15206..15907
                     /locus_tag="KB950_001495"
     CDS             15206..15907
                     /locus_tag="KB950_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6857719.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16066..16545
                     /locus_tag="KB950_001496"
                     /pseudo
     CDS             16066..16545
                     /locus_tag="KB950_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17393..17968
                     /locus_tag="KB950_001497"
     CDS             17393..17968
                     /locus_tag="KB950_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBB6857720.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            17968..18408
                     /locus_tag="KB950_001498"
     CDS             17968..18408
                     /locus_tag="KB950_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBB6857721.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18424..19125
                     /locus_tag="KB950_001499"
     CDS             18424..19125
                     /locus_tag="KB950_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6857722.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         6482 a         3222 c         4152 g         5509 t
ORIGIN      
        1 agttattcac gtatacggat ttgtttttct cacaaataaa aaggcaggaa gtacaatgga
       61 tcatttgtat ataaaatata ttcatcaatt ttccgttttg gtgttaggca cactaattat
      121 agcggggatt gtcgcattta ttattcaagg ccgtgacata ttaacagtac atcaagagtt
      181 tttcagttat gcatttggta ttcctctgat cgctataagg tctgataaca aaaagtggaa
      241 gaaacgctat tacattctct tgctattttt gtgtttttat ctgccaccta agatttttac
      301 tttattaggt atacaatgcc tatcattttc ttttatttcc tatatcattg ttttaatttt
      361 cctcagtttc atagtatgga tgttttatct tgtcaaaaaa taaatgaatg tttcatgaac
      421 agtacttcat cacagcctaa tttttttctt tatgctatcg ttatattaaa gaaatcaatc
      481 agaagggaaa gaaagtgatg aaaaaagttt tattagtggt aaaccccagt tcaggcggtg
      541 aacaagcaaa ggaatttgag caattagcga tagcaaaatt ggaatcggtg tttgatgaag
      601 tggtcgtctt gcatacaaaa aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta
      661 cggaagggta tcatagtgtg tttgtcatgg gcggagatgg aacagtcaac gaaggaatca
      721 gtggaatcgc tgaacaagaa catcggccaa atttcggatt tttcccattg ggtacggtga
      781 atgacttggc tcgagcatta gggataccgc tagaacctga ggaagcgatc aatcattttt
      841 cgattgaatc agtcaagcca ttggatattg gaaaaataaa tgacgactat tttatgaatg
      901 tcgtcgcaat tggctctatc cctgaatcaa tcaatgatgt agatgcggag aagaagacga
      961 aatttggaaa actagcttat ttcatgtcag gaatcaaaca gttagcttcc acacaatcct
     1021 actcgtttca cgtagaagtt gatggaaaga aggaagaaat tgagagcagt accttgctga
     1081 taggactaac taactcagtc ggcggttttg aaacattact tccaaatgca aaagtggatg
     1141 atggtaagct ccatttggtt tatttaaaag attcttcttt gatcgatacg cttaaaacat
     1201 tgccagacct attaaaagga gtagatgaat ctacgaaaaa tctagcctac cttacttgtg
     1261 agcaattagt tgtgtcatta gctgatgagg aagaattgac gacgaatgtt gatggagatg
     1321 aaggggataa attacctgtg acaatcaaag tattgccctc acatttacag gtttattgtt
     1381 aaaatggagg ttgtgaccga ggagttggcg ggagaacacc gcttgttcgg gataagggac
     1441 tgtgacaaga cttttgtcac agcctttttt ttgtacaaaa aagaaattta cacctaatag
     1501 aaaaattttt ccataaaaat acatatttta aatatgaaag tacaattgga aatagattaa
     1561 aacgataaaa taaaattgtc aaaacgtcag agaggatggt ggagaatgaa tcaatgtgta
     1621 aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct tgatggtgac atccgttttt
     1681 gccgatgtag agaaaacaaa cgaatcaacg caatcagaga atcaaagttt tgacaagact
     1741 actttttcag aagaatcgat tacttctgaa actacagaat ctcaaagtga ggcttctact
     1801 aataaaaggc aagaagaaaa taagacaagt gagacctcag aagtagctga acaaacgtct
     1861 gatagcagtg aagcggcact ggcaagagct gtttcagcgg atgggtacag cgaagctgcc
     1921 acaacagaag aactggcgca gcttctcgca gacgaatcag taactaagat ccgactgatt
     1981 cagcctttga ctttagacag ggaattagag atcaaacgag atatcgttat tgattttggt
     2041 ggattcgctc acaatttcgg cacacaccat atttatatca atgagatacc tcagtcgatc
     2101 gagttccaga attttaaagg aacagctgca catccaggtg gattgacacc aaatatcgat
     2161 ggaaatgcca ttatcattgc ttatatgacc gatttttggg gaagcagata tcattttacg
     2221 ggagaaataa aatttaccgg gacacttgat ttgtatgatg ggtccaaatt aggactgatt
     2281 tacgctcctc gtgcaactgt cacattagat ggtgtttccg gtgtgttgga tgtccaacca
     2341 gtgaaggaag gactgaatgc cgctccgggt aaagcctatt tttgtagaag ctatcaattg
     2401 aatgtgatca atggtagtca actttatgga ccgtatctag ggaaattcta cggttttcta
     2461 gattcaggag atgagactgg tagtggaaaa acagcgccag gaatccatat cttaagcggt
     2521 tcaaaggtct ctcttgatta tgatcgggca gatggcgtta gcgatgaagg agaggcagtc
     2581 gatacgctac ctggtaatgt catgtttgaa gtcagtggtt cgggaagcga attttcggta
     2641 aatacaaaga tcaatattac caatgacaac aatcgcggaa tcatccaaat gagaggttca
     2701 ggatcgaaag tcactgtatc gaatggtggg aaaatcacca tcaatacagc aactacgggc
     2761 ggatttcgct tgcaaggaga ttctagtcaa atccatgtat caagtggtgg acaaatctcg
     2821 gtcaaaatgg ctggagatga cgatcaaccc gggaacaatg ggatgcgttt tgtaggaaaa
     2881 ggcctttctt tgatcgtaga aggagccgga tcagttatcg acatcgataa gcagagtggg
     2941 cgttccagcg cagttcgttt tgagaatggt acgcaaaagc tgacagtaac caatggaggc
     3001 tcattgaggg taagaaatac aggtgatggc aaatcttacg tgaacagact caatcaagga
     3061 aatcaagcga tccaatttta tgacgcttct ggttttctag aaagtccagg ttcagctgat
     3121 tttacagtca ctggagaaaa atcaaatatc gatatacgcg cagattccgg agcaactgta
     3181 gatacaactg gagatattga tttaaatttt acggcaggtg aaaaaactta tttagtcatg
     3241 gtcgggaaaa caggggaaga taagaccggc atcatcagtg gggatgtgtt gaatgtggac
     3301 ttagaatctc ctacctattt tgatttccaa aacaaacgga ttggaagcga gaccaatggc
     3361 ataggcggct gggtattcca aggaaatgga gattcttctt taaagatcgc ccattcagaa
     3421 atcgctttat ggcgaaaaac aggcgcaaca ttcgataact ttgaaaaaga cccggattat
     3481 ttttctgata tgacagactt atcgatcaat gggaccgact taggcaattt cgtatcaagc
     3541 cctagtgcag gtttaacaaa tgaatttgct cagacaggtg ccggaatgaa aaattataat
     3601 cgaatcagtg ccaataatca gctcccgatg attgaatcct taagagtgcc aacgaatgca
     3661 gataaaaaaa tctatgggca tgtcgttgtt cccgaaggtg tcgaggcaaa gccaagagat
     3721 gcttggacag gcgaagtaga agtgacttta cgtattactt acgcagatac gacgaaatca
     3781 tctgtagaaa ttactgctct cacgcaagga caaacagatg aaaatgaagg ggcaggatat
     3841 aacccatggg gagaaggcga acaaggcggc ttgttcgaag ttcaagtacc caatgatgag
     3901 agattaaaaa cggaggatca agtgcaagtt gttgctgcta aaaaagtaag aggtggccaa
     3961 gaagtgacag aactggactc tgccaaaacg acagtcgatg tgattcctcc tgaacctgct
     4021 aatttaagta ccacattgat caatgcagct tctcgcacga tcacaggtac aggtgaagca
     4081 ggagccttgg tttcattgaa aaaaaatcaa gctgttttgt cagaaacaac tattgatgat
     4141 acaggggaat ttgttctttc tatcccagaa aatcaacttt ttgcaggaga tgtattagaa
     4201 attgtattaa acgaccaagc aggagaagct actgctaagg gtgtgcagaa caagccaagt
     4261 accaacgatg aaattgggaa tcacaatcca agtgatacgc cagttgttta tcatgatgcc
     4321 ccgccttttg cacctgctac gaagatcgta gtagaaggtg gtctctcatt tcttgcccct
     4381 tccaaactcg attttggtca gatcaaagca actggtttga cacaagaagc atttggccaa
     4441 atggatatac aggaaaaatt atggatttat gatcagcgag aaacgaaaaa tccgtggatc
     4501 ttaacgataa aattatctca gcctttcaca aatgatgcag gattttctat gcaggatgtc
     4561 ctatatttca agaaaggaga ggagtatcaa ttagtgaatg aagaagaact ggaaattgtc
     4621 agaggacaaa attcaacgaa tgtgcaagca gattattcag aatatttaaa taacggtcgg
     4681 gcgtttaaac ttgtcttaac caaagaaaat cagatagctg gtaattactc agcagagata
     4741 acatggacat tagcagatgc gccgacagaa taaagatcaa ggaggatgat tgatgaatcg
     4801 aatatggaat aaaagtcatc gaaacaactg ctctttactt atgatccttc tccttggatt
     4861 catcctttgg cagacacctg tccatgcctc cgaattcaat tttgcggtag attttgaatt
     4921 accggctaat cagattgata agaacaaaac atatataaat gtaaggatgg agcctgggaa
     4981 aacacaggaa atcccctaca cggtctttaa ccgaacagat aaatcagtaa cagttgagac
     5041 attgatccac tccgctacga cgaattcaaa cggtgtgatc gaatatggag aatcaaaaag
     5101 taaatttgat gaaacattaa gttatgatat gaaaaactta gcaaaaacag tgggagaaat
     5161 aactgttcct cctcaaagtg cggtcactga gacactttca ctgaaagcgc cagaaaaaga
     5221 gctggctggt gtggtcgcag gagggatcac gtttcaagaa aaaaaagaga acgtttctaa
     5281 caaaagtaca gttggtgtcg tcaatcagtt ttctcaagtg aaagcaatct tgatttgaaa
     5341 tagcgaaaaa gagtcttccc cagatttgaa attaaaaaaa gcttatgcgg atcaaagcga
     5401 tggaagaaat gtgattaagg cgaatcttca aaatccaaat gcttcttata tgttcggctt
     5461 gactacatcg gttgaaatct acaaaaaaga aacaaaagag ccttaccttt caataaaaaa
     5521 agaaaaaata gatgtagcac ccaatacaaa ttttgattta cttgtcccat tagaaggaaa
     5581 aaaattagct ccgggacatt atcaagcggt tgtgaagggt gcttggaaag aaaaacgctg
     5641 gaatctccat accgattttg aaataactga tgatgcagcg gaccaattga atgaaaagga
     5701 tattgattta aaaaatcagc cagaaacatt tgactggcga tggctgatac ttgttggagt
     5761 cattcttatt ggcttatttg ctggattatt tattttaaaa gaaagaagga aaaagaaatg
     5821 aaaaaaacaa ttgcattaag taccgttcta gctggaagtc tattattatg tacacaaagt
     5881 gtttttgctg agaataagag ctcagccact acagaaggaa cgatttctgt gaccaaacca
     5941 gatgctgcaa atcctgaaga cgttgttcca gttcctcctg ttgatccaaa tgatcctgat
     6001 actcctttta cacctgacga accaaatcag ggaacatccg gattactgac gattgaccaa
     6061 gctccagatc ttgattttgg aacaatcaag ttaggtacag caaaaacagt atatgctgca
     6121 ttacaaagcg gaacggacag tactggagca caaacacaag tacagaattt cgtacaagta
     6181 acggataaaa gcggaaatta tgccggttgg aaattatcag tcaaacgaac agaatttatt
     6241 ggtacaacat ctgatacgaa taaactaact ggttcaaagg tgaaattcaa aaacgccgtg
     6301 attcgaaata gcgcaactaa tagcagtgga atgccatcta ccgtttctgg aactggcgct
     6361 gaaggaatcg aaatcccagc agatcaggca gtagatctag taactgcagg agcaaatgaa
     6421 gggattggca catggattta ttcattagga gagaatgtgg aacaagggag acaatcagtg
     6481 gaattatttg ttcctgtatc caattatgct gtagacaact acgtttcaac attcacatgg
     6541 tcgttaacag atgctcctac agaagaggaa gcgtaaaatc atatcaacga tttcagggag
     6601 tggagccgat tcaaggccca ctccctgaaa tctgctatgc ttacgtctca gctcttattc
     6661 atcaactcga agcagttttt tggaagtcat ggaaacgttc ctttgtcttt tcaaagatct
     6721 ttcgaccaac gagtaaggag tcaccgttga aaaattctaa cacataatga gcagaatcaa
     6781 ttttctgcaa ttgcgataag ttgatacagt aagattttaa aagtaaaaag taagcatact
     6841 tttctaactc tgtcttcaat tgttttaaag aagtattcac taatttatct tctgtagttg
     6901 ttttgaccat aacttggttt ttcaaacctt tgatactttc aatatataaa atttcccgag
     6961 gatacaggac gagacttcca cctttttgat caataaagat tttatcgaca tttacgatct
     7021 gctgttcttt ccaattgatc tgttctagca cttgacgaat ttgttcagat aaattcctgt
     7081 cttttctgat atatccaatc ggaaggattt tccgattgat gatctctact gcaaaattcg
     7141 tataagcggt gaaaaaaaca atattactat gtgcattttt tttacgtatt tgttcggcga
     7201 catcaatacc tgtgagtgat tgcttcaagt cgatatcgat aaagaaaaca tcgtgattat
     7261 caatagtgcc caactggatc aactgattga tttgagagat agaagtgagc tgcacgatat
     7321 tctcgatatg atgattgacc aattctctga tgatccattc gctgtgaaac tggttatctt
     7381 ctatgataaa atactttttc atatgcaggc tcctattaaa aaaatatgtg tacattttga
     7441 aacaaaacaa tggaggaata agatggtcgc aacagtgaca ctttatttga cctttacttt
     7501 gattttagat ttaggtatct atttattctt ttcgaaacaa ctgctagtaa aaaacagctt
     7561 tggttgtata gctttgatgg tggtgacact tttacttcct atccctaaag tgagctttcc
     7621 ttacctagtt ggcttattat tgatgtttct tttttatttt actacgaaaa acctgtttat
     7681 cagctgtttg atcagtaatc ttttttttgc tatcactgga ctcagctttt ttctggtttt
     7741 tgatctgatg gattatttag gaaagagaca tcttggaaca gcaagttttt ttgcactact
     7801 tatcgcattt attttctatc tattagtaaa aagtatagac aaacagacga gatttctcaa
     7861 cttgcttttt acttatccaa ctgaacacta ttttggaggt atttgtattt tattaagctg
     7921 gggcgtgatt tacttggtat tgggaagtgt tcacttccgc tcatctaatt atctgcttct
     7981 atctcttttg tcgatagtca tcagtttttt ttctttgttt ttttccatta tgttgatcag
     8041 tttccaagta aaagaaagac aacatcagga atcgcttcaa ctgtatcaaa taaatgaaga
     8101 atattaccat catctagagg aattcaaaca tgattataaa agtctactgt tttcattaaa
     8161 aattactctt caggaaaata aaaaagaaac gttggaattt ttagaaatat tagaagatca
     8221 ttctgcagct atttttgaaa aaccgcaaat acaacagcta acaaatattg tatccccagc
     8281 tgtcaggggt gtctttttaa aatttctaga aaaagcagaa tcggagaaaa tcccgatcca
     8341 agtagttatt cctgtcgaag tgaaaacaat cccgattgat ctggttgttt ttattcgctg
     8401 tttatctatt tttatttcta acgcattcga tcatcgtgta gccgatcaga gtccgattac
     8461 gatcaatctg ctggaagaaa aagagggatc acgttttagt atagcgaaca aggcaaatcc
     8521 aacgaatttg acgctatcgg agatggtcaa aagaggaaaa acctctaaaa aaaatggcgg
     8581 gttaggtcta tacagtgcga aaaaaatgtt ggatgtttac gaaaatgcag aactaaaaat
     8641 cgaattttca aaacagaatc actatttttt tgtagaaatg tatctggcat ctagaaagag
     8701 caaatcagcc tactgactta ctgtcaaagg ataagtttga ggaaagtcta cacggagata
     8761 gagacaacaa cacagcatat tcaaaagaaa cagaagcgtg atgaccgttg tcacccttct
     8821 gtttcttttg ctatgtagtt aaacaaagta gttgacactt ccgctactta gtattagtac
     8881 gaaagtatac ttcatgatac taagtagata agaagtgaaa agcggaaccc acgatcgtca
     8941 aaatgctaac tgctttgctt acaccagata aagggaagag caatatcgct tattttaggc
     9001 gtggagaagc aagcaaatcc ctacgatcac aaaaaaaatg ctgacccagc agttaagaga
     9061 gttagaacaa gatgaaagta tccatcgaaa agcctataac caagtcccgc ctaaagtaga
     9121 gtactcgctc accgaagaag gaaaatcatt aagagagatt ctagtagcgt tgtctgtttg
     9181 ggaagaagaa cgtatcgaaa aacaaaaagc ctcaagggaa gaagacacgc tactgcatac
     9241 acatggtggc tacctaaatt attgattttc tatgacattt attttaaata aaagaaaaca
     9301 ggagtttttt taaaagcatc atctattttt tatgtaaaaa aatggtatga ttaatcaagt
     9361 aagaagtgat tggaagaagg caagcaaatg aatagtatgg aaaaagtcaa ccagtttaga
     9421 gatgaacgta attggcgaca atttcataac gaaaaagatt tggctatctc tatttctttg
     9481 gaagcatctg aattgttaga actttttcaa tggaaaacgc cggaagaagt aaaagaaaca
     9541 caattagaac gaatcaaaga agaactggca gatgtattga tctattcata catgatggca
     9601 gacaaccttc acctggatct agatgagatc attgaagaaa aattgatcaa aaataatttg
     9661 aaatatcctg tagaaaaaag cgcaggtgta cgaaaaaaat acacagagtt gtagaaatgt
     9721 gtcattcggc tgttggaaag agcaaaagaa aaacagtaaa atgagtctgg agggagagaa
     9781 aatgaaaaaa aaaggaaaag caaaaagatg gctagtcaat atcctcttgt tcctcttgtt
     9841 attagtagga ctagctttga ttttcaatga acagatcaag gattattttg tcagagagac
     9901 aggagataaa tatgctatag cgaatgtaac aaaagaagat ctgaaaaaga ataacgataa
     9961 agatgtcagt tttgactttg atgcggtaga accaatgacg acagaagggg tcatgaggtc
    10021 gcaaatgaga ggaacagatc taccagtgat tgctagtatc gcagtccctt cagtttcaat
    10081 caatttaccg gttttcaaag gcttggataa tacgtcacta ctttacggag caggaacatt
    10141 atctccagat caagaaatgg gaaaaggaaa ttatgcctta gcaagccatc gtgcaacgaa
    10201 ccctgaattg ctgtttacac cgctagaaaa tttagagatg ggtgccaaga tctatttgac
    10261 agatttagaa aatgtgtata catacaaaac attttttaaa gaaaaagttg ctcctacaga
    10321 tacccaatta ctgaatgaag tcgagggaaa agaaattgtc acactaatta cttgtggcga
    10381 catggacgcc gtcacgcgac tagttgtaca aggagaactg gaaagtgtta catcgataaa
    10441 agatgcgaca gatgatatga gatctgcttt caatctagag acaaaaacat tttaggatac
    10501 tgggacataa gtcgttacag attaaaaaca gccaaaggat atgaatcgat aaggaaaaac
    10561 caatttgctg aaaaaaagag aaacgcaaag aggtgcatct ctgatttggt tgttttctag
    10621 atagacttgt gttccagttt ttttattttt tatatgaaaa ggcttgcata aataaaaatt
    10681 cgatggtatg atgattttga ataagaaatg gattgttact gtacggcagg caaaaccaga
    10741 attcagctaa gaaacagtag gcgaattcta ggttttttta tttttcttga gagggcgaag
    10801 caaaatgaaa attgataaaa ttttgaataa caatgtcgtc atttcgaaaa atggattcgg
    10861 ggaagaagtc gtttgtatgg gaaaaggact agcttttcaa aaaaagattg gggacgagat
    10921 ttcgccagaa gcggtccaaa aagaatttgt ttaagagatt cttttgctaa aaaccaattc
    10981 cagcagctga tggctgatat tccggcagag gagatcgaat gggtcaaaca agtagtcgaa
    11041 ctggctgaag ataaactaaa agttgaattt tcgcctaata tttatctgac attgaccgat
    11101 catcttcatt atgcgatcac acgagcaaaa gaaaatatcc aactgcctaa tccattatta
    11161 tttgaaacaa aaaaatttta tccgaaagaa tatcaagcag cgaaagaggc tcttttgttc
    11221 gtgaaacaaa agacgggtgt ggaactttct gaggatgaag ccggttttat tgcctttcat
    11281 tttgtgaata gccaacaagg aaacgaagac atgcaagtga cgatgaccgc taccacaatg
    11341 gtaagagata tactgagtat catcagtaaa ttcttcggca tcgtgttcga tgaagaatct
    11401 ttgaattatc agcgtatcat tactcatctg cagttttttg cccagcgtta tctcaaaggg
    11461 gaaggatctg acgagcaaga tgaatttctc tacgcattag ttcaaggaaa gtatccaaaa
    11521 gcgtttcatt gtgtcgaacg gatcaatgag tatcttcgac aaacgcaaaa acaggaaatg
    11581 gggatcgcag agcagattta tttaacgatt catattcaaa gagtagtaag tgagaaaaaa
    11641 agcatccaat cataaggatt gttactgtat ggcaggcaaa acctggattg aaaaaatagg
    11701 ttcacgaatg tttgtgactg tttttttcaa tctgggtttt tattttttaa ataaaaaaga
    11761 aagaaggaaa aaacaatgga taatcaagca gttggtcgtc gtgtatggga agcggtagga
    11821 ggacaaaaga atgtcaaaag tttagtacat tgtgctacgc ggttgcgatt cagattaaaa
    11881 gatgaatctt tagcagatac gcaaaagtta aaagaagacc cggacgtgat ccaagtcgta
    11941 caaagtggcg gtcagtatca agtagtgatt ggcagtaatg tagcagatgt ttatcaatcg
    12001 atcgtggatg aggaaggtct tgcagtttcg gaggatggaa aagaagagtc caaaaatatt
    12061 ttgaatcgtc tgatcgatat tatctctagt atatttacac cttttctagg ggcgatggca
    12121 gccgccgggg tattgaaagg attcttatca ttggcaacgg tgatgggctg gctgacagct
    12181 gatagcggta cttatcaaat attgtttgct gcagcagatg gtgtctttac cttcttacct
    12241 gtcatgcttg cttttacagc agctaaaaag ttcaaagcca accagttttt agcagtagca
    12301 attgctatgg cacttgtgta tccagctatc acagcagttg ctggtgcggg agaagctatc
    12361 agtttctttg ggatcccagt gattctttca ccatctggtt atacttcttc tgtcatacca
    12421 atcattctag ccgtatgggt tcaaagtaaa ttagagccat tcgtaaaaaa agtgattccg
    12481 caatttctgc aaatgatttt agttccgctt gtcgttttag tagtgatggt tccattaaca
    12541 tttcttgctt taggaccaat cgggacggtt gccggtaatg ctttaggcgg tctcttcaac
    12601 tcgatttatg gatttagtcc aatcgtagca ggtctaatca tgggaagttt atggcaagta
    12661 ttcgtgatgt tcggcatgca ttggggattc gtaccgatca tgtttttgaa tatcgaacaa
    12721 tatgggtttg atgtattgat gccaatgctt cttccagcca tcttggcaca aggtggggct
    12781 gctttagctg tagcattgcg aacaaaagat acgaaacttc gagcattagg gatttcttct
    12841 acagtgactt cgttatttgg aatcactgaa ccaactgtgt atggtgtgac attacctttg
    12901 aagaaaccat tcattgccgc ttgtatttct ggaggtattg gtggagctat tatcggattt
    12961 tccggagtaa aagctttctc cagcagtttg gtcagcttat tgacgattcc gactttcatt
    13021 aatacggtgg atggtgtaga gtcaaatgtg acagtagcag taatagctac aggaattgct
    13081 tttgttcttg cattcgtagg tacattgatt ttaggatttg acgaacaaac acaagacaat
    13141 caattagaaa acaaacatgc gaatgcagga gaaccgatta cttcagcacg gcatacctta
    13201 aaaagtcctt taacgggtaa agtactgcca ttgagtgaag taccagacca agttttttct
    13261 tctggcgtga tgggaaaagg tatcgctatt gatccagaag taggagaact agttgcacct
    13321 gcagacggag agatcacgac tatttttcca actggacatg ctgtagggat cacaacgact
    13381 gacggagcgg agattttgat tcatatcggg atggatacag tagaattaaa tgggaatggc
    13441 tttgagatct tagtcaaaca aggggatctc gtcaaagcag gagatttatt gattcgtttt
    13501 gacatcgaag caattagagc agctggctat agcgtaatca ctccagttgt cattacgaat
    13561 actgatgcat ttgccgatat acttgaatta gaccaaaaag agattattgc aaacgaagat
    13621 gttctggcaa ttgtaaaata aggaggaaat caggatgcaa tcacgttttt cagaaaaatt
    13681 tttatggggt ggagcaactg ctgccaaatc aattagaagg ggcttatgat cgagacggaa
    13741 aaggactatc cgttgcagat gctatgcctg gaggtaagca gcgctttgcg attatcggca
    13801 gtgaagaatt tgactggacg attgatcaag aaaaatatat ttacccgaat catcgaggaa
    13861 tcgatcatta cgatcgtttc aaagaagatc ttgccttgtt tgcggaaatg ggattcaaat
    13921 gttatcgttt ttcgattgcc tggacacgaa tttttccaaa tggagacgaa ggtactccta
    13981 acgaagcggg attagaattt tatgatcagc tgattgatga gtgcttaaaa tatgatattg
    14041 aacctgtgat cacgatttct cattacgaaa tgcctcttca tttggcaaaa gaatacggtg
    14101 gctggaaaaa tcgaaaactg atcgacttct atgaacgatt tgctcagaca gtattagaga
    14161 gatacagcag taaagtcaaa tattggatga ctttcaatga aatcaattcg gcctttcact
    14221 ttccagcttt aagtcaagga ttagtaaaaa gcaatggagc aggagagtac caaaatattt
    14281 tccaagcatg gcataatcaa ttcgttgcaa gtagcaaagc cgtaaaaatc ggccacgaat
    14341 tgcgttcgga tatccaaatc ggctgcatga ttatctatgc aacaacttac agcatcgatg
    14401 ccaatccagt caatcaggca gcaacaatga tccaaaacca agaattcaat tttttctgca
    14461 cggatgtcca agtacgtgga gaataccctg catacacagc gcgtacccat aaaaaatatg
    14521 gagtcgatcc ggaaaaatta gagcaaacgg aaaaagactt ccagttgcta aaagaatatc
    14581 cagtggacta catcggattt agttattata tgtctacggc aatcaatgag actgatccac
    14641 aagcagcaac ttcagaaggg aacttgctgg gaggagtaaa aaatccgttt cttgaggcaa
    14701 gtgaatgggg ctggcagatc gatccagaag ggctgagaat cgcattgaat gaattataca
    14761 atcgttacca gaaaccatta ttcattgttg aaaacggtct tggagccatt gatcatatgg
    14821 aagaggatgg cacgattatt gatgattatc ggatcgatta tttacgtcgt catatcgaag
    14881 cgatggctaa tgcagtagca gacggtgtag atttgatggg ctatacgcct tggggatgta
    14941 tcgatcttgt cagtgcatca acaggagaaa tgagtaaacg ttacggcttt atctatgtgg
    15001 atctagacga tgaaggaaat gggacactga atcgatcaaa gaaaaaatct tttgattggt
    15061 ataaagaagt aattgcctca aatggcgtaa acttgtaatt aaagcagctg aatgaaaaag
    15121 aacacacttc tcacatgtga tttgtgttct ttttttattt tttatgtgct attcttttag
    15181 taatagtaaa gcaaagggtg acgttatgaa aaggacacgt gtactatact tggaagtcgc
    15241 agataaaatt aaagaagata ttttctcagg aaaatatcca gtcggttcga tgttgccgac
    15301 agaaagtgaa ttagaagaac tgtttcaagt cagcaaaatc actgtccgaa aggcaatcga
    15361 actgctggca tcagatgaat atgtcgcaaa gaaaagtggt cgtgggacga cggttctaag
    15421 caatcgtcct tacaatcgat tatccaaagc ggcaagcttt acgcaaatct tggagaactc
    15481 ccgatatgaa gtccggaaag aaacgctgga tttgcaattg gtccatataa agaaggaaga
    15541 tcctttgttt tcctttttcg gtgagcaagc tgtttgtttt aagcgcttat attatttgaa
    15601 caatcagcct tatatttatt ttgttcatta tcttccacaa gaaatgatgc aattcagcag
    15661 agaagaattt ggcaaagaat ctttgtatcg tttgctgacg aaaaaaggat atactattga
    15721 ctcattttct gatgatttct cggcagtatt tttaacagaa gaagaacaag gaattctgaa
    15781 aacagacgca cagatcggca tcaaacggat acggaaatcg tctacgcaaa ttggggaaat
    15841 gattgaatat tcggaggcgg tctaccatac ggagctgcat ccttaccata ttgattatga
    15901 aacataaata aaaaaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
    15961 aaacaaaaat agcttctcat atttttcgtt tggttaggct tattaccgca gatacacggc
    16021 tgtttctgga ttaccattta ttctggataa gggggtgtgg cacgattttc agaaggaatc
    16081 tatgaaccaa tcactgtatt gattttagct tcgatggtaa ccccattcgg cttgacgatc
    16141 gcttatttct tagggaaaat cattcgtaaa aatatactca atcgtcaaga aatcgataca
    16201 ttgaagaccg cttttcctat ggggatctgt cagatcacag agggatgttt ccctatcgta
    16261 ttgaacgact tggcacgcaa tgtcatagca acgggagtcg gtgggacagt aggtggcgga
    16321 ttaagcatgt tttggggcgc agatagccgt attccggcat cgggaatgtt tgcagtcgct
    16381 acgatgacca gaccatgggc attcattggt gcattgttag ctggttcttt tgtcacagga
    16441 ataaccattt tattattgaa aaaaccagta gatccgaatg cagaaatcat ccaagaagaa
    16501 aaagaggaag aagacatatc ttgggatgat ttgacaatta gttaggagag gcagaatgga
    16561 ttacaagaaa aaagtacaat agatgtacct tgagacagga atggtatgaa agaatcagag
    16621 ttagaaagaa tcgaatatac agattttggt ttaggagcga tcgaaactgt ccagagcatc
    16681 ggcaccctcc ttgaaatgac gatgaaggaa aaatgagaac ctttcgttgt cgaaagggtc
    16741 tggtatacct tatgtcgaag gaaaggaaac aaaaaaactt tcctgtacta ttccaaaaag
    16801 gaaagaaagt tattatacag taaaaaaaga aattattctt catgctagag atcagtacac
    16861 gttcgagccc aatatcaaac actagttcca ggcaggcgca acaggtgcgg ttatcactga
    16921 attttcttcc agcagtgacg attcaagtga tatttttaca aatccagcta tttatcgtta
    16981 acaaaaagat ttttaggaga agctgatgaa caaaaatgat caaattttga tcgtttagtt
    17041 cgtcggcttt tttatttaga gaaacatcat ttatcagtga aacataacac gagaacaaaa
    17101 tgattttttc gttaataaaa gaaacaagta tttgttgttt aaaataatac acgttattgg
    17161 catataggac gaaatcccag tatcttctat aagtaagtca tttcatatcg atgaaaacac
    17221 gttatttatt tttgttttat ctataaaatg gctgaaaaat gatttttttc taaatactat
    17281 atatagtggc gtctatccta tataccacta aaaaactaca aaatctagtc ttttatttta
    17341 aaaaaacacg tgtagaatga agatagactt ttgagttagg gagtgttttt ttatgaaact
    17401 tactgttttt tgcggttccc gttttggtaa taaagaaagt tacaaattta ttgcgcaaac
    17461 gttaggaaaa tacatggcac aagaagaaat cgaactggtt tacggtggtt catactccgg
    17521 gattatggga gtgattagta aaacagtact ggaaaataac ggaaaagtca caggtatcta
    17581 tccgaatggg ctttttgaaa ctgaattgcc tggaaaagat gttactacat ttattcctac
    17641 agaaacgatc gatgaaagaa aggtattatt atttgaaaag ggagatgctg tcctcgtttt
    17701 ccctggaggt ctaggtacat tggaggaatt ctcacaactt ctttcttgga tagcaatcgg
    17761 tctgacgccc gacaagccaa tcggaatatt gaatattggc ggttattaca gcggcttgca
    17821 acaattatta gaaacttttg caaaagagga ttttatggat aaaaaatggt tggatcaagt
    17881 aatcttttcc aacaatccat tggaactggt caatgaatta cgagcagtat ctattaaaaa
    17941 tcagttatta ttgaaggagg caaaataatg agtcaaatat atttagcagg accatttttt
    18001 tcagaggagc aaatcgatcg agtaagtcgt atcgaaaaag cgttggagga aaacaaaaca
    18061 gtcacaagtt tctactctcc acgtcatcac caagaaagca attatgaact attcagcgct
    18121 ggatgggcgc aggaagtata cgaaaaagat atggaagaac tgacaaatgc agaatttgtt
    18181 gttgctatcc ttgactttga gcatcaaaca atcgatccag gaacggctta cgaattaggt
    18241 gtggcaacta tgttgaaaaa accgatgatc attgtacaag aagagacggt acctacgaac
    18301 ttgatgatta cacaaagtct ccatacgtat ttaaaatcag accaagctgt acgtgaatat
    18361 gattttgaaa cattgcctgt tgaaacttac gtaggcgagt acttatagaa gaggaagggt
    18421 tgtatgtcag tgattttgat ttcaggaacg attggtgcag gtaagagcag tttgacggat
    18481 atgcttgcaa aagagattga ttctaaacca ttttatgaaa atgtcgaaga taacgaagta
    18541 ttgccgctct tttattcaaa tcctgagcag tacgcttttt tattgcaaat cttctttttg
    18601 aacaagcgat ttttagcaat gaaaaatgct ttagtgaacg atgacaatgt gctagaccga
    18661 tccatctatg aagatagcct gttgttccat ctaaacgctg atttaggtcg ggtaacggat
    18721 atcgaagtac agcagtacga tagcttatta gatacgatgc taaacgagtt agatgatgtt
    18781 gctccaaaaa aacggcctga tttgatggtc catatcaaag tttctttgga cacgatgcta
    18841 gaacgtatca aaaaacgtgg gcgtgactat gaacagctag aaagcgatga gacgttatat
    18901 acctattatg aaacgttgaa cacccgctat aatcaatggt atgaagattt tgatgtatgt
    18961 cctaaaatac aagtggatgg ggataaattc gattttgtag aaaatccaga agatgcaaaa
    19021 aaggtgatcc gtcagatcca agaaaaatta gctgaattgg aaggaaagcc tgctggaaaa
    19081 tattttacgg ataaccagca tgcaaaaggt caagaattag gctaaaaata aaaaaccaaa
    19141 cgacgggaaa gaaaaattac tacgtgatgt agaataagcc tttctatcgt ttggttattt
    19201 tatattcaaa tatttgaaat aaataaatct ttactctttg atttgtaaac tgaaatgatc
    19261 cactgtttgt tttactgatg aagcttgatc aaaggaagaa gctgagtttg aagttcataa
    19321 aaatcagcca tagtgagttt tgcttggatt tgtttatctt cttct
//
LOCUS       DADVXO010000053        18931 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.054, whole genome shotgun sequence.
ACCESSION   DADVXO010000053 DADVXO010000000
VERSION     DADVXO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.054"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            172..1047
                     /locus_tag="KB950_001500"
     CDS             172..1047
                     /locus_tag="KB950_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857723.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1192..1344)
                     /locus_tag="KB950_001501"
                     /pseudo
     CDS             complement(1192..1344)
                     /locus_tag="KB950_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1612..2943)
                     /locus_tag="KB950_001502"
     CDS             complement(1612..2943)
                     /locus_tag="KB950_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6857724.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3092..3805)
                     /locus_tag="KB950_001503"
     CDS             complement(3092..3805)
                     /locus_tag="KB950_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6857725.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3980..5404
                     /locus_tag="KB950_001504"
     CDS             3980..5404
                     /locus_tag="KB950_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6857726.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5703..6410
                     /locus_tag="KB950_001505"
     CDS             5703..6410
                     /locus_tag="KB950_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBB6857727.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6397..6720
                     /locus_tag="KB950_001506"
     CDS             6397..6720
                     /locus_tag="KB950_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBB6857728.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6809..7846)
                     /locus_tag="KB950_001507"
     CDS             complement(6809..7846)
                     /locus_tag="KB950_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6857729.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8167..9150)
                     /locus_tag="KB950_001508"
     CDS             complement(8167..9150)
                     /locus_tag="KB950_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBB6857730.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9565..10335)
                     /locus_tag="KB950_001509"
     CDS             complement(9565..10335)
                     /locus_tag="KB950_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857731.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10328..11263)
                     /locus_tag="KB950_001510"
     CDS             complement(10328..11263)
                     /locus_tag="KB950_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857732.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11579..13228)
                     /locus_tag="KB950_001511"
     CDS             complement(11579..13228)
                     /locus_tag="KB950_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBB6857733.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13345..15948)
                     /locus_tag="KB950_001512"
     CDS             complement(13345..15948)
                     /locus_tag="KB950_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBB6857734.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15960..17009)
                     /locus_tag="KB950_001513"
     CDS             complement(15960..17009)
                     /locus_tag="KB950_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBB6857735.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17035..17349)
                     /locus_tag="KB950_001514"
     CDS             complement(17035..17349)
                     /locus_tag="KB950_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBB6857736.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17365..17811)
                     /locus_tag="KB950_001515"
     CDS             complement(17365..17811)
                     /locus_tag="KB950_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6857737.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17812..>18931)
                     /locus_tag="KB950_001516"
     CDS             complement(17812..>18931)
                     /locus_tag="KB950_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6857738.1"
                     /translation="FSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQ
                     ELVRIKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVD
                     LLEEIEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRIN
                     NGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQ
                     YLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIK
                     REKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPE
                     LNKIYETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         5795 a         3805 c         3100 g         6231 t
ORIGIN      
        1 taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa
       61 tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta
      121 aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat
      181 tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt
      241 tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa
      301 gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat
      361 gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt
      421 ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt
      481 tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa
      541 cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc
      601 atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc
      661 atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat
      721 cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag
      781 cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta
      841 ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc
      901 gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt
      961 cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta
     1021 aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca
     1081 agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga
     1141 cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc
     1201 atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata
     1261 cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca
     1321 acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa aataacgatt
     1381 aataatatta acagtcttta tttttcacca ccaaaaacct atctttgcat atactaaata
     1441 gacgtatata cacagatagg tttgtcttgt gaaaaataat gattatcctt accaaaaaat
     1501 gtttgttttt ctacatgttc gtacttatca aaaaatatga tccttattga aattactggc
     1561 tatttatatt ccaacgactt cttgtgttaa tttttgttat ctcatagtaa attatgtttc
     1621 attttcactt aatttagttg tcacgtgttc gtcaattcgt aaaaacggta aatatataaa
     1681 cacacttgcg gctataatta atatttgtaa aacagctgct cgccagtctc cggctgttgc
     1741 taaaaatccc gatataactg gtggcgtagt ccaaggaatt tgcactacta catgattaat
     1801 taatcccaca gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg caagcacaaa
     1861 gggaataatc agactgatat taaatacaat aggcaatcca aaaataatag gttcgcttat
     1921 attgaatata gcgggagtta ccgataattt agcaaagtcc ttataatctt ttctacgact
     1981 gaagataaat attgcaatca ataaagcaat cgtatttccc gatccaccca ttaccgcaaa
     2041 ggtatcttta aaggccatat tgataatatg tggaatttgc tctttgtttg cataagctgc
     2101 catattttct tgcatattct gaatcaaaaa cggatctaac aatgaacctg atattactgc
     2161 ttgatggata ccaaatccaa aaaatagatt agcaatagat gtaattaaca aaaatcctgg
     2221 taaaccagtt gtaattttac ttaatggctc tgtaactatc gtggtaatta gcttatttat
     2281 atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg taatcgtaat
     2341 aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat ttccggatag
     2401 ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag ccgctaatcc
     2461 aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta tcacacccgt
     2521 cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag gtgttaatac
     2581 aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat ttctgcttgc
     2641 acacatatga taagatactg ctgctacaac taataatgta gttatgctta atgtaccatt
     2701 ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga caatttcccc
     2761 catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc ctgctaacat
     2821 cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga tctgattccc
     2881 caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc tacttttgcc
     2941 catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca aataatacat
     3001 cacttatcaa aaaaagtaaa tacttttaat taaatatata tatctttttt agtataaaaa
     3061 aataagctct gaaaaaataa ttttcccgag cttatttttc aacaaaaaca gcttcaaaaa
     3121 caaagtcttg atataaatgt ctcgataccg aatactcgaa aggtttacca gacgatagag
     3181 caacaacttt gtctatctca atcataaatt cacctggatc tatttttaat atttttgcgt
     3241 caaattcatt cgcagtatct cctttgatac gtatgtgcga agtctttatt tccagaccca
     3301 gttcatttgt gatataagca tatacagatt tctttaaatg ttttggttcc aagcctggta
     3361 tgactgctaa tggcatgaat gtttgctcaa tcgagtatgg ttgacaatca ataaaacggc
     3421 ttcgtacaat attatataca tagtctttta cactgatact taatttatca gctacttttt
     3481 tcgatggaag ttcaattgag aaagtatgga tttctgaatc tacgtgttct tcaccaacca
     3541 cgctaaacaa accttcgttt ggaccaagaa ctcgtgagtt atcaattggt gttctgacga
     3601 cttcagtccc atacccactt ctacttcgca aaactccttc ggaaacaagt aagtctacgc
     3661 ctttctttac tgttaacttg cttgtatgat aaataactgc caattcatct cctgtaggaa
     3721 gtttctcacc aattttatac tctccattta caattttttt ctttatatct aagtaaattt
     3781 ccatatactt cgcaattttt ttcaatggtg accctccaat caaaaacata tcttcatcta
     3841 tactttaaca taaataaacc gataaaatcc acatcaaaat tatacacaca ataaaaaaga
     3901 tatatatatt taaacaaaac tatttacttt aagttgtaag cggtgttatc attaagtgga
     3961 aacaggaggt aaaaaaatta tgacaactat gaaaaagcta acttttccag atgatttttg
     4021 gtggggttcc gcttggtcag ctgagcaagc agaaggaaaa ggagaaacag gaaaagcaga
     4081 gactatctgg gatcgctggt ttaaagaaca accttaccgg ttttatcaac gaatcgattc
     4141 ctctattaca accgatcaca tccatcaata taaagaagat atagaactaa tgaaaaaaac
     4201 tgggcataac tcctttcgcg tttctatttc ttgggcacga atgtttccaa atggcggtac
     4261 cggaaaagta aatcccaaag ccatttcttt ctatcgaaaa ttattttccg aaatgaataa
     4321 taacggaatc aaagtatttg ccaacttata tcattttgac atgcctgcta ctcttcaaga
     4381 taaaggagga tgggaatcac gtgaagtagt tgatgcttat gttcattacg ctgaaacttg
     4441 ttttaaagaa tttggtgact tagtttgtca ttggtttacc ttcaatgaac cacttgggcc
     4501 tattttaggt acttatcttg aagattttca ttatccaaac ctcattgact ttaaacgcgg
     4561 agcgcaagca gcattcttca ctatacttgc acacgcaaaa gcaatacaag tatttaaaga
     4621 actagctctt cctagtaaaa taggcgttat tctaaatttg agtcctactt ttcctcgtag
     4681 tcaaaatcct gctgatgtga aagcagcaga aatcgctgat ttattttata cgcggagctt
     4741 tttagatcct atggttaaag ggaatttttc taaaaagctt atcctgttat tacaagaata
     4801 taatcagctt cctgaagatt atacgaaaga agatcttgta tgtatctacg aaaataccgc
     4861 tcaaattctt ggactaaact attacgagcc acgtcgcgtc aaatctcgtt cttctatggt
     4921 ggatacatct agtccatttt taccagagtg gtttttcgaa ccacatatca tgcctggacg
     4981 acgaatgaac gaataccggg gatgggaaat ttacgaaaaa ggaatttatg atttgtgcat
     5041 ggatatcaag aaccattatg gaaacatcga agcatttatc tcggagaacg gaatgggcgt
     5101 tgcggatgaa gaacgtttca tggatgatac agggcaaata attgatgatt atcggattga
     5161 gtatatcaaa gatcatcttg cttacttatg gaaggcaata aatgaaggat gtaacattaa
     5221 ggggtatcat ctttggacat ttattgattg ctggtcatgg atcaacgcct ataaaaaccg
     5281 atatgggctg gtttcactcg accttactac tcaaaaaaga acaattaaaa aaagtggcga
     5341 attttacaag caattaagtg atacaaatgg atttagatac gatgtagact tgttagttga
     5401 ataatattct aaaattccac ttgaagtgaa cacattagaa aacaagaata atgagaaata
     5461 ccaataacaa atactttttc ttcatacttc atttatactt gtcagtataa gagactgtga
     5521 caaatgtagt gatcctcaga agttaggtct ggaaatctaa cttgagaaga tttttttaaa
     5581 ctacgtccag tcttttttat ttgtttaaaa ttgaagttga caagtcttca ttaaaaattt
     5641 agaatattca caagtttgac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt
     5701 tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct accgtttttg
     5761 gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccggg tttcatccgc
     5821 tggtcgtcac gttgatgtcc ctgctgatct atgctggttc tgcccaattt atcacggtca
     5881 gcatgcttgc tagccatagt ccactgctct ccatcgttct ctcgaccttt ctagtcaatt
     5941 cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc
     6001 aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat
     6061 tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt
     6121 atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc
     6181 cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc
     6241 aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt
     6301 tgattctggt atatgtcgga ctgattttta tcccaagtaa cttagtgatt ttagttgtaa
     6361 cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg
     6421 ctgactattt taggttgtac tttagcaact tggctttcta ggatcctacc atttgtctta
     6481 ctgaaaaaat tcgatttgcc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc
     6541 attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag
     6601 atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa
     6661 agcttattgg tgatcgtctt ggtaggaatt ctttcccttt cgttgattcg cttattataa
     6721 ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca
     6781 aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt
     6841 ttcctaatat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca
     6901 tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag
     6961 caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga
     7021 acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct
     7081 ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgt ttgatttgat
     7141 caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt
     7201 cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt
     7261 tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg
     7321 ctagttgatt caaaatcgaa ccgacgcccc cagctccgtt gatcactaag atttttttcc
     7381 cgcggttggc attttcttct ggagtcagac caaatttttc aaataacaat tcataagcgg
     7441 tcaatgaggt caacggcaag gctgcggctt cttcatctga aagatttttt ggtgcaagag
     7501 cgacaatacg ttcgtcaacc agttggtatt cttgattgct tcctgctctt gtcgttgtac
     7561 ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcatcacct acagcaacaa
     7621 ctttcccgac tccatcaaac cctaagatac gtaaagcatt tcttatcccg ttatcttggc
     7681 gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg
     7741 aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca tcgatcgaca
     7801 acccttcata aaaaccgact accttcatta attccttttc tgacatacga attcctcctt
     7861 agcttcatca agttggagca tccatacagt tttcatttca atatatatag tatgctattt
     7921 gttccacttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
     7981 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttttaaacg gtgttcaatc
     8041 aactgaccct cggtattcgc tcaaaaaacg acaaaatatg aggagctatt tttgtttggc
     8101 tgttcttatt acttgatgca ggacagcttt ttcacgacct ctaattcacg gtgttcaagc
     8161 aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtgt ctaagatctc
     8221 tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat ttgctcctgg
     8281 aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac gcgggatgat
     8341 ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca gttctttttc
     8401 tgtatgttcc ctcggttctt tttcaaatgc ataaggattc ttgaaaatcc cccggccaat
     8461 catgatgcca tctacgccat atttttcggc caattcttct cctgtctggc gatctgggat
     8521 atctccgttg atcgtgatca gtgtttgcgg agcgatttgg tcacgtaaag caatgatttt
     8581 agggatgact tcccaatgtg catctacttt actcatctct ttacgcgtac gcaaatggac
     8641 agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca tctcagacat
     8701 ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt tagccgcttc
     8761 aatcaattgt gccgctactt ctgggcgaag gatcaagcca ctgccttttc cacgttctgc
     8821 tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca tttctgccat
     8881 agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg caaccatcgg
     8941 ctgttcatct tctgtaaagg tcaatcttcc tcgtacactg tcttttccat ctggatgaca
     9001 gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg catgctttac
     9061 gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa aaaaaggctt
     9121 tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc tatcaagcag
     9181 tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa aaagaagcaa
     9241 agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa gactgtgaca aaaatcttat
     9301 cacaattcct tttccggaat aaatggtgtt caaaagcttg catctgcggt aataagtcca
     9361 accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa
     9421 cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
     9481 caacaaacga caaaatatga ggaactattt tcccgtttgt ttctctaata ctcaggtcaa
     9541 aacgctgatt tcacaacctc ttttctaact tctcaatcct cttcctttat tgatcaaata
     9601 ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg atgttgctac
     9661 tggcacatcg attgttccaa ggaatccgta acggaaaccg gagatcatat aaacgatcgg
     9721 attgactttc gaaacagctt gccaaaatgg cggcaacata gagatggcat agaaaacgcc
     9781 acctagataa gttaacggct gcaagacaaa tgtaggcacg atcgatacat cgtcataaga
     9841 ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac aagattgccg tcatcaataa
     9901 tgtaatgatg accatgaacc atgaatacac atgaagtggt acaaagaaca aggaaatgat
     9961 cgttacgaga gagccgacga gaatactgcg acccactccg ccaatcacaa agccccaaat
    10021 gatcacatgc gtaggaacag gcgctaccaa gatttcttcg atgtttttct ggaatttttg
    10081 agagaagaag gacgaagaaa cgttggcata cgaactagtg atagcagaca tcataatcaa
    10141 accaggcacg atgaattcca tatatgaaaa accacccatg tcaccgatcc gaccaccgat
    10201 catttttccg aaaataacaa agtacaagga tgtcgtgatc acaggcggaa ccagtgtttg
    10261 gacccagata cgcaaatagc ggttcgtttc cttgatagct aaacttttta atgcagtaaa
    10321 ataaagatta aacatttttc tcctccgtat gattattttc ttctgtgatt ttcaaaaaca
    10381 gttcttctaa acgattcgac ttattcctca tagaaagaac tttgattcct tgtcttgtca
    10441 gctgatcgaa gattccgttc accccttgat ttcgttcaac ttcgacagcg attgtctgtt
    10501 catcttcaaa tgtacaagcg tatccttcaa tcacaggttt ggtctcatat ggcgctaaat
    10561 caaagataaa tgtttcaaat tgcagtttgg atagaagctc tttcatacta gtattctcga
    10621 tcagttcacc tgattggatg atcccgatgt tgcgacaaag catttcagct tcttctaaat
    10681 aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa ttctcggaga aactcccaca
    10741 tctcgcgtct caattcaata tcgactcctg ctgttggttc atccaagatc agcaaacgtg
    10801 gttcatgcat caatgcccta gcaatcatca gtcttcgctt cattcccccg gaaagcatac
    10861 gtgcacggac atttctcttt tcccaaagat tggactgctt caaatatttt tcgctgcgtt
    10921 tcatcgcttc tttgcgagag acaccgtagt agcctgcttg attcacgacg atttgctgga
    10981 ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa tccaatctgt tgctttgctc
    11041 ggactagatc cgtatccaga tcataaccga agacattcac ttttccagat gttttattga
    11101 ctaaagaagt aatgattccg atcgttgtag acttcccagc tccatttggt cctaatagcg
    11161 catagaaatc tccttctttc acggaaagat cgattcctcg caatgcttcg accccacttc
    11221 cataaacctt tttcaaatta tttatttcaa gcgcatttgc catttatttc ctatctcctt
    11281 atagacatga ttctttattg tatcatggaa tacagcagat gaaaaaacaa cttggttcca
    11341 gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat gaaagacatc aatcggacga
    11401 agtcagacaa ccttttcaga aaaaacaaat cgaagataaa ggattactat tcatatgtgc
    11461 aacaaaagga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    11521 aaacggcgga agcaaaagcc aagatttttg atccaataaa tactttcgat cacttcgcct
    11581 atcccaatac atgccactgc cgcttttcat caaataacgc ggcttccgtt tttgcggttt
    11641 ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt cgggtccttg
    11701 aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt acttgttctt
    11761 caaagtggac attctcacaa taattcttta aaatctctgt caattcgata tcatgtgtgg
    11821 cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg gaagaagcaa
    11881 tccgttcgat cgtatttgtc ccttttaaaa tctcatctac gaagcagtag cagcgttctt
    11941 tggaagccac ttggtccaac aaacgtttga tcgatttgat ttctgaaaca aaataactgt
    12001 cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg
    12061 taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg atggctatac
    12121 ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta ttcttctgcc
    12181 agttgaccgg attcactacc gcttctttta acaatggatg atagatatct ttcgctgtca
    12241 caccgccctc agtaaactct ggtcggcaag tgatgggcat ataagtacgg taattcaata
    12301 ttgcagcggc tacttctaat tctcccagca attcccacag cttgatggct tcagttgaat
    12361 gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg aacatgatgt
    12421 tgacatattc aaaaatcaac tctgcttcac tcccactctt catacgaaat gaaaaaccta
    12481 atttagggat gtctcttaat ggtttagttt gctgtttcag ctcttcttga agtggtgtcg
    12541 gcagttttgc tagctgattg gcggctgaaa tcgttgaaac caaataactc atgctattca
    12601 gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat actaacccac
    12661 cgattgccaa atagattcct agtacttgtc ctaaaagaag caaacccaaa cccaccagcg
    12721 gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt tttccatccg
    12781 ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat tgatattgga
    12841 ttttttctct cagttgagga ttttcctgat aaaaagcaat cagtttttct aactgctgat
    12901 cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct acactagaat
    12961 aggtactgtt gattccttcg aatacttcga acatatccag gtcataccaa gtaagatcat
    13021 cgatttcgct atcccatgaa cggaacttct tctccgtgag ccatgcttcc ttcaagcttt
    13081 cttctttatc aaatctcggt tgataaggaa ttttttccca tttgctacga actgtttcct
    13141 ttaatttcaa acgtgaaaaa atgtctaaag caactaataa aataacagct gcaataataa
    13201 ttaaaacgat aaattctctt tgacccatat ccctctccgt tcctttattt gttagcttta
    13261 gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa cacctttcaa aataaaaggt
    13321 gtttttttct tgtatattac atatttatct ctcatttatc aaaattgaca aataatcata
    13381 gggaggaatt gttgttaatt cttgtttatt ctccagtgca ttgacaactg tcccaaggga
    13441 aaggatagcc tcaggtacta ttttttcttg ttctgttgga ttagctaagc gaagaacaaa
    13501 tccttgttca tgaaaataag aaggataaat cgatgataca attaagtctt caggtaattc
    13561 aatcaatgaa aatttacgag gaacatcaag ttcattttgt aatggccaaa tcttgttatc
    13621 taacctgtga ataaatacat tcagtttctg cttttgataa gaaatagatt gttctaaccg
    13681 tttttctgcc tgttttgcca ctttgaactc attaaatctt ccttcttcta cattaaatcc
    13741 aaaagtgact ttatactcac ctatctcttg agctagcggt gtttccatca tgacatgccc
    13801 ttcatttgtc gtatctcctg aagctcgccc aggtcgccaa gcaagattag gttttccaag
    13861 ttcccctgtt gttgaaaata aggttatgat taattgattt tctatacgtt catactcttt
    13921 taaaccatct gcataaaacg taaaataatg atcattttct gccacagaga cactcttatc
    13981 gaatatttct aaattaacag gtttctctac catagtttct gaccaattcg gaggaatgac
    14041 atcaggggta ttttcaataa atccgttttg aatttgtgca attgattttt cagatttatc
    14101 taaaacatta attgctaagc gcatacgatg cgagtaaaca gtattatgaa ttttgagtgt
    14161 gccttcaacg atttgacttt ctcccagact gaacgatatt tcataagata tttcttccat
    14221 ttcgggtttt tcagataaac gatcttttaa atctttagga agttgagctt ttccgtaaac
    14281 aactaatgtt tcttgtaaag aatctttata tactttagct gtttctaatt ttagagatag
    14341 ttctgtatct ccttctaatg gtgaataatc atacgtatcg ccatcatttg ctgaatcgat
    14401 caaatgaacg aaatcaagat attgtctatc tccaactatc aaattcaatt gcccatcttt
    14461 aaaagaaagt ttatatttat tgttacttat ctcagttttg tctatctcga ttgctgtttc
    14521 taatttttca ttatttttct caaattcaac aacagtgtat cctagtgctg gcacatttac
    14581 atttattttt atgtcaagtt cataataagc tggttcatca aaatattcga aacctgtagc
    14641 aatcatccga taagcatttt tccgttctgg aaaataaact tctttttcaa tgatattata
    14701 gggactacct ttgaagtgta ttttctttga atcagctact acatgcacga ttttcgatcc
    14761 actataattc tcagtcgttg tgttgaaaac aaggacttca ttcgatttta gctttaatgc
    14821 atcagccatc cgttttacaa taaggttttc gattccgtca gctatctcat tagcttcttt
    14881 gattcgatgg aaaatatctt ccgcaacatt atctgaaaca cagccaccaa tactatcatg
    14941 agcctggttt tgcaaaatct ttttccataa ccgacgaagt aggcctttac tgatatggat
    15001 accatttctt tctgcaatca gcatgagcgg ctcaactcgt tttagaattt tttgttccaa
    15061 tttaaaattt tctattttca tttttgttct tactgagcca atagaacgat gaacacgaga
    15121 gtaaaccggc tgacgaagtt cacctttata ttcaggtaat tcttttttgt ttctgataac
    15181 ttctacataa gaaggataat ctgaaataat attgtggaat ttactgtatt tattcaactt
    15241 ctctacgact tgatcaaaat ttaaaatcat tgacttttga tctacacccg aaggtactaa
    15301 aatatctttg ttttttcctt gcatatgaag catttcaact gcttcatcca acttttcttt
    15361 tgcaaattca ggaatatctt taattgcgtc taaggtcatc attccagtca tataaccaaa
    15421 tggaaaattc atggcataaa caaatttatc tcccaatcct ttccagataa aataaggaga
    15481 tggaaccatc gtttcaaagt ttaatcctcg ccaacacatg aaattatcga tttctacttg
    15541 atgcaaaagt gtaggcaaat tcgcattgaa cccaaacgtg tcaggcaaat accctaccat
    15601 cattggatct ccatatttaa ggatcgtatc actgattcca atcattaagt ttcttaaaat
    15661 tgattcagca tccactaata aggcatctgt ttgtgcatac cacggtccca cgaatagttg
    15721 tcctgttttc atcagtcgtt ggataacctt aagcttttgc ggattgatct ccaaatactc
    15781 atcgacgatt gaagtttgtc catctaaaca aaagtttact ttagggttcc tttccaattc
    15841 atctagaact tcagtaaaga cctgatcaga caatacgatg gcatcttcat ttgtaaaata
    15901 ccattctctg tcccagtgcg tgtgttgaat tatatgtgca tttaccatat cagattctcc
    15961 tactttcctt cttctactga atttacttgt tgttcttttt tatacttgat ggattttagt
    16021 gcattgacca ttaacgctgt aaccactccc ccaataacaa ttgatactaa atataaaggt
    16081 atattcgtaa caaaaggaat tgccataaag gttgaaagca ttgtttcttg agtaacacca
    16141 aaaaatccat ttagaccaca tgctactgca gtgcccacca cgatgcttgg tattactcgt
    16201 aaaggatctt tcacggcaaa cgggatcgtc ccttcagtaa tccccacaag gcccataatc
    16261 atagctgtct ttgcattatc tcgttccgat tcatcataaa gttttttgtt taaaattgtt
    16321 gctaaaaaca ttccaaatgg cggaatagca attcctaata acatggctgt actaggtcca
    16381 aagcttccgc ttgaaaaaga agcaacccca aaagcatatg cgattttatt gataggtcct
    16441 cccatatcaa acgccatcat tcctcctaaa atagccgaca tcaaagcagc atttgctcct
    16501 gacatgcctt ctagccaatt agtcagtcct gttgtcattg cttgaatagg cgtagctagt
    16561 acataccata atatgattcc aacaactaat gtagtaacaa aaggaatcag cataattgat
    16621 tttagactaa taacttgcat cggtactttt acttttttca atgcattagc taaatagcca
    16681 caagctaatc cagcaaccat tcctccaata aatcccatac caagattttg acacatcatc
    16741 ccagtgataa aagctggtgc tagaccggga cggtcagcaa ttgaatatgc gatataggct
    16801 gaaatcatcg gtacgataaa ctgtaatccc gtctgaccaa tcattcgcag gactccccag
    16861 aatgtatccg tctggtccca aacacctgtg ccaccaaaaa tgactgcaat agaagttagt
    16921 aaccctgctg ctacgacaag cgggatcata taggaaaccc cactttggaa atgatttaga
    16981 atttctcttc cttgtttctt gaattccatt agtaactccc ccacgatata ttttttattt
    17041 gttaattaat gcaattgctt catcaatcgt ctcttcacca tcttttacta cctctgaggt
    17101 ccctactgtt aaaatcggtt taccgtcaaa gcgacctcgt tctgaaacat ttgtatctac
    17161 tgcaaatatg acaacatctg cctcggaaac atccgaaatt gaaagtttgt tttctatccc
    17221 catagccccc tgtgtttcta ctttaatatc taaccctttc ttcttcgcaa acttttctaa
    17281 attagctttt gccatataag tatgggcaat ccctgcgata catgctgtta ctgctactat
    17341 ttttgtcata tgaaattcct ccttttaaaa tgatagagct tttaccaatt cttttttatt
    17401 tctcgaatct tttattcttt taacaaactc gtcatccatc aatcttccag ataattctgc
    17461 taaaatacgt aaatgattag tacctttatc agtgtctttt attgctaaca aaaagacgag
    17521 atcaactggt tgatcatcta atgcgcccca ttcaacagac cgctttaact tggctacagc
    17581 cactgttgat tcaattactg ctaggctttt gccatgagga atggcgatat ttttcccaat
    17641 tcctgtggtc atatggtttt ctctgtcaaa tacgtctttt tgaaattgtt tgtggtcaga
    17701 cacaaaccca ttagtctcta acaatttcga taatttttcg actacacttt ttttatcaga
    17761 tgcatttaaa tctatcacta tattatttat attgattttt tcactgatca tttataagcc
    17821 ttctttcaat atataaacta atttttcaaa cgacgaactc gtttttaaat ttgctaataa
    17881 ctcatgattc gaagcgattt cattgaatgt ttcataaatt ttattcaatt caggttttac
    17941 tttttcatta agtgcaaaaa agaaaacaga atgaacaagt gtcccatccc aatcaattcc
    18001 ttctggatta acgtagacac agattactgg cttttttata aaatctactt cgatatgcgg
    18061 catagcaaaa tcattcatcg ctgttgaaga tagtttttct cttttgattg ctccctctag
    18121 tatcccttct ttcgcgtaat tttcttttac caattttttg gtaattatat ttaaaacgtt
    18181 cacttgatta tcctttattt ggtgggtaaa aaagcaaaat cgtttatcta ataaatttag
    18241 aaaagcatct tctgtttgtt catttaactg tagatactgt tttacttttc tgacatctgc
    18301 ccctaacatc aaaggactaa caacaataac aggaaaattt gtattttcaa tcggaatcgt
    18361 gcttataaca agttcatcag tgacttttct atcttgtagt tcattgatgc ttagtacatc
    18421 ttctatctca accatatctg caaaaccgtt atttattctt tgctctaata attttgatgt
    18481 tccatatccg ctactacaaa ctaatatcac tttcgttttg tcaggtttgt aacgatccaa
    18541 aagtgattga atatggagtg ttacgtaagc gatttcatct tcattaaaat gtatacagaa
    18601 tctctcttcg atttcctcta ataaatctac acttatcata aatgaccttt taaaagaata
    18661 tttgattttg tccgtataag ggttatagat atttactccc aatttgagtc ttttaactgc
    18721 tgcattgaga tgtaatacta gattattgat caattcttca tcaggataaa attcatttaa
    18781 tttaattctt actaactctt gtagctcaac acccatatct aaataggaaa gcttcgtttg
    18841 atctaagtcg tgattcatag catttttttc aatcgaagta atgtgaatgt caacataagc
    18901 ttgttcatct attggcacaa taattgaaaa t
//
LOCUS       DADVXO010000054        18879 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.055, whole genome shotgun sequence.
ACCESSION   DADVXO010000054 DADVXO010000000
VERSION     DADVXO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.055"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(85..1134)
                     /gene="recA"
                     /locus_tag="KB950_001517"
     CDS             complement(85..1134)
                     /gene="recA"
                     /locus_tag="KB950_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBB6857739.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1243..2484)
                     /locus_tag="KB950_001518"
     CDS             complement(1243..2484)
                     /locus_tag="KB950_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBB6857740.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2724..3302)
                     /gene="pgsA"
                     /locus_tag="KB950_001519"
     CDS             complement(2724..3302)
                     /gene="pgsA"
                     /locus_tag="KB950_001519"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBB6857741.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3511..4434)
                     /locus_tag="KB950_001520"
     CDS             complement(3511..4434)
                     /locus_tag="KB950_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857742.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5173..6459)
                     /locus_tag="KB950_001521"
     CDS             complement(5173..6459)
                     /locus_tag="KB950_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB6857743.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6452..7720)
                     /locus_tag="KB950_001522"
     CDS             complement(6452..7720)
                     /locus_tag="KB950_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB6857744.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7972..8433
                     /gene="mscL"
                     /locus_tag="KB950_001523"
     CDS             7972..8433
                     /gene="mscL"
                     /locus_tag="KB950_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBB6857745.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8492..8938)
                     /locus_tag="KB950_001524"
     CDS             complement(8492..8938)
                     /locus_tag="KB950_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857746.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9031..9276)
                     /locus_tag="KB950_001525"
     CDS             complement(9031..9276)
                     /locus_tag="KB950_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBB6857747.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9391..9969)
                     /locus_tag="KB950_001526"
     CDS             complement(9391..9969)
                     /locus_tag="KB950_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6857748.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10046..10576)
                     /locus_tag="KB950_001527"
     CDS             complement(10046..10576)
                     /locus_tag="KB950_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBB6857749.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10580..11440)
                     /gene="rlmB"
                     /locus_tag="KB950_001528"
     CDS             complement(10580..11440)
                     /gene="rlmB"
                     /locus_tag="KB950_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBB6857750.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11433..11828)
                     /locus_tag="KB950_001529"
     CDS             complement(11433..11828)
                     /locus_tag="KB950_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBB6857751.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11825..13237)
                     /locus_tag="KB950_001530"
     CDS             complement(11825..13237)
                     /locus_tag="KB950_001530"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBB6857752.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13234..13770)
                     /gene="cysE"
                     /locus_tag="KB950_001531"
     CDS             complement(13234..13770)
                     /gene="cysE"
                     /locus_tag="KB950_001531"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBB6857753.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13856..14095)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14092..15552)
                     /locus_tag="KB950_001532"
     CDS             complement(14092..15552)
                     /locus_tag="KB950_001532"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBB6857754.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15722..16879)
                     /locus_tag="KB950_001533"
     CDS             complement(15722..16879)
                     /locus_tag="KB950_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBB6857755.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16913..18283)
                     /gene="radA"
                     /locus_tag="KB950_001534"
     CDS             complement(16913..18283)
                     /gene="radA"
                     /locus_tag="KB950_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBB6857756.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18346..18873)
                     /locus_tag="KB950_001535"
     CDS             complement(18346..18873)
                     /locus_tag="KB950_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBB6857757.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4988 a         4166 c         3390 g         6335 t
ORIGIN      
        1 cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc
       61 agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt
      121 ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga
      181 aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg
      241 accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc
      301 tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac
      361 ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt
      421 accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt
      481 tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc
      541 tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc
      601 agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc
      661 catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat
      721 atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc
      781 aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc
      841 taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat
      901 agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg
      961 tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat
     1021 tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct
     1081 cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat
     1141 aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat atctttttta
     1201 gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg
     1261 ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct
     1321 ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt
     1381 tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta
     1441 atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac
     1501 tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag
     1561 cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc
     1621 ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt
     1681 cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc
     1741 ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac
     1801 atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat
     1861 cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc
     1921 aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc
     1981 tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag
     2041 aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt
     2101 tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt
     2161 tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag
     2221 agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa
     2281 tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa
     2341 acgctgctga ttatctccta caactgtttg ataataaact tcgataccta gatcagctag
     2401 ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc
     2461 gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt
     2521 ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc
     2581 ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata
     2641 aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc
     2701 acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg
     2761 aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct
     2821 actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc
     2881 atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg
     2941 acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc
     3001 caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta
     3061 tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt
     3121 tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt
     3181 gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca
     3241 accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc
     3301 acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat
     3361 tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg
     3421 aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa
     3481 gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac
     3541 attttttgga ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt
     3601 gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa
     3661 cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac
     3721 tagcacacct aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc
     3781 gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt
     3841 cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct
     3901 ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc
     3961 tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat
     4021 cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg
     4081 cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc
     4141 agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc
     4201 ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa
     4261 ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg
     4321 ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat
     4381 ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg
     4441 ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt
     4501 tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat
     4561 ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga
     4621 cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa
     4681 atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac
     4741 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
     4801 aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
     4861 ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata
     4921 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     4981 tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag
     5041 agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa
     5101 agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca
     5161 taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa
     5221 tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc
     5281 aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga
     5341 atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc
     5401 tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt
     5461 ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa
     5521 ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga
     5581 agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc
     5641 tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt
     5701 aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg
     5761 tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat
     5821 caatttctca ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa
     5881 cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc
     5941 cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat
     6001 ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt
     6061 gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt
     6121 cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc
     6181 attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa
     6241 caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc
     6301 atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt
     6361 gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata
     6421 aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa
     6481 agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc
     6541 tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca
     6601 aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca
     6661 gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta
     6721 attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga
     6781 cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt
     6841 tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga
     6901 ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca
     6961 gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt
     7021 gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg
     7081 cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg
     7141 ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga
     7201 ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg
     7261 cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt
     7321 tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata
     7381 aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac
     7441 cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata
     7501 atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat
     7561 aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt
     7621 gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta
     7681 agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac
     7741 agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc
     7801 ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt
     7861 atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta
     7921 ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa
     7981 gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt
     8041 ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata
     8101 ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt
     8161 gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt
     8221 acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag
     8281 aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat
     8341 cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta
     8401 gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga
     8461 ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt
     8521 cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact
     8581 cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt
     8641 tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt
     8701 cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt
     8761 aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt
     8821 catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga
     8881 cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta
     8941 agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg
     9001 aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc
     9061 aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc
     9121 aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc
     9181 tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc
     9241 taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta
     9301 taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat
     9361 aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt
     9421 ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa
     9481 acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc
     9541 tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat
     9601 tgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt
     9661 ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa
     9721 cagagaatag atttgattgc ttataacgct tttataataa cttccaaagc taacaccttt
     9781 gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc
     9841 ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata
     9901 tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc
     9961 ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat
    10021 tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt
    10081 gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat
    10141 gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt
    10201 cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc
    10261 ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat
    10321 aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat
    10381 tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat
    10441 atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct
    10501 gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca
    10561 acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca
    10621 aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca
    10681 attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta
    10741 aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa
    10801 atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg
    10861 caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa
    10921 ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa
    10981 tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt
    11041 ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg
    11101 caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa
    11161 cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc
    11221 ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg
    11281 cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg
    11341 actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc
    11401 cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga
    11461 atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc
    11521 atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc
    11581 gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga
    11641 tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt
    11701 gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca
    11761 tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa
    11821 tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt
    11881 tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt
    11941 gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata
    12001 aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg
    12061 cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg
    12121 tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat
    12181 tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc
    12241 ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca
    12301 tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca
    12361 agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt
    12421 tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag
    12481 cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt
    12541 ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa
    12601 cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct
    12661 cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt
    12721 cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt
    12781 tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat
    12841 agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc
    12901 ttggatgcag tgtagcaggt tcgacattca acgctttcgt atcttcttca aatgctcgga
    12961 tgaaacgatc agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt
    13021 tatcgtctac atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat
    13081 agcgtcgaat tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat
    13141 acacagtcgg tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt
    13201 ctttttttct ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc
    13261 tttgattcga acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt
    13321 caacacaacg gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat
    13381 ctgcgcattc gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc
    13441 ttttcctgtg ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc
    13501 ggtttcgcca atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc
    13561 tcctggatgg atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa
    13621 taaaaagagc cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa
    13681 accagggtat gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc
    13741 tgcatcaatc gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat
    13801 gtaggaaatc atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa
    13861 agacgctttc cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac
    13921 gcctgttata cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg
    13981 tttcgccaag accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc
    14041 cggtatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag
    14101 tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa
    14161 ttctggacca tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc
    14221 ttttacgcca gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac
    14281 tacttccatt tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc
    14341 agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga
    14401 cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt
    14461 cacccattcg attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata
    14521 aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac
    14581 ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa
    14641 cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa
    14701 taatgcaata aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat
    14761 aaattgcaag attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat
    14821 caatgtcata tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg
    14881 ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg
    14941 atcatcgacc gcaacagcaa aattataagt tggcatacca tcgcgttttt ggatgacaaa
    15001 gtcgccaccg atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc
    15061 gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt
    15121 ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc
    15181 tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta
    15241 tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga
    15301 ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa
    15361 ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt
    15421 atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa
    15481 tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg
    15541 tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc
    15601 gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt
    15661 tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt
    15721 tttattttga ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg
    15781 catgtgcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa
    15841 cgacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt
    15901 catcaaggta ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca
    15961 tcgtttcacc agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa
    16021 caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac
    16081 catctaaaag cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt
    16141 ctccatcgta catttcgaca gatataccct cttctttttg caaggcattt aggatatcta
    16201 atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt
    16261 cgtaaagaac aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat
    16321 cataaatccg accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt
    16381 ctcctgattt tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt
    16441 ttttgcttcg tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca
    16501 atcccagata gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa
    16561 tttccatatt gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac
    16621 cgatcgcacc gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt
    16681 tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc
    16741 ctaacaggct gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct
    16801 gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag
    16861 taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga
    16921 acacacgctt caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg
    16981 cttcccagtt acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga
    17041 cttctttgac tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt
    17101 caccgatgaa acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga
    17161 cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt
    17221 aagcatcttg attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag
    17281 atacccgatt gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg
    17341 tgactaatgc ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag
    17401 cagagccagt tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt
    17461 cttccaatcc atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt
    17521 tttttactgc tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg
    17581 tatccaccat atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac
    17641 cgacgataaa aatggctatc ccatttgttt tagcggtttt gagtagttct gcagtggttt
    17701 ctcttacttg gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga
    17761 tggaatcaat gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca
    17821 tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta
    17881 attttatctg ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg
    17941 ctaattgctg agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa
    18001 tcaagaccat agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt
    18061 ttgttttgat ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt
    18121 tttctcctgt caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga
    18181 ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc
    18241 cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta
    18301 tcctaatcat gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga
    18361 accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa
    18421 aaagatccct tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt
    18481 ggtgaattgg aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat
    18541 cacacctact ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc
    18601 aagctgcaaa aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg
    18661 tttcaattgt ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa
    18721 cccctttttc cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa
    18781 atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata
    18841 tttagaaata acttcaaatc ctctttttct caccggttt
//
LOCUS       DADVXO010000055        18124 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.056, whole genome shotgun sequence.
ACCESSION   DADVXO010000055 DADVXO010000000
VERSION     DADVXO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.056"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(30..1538)
                     /locus_tag="KB950_001536"
     CDS             complement(30..1538)
                     /locus_tag="KB950_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBB6857758.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1697..1888
                     /locus_tag="KB950_001537"
     CDS             1697..1888
                     /locus_tag="KB950_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857759.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2091..3008
                     /locus_tag="KB950_001538"
     CDS             2091..3008
                     /locus_tag="KB950_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBB6857760.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3001..4074
                     /locus_tag="KB950_001539"
     CDS             3001..4074
                     /locus_tag="KB950_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857761.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4222..4740
                     /locus_tag="KB950_001540"
     CDS             4222..4740
                     /locus_tag="KB950_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857762.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4721..5113
                     /locus_tag="KB950_001541"
     CDS             4721..5113
                     /locus_tag="KB950_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBB6857763.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5189..6124
                     /locus_tag="KB950_001542"
     CDS             5189..6124
                     /locus_tag="KB950_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBB6857764.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6257..7036
                     /locus_tag="KB950_001543"
     CDS             6257..7036
                     /locus_tag="KB950_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBB6857765.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7029..7346
                     /locus_tag="KB950_001544"
     CDS             7029..7346
                     /locus_tag="KB950_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBB6857766.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7590..9200
                     /locus_tag="KB950_001545"
     CDS             7590..9200
                     /locus_tag="KB950_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6857767.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9357..10205
                     /locus_tag="KB950_001546"
     CDS             9357..10205
                     /locus_tag="KB950_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6857768.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10436..10915
                     /locus_tag="KB950_001547"
     CDS             10436..10915
                     /locus_tag="KB950_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857769.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10948..13176
                     /locus_tag="KB950_001548"
     CDS             10948..13176
                     /locus_tag="KB950_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6857770.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13305..14948
                     /locus_tag="KB950_001549"
     CDS             13305..14948
                     /locus_tag="KB950_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBB6857771.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15021..15566)
                     /locus_tag="KB950_001550"
     CDS             complement(15021..15566)
                     /locus_tag="KB950_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBB6857772.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15589..16074)
                     /locus_tag="KB950_001551"
     CDS             complement(15589..16074)
                     /locus_tag="KB950_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6857773.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16064..16876)
                     /locus_tag="KB950_001552"
     CDS             complement(16064..16876)
                     /locus_tag="KB950_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBB6857774.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17015..>18124)
                     /locus_tag="KB950_001553"
     CDS             complement(17015..>18124)
                     /locus_tag="KB950_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBB6857775.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5885 a         3301 c         3628 g         5310 t
ORIGIN      
        1 cgttttcaac atttataagt aaactttttc tattggtaat atagattttc ggaagggtag
       61 acaggtgcgc aggtaatatc gattcctagt tttttcaatg tttgttcatc atttttattg
      121 accatcacag tagagtgggc ttgtgctccc tcaagttcag ctagtttatc ataagcaact
      181 tgagcgattg gattcgttac tgcactgatt gataaagcga tcaagacttc attgatgctt
      241 agtgcatcca aagaggaatg aagtgtcttt gatttcaatc cttgtattgt ttcaagaact
      301 aatggtgaca ataaaggaat gtcatctgcg atatgggcta aatgttttac tgcatttaag
      361 ataactgccg ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat gacttcatct
      421 ggtaattcga ttgcaatcac agcagaagga tcttttgtag gactttgcgc ttgaatctta
      481 tctgcatagt catgggcagg gactaccacg tttcggtctt caggacgtaa accgacttct
      541 tccataatca gtttgattcg attaactgct tcttcgtcga tcaatccttt tttgaagtcg
      601 cattctgttt ggaaataacg acggatgatt tcttgtttag aggcttcttg gacaacggta
      661 tcatccacga tcccaaatcc tacacgattc acacccatgt cagtaggaga ttgataaaca
      721 gattctttac cagtaatttt ttcaataata cgtttgatta caggaaatgt ctcaacatcc
      781 cgattatagt tcacagctac tttattgtag gcatcaaagt ggaaggaatc gatcatattg
      841 acatctttta gatcgactgt ggctgcttca taagcaatgt tcagcggatg tttcaacgga
      901 acattccata ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt
      961 tcatgataca gttggttcaa ggaagtagcg agctttccac ttcctgggcc aggagctgtc
     1021 actacaacga ttggtttagt tgtttcaata tactcatttt ttccaaaacc atcttctgag
     1081 acgatttttt ctacgttgac tggatagtct tcgatttctg agtgtttata gaccttgatg
     1141 tttcgtcttt ctagtttatt gataaagact ttcgtggcag gctgtccact atagcgagtg
     1201 ataacgacac tgttgattaa taagccataa ccacgtaatt catcgattaa ccgcaagata
     1261 tccatatcat aagtaatccc ataatcacca cggatcttat tacgttcaat atctccagca
     1321 tagacgcaaa tcagtatttc agcttgatct ttcaattttt gcagcaattt gattttcgca
     1381 tcttcttcaa atcctggaag tacacgtttc gcatgcttgt cgtcaactag cttcccacca
     1441 aattctaaat ataatttgtc atagtggttc actcgttcca aaatatacgc tgactgctct
     1501 tcaatatatt tttctgaatc aaaaccaatt tttttcacta ggttccctcc ttaagtacta
     1561 aagcattttt tcattttttt gaggattcgt caactggaag agcgaaattc ctcaaaaaaa
     1621 caaagaaaat ggtttaaacc aacttgtcta gatagttatt cattggtatg atagtttttg
     1681 ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa aacgcatata aacactatat
     1741 ccgttcgatt tttaccggtt atgaaagaat gcttgaagat cccgaattgg aacaattgac
     1801 gtttacttat ctaaacgaag aaacgcagct aactcgtgat gagcatcagc gcattcattt
     1861 cacaacaaga gatctgccta gtaaataaaa aataagataa aggtcatcgg attcgattct
     1921 gtcctatcag atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag
     1981 cctgttttat ggagcattgc ccgctctttt ttataacaat tttttacaga aaatggtact
     2041 attgaagtat taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc
     2101 cagatacaaa tgcaaattat gagccagacg aactagagga gacgaatgcg gatcaggata
     2161 caggacaaga atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc
     2221 aattattaga agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga
     2281 tctaccgttt atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc
     2341 acgagatcga tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt
     2401 ttatggaaca agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt
     2461 accatgatcg gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta
     2521 agatcgatga agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa
     2581 tcaatgagct agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac
     2641 aaataaaaga aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc
     2701 gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg
     2761 aaaaattagg ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag
     2821 cattgaagca agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa
     2881 ggcaaatcag acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct
     2941 tgatgaatta tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt
     3001 atgaatgaac acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa
     3061 gaaacgaaca cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc
     3121 cctatgctag aggaaacaaa aatacacgaa aaagaaaaag taactccgac gaatacgaat
     3181 actgcagaaa acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa
     3241 gagcaagatc atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat
     3301 ttacgtatga gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat
     3361 cgttttatat tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac
     3421 ttcacgtatg aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct
     3481 tatgacacat ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa
     3541 ttagtctttt cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt
     3601 ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct
     3661 ttgatccgac actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat
     3721 cacaaattga atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca
     3781 ttactagaca atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat
     3841 gaagtattgg atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag
     3901 atcaacgaaa ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca
     3961 aacgaaaaag atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt
     4021 gatttcttta tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat
     4081 aagttggatt accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc
     4141 cagaataatt gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat
     4201 gaaggaggga tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct
     4261 gctgcttgtg tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact
     4321 gctatttttt tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt
     4381 ctttctattg cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg
     4441 atcagctata ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa
     4501 aggaatctat ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt
     4561 atgaccgttt ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa
     4621 ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg
     4681 ccagtcgtca aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag
     4741 tttgcttgca atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca
     4801 atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca
     4861 agaaaatggt caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg atgatttact
     4921 gagtattttg aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga
     4981 agggcactca caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag
     5041 tgctactact ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt
     5101 gtctaaacta taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga
     5161 gttcagttga tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc
     5221 cgtaattgct gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc
     5281 aatcagtggc agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa
     5341 tcagattttg ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca
     5401 atttggtgaa ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt
     5461 cggcggtggg gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga
     5521 agtgggtaat ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag
     5581 ttcgttacgc gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat
     5641 gcgctttttg cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg
     5701 tgctgtaatc ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat
     5761 tgctgcattt gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat
     5821 tttcctagca aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt
     5881 atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac
     5941 agttcattta agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa
     6001 agagaatgaa gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc
     6061 tgacaagaag atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa
     6121 ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa atataatctt gtttttaaat
     6181 aaaacggcta caatttagcg aatttatgtt acaatgagtc cgctgtttat ttgttgtaat
     6241 taaaggagat attttaatga acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc
     6301 agtgatgcca ctttgtataa gctatatacc agtaggatta gcttgcggag tattgttgca
     6361 aaaagttgga tttgatcctc tcttatcagg actgttatcg attctggtct tttccggtgg
     6421 cgcgcagttt ttagttgcat ctatgcttac gacgcaagca tcatttgcaa cgacattatt
     6481 gatggttttc tttttagaat tacgctatac attattagga gcaagtcttg ccggttttat
     6541 gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa tcactgaatg atgaaaacta
     6601 tgcggtgaac tacttaaagt tttcaactga cccttcctgg aataaaagaa aggctttata
     6661 tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat atgatcggta attttttcgg
     6721 ctcggtgatt cgagtagatg ctgaccttgt ccattttgct ttgacggcca tgttcatttt
     6781 catgtttatc atgcagatga aaagcgcact actgatcttt acaggtttat tctcaggcgt
     6841 attaggggtc gtattcatgg tattattcca aaatactttc ggattgattg cagcaacagt
     6901 cattgcttca tttgccggat ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa
     6961 actaagagac cgcggtaaag atgtcaatga gccgctcttc aacttcccgg aacaggaggc
     7021 caatcaccat gactaaacta tatttgctac ttttagttgc ttgtctgttc atcgtggcat
     7081 atatcccgcg cttgttcccg atgctttatt ttactcatcg aaaagtgcca tcctggttca
     7141 gtgactggat gaaatatgta ccggtggcat tatttgcggc gttagctttt aaagatgttt
     7201 tcattactca tgaacatctg gatattgctt ggaatatcaa aatagctgca atgattctag
     7261 tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc agttttgaca ggacttgcgt
     7321 ctgtcttttt gctgtctatg ctttaaactg ttttatttta ttaaaaacag agattgcgac
     7381 ataagttgtt caaactgatc tatccgaact gcatttgcaa cagtcaattc tggatgcaag
     7441 tttattcgta gggataaaaa agattagtgt tggcttatga tgcaagctct tttttatttt
     7501 aggaattaga gagggatttg tccttctatg attttttgct ataataggga agatgtttat
     7561 tttatgaaag aatagttgag ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt
     7621 ggctaacctt tgctaatctt ctatgtaaag ttttaggggt agtctatttg attccctggc
     7681 taagtatgat ggggaacaat caagatggta tgcttgctac gacattgtat aatgttggct
     7741 atcttcctta tggattgttc ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa
     7801 aaaaagtagc tgtagccaca aaagaaggag ataacgctgc ttgtcggctg atttttagaa
     7861 gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc tgcactgatg tatttatttg
     7921 ctcctttact agccggaatc agtccgattg ctaacgttaa taatgggata ctagctatca
     7981 gaagtttatg cccatctttg attgctatcc caattcttag tgcaatgaga ggatatttcc
     8041 aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat tatcgaacag gctgtgcgag
     8101 tgatcgttat cttggctggt acttattact tgcgcgtatt gactgacgga accatattag
     8161 aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg attatccata
     8221 tgtatatcgt cggacgcaga aaagacttct tcgaacttaa agattttttg atttccagcc
     8281 gttatttcga aagagaaaac cgatctgcct ctgtctctat cattcgggaa acactaccgt
     8341 ttatttttgt cggctcagtc attactatcg ttcaaatgat cgaccaagta acgatgaaac
     8401 cactgcttca ctttttccgt ccagagatcg cagatcagca actggaattc ttattcagcc
     8461 gtgcttctgt caatccaaat aaattgacat tgatcctgat ctccatggta ggaacagtag
     8521 ctataagcag cttgccaata ttaagcacga tgaagaaaaa agatcggata caaatcgaaa
     8581 aaacagtagg agatagtttt tcgattgcgt tattgattct tcttccttca ttgactggaa
     8641 tgtctttatt agctggacca ctatacacat tgttttttgg ctatgatcct gaaagtgtcg
     8701 gatatttcca aatggctttg ctagcttctt tgtttttctc tttattcacg atcctatcta
     8761 cgatgcttca atctttgaat caccatcgtt ttgcgatccg attaactgta gaagcaatta
     8821 tcctgaaagt catcttccaa gtaatcggat taggattagt tggcggatac gggatgagct
     8881 tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag
     8941 aataccgaat agcaccttta gcaaagatca gcaatttttt cttaaaaact ttccgctcca
     9001 ccctgattat gcttgcagta tgttttgccg ttttcttttt gctgaatcag cgattgtcaa
     9061 tggaatcaaa aagctatgca atgatttatt gtgtggctgt aggaagtatt ggcggcttag
     9121 tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc
     9181 atagcagaag caaagaatag gttaactaat cgttttttgc tgtcacttga aaaacggcaa
     9241 ctcgtatgat tacgcttgtc gtttttctat tttccaattt tagtttcata tctaatctta
     9301 cttatgatat agttataagg tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga
     9361 aacttgcagt agttacagat agttcggcat atctgccgga acgtatcaag aaccatcccg
     9421 acttattcgt tattccaatc cctttgatta tggatgggaa aatttataac gaaggaatcg
     9481 atatcgaagc ggatgaatat tatggacttt taaaaaacag taaagatttt cctactactt
     9541 cacaacctgt cgtaggggaa gttttgggtt tatatgagga attgaaagag aaagggtatg
     9601 acacgatttt aagtatccat ctttcttcag ggatttctgg ctttgtcaac acattatttg
     9661 cgatgaaaga tgacatcagc ggagtaacgg tcattcctta tgactctaag atcactagta
     9721 tgccgatggg acacatggta gaagctgcct tagacttaaa tgatgaaggt cagtcccttg
     9781 atgaaatact tgcacatatc gatcgaatcc gagataatac ctacgcttat ctgattgtag
     9841 atgatttgaa caatttagta cgaggaggac gtttgacaaa tggtgcagcg ctgattggca
     9901 gcttattaaa aatcaaaccg atcctgacat tttctgaagg gaaaattgtt ttattcgaaa
     9961 aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga aattattggt agaagaaata
    10021 acgaaataca accgccagtc aaactttacg tgatacatgc aaataattta gaagtagcga
    10081 ttgcggagaa agagaaattg cagaaaaaat atccaaatgc agaaatcgaa attggtcatt
    10141 tcggaccagt cattggtaca catttaggtg aaaaagccat cggcattgcc atttcagcgc
    10201 aataattcct tttattcaaa aaacatacca aaaatattcc aacatcatct tggactttct
    10261 acatgttact aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc
    10321 tctttttctt tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg
    10381 agtaaactac taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga
    10441 aataccaaca aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga
    10501 tggaagaaat gcattaaata cggatgcaaa agggctttca ctgggccttg ctcagtggaa
    10561 tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc
    10621 acgacagtcg gaagaattac caatcacacg tattttagat ttagcgatct tagctgcaca
    10681 aggaagttta tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc
    10741 agtcgtagac atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa
    10801 tccaaagatc gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact
    10861 gattggggaa cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac
    10921 agagatgatc aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc
    10981 ttttcacggc tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca
    11041 gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc
    11101 agtaacgtac ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta
    11161 tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca
    11221 gatgttttcg gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta
    11281 ctagaagcta tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat
    11341 tacggcattg atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa
    11401 gctgattatc cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt
    11461 gaaaaaggat atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt
    11521 gtggcactta actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt
    11581 cgtcgccatc cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc
    11641 ccattaaatg aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc
    11701 ttagaagtca tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc
    11761 ttaaagatgg cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa
    11821 ggtgtagcag gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat
    11881 ttactccagt cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg
    11941 gcaattcttg aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg
    12001 agaaaagaaa aatggggtca tcctcaatct gagcatacaa aaggaagaac cattagcgcc
    12061 gaagaagcaa atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa
    12121 aagaaatcgt tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata
    12181 gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa
    12241 aatcaaaaag aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa
    12301 gcacatgata aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa
    12361 acgattcatg aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg
    12421 gaagcacgtg agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt
    12481 ttagagttac tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag
    12541 cggttcaatg caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg
    12601 ccacatgagc aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata
    12661 cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag
    12721 aaagtcgata tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag
    12781 acttctttgt tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta
    12841 gtcactatga aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa
    12901 aaattaaaca gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca
    12961 aaagcgttgc cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa
    13021 gaatatgaac gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa
    13081 atcaagtcga tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc
    13141 aagcaaatga ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac
    13201 ttagcagatt cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata
    13261 gaatcggtta taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa
    13321 aatggtggaa agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg
    13381 gcgatggtca aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg
    13441 gtatcggctt tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg
    13501 acgtaagtga ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat
    13561 tgctaaaaga agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata
    13621 gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg
    13681 attattacat ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt
    13741 tcggcggttc tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg
    13801 caaaagaaca accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata
    13861 tgatccgatg gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg
    13921 taaaaaaaga tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg
    13981 tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca
    14041 tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag
    14101 acggttattt cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag
    14161 aagggcaatt agatgtcctt aaattgaaac ttgcattaag cgcttggcaa acttcattgg
    14221 atggtatcgg atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag
    14281 tgtttggaga tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt
    14341 atttcttttt gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata
    14401 tgccctttga atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc
    14461 tgcttgcgga aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag
    14521 acaacagcag gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg
    14581 aaccttggct gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg
    14641 aaccagagtc ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag
    14701 gtctgatata cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg
    14761 aaagatattt agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg
    14821 actatgaact tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta
    14881 gcggagaatt tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa
    14941 acaagtaaaa cttcctcgtt aaagttcatt agttggactt tgcgaggaag tttttcagat
    15001 tgctctgtca attactcatt tcaataattc gctctaggac caccgatata tttaggacga
    15061 gagcgaaggg ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact
    15121 tggacatgtt tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca
    15181 actacgtgtt ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca
    15241 cctccagcat gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact
    15301 gttcgttcta ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt
    15361 tctttaggat ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg
    15421 gaattttttc caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga
    15481 tcaccttttt tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct
    15541 tttaattctt tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt
    15601 ccgtgcttga atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag
    15661 attttggaag atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca
    15721 accggcgata ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca
    15781 catacgagta gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac
    15841 taatgaaaaa actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga
    15901 agcaccaaca actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc
    15961 ctcgcataat gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag
    16021 ggcaactgtc aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca
    16081 ttcctccttt ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc
    16141 cattagaaat acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg
    16201 cccctaacac aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg
    16261 ttgtagtgtc gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat
    16321 ccacagcttc atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg
    16381 aagcagtgag ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac
    16441 cagaaagtaa agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt
    16501 cttcgatgag ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca
    16561 aaacagggtc taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca
    16621 tgattgcttc acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat
    16681 caatcgaaac taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct
    16741 cattaatatc tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct
    16801 caaaagtttt taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg
    16861 tcaagattct tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa
    16921 agaaaaacag ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag
    16981 aaagaccaaa atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt
    17041 tctcgatttg aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac
    17101 ggatgatagg gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat
    17161 ccatttcttt atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta
    17221 caatcactac ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg
    17281 tactattgat cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct
    17341 tatccataaa accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc
    17401 gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat
    17461 ctgtgttttc aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt
    17521 ccagtccaac gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct
    17581 taagaatcat tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg
    17641 ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt
    17701 gatagtatcg gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa
    17761 caaagttatt cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa
    17821 cttttgaact ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag
    17881 agataccaat attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt
    17941 ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag
    18001 aatgacaaat aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt
    18061 catttttgtc taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa
    18121 tagg
//
LOCUS       DADVXO010000056        18049 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.057, whole genome shotgun sequence.
ACCESSION   DADVXO010000056 DADVXO010000000
VERSION     DADVXO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.057"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(57..1793)
                     /locus_tag="KB950_001554"
     CDS             complement(57..1793)
                     /locus_tag="KB950_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBB6857776.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1790..2494)
                     /locus_tag="KB950_001555"
     CDS             complement(1790..2494)
                     /locus_tag="KB950_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6857777.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2625..3128
                     /locus_tag="KB950_001556"
     CDS             2625..3128
                     /locus_tag="KB950_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBB6857778.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3088..3426
                     /locus_tag="KB950_001557"
     CDS             3088..3426
                     /locus_tag="KB950_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBB6857779.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3447..4865
                     /gene="ffh"
                     /locus_tag="KB950_001558"
     CDS             3447..4865
                     /gene="ffh"
                     /locus_tag="KB950_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBB6857780.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            4977..5555
                     /locus_tag="KB950_001559"
     CDS             4977..5555
                     /locus_tag="KB950_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBB6857781.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5559..6080
                     /locus_tag="KB950_001560"
     CDS             5559..6080
                     /locus_tag="KB950_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6857782.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6159..6713)
                     /locus_tag="KB950_001561"
     CDS             complement(6159..6713)
                     /locus_tag="KB950_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBB6857783.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6966..7241
                     /gene="rpsP"
                     /locus_tag="KB950_001562"
     CDS             6966..7241
                     /gene="rpsP"
                     /locus_tag="KB950_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBB6857784.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7253..7504
                     /locus_tag="KB950_001563"
     CDS             7253..7504
                     /locus_tag="KB950_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBB6857785.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7629..8420
                     /locus_tag="KB950_001564"
     CDS             7629..8420
                     /locus_tag="KB950_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857786.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8590..9111
                     /gene="rimM"
                     /locus_tag="KB950_001565"
     CDS             8590..9111
                     /gene="rimM"
                     /locus_tag="KB950_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBB6857787.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9101..9862
                     /gene="trmD"
                     /locus_tag="KB950_001566"
     CDS             9101..9862
                     /gene="trmD"
                     /locus_tag="KB950_001566"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBB6857788.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            9998..10345
                     /gene="rplS"
                     /locus_tag="KB950_001567"
     CDS             9998..10345
                     /gene="rplS"
                     /locus_tag="KB950_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBB6857789.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            10718..11380
                     /locus_tag="KB950_001568"
     CDS             10718..11380
                     /locus_tag="KB950_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857790.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            11459..14686
                     /gene="fss3"
                     /locus_tag="KB950_001569"
     CDS             11459..14686
                     /gene="fss3"
                     /locus_tag="KB950_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBB6857791.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14800..15114
                     /locus_tag="KB950_001570"
     CDS             14800..15114
                     /locus_tag="KB950_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6857792.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            15127..15501
                     /locus_tag="KB950_001571"
     CDS             15127..15501
                     /locus_tag="KB950_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6857793.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            15511..15855
                     /locus_tag="KB950_001572"
     CDS             15511..15855
                     /locus_tag="KB950_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBB6857794.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            15926..17278
                     /locus_tag="KB950_001573"
     CDS             15926..17278
                     /locus_tag="KB950_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBB6857795.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            17338..>18049
                     /locus_tag="KB950_001574"
     CDS             17338..>18049
                     /locus_tag="KB950_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBB6857796.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGML"
BASE COUNT         6029 a         3231 c         3842 g         4947 t
ORIGIN      
        1 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
       61 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
      121 gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
      181 gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
      241 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
      301 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aattagattt
      361 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
      421 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
      481 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
      541 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
      601 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
      661 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
      721 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
      781 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
      841 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
      901 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
      961 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     1021 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga
     1081 tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga
     1141 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     1201 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     1261 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     1321 ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     1381 ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg
     1441 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     1501 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     1561 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
     1621 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
     1681 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
     1741 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg
     1801 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat
     1861 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
     1921 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
     1981 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
     2041 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
     2101 gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac
     2161 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
     2221 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
     2281 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
     2341 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
     2401 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     2461 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     2521 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     2581 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     2641 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     2701 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     2761 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     2821 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     2881 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     2941 gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg
     3001 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     3061 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     3121 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     3181 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     3241 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
     3301 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     3361 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     3421 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     3481 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     3541 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     3601 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     3661 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     3721 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     3781 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     3841 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     3901 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     3961 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     4021 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     4081 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     4141 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     4201 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     4261 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     4321 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     4381 acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     4441 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     4501 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     4561 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     4621 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
     4681 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
     4741 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
     4801 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
     4861 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
     4921 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
     4981 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
     5041 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
     5101 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
     5161 tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
     5221 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
     5281 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
     5341 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
     5401 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
     5461 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
     5521 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
     5581 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
     5641 acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca
     5701 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
     5761 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
     5821 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
     5881 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
     5941 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
     6001 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
     6061 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
     6121 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
     6181 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
     6241 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
     6301 ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc
     6361 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
     6421 ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
     6481 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
     6541 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
     6601 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
     6661 ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt
     6721 ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa
     6781 acacttactg acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc
     6841 taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt
     6901 tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat
     6961 aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc
     7021 gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta
     7081 cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact
     7141 ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg
     7201 ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga
     7261 tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa
     7321 gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga
     7381 tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta
     7441 cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa
     7501 gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt
     7561 gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg
     7621 aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta
     7681 gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta
     7741 ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct
     7801 caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca
     7861 tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc
     7921 aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat
     7981 tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag
     8041 aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag
     8101 gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag
     8161 aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat cccttactat
     8221 tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg
     8281 gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag
     8341 aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag
     8401 tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt
     8461 cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta
     8521 aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg
     8581 ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa
     8641 ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg
     8701 gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc
     8761 aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa
     8821 aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat
     8881 gaattctatt accatgagat catcggtctg catgtattag aagatggaac tgagttaggt
     8941 aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa
     9001 aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct
     9061 ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac
     9121 tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga
     9181 taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca
     9241 gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat
     9301 ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct
     9361 tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga
     9421 acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt
     9481 gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt
     9541 catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca
     9601 aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata
     9661 taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg
     9721 gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact
     9781 tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa
     9841 aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat
     9901 gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa
     9961 agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt
    10021 aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt
    10081 tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat
    10141 caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt
    10201 gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg
    10261 ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc
    10321 tcgtatcaaa gaaatcagaa gataaacacg gaaaacttcc ttggaaatct gaaatttgtg
    10381 aaaagtcctt gtatatcaag ggctttttct tttttctcta tcacagtttt aagccatttt
    10441 tggctgtttt ggaccatttt tcaatcatat tcccttaaaa attgtcctag attgtccgac
    10501 attgactatc agtgtcgtca aaatagggca aaatttagta gtcagcttga cgacaaatat
    10561 aatacgaaaa ataccaaaac atttaccgaa tttagcaact tttgaaccca agtttctcaa
    10621 agggtcagct attagcaaaa aagacaaaac atgtactttt ctactaaaaa gttgatgtga
    10681 taggcttcta tctaaataac tattaggagt ttttatcgtg aagcaaaatg atggacgtat
    10741 catttggaaa aatcaaagtg agttaaaact gattttaaca attaatgaat tcatcgaaaa
    10801 acacggaatt acatctagcc gtcaatacca gaaaaagcta tcagaaaatc caaattctgc
    10861 accaagtatg tggttcatta acaaaaaata tggatcatgg gagaatttat tgattagtat
    10921 aggacgagaa aatactgggt atggaaaatg ggctagaatg tcagaacaag aattattgga
    10981 aatagtagaa gcctttatta agtgcgagaa gataacctct caacgaatgt acgaacaaaa
    11041 atcagtaggg aagaatattc cttcacttag taccattaaa aagatgttag gagatatacg
    11101 tccacttttt aaagaaaaaa atgatggatc tcgatttact gatttcgaac tgctgttaga
    11161 actaaaaaat gaaataatcc gattaaaact acaggatgat ttatcaatga caaagtttcg
    11221 aaaactagtt caatccccaa aactaccatc agttgataca attatgaaaa gaacgaacaa
    11281 aaattgggag gaattgatgg cagagattgg atttgattat cgaagaatca aaatttataa
    11341 gcagagaaac aacctatcca agacaaagaa aactaaatag tatcactcta aatcagacgt
    11401 tcaaaattaa aatttggacg tcttttttat tacccaaaaa cgaaaggaga tttattttat
    11461 gaaaaagcca agatttaaga attggcgttt gctcgcaaca ctgagtctat tatgccaaac
    11521 aattggtgga tcgcttggcc caacgattgc ctttgctgat gaaattactc atccacaaac
    11581 ggtaactgtg gaattggatt tggctcacca gtatgctgtc gaaggtacat ttagcgatgg
    11641 tcgtcccatg tcagaagtta ccgttccgca ctatgcagtt tacaatggtg tgaagcaaga
    11701 tattttctgt attgagccag gggtgccgat ttacaatgag ttcactcctg gttatgagaa
    11761 aaatccattg cctgatatgt cggagaaagc gaaattagtt tccgttttat ggaaggaagc
    11821 tggtacggat gtggatacac atattgtggc ccagaagatg atttggcaag aagttaatgg
    11881 ctacacgctt cactcaatta aacgttctga tggtagtgca gtaaatatcg cagccattga
    11941 gtcaaaaatc aatcaagcca ttgcggatta tcaaaagaaa ccaagcttcc ataactccac
    12001 agtgaaaacc gtgttaggtc aatcgactac tgtgacagat acgaacggct tgaatttatc
    12061 ggagtttgat gaagttgtcg agaatatcgc aaatattgat tatcgtgtga acggcaatca
    12121 actagtgatt actccaaatg ccaattcaaa agagagtggt gaattaaccc taaagaagtc
    12181 agctaataca ggaacaccgg tagcttataa aatggctggt caacaaactt tgatggcggg
    12241 ggcaattgat aagcccaata cctacacaat caaaattgat gtggaaactg agggttcttt
    12301 gaagattaaa aaagtcgata aagaaacagg aaatgttgta ccgggaaccg ttttccattt
    12361 agactttgga aaaacattac ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac
    12421 attggatgag attcctcatg gcgcgaaagt aaccattact gaaaaatcag tgccagctcc
    12481 ttatacgatt gacactacac caatcaccgc aaccattaaa tctggtgaga ctatttctgt
    12541 gacttcaaaa aatactcgcg aaaaaggtca aatcattttg aataaaatgg gggtggaaac
    12601 aggaactgat ctttggaacg acaaatactc attagccgga aatacgtttg ccattcgcaa
    12661 agacagtcca accggtgaaa ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga
    12721 aacaccaaaa gagattgcca atgcgttgga attgggaacc tattatgtga ccgaaaccaa
    12781 agctagtaat ggctttgtga atacctttaa accagtaaaa gtcgaattga actatgtcaa
    12841 tcaaaccgtg gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga
    12901 aaccactttg acaaaagaag acaaagatac cggtaatgaa actcaaggta aagctgagtt
    12961 taaaggagct gaatatactc tctttactgc aaaagatggt caagcggtca aatggagtga
    13021 ggcttttaaa ccagagttag tgaagggaac gaaagcttct gatgaaacgg tgactttgac
    13081 tttagatgaa aagaatcaag ttgctgttaa acacttagcg attaatgagt attactggca
    13141 agaaaccaaa gcacctgaag gatatacctt ggatgaaaca aagtaccctg tttccatcaa
    13201 aaaggtggat gataacgaaa aaaatgccgt gattacccga gatgttacgg caaaagaaca
    13261 agtcattcgc tttggctttg atttctttaa atttgctgga tcagctgctg ggactgccga
    13321 aactggcttc aatgatctga cctttaaagt atcgccattg gaaggaacca gtgagattac
    13381 aggtgccgaa gatgaagcaa ttacagctta taacgagcaa ctaggttttg atggctatgg
    13441 caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagagg ctccagaagg
    13501 atttcaaaaa attacgccat tagaaatccg ttctactttt aaagaaaata aagcggatta
    13561 cactaagagt gaatacattt ttacgattac cgaaaaagga caaaaacaac caattaaaac
    13621 ggtgaccgtt ccttacgaga aactgacgaa taaagcattt tctgttagct tgaatcgttt
    13681 gatgctctat gatttacctg aagaagaaga tagcttaact tctcttggga cttggaaaga
    13741 cggaggcaaa gaattgacag accttgattc taccgagcta gttgataaat tgagctataa
    13801 cttgcatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggaagcgac
    13861 aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc
    13921 aacgttggcg aataaagaga aaacaggaac ttggaaaatt ttgcataaat taaccgcaga
    13981 acaagttttg gataaaacca tcgtcttatt caactatgtg tatgaaaaca agaaacccta
    14041 tgaagcaggt gatgatccag tagcgaaaga tgctagcttg aacaatcaag cccaaactgt
    14101 caattgtacg gtggaacgcc atgtttccat ccaaacaaaa gcccacctag aagatggttc
    14161 gcaaaccttt actcatgggg atgtggtgga tatgttcgat gatgtgtcga ttacccatga
    14221 tgtactggat ggctcaaaag aagcttttga aacaattctg tatgcattac ttccggataa
    14281 tacgaccaaa gaaatttgga aatctggcaa aattgattat gaagtgaatg acaaagaatt
    14341 cactaaaacc gtacttgcgg aaaaagtgga cacaagtaaa tatccagaag gtacaagttt
    14401 taccttcaaa gaaattaact atggcaaaga cggaaacatc aatgggaaac acaatgagga
    14461 tctaaaagaa aaaactcaaa ctttaacacc taaaaaagtt ccgacaacac caagtgcacc
    14521 agaacaaccg gaaataccaa ctgttccaag tgactctcaa gaatctagtc ctacagtaaa
    14581 gacattccca caaacagggg agaaaaattc taatgttctg ctgtttattg ggtttacctt
    14641 gatctttgcg actgctgggt attatttctg gaatcgccga aactaaggtg atgtgatgaa
    14701 acaactaaat aggaaaaacc aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat
    14761 ttttgtatcc aaaactaaaa atgaaaagag gaaaagaaga tggaattgaa atttgttgtg
    14821 ccagatatgg ccgaaacctt tggaaagata aggtatgccg gcgaaggcga agttttaact
    14881 gaaggatatg ggcggaatac tacagtaatt ggtcgtagtt atcatttgta ttccagtaag
    14941 caacgagccg atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgat
    15001 caagatcaac cactaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag
    15061 aatcgtgggt ttaccgacta cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa
    15121 aagaagatga gattaacaga aggtattgtt gtagattcag gactaacgtt tgggaaatta
    15181 cgattttccg cattacgccg agaagtacgg aagcaaaatg aggatggaac ggttagtaac
    15241 gaggtaaaag aacggactta taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt
    15301 agtatcccag caaatgtacc cttacgcgag tttgcctatg acgcagaggt agagttggtc
    15361 aatccaatca tggatacggt cgccaattat gttttccgag aaggaactac tgtcaattgg
    15421 ttcattaagg ccgatgattt agtcttgaaa cgacaaccag atcagggaaa ttctacaaac
    15481 caaaatgaag gaaagaaata ggtgaagcgt atggaaatta atgttgaaca tattttagat
    15541 tgtctcgatc aatatggaaa aggtgagcta acggaggaac aactcactaa agcattaacc
    15601 tatgatgaaa aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgtc
    15661 gaagaagatt ttgatgtact gaattggtta ggggaagaag agtccttctt tatggttatc
    15721 gaagtaaatg aaagtctatg tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa
    15781 atgccggatg ccattgaagt ttttttaaag caactagtgg aaacgaaaca actccctata
    15841 gcagtaaatg actagcataa ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt
    15901 tcgattggag gtggaaacca taaaaatgta caaagggcat cgtatacggg caggagatca
    15961 gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag
    16021 cgtcttttat tttcaagagc ttagacaatt tgatatttct aagctatcac tttctactat
    16081 agaaatcgtt cgttctataa aagatttaat ctatttgcta ggaagtctcg ttctttcagg
    16141 aagcacgatg ttgctttata tacacttttt tcaggatcat tggcgcagtt tgtggcatcg
    16201 acaaaagcta gcccggatga ttctagaaaa tcattggtac gaagtaaaac aaactcagag
    16261 tgaaggattt ttcaaagacc tcaatagtag tcgaaccaaa gagacaatca gttacttccc
    16321 caaaatctat taccgtatga aggatggttt actttctatt cgcgttcaaa tttcactggg
    16381 aaaatatcaa gatcagcttt taaagctgga aaagaagtta gaaagtggtc tatattgtga
    16441 actagtagaa aaggaactca aagactccta tgtggagtac actttactgt atgatataat
    16501 tgccaatcga attgggatag acgaagtagt ggcagaaagt ggggctttgc gattgatgaa
    16561 aaatcaagta tgggcttatg attctttacc tcatatgtta atcgctggcg gcacaggtgg
    16621 tgggaagact tactttttac tcactatcat cgaggcttta ttaaaatctg atgccgagct
    16681 ttttgttctt gatcccaaaa atgcggactt agccgattta ggtacggtaa tgcctcatgt
    16741 ctattcccaa aaggaggaaa tttctgcttg tgtggaagat ttctatgagc gcatgatgac
    16801 tcgtagcaag gcaatgaaag aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct
    16861 tggacttcca ccaaactttt taatctttga tgaatacgtg gtttatatgg aaatgttaac
    16921 gacgaaggaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca
    16981 atctggtttc tttctgattc tagcttgtca aagaccggat gcgaaatatt taggagacgg
    17041 gattcgcgat caatttaatt tccgggtggc tttgggtcgt atgagtgaac tgggatattc
    17101 catgatgttt ggcgaggttg ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta
    17161 tgtagatacg ggagggagtg tgattagtga gttttatacg ccacttgtac caaaaggata
    17221 tgatttctta agggaaatta gcgacattgg tggattaaac gttaatacgg aaggataaaa
    17281 taatagtatg tagcctagga gtgtcaagaa tcctgggctt tttttacgag gtgatttatg
    17341 acatttttag atttattcgc tgggattggt ggcttttgct taggtatgga acaagcgggt
    17401 caccagtgta tcggcttttg tgaaattgat gattttgctc gccagagtta taaggcaatc
    17461 catgatacaa gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt
    17521 caatctctcg gaccagtgga tatcctttgc ggtggatttc cgtgccaagc tttttcaatt
    17581 gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg
    17641 ttcgccgcta ttctccaacc taagttttta ttccttgaga acgtccgggg attactcaat
    17701 cacgaaggag gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgat
    17761 gtggaatggc aagtgcttaa ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc
    17821 cttatcggac attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggatcgtct
    17881 ccaacatctg atcaaaacat cagaaactta ttaaatatca acccttccaa tcgaggaatg
    17941 gggggacaag tctatgggtc agatgacgta gcgcctacct tgaccggtga tgaaggaatc
    18001 aaaatcgcat tgccggtaga tgatggaatt tctgtagcgg gtatgttac
//
LOCUS       DADVXO010000057        17452 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.059, whole genome shotgun sequence.
ACCESSION   DADVXO010000057 DADVXO010000000
VERSION     DADVXO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.059"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(157..702)
                     /locus_tag="KB950_001575"
     CDS             complement(157..702)
                     /locus_tag="KB950_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6857797.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(714..1406)
                     /locus_tag="KB950_001576"
     CDS             complement(714..1406)
                     /locus_tag="KB950_001576"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBB6857798.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1425..1709)
                     /locus_tag="KB950_001577"
     CDS             complement(1425..1709)
                     /locus_tag="KB950_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857799.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1722..2285)
                     /locus_tag="KB950_001578"
     CDS             complement(1722..2285)
                     /locus_tag="KB950_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6857800.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2513..3175
                     /locus_tag="KB950_001579"
     CDS             2513..3175
                     /locus_tag="KB950_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBB6857801.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3299..4495
                     /locus_tag="KB950_001580"
     CDS             3299..4495
                     /locus_tag="KB950_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBB6857802.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4596..5972)
                     /locus_tag="KB950_001581"
     CDS             complement(4596..5972)
                     /locus_tag="KB950_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6857803.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6009..6876)
                     /locus_tag="KB950_001582"
                     /pseudo
     CDS             complement(6009..6876)
                     /locus_tag="KB950_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6965..7990)
                     /locus_tag="KB950_001583"
     CDS             complement(6965..7990)
                     /locus_tag="KB950_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6857804.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8008..8682)
                     /locus_tag="KB950_001584"
     CDS             complement(8008..8682)
                     /locus_tag="KB950_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6857805.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8866..9759)
                     /locus_tag="KB950_001585"
     CDS             complement(8866..9759)
                     /locus_tag="KB950_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6857806.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9833..11530)
                     /locus_tag="KB950_001586"
     CDS             complement(9833..11530)
                     /locus_tag="KB950_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6857807.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11523..12836)
                     /locus_tag="KB950_001587"
     CDS             complement(11523..12836)
                     /locus_tag="KB950_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBB6857808.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12836..16873)
                     /locus_tag="KB950_001588"
     CDS             complement(12836..16873)
                     /locus_tag="KB950_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB6857809.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16886..17344)
                     /locus_tag="KB950_001589"
     CDS             complement(16886..17344)
                     /locus_tag="KB950_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6857810.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4908 a         3904 c         3028 g         5612 t
ORIGIN      
        1 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
       61 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
      121 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
      181 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggaatc
      241 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
      301 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
      361 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
      421 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
      481 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
      541 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
      601 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
      661 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
      721 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
      781 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
      841 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
      901 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
      961 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     1021 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     1081 gggttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     1141 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     1201 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     1261 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     1321 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     1381 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     1441 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     1501 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     1561 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     1621 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     1681 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     1741 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     1801 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     1861 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     1921 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     1981 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     2041 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
     2101 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccgccagg atgaaatacc
     2161 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
     2221 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
     2281 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
     2341 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
     2401 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
     2461 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
     2521 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
     2581 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
     2641 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
     2701 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
     2761 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
     2821 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
     2881 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
     2941 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
     3001 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
     3061 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
     3121 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
     3181 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
     3241 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
     3301 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
     3361 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
     3421 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
     3481 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
     3541 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
     3601 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
     3661 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
     3721 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
     3781 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
     3841 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
     3901 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
     3961 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
     4021 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
     4081 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
     4141 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
     4201 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
     4261 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
     4321 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
     4381 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
     4441 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
     4501 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
     4561 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
     4621 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
     4681 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaaaatgga gacgattggc
     4741 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
     4801 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
     4861 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
     4921 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
     4981 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
     5041 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
     5101 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
     5161 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
     5221 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
     5281 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
     5341 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
     5401 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
     5461 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
     5521 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
     5581 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
     5641 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
     5701 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
     5761 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
     5821 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
     5881 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
     5941 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
     6001 aacagcgatt aaactgtttt catataccct aaaagggata ccacctaaaa tgactaaaag
     6061 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
     6121 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
     6181 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
     6241 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
     6301 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
     6361 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
     6421 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
     6481 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
     6541 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
     6601 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
     6661 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
     6721 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
     6781 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
     6841 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
     6901 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
     6961 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
     7021 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
     7081 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
     7141 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
     7201 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
     7261 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
     7321 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
     7381 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
     7441 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
     7501 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
     7561 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
     7621 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
     7681 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
     7741 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
     7801 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
     7861 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
     7921 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
     7981 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
     8041 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
     8101 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
     8161 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
     8221 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
     8281 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
     8341 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
     8401 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
     8461 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
     8521 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
     8581 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
     8641 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
     8701 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
     8761 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
     8821 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
     8881 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
     8941 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
     9001 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
     9061 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
     9121 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
     9181 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
     9241 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
     9301 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
     9361 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
     9421 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
     9481 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
     9541 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
     9601 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
     9661 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
     9721 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
     9781 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
     9841 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
     9901 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
     9961 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    10021 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    10081 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    10141 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    10201 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    10261 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    10321 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    10381 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    10441 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    10501 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    10561 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    10621 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    10681 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    10741 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    10801 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    10861 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    10921 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    10981 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    11041 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    11101 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    11161 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    11221 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    11281 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    11341 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    11401 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    11461 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    11521 ttttattcat ttttttctcc ccgttctttt tttattaata aaacagcacc taacaataaa
    11581 agaattccta gtaaagaaat caacgttctt gcttcattcg tttgcgggaa tacggaccat
    11641 tttcctgatt gacctggatt gcttggacca ctcggctctt tcggtttgtc cgttgtttct
    11701 tttacggtct cgttataaac acgtggtgtt gcggattcat aggcagagac tggaactttc
    11761 ccggattcta attcagccat ctcctgtccg ttaactacta catattgtgg ttggcctttt
    11821 gcatccaccc aaaattcagg gatgatgacc tcgattctct tacttgcttc atccatctcg
    11881 taaccttcca caccttttaa ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt
    11941 cctgtcgtaa caaggccctc tttatccgaa atgaatttgc ttacattttt gtcattcaaa
    12001 ggatcattgt ctgctggttt gatccattga tttttcaagt cattagcagg agccatatct
    12061 aaataatatt tccccccttc tatcatctga tatagagcaa aaacagcacc ttctaatggt
    12121 tttccttttt cagttgttcc tctttgacgt ccatacttga agaagtatgg atctcttaga
    12181 taaccgacat ttttaggata gatatgaatc tcttgcagtt cctcggttga ttctgtcggg
    12241 tgataaattg gcaaaatagc agcaattggg atagctgtct gatcgatatc aacatccaat
    12301 ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt
    12361 ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc cgccaaccgt atctttccca
    12421 gttgttaccg aagtccctat ttggacaagc ctttcttctg tgattagctg acggatattg
    12481 tttctgttcg tttcagcgat ttgctggacg aattctttag gagtcatgtt ttcagcagct
    12541 tttttttcat gataataatc ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc
    12601 accgtctgtg aaatgatatc tgctgtttcc cctgcttcct cagagatagc caagccatca
    12661 ttttcatggt attcaaattg atcgttataa tcgatatctc gaatcatccg tttatgcaac
    12721 acgaattcca cgacatcttc ccctgattct tctgccgaca tcgcatgtgg aagaaggact
    12781 gctgcaagcg gtagtaaaac aagtaattga agcatcaaac acaacagctt tttcattacc
    12841 ttgctccctc ctttttagat tgtctgacaa acaaaccata ggctgctcct gcaagcgcca
    12901 tgaagaatgc agcaagcagg atatagaata atcgtccgga tcctcctgta gcaggcaacg
    12961 gattttcagg atcgttcccg acactaagct gtaatctgat tcgatttgtc tccaaaagtt
    13021 catattctaa ctcttgctgt agatcaggcg tctctgcttc ttcgtttgga taatacgaaa
    13081 tctcaacttt tagtgtaccc gcttcttttc ctggaactac ccgatcattt tctatgtcag
    13141 gttcttctcg ataactaatt tctaaaataa agtaacctgg aagttttcta tagctgtcag
    13201 gtgacttcac ttccttgatc cgatagaccc cttccaacaa gtctgaagca ttgaataggg
    13261 aagaatcgtt tggatctggt gccaagcctc cctcgatgat ttcatactct cctgcactat
    13321 tttgtttctc aattgaaaat tctgcgcctt caagacgtcg gctattcata ctgtcaattt
    13381 tcaaaagttc caaatcgatt ggcgctagtt catcttctaa ggttacttgt agtacaccat
    13441 cgatgatttc atagccttcc ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa
    13501 tgataaactt accttcttca ttcagtgcaa actggaacac ttgatcagat agccgatacc
    13561 catttggtgc gacagattcc gagacctcat attcaccaat agacagatgc agaggttggt
    13621 cgtaatttaa atcgacatac ttttctactc ctacggcacg agaatagaag tatccgatcc
    13681 caggcaaacg tttcgattct tctggttcta acggattacc agtgtatgct cgatacagcg
    13741 ggaaggaaga atcccccact ttcctgatat cgaaacctgc accatctaat gtatggcctt
    13801 catggtctaa ttttctaact cggatctccc gataatcaaa cgtattgtaa gcagtgaatg
    13861 ctacccataa acgatcctct tcatctgttg tatacgtgag atattgccaa ttttcttcac
    13921 ctgtttgctt gagttggata gtccctatag cattatcgta aagtcgcaag atccatgtgt
    13981 cattttcatc aggttctttg aaaccttctg ggttctggct ctcaatgatt ttgtattctg
    14041 catagttggt agtttcttca ctatccttat aaacaaatga atctggctct ccgattggat
    14101 gaataacttt ctcaccatcc acgattacat atttcccgtc ttcatctact gaatagaacg
    14161 tgagtctccc tttctcttcc tctacttcgt tcacaccaga aagccctttc gctactggat
    14221 atccgatttc cagtccgtta ccatctgttt cataaagttc aaactctgcg ccattgattg
    14281 gcatattact gccttcaatt agtttattga tattcatgtg gattggcttg aattcatttc
    14341 gcatttccag tttgacatac aactcatcat actctgtgcc ttctatgtta ggctcttgta
    14401 caacagaaag cttgtgatat tctccttcat aataggaatt cggatcttta ccttccggtc
    14461 cgccgactct cgaaccagat gtatattcat ctacaagctc gtagtgacct aacatctcat
    14521 cttcgttggc tgcatatttt ttgatttgga attgatacgt ctcatctact aactgataac
    14581 gtggtggtcc agcgatctct ctgatccaaa atatgtcgct gactgctcca gtttcatctc
    14641 ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt
    14701 cgattggttc ggcttcccca ctctctggat tttggtcata taatgcaaat tcgactccag
    14761 ccaacggctc gccattttga cccttcttgc tgacttggaa attacggcct ttatgttcgt
    14821 aacgaatgga agaagatggg aaatacatcc gtccagtagt gatatcattc tcataatcct
    14881 ccaggttctg aatataggcc atattgttca ctgtgcaaaa atttccgtct tgagctcccg
    14941 catcaagttt tacttggtac ttgatcctga ccatttcccc ttcacctaaa gaaatcggag
    15001 aaaatttata agcttggttt tcccgtccga catacgttgt cggagtcgcc actacttctg
    15061 gtggggcttg tccatgacgt tcttcatccc atatttctgg ctcttggtac ttcatgccgt
    15121 tttctcctgg atctagatgg taaaactgaa tatctgccga tctgccagct ccaaatggtg
    15181 agacattaaa accagtttca tcataaagtg tggcttgctg gatcgttctc ttgaaggtag
    15241 aagagatatc ctccaaggct gtaacaatgt cattcttgtt gttcgcattg taatatcgcg
    15301 gtgttacgcc atcttcttct aaatcagcaa tatttttcaa tacatttcgt ccaatagcat
    15361 ctagtcgttg tctcccgcta acgtttccat cgattcctaa accaatggag aagatacttg
    15421 gcgggttctc ttgatacttg tttttcagcc aatgatgata agcaactgtg ttgatcgcag
    15481 atgacttttc ttgcgaagta ggactgccat tcgtattgtt attagaagaa ccatcaccaa
    15541 cccagtgagt cgctttatta tctccttctt ttacctctcc ataacgatag tgtacattta
    15601 cttcatcttc tggacggtta aagtcagatg cataggtaac tggatgagta aaccctgcat
    15661 tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa
    15721 tcttatcttc catcacagtc cagttgctcc aagctccatt agtgctcgta cgataacttg
    15781 actgaatcgg tgcataactg aaggtcggtg tcccatctcc cacaacgatt aggatttttt
    15841 ctggatttct attttctccg ccattgtctt tatacaatgt ttcatagcca tttttaagcc
    15901 ctagtgtaag cggagttcct ccgattggtg tacgatttaa tgctgtattc tcacttcccg
    15961 tcaaacgatt gatatcattt gtcaatggaa agatgtccga actgatttgc tcatggttat
    16021 tgatagctgt actattatgg taaaaattca ccataccgat tcgaatatcc agactcgggt
    16081 cagagagcag atctttactc atttcgttta ctgcctcaat caacgcagca tttttagttt
    16141 gactattggt cccagcggtt aattcactca tactcgctga cttatctaga acaaaaacga
    16201 tatcgattgg actgatttcc tttttctcgc ccccgatgac atctagatag acattgtaaa
    16261 gggttgttgc atcttcagga tgtagatcag atgctgtgct gcttggcaga acaggttcta
    16321 cccatttttt gatgtacgcc ccgttataat ccaagtaact gttttcaaaa ttccgttcat
    16381 ttccccaaag ctgaacggtt tgagctggaa cgtttctatc ttcttcgcct gcagttgatc
    16441 cattattagt agcatagtcg taatttcgcc agttcgctcc cccgtttgta taatcactat
    16501 catcgatcaa gttctctccg tcatatggcg agcctttgtc tccttgatag cgttcattgc
    16561 tttgtgtcca atttcctaac acttttgtat agtcattcgt tgtaaacttc gggtagcgat
    16621 gctgttcgtt ttcaacttct ccgctaggat agatatattc ataggggtct tgcgtagctg
    16681 ttggcccaag acgctccaaa ataccgttga ataactggcg ttgaatgcgc tggagtgctt
    16741 gaccgctggg tacgattggt tcgtccagct tttccatctc attttcttcg ctacgttgaa
    16801 tttcagcgga atcttctgta tcagtttcat tttcttgtaa ttcttggtta tctattcttt
    16861 catcaaccac cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt
    16921 caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt
    16981 gaccatcgat cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg
    17041 ttccacgaac gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta
    17101 ttctaccttc attggcactt tccttttttt caaaagccag atgccattca agggtttctt
    17161 ctttttcaat atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct
    17221 cctttgtttc tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga
    17281 aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt
    17341 tcacatcctt atactccttt caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa
    17401 tgcccatcta attataattt aatttaattt taattttaaa tcaaatgata tc
//
LOCUS       DADVXO010000058        17092 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.060, whole genome shotgun sequence.
ACCESSION   DADVXO010000058 DADVXO010000000
VERSION     DADVXO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.060"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(150..2234)
                     /gene="cadA"
                     /locus_tag="KB950_001590"
     CDS             complement(150..2234)
                     /gene="cadA"
                     /locus_tag="KB950_001590"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBB6857811.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2759..3118)
                     /locus_tag="KB950_001591"
     CDS             complement(2759..3118)
                     /locus_tag="KB950_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBB6857812.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3148..3489)
                     /locus_tag="KB950_001592"
     CDS             complement(3148..3489)
                     /locus_tag="KB950_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857813.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3486..4160)
                     /locus_tag="KB950_001593"
     CDS             complement(3486..4160)
                     /locus_tag="KB950_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6857814.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5336..6634)
                     /gene="asnS"
                     /locus_tag="KB950_001594"
     CDS             complement(5336..6634)
                     /gene="asnS"
                     /locus_tag="KB950_001594"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBB6857815.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6674..7864)
                     /locus_tag="KB950_001595"
     CDS             complement(6674..7864)
                     /locus_tag="KB950_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6857816.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7885..8412)
                     /locus_tag="KB950_001596"
     CDS             complement(7885..8412)
                     /locus_tag="KB950_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBB6857817.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8459..11248)
                     /locus_tag="KB950_001597"
     CDS             complement(8459..11248)
                     /locus_tag="KB950_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBB6857818.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11395..11595)
                     /locus_tag="KB950_001598"
     CDS             complement(11395..11595)
                     /locus_tag="KB950_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6857819.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(12048..12368)
                     /locus_tag="KB950_001599"
     CDS             complement(12048..12368)
                     /locus_tag="KB950_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857820.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12871..13989)
                     /locus_tag="KB950_001600"
     CDS             complement(12871..13989)
                     /locus_tag="KB950_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857821.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14460..15767
                     /locus_tag="KB950_001601"
     CDS             14460..15767
                     /locus_tag="KB950_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBB6857822.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15888..17087
                     /locus_tag="KB950_001602"
     CDS             15888..17087
                     /locus_tag="KB950_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBB6857823.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
BASE COUNT         4842 a         3621 c         2988 g         5641 t
ORIGIN      
        1 aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt ttgaattttc
       61 tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac atttttcatt
      121 aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac atgcaagctt
      181 cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac tgcatctccc
      241 attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc aatagcaaag
      301 acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc ttctgcaatc
      361 ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc atcgcttccc
      421 aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc gttgattcca
      481 tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa ccgctgcact
      541 ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt cccgatatga
      601 tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc agcctgtttt
      661 aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac aagataacca
      721 ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact taaaggaaaa
      781 gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt gccatcaact
      841 tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa aagcggacct
      901 gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca
      961 atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat cgaaaagtca
     1021 cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc aagcaactcc
     1081 aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat accgccaaag
     1141 aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa aaatgtcaat
     1201 gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg taaaacagct
     1261 aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa
     1321 ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa tttgctgata
     1381 gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt attgataaat
     1441 ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc tgtaagtgct
     1501 gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac acgttcacca
     1561 ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt catcaatcct
     1621 tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg aatcgatttt
     1681 cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa tagcatcact
     1741 gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac agacataaga
     1801 aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt cactacatca
     1861 tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc attaaaatca
     1921 aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag cgtatttctc
     1981 tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac tttgacatct
     2041 ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc
     2101 tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt gactccattg
     2161 atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa accttctaat
     2221 ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc ttcataacta
     2281 attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct
     2341 gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt ctcttcgatc
     2401 attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg atagcaacta
     2461 agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat aggacaatat
     2521 cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact acgctttcaa
     2581 aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca gtaatcccat
     2641 ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac atagagtcag
     2701 gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta ttctcttttt
     2761 atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg
     2821 caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag
     2881 caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact
     2941 ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt
     3001 gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc
     3061 taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg
     3121 tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct
     3181 ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc
     3241 ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca cccgttcttc
     3301 tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac
     3361 gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc
     3421 tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccgtat
     3481 ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg
     3541 gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc
     3601 ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatgcca
     3661 tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta
     3721 gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc
     3781 atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg
     3841 gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc
     3901 caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct
     3961 tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat
     4021 tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct
     4081 ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct gtcaccgata
     4141 aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt
     4201 ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga
     4261 caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta gttttttata
     4321 ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc cagagaaatc
     4381 gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac atatatttat
     4441 aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta gattgcttgg
     4501 gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta aacttacata
     4561 tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac tattagtttg
     4621 ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa tcagatttcc
     4681 atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata gaaccttgtg
     4741 aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc ctttatcccg
     4801 tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa cgttgctctc
     4861 cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa
     4921 attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata taaacggtgt
     4981 tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa gctatttttc
     5041 cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt ccttaaacgg
     5101 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt
     5161 tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct ctccactaaa
     5221 cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa catgaacagc
     5281 tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc acctcttatg
     5341 gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg tcgataccag
     5401 caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga acagaaccgt
     5461 atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga ttacggattt
     5521 gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt tcaccgtaac
     5581 cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga tgtggtttca
     5641 tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt tcgaatgaat
     5701 tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg tcatcaaatc
     5761 catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat ggcaattttg
     5821 tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat tcgcaatgat
     5881 ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa ctttcttctt
     5941 gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga cgtcgtgttt
     6001 ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa gccatagcag
     6061 cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca aagtaatttg
     6121 tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc ggatcgattt
     6181 tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca tttctgatct
     6241 gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc atcaagaaat
     6301 ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc acttccacat
     6361 ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt tctccagtta
     6421 ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct tccgacactt
     6481 cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt aaaaatgcga
     6541 tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt tctcccacat
     6601 ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt atattccttg
     6661 aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt ctgcgtaccg
     6721 cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct ggtgccccaa
     6781 agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct acccattgtg
     6841 tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa agatagaaag
     6901 ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga tataacgtat
     6961 tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca cctttcaatg
     7021 cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg gaagcaacag
     7081 cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc cagcctgtca
     7141 tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt gcttctgaaa
     7201 tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa atatcatcag
     7261 caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt tcattcttgc
     7321 tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt gttttttctg
     7381 aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct tcccctttca
     7441 caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc cagtaaggca
     7501 caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta tacaaagcaa
     7561 atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca tagtttttct
     7621 tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt gtataatagc
     7681 tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct ggtgtcgcaa
     7741 aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt aatgccttgg
     7801 cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt tttgataact
     7861 ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt catctccaga
     7921 ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta ctacttccca
     7981 cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt caggatgggc
     8041 cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat ccaatacttt
     8101 tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat catttgtacc
     8161 tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg tttcctcaag
     8221 atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg ccggacgact
     8281 ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata cgatcaagcc
     8341 aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg gtcgtcgctc
     8401 ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga cagctcctct
     8461 aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt tcctccatag
     8521 ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg ccatattttg
     8581 cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc gtccttatca
     8641 agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc tggtaaaaag
     8701 gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg cgaagcggat
     8761 tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc aaatctacac
     8821 cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg aagcgtttca
     8881 aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct ttttctagca
     8941 tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg aataacacaa
     9001 gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa taatcgatat
     9061 atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag atccttgcct
     9121 gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca atgccaagcc
     9181 tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata taaagaatac
     9241 gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa tcatggagat
     9301 gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa tgatctcgcc
     9361 agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca aataggctat
     9421 ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata atctcaaaag
     9481 tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc aggtgatcaa
     9541 tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc atgacttctt
     9601 ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct tctgccatac
     9661 aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac cagcgtaagg
     9721 cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt ggttcttcta
     9781 attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt actttttcag
     9841 cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct ggaagcaatg
     9901 gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa aaattacttt
     9961 gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac tgatttcttg
    10021 ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc aaatttgtta
    10081 aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag accgccattt
    10141 gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta aaacgctgca
    10201 aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga tctagcaatt
    10261 gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc gaaacggtac
    10321 tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga taaccgattg
    10381 ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct tttgtgaaat
    10441 ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt gttttaccaa
    10501 acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa tggacggatg
    10561 tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct aatgagggat
    10621 caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa cgactcgtat
    10681 ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt ggcaattttc
    10741 tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca ctatctgcct
    10801 gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag cgaaagcttg
    10861 gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt atcttcaaac
    10921 taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa taaatattat
    10981 gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca tcttcgaaat
    11041 aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt tgttttgaaa
    11101 tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct gattcaatca
    11161 tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc gtttcgatat
    11221 caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc tctaatgttc
    11281 ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa agaaaataga
    11341 caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg ttaattaagc
    11401 tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga aagttactgc
    11461 ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat gaacgaatac
    11521 atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa accattttac
    11581 tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt gcaattactt
    11641 gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt accgaacaaa
    11701 aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc gacttttttt
    11761 attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt ttttaaaata
    11821 aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa actaaaaaaa
    11881 ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg aagattcttt
    11941 agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca gaagaacaag
    12001 gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta tacctcataa
    12061 ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc tgcagctgta
    12121 gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca aaagtaacca
    12181 aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag atctggtgtc
    12241 tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg tgtttcttca
    12301 gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca taacagtctt
    12361 tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata tctcaagaac
    12421 gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg tcaaaaagag
    12481 gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct tcttcggaaa
    12541 taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc ttatttcttg
    12601 aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    12661 gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt tcttattact
    12721 tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc tgaccttcgg
    12781 tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt ctctaatact
    12841 caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg tcagactctt
    12901 ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa tgatcacatc
    12961 atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat cttcaccgaa
    13021 accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt ctttcgcagc
    13081 tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat tgtattcttt
    13141 tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga tatctctaaa
    13201 ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg gatttttagc
    13261 aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg gccattgttc
    13321 tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt tgacccaatc
    13381 gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat ctcgggctcc
    13441 gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc ccaatgcagt
    13501 aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt tgttgctgac
    13561 ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat agttgaactg
    13621 cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt gatcgtctac
    13681 cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg ctacactcgt
    13741 agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt cttggaacgg
    13801 ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt tcgcaaaatt
    13861 tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat tcagcatgat
    13921 cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt ctttatcctt
    13981 ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc taaagtctac
    14041 cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg tttctacggt
    14101 tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt tagccccagt
    14161 cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag aggtggatct
    14221 tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg ctttcagcgc
    14281 ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta aaaacgaccg
    14341 ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt gaaactctct
    14401 cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg agatcggata
    14461 tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt tgccttgctt
    14521 cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat gcaaaagcag
    14581 cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga gaaaaaacca
    14641 tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat caagtaaaag
    14701 aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa aatttgagtg
    14761 tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc gttaaagaat
    14821 tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg gcagaaaaaa
    14881 tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa gattggggta
    14941 tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt ggcgaaaacc
    15001 gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat gtggctatcg
    15061 ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc actacgacac
    15121 aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac tggatgctcc
    15181 caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg actgaacttg
    15241 caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc actgtcgtat
    15301 tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca gctcgcttaa
    15361 tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat gcaagtatcc
    15421 ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc gttgtgctta
    15481 aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta acaatcacac
    15541 caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg gataaaggaa
    15601 caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat cttgaagaca
    15661 acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca aatattttcg
    15721 ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg aacaaaaaga
    15781 aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa ataacagctt
    15841 ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg aatctaacca
    15901 gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt tattggcgaa
    15961 aacagaaaaa ggatccttcc ttgttctttt acagcagtac atggacgttt gcggtaatct
    16021 tgacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg attcccttag
    16081 tgtttttcag tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg ttgaatggtc
    16141 ttttattcaa tattttcctc attagttttg gtatctactt gtttgtcttg ctatacgaaa
    16201 cacaaaatat ttttcttggc ggactgattg ccttgatcac gatcccgtta ctgcttgtct
    16261 ttctattcgg catttatgga ttgatcgttt ttcttttttg gaacggtgtc acagttttac
    16321 gtagagagtc gcattcatta gctaatcttt taacgttgat actagccatt tttcttacat
    16381 tgtttcttgt ttttgacttt ttcttgttaa aatatttacc acaatggata aatgctttgt
    16441 ttttctgcat cccattgatc ttgatctatc tatttatcgt attctacaac tttttgactg
    16501 tctcattctt gtatcagttc aaccgccctc gatacaacca agacttcatc gttgtgttag
    16561 gcgccgggtt gatcaatgga gaaacagttt ctcctcttct tgccaagcga atcaataagg
    16621 cgattgcttt ttattgggca caaagccgtg cgacattaaa tccgcctatc ctattaatgt
    16681 cgggtggaca gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa caatatgcca
    16741 tggaacaagg aattcctgaa agagatattc ttgtcgaaac caattcgaca accactttgg
    16801 agaatatgcg ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc tatcgcgcga
    16861 tcttcagttc caacaattac cacattttca gagctggtct ctatgcacga caagcgaaac
    16921 tgaaggcaaa tggcattggg gcaaaaacag ctttctatta cttgcctaac gcgtttttaa
    16981 gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt tgcggattgc
    17041 ttttcgcagg ttccgcattc ttatcactga tcactttact tttctaaaaa aa
//
LOCUS       DADVXO010000059        16843 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.061, whole genome shotgun sequence.
ACCESSION   DADVXO010000059 DADVXO010000000
VERSION     DADVXO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.061"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..134
                     /locus_tag="KB950_001603"
     CDS             <1..134
                     /locus_tag="KB950_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6857824.1"
                     /translation="NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            277..420
                     /locus_tag="KB950_001604"
     CDS             277..420
                     /locus_tag="KB950_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBB6857825.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            998..2581
                     /locus_tag="KB950_001605"
     CDS             998..2581
                     /locus_tag="KB950_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6857826.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3018..4202)
                     /locus_tag="KB950_001606"
     CDS             complement(3018..4202)
                     /locus_tag="KB950_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBB6857827.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4227..5234)
                     /locus_tag="KB950_001607"
     CDS             complement(4227..5234)
                     /locus_tag="KB950_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6857828.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(5406..5765)
                     /locus_tag="KB950_001608"
     CDS             complement(5406..5765)
                     /locus_tag="KB950_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857829.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(5737..6108)
                     /locus_tag="KB950_001609"
     CDS             complement(5737..6108)
                     /locus_tag="KB950_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBB6857830.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(6125..6427)
                     /locus_tag="KB950_001610"
     CDS             complement(6125..6427)
                     /locus_tag="KB950_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857831.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(6444..6914)
                     /locus_tag="KB950_001611"
     CDS             complement(6444..6914)
                     /locus_tag="KB950_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBB6857832.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(6889..7167)
                     /locus_tag="KB950_001612"
     CDS             complement(6889..7167)
                     /locus_tag="KB950_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBB6857833.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(7164..8213)
                     /locus_tag="KB950_001613"
     CDS             complement(7164..8213)
                     /locus_tag="KB950_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBB6857834.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(8170..9099)
                     /gene="tadA"
                     /locus_tag="KB950_001614"
     CDS             complement(8170..9099)
                     /gene="tadA"
                     /locus_tag="KB950_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBB6857835.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            9231..10985
                     /gene="ade"
                     /locus_tag="KB950_001615"
     CDS             9231..10985
                     /gene="ade"
                     /locus_tag="KB950_001615"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBB6857836.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(11083..11745)
                     /gene="trmB"
                     /locus_tag="KB950_001616"
     CDS             complement(11083..11745)
                     /gene="trmB"
                     /locus_tag="KB950_001616"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBB6857837.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11815..12597)
                     /locus_tag="KB950_001617"
     CDS             complement(11815..12597)
                     /locus_tag="KB950_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBB6857838.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(12801..14012)
                     /locus_tag="KB950_001618"
     CDS             complement(12801..14012)
                     /locus_tag="KB950_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6857839.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(14005..14742)
                     /locus_tag="KB950_001619"
     CDS             complement(14005..14742)
                     /locus_tag="KB950_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6857840.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            14880..15314
                     /locus_tag="KB950_001620"
     CDS             14880..15314
                     /locus_tag="KB950_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBB6857841.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            15307..15651
                     /locus_tag="KB950_001621"
     CDS             15307..15651
                     /locus_tag="KB950_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBB6857842.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            15831..16841
                     /locus_tag="KB950_001622"
     CDS             15831..16841
                     /locus_tag="KB950_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6857843.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         5379 a         3447 c         2960 g         5057 t
ORIGIN      
        1 tcaaccaagt caatcaagga ttcatctcat ccgttgcgtc caaaagaaac catatcccta
       61 gaaaatcact aaattaccaa acaccattag aagttttttt gagttacgta aatggaaagt
      121 tttgtctcgc ttaatttgac aaataatatg tttaaaataa agggaaaaca gtcaatgagt
      181 tcaagtaaaa taaaaaaaag tgaacaccag ggcgattagt cgccctggtg tggtttaaaa
      241 cgacctaagc atgccaaagc ttattggttc gtcaatgtgg aagtttccga tgaatctgat
      301 acgaaaaaat atgaagtatc gatggcggat accgatttta tcgccaaact aaaattttat
      361 ttcgggttcg ggtaccgcga gaaggcaagt gctacattcg gtaaatcatt caagaattga
      421 tgaaaggcta ccgcttgcca ttgcaaccac aacgatactc ctgaacgcga tgttggtgag
      481 atttccaaag agaagagctg tttaatgcaa cggtattaag caaatggcaa aaggaagtca
      541 tgtagacaaa gttttttatc aaacaccata tgatttcgcc tgaacgctgg tttagctcag
      601 aatacaatga tgtggtccac gagacattac cctcagtcag ataaaaaaag ctctctgaca
      661 gaattacaca tcgataaata ttaagtaaat gtcagcgcta gttgaacttt ctgacacttt
      721 caggtacact ggaaaagaca actacacgaa ggagggaaaa tactatgatg cgcatgacta
      781 aaataacaag taatgaaaat taccaacgat accatatcgc atttagccat gcaattggtt
      841 ttacagttgc tgttgacggg acaagtttgt ccaaaacagt tgaattcacg taaaatcaat
      901 ggccatctca aggtattttt ctcatgataa tctgagttta aataacacag gccatggata
      961 accatggcct ttttgagtgg tctcaaggag agagatcatg atcatacaag caatttctat
     1021 taaaaaaaca atcggcggga atccattatt tgaggattta tcctgtcaaa ttgaagctgg
     1081 tgaaaaaatt gtcttagtcg gtatcaacgg aactggcaaa tcaacctttt tacaaatctt
     1141 gaccggcaga gaaggtgtcg acagcggtgt tatcagtcgc aaaaaaggct taaagatcgg
     1201 gacggtcgaa caagaactta cagtttccga agcgaccgtc aaccactact tgctccattc
     1261 tgccgctgag atacaggacc tcaaacagca aatgagtcgc tacgaagcat tgatgacaga
     1321 accagataca aacttagaga agtgtctggc ccgttacggt gagctgcagc atcgctttga
     1381 agaactaggc ggttacgtat tagaagaccg gattgcagcg atcttgcaag gcttagggat
     1441 tccacaccgt caggaagcac ggttaacaga acttagcgga ggcgaacgag ttaaagtcgc
     1501 tttagccaaa attttagcag ccgatgccga tttactttta ctcgatgaac cgaccaatca
     1561 tttagatctt aacagtattc gctggttgga aaactattta cagaatacaa aaaaatcggt
     1621 tctggtcgtt tctcatgatc ggcaatttct cgatcaagtc accacaaaaa catgggaact
     1681 ggaagaaggt gcattgattg agtatcccgg aaactacagc cgttttcgag tcttgaaaga
     1741 agctcgcttg gctgagctga caaaaaatta cgaactgcag caaaaagaag tccagcggtt
     1801 gaaagtcatg attcgtcgtt ttcgtcagtg ggcacatgaa ggagacaatg agtccttctt
     1861 caaaaaagcc aaagaactgg aacgccgcct ggcgaaactg acactcgtca aaccgccacc
     1921 tcccccgaaa aatcggctgc aaagcttgtc taatggtgga aaatctggca aagaagtttt
     1981 catcattcaa aacttgcacc agcagtacgc tgatcaagta ctattcaaag acagcagttt
     2041 tgcagtctat cgtggagatc atttagccat catcggcgac aatggtgcag gaaaatcaac
     2101 cttactcaaa ttattactcg gtgaggaaaa gccttacggt ggcaccatca aattaggtag
     2161 tagcttgcaa atcggttatt tgccgcagca gctgcagttt gccgatcccg atagccgttt
     2221 gctggcttac gccatcacat tgactggaaa cgaagaagtc gctcgacggc aattagccaa
     2281 aagtggcttt tatcaaagcg atgtgggtaa acggatcaaa gatttatccg gtggtgaaaa
     2341 aatccgtttg gcgttgctga aactcttttt agaaaaaatc aacgtcctga ttttggatga
     2401 accaaccaat cacctcgaca gctatgctcg agaagaaatt gaagaaatgc tgcaagacta
     2461 tcaaggaacg ctcctagcag tcagccatga ccgctacttt ttgcagcaac acttccaaga
     2521 agcactcgtt attgatcagg agcagatcag ccgccagccg ctcggccaac tgattgacta
     2581 ggacgttttt tcgcaacaaa aaacttgatt ttcatacctg tgctttcacg attcaggcaa
     2641 agtacaggtg gaaaatcaag tttttattat tcgatcaatg aatgtttcgg atcggttttc
     2701 tagctaaaca taaagcgaag aataaagtcg acagcccacg aggctgatct aaaaatgttt
     2761 tttcttttta catatgtaat gtcccttata aagatgaatg aaaataaaat attgggattt
     2821 tcctgaaaat caataaataa aatgccagtc ttttcccttt tgaccacttt tccaagcaaa
     2881 attccctaag aagatcctgt aaaaaagaag ggaaattgaa gttaaattac cattttgcgg
     2941 tcttctccgc aaaaaaaaca catgcaagaa ttccctcttg catgcgtttc atcttttatc
     3001 ctaacgaatg ctttggctta gccgcgcaga cgctccacat cacgggcaat cattagttcc
     3061 tcatctgttg ggatcactaa tactcttact tttgattctt ttgtagagat atctacttct
     3121 tcaccacgca attcattcaa cgctggatcg atttcacaac caaaccaaga catacctttg
     3181 atgatttcac tacgtacatg tccgtcattc tcgccgatac cagcagtaaa gacaattgca
     3241 tcaacaccat tcatcgttgt cacataaccg ccgatgtatt ttcggatgcg gtccacgaaa
     3301 atgtcataag ctaaacgaat atgttctttt tcatagttct tttcaagatc acgcatatca
     3361 ctagacaatc ctgttaggcc tagcagacca gatttcttgt tcaaaacatt gatcatttca
     3421 tcaatatcaa tctctaattt ttccattaaa tatgggagaa cagctggatc gatatcacct
     3481 gaacgtgtac ccattgtcac cccagctaat ggtgtgaatc ccatagaagt atccatggat
     3541 ttacctccat cgactgctgt gatcgataca ccatttccta agtggcaagt gatgattttt
     3601 agttcttcga tcggtttgtt caataatttt gccgcacgtt ccgacacata acgatgactt
     3661 gtgccatgga agccatattt tcttactttg aaatcttcat aataagacac cggtaagcta
     3721 tataaataat tttgctttgg cattgttgag tggaaagatg tatcgaatac agccacactc
     3781 aaaatgtcag gtaaaatctt tttaaacgct ttgatcccca ttaagtttgc aggattatgc
     3841 aaaggagcaa attctgctaa ttcagcaatg cggtccatta ctttctcatc gatcactact
     3901 gaatccccat aagtttctcc tccgtgaaca acacggtgcc ctactccggt gatttcatca
     3961 taagagctta aaatttgcag ttcaatcaac tgatctaaca gcatttttac tgctacttcg
     4021 tggttttcga tatccatgat ttgttcgaat ttttgattat cgccgtactt gatggtaaaa
     4081 atagaatctt gtaaaccgat acgttcgacg atccctttcg caactacggt ttcttctggc
     4141 atttgatata attgccattt caaacttgaa ctcccagcat tgattgcaat tgttttagac
     4201 atatgactct ccttctcgtt gaatgattac agagcagatg atttccagtt tttaaactcg
     4261 ttgaaaaatt ccgtcacctt ctctggttct tttaatgaag caagtttcac aagcaagact
     4321 tcttttactt gttgtgcttg tggtcctttt ttctgtaaga tcaaaatact tttttgcgat
     4381 tgtttattac ggaacaattc atctggcaat tgaatcattc cttgaagata accttcttct
     4441 ttaaaccatt ttttgatttc ttcactttga tccgtttcta aaaatccact cggcatcaaa
     4501 aacaaaccaa aaccatccgg cttcacatat ttcattgatt gctctagcaa taaatgatgt
     4561 gcataactat ggccttcttc ggtacttgtt aaaaactctt ttgctttttc gtcattcgga
     4621 taataaccga ttggcaaatc gctgattgca aaatcgacag gatctattaa taattcctgc
     4681 aacccgtctt gatgaaagta ttgaacattt gcttgtgtca aatcacttgt agaagctgcg
     4741 acagccaaca atgtatcatc aatatccaca ccaattcctt ctgtttggta ccccgcattc
     4801 gataggttta aaagaactgt taataacaag ttgcccattc ctgccgcaat atccagaatc
     4861 ttaaccggtg cttttttatt tgtgtatagt tgttcaataa gaaaaacaaa taaaaaaccg
     4921 atactatcag gagtgagttg atggttcgcc tgcaaatgtt ctgtctggct gcccttcaat
     4981 aaaagtaatt gagacagtct gcgccattct tctggctcga aagaaagttt cttcaactct
     5041 tcataaagag ctgtgatccg ttgcgttgtc tcttttgtcg gcactccatc caccacacgt
     5101 acttgataat catctattaa attttctgca ttttcgacat aggcttctaa aaaggaagtt
     5161 cccaatgcat tctgtaaaag ctgaatagct tccaggtttt gattaaaccc ttgttccatt
     5221 ttttctggta acatggtgca cctcatttct agatatatta taacaatttt ttcacctgtt
     5281 taattctatc gaacaattga caaaatttca atcgttttca ctgtttttta tttgtgaaac
     5341 ggcaaacatt tttttattaa aaacagtgtt ataacagaaa agagggcgtt ttcacgaaca
     5401 ttccatcatt ctgtactagt atctgtatca ttttttactt cttctgtgaa ccgaaagata
     5461 aacggtgaga cttccacctc taagaatagg gactgttgta cccactgata tgttacagat
     5521 ccagttgtgt agtaaaaaac tccttccgaa ctttcggcaa gcgtagactg ttccgataaa
     5581 gccatctgct tcattatttt tgctatataa taatttttga ttcgtatatt aagatccatt
     5641 gacaaacggt agctttctaa tgctgccatc aataaaaacg tacataaaag aaataaaagc
     5701 aagacagaaa ataaaataga gcccttataa cattttctac tgatcgtttt ttgtccatat
     5761 ggcatatcca tcttctccat tttcaaagtg tacataaaaa gaaattgtgt gttctttttc
     5821 caataattga agctcagtga tacttgttaa cataggttga tgtccgccgg aagttcttct
     5881 tttacgtatc attgcctgat atctttcgat gatatactgt ttatcctcga tcgtgtagtg
     5941 cagttgctga tttttcacag agacattgtg actgctcttg agttcttctt ctaattgtgc
     6001 taaaaatatt agccactctt tttgatcttt acgctgcaaa aattcactta cttggatggt
     6061 ttgctgaatc aaaagattga atgtgagcaa tagactgctc aatatcatca gtccaattag
     6121 acattcaatc agcgtaaatg ctggaattac ttgttttttc aatactgatc gcttctttct
     6181 ccatattgac aaacactttt tcgattactc cattattatc gacaatttgg atatcgtatg
     6241 ggatcatttc ttcccttcgc tctggtcttt tgtgatacat ccgctgcgtc ttgatatctt
     6301 cataaatcat tcgaataaat agcagttcct ccccactttt cttttctgcc tgaagcaaaa
     6361 acagctggcc aaagctcact aagtagacac agacagaagc gatcataagc gctgtaaggg
     6421 actccaacaa aacaaatcct gttttattca attctttttg tatagcgacc actccctatc
     6481 tggaattgat acgtaatcgt ccgctctttt tcttcccaat aaaattgata agcttttagt
     6541 gaactttcat tcccggttct agcagcaaaa gtaatagaag catggcgttt caaggtaatc
     6601 tcatcaggaa tatctaatac ttcccaattt attttatctg gattcggaac atgaaaaatg
     6661 atttgattct cttcactatt ccaataaaag cctgtttgga tctgattcac aatagcgatt
     6721 ttttgcgtag aatagattcg tttttcaaat tggccgaaaa attgatagac tgccaattca
     6781 ttcttccatg cagaaaatgc gagaactgga aaagaaagga tcgttgtaag taataaaatc
     6841 agtaagagtg attcaaacaa cgtatatcct gatgctaaaa ccttaccttc aaggccgctg
     6901 atagatttct gcatgttctc gtttgatata gccttcattc aacaattcct ccacagtagg
     6961 aactcttcca ttattttgaa gcgcatagag ctctttttgt gtgtctacca actggatgat
     7021 cgcttcacga ctttcttggt ctacatgatt cctataacgc gacaaattag ggacgaacaa
     7081 caacactaat acactgatga tcagtaagac gattagcatt tcgatcaatg taaaaccgct
     7141 ataataagat atttttctct ttttcatggc aaaacctcct ctatgcttcc gtaaacaggt
     7201 aataaaacag cggcataaac taataaaatc aaagccgcta cgcccaaaaa aatgattggc
     7261 tgaataaaat gcatccagtg atgaagacgt ttgaaaagct gttttcttgt ccattcgctg
     7321 taaaaaatca gttctttacc taaacttccc tttgcctctc cctgtaaaac aattttactg
     7381 aattcttctg atagaaaacg ataattctgg aactgctcag ccaatgacat acctgcctcc
     7441 aatccttgaa tcaacttaga ggcaagatgc tgaatcagac tttcttgttt cgtttccttt
     7501 aagcagacta tgatttgcct taattcgaaa ccttcatgaa aaagctttcc aatctctagt
     7561 gacaaataag ccgattgata caaggaatat atcggtccaa tcaataattg tctgcttatc
     7621 cattcactct gatcaacagg agacaacttc atcagacgat accttactag taaagccaat
     7681 agagtaaccg ctaggatgaa tagtagaaca taccaatgaa agacttgtat cagctgaata
     7741 ccccaatgct cttgcatgac cattcctgaa ccaataagct gaggcaaaac gaacaaccgc
     7801 atactaaaca agatacctag caaaaaggtg agcaatagca tcggatatgc aagcagcttt
     7861 ttcaaatcat tgcgaaatcc ttcaacaagc cgaaactgtt tggcaatttc caacaaagtt
     7921 gtcggcagat tcccatgacg ttcagctaac tcgatctgta ccagttgctc tgctccatat
     7981 cctatttgcg aaagtacctc actaaacgct tgcccttcct ttagctgatg ttggatattt
     8041 gccaaaataa caggtggaaa cgaacggatc gtgagtaata aataaaggga ttcttgcaga
     8101 ctaaaaccag ataaaagcag ttctcccatg gtttgtgcaa actggttttg ctggcttctt
     8161 gttaatcctt tagaatagtt ctgactgcca aatattttcc tgagcttctt cataactttc
     8221 ctcccatgat ttttttactg attcttcctc cataaattga taactccata aaacagaagg
     8281 atggtgattg gcatcttcca aaagttcttg ataaatgaca ccagctaaac attcagatag
     8341 ctcttctttt ttctttgtca gttcatggat tctgaccaat gtaccttcaa gattgcgtgc
     8401 atgtacggtt gcaaaaaccc ggtgccctgt tagtgctgct cttaccgcag catgtgcagt
     8461 tgattcatct cttatctcgc caatgattag caagtctgga cgatgccgta atgcgagtct
     8521 caaaagtgta tcataagtct gtgctatttt cggattgatc tgtagctgca gaaaaaacgg
     8581 ttcttctatc tctacaggat cttcgatagt aatcacttgc aatttttctt ccaaagccaa
     8641 ccgatacatt agagaagtct tgcctgatcc gactggtccg ctgaaaaggt ataatccacg
     8701 tctgacggtt ttctgtaaaa tagttgcaaa atctcgagaa ctaaagcaac gatggggtgt
     8761 ttcaaaagta ccgtataata gccgaatcac taaactctct cgttgaaggt aatctcctac
     8821 agaagacaac cgcagacgaa cttcctgttt ttctaaagaa taagtaaatg cacctaactg
     8881 agcttttctt ttttctccga tatccatctg tcccaaatac ttaaatcttg agatcagttg
     8941 ctgtccatct gccgaagaga ctttcttcca gaatgttcgt ttttttccat agcgaaaata
     9001 gatgtaaaat acttctgaag ctggcaatat atatacgtct tgggcatgtt tggcaaaact
     9061 ttcacttatc atttgatccg ataatcgttt tatttccatt attctcccct ccacaaaaaa
     9121 catacgtctt tttgatcttt ttgtttcaaa taaaatgaat ttacaaaaaa atattggcaa
     9181 aaaccgaatt tatgttatac ttataaaaat ttatcttggg aggcactcca ttggaaacaa
     9241 cattaaggaa atatatcgaa gtagcaagta aaaaaaagcc tgctgaccta gtgatcaagc
     9301 atgctaagat cgtcaacgtc tttacgaaag aaatcatgga aaaagatgta gcaatctgcg
     9361 aaggaatgat cgtaggaatc ggtgaatacg aaggaaaaag cgtacataat gctaatgggc
     9421 aatatctagt gccaggattc atcgatggcc atgtgcatat cgaaagctca cttttatcac
     9481 caagcgaatt ttcaaaagta tccttgcttc atggagttac tactgtagtg acagatcccc
     9541 atgaaatagg aaacattgct gggagtacag gtcttgagtt tatgatagaa gatgcaagac
     9601 aaacactcat gaatatcttt tttatgctac cttcttgtgt acctgtaacc ccttttgaga
     9661 ccaatggtgc aaatttaaat gcaaaaaaat tagccccttt tttacaaaaa cccgaagttt
     9721 taggcttagc tgaagtcatg aactatcaag cagtagcaaa taatgaaaca gacatcctag
     9781 aaaagatcca attgatgcac cagcataaga aaaaaattga cgggcatgca gctggtatcg
     9841 gcatggaaga gttaaatgtt tatcctgcag caggtattcg gacggatcat gaagctacaa
     9901 ctgcaaaaga agctaaagaa cgtttggatc ttggtatgta tttaatggtt cgagaaggga
     9961 ccgttgcaaa agatctggca tcgctactac cggcgatcac acctgaaaat tcaagaagat
    10021 gcttctttgt caccgatgat aaactgatca atgatttagt aaaagaagga tcaatcgatc
    10081 atatcgtccg tcaagctatt gcacttggaa tcgatccatt acaagccatt cagatggcaa
    10141 cattgaatgc agcagaatgt tttgatttga aaacactagg tgcgattgcc cctgggtatc
    10201 aagctgattt cttcttaaca gacgatctta ccaagctgcc aattcatgaa gttttctata
    10261 agggaacatt agttgttcaa tctgggcaac tccaagacca tttatttgct gatcattcaa
    10321 attcatatac gtgttgtctg ccaaaactaa acgctcaacc attaaatcca cattcgttag
    10381 agctgcttct ttcttcttca caagcccatg tgatcgaaat tatcccgaat agtctagtga
    10441 ctaaagattt ggtagaaaaa gtggaccgac aaggcgaacg attcattcct tcaatcgaaa
    10501 aagaccaatt aaagattgcg gtgatcgagc gtcaccatca aacgggaaac attggattag
    10561 gcatagtgaa aggatttcag ctgaaacaag gagcgatagc gacaacgatt gcccatgact
    10621 cgcataatct agttgtcgtt gggacaaacg acgaggatat gctgtatgct gctaatcttt
    10681 tgattcaaaa aggtggcggg atgaccgttg tcaacaatca gcaggaaata gcttgtgttt
    10741 ctcttcctat aggcggaatc atgacacagg aacctttttc gatcgtctat gaacagttga
    10801 atgaattaat aactaaagca catcaactag gagcagctaa aacctttgat ccttttttga
    10861 cactttcttt cttaacatta tctgtcatac ccgaattgaa gatcaccgac aaaggattat
    10921 tttcattttc tcgcttccaa ttgattccac cttgtggaaa aaccgatgaa ataaatacat
    10981 tttaaatcaa ataaatccca gaaaatacat tacatctgta atgttcattt tctgggattt
    11041 atttatggat attaatgttt ttatgaaagc agaaagttag tttcacagac ttttcttttt
    11101 ggtcttatct tgaaattgag cttctactcg ataaatacgt tgaccttttg aagaaaattt
    11161 ttcttcatac tccgtcataa tattgccttc aaattgactt tgatgtaaat ctaaccatac
    11221 ctgtttgagg atcatgccat attgagaaaa acttgccaga gaatactcaa acaatccttg
    11281 attatctgtt ttgaaatgga tttctccatt tggacgaaga atctgttcat ctacagcaag
    11341 aaaagtctta tatgtcaatc gccgtttttc atgacgtttt tttggccatg gatcggaaaa
    11401 attcagatag atttgatcta cttcgctgtc agcgaaatac tctgtcaacg ctgatccatc
    11461 cacatgtaga agtttcaggt taggaagcgg ttgttcgatc aacttgtcta gtgcaatcga
    11521 aacaacactg acctgcattt ctactccaat ataattgatt tcaggatggg cttttgccat
    11581 tccagtgatg aactggcctt tccccatacc aatctcaatg tggattggat gatcgttttc
    11641 aaacaattca ttccattttc ccttccacaa tgttggatca ctaattacaa aattcgggtg
    11701 agccgctaac atttccgctg ctcccggacg atttcggaca cgcattattt atctccattc
    11761 ctttcctcta attaaaaagg tctggccaaa tctccgcaga gtttgggcca aacctcagcc
    11821 actgaaaatt cgttttaatt gtaaaatttc ttcgttcatc cgtcgatgat cttcacgttt
    11881 gcaatatctg gtgatttctt ggagcaaatt catggccgca taccaatgaa atttttcaag
    11941 catttcgtca gtcgggcgta tgccataact gatcagccac tgattccagc tagacagtgg
    12001 cacataattt cctaaaatcg ttgcaatatc caccgcaggg tctgcaaaca tgattgaatc
    12061 ccaatcgact agatataaat aatttttaca tactaaccaa ttacgatggt ttacatctcc
    12121 gtgtactact gataagtttt ccttatctat tgacgggatc tgctcacgaa gaaaacgatg
    12181 gacaatttgc agaaattgat ttttctggag ttcttccggt aattcttttt catatttttg
    12241 cagcatcttc tccggcgtca tgacacgacc accgatacgg gtcagcatgg ttttaagaga
    12301 atttgaatga tgtaaatggt acaaaacatc aatcacgtcg tttctttgac caatctcatt
    12361 aggagtcagc aagcgacctt ccagccactc ttgtgctgtc aaagtatcgc cattccccgt
    12421 tcgttttgtc caaacaagtt ttggtgtaat tccttccctt gaaagagcag ccaacatcgg
    12481 agtcgtgttt cgtttaataa agactttgtc ctcatcacgc atgcctatgt atgtttttcc
    12541 tgtgtcgcct ttgatgggac gtaaccgcca ctcttggtct aactgaaact ccatcatgca
    12601 ttctcccgtc ctcttactct ttgttttgca gtacttatta ttctagtcgc ctgaacacta
    12661 cacgtcaagc acatatacgt tctttttcat ttaattgcca ttcaaaaaac cagcttttct
    12721 caaaaaaaat agaacgaaca gcattcttac tgtcaacgct ctatttttca ttctcatttt
    12781 taccctacac agctttgttt tcagtctgac atcttcttca aacgagttgg tacatatagt
    12841 ttaacaaaga gcatagtaac aatcaaataa gccgctagcg gaacccatcg ctgctgccca
    12901 tctaatacga ctgcagcaat caaaccaaaa ataaccgacg cgataattaa tacccatgtg
    12961 agtagatact gaatcgcctg cttcttctga ttcttatgga ttggataaag ctgaacgaga
    13021 accatatagc ggaactggtt cgctaaaggt aacagttgga aaccgatcaa atataaaaat
    13081 aagaaaccga taatcagact aaaataaaag tcgactactc cggcaatcaa taacccacct
    13141 ataattacta atcggaaata aagaccacta aaatcagaac ctcttgccaa tcttcttgca
    13201 aacaagtaaa gataagtgtt ttttgattct cttttaatat gagtcaatat aggatccaaa
    13261 tagcgtcttc ttttgatctt tgccgtaatt tcaggaacat ctgtgaataa attgataaat
    13321 tgatagatct gatgcaacct atgttcttct acatgcacca ttttctccca atccaatcct
    13381 ttttcttgtt gcttccacaa aaaccagtaa tatagacttg cctgtactaa tgcacctgct
    13441 attcctgccc atattagcca ataaaatgat agaaacaaaa agatcagaga agaaaaaaac
    13501 cagcctaatt tccaataacg aataacttgt tgatctagtc gaaacaattc tgctctcttg
    13561 atttgcagat gactgaattt caaactccat aatatgatca gatagacgaa aaacatcgaa
    13621 aaggattgct ccgtactaac caccactaat ggcatggcca atccgcatgc caatatcaaa
    13681 aagatcaacg gaaaaatgca agaatagcga aaagctcgtt ccaaataggt ttgcatttct
    13741 ttttctttag gaagtaaaaa aacaatatct gctgcctctg caaaagtagc catcttaccc
    13801 acgaataaac agagcatcca aaatacagag attatgagcc ctccaattgc aaacggtggc
    13861 tgcagggttt tcaaccaatt agaataatat aatcccacac cgccaatcaa gaaaagacaa
    13921 actaaaacaa aatgatcgtt caatacataa cgcatatatt tcatcatttt cttttgatgt
    13981 cttgctaaac gtttttgaaa aaaactactc atgtgcctgc tcctttgtta aagcaatata
    14041 gatttcgtcc aaagaagaat ccggcagctg aaattctttt ctcaacatct caagagaacc
    14101 gtctgctcgt atctctccgt tatgtaagac cacaaaacga tcacaatatt tttctgcagt
    14161 agctaaaata tgcgtagaca tcaaaatacc ggctccttct tttttcattt ctaccatcaa
    14221 ttcaagcaat gcattgatag ctagtggatc caagcctaaa aatggttcgt cgatgatata
    14281 taaagaaggc tcgatcaaaa aggcgcataa gatcatcact ttctgtttca ttccctttga
    14341 aaaattagct gggaaccagt caagacgatt agataaccga aaagtctcca acaacttttc
    14401 tgctcttttc attgctatat ccataggtat atcgtaggcc atcgcagtga cttcgatatg
    14461 ttctctcaat gtcaattctt catataaaga aggagtctct ggaatatacc caatttgttt
    14521 gcgatattct tccgtatttt tctttaagga tagatgattg atttctatcg ttccttttga
    14581 agcttccagc agaccgataa tgtgcttgat ggtcgtactt ttccctgctc cgttcagtcc
    14641 aatcaaccct actatctctc caggctgtac atcgaaactg atatctttta acacagggag
    14701 atgtccgtac ccccctgtga catgatcgat aattaaactc attctttttc ctccgccatt
    14761 tatttttatt tattatacca cgaagaaaat gacggtattt tgtttttttc cttacaatag
    14821 ctttaactta tggtaaagtt aagctgaagt atttattctt tatagaaagg atgttttata
    14881 tggaaaactg tattttttgc aaaatcatca atcaagaaat cccaagctat aaaatatatg
    14941 aagacgacaa ggtctatgct tttttagaca tctctcaagc aacgaaagga cacacattag
    15001 ttgtgcctaa acgtcatgta gcagatattt ttgaatatga tcccgaactt gcaggagagg
    15061 ttttttcgcg tgtgcctaag attgctcaag cattggaaaa agcttttcca gaaatgaaag
    15121 gtcttaatat cttgaataac aaccgtgaac tagcctatca atctgttttt cattcacata
    15181 tccatttagt ccctcgctac agcaaagagg atgatttttc tattcacttt gtcaatcatc
    15241 aagatagtta tggatcggaa gaactaaaag ctattcaaga aacaatcgta aagcaggtgt
    15301 cctgcgatga ttaaaaactt tttcaaagga ctgttatttg gagctgctgc tggcactgtc
    15361 ggaggtctgt tattggcgcc caggagcggg aatgaaacaa gaaagaaact gatcgaagaa
    15421 ttggaagaag caacaactct gacaaatgat ttaaataaca gcctgaatca ttttaaaagc
    15481 gcattgatcg aaacaaaaga aacagctgaa tctgtgattc ctgctttcca agaagcaatc
    15541 aaaaaagata tcgaagattt taaattccaa gccgaaccac gtatcacaca aatcaatgaa
    15601 caagtagaaa aactaaaagc tgatctgcca gagtatcctg aacaaatata ataatgatta
    15661 ttttctcaac agatttaact atataagtga gaaaagtttg cgtatttcct aggaatgtga
    15721 tgaaattttg aattttgttt gaaactacca cgacaacgtt tttaaaggaa atgggatatg
    15781 gtatagttaa tatataaaaa taataactaa ttacaggagt gcaaatttta atgaaaaaaa
    15841 aatcaattat tttggcagct actagtgcgt tggctgtttt aactctagca gcttgttcag
    15901 gcgatactaa taaagatatc gcaacgatga aaggcggaac gatcactgta tctgatttct
    15961 atgatgaagc aaaacttgaa tcatcgaacc aatccttagt acaacggatg atcatttata
    16021 aagtattcaa taacaaatat ggcgataaag taaccgataa gcaagtggat gcagaatacg
    16081 acaaacaagc gaaatcatta ggtgatactt ttgaatcaca attagaagct gctggatata
    16141 cgaaagatac ttataaagaa tatattcgta acaatttagc ttttgaagct ggcttgaaag
    16201 ctcacgtaga tattacagat gacgatttga aaactgcttg gaaatctttc catccagaag
    16261 tagaagcaca aatcatcaaa ttatcaagtg aagatgaagc aaaagatgtg aaaaaatcag
    16321 cggatgatgg agatgatttc tctaaattag ccaaagacaa atcaactgat acagagacaa
    16381 aagaagatgg cggtaaagtt aaattcgact caacgacaac aactattcct gcagaagtaa
    16441 aagaagcagc tttcaaattg aaagacggtg agatctccga tgttattact acaacaaatc
    16501 ctacttctta tgcgacagaa tactacgttg tcaaaatggt gaaaaaccaa aataaaggca
    16561 atgacatgga taaatacaaa gatcaactaa aagatatcgc tactgaaact aaactaagtg
    16621 acaatgcatt cacaactaaa gtaatcggtg aagaattaaa agatgcaaat gtaaaaatca
    16681 aagatgatgc ctttgaaaat gtgttgagtg cattcacaac aacttcatca agtacgaaag
    16741 actcaagtga aactacagct tcaacaaaga gttctgacac aaaatcaact gattcaacaa
    16801 aagaatcttc aactgaagaa acaactgatt catcaaaata aaa
//
LOCUS       DADVXO010000060        16716 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.062, whole genome shotgun sequence.
ACCESSION   DADVXO010000060 DADVXO010000000
VERSION     DADVXO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.062"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(253..1464)
                     /locus_tag="KB950_001623"
     CDS             complement(253..1464)
                     /locus_tag="KB950_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6857844.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1583..2218)
                     /locus_tag="KB950_001624"
     CDS             complement(1583..2218)
                     /locus_tag="KB950_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBB6857845.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2354..3007)
                     /locus_tag="KB950_001625"
     CDS             complement(2354..3007)
                     /locus_tag="KB950_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857846.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3110..3316)
                     /locus_tag="KB950_001626"
     CDS             complement(3110..3316)
                     /locus_tag="KB950_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBB6857847.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3392..4138)
                     /locus_tag="KB950_001627"
     CDS             complement(3392..4138)
                     /locus_tag="KB950_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857848.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4298..4522
                     /locus_tag="KB950_001628"
     CDS             4298..4522
                     /locus_tag="KB950_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBB6857849.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4546..5307
                     /locus_tag="KB950_001629"
     CDS             4546..5307
                     /locus_tag="KB950_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBB6857850.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5319..5576
                     /locus_tag="KB950_001630"
     CDS             5319..5576
                     /locus_tag="KB950_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857851.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5668..5919
                     /locus_tag="KB950_001631"
     CDS             5668..5919
                     /locus_tag="KB950_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857852.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5895..6068
                     /locus_tag="KB950_001632"
     CDS             5895..6068
                     /locus_tag="KB950_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857853.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6182..6589)
                     /locus_tag="KB950_001633"
     CDS             complement(6182..6589)
                     /locus_tag="KB950_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857854.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6750..6974
                     /locus_tag="KB950_001634"
     CDS             6750..6974
                     /locus_tag="KB950_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857855.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6984..7178
                     /locus_tag="KB950_001635"
     CDS             6984..7178
                     /locus_tag="KB950_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857856.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7181..8203
                     /locus_tag="KB950_001636"
     CDS             7181..8203
                     /locus_tag="KB950_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBB6857857.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8166..8981
                     /locus_tag="KB950_001637"
     CDS             8166..8981
                     /locus_tag="KB950_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBB6857858.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8999..9781
                     /locus_tag="KB950_001638"
     CDS             8999..9781
                     /locus_tag="KB950_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857859.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9781..10629
                     /locus_tag="KB950_001639"
     CDS             9781..10629
                     /locus_tag="KB950_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6857860.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10626..10868
                     /locus_tag="KB950_001640"
     CDS             10626..10868
                     /locus_tag="KB950_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857861.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11011..11379
                     /locus_tag="KB950_001641"
     CDS             11011..11379
                     /locus_tag="KB950_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBB6857862.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11394..11555
                     /locus_tag="KB950_001642"
     CDS             11394..11555
                     /locus_tag="KB950_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6857863.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11552..11878
                     /locus_tag="KB950_001643"
     CDS             11552..11878
                     /locus_tag="KB950_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857864.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11875..12690
                     /locus_tag="KB950_001644"
     CDS             11875..12690
                     /locus_tag="KB950_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBB6857865.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12829..13140
                     /locus_tag="KB950_001645"
     CDS             12829..13140
                     /locus_tag="KB950_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBB6857866.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13164..13382
                     /locus_tag="KB950_001646"
     CDS             13164..13382
                     /locus_tag="KB950_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857867.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13379..13795
                     /locus_tag="KB950_001647"
     CDS             13379..13795
                     /locus_tag="KB950_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBB6857868.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13792..14130
                     /locus_tag="KB950_001648"
     CDS             13792..14130
                     /locus_tag="KB950_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857869.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14127..14357
                     /locus_tag="KB950_001649"
     CDS             14127..14357
                     /locus_tag="KB950_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857870.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14364..14567
                     /locus_tag="KB950_001650"
     CDS             14364..14567
                     /locus_tag="KB950_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBB6857871.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14564..14860
                     /locus_tag="KB950_001651"
     CDS             14564..14860
                     /locus_tag="KB950_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857872.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14937..15350
                     /locus_tag="KB950_001652"
     CDS             14937..15350
                     /locus_tag="KB950_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBB6857873.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15363..15749
                     /locus_tag="KB950_001653"
     CDS             15363..15749
                     /locus_tag="KB950_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857874.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16300..16527
                     /locus_tag="KB950_001654"
     CDS             16300..16527
                     /locus_tag="KB950_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857875.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16524..>16716
                     /locus_tag="KB950_001655"
     CDS             16524..>16716
                     /locus_tag="KB950_001655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857876.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5666 a         2726 c         3485 g         4839 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 ttcgttattt aatggtattt taaatgaaga taagcgaatt tacagcattt ttaaatgctt
      181 atataccgca aattatccca agtttttgta tatcctaccc gagtttatac ccgaaaatat
      241 aattttttag tgttatttta tatctttttt taataggttt tcaaacgctg tagttgctgt
      301 atctttttct ttgcgtaaga catggccata aacattcatt gtagtattaa aatctcggtg
      361 tcccaatcgt ttttgaagct cttttggagt catattagga tcgctaagca aataggtagc
      421 tgatgtatgt ctaatgtcgt gaaaacgaat gtgacgcaaa ttgtgacgtt tggtgaatcg
      481 tttgaacttc ttatacagat aacttccacg tggcaaagtt ccatcttgtt ttgtatcaaa
      541 tataaaatag tgttttaatt tctttatatt taatttccaa cgaatctttt tattctcttt
      601 gattaattca tctaacatat caaatagata agcaggaccg gttactattt tttcatcatc
      661 atcatttttc aaacctggca gcaaccgaat atctgatttt ccatccactt ctgaaatccg
      721 ctgatgaaat cttatttctt gtttgtcaaa gtcaatatct ttttcttcta aagctgcaat
      781 ttctccttgt cttgctcctg taataaatgc tagaacaatt aaagttttaa tttctatgct
      841 ttcctcaaat gctgctttta gcattgcatg aatttcttct tccgaataag gttcttcgat
      901 attctttttt acgccttttt cttttggtat ttttacattt tccacaggat tttctttaat
      961 tgctccgtac tcatttttag ccatcaaaaa taagttgtta acggcgtata acatacgaag
     1021 ctttgtattt cttgataaag gtttatcaga gtttctccca ggatcttttg tattgattct
     1081 ttgagcatta acaacaattt tctttatcat gtatggtttg atatcgatta gagggataaa
     1141 atcaaattta tcaagaaatc tattctcgat cattcgctta tagttatgat aagactttgg
     1201 ctctaaattt ttttcggcct ctgaaagcca tatttttcta taaaattcac caaaagttat
     1261 attgaacaaa tccaacgaat catcggtcat tccttcgaat tgctctaacc agatgttcaa
     1321 ttctttatat gccgctcttt tactacttgc ttttatagtt ttgctttttc tttttgccga
     1381 gccatcaggt ttgtatccta atacagctct tagtctatat gtttcgttgt ctacttgttc
     1441 taagtatcct gttacttctg acataattga atcagctctc tttctctgat acaataggta
     1501 ctataaagaa gcctatcgta taggtttgtt tctttcttag aacacgctcg ctttggtcgg
     1561 tggggcgtgt tttttatact aattagttgt gaagagcaat tgaaactgtc tctgttccaa
     1621 agtaacctgt ttgtgcttgc aatacattat tttttgcatt tgcttgggct tcagagatat
     1681 caaaaactat tttaccagtt tgttgcatat caggatttaa gctttccata aagaaagagt
     1741 tagtgatatt accattttca tcttgatttg ccgacataga tgcagccgaa tcagcttcga
     1801 aagtttttcc atctgctttt aatttgaaga atgagctatc aacagtcaca gccttatcac
     1861 ctgcattttt tacagataag tcaactacta agaaagtgtc ttttgcgtta gtaggaagta
     1921 cagatgggcc aacttgtttt actacttcaa cggagtttac tttgtattcc attttcccta
     1981 cagcaacgtc atcaccgatt ttataagtct tttcttcttt tgttgtagtt tcttttgtag
     2041 tttctttatt agtacttgtt gatgttgcag tagctgaatc tttgttttca ccgccactta
     2101 gagcaccacc aataccaaaa aacaaaagta ctattaatac ccaaaaccat acccgtttat
     2161 aaaacggttt tttaacctta tacattttcc catcttgacc cataactttt tttgccattt
     2221 aaatattcct catttcttgt tataatatat ttgtgatctc agaaatgagg tatgagtccg
     2281 tgttgcagca cgggcttttt tatttataac ttttttagag attataggca aaatagtagg
     2341 gcataaaaat atattattga attccgtatt taaaaaatcc taattgaact tcaccggaag
     2401 tcttttgctg tgttgtacgc aatgcttctt cttcagacat tccattctgt actttccatg
     2461 caacaggcga catcccgtat ttgttaacaa aatcagtaag tgataaagtg tcagcgtctt
     2521 gctgagcgct tgtttgttgg tcttctggat tttgttgaga tgctgcttgt tgttctttct
     2581 gatcttgact gataatattg ccagcatcat ctgtagtcaa tccattttca taaagggcca
     2641 cgccgaaagc ttcccactct ttgttggacc aatttgcacg atcagctgga gttgactgta
     2701 aagtgcgttg tttcatctgt tcatatgttt cttcttgagg tgcggtttgg attgtactct
     2761 gactggagct tataactgtt gggctaggtt ccgccgtaac ttggttggag cttgtaactg
     2821 ttgaactaga atctgtttta gatgtagatt tgctagtaga tgaactggtt tcagttgttt
     2881 cttttgtttt acttattttt gtttcttggt tagaagtggc atctgttgat tcagcttttt
     2941 tattatttga acaagctgaa agtagcagag cagtacttaa caacaacata acgctaactt
     3001 ttttcatttt atactcctct tttctttgat acaataagtt gtaaagaagc ctactatagg
     3061 tttaacatcc cacgtccaca aactttggtc ggtgggcgtg tttttttgtt tatagacttt
     3121 cgaaaactaa agtagcttgg attctatcgc caccaccgaa acctttacta ccaccgtttg
     3181 tagtggaaat agtgtgtaat cgataacctt tagaacactg tctatttata gtgttttcaa
     3241 gttctgatag gttttgcgac ccttttccta taaacttttc ttttaataca acttgcaata
     3301 caacgtaact tggcatattc ttttccttct ttctactatg ataataaata ctaaccccta
     3361 gccggagcgc caactacgac tggggtattt tttagaatac gactttcccg attattctaa
     3421 tctgttccgg tgagacgact ctatcttcat ataattcgtt tatagagcgt agaataatcg
     3481 tttgactctc ataatcctta tatacttctt tacacgtcac accgtcacca tctatttcaa
     3541 cgatagcaat ctcaccgtct tccacgtctg gctgttcctt aacaaataca aattgtccat
     3601 tcctgataac tggttccatt gaatcgccta taataggaac agcaaaatca gcattgtttg
     3661 gaactctttc aaattcttct tcctctacaa ctgtatcgcc ataaactaat tcagtcgggt
     3721 tggcggcaga agctgaattt ttgattgtcg gaagtttttc ctttttagga aagtgcacca
     3781 cgtttcctcg attttgctct tcgagttgct gttcagcaaa gttgtagact tttgtttgtc
     3841 ttggtttttc taattgattg tagattgttt caatggatgg aatgttattt ttcttatctt
     3901 ctttaggaac atcgtaacca agcaaccatg cctcactcac atttaaagtt ttagccaaca
     3961 aataaagctt gtgttggtcg ggcgatgact taccattgac atattgcgat agatgacttt
     4021 ttgacatttt tattccagtt tctttttgca aaggtaaaga catattcaaa atatcaactt
     4081 gctttaaatt tctttcagac attaactgat taagtcgttg ctgagtcgtt tgcttcatct
     4141 tatacctcct ttctgatact tattatatac cttattgaac ataagttcaa taaaaaagtt
     4201 aaattaattt gaacttttat gttgacaatg ttttatgcag gagttatact ttggttacaa
     4261 agttcaaaac atttgaacta gaaaggagcg aactaatatg agttttgatt attctaagtt
     4321 gtcgggaaga attgttgaga aatatggaac gcaatacaat ttttcaatcg ctttaggttt
     4381 gtcggaaaga agtttgtcat taaagttgaa taaccgagta ccatggaaaa gcaccgaact
     4441 tcaaaaagcc attgaattgc tagatattcc aaaagaagaa attggcgaat atttttttaa
     4501 tttagaagtt caaaatgtct gaacttaaga aaggaattcg aaaaaatgaa cacacaaaaa
     4561 tttgaaaaca atctatttga attagaagtt aagacggaaa atggtgaatc gttatttaat
     4621 gctgaaacag tagcaagaag tttgggtttc gtggaaacaa aaaacggtaa agaatatgtt
     4681 cggtggagaa ctgtcaatgg atatttaaaa aaataccttt cgcaagaagt tgcgaaaaac
     4741 gactttatca gtgagccaat ggtttacaaa ttagccttta aagcaaacaa cgtacttgct
     4801 gaaaaattcc aagactggtt agcaacggaa gtcctacctc aaatccgaaa acacggcatg
     4861 tacgcaacag atgaactact taacaatcca gacttgctga ttgaagtagc aaccaaactc
     4921 aaagaagaac gcacgttacg attgatagct gaacaacgag taaatgaatt acaaccgaag
     4981 gcagactatt acgaccgaat cttgaacaac aaaggattag ttacagttag tacgattgct
     5041 aagaactacg gtatgagcgc tgtatcgttt aataaattgc tacatgaatt aggtatccag
     5101 ttcaatcaaa gtggcacatg gctgctatac agtaagttcc aagacaaagg atacacgcat
     5161 attgagccat ttgattatga agataaaaac ggcaatcggc aagtaaaaac aaggatgaaa
     5221 tggacgcaaa aaggacacat cttcttatat gaaacattga agaagaataa ttacttaccg
     5281 atgattgaac gtgaacaaac agcataggag gtaacactat gaacgaggaa atgagccaaa
     5341 caatcaaggt acaaaaaatg atagatgatc taacacatgc aattgatagc caagccgata
     5401 aaatcatcaa ggaattgcaa ggacaaaaag tttgggatgc aaaaatgctt ttcaagacta
     5461 tcaaatttga aatgaatcca acaaaaagaa agctcgccga tgtgttggaa gaaaagttgg
     5521 cttccgctat caacgagcaa gaattactat ttgaaaccga tacttttaac agctgatgtt
     5581 ttatagatga cagtttcatc cgaatctata tcattataga ttcgattatt gcaatgtacg
     5641 aattataaat acaggaggaa ataaccaatg gagcaactag ctttagttaa cctctcagac
     5701 cttaaagctt ctttggcaga atctgagatt gcaaatgaag tgtgggatac aaagcaagct
     5761 gcggaatatt taaaaaccac aacacgcacg ctgactaaag atgccgaatc tggaaagatt
     5821 ccagcagcga aagttggcag agagtggagg tttagtagta tcgctttgta tcagtatcta
     5881 aaaggaggaa aaacatgggt aaattcaaca gagcactagt gttcagcgca cccctaatcg
     5941 tctatgcttt aggactttgg ggaagcagac aagcattaat cggcacgatc gtttacatgg
     6001 tttggatttt tatggggctt gatgaagctg agtacagagc gaaaaagccc accgaggggg
     6061 ctgactaaag tgtggttctg tttattaggt gtttatctcg tggccgtttt agtaaataac
     6121 tacatgagaa aacgtggcga atactggtat acatcctatg cggttctagt atgtctaatg
     6181 cttacaattt ttctaatgat ttattcaaaa taggtaatac tttgtcaatc aaataagttt
     6241 caagagattt ttgttctgca tcagtgttgc cgtaaccaaa acggcttaag aaaattttca
     6301 taacctctat atcttcattt tgaatatatg gcaggactgc ataacctgat gcttttacag
     6361 cagatatatt tttatcagtg ttactaccaa tacaaatacc cacattgttg agaaatgttg
     6421 agaaagttgt ttgtattgtt tgggtttttg aatcgtgttt ttgtaattca atttctttat
     6481 ccttcatcga ttcaacatgc ttattattta taaccgctgt aatcgaaggg gatataagag
     6541 ctactaaagc taggataatc gaaattgtga tcgtgttatc gaaactcatt ttttcaccat
     6601 ccagttttta aaccattata tcaaaaagga gagaagaaat aatgcaagaa ttagtaattt
     6661 tgaaaaataa aaaaagacac gaacgccagc aagcatgatc gtgtccaaaa gaaaaatctt
     6721 ttaaaccatt ataacacagg aggaaataaa tgatctcagt taaagggtta ggcgatgaaa
     6781 tattcgaagc aatgatgagc aaagcacaac aggacatcaa agaaaaaatc atttctgctg
     6841 cagcgcaagg acaaactagc tgcactgttc gatcgagagg aacgacacca agttttcttt
     6901 tggcgctaga agatgaaggc attttgaata tcaaacgaag agatggcagc gtcagattgt
     6961 tttggcaatt ttaggaggcc gtgatgaaag attttgattc tttaggcgcc agacagcaac
     7021 caccaaatga agctagtcct gttggagttg attggcaaga aaatccgtta tatccaggtg
     7081 atacttgcta tctaacagaa gaaggctatg taccagtaga cgcaattcta gagtacgtcc
     7141 agcaacatta tccaaagatt gaattaggag gaatttagga atggcaaatg atttaacaca
     7201 aacaacacaa cgttcattag atgagcaggt aatcagtaat cttggacggt tacaagaaca
     7261 aggtttagaa atgccaccag gatatagtcc acaaaatgcg ttaaaaagtg cttttttcga
     7321 actcactaac aatacaggag gaaacttgtt gcagatggct gcaaacaatc aagaaatgaa
     7381 aacctccatt tctaatgcac ttttagacat ggttattcaa gggttatctc cagcaaaaaa
     7441 acaatgctat ttcattaaat acggaaacaa ggttcagcta atgcgttcat attttggaac
     7501 aatggcggtc cttgatcgtg taactggagg ggcagatatt acgcctgtag tcgttcgaca
     7561 aggtgatgag tttgaagttg caatggatgg accaaacatg gttgtcaaaa agcatgaaac
     7621 aaaattcgag aatttagaca atgaaatcat tgcagcctat gtagtcatca agttaacaaa
     7681 tggtaaagag accacaacag tcatgacgaa aaagcaaatc gatcaaagtt gggctaagtc
     7741 aaaaatgaaa gattctggac cgcaaaagga atttccagaa gaaatggcta aacggacagt
     7801 aatcaatcgg gcagctaaaa cattgatcaa cacaagcaac gacaacgatt tattagttca
     7861 agctgcaaaa gatacgttag aaaatgaatt tgacaatgat cggaaagata taacgccaca
     7921 aacagaaaaa gtggctactc tcgaacaaaa attcttttca aacaaaaaga ttactgaacc
     7981 aatacaaaaa gaaccagatc cgattgtgat tccagatgat atccaaaatg aagtaactcg
     8041 agttgctgat gtacctgccc atccagaaat agagcaagca catttgatcg aaaatgagga
     8101 tatcaatcca attcaagaag aattgataga tattccagac tttggacgcg aggaaggtgt
     8161 agaagatgtc tcagaatttg aagacgacga gtaccctttc tgatgagaat tattactcca
     8221 atgaagctga ctggcagtac atgtcaacat cacaatataa gtccttttta aaatgtgaag
     8281 ccgcagcctt agcaaagttg aaaggcgatt gggcaccgac gtcggatccc aaagccttac
     8341 tcgtaggaaa ctacgtacat tcttattttg aatcaaaaga agtacatgaa gcgttcaaag
     8401 aagaaaataa atccaaaatg ttttctagta ggaagccgta tggattgttg aaagatttcc
     8461 aaattgctga acaaatgatt gaacgactta agcaagaaga agcattcatg aacatttatc
     8521 aaggtgagaa agaaacgatc gtgactgggg aactatttgg aacgacatgg aaaggcaaga
     8581 ttgattgctt gaatgttgag gatgagtatt ttgttgatat caaaacaacc aaagacatgc
     8641 atgagcggaa atggaacgaa aactacggat caagagaaac gttcattgtc aattttggtt
     8701 acgtacttca aatggcaatc tatcaagagc ttcttttaca gcaatacgga aagatgttca
     8761 tgccaatcat cgctgcggta tcgaagcaaa cgcctagtga ggcgaagttg ataacgattg
     8821 atcaagataa gatgagctac gagctaatca tgctaaaaga aaatattgaa agaattgtca
     8881 aagtgaaaaa tggcgaagaa aggccgatac cttgcggtac gtgcgagtat tgtcgaggta
     8941 acctttcaat tactggattc actagcatgg acgatcttta aaaggaggtg ctttgcgaat
     9001 gaatctagga tacatcaagt tataccgtaa agtgactagt tcattcgttt ggaccaattc
     9061 cgatatgttc aagctatgga tattgtgttt aatgaaggct agtcatgaag atagaaaatt
     9121 tttatttaac ggtcaagaag tacgtttgac aagcggacaa ttcgtcacag gagcccatgc
     9181 aatagcaaaa gagtacaacg aaggagtccc gagtgacaaa gcaattgcat ggcgaacgct
     9241 atggagatgg cttaagaaat ttgaaaatga agaattattg acaatccaat cgaacgctag
     9301 atatagcgtt ataacaataa taaattggtc tgactatcaa tcgagtgaca agccagtgac
     9361 aagcgacgga caatcgagtg acaagccagt gacaacaaac aagaatgata agaatgataa
     9421 gaatgataag aataataatc ctcgcaactc tcgaaaaaca cgagagtatg cagatgacga
     9481 tccaaataaa aaattggcca ttcttttatt aaaactcatt cgaaagaatc gtagtctcaa
     9541 agaacccgat ttgaacaaat gggccaatac gattcgtttg acgattgaag ctgacaaacg
     9601 aactggaaaa gaagttcaag acatgattgt ttgggctact aaccatgatt tttggtccac
     9661 agtcatttca tcgccaacta gcttaagaaa aaatttcgat acaatggtgg gtcaaaaaaa
     9721 taaaagaaag caacaaaata tttctaatga tgagttacca gaaacaggtg aggattggta
     9781 atgaataaaa aactaagtgc aatggctgca ccttacggcg gattaaaaac agcagatcat
     9841 aattgcccga aatgtggtga tccattgtat atctggaaaa caaaaaataa agatggtact
     9901 gatcgatgcg gtcctacatg tatcaataag atttgtggtt atcgagaaat ggtaactaaa
     9961 aatcaaaaag aagctatcca aaaagcgaat gaagcaatga aaaaggacgc tatcaatcga
    10021 atgcttaaca gttcaatgat tacagatgat gccatatgga ccttcgattt tgatggatac
    10081 aaagtagttg atcaggaaac agcacaaata aaagcgatgg ctcaagaatg ggctaaaaaa
    10141 attgtaaatg gtagcacgat tcatgcagtg ataaccggta gaacaggagc cggcaaaacc
    10201 catctaggcg ctgcagtgat taaagaagtc atgagggcat ctaattataa aattgcttgt
    10261 tcatttatca gttatcgaga acttttagaa cagttgaaat ttgcaatgaa tgatccagaa
    10321 gcaagaaaag ccgtaacagg ttcgctgatg gctgagataa agaaatcaga tttcgtggta
    10381 atcgacgatt tgggtgcaga actaggaaga atggaagaaa acaaccaggc aacgccttat
    10441 gatgttgatg ttctcacatc gctcacagaa gctcgtctaa acaaagctac gatattcaca
    10501 accaatttat catcgaagca attaaaacac gcgtacggtg agcgagtgtt ctctcgtgta
    10561 atgaatggaa cgaaagggaa catagctgta ttcaaaacaa cgacagacaa aaggaggaat
    10621 ccagtttgac ctttgtagtg aaaaaaatgt gctacttaga tagccgaggg cgaggagaag
    10681 ctagtgtcga gtttgctaaa caccacacga ctaaagaaga ggctgacctt gttgcaagtg
    10741 tttgtggcgg cgaagtagtc gaagttatta aacccaaaag acgattttca aaacctaaga
    10801 caaagccagt gaaaaaagag tgttgttgtc cgaaaagtaa ccaagcatgg atgagaggtg
    10861 caaaatgact tgtttaaaat gcaacgatga aacagttatt tggtataaga cattgcttgg
    10921 atggtcaact tgtgaacctt gtccaatatg caatgaaaat ggacgacgtt cgaaagaacg
    10981 actcgaaaga ctaaagaagg agtatagcaa atggcaacaa gaagcaaata cggaaacaaa
    11041 aagcacgaag tagacggcat cacttttgat tctatcacgg aagcagagta ttacgatcta
    11101 gccttgtggc aagcacaggc gaatggttgg aaagtgaagc tccaggaaag gtttgagctg
    11161 atgccgaagt ttgaactaga gggcaagaag tatcgcaaga tcgagtatat tcccgacttc
    11221 acattttaca aaagcggcaa gctggtcaaa gtcgtagatg ttaaggggat gcagacaaaa
    11281 gactttaaga tcaaggcaaa gttgttctgt catcaatatc aagtgccgtt gatattagcc
    11341 aaaaaatatc ggaatacgtt caaggaagag cgtttttaac gaggtggtcc atcatgacaa
    11401 cagaagaagt gattcaaatg cgaattcgaa gccttcggcg tgagattgac gatctggaac
    11461 gaacaaaggc agtgatggtc aatgaaacgg ctagaaaggc aatcgatttg cacatagaga
    11521 atttaagaag ggaaatccgt agattggagg aatgagcgtg gataaggaag cggcttggcg
    11581 aaaattaatg ttgctgattc aagatgagaa ctggcaagaa gatgaagcag tggttgctga
    11641 agttcagcgt ctaggaaaga ttgctaacgg acgtatacga aaaaagccag acaaaagaaa
    11701 acagcgcaaa gggaaaatcg tcgttgtttt acacgaaggc aaaattttga tgcaaggaac
    11761 agctagtgag ctgtctgcag aaactggata tacgcgtggg actattcgaa cgtacgcttg
    11821 gcgaaatcac accgatcgaa aagggcacga atataagtat ttggaggaag aaaaatgaac
    11881 gaaaacaaat taatcaaatt gggtgtagca ggagcagtaa tcgtaggtat tggagttatc
    11941 ggaggattta agttcttcga aaaaatcgat aatggatatg tgggtgtgcg ctattcaatg
    12001 aacggcggta tcaaagatga agcactgacg caaggtgtga aatttgtagg gattgacaaa
    12061 gtgatccaat atccaattcg cttgcaaact atccaatcaa aaaatatttc agtatctaca
    12121 agcgacggca aaaagacaac gattgatatc aaatatgact acaaagttga ttcaactaaa
    12181 gcagcaaaaa tgtacaagga atttgggaat atcacttcgg aagatatcga aagtggatgg
    12241 ttaaaatcta agcttcaaaa ggtcgctcgt gaagtttatg cgaaatatag tctgcttgat
    12301 gtcctttcag gagattcctc taaagttgaa gctgaggtat taacgaactt tgctaaatca
    12361 gttgaatcta aagggtttga agtcgaagac gtaacacttg gtgttccaga tgtcgataaa
    12421 gaaacacaaa aatcaatcga tgcgatcatt cgagctggtc aagaaaatga aaaagcgaag
    12481 ctagacgcag aaactgcaaa aactcaagct gatagtgaag cttacaagaa aacaaaagct
    12541 gcagaagcag aggcagaatc taatcgcaaa gtcgccgaat cagtaacaga caatttgatt
    12601 cgttatgaag aagctcaagc tcgcaaaaag catggatggg taacagtaaa tggagcagat
    12661 actgtagtta cggatgaagc aggcaaataa tatgggattc tttatggcta aaattctctt
    12721 gttcttaggt ttagtaggag cagcatatct cgtgtatgcg ctcctttccc aaactgatga
    12781 caaagaagat gacaacaacg atgaaatgaa attttaggag gagaaataat ggacgaacta
    12841 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcaga tccaaaagca
    12901 cagtttttga aagtagctga ggaattcggg gaaatagctt cggcgatggc aagaagtaat
    12961 gatgaactat ttaaagatag cgtaggagac gttatcgtca cgctgattat cctttccatg
    13021 caaaaaggga caaacgtaca agagtgttta gaaatggcat acaacgaaat caaaggacgc
    13081 acagggaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggaggg cagcaaatga
    13141 taaaaaagct cattcaattc agcatggatt tatatgatat cgaatcagga gcaacactat
    13201 ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt attttgtggg
    13261 tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca aaagcggttg
    13321 agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag gacagcgaat
    13381 gataccgaag tttagagcgt gggataaacg aaagaacgta atgagagatg tagccgtctt
    13441 gcattttact aaaaacggca aaacaaactt tattgaatat tggataaatc ctaccgaatt
    13501 gaaatcatat cacgtgcgaa acatcgacct catgcaatcc acaggaatga aagataagaa
    13561 tggtgtggat atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta
    13621 cttagataat aaagtttgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc
    13681 cactgttgat gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac
    13741 gtatgaagtc atcggaaata tatacgagaa tagcgagtta ttggaggtag agtgattatc
    13801 ttagctactg actacatcga ttctttaaaa gatgaaaatg ggaatgtccc agcattgcta
    13861 aattgtggaa ttaaaagcaa gaatagaatc attttgttac ggatatcggc tgatttagca
    13921 aaatatcttt atctgagcaa gacagcagta tattacactg cggctacacg ttatcagtac
    13981 aagggaaaaa caatcagtca ggattattat gatcgtttaa tcagtcctga tatgtatggg
    14041 aattcagaat cagcaattaa gctgtttggc gcaatagaga tatatgccga tgattttcca
    14101 aatttatggt tgaaggagga agcggaatga aatacgaaat accactaagt gaagcagacg
    14161 tccagtcaat tattaacggt cgggaggtta ataaaaaact tcctgatggt actgaattag
    14221 tcatcagaca aagttatttg aaagatatgg cagctccagt attaattgat cgttttaacg
    14281 tgactgattc tgtggtagag aaccacttga aagaatttcg atcaagtata aacgacactt
    14341 tcagattagg gagttgattg acaatgaaca ccagacaccg cagagtagca aaactaagaa
    14401 aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag
    14461 aaacatataa agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg
    14521 gaatttcgat attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg
    14581 attttacgct agcagtcacg gtatcggcgg tttaatgcta gatatgccga caaagaatcc
    14641 taaaacacgt aagaaaccaa aagtcaaagt cggtgacatg gtccgctgtg aagcagagga
    14701 gttcatttat ccatttcgtg gatatgtaga acacgtctat aatcactcag cgatcattcg
    14761 cattgaaaac acgatggaat gtgataaatg gacagctaaa agcaaagaga atttagcagt
    14821 agtgagattg gtggatatgg aactaatcaa tgacaaataa aaaagccgga tcgctccgac
    14881 taacataata aaacagacaa gtttattata tcacataaaa ggagcagttt gaattgatgc
    14941 aattgttacg agaggtagat ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga
    15001 actttcggcg tttggagcgg atggcaggtc gctctttgat agatattaag tcgccgatta
    15061 ttacggatat gccgaaggca ccgaagcacg gcaataaggc agaggatgca ttgattcaga
    15121 tgatggatat agaagcggag agagacgcga ttttagcggc tttgatggct cttagtctga
    15181 ttagtcgtca gatactctac tacagttttt gtgtgccaga cagcttctca aactacagaa
    15241 ttagccgtga agtgggttat tcagaaagaa gtatacaacg gatgaaatcg gaagctctaa
    15301 tagagtttgc agaagcatat aaacacggaa gaataattgc ttataaatag ggggaccata
    15361 taatgtggaa agattatgta tcgttgaaag agttgaaaaa agatcttgtt ttcaaaagaa
    15421 ttgtagaatg gtcagagagt gaattaattt tagaagatgg aaccaaaatg gaagttgtat
    15481 gtagcgaatc agattgctgc gcatgggccg aaggtgaatt caaaaatgta aagcttgatg
    15541 cagtgattac agatataaag atttttgata aaggtaaccg tctctataat ggtgacggac
    15601 atacctctta tgctgaagtc gttgtttatc ataatcgtaa tgagatcgct aaagcagaat
    15661 gcacagcgaa tgatgggaat ggcggctact attatagtgt ttgtgcttta aaggtcaaag
    15721 acaaactttg tatagtgact gacgcataaa aaaatggcgg ttttttggcg gaatgatggc
    15781 ggtttttagc catttatcag tgatattatg gtagtgtcga aagattagtg ataggtctga
    15841 gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag
    15901 acagcaacgg aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta
    15961 ttatctatca tccgttgctg tttattgtta catacagatc gcttaggcgg tctttttatt
    16021 ttggccgaaa acctacattt tcgatggccg attattggag gaggaatagc atgttcaaac
    16081 tatctgaaat cattaagaag tccgacgttg agaaattaga gatgttaaaa aagaagttga
    16141 agaaatagca gtctcttcgt gaggctgttt tattttgctc acaaaaatag accactatcg
    16201 ggtaatagtg gtcaggaatt aaatgaaaaa gatgttaggg ttgttagcta agtataacat
    16261 cataacgctt acaaaacaat gcaagaagga taaattatta tgagaactta ctggtatgtg
    16321 tcattaaaca ataaatatcc gaagccaatg aaaggacagc ataggcgtgt agtcatgtct
    16381 gttcaaatga aggcgaagta ttcgattgta gaaatgctaa gagaagctac accaaaagag
    16441 attgatcagt ataagttggt tcttattggc attggttggt ttaaggatga acatattcaa
    16501 acaaacatga agaggtggtt gagatgaagt tatattatgt tgaaaaagca aattgttggg
    16561 tggttgaagg ctgtctgtca aagtacgcta cagatattat atctactcaa ttttcagagt
    16621 tgggattgaa ggttatcttt tgtggtgagt ttgtcagtgt gaaagaagcg gtattgccaa
    16681 tgacagcaaa gagcagacgc tcgcgtggtt caagtt
//
LOCUS       DADVXO010000061        16324 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.063, whole genome shotgun sequence.
ACCESSION   DADVXO010000061 DADVXO010000000
VERSION     DADVXO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.063"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(95..1030)
                     /gene="murB"
                     /locus_tag="KB950_001656"
     CDS             complement(95..1030)
                     /gene="murB"
                     /locus_tag="KB950_001656"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBB6857877.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1103..2023)
                     /locus_tag="KB950_001657"
     CDS             complement(1103..2023)
                     /locus_tag="KB950_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6857878.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2136..2888
                     /gene="xth"
                     /locus_tag="KB950_001658"
     CDS             2136..2888
                     /gene="xth"
                     /locus_tag="KB950_001658"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBB6857879.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3752..4285
                     /locus_tag="KB950_001659"
     CDS             3752..4285
                     /locus_tag="KB950_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBB6857880.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4775..5299)
                     /locus_tag="KB950_001660"
     CDS             complement(4775..5299)
                     /locus_tag="KB950_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857881.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5292..5765)
                     /gene="tsaE"
                     /locus_tag="KB950_001661"
     CDS             complement(5292..5765)
                     /gene="tsaE"
                     /locus_tag="KB950_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBB6857882.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5888..6868)
                     /gene="pta"
                     /locus_tag="KB950_001662"
     CDS             complement(5888..6868)
                     /gene="pta"
                     /locus_tag="KB950_001662"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBB6857883.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7021..7701)
                     /locus_tag="KB950_001663"
     CDS             complement(7021..7701)
                     /locus_tag="KB950_001663"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBB6857884.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7827..8696
                     /locus_tag="KB950_001664"
     CDS             7827..8696
                     /locus_tag="KB950_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6857885.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8722..9237
                     /locus_tag="KB950_001665"
     CDS             8722..9237
                     /locus_tag="KB950_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857886.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9284..9877)
                     /locus_tag="KB950_001666"
     CDS             complement(9284..9877)
                     /locus_tag="KB950_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857887.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9902..10081)
                     /locus_tag="KB950_001667"
     CDS             complement(9902..10081)
                     /locus_tag="KB950_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857888.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10158..10667)
                     /locus_tag="KB950_001668"
     CDS             complement(10158..10667)
                     /locus_tag="KB950_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857889.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10763..11143)
                     /locus_tag="KB950_001669"
     CDS             complement(10763..11143)
                     /locus_tag="KB950_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6857890.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11473..12222
                     /locus_tag="KB950_001670"
     CDS             11473..12222
                     /locus_tag="KB950_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBB6857891.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12406..13683)
                     /locus_tag="KB950_001671"
     CDS             complement(12406..13683)
                     /locus_tag="KB950_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857892.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13762..14406)
                     /gene="pcp"
                     /locus_tag="KB950_001672"
     CDS             complement(13762..14406)
                     /gene="pcp"
                     /locus_tag="KB950_001672"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBB6857893.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14605..15549)
                     /locus_tag="KB950_001673"
     CDS             complement(14605..15549)
                     /locus_tag="KB950_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBB6857894.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15551..16237)
                     /locus_tag="KB950_001674"
     CDS             complement(15551..16237)
                     /locus_tag="KB950_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBB6857895.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4707 a         3386 c         2883 g         5348 t
ORIGIN      
        1 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
       61 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
      121 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      181 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      241 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      301 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      361 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      421 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      481 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      541 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      601 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      661 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
      721 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
      781 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
      841 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
      901 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
      961 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     1021 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1081 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1141 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1201 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1261 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct
     1321 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1381 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1441 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1501 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1561 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1621 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1681 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     1741 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     1801 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     1861 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     1921 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     1981 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     2041 catttagtta ctttcatctt acaccatttt tttctaaaat gaggtacaat agtatcgcaa
     2101 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2161 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2221 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2281 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2341 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2401 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2461 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2521 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2581 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2641 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     2701 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     2761 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     2821 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     2881 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     2941 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     3001 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3061 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     3121 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     3181 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     3241 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     3301 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     3361 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     3421 caaatggcaa aatagttcct catattttgc tattttttga gctaataccg agggtcagct
     3481 gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa
     3541 atttttttga aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca
     3601 tgatccccat ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa
     3661 atattacata aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt
     3721 tacagtaatt tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac
     3781 tgccaatcat cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca
     3841 gatcgttgat gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt
     3901 ccaaattcac ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg
     3961 gcaaactatc caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt
     4021 tgatacaaag gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct
     4081 ttctttatgt acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt
     4141 gaacactgtg tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga
     4201 tagtcgtgca tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt
     4261 gaaaaaactt gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag
     4321 cctgccgttt cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt
     4381 aaatattgtg actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt
     4441 gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     4501 ttcatttggt tggacttatt accgcagatg caagcttttg gacaccgttt accaagaggt
     4561 tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggcaaaata gttcctcata
     4621 ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt tattcaaaaa
     4681 aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg ccttctcaaa
     4741 aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa ctcattttat
     4801 atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc gcttcgattt
     4861 ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga acgtcaagct
     4921 ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct agcataagag
     4981 tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct cccacgtgag
     5041 acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc ccttcataga
     5101 ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct tcttctaagg
     5161 tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc tccttattaa
     5221 cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc cgcaagatga
     5281 tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg tttgaaggtt
     5341 tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc tgtatcactt
     5401 ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag atttccccat
     5461 tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc tgctccgcta
     5521 gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact atattctcta
     5581 ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg ggcaattcct
     5641 ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt gtcacctggc
     5701 attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc tgatagttcg
     5761 atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg taatgaagtt
     5821 tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc tagttcgcgt
     5881 gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta tatacatctt
     5941 cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt agaattggtc
     6001 cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt cctgactgca
     6061 attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc gctttcagtt
     6121 gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc atgtattgtg
     6181 gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct ggcgatttcg
     6241 ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg aacaattctg
     6301 ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga ttcacgttga
     6361 tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg atcatcgctc
     6421 cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg cgaacagtgt
     6481 cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata tacgtaagca
     6541 tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg gatgcctttc
     6601 catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa ttatcagggc
     6661 tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt cttatttctt
     6721 cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg gcagctgcgc
     6781 ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg cgaacaattt
     6841 taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt atatgtatta
     6901 tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc aaaaatgctg
     6961 gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag cgctgtttta
     7021 ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa ggagcgcatc
     7081 attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata acggactggg
     7141 atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt tttcttgtgc
     7201 cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt tttcaatgat
     7261 cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc cttcccgaac
     7321 ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta gatttccatg
     7381 ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc ttaacgatgg
     7441 cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat tttctccatg
     7501 ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca attccaatgc
     7561 ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt tttttaaaaa
     7621 ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt cttgccaact
     7681 gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac tcacattttc
     7741 ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa actaggaaca
     7801 agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat atggacggca
     7861 cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata cgttttgcaa
     7921 atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa gctcgcgcag
     7981 ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca caggtatttg
     8041 ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca atctctgttt
     8101 tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat ggtctttatt
     8161 ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc catttacctc
     8221 accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta ttgcacatca
     8281 catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt ttaaaaatta
     8341 tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag atttctcttt
     8401 taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac cataaaaacg
     8461 ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct ttagaagaag
     8521 tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca ggagtcagtt
     8581 tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca acggacacaa
     8641 accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac ttgtagtttt
     8701 atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca gcttggacgt
     8761 gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat ggttggtcgc
     8821 tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct cgttgctcac
     8881 atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga gttcgaaaag
     8941 gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat tcgtagactt
     9001 cactggacag tccaaggtga tatctataat catcaatatc aaatccatca tttcaatcag
     9061 caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt attggatgtc
     9121 tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga ttactggctc
     9181 taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt aagttaaaaa
     9241 gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata ttcttttgca
     9301 tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag tcatggcgtt
     9361 cgtataattt tttagccttt ggattttttt catccacact tagaccgatg acggagcgat
     9421 tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg ccttgtcctc
     9481 taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac gtttctttat
     9541 caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt accagtggct
     9601 gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca actttaccgt
     9661 ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca gtgattgctt
     9721 cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc agttccatat
     9781 cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt ctcgcaaaac
     9841 gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa tcgcaaaatc
     9901 gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta acgattcttg
     9961 ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta tgttcgacta
    10021 cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc attttttcca
    10081 tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt gtaaaaaaga
    10141 gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc tccatctatt
    10201 aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc tttcactttt
    10261 gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg tttgaactct
    10321 tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt tcgtacggtt
    10381 gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa ctggtattca
    10441 tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat agcagatatg
    10501 ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga gatccgatat
    10561 tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt cataccggta
    10621 atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct tcattctcct
    10681 gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat gaaacgaaaa
    10741 aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg tctggatcaa
    10801 agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct gattcgatcc
    10861 ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa gcaagatgac
    10921 gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt ccaaaaatct
    10981 ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct ttgcggtaat
    11041 tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat ttctgataat
    11101 ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca ctcctattct
    11161 tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc agattgaaaa
    11221 aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag taggtcctcc
    11281 aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat tttcaagaaa
    11341 acaaacagaa aattatcata acacaaatta tttataattg aaagactagt tttttttcaa
    11401 gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta gtgtcttaag
    11461 gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa aggctggccg
    11521 caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac tcttgtcaat
    11581 agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg tgtattgtgt
    11641 attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt gtctgcgatt
    11701 tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga acagattgct
    11761 tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca taatatggct
    11821 tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag tactttcctt
    11881 gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg gccgtgggta
    11941 gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt gcgttacaac
    12001 aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg gtatatttca
    12061 tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta cttgctgaat
    12121 gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga acgtatgcaa
    12181 aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg agcgtgggac
    12241 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aatcagaagc
    12301 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12361 ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata gtaattcctt
    12421 cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct gataatattc
    12481 caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt tgtcgattgt
    12541 ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt cttttgagac
    12601 tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca atacttttaa
    12661 aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa aaccatagcg
    12721 acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt ggtttttcgc
    12781 aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct ttctcaacac
    12841 ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat atacgatgga
    12901 ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta catcctttac
    12961 taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt ttgactgccc
    13021 attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat ccgctatgta
    13081 aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta tatccggtaa
    13141 atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt gccatagtcc
    13201 ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg ccagatcaac
    13261 ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca ggtgatcggc
    13321 aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa cgatgtattg
    13381 cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt ttgttcgaaa
    13441 aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat aatgttcttc
    13501 cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc gctgtttttt
    13561 cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag aaattttgat
    13621 gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt gccattcttc
    13681 cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa cgttagcaac
    13741 cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa tatcttcttt
    13801 attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt ctaaagacat
    13861 agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca cgtggatgaa
    13921 cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat acatgatatc
    13981 attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat gatcttgtac
    14041 attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac cgttttcttc
    14101 taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt ctgctagatt
    14161 gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga cacagatcac
    14221 gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt tttcgaatac
    14281 ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca ttttttttac
    14341 tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg ttactagtac
    14401 tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt atcataccat
    14461 tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca gatcaaactt
    14521 aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt gcgacaatta
    14581 ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa tttggaaaac
    14641 tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt ctttcatctc
    14701 cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca gggtcccaca
    14761 aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat gactaaatac
    14821 gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat tccccatgat
    14881 taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca catttccttc
    14941 tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc ttgtaaatac
    15001 cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg tgcccagcat
    15061 atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga acatcaagat
    15121 tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat tggtaaatag
    15181 agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt tcattcctag
    15241 tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc ctttgctgac
    15301 tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca cgattgccgg
    15361 aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg ctgacccata
    15421 acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca atacgagtcc
    15481 catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag gatttgtaaa
    15541 aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc ttttctttaa
    15601 gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact tccactaaag
    15661 caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg actagcaacg
    15721 cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt tccatagctg
    15781 aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct tttgattcga
    15841 tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact ccaccaaaac
    15901 tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca atcataccag
    15961 cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga ccatttcttt
    16021 caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga ttgtttacaa
    16081 aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc aaagctgtaa
    16141 caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac ccgagaataa
    16201 caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca taaaatgaca
    16261 atattttttt aattataaag caaaacacat aaaaaatata gttgttttct cacgaaatag
    16321 acac
//
LOCUS       DADVXO010000062        15936 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.064, whole genome shotgun sequence.
ACCESSION   DADVXO010000062 DADVXO010000000
VERSION     DADVXO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.064"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            226..894
                     /locus_tag="KB950_001675"
     CDS             226..894
                     /locus_tag="KB950_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBB6857896.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="KB950_001676"
     CDS             complement(1094..1324)
                     /locus_tag="KB950_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857897.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="KB950_001677"
     CDS             complement(1451..2833)
                     /locus_tag="KB950_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6857898.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="KB950_001678"
     CDS             complement(2989..4176)
                     /locus_tag="KB950_001678"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBB6857899.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="KB950_001679"
     CDS             complement(4183..5214)
                     /locus_tag="KB950_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6857900.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="KB950_001680"
     CDS             complement(5260..6684)
                     /locus_tag="KB950_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6857901.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="KB950_001681"
     CDS             6859..7764
                     /locus_tag="KB950_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB6857902.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="KB950_001682"
     CDS             7855..8532
                     /locus_tag="KB950_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBB6857903.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="KB950_001683"
     CDS             8529..8699
                     /locus_tag="KB950_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857904.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="KB950_001684"
     CDS             8813..9709
                     /locus_tag="KB950_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6857905.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="KB950_001685"
     CDS             complement(9764..11134)
                     /locus_tag="KB950_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6857906.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="KB950_001686"
     CDS             11372..12118
                     /locus_tag="KB950_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6857907.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="KB950_001687"
     CDS             12115..12954
                     /locus_tag="KB950_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6857908.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="KB950_001688"
     CDS             12955..13905
                     /locus_tag="KB950_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBB6857909.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="KB950_001689"
     CDS             14046..14798
                     /locus_tag="KB950_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857910.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15936
                     /gene="queG"
                     /locus_tag="KB950_001690"
     CDS             14817..>15936
                     /gene="queG"
                     /locus_tag="KB950_001690"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBB6857911.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEH"
BASE COUNT         5165 a         2990 c         3214 g         4567 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatg
//
LOCUS       DADVXO010000063        15900 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.065, whole genome shotgun sequence.
ACCESSION   DADVXO010000063 DADVXO010000000
VERSION     DADVXO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.065"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(52..522)
                     /locus_tag="KB950_001691"
     CDS             complement(52..522)
                     /locus_tag="KB950_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBB6857912.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="KB950_001692"
     CDS             complement(681..1139)
                     /locus_tag="KB950_001692"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBB6857913.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="KB950_001693"
     CDS             complement(1310..2455)
                     /locus_tag="KB950_001693"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBB6857914.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="KB950_001694"
     CDS             complement(2468..3385)
                     /locus_tag="KB950_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBB6857915.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="KB950_001695"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="KB950_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBB6857916.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="KB950_001696"
     CDS             4477..4647
                     /locus_tag="KB950_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857917.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="KB950_001697"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="KB950_001697"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBB6857918.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="KB950_001698"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="KB950_001698"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBB6857919.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="KB950_001699"
     CDS             6911..7792
                     /locus_tag="KB950_001699"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBB6857920.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="KB950_001700"
     CDS             7789..8505
                     /locus_tag="KB950_001700"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBB6857921.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="KB950_001701"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="KB950_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBB6857922.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="KB950_001702"
     CDS             9222..10223
                     /locus_tag="KB950_001702"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBB6857923.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="KB950_001703"
     CDS             10220..11581
                     /locus_tag="KB950_001703"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBB6857924.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="KB950_001704"
     CDS             11541..12692
                     /locus_tag="KB950_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBB6857925.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="KB950_001705"
     CDS             12667..13836
                     /locus_tag="KB950_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6857926.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="KB950_001706"
     CDS             14011..15390
                     /locus_tag="KB950_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6857927.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="KB950_001707"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="KB950_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2803 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DADVXO010000064        15757 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.066, whole genome shotgun sequence.
ACCESSION   DADVXO010000064 DADVXO010000000
VERSION     DADVXO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.066"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            90..1517
                     /locus_tag="KB950_001708"
     CDS             90..1517
                     /locus_tag="KB950_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6857928.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1609..1974)
                     /locus_tag="KB950_001709"
     CDS             complement(1609..1974)
                     /locus_tag="KB950_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBB6857929.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1971..2336)
                     /locus_tag="KB950_001710"
     CDS             complement(1971..2336)
                     /locus_tag="KB950_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBB6857930.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2528..2776)
                     /locus_tag="KB950_001711"
     CDS             complement(2528..2776)
                     /locus_tag="KB950_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857931.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2775..2903
                     /locus_tag="KB950_001712"
     CDS             2775..2903
                     /locus_tag="KB950_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBB6857932.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2960..4654
                     /locus_tag="KB950_001713"
     CDS             2960..4654
                     /locus_tag="KB950_001713"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBB6857933.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4658..4777
                     /locus_tag="KB950_001714"
                     /pseudo
     CDS             4658..4777
                     /locus_tag="KB950_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4754..4927
                     /locus_tag="KB950_001715"
     CDS             4754..4927
                     /locus_tag="KB950_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857934.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5305..6438
                     /locus_tag="KB950_001716"
     CDS             5305..6438
                     /locus_tag="KB950_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBB6857935.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6469..6789
                     /locus_tag="KB950_001717"
     CDS             6469..6789
                     /locus_tag="KB950_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBB6857936.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6818..7282)
                     /locus_tag="KB950_001718"
     CDS             complement(6818..7282)
                     /locus_tag="KB950_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857937.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7414..8667)
                     /locus_tag="KB950_001719"
     CDS             complement(7414..8667)
                     /locus_tag="KB950_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6857938.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8696..9013)
                     /locus_tag="KB950_001720"
     CDS             complement(8696..9013)
                     /locus_tag="KB950_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6857939.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9039..9359)
                     /locus_tag="KB950_001721"
     CDS             complement(9039..9359)
                     /locus_tag="KB950_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6857940.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9823..11238)
                     /locus_tag="KB950_001722"
     CDS             complement(9823..11238)
                     /locus_tag="KB950_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6857941.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11255..12571)
                     /locus_tag="KB950_001723"
     CDS             complement(11255..12571)
                     /locus_tag="KB950_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6857942.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12721..13419)
                     /locus_tag="KB950_001724"
     CDS             complement(12721..13419)
                     /locus_tag="KB950_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBB6857943.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13708..14664)
                     /locus_tag="KB950_001725"
     CDS             complement(13708..14664)
                     /locus_tag="KB950_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6857944.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14777..15610)
                     /gene="msrA"
                     /locus_tag="KB950_001726"
     CDS             complement(14777..15610)
                     /gene="msrA"
                     /locus_tag="KB950_001726"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6857945.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4902 a         3194 c         2695 g         4966 t
ORIGIN      
        1 ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt
       61 gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt
      121 taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc
      181 tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa
      241 aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt
      301 tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt
      361 taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat
      421 gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta
      481 atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc
      541 tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat
      601 cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt
      661 atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc
      721 aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt
      781 acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag
      841 aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt
      901 tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa
      961 aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt
     1021 tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc
     1081 cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa
     1141 ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc
     1201 gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct
     1261 atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa
     1321 ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt
     1381 atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg
     1441 tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag
     1501 ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa
     1561 gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg
     1621 gacatctacg ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac
     1681 caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc
     1741 agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc
     1801 attgttctcg ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa
     1861 atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc
     1921 cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc
     1981 tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact
     2041 gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact
     2101 gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc
     2161 agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac
     2221 caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa
     2281 atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac
     2341 gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt
     2401 cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc
     2461 ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa
     2521 ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac
     2581 ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat
     2641 ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc
     2701 gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca
     2761 aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg
     2821 cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat
     2881 atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga
     2941 agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc
     3001 caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac
     3061 cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga
     3121 tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg
     3181 tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc
     3241 tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg
     3301 aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact
     3361 gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga
     3421 agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt
     3481 tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc
     3541 tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct
     3601 agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga
     3661 gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt
     3721 caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact
     3781 gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg
     3841 gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga
     3901 ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa
     3961 tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac
     4021 gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc
     4081 cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat
     4141 gtctattgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat
     4201 ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa
     4261 aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg
     4321 accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc
     4381 tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa
     4441 agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta
     4501 tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt
     4561 tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac
     4621 tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct
     4681 gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga
     4741 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaagctgc
     4801 cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt
     4861 agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagg
     4921 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
     4981 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
     5041 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
     5101 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
     5161 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
     5221 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
     5281 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
     5341 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
     5401 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
     5461 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
     5521 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
     5581 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
     5641 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
     5701 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
     5761 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
     5821 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
     5881 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     5941 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     6001 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     6061 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     6121 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     6181 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     6241 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     6301 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     6361 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     6421 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     6481 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     6541 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     6601 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     6661 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     6721 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     6781 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     6841 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     6901 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     6961 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     7021 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     7081 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     7141 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     7201 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     7261 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     7321 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     7381 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     7441 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     7501 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     7561 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     7621 gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt
     7681 caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat
     7741 cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa
     7801 cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt
     7861 gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc
     7921 tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa
     7981 ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc
     8041 cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc
     8101 agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga
     8161 agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc
     8221 atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa
     8281 caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat
     8341 cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa
     8401 agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt
     8461 ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt
     8521 attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc
     8581 ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt
     8641 ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg
     8701 cgttcaatcg acgatacaga tcaatgatct cttttgctaa atctgtaaaa gctatagatg
     8761 tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg
     8821 cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat
     8881 ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca
     8941 ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt
     9001 tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat tagtttttct
     9061 gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt gattacatct
     9121 aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc
     9181 ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca
     9241 gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg
     9301 actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt
     9361 ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa
     9421 aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact
     9481 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
     9541 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca
     9601 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac
     9661 tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt
     9721 tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca
     9781 aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc
     9841 aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa
     9901 tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc
     9961 attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc
    10021 ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc
    10081 atcttgcacg attcctttct catccatata ctgttcttca tattctcttc ccataccgtt
    10141 ttctgttatc agccaaggga tattatgata attttctttt agatacatgg ctatatcata
    10201 catgattttc ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc
    10261 aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg
    10321 ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg
    10381 aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc
    10441 caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa
    10501 caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat
    10561 ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac
    10621 tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg
    10681 ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt
    10741 gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt
    10801 agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc
    10861 tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc
    10921 gatcatttca cttatatgct gagcatattc ttcatctact atacagttct cataatcagt
    10981 aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag
    11041 atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc catacccatt
    11101 atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt
    11161 ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt
    11221 tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat
    11281 ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg
    11341 taataactgc accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg
    11401 gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa
    11461 tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga
    11521 tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca agggataact
    11581 tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga
    11641 tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata gtaataatgt
    11701 tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca
    11761 acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt
    11821 gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc
    11881 gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa
    11941 atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag
    12001 cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca
    12061 accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg
    12121 tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta
    12181 attcataaac agttggatta acagataaat aagacatcac tgcgatgata ccagggaata
    12241 caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg
    12301 tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa
    12361 aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca
    12421 atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca
    12481 ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt
    12541 tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct
    12601 ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt
    12661 accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg
    12721 tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg
    12781 gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc
    12841 tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa
    12901 agcaattgat tcattaatga ttgggataat atgcagcaag gctacgtttc cgctagcttc
    12961 tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa
    13021 agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata
    13081 ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg
    13141 tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg
    13201 cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag
    13261 agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata
    13321 ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt
    13381 gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc
    13441 tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtatac
    13501 aaaattgatt aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac
    13561 taggataaaa ttcactccta gccttcgatc atttttattt tgaacgacaa aaaatatgat
    13621 gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt ttttaatata
    13681 tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc tcaattcttc
    13741 ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact gacttaaccg
    13801 acttgctccg accagtacac ttgtaacaga tttttgtcgc aaattccatg caagggccat
    13861 ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga ttttttccag
    13921 agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc
    13981 ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg gactgaacgt
    14041 gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta gtagtgcacg
    14101 agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc tttttaaaat
    14161 ttcagtcatt ttttttgtat cttctccatt atagttggat ataccaatat ataatgcttt
    14221 tccttgtctt acaagttgca tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc
    14281 tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga gactttggtc
    14341 acaacttgaa atgatggatt tacgagagcc ccattcccca tatggccctt cccacatatg
    14401 ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat cttctctaaa
    14461 aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat tcgcaaggtc
    14521 gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat tatggatagg
    14581 atcataatca ccaaaatttt gccataatcc gagagataaa atcggtaatt tcaaacctga
    14641 atgaccggat ttacgatatt ccattttttc ataacgtgca ctatctgcaa tatacaattt
    14701 ttctcctcct catcaaatct ttttaaaacg ctttcttttt tctattttta gtttactaca
    14761 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
    14821 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta taacgtttcg
    14881 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    14941 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    15001 gttccgctaa ttctttttgt cgttcgactt gataaaaaat cactggacga tattgcttcc
    15061 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    15121 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    15181 ctgtgtaacc accacttact tgatcataat ttggtttttc aacatgtccg cccgtgtatc
    15241 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    15301 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    15361 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    15421 caggcgtcag attcattcgg atagctaatg gacgtagtgt tgcttctagt tttgataact
    15481 gttctttaac ggatatattt tctcttatgt cattctttgt ctgaatcaat acttttcgtt
    15541 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    15601 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    15661 tatcataaag tgaggaaata agcgattact tcatccccct acagattttt cgtataaaaa
    15721 agagagaatc cttatcgaca gattctcctt tttaatc
//
LOCUS       DADVXO010000065        15604 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.067, whole genome shotgun sequence.
ACCESSION   DADVXO010000065 DADVXO010000000
VERSION     DADVXO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.067"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..173)
                     /locus_tag="KB950_001727"
     CDS             complement(<1..173)
                     /locus_tag="KB950_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6857946.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="KB950_001728"
     CDS             complement(593..1714)
                     /locus_tag="KB950_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBB6857947.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="KB950_001729"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="KB950_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBB6857948.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="KB950_001730"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="KB950_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBB6857949.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="KB950_001731"
     CDS             complement(4304..5215)
                     /locus_tag="KB950_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6857950.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="KB950_001732"
     CDS             complement(5232..6239)
                     /locus_tag="KB950_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB6857951.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="KB950_001733"
     CDS             complement(6236..8365)
                     /locus_tag="KB950_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6857952.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8513)
                     /locus_tag="KB950_001734"
                     /pseudo
     CDS             complement(8370..8513)
                     /locus_tag="KB950_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(8666..9853)
                     /locus_tag="KB950_001735"
     CDS             complement(8666..9853)
                     /locus_tag="KB950_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBB6857953.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(9946..12291)
                     /locus_tag="KB950_001736"
     CDS             complement(9946..12291)
                     /locus_tag="KB950_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6857954.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            complement(12278..12667)
                     /locus_tag="KB950_001737"
     CDS             complement(12278..12667)
                     /locus_tag="KB950_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6857955.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            complement(12717..13613)
                     /locus_tag="KB950_001738"
     CDS             complement(12717..13613)
                     /locus_tag="KB950_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6857956.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            complement(13616..15463)
                     /locus_tag="KB950_001739"
     CDS             complement(13616..15463)
                     /locus_tag="KB950_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBB6857957.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
BASE COUNT         4705 a         3224 c         2583 g         5092 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttttcagtca agtccacaat cttgtgtaaa
     8521 agttcaacct catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc atgcctttct
     8581 ttctgaggag agaggtcagg agcagtagcg cagcgactct tgatgtctct cctcagaaag
     8641 aagagactcg cacaggttca tggtgctaaa tgttattcga attaaattct aaatattttc
     8701 gtggagaagc cagccactca gtgtcttttt ccatcaaaag tgttccaatt atccgagtga
     8761 tggaattgat atttggaaaa atccgaatca ctcgttctct ccggcgaatc tcttcgttca
     8821 accgttccaa cacattggtt gtacgaatcc ttcggcggat attttcagga aaagacaaaa
     8881 tttgaatagc atcttcaaag ccgttttcta aaatgtcgca agcagcactt aattttgaat
     8941 cacaaccata tttttccaag aagtgctctt ttctttcacg tgtcaattca agttcagagg
     9001 ctttaaagat actcttcaac tcgtctttta catcagaaga aactttcttt ggcagcttat
     9061 caaaaatatt tctcaaaaag tgggcttggc atctttgcca gctcgcacct aaaaagttct
     9121 tgcgaacagc cttaactaac cctttgtgtg catcagaaat gaccagtttt acaccgaaca
     9181 aacctcgttg aacgaacgat tgaaaaacgg tatcccaatt atcttccgat tcgccgtctt
     9241 gaacatcaaa accaagaatc gtacgtcgtc catcagaatc aatgccgata ccaatatata
     9301 caccttttga gacaatacga tgattttccc ttactttcat atacagggca tcacacatga
     9361 aaaatggata ttccgagcct tctaggctcc ggtttctcca ctcaaaaaca aatggatcca
     9421 actgtttcat cagatttgaa acgaatgatt tagaaacagt cgcaccatcc gttagcagtt
     9481 caactgtttt agttactttg cgagtggaga caccagaaat gaccatttca atcatggcag
     9541 cgatcaaagc tttttcgctt cgctgatatc tttcaaagat agtaggacta aacttaccgt
     9601 ctctggttcg tggaacatga agttctaacg taccaattcg agttgtaaac gaacgatcgt
     9661 agtaaccatt tctttgtgat tgccgttctt ctgaacgttc ataggcattt gcctgaatat
     9721 attgatttcg ttgttcttcc atcagctgat taaacatgat agtcataagt gtttttgcgg
     9781 tatcattcgc accgctttcg tttataattg cttgaatttc ttcgctttca aaagtaaaat
     9841 gtacttgagt cattgatatc agtcctattc gagtatgttt tctgcaaaaa acattgtact
     9901 cctaactgag atcaatggct tttttgatgc ttcactttta cacaattata tggacgtaat
     9961 cccgttttcc aaatctcgca ggcgtttttg attgctttga tcctcctgat ccaaaccaaa
    10021 gaattccaag gttttcttga tttcatgaat atcggtactg cgaaaagcaa acttcaaccc
    10081 gatattattt tttaaacgtt catctaataa atcgtcgcta ttttgagtga cgaaatagac
    10141 cccagcattc atggcccgac cagcacgaac aagcttattc gataacgttt ttccttgagc
    10201 cacttgaaga aaactccaag cctcatccaa atcgacaatt ttgaagattt ctcggttact
    10261 atggataaag tccaaggcaa aagtggaaat gacgattagc atcgaaacac taagcaattc
    10321 catggtggta tattcttcaa aagtcgtttc tgcatccggt aaaaccaaat ccgccacttg
    10381 aatgatattt agctgtttat ctaaactaat cgattgggta atcgagccgt ctgagaaaag
    10441 caaatgagca aaatcataat cggtaaagga ctcgatatga tcggcgatat tttccgcgat
    10501 tggtgacccg tcttttcgca attcgttaat aacttgtaat aacccccgtt gatcactttg
    10561 agtcaccgtt cgaatggctt tacgtaacac aggaaacttc tccccgtctc gacttgaaat
    10621 tcctgttaaa aaagtcagaa tatcaattgc taaactttcc gagtctttct ttttcttcat
    10681 aatcacataa ggatcaagca gaccttgatt ttctgactta ctcgtcaagt tgaggatact
    10741 gatttcatcc gcaatttctg gcaaggtttc tttccaccct cctcgctccc cttttggatc
    10801 gacaatcaca gcttgaccac cgaacaaaac agcatagtag actaagagat tgttactaaa
    10861 ggatttacct ccaccaagag aacccaggaa agcggcggcc agcgcattgg ttactgaacc
    10921 cttgactcct tgtgaagcca atgccggttt caggtaaaca ttacgacctg tatccacatt
    10981 gtagccaaag taaatccctt ctaattctcc tagcatctga gtagccccaa aaccaagccc
    11041 cgccaaaaaa tcagacgtaa catactgaat atagtcgttc atatatcgtt tacttgaggg
    11101 taagaattct tcatgtagcc ccatcatatc gccaaagggc cggactagtt ttacattggt
    11161 atcatcataa aaatcgagca cttcatcaca gcgtcgcttt agttcatcca ctgattcggc
    11221 actcactcga accacataac tcagtttgta catagattct tttgactgat ccaaggtggt
    11281 ttccaattca tccacggaat ccaaagcatc taagacattg gaattcgttt cattgtcaga
    11341 ttggtaagca tgattgtcta aatctttcag ttcttttttc ttattacgaa cggtagctaa
    11401 agccttttta ttcgtcacaa tctctacatt catacttgtg tcgattggga atgtaaattg
    11461 ttgttgctgg taatagaaaa gttctgagga aggaaattcc atctccccca caattgtatt
    11521 aattgttaaa tacgctacgt acgactcatg attctcatgt tccatccgta agcaccttgg
    11581 tttttcttca attaaacaac gatttggacg taataagtca taacgtttca ccagtgtttc
    11641 tgattttagc tttttctttg gtagctgaaa ttcgtattct tcaaacggta tccctttttc
    11701 gccataaata tgttcaatca aatacgtcag gtcactcggt gtgactcgcc tgactttaaa
    11761 tcttcgagcc aatttgcttt ccattaattt ttcaagctta gaatagcagc gaatttcttc
    11821 attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga caaattcttg
    11881 aagcccagaa aagaaagatt tcttgagact ctttagattg acttcttcat ctgtcgcaat
    11941 tagtttaaag ccaataaaaa agcgataatc aatctgacta tcgccaatca tggatactaa
    12001 tgcatctgtc tgtatatcaa ttcgttgttt cgctacttct ttgagtctac cggtaatttc
    12061 tttttttgat cgttcttgtg tggcgcgaat actactttct gtggcaattt gtaacgcatg
    12121 gatcttgcct tcacgatttt gcgcaattag ttggcgaaag ttatcatgca cttgaaattt
    12181 ctgttcgggt gacaaaaagg agtaattgta aggcactaat tcatagtagg caaaacactc
    12241 gccttcttgg ttaaagacga gattattttc aatatactta atcggataca tagttcacac
    12301 tccttacaag agtactgggt tcatcccatc gtttcttctg ttctttgact ttctttcctg
    12361 caaaagtcac cttaaatcgc aagtgatagc tgataaagga tcgtaaaaat cccagtggtt
    12421 tcttgccatc aaacgtcttt tgcgacataa accaagtgac agccaccggt atgcctaagt
    12481 attttaaaaa gacctcctct atcatagata gtggaggcac atgggcaaat acgatgacaa
    12541 acagtaaaga taaaacgaac caagccattt ggttaaaagt cacaggaaaa ggcaactgaa
    12601 aatcattgat tgcgtaaatg actttttcaa ctgcccagat actggtatag ctttttattt
    12661 ttttcatttt tcctcctaaa aagggcggac ataataatat gtctcaccct ctagtgttat
    12721 tctacaattt gtttaaaacc atagcgtttc gctctatttg taaaagcatc ctcgccacct
    12781 tccatcacag tacatattct tggcttgata ttgggttcat ctaccgaccc gcactgatca
    12841 gccgatattt cttttgaatc tcccatgcta atcaaccatt ctgaatcacc attaactggg
    12901 atatttgcat tatgagttac aattaaaatt tgtctgtttt ccttttttgt aatgatactt
    12961 cttacgatta agttattaat taattgacta tccaaatcat cttcaggctg atccaatatg
    13021 agtggtgaat ttccatacgc caaaatcata gaaagaattg cagatgtctt ttgaccagca
    13081 ctagcattcg ataaactaac tttttttcca tttagttcaa gtcggataac tactaaatca
    13141 tttggcttaa gggtttgtaa ttcaagaact tgtgcatcat tcaattttct tatagaattg
    13201 attaattttt tatctgaaaa aatactactt tccccactaa ctcttatatc aagtatgtca
    13261 ttatatatat cttgattagt aatggaattt tttgtcagct tatcaataag agatccaaac
    13321 tcactgtcaa aagtgtcctc cttattaata caagctctga gctctttaac ataattattg
    13381 aaatccctat ttggttgaat agtagcctct attcctgtaa tatcagaaaa aatttcgcgc
    13441 gaggaactac ttctctcaga atagagattt tgatatgatt tattcattct tgacattgct
    13501 gagtcaacct caacttggta atctttaagc tttatttctt gttgaataat ttcttccaat
    13561 cgctcttcag tttttgataa agattgatat aaagaatcaa ggttttgtaa catttttaat
    13621 tcatcttgag ataactcttc actaagtttt tgatacctct ttgatacctc tttgaaacct
    13681 cattatagtt aactttccaa tctgatttat tcagctctgc tctataacta tcaatttcat
    13741 ctgacatttc attaattatt gtttgaatct tagcccgatt ggtcactaaa ttcttaatca
    13801 attctccgcg gtgttcatca atttcgtctg taacttttat accattgcca aagtcaattt
    13861 tgtcaacact atcagttaga tttttgggaa tttccttaag tgcctccaca tcacgtttca
    13921 gttccctata aatatctgtc tcttttgaat actcatcata aacatcttta taactcttat
    13981 gagatagctt ttctacttgt ttttttaaat cttcaatttc aagctctacc ttcgccttat
    14041 cttgtatttc ttttttcaca gaaacaatat cccaatttaa ttttcttatt tcgtttacac
    14101 atgtttcaat ctcattttta tactctgaaa tcaaatcaga gttatatcca tctagtaatt
    14161 ctagtatact agtttgattt tttgaaattt cataaatttg tttttgagag tatatactca
    14221 ccttatcgga aatcattctc aaccgttctg tttctttttc aaccagacca ttctcttgtg
    14281 taaattcata aatagaatgt ctatcctgtg ttcttattat ttgatatttt gtccccttat
    14341 agaaaatatt cagtttcaca attgtatcct tcaaaaatat tccagagcca ttatgttcaa
    14401 tttgtgcaaa gttctgataa tcactttgta tttcagaaaa ttgatcaagt tctgcttcct
    14461 ttccaaggac atagaccaag aatcttgtaa tacaagattt gcctgttcct cttcctccaa
    14521 taattgaagt taaattatcg ctgaattcga ctacaacttc ttttgtgcta agagtggtcc
    14581 cagaaaagct taatttttcc aataaaactt cattttcatt aatggagaaa ttagaaaaat
    14641 cagaatgaac tcgagtttcg cctagcatta aagcatcgcg aagtgatccc aagtcaggtt
    14701 cgtccttcat ttttatatag gaatatcttg tgcctattcc ccaaagtcca tgttttgaat
    14761 ttcctttcga gtgtgggtta tcagaaaagc taagaaatgt catattattt ttttctgcct
    14821 caacaactga tttataccaa gattccaaag tatcttttcc aactctacca tcatatctgg
    14881 cattcacact atcatagata gtgtcacgct tagatgaggg aatcatttgt gagcgatttt
    14941 caaaaaattc tttttgaacc gtctgtactg catatatatt ttctgattca aatatctttc
    15001 ctagatttcc aaattttgct gaagaaattc cattaaactc atctatatgt gcaggaataa
    15061 cgagcaagcc ctcgtgctca gcaaaatcaa taacttctaa aactgatttc attgagcaag
    15121 ctgcagcgct ggcccaatcc tctcttttta tacccatctt ggttaagaaa tcagagatat
    15181 catttgtaca tgcactttta tcaaatagga ctagtaaatg agttccattt tctccacacg
    15241 ttaattccac tccaggaaaa aaatctaagc cattttcgat agccactttc ttatattcat
    15301 caatattttc ccctgtattg tgatcagtta atgcaacaca atcgagtcct ttgtttaaac
    15361 attcttctac ccattcctta gcagtaacct ttttatcctg aaagcactga ctcgcagttg
    15421 aatgaatatg aagatcacat ttgtaccact tattgccaac cattagtaac acccctatca
    15481 acaaatattt tctatttcag tatatcaaat agaaaagtaa aagtacgata gaaatttctg
    15541 gtcaattcta acaaatgatt tacattagtt caaaaacccc tcgactcgta accaaataat
    15601 tccc
//
LOCUS       DADVXO010000066        15535 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.068, whole genome shotgun sequence.
ACCESSION   DADVXO010000066 DADVXO010000000
VERSION     DADVXO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.068"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1835)
                     /locus_tag="KB950_001740"
     CDS             complement(<1..1835)
                     /locus_tag="KB950_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6857958.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="KB950_001741"
     CDS             complement(1832..2536)
                     /locus_tag="KB950_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6857959.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="KB950_001742"
     CDS             complement(2548..4857)
                     /locus_tag="KB950_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBB6857960.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="KB950_001743"
     CDS             complement(5052..5516)
                     /locus_tag="KB950_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857961.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="KB950_001744"
     CDS             complement(5516..6142)
                     /locus_tag="KB950_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6857962.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="KB950_001745"
     CDS             complement(6149..6514)
                     /locus_tag="KB950_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBB6857963.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="KB950_001746"
     CDS             complement(6504..6842)
                     /locus_tag="KB950_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857964.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="KB950_001747"
     CDS             complement(6832..7158)
                     /locus_tag="KB950_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6857965.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="KB950_001748"
     CDS             complement(7139..7417)
                     /locus_tag="KB950_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6857966.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="KB950_001749"
     CDS             complement(7419..8783)
                     /locus_tag="KB950_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6857967.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="KB950_001750"
     CDS             complement(8796..9365)
                     /locus_tag="KB950_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBB6857968.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="KB950_001751"
     CDS             complement(9337..10524)
                     /locus_tag="KB950_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6857969.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="KB950_001752"
     CDS             complement(10588..12315)
                     /locus_tag="KB950_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6857970.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="KB950_001753"
     CDS             complement(12312..12764)
                     /locus_tag="KB950_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBB6857971.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="KB950_001754"
     CDS             complement(12876..13256)
                     /locus_tag="KB950_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6857972.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="KB950_001755"
     CDS             complement(13253..13639)
                     /locus_tag="KB950_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857973.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="KB950_001756"
     CDS             complement(13641..13919)
                     /locus_tag="KB950_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857974.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="KB950_001757"
     CDS             13967..14695
                     /locus_tag="KB950_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBB6857975.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="KB950_001758"
     CDS             complement(14826..15302)
                     /locus_tag="KB950_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBB6857976.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
BASE COUNT         4359 a         3287 c         2426 g         5463 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatc
//
LOCUS       DADVXO010000067        15383 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.069, whole genome shotgun sequence.
ACCESSION   DADVXO010000067 DADVXO010000000
VERSION     DADVXO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.069"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(735..1076)
                     /locus_tag="KB950_001759"
     CDS             complement(735..1076)
                     /locus_tag="KB950_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6857977.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1076..1306)
                     /locus_tag="KB950_001760"
     CDS             complement(1076..1306)
                     /locus_tag="KB950_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6857978.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1593..1931
                     /locus_tag="KB950_001761"
     CDS             1593..1931
                     /locus_tag="KB950_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857979.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2136..2375)
                     /locus_tag="KB950_001762"
     CDS             complement(2136..2375)
                     /locus_tag="KB950_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBB6857980.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2351..2698)
                     /locus_tag="KB950_001763"
     CDS             complement(2351..2698)
                     /locus_tag="KB950_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBB6857981.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3430..4011)
                     /locus_tag="KB950_001764"
     CDS             complement(3430..4011)
                     /locus_tag="KB950_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6857982.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4194..4820)
                     /locus_tag="KB950_001765"
     CDS             complement(4194..4820)
                     /locus_tag="KB950_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6857983.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4842..6173)
                     /locus_tag="KB950_001766"
     CDS             complement(4842..6173)
                     /locus_tag="KB950_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBB6857984.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6328..6729)
                     /locus_tag="KB950_001767"
     CDS             complement(6328..6729)
                     /locus_tag="KB950_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBB6857985.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6815..7021)
                     /locus_tag="KB950_001768"
     CDS             complement(6815..7021)
                     /locus_tag="KB950_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6857986.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7116..8618)
                     /locus_tag="KB950_001769"
     CDS             complement(7116..8618)
                     /locus_tag="KB950_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBB6857987.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8636..9091)
                     /gene="argR"
                     /locus_tag="KB950_001770"
     CDS             complement(8636..9091)
                     /gene="argR"
                     /locus_tag="KB950_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBB6857988.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9189..9806)
                     /locus_tag="KB950_001771"
     CDS             complement(9189..9806)
                     /locus_tag="KB950_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBB6857989.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10027..11400
                     /locus_tag="KB950_001772"
     CDS             10027..11400
                     /locus_tag="KB950_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6857990.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11475..12587
                     /locus_tag="KB950_001773"
     CDS             11475..12587
                     /locus_tag="KB950_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBB6857991.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12659..14296)
                     /locus_tag="KB950_001774"
     CDS             complement(12659..14296)
                     /locus_tag="KB950_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBB6857992.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14464..15072)
                     /locus_tag="KB950_001775"
     CDS             complement(14464..15072)
                     /locus_tag="KB950_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6857993.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4247 a         3150 c         2681 g         5305 t
ORIGIN      
        1 atagtgtacc taaaagctct actgacataa gatcgtgtaa tcggtaaagt aattttatta
       61 attatacggg atgtaatcaa ttagagaata aactaatcat aaaaggtact tttacattca
      121 taattgtcgc tttgagattc tactttggtt cactatctga cccaattgca cattttcgat
      181 cattacaagt attggtgtcg ttagaacaac taaataaaaa ctagcggtag atttttcaga
      241 atctatgcta atttctgtat ggatgttcca gtgatggcaa atttctcttt tcattgtttt
      301 tcttgtcgca tgatatgcct ttttgtgtta ttcagttaaa ctatagaaat tctttcctag
      361 acagatcttg atcccaaatt ttacgttttt gagcatcgat atagtttaaa atgacattac
      421 tcatatctaa agagtttaga ttttttagga ctaaaactgt cagttgttct ttttcttctt
      481 tctgttaaag tattgtcgcg acttttcgta tacgttaaga tatgtcgtga aaaaacaagt
      541 tttgtgacac aaaaaaacga atgacttgag gaaatcacct caatcattcg tttaaaatgc
      601 tatatattct ttagtttaag atacggaaat taatgccatc gaccacacta gtagaatgaa
      661 cgacatttac ttggcctttt tttaatttcg ttatgtttag catataaaaa tattatagag
      721 tttacttaca atgcttaaaa catatactgt ataatctgat caattttagc catgtcttgc
      781 aatggtaagt tttctatttt tttgagtttt cgttccttaa aatcaagaga ttttaattgt
      841 gtaataatta cttgtccttt tatatctagc tcttcaggta atgagtaacg tgtcggtaaa
      901 ttctttatag tggaagtaat tggacatatg accgaaaaca ttgttttacg gttaaattca
      961 taacgagaga cgactagtcc tggtcgtctt ttttgaattt cttttcctac ggagggatca
     1021 aaatcaatcc atacaatatc acctttttta ggaatataaa ttctttcttc tgacactata
     1081 aaatttctct cccttcagga gttaaatctt cccattcgtc tttctcataa tactcggcct
     1141 cttccccacc actaaatgga tcttcaattt ttggaactaa cgtaattgtt ccatcatcag
     1201 aatacacaac gatatattct tggttttctg aaggtttctt tccgttatca gaaggtaaag
     1261 ttaccactac agaacttcct tgtaatctag attttgtaac aagcattatc atcactccct
     1321 taataaaagt atactatata acactaagta acgcaaaatt cccctttgta aactatctag
     1381 aaaattgttt tgtttaaagt atatcttatt ggatataaat agaaataaat tgtagccagt
     1441 tcattttttc tttaactaaa aaaataatcc aagaaacatt gaacctatgg atccaaaagc
     1501 tacccaaata ctatagctaa ccccccgcta ccttcaattt tgcagataaa acgctttggg
     1561 agaaaatttc aaagacaaca ggccatccta tcatgcccga ttataaacaa aaagctggct
     1621 attccttttg ctctgacaaa tttcaattgg gaaagttcac gaaactagca ggtgaagaag
     1681 tttcaactgt tcggatcaaa gaatgcccgc ttcaaataga aacattggtt caacgaattt
     1741 caactagaca agactttgct attgtagaac gtgagatcgt agctattttt gtagaagaag
     1801 ggattttaca tgatccaact catatcaatg tggataaatg gcaacctctt atctataaat
     1861 tcagagaata tacaactgcc aaccagtcgt taactttgaa ttttagattc caagaattca
     1921 aagagtcata atgataaaca tcgttaggta taaataaaaa agactcgcag cacgagtaaa
     1981 aaacaaatag aagctataca ttagaaaggt cgactgtgac cctatagatg cagtatatgc
     2041 agcttatgag catttcttct gttctctttg tttttgggat ctgtgctgaa gtctttttcg
     2101 ttttttgccg ctctttcttt gaaaaatcga ttatgttata ccttctccat tactaattca
     2161 ggcatctgct gagtaatgat ttttcgcgca aaagaaatat ctgttaccca aggttcgatt
     2221 tgttcttcag tcaagacaag cggcattcgg tcatggattg gtgcgactgt gcggttaggt
     2281 tcagtagtca ttatgattga ttctgccatt ttttcatcct gatggctacg ataaaaaccg
     2341 ccaatataca ctattttttc cttatttgaa aacataaatt tgtctttttc ttcattccac
     2401 tcattgggaa aacacaacga ttttgcaaaa atggttgatg aaaggtcttt ttttcttcta
     2461 ctgtctctgc acgggcatta atcatcaatt gtcctttttt aaatccggta aatccccact
     2521 tcatgatttc cggagtgagg atgttttctt cctcttttct taacgtcaca acaagatttg
     2581 acggaaaaac ttctccagct gctaattctg ctgcctgctt cggttgtttc gtctgggctt
     2641 ctcgataata attctgaagg agctctgact ctagatcaaa aaaataacga ccacacatcc
     2701 taatccctcc caatcttttt ttctatcata cactgcaaca agaagttcgc accaacaaaa
     2761 cgctttcaat ttttgtagaa ctaaaaaagt tgtgacaagt gacaaactct ccagtgaatg
     2821 gtattctcct gccaactcct cggtcacaag tcacaatatt caacaaacat gagaagctgc
     2881 tttttgaaat tgttccttgt gactcggagt gaaacggcag gcttacgacc tctgggtaaa
     2941 cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagct
     3001 attttcacaa aatttttctt aatactcgaa actaagcgac tttttcacaa cctcttcctt
     3061 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgagc
     3121 agctattttt atttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc
     3181 cactaaacgg tgttcaatca gctaaccttc ggtattagct caaaaaacgg caaaatatga
     3241 ggaactattt tgccgtttgt ttctctaata ctcaggtcaa agcgctgatt tcacaacctc
     3301 taatacacgg tgttccagaa gtagcttctt cgaaaaataa gccgaaattt cacaaaaatt
     3361 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
     3421 ctctaattat taaagaacct tttgcgaagt taacaatctt tctttgatca attgaaaatt
     3481 ctgcctaagt agtgatagat cctgtgtgac tttcaacaat aagatacttc cctggatttc
     3541 agcaatcaac gctttggctt gacggtctgc ttcttcatcg gacagttttt cttcagaaag
     3601 tgccaggata ttttgtttcg tatacttgac ccattgattg actaattcta gtagcaacag
     3661 acggtattct tcatccactg tactcaactc aataatcaaa ttacctacag gacagccatg
     3721 ataagtgatc tgtgtctcat gatcagcgaa aatccagtcc aacatttctt ctaatgcttc
     3781 ttctggctgt tttccagcat ttaagatgtc ttcgaacaat tctgtctgcc atacgtccat
     3841 atgcgcatgg atcaccgcta gacaaacttc tttttttgaa gaaaagtaat aataaaattg
     3901 accttttcct gtatcagaag ctgctagaat atctcggatt gacgtttcat gatagccttt
     3961 tttataaata agatctttgg tcgcttccaa gatcttttcc tttgttttca atgatattct
     4021 cctttttcat ttctcatagt cctatattat acagtaaatt catcattttt tcttcttaat
     4081 cgtttaaaaa agactgtgcc aaattatctg acacagccct cactataaaa ctgttctcat
     4141 atagctagat gttcattgat tcaagaactg cgaggacaaa agcaattcct atcttatgat
     4201 agttgctcta atacttcctc tgttgtcatc acttgatctg caataaagcc aaagttcgtc
     4261 aacgctgcat ccattgcttc ttggccagga gcaccaattg catctgtcgc taccacaaca
     4321 ttgaatccac tctctaccaa ttcacgcata tgggaatcgc tacacaagtt tccgttcatt
     4381 ccaccaagaa ttactgtgtc gattccattt tttctcaatt ggagcgccaa atcattgctt
     4441 tctggtccaa agattttatg aggagagctg atgatcgtat tttcatccaa ataaggtttc
     4501 aattcttcga taaaatcggc tcctgaacct tcttccactg attcatattg actttttcta
     4561 gcaaacatct gctgctcaag catcatatgt tctcctgcac cgccaaattg ccatttatgg
     4621 tcatgtgggt aaaaataatg aggagaaata aagagtttgt atcctttttc ttttgcttgt
     4681 tttaccattt caatcatatt ttcgatcgta ttgtattttt ctaagatatc ttttgtcaaa
     4741 ccaaagccac cgcctgctgg tttcaagaac tcgacttgcg ggtcagtgag aactactgct
     4801 gtatttttat tattgaacat ggtggattct ccattcgtta tttattttaa tacatcattt
     4861 agagcttcac tcattgtcgg atgagaatag atttgatctc tcaataacgt ataaggaagt
     4921 tttccattca tagctagcga aatcaagttg atgacttcat gagaatcttc cgcatagata
     4981 cttgccccta gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac acctttagga
     5041 tcttctaata cttgtgcttt agggacaccg cttgtcatat acttgaataa tttgtaattg
     5101 acgccttgtg ctttggcttg tttttctgtt aaacctacat tggataaagt cggagtgata
     5161 aatacactgt aaggcacagc tttccggtta ttcgttgttc gtttattctc gcccttcaac
     5221 tgatccataa tgattcgata atcatctaga gagatataag tgaactgcaa tccgcctttg
     5281 acgtctccaa tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc cacagcgatt
     5341 gctccgcgat ctgttagttt aacatctgta ttttctaatc caagtgatgc tgtacttggt
     5401 tttcttccag ttgcagccaa aacttttgac gcttggattg ttacttcttg gccatcttct
     5461 gtaaaggtga cggcagctgt tgattcttgg tctgctactt gatctacaga tacacctaga
     5521 tggaattcga ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat atcttcatct
     5581 tcacgtggaa gaaattcaga atgggcatca agaacgacaa ctttagatcc gtattcattg
     5641 aacattgatg caaattccaa gccaatatat ccagccccga tgatcactaa tttttccggt
     5701 aattgttcct gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt attcaatcct
     5761 ttgattggta aaatgattgg ttcagaacca gtattgatga aaatccgttc gccttcaaca
     5821 atcacttttt caccattatt cgtcacttcg atttgatggt tagaaagaaa acgtgctgta
     5881 ccgtccatca ctgtcccagt tgcctcatcg ctgatcatat gataattttt ggcgcgaagc
     5941 atgcccgtca atttttcttt ccgagcaaca gcctctgtaa agtcgattcc tctttctcca
     6001 ttgaaaatca atgatttaga aggaatacag ccgatgttga tacatgttcc gccatacata
     6061 cgtgttgact tttcaacgac taaaaccgat tcaccttttg aagctaacgc cttcgctaaa
     6121 gtcttaccgc cctttccaaa accgatcaca atattttgat atttttccat catttttccc
     6181 tcgcttcttt tttacctacc gattggtaca aaaatatgat aaccgataat tgtctattct
     6241 ccaaatcatc tgctttattt gtcaaaaaaa accagacaaa aagcggtcct ttcacgtttt
     6301 tagtctggtt cgatttcctt tattcttcta aacaatcgtt acttctacat cgatatttcc
     6361 tctagttgct ttggagtaag ggcagaattc atgcgcatct tctgcaattt tttgggcttc
     6421 ttcttcgctg atgccatcga tatgcacttc taatactaca ccgatctgat agccgccatc
     6481 atctaaatta taaagagaga cacgtgcttt tactgtggac tctgctgtca ttcctgcttc
     6541 tttgatcata tgttgtaccg ccccattgaa acaagaagca taacctgctg caaataattg
     6601 ttctggattt gttccttctg catggattcc tggaggagtg attttcatat gcatcgagcc
     6661 atttggtgcc tcgacttctc cattgcgccc gccatggttg atcattgctg tttcgtatgc
     6721 ttttttcatt gtactgctcc tttcgattga ccatttgcta cttggctata attatagtgt
     6781 atgacaagta aggcgctttc tacaaaaatt ctgcttattc gaataccaga atgccgtctt
     6841 ctactatttt tccaaaaaac aaaaaaatac tactcgtgaa caacagcgtc atatttgttc
     6901 caaaataaat agcaatcttt tctacataat cttttatatg attgatttta agatacatat
     6961 ataccagaaa gttgacccca taaaccaata ctgttaaaaa aataaccata ttcgttacca
     7021 ttttttcctc cttttgctgg tctccctacc agcactcgat ttaaccatac cacttttttt
     7081 gccgtccaag caactagcct ttcaaaaaga ctggtttatt ctgctgttaa tgattcaaga
     7141 tcagaaaaat gtccgaataa ttttgctaat tttactaacg ccaacttgtt ctttccgaat
     7201 tcatcatttt ctatctgtaa tactagtaac ggctcatgta agcttaaacg gatcaaaaac
     7261 cagccgtttc cgtatggctc ttctagatca tatcgaatac cttcttcatt ttccgggtcg
     7321 aagttcatcc ctttttcttc ttccaacagt cgcttcattt cttctaatat cttgtttcct
     7381 tgttcgcgtg gttcatcgct gaacaatttt aatcggattt cttgtacttc tacgggctgt
     7441 ttcaactctt tgatttgttc atctaatgtg ataccttttc tttttaattc tggcaaaagc
     7501 atcaggatct ttgcgatgac atatgcacca tcatccagaa aatagttttc cttgaatgca
     7561 gcgtgtccgc ttgtttcgat ggctagctgt gtatctttcc caagacggtt aagttctaat
     7621 gctttattga ttacatttcg atagccggaa atataacgaa cttggatgcc tcctttttct
     7681 tcaatgaatc tttgtagatg agaagaagta ggggaattcg tcacgattct tgttcctgga
     7741 tgctctttta atacgattgt acttaatact gcgatcaaat tatttcgatt gatcactgtt
     7801 ccagaagcac tgacaactgc agaacgatcc acatctgtat cgaaaatgat cccaagatct
     7861 gcttcatttg ctaaaacagc ctgcttgata ctttccatcg cttctttatt atcaggattt
     7921 gggatatgat taggaaatcg gccatctggt tccaaaaatt gggaacctgt tgaatccgca
     7981 cctaagctat tcaatacttt ttcagcaaag aatccaccag caccatttcc ggcatcaaca
     8041 atgattttcc aaccatttag tggtttgctt tctagtgttc caatcccctt tttaattttt
     8101 gtgactaaat cttgcgcata aggtgtcaaa aggtctgctt tttctacgct tccagtttgt
     8161 tcgcttttta ttttttgctg ctgtgatagg ataaaagcaa tatcttcttt ttctgctcct
     8221 ccttctgagg taaagaattt tattccatta taataatagg gaagatggct agcagtcagc
     8281 ataaccgcag catcgcacga gaactgttca aattgagtgg acataaacat agcaggcgta
     8341 gtagctaatt cgaaatcgat gacatgtatc ccttgttctt tcagactttg gatcaacgcc
     8401 tctttcagtg ctggtccgct tatcctactg tcatgaccaa tcccaatcgt ccatttttta
     8461 tttggatcgg ttgatttctt ctttttcttt atccagtttg ctagtcccca gccgatcttt
     8521 tttaccatgt ctgtcgtcaa gtttacttca tatccttcaa ctggtaatgc gattcctcga
     8581 atatccgatc cattttgcag cgctttcata tctttcatat aaaagtcctc ctcatttatt
     8641 ccctcatttt ttccagcttt tctgcaaatc gctgtgcttc ttcttctgag cggcaaataa
     8701 taatgaatgt atctacaccg gccatcgttc cgacaacttc agggtaagcc acttcatcta
     8761 gccaattcgt taccacatcg gcaccatttc gttcagtcaa tacgataacc atgaactgaa
     8821 cagactggat acgcaaaacc tcgcgtctaa ttgctgtacg caaacgttct tcatttaaag
     8881 aaacagatgg ctggttaaat aatgtatagc gtactatttt gttttcattt tgacttttta
     8941 ctaacttcat ttctcgaatg tctcgagaaa tcgttgcttg agtcacatcg gcgcctgctt
     9001 catgcagccg cgcaagcagc tcttcctgtg tcccaatagg ttgttctgta atgatctggc
     9061 ggatcagtgc ttgtcgctgt ttctttttca tattagacac ctctcaatgt tgattatagc
     9121 aaaagataag taatatttct tcatattttg gtatttttta cttttatttt ttgttttcat
     9181 tcaggttgct aacctttttc tattttaaca cgcaaaatcg tcttcatttt ttcaggtgct
     9241 gattttcttg gatcgctgat gtaaatctct ttgtgttctt tagaagctct ttcatagcct
     9301 tcttttgcga tgaatgcttc taatttctca aacgttgctg gttcttcggc atagggcccg
     9361 acgtgtaaga tttgggcaca aagaccttct tccatctttt tgaatactac ttgttctttc
     9421 aagtcttccg gaagtttgct agaacgctga atggcttctt gtgctatcgc ttctgtaaca
     9481 aagtcaggct gtcggatcat caattgataa gaaaaatgtt cttttttcat cactggttca
     9541 tgcaaatact tttcttgcag tccccattgt ccttccagtg gatagacagt atacatctga
     9601 taatcaggaa tcagccaatt ttttttaggc gacatgcgta ttgcatagct gactgcatat
     9661 agacaggcta ctcttcgttt aaagtcttcc tgattcgggt cgccgatccc tctgatgcta
     9721 aagaatgatt ttttaggaac ctctagtatt tgcgggcttt ttttcatact atagctcttg
     9781 tctttacgcc attcaatttt ttccattttt cttcctccgc gaaattccat ttagttattt
     9841 attatcatac ctctttttga gtgacttggc taatcgtgtt gacactattt ttcagtttgt
     9901 gctagaataa gtgaagtaaa taaatcgtac agataacacg tttcggcgtg tcgagagaga
     9961 ggcggtttct atttttataa tagaaatcac tctctttttt tacgaaattt tttagaggtg
    10021 atgtaaatga caaaacaaaa acagctgctt ttagtacctg cagtaattgc aacagggatg
    10081 atgtcttttg cgggcgtatt gatcgagacg gccatgaatg taactttccc aactcttatc
    10141 agacagtttg atttaacgac tgcacaagtc caatgggtaa caacgattta tcttttgatg
    10201 atttcgatca ttgttccgct gtctacttat ttgaatcgca attttagtct caaaaaactc
    10261 tttttagctt caaatctgct tttcttggca ggtataatga tcgatttctt ctctcctacc
    10321 ttcatggtcc tattgtttgg tcgattgctc caaggtgcag cgacaggtat cgctcttccg
    10381 ttgatgttcc atattatctt gacatttgcc cccctcaatc gacgaggcgc gatgatgggt
    10441 gtcggtactt tgacaacggc cattgctcca gcaattggcc ccacttatgg aggcattatg
    10501 acttcacatt ttacatggaa tcatatcttc ctgtttcttg tgcccgtttt gtttctttct
    10561 ttgattatcg gctggtttgc tattcccgag atggcagtac aaaaaagtgg ttatttggat
    10621 tggataagcg ttttaggaat ttgtcttttg tttagtggaa gtctaacatt tttgagtacg
    10681 ttgggaacag tttccagctg gattgcttta atcatcggcg gtattggctg gttccttttt
    10741 tactatcgaa gtaaaaaagt ggagcatcca ctgatccgac tgtctattct ttcaaataaa
    10801 acattccgct tgtttttgat tggcttctta gtttatcagt ttttattact tggtgtatct
    10861 tttgtcttgc cgaatttcct tcagatcgtt caaggaagta gtccttttgt cgctggatta
    10921 gcgatgcttc ccggcgcagc ggttggtgct ttgctttctc ctttttccgg gaaaatgctg
    10981 gatcaatatg gtccgaagaa gccaattttt gctgggctga ttctttcttt gattggttgg
    11041 ctagcattat tcctactcat aggacacgct tcagtgggtg tattgattgc ttgtcatgtg
    11101 ttttatatga tcggtatcgg attatcttac agcaatatga tgaccaccgg aatgaatgca
    11161 ctgaatgaag aactacaagg agacgggaac gcaatgttca ataccttgca gcagttttct
    11221 ggcgctgtgg ctacttcatt agtagcggtc attatcaatt acgtccagca tcatacttcc
    11281 cataactatg aagtgtctac tacattagga tcaaaagcag cattaggcgt cttgttgctg
    11341 ttgcttctcg taagctttgc tcgattcgcc tattatctgt tctttgctaa aaaagcgtag
    11401 attccatata cggcaggtgt tgcatctgcc gtttttttgt gtgaaaatgg ttgtttgtaa
    11461 aaggaggctt ttttatggaa aaaattgcca ttatcggtgg cgggatcatt ggtatgaccc
    11521 tagccagtta cttagataca tcaaaattca atgttgcttt atttgatgaa gggacaggac
    11581 aggcaactaa agctagcgct gggattatct ccccttggtt gtcgaaacgc agaaacaaac
    11641 aatggtatcg tctggctaaa gacggcgctg cttttttccc taaacttata aaagatttcc
    11701 aacttgatca aacgatttac gcacaatcag ggacactttt gcttcgacct gaaaaagatt
    11761 tagctgaatt ggctgttctt gccgagaaac gaaaagaaga agcgccagaa attggaaaca
    11821 tcgaatggct ttcacctgaa cagacatctc aagcacttcc gttattgaaa ccagcagttt
    11881 cactgcgtat cagtggtggt ggtcgtctgg atggaaaagc atatttagaa catcttggtc
    11941 agcgtttagc acaaaaacaa atggagcaga tccaggcaaa agtcactttg cataagaaga
    12001 gcgattcttg gctcatcaag ggaccgtttg gagaatacac agctgatcgt gtaattttga
    12061 cacctggccc ggcacttaag cctttattac aagaattagg ctatgctgca gatatacgtc
    12121 cgcaaaaagg gcagctctta gcatttcaga catcttataa gatgagtcaa gactggcctg
    12181 ttgctatgct tgacggagaa gctgatttca ttcctttcat ggatggaaag atccttttag
    12241 gtgctaccca tgaaaacgat caaggctggg atttggctcc tactagcgaa gcttataagg
    12301 gattgactaa gagtacagaa cagttcttga ctgatccaga tcagataaaa aactggccat
    12361 acgagttccg tgtaggtacc cgtgcttata cgtcagattt ttccccattt ttcggcgtac
    12421 tgccagatga tccaagctta ctggctgcta gcggtctagg ctcttctggc ttgaccacag
    12481 gtccttatat cggctatcta ttagccaact tcttaaatac tggtcattgg cctacggaag
    12541 attatcaaaa ggacatctcc acctatatta cgaaaaatcc atcatagaaa aacagctttt
    12601 tcaagtaagg atcccgagcg atattgcgca tggattactt cttgaaaaag ctgttatttt
    12661 agttcaattt ctgaatcaaa ttctccaaac tttcttttgc ttcatctggc aagagatctt
    12721 ttcctatctg agcttcaatg aatcgtaaac gaatatcaag tcgttgtttc accagatttt
    12781 tataggacgt atcctcaaaa gaaacaggaa aatctggtat aggtaaatag tggatgtctt
    12841 caaatggacc aaacgacttg aattctgccg tttgttgcac gattctttca acgattgaca
    12901 atgtcgtttc aggtttgatt tttttatcat cgaaatatcc agtattcagg acatagcagg
    12961 ctgtttgctg tttcaaaaac aactctcgaa aagcttgata gtcttcccta agaggataga
    13021 cacggaaagg attagcaaat ggttcaatga ctagctcatc tagctgtctt tttgtagtga
    13081 tattttccgc gtctgatcgt tttgttgcta atgttgcacc aaaagacgct gctaaaactg
    13141 ggtcattgat cttgataatt ggcggaagcg aatcatcttt catgatccaa aaaacagcat
    13201 cgatcttttc gctaagatga tcgactcggt cgggtgtcgc ataacgtgat ttgactgtcc
    13261 gcccatttcc gtttcgtata tctcctgtta ctaggacttt ttctccttta cttgtacatg
    13321 tcacgcccac attctgacaa gtaaggaaag aagaaatggc cggatcatca gacggatagt
    13381 cttgtgtttt atcaaagtaa gaaggttcca aggcagtagc aaaaccattt tttcgagaaa
    13441 taacgaaagc atcgtcatgc aagatcgaaa cagagtgacc ttccgtatgt tttgctaagg
    13501 taatcgttga ttttccagaa ccggacaacc caaatacagc catcgtatag gtctggtcat
    13561 tactagcata ttgtttcatc ccaccgtggc aggcaataaa gccattgcga tttcccaatg
    13621 cccaagcaag cgtcaaagtc gcttttttta attctccaaa gtaacgtaat cctagcacag
    13681 ccgctgtatt atggagagca tcaaacaaaa tgagtccatt tggaaatgtt tcatgtgtcc
    13741 attcgggatc acaaaagata taaagatctc cctcatcaaa ggaaacagac ttttgataag
    13801 tctctaagat cttcgaattc gctggctgaa aattcagcaa ataagaatac aaattgttct
    13861 caaatccttc tggaatcagc aaatgacttt gcacagaaaa ttcttcagat aggccaacga
    13921 ttacttttgt atggtaaaac tggcgcttgc gactttgata cacagcttct cttaaaattt
    13981 tctcgtattt gctttgttcc ttgtctaagt cctcaaagat ccgccgagca cttgctgttc
    14041 ttcccacaat gcttccatca ttgaatacta gtgtcctaac atcagctggt aaatctagtt
    14101 cttcagcatg tgagattgtt aaatctgtga tgatcgttcc tggactccct tttgctagtt
    14161 gataagctgt cttcctatca aaaacttcat gaacattatt accgtaaaat gtcgtttcta
    14221 caatcgatcg aatactggat aacagtggat ttccacttgt gatttcgttt aattcgagac
    14281 tctcttttgt actcatggct cctcctcatt ccctaaacaa aaaaacgttt tctttattat
    14341 acattattcg agtttttatt tgtactgctg cgctgtggga agcaaacgag ttatttttca
    14401 cacaaaaaaa ccgagaactt gaataaacaa gtttctcggt ttcatcattt tccttttatc
    14461 ggcttagtac cagccgtttg ctaaccagaa tgttttagca gcatcccatg aaccataacg
    14521 agatgttaca tattgttctg ctacacgttc ttgattggca gctgaatagt cgccgtttaa
    14581 gtatgaagca tctaattgat aacggccgat gtaacgtccg ttagtagctg tgtatgaacc
    14641 gcctgattct ttttgtgcga tccattcttt tgcagagctt gaagttggcg ctgttgcagc
    14701 agctggggca gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg tatgttcttg
    14761 cgttgctgtt tcagttgtag atgctgtatt ttctgttgtt ttgttttctg ttgttgctga
    14821 agaatcattt gaagttggga ttgttaattg ttcaccaaca taaatgcggt tgatgtttgc
    14881 gatcgaattt ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa atttttgtga
    14941 gatcttagat aatgaatcac ctgattttac tgtataaact tcatcagcgt gagcatttgt
    15001 agttcctaca aacattgcag tagcaccagc agcaaaagtt gtacctaaaa caattttctt
    15061 aaatgaattc atattaaact cctttttttc tccgtttttt attctttcga cgctattaac
    15121 tttatcattt agaaatattt cataggtaaa agaaacatga catttaatga caaaataacg
    15181 aattatttaa tatttgtaac atttaaacaa atattttttt gatacaaaac cataaaagcc
    15241 ttgttgtata agcaaagtaa tcgtttttct atgttttttt atttttgaat gaaaaacgca
    15301 gattctgtaa cattcactgc aataatccat aaaaaaaagc aaaaaaattt ttgattttta
    15361 tcaaattctc ttaaaaaaat gta
//
LOCUS       DADVXO010000068        15210 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.070, whole genome shotgun sequence.
ACCESSION   DADVXO010000068 DADVXO010000000
VERSION     DADVXO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.070"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(94..1137)
                     /locus_tag="KB950_001776"
     CDS             complement(94..1137)
                     /locus_tag="KB950_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBB6857994.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="KB950_001777"
     CDS             complement(1514..2479)
                     /locus_tag="KB950_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBB6857995.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="KB950_001778"
     CDS             2713..3558
                     /locus_tag="KB950_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6857996.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="KB950_001779"
     CDS             3749..4936
                     /locus_tag="KB950_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBB6857997.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="KB950_001780"
     CDS             4942..5298
                     /locus_tag="KB950_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBB6857998.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="KB950_001781"
     CDS             5300..5638
                     /locus_tag="KB950_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBB6857999.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5714..5970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6033..7067
                     /locus_tag="KB950_001782"
     CDS             6033..7067
                     /locus_tag="KB950_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6858000.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="KB950_001783"
     CDS             7060..7746
                     /locus_tag="KB950_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858001.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="KB950_001784"
     CDS             7770..8618
                     /locus_tag="KB950_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBB6858002.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8815..9588
                     /gene="sufC"
                     /locus_tag="KB950_001785"
     CDS             8815..9588
                     /gene="sufC"
                     /locus_tag="KB950_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBB6858003.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9601..10887
                     /gene="sufD"
                     /locus_tag="KB950_001786"
     CDS             9601..10887
                     /gene="sufD"
                     /locus_tag="KB950_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBB6858004.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10884..12119
                     /locus_tag="KB950_001787"
     CDS             10884..12119
                     /locus_tag="KB950_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6858005.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12106..12576
                     /locus_tag="KB950_001788"
     CDS             12106..12576
                     /locus_tag="KB950_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBB6858006.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12581..13972
                     /gene="sufB"
                     /locus_tag="KB950_001789"
     CDS             12581..13972
                     /gene="sufB"
                     /locus_tag="KB950_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBB6858007.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14070..>15210)
                     /locus_tag="KB950_001790"
     CDS             complement(14070..>15210)
                     /locus_tag="KB950_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6858008.1"
                     /translation="ATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAEA
                     QTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPA
                     FGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLL
                     PRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDI
                     DYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMIL
                     YGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFE
                     SGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4973 a         2839 c         3250 g         4148 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat ttagaaatca
     8701 aaattgttta ataataaata aaagatattt gattttagta aggaaggtca ttgcgcttca
     8761 ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata atgcatgtct
     8821 gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta
     8881 aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg cacaggaaaa
     8941 tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga aggcgaaatt
     9001 ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg acttggctta
     9061 tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga attcatgcgt
     9121 gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc atttttgaaa
     9181 aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga acgttatctg
     9241 aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta
     9301 gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga tgctttgaaa
     9361 gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc
     9421 cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat gatggaaggc
     9481 cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga aggctacgct
     9541 ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg gggagcaaga
     9601 atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc tttaagtaaa
     9661 ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc agatgaactg
     9721 ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga tgtgaaagaa
     9781 acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa agacaatcct
     9841 gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga attagcagaa
     9901 aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga actagtccaa
     9961 gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc actccatgta
    10021 gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca
    10081 ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa gcatgtgttg
    10141 atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac aaccaaagaa
    10201 caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc tggttcaaaa
    10261 atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat gaatcgccgt
    10321 gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat
    10381 gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga agtcaaaatc
    10441 gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac caataaagcc
    10501 cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg aacattgacc
    10561 tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt
    10621 cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct tttgatcgat
    10681 gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc agaagaaatg
    10741 tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt gattcgagga
    10801 ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga attagtcgaa
    10861 gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat gattttccta
    10921 tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca gcgacgaccc
    10981 aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat aacgccaatg
    11041 ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa caagcaagag
    11101 aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt actcgtggta
    11161 cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc aaagcaggag
    11221 acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg cagcagttgg
    11281 caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc ttcctggata
    11341 tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt gctcatgtat
    11401 ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc catgaaaacg
    11461 gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt gatgtccaag
    11521 cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca acaggtattg
    11581 gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa tttggtggcg
    11641 aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct tggaaattcg
    11701 aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc gattatttgc
    11761 aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct tatgtgttgc
    11821 caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg aattttcgga
    11881 caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg acagctttag
    11941 acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc
    12001 ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg caagacgccg
    12061 accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg actatctaaa
    12121 ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca tcatcgtgga
    12181 agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc
    12241 gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga cggcagtggc
    12301 tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata
    12361 gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa
    12421 gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt tcccgctcgg
    12481 atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa cgatggtacc
    12541 gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac cagaattaga
    12601 agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag gagaaggctt
    12661 gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat ggatgcttga
    12721 attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat ggggccccga
    12781 cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg acaaacccgc
    12841 tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga ttggtatacc
    12901 agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta
    12961 ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc
    13021 tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac cgccaaccga
    13081 caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta tttacgtgcc
    13141 aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg cggaaaacac
    13201 ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc actatgtcga
    13261 aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg tagaaatctt
    13321 tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag acaatgtgta
    13381 caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat ggatcgacgg
    13441 aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc
    13501 acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag atacgggtgc
    13561 aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat caatttcaaa
    13621 agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc
    13681 gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat ccgatacgat
    13741 cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca aagtctcaaa
    13801 aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat ctgaagcaac
    13861 tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa tggaatatgc
    13921 agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt aataactatt
    13981 tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt tttttatttg tattttaatg
    14041 gttggctacg tttttggcta cgtttggaat tattcgccca taaattttgc aaatcggtca
    14101 ccagttttct taacagattg tggtgtgaca tgagcataga tattcatggt agttttaata
    14161 tctttatgac ctaatcgttc ctgtacttct tttatcgacg caccactttc aaacaataag
    14221 ctacagtggg tatgcctaaa tccatgtgga gtaatttgag gctccatcgg tgtctttttg
    14281 taaatccagt ctaaccaatc gtttactact tgggggtagt acaacttgtt tgtgtttgta
    14341 gtgaaaagaa actgttttgg tgaattcgtg ttatagccat ataagatcat tattttcatt
    14401 tgttcttgtt gccaaatacg cagttgttta agtgtaaaat catcaagctg aatcgttctt
    14461 gatgaatttt tagttttagg tacttgtaga actacttgat taaactcgtc ttgagcgata
    14521 gttttaccaa ttgttattgt tttattgaaa tagtctatat cttcccattg aagcgccaat
    14581 gcttcgcttt tacgcattcc tgtataagct aataaacgga aaaaagtgta cagtttaact
    14641 gaattatttt ctttagtatt ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc
    14701 aagggttgtt cttcttcaat ttttcttgga agcaatgttt tagacatagg attatcctta
    14761 cagacttcca ttgctactcc aaatttaaat atttgagcag ttgtttttct caagaaacca
    14821 tattgtttta atgttttgtg ccattcattc acctgttttt ggcaatagct aacacttatt
    14881 ttatccaatc gtaattcccc aaaagcaggt aatatatggt ttttgcaaaa acgttgagca
    14941 acggcaacag aagacggttt tactcttgtt ctgtagttat caatccataa ttcatacaac
    15001 tcttgaaaag ttgtaacatt gattttaaac tcttgttttt ctttaaagtc tacaagtagt
    15061 ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc ttctacgctt tgtacgtata
    15121 tcttttccag tcttgggatc tttccctaaa taagtttcaa atccccaata tttttcgcca
    15181 ttttgtaatt tataactaaa aaatgttgcc
//
LOCUS       DADVXO010000069        15052 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.071, whole genome shotgun sequence.
ACCESSION   DADVXO010000069 DADVXO010000000
VERSION     DADVXO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.071"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            217..1119
                     /locus_tag="KB950_001791"
     CDS             217..1119
                     /locus_tag="KB950_001791"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBB6858009.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            1332..3107
                     /locus_tag="KB950_001792"
     CDS             1332..3107
                     /locus_tag="KB950_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBB6858010.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            3364..4581
                     /locus_tag="KB950_001793"
     CDS             3364..4581
                     /locus_tag="KB950_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6858011.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(5028..6614)
                     /locus_tag="KB950_001794"
     CDS             complement(5028..6614)
                     /locus_tag="KB950_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBB6858012.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            6865..7089
                     /locus_tag="KB950_001795"
     CDS             6865..7089
                     /locus_tag="KB950_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBB6858013.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7231..8982
                     /locus_tag="KB950_001796"
     CDS             7231..8982
                     /locus_tag="KB950_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6858014.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            8982..10766
                     /locus_tag="KB950_001797"
     CDS             8982..10766
                     /locus_tag="KB950_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858015.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11224..12075
                     /locus_tag="KB950_001798"
     CDS             11224..12075
                     /locus_tag="KB950_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6858016.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            12172..14058
                     /locus_tag="KB950_001799"
     CDS             12172..14058
                     /locus_tag="KB950_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6858017.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14099..15001)
                     /locus_tag="KB950_001800"
     CDS             complement(14099..15001)
                     /locus_tag="KB950_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6858018.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         4788 a         2763 c         3099 g         4402 t
ORIGIN      
        1 agacaaggct gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga
       61 tcttttttat aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga
      121 acagggcaaa gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt
      181 gttagtaaca attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc
      241 atgagcggaa agaaaatgct gttaggagca gcagaagaag cagccagtta tggatcttcg
      301 acttttatga tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg
      361 aatattgatg ccggaaaagc gtacatggca gaaaacggtc taacaaatat cgtcgttcat
      421 gccccttata tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc
      481 caatttctga gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt
      541 cacccaggtg ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc
      601 ttgaatgaag ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt
      661 aaaggtacgg aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa
      721 ttgaatgaaa aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat
      781 attaaagaag acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg
      841 cttaaagtac tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac
      901 gccaatattg gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca
      961 atgtttgaca atatttcgaa aatcttggaa actccctatg tcggcaaaga taaaaagaaa
     1021 gcttacgcac catatggaaa agaaatcgcc atgttcaaag cacaaatatt cgatccagaa
     1081 gtgtttcctg agttattagc agaagaagtg atactctaag attttctatc tgacagcagc
     1141 aaaaaaggac caggcacatt gccttgtcct tttttgttta agaaacaaag gagtgctttt
     1201 tgcagtcttt ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacagggta
     1261 tactggtaga aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga
     1321 aggacgaaaa gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata
     1381 catactttat tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta
     1441 aagcaatcta tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg
     1501 ctcaaaatat tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag
     1561 gaatctccta cagcttattt ttagtacttg cacataaact gcatctttct tatgcagttt
     1621 tgttcggcat gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgatcg
     1681 tgaagaacaa gtatcttctt ttaagtttct atttttattt tttgttttct ccgattagtt
     1741 ttacacatga atattccact cgaacgtatc gtaatgccct cgtgattccg gctgttgaga
     1801 tcattatcgc ttgtttacta gctatttatt accgtaaata tagttccagc aaaaaaatgc
     1861 tgccgtggtt tttagggcta gctgtttctt ttcctttctt ttggttcatt cgcgaagatt
     1921 ccatctggct tcttcctttt actgtagtcg cgttaggcat cagtttcgtc cagatcttgc
     1981 taggacaaga cttttctttc aaagaagcaa agaagttaaa agaacgacca tttctccttt
     2041 cgaaacaaga atggctgaaa ctttcattgt ttttactccc tctagtcggt ttctccatcg
     2101 tctatcagtg gatcgaatat aaaaacgaga caacatatag tattgccgcc atcaatgacc
     2161 gttcgagtgg cgaatttgga cgtttgatga aacagctgat ccgaatagat gacgaaacga
     2221 acttaaataa agaaaacagt gagatatggg tctcgcatgc tgcattaaat aaagcattag
     2281 acgcatctcc tacatttcat gctgtttcta ctcgtattga cgaattgtat acgactcatg
     2341 cgtggaccca aggtggaaaa gtaaaagaat taccaggaga tatcattttt tgggcattgc
     2401 gagatgtggt aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt
     2461 ggaaaaaagt caatcaagaa ttagaagaag cctatcaaaa gggaactatc aaaaagaaaa
     2521 aagaattcta tctgatggga tcaggggatg gaaagacgat gaatgatctg ccaatcgtat
     2581 ttgcttttct gaaatcagga tatgcctatg catggcgata caaagatttc catcaagggg
     2641 ggaattatag tttcggtacg caagaacaag tcgaacaggc ccaagaatta ttgaatcttc
     2701 cactaatgga taattggatc gatagcagtg atccgagaac acctgatttc cagttgacaa
     2761 aaacagcaaa agtcgcgaat gtcatgataa aaatttacca agttctggcc attcccgtgc
     2821 tgattgcttc aatagcaggg atattactta tgatactagg aaccgcattt gcaaaagctg
     2881 atcgtgccta ttatagttcc tctttgattg tagtcaccgg attaatactg acacaattgg
     2941 tttttctttt cggcgtctcg tggttttgtt cttatgcgcc agaaaaaaag gattattttt
     3001 taagtgtcta cactggcgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc
     3061 tagttttcag taaattgaat ctgaaaaaag ctttatcttc caaataaaaa catactaaga
     3121 ttagaggaga acatcattga cgaatgatgt tctcctctat tttctatttt tagattatag
     3181 tgaagagtga aagaaagttc gaacaaagcc ttgaggacta tgagtcccta tcaaaaaccg
     3241 aacttcttta ctgtcatttt agaatcaggt ttacgtatgt tggggccatg agcccgtgta
     3301 taggaaattg gaatcgaaat gaagcagtaa agttctttca aaacttaaaa aggaggaaaa
     3361 caaatgcctt acattcttgc attagatgtt tcaatgggac acagctacag tgtcctatac
     3421 aaagacgaca cctgcttatt tgaagacgag atcgaacaca ctcaaagagg atttcatgca
     3481 ttactcgaag agatccagca cttgcctgaa cgtccattga ttgtttttga agcaacgggg
     3541 atttattcca gaccagtcga aaagttcttt caagatcatc acttttctta ctgtttgtta
     3601 aacccgttag aagcgaaaaa acagatggaa gaaaacacct tatgcagttg gaaaacagat
     3661 aaatcggatg cccatcggct cgcacagact catcttaaaa atcaacgtca gccaaaagaa
     3721 gtacaaagca atttttactt agagatgcgc gatttagccc gtttttatca agaaattgag
     3781 aagaaaatca ctcgtctgcg catggattta cacaactgcc ttcagttgac ttttccagag
     3841 cttgaacagt tctttttaaa caggctgacc ccttacgcat tgatattgat ccgtcttttt
     3901 ccccatccag attttgtatt ggcatctact cggacaaaaa taaaaaataa actgatcaac
     3961 gaaacacgga aaaaaatctc cgcgaatcgt gccgagcaaa aggcagatca aatcatccac
     4021 tatgcacagt gtgcctatcc agctgttgaa aaagacagta tccactgtca aaaaacgatc
     4081 tattacgctg aacttctcca agatttactc gaacaaaaag aagcactggc gacacaaatg
     4141 atcaaaaaag ccgaaggctc tccttgcttt ttgttgtacc aaacgttccc tgggattggc
     4201 gcactcacag ccgctttatt gctcggtgaa ttaggagata tcacacgatt taaaacccat
     4261 aaacaattga atgcctttgt cggaatcgac atccgacggt atcattcagg taagtatact
     4321 ggccaagacc gcatcaataa gcgtggtaat cccaaagcac ggaaaattat tttcttcacc
     4381 attcgaaata tgattcgcca gcaacgcgca gctcctaatc atattgtcga ttattactac
     4441 aaattaaaaa agcaacctat ccctaagaaa gataaggttg ccacagttgc ttgtatgaac
     4501 aagcttctga aatgtatgca cgctatggtt agggcacata cagagtacga ctatgcgtac
     4561 gcggtctcag tggaccatta actaaaccac ttacagtata ccacttcttt gcccagatcg
     4621 aaaatttttt acgaacgggg ctttatttgg tgtgccttga ttttgtcgct ttcgtttatg
     4681 agctattttt cttacacctt ttacttgact aatcgtagga aagggaactg tgacaaaagt
     4741 cttgtcgcag ttcctttttc cgaataaacg gtgctcaaaa gcttgcatct gcggtaataa
     4801 gtccaaccaa gttaaaaaca taaaaagctg tttttacttg gctgttctta ttacttgatg
     4861 caaggcagct ttttcacaac ctctgataca cggtgttcca gaagtaactt cttcggaaat
     4921 aagccgaaat ttcacaaaaa tttgaaaagc aattttctta aattccttct tatttcttga
     4981 agctgcccac ttctgacaca acctcctttg tgtaaaagaa tcagtttcta ctcgatttct
     5041 tctatcgttt cctgcaaacg ctgttcatat tttttgtttc ctttatataa atcaccaatc
     5101 gtccatgcca acaggagaag gacaagaaca atgattacat aagcgatttg atcagccaat
     5161 gctagttgag ctgaggtggc ttgctcgccg aagaaacttt cgatttgacc tggtaatcct
     5221 cgtaaattca agtaagtcag actaatcaca gagaaccagc caagtagttt tacaacaaat
     5281 gaatttttga atcggctccc catctccgct tggctattgg tcatcatcag taaaggaatc
     5341 atcgagaatg gcaatgcaaa agccagaaaa acttgggatt cattcatcaa gttattcaaa
     5401 gcggtatgtt cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga
     5461 atcacagaaa gcaatcgtgt gactagtctt cttagccaaa ttgggatacg catatggatg
     5521 aagccttcca tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa
     5581 gcaagaagtg ctacagcaaa taaagtagac aagataccag attttgctac agcaatcaag
     5641 ataccattgc tcatagctga tggatcagat aaagcttcat acagaccaaa aaaggaagga
     5701 tcttggacag aaccggtttt gaatacagcc acacccatga tcaatagcag tgcattgact
     5761 agaaaagcca ttgacaactg gatattagaa tcccaagtag aaaagcgtac cgcttgtgct
     5821 acatcttctt cgtcttggtg atcgatcttc cgagtttgag aaactgctga atgaagatat
     5881 aagttatgcg gcatgactgt tgcaccaata atccctaaag ctccagtcag cggagtttcg
     5941 ccgccgatga ccggtgagtc tgagaatgtg tgactgttag ggataaatcc ggcaaacaaa
     6001 ctgtgccaat cagggttaga caagccaact tgatagacga aaacgaataa aatgacgaaa
     6061 atcaagcaga caaccaaagc ttcgatttta cgaaacccga cttttgtcag caataaaagg
     6121 acgaaaacat ctaacacagt gataaatacg gcaattacca atggaatatc aaaaagcagg
     6181 tagagggcga ttgctgcacc aatgacttct gcgatgtctg ttgccataat ggctaattca
     6241 gtcaagatcc ataaaatgac acccaatgtc ttacttgtcc gtgctcggat tgcttgggcc
     6301 aaatccattt gggtcacgat tcctaatttt gctgccatat attgtaaaag catagcaatc
     6361 agactagaca tcaaaataac tgacatcaat aaatattgga aattttgtcc accagtgatc
     6421 gaagtggacc agttacctgg atccatataa ccaactgcta ctaatgcacc tggtccagag
     6481 taagcaagaa gtgttttaaa aaaacttttt cccttgggta catccactgt tccatttact
     6541 tcagctaaag ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttgttg
     6601 gattgattat tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc
     6661 agaaagaaaa aattaaagaa aaagatttgg tatgcctaat tttttttcgt ggcgtgtctt
     6721 gctggttaat gaaagaaatt ttgttcttct ctaaaaaaac cactattttt tgcatgaaac
     6781 taaagaatac gcctttcttt tttcaataaa ctttagtaaa ataaatcatt gagagcaatt
     6841 cggaaataga aaggagagat ttatatggta tcaacaggta ttgtcgtact tattgctatt
     6901 attgctttgc taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac
     6961 tatttagaaa aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg
     7021 gggcaaaaac catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg
     7081 aaaaaatagg ttctgaccca aagacttttt gcgggctgat tttttcagtc cgttttttat
     7141 ttcgtaattt gttagactat tcagactatt cagcaaaaaa gacaagcaca gacaatccta
     7201 tgctcttgat aaagacaaag gaggagtaaa atgtcgattt ttagaaaatt aggttggttt
     7261 tttcgacagg aaaagaaaca ttatttaatt gggattttct ccctattcgc agtggccttg
     7321 gtacagttag ctcctccaaa agtgattggg atcatcattg atgagatcgc cgataaaaat
     7381 ttatcaataa agatcatttc ctggtggatc ttattattga tccttgcagc tctcttacaa
     7441 tatctttttc gttatatctg gcggacgaat atttggggaa gtgcagctcg gttggagaaa
     7501 gaacttcggc agcagctatt caaacatttt acagaaatgg acaatctctt ttaccaaaaa
     7561 tatcggactg gagatttgat ggctcatgca acgaatgatt tgaatgcaat ccaaaatgta
     7621 gcaggagctg gtatcttgac ctttgcagat tcagtgatca cgggcggaac aacgatcatt
     7681 gcgatgattt tgtttattga ttggcgctta acactgattg cgctgattcc tctgccttta
     7741 ctagccgtta cttcacgagt gctggggtca aaactccacg atgcttttcg tgattcgcaa
     7801 gaagcttttt ctagtatcaa tgataagaca caagaaagta ttactgggat caaagtcatt
     7861 aaaacatttg gacaagaaaa agaagatctc gcggattttg aagaaaagat tgaagatgca
     7921 attattaaaa acaaacgagt caatttttta gactccttat ttgatccatt cattaccttg
     7981 attatcggtc tgtcttatgt tgcaacaatt attttaggtg gaaagtatgt cttggaaggc
     8041 acgatctcaa tcgggcagat ggtctcattt attagctata tcggtatgct tgtttggccg
     8101 atgtttgcga ttggtcgatt gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc
     8161 gtcaatgaac ttttgcatga aaagacgcat atcgtggaac aactgggtgc agtgcagact
     8221 ccggcaaaag gcgagctgaa tgtagctatt tcctctttta actatccgaa agaagaatca
     8281 ccagcattag aacaaatcaa atttgcgatc caagaaggag aaacattagg tattgttggg
     8341 aaaactggtg cgggaaaaac gacaatcatc aaattactga tgcgtgaata cgatgattat
     8401 caaggaaaaa ttcaatttgg cggatgttcg atcaaagaat attcattgaa tgctttatta
     8461 ggatcaatcg gctatgtgcc tcaagatcac ttcttgtttt cgatgaccat tcgagataat
     8521 atccgttttt ctaatccaaa actttcagaa gaagaagtag agcatgcggc ttctttagca
     8581 tttatcaaca atgaaatcaa ggaattacca aaaggttatg ataccttagt tggagaacgt
     8641 ggagtctctt tatctggcgg acagaaacaa aggatttcga ttgctcgagc attgatcaaa
     8701 aatcctgaat tgctgatctt agatgatgca ctttctgcag tagatgccaa aacagaagaa
     8761 gctatcttgc ataatttaaa agaaataaga aaagagaaaa caaccatcat tgcagctcac
     8821 cgtttaagca gcgtgatgca tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt
     8881 gaacgaggaa cccatcatga gctgcttgca ttaggcggct ggtatgaacg aatgtgggag
     8941 aaacagaaat tagaagcgaa gatagaagga ggcgaggcct gatggatgaa aattatcaat
     9001 cggaatggac aaaatcgatt ccacttaaag aacagaagaa gatcatcaaa cgactgatga
     9061 agttcgccaa accttttaga agaacgttca tcgttgctat cttatttgcg tttgccctgt
     9121 ctgtgatcaa tgtcctattg ccaaggatca tccaaacttt tatggatgat catctggcta
     9181 aacagtcggc tacgacacaa gttattttgt tttttgctgg tatgtacttg tttggagtca
     9241 tagtcaaaag tatcatttgg tttttccaat ggtatttgta ttccatggct tcattgaaga
     9301 cctatcagta tgttcgggta agattatttg aaaagctgca tacactaggt atgcgttatt
     9361 ttgatcagac accggcagga tcgactgttt ctcgagtgac aaatgacaca gaaacgctat
     9421 ttgaattttg gtatgtcttt ttgatggtat tgactgggat ctttgcagtc gtttcttcct
     9481 tcattgccat gtttcaaatc aatggagaaa ttgccttgtg gactttggtg ttcttgccga
     9541 ttttaggaat cgtcatctgg tattaccaga aattcagttc tcgtatttac cggaatatgc
     9601 gggaaaaatt aagtcagctg aacacgaaat tgaatgaatc gatttccggc atgcgtatta
     9661 tccagcagtt tcgccaagaa gctcgtttag ctaaagaatt tgaacaagtg aacaacgagt
     9721 atttagacac gcgctatgcg atgatcaaaa caaattcgat tctgttaagt ccgatcatca
     9781 attttctata tgcattggcg attgcgttgg cgttgacttt gtttgggatc gatgcgctgc
     9841 attctcccgt agaagttgga ctgatctatg catttacgac ctatgtccag ggatttttta
     9901 atccaatgtc tcaaatgatg gactttttga gcacctttac agatggaatg gttgccggta
     9961 gtcgtatttt gaaaatcatg gatacagaag aatatgcacc gcaacaaaat cctggagcag
    10021 atcaagcgat tacttcaggt aagatcgagt ttcgcaatgt aagcttttct tatgatggag
    10081 agcacaatgt attgaacaat atctcttttg tcgctaatcc gggagagacc gttgcattag
    10141 tggggcacac cggaagcgga aaaagctcga tcatcaacgt attgatgcgg ttttacgaat
    10201 tttatgaagg ccagatcttg attgatgatc atgacattcg agaatatcca atggaagagt
    10261 tacgtaagaa aatgggattg gtcttgcaag atgccttctt gttttatgga gatatcgcag
    10321 gaaatatccg gatgatgaat ccaactatca cagacgaaca gattcgccat gcagctgagt
    10381 ttgttcaagc aaacaagttc atcgatgaat taccaaaagg atatcactca aaagtgatcg
    10441 aacacggtgc cagctattct agcggacaac gacagctgat cacttttgcg cgaacaatcg
    10501 tgacagatcc taaaatacta gtattggatg aagcgacagc aaatatcgat actgaaacag
    10561 aaggattgat ccaagaagga ttgacaaaaa tgcgacaagg acggacaacg attgcgattg
    10621 cccatcggct gtccacgatt cgggatgcag aattgatttt agttttagac aaaggtcata
    10681 tagtagaacg tgggaatcat gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt
    10741 acaaactgca aagcagtggt gtgtaaaaag tggttgtgac agggctccgt tcagctttag
    10801 gtgataagaa aaaaattgta aaaaaggctt tccagatttt tgcataaatt tggcttatca
    10861 ccgataagct ggtactggaa caccgtatat tagaagttgg gacagtagca tagtagcatt
    10921 ttgacctaag cgctagagtg aaaataaaac agagcttccc agattcttca tctgtgagcg
    10981 ttagtgtcgt aattaccaga tttaaaaaaa atcacgacag ctagtcagaa gtgcccaata
    11041 gccgctaaga aagcgttgac aaaagtgctt gctttcgata atataaagaa aacgatacaa
    11101 cttggatagt gattgcctga tgcaagctaa acctgatgag tctcccatgg ttccatggac
    11161 ggctttttgg gtttttcttt ttgttttgga tacagcccta tatatcatgg aaaggagtaa
    11221 aacatgctga ttgagaaagt actgaataac aacgtcgtaa taaccaggga tgaaagaaat
    11281 caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt
    11341 tttgactcat gcatcgacaa aaaatttcag ttgacaaatg acggactttc acaacagttc
    11401 caagaattgg cacaagcgat tcctcttgag tacttagaaa tttcttgtga aatcattgaa
    11461 tatacaaaag aggctctaca agtccagttg aatgattcgg tgtatattgc attgacggat
    11521 catttgtata cggctatcga acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg
    11581 tatgatatca aacaactatt tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa
    11641 atctcggata aataccacat caagttatca gaagatgaag ccggatttat cgcattgcat
    11701 ttagtcaatg cacaaggcat tcaaagccaa caagacaata cctacctgtt tactacaaca
    11761 atacaggata ttctaaatat cgtccgctat tactataaga ttacatttga tgaagattca
    11821 gtccattttt ttcgattcgt cacacaccta cgtttttttc tgacacgcgt cctagatgga
    11881 gaaaaatcag aagtgggaga agtttctgag gaattgtacc aggttattca aaaaaaatat
    11941 aaggatacgt ttggttgcgt tgagaaaata gcagactttt tagccgagat attcaactac
    12001 cagatgtcca cggacgaaaa aatgtattta acgatccata ttgcgcgttt agtgcatgag
    12061 aatcgttgta aataaataaa aaaaggagga taaattaaca agaacgcatg aaataagggt
    12121 agtgacattc agttggctaa accttccact aataaattgg agggaataga aatgggaaaa
    12181 tatcaagaat tagcgagcaa aattgtagaa aatgtcggcg ggaaggaaaa tatcaatggt
    12241 ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa agcaaatgat
    12301 gagatcttga aaaatatgga cggtgttgtg acggtaatgc gagcgggagg acagtaccaa
    12361 gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc aggaaacctt
    12421 acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct tattgatgga
    12481 atatcgggct gtttccagcc attccttgct attctagctg ctggtgggat gattcgtgga
    12541 ctgacagcat tacttgtctt cttaggcgca tttgaccgcg gttctggcac atttgttatg
    12601 tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg tttaacagct
    12661 gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggggcagc attaatgaat
    12721 cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactgac taccatattt
    12781 tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc ctggattgcg
    12841 cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc tagccaaatt
    12901 cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt tccattcttc
    12961 accgttctta ttacaatgcc cttaggtttt ttactcgttg gaccagcatt cacattcgca
    13021 actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt tatttatgga
    13081 gcaatcgtag gcttcttctg gcaaattcta gtgatgtttg gtctccattg ggcaatcgta
    13141 ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atatcatgac acctgttgca
    13201 gtagtgtctt ttggacaaac tgcagcactt actgcactct actttaagct tagaaaccca
    13261 aaagacaaag caatcgcaat accagccatt gtttctggta ttgtggggat tacagagcca
    13321 gctatttatg ggtttacatt gccacgtaaa aaaatattta tttttacctg tgtcggtggt
    13381 gcgttagggg gcgcgtatag tggattaatg aatttaactt cttggaatcc aggtggatta
    13441 ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacgga tgtgattaat
    13501 gtattgattg gaatcgccat tgcgatggtg gtatcctttg cgctgacttt tttcttttgg
    13561 aaggaggaag caggtgaaac agatgataga cagaaaagat caggtaaaga aattgtaaaa
    13621 acaccgatac aaggacaaat cgctccactg aatgcagcaa aggatgcagc ttttgcacaa
    13681 ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg tgcccctttt
    13741 gacggaacag tcatgaccct gttcccgacg aaacatgcaa tcggaatggt ttctgaagca
    13801 ggtctggaat tgctgattca tgtcggattg gatactgttc agttggaagg gaaatatttt
    13861 gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg atctgttgct ccgcttcgac
    13921 aaaaagaaaa tcgaagagga aggatacagt ttagaaacac ctgtgatcgt caccaattac
    13981 acggattatt tggatatttt ggaaaatcaa ggggagacgg tcgggccaga cgatgcactt
    14041 atcaccgcac tgacctgaac ggttaggtga aagaatggac taggcttaat tattcttttt
    14101 agtccacttc tttattttca aaattgaaag gctctttcat gatggctgag ctgatagggc
    14161 gtttatcaaa tttttgtcct acagaaatta gatggactaa attctgttga taattttctg
    14221 caaaaacaca actatataac acgtctagaa gataacaaat cgatgtattg atcgtaaaat
    14281 taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg caaagcggct gtactcattg
    14341 aagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga ttttgtttca
    14401 atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc agaatcccgc
    14461 acatatcact cgtgcaattg tacgcttcat agaacgattc accaaaagtg gtgacaatcg
    14521 tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc aaagtattcg
    14581 agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg gctttttgca
    14641 gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt catctgttct aaagaagcca
    14701 ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc gcatctacgt
    14761 cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga aaactgaccc
    14821 agccatcaaa acccattttt tttgctacac ggatgagtgt ggacgggtgg acataacatc
    14881 gctcagcaat ctcttggatg gtcatttcgc caagtgctgg agaatcgctt aagagaaact
    14941 gaatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca cgtaataaca
    15001 tgtagatacc ttcttcttgt ttttttacgt aaattataac ttttttgaaa at
//
LOCUS       DADVXO010000070        14820 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.072, whole genome shotgun sequence.
ACCESSION   DADVXO010000070 DADVXO010000000
VERSION     DADVXO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.072"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(296..1024)
                     /locus_tag="KB950_001801"
     CDS             complement(296..1024)
                     /locus_tag="KB950_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBB6858019.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="KB950_001802"
     CDS             complement(1002..2576)
                     /locus_tag="KB950_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6858020.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="KB950_001803"
     CDS             2805..3743
                     /locus_tag="KB950_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858021.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="KB950_001804"
     CDS             3755..4666
                     /locus_tag="KB950_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858022.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="KB950_001805"
     CDS             4690..6162
                     /locus_tag="KB950_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6858023.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="KB950_001806"
     CDS             6213..6824
                     /locus_tag="KB950_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6858024.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="KB950_001807"
     CDS             6854..8767
                     /locus_tag="KB950_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HBB6858025.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="KB950_001808"
     CDS             8895..11876
                     /locus_tag="KB950_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HBB6858026.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="KB950_001809"
     CDS             11873..13534
                     /locus_tag="KB950_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HBB6858027.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="KB950_001810"
     CDS             13574..14506
                     /locus_tag="KB950_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBB6858028.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2333 c         2979 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DADVXO010000071        14697 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.073, whole genome shotgun sequence.
ACCESSION   DADVXO010000071 DADVXO010000000
VERSION     DADVXO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.073"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(171..737)
                     /locus_tag="KB950_001811"
     CDS             complement(171..737)
                     /locus_tag="KB950_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6858029.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(737..1693)
                     /locus_tag="KB950_001812"
     CDS             complement(737..1693)
                     /locus_tag="KB950_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBB6858030.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1693..2349)
                     /locus_tag="KB950_001813"
     CDS             complement(1693..2349)
                     /locus_tag="KB950_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBB6858031.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2719..4737
                     /locus_tag="KB950_001814"
     CDS             2719..4737
                     /locus_tag="KB950_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6858032.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4807..5013
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5164..7578
                     /locus_tag="KB950_001815"
     CDS             5164..7578
                     /locus_tag="KB950_001815"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBB6858033.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7926..9479
                     /locus_tag="KB950_001816"
     CDS             7926..9479
                     /locus_tag="KB950_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBB6858034.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9860..11644)
                     /locus_tag="KB950_001817"
     CDS             complement(9860..11644)
                     /locus_tag="KB950_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858035.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11659..13380)
                     /locus_tag="KB950_001818"
     CDS             complement(11659..13380)
                     /locus_tag="KB950_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858036.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13480..14241
                     /locus_tag="KB950_001819"
     CDS             13480..14241
                     /locus_tag="KB950_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6858037.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4777 a         2713 c         2921 g         4286 t
ORIGIN      
        1 taagaacagc caatcaaaaa cagcttctca tgtttttgat ttggttggac ttattaccgc
       61 agatataagc ttttgagcac cgtgaataaa gaggttatga aatcaacgct ctaacctgag
      121 tattagagaa aaaaacgcaa aacatatcat ttatgttttg cgttttttat ttatcttttt
      181 ttctcaatac aatataaaat aggcggctgg ttcttttggt tgataaaccg ataattcagc
      241 acattatatg tttcctgtgg cagtttggaa caaaaagaat caaccatatc cagttctttt
      301 tctccacctt catgaccata gtagacgaca aggatcaatc gtccgcgagg cacaagtcgt
      361 ttcaatattt cttccatagc tgtgcgtgta gtctctggca tagtgatgat tgctttatca
      421 ctttttggga gataccctag attgaaaatc cctgctttga gattttgttg ttcagctatc
      481 acttctccta acgtttcgtg gccttgtaga aacaatgaga cacgttcttc taactgacgc
      541 tgttctagcc tttcacgagt agcatcgatt gcttgttgct ggatatcaaa actatagaca
      601 tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat cgtggccatt gcccatcgtt
      661 gcatctacga cgtgatcccc tggttgcaag atttcttgca gtaatgtatg gctgaaatgc
      721 aaagcagttt gcaacatcag acgctcacct ttttccgtac atcatatttt ccttggaagc
      781 tatctctgcg aagaagttct tcatcaattg cattcaagac ttcccatttt ttcaagctcc
      841 acattggtcc aatcaaggaa tcccacggcg catcccctgt caaacgatgg atgatgatct
      901 cttttggtat catttccagc tggtcgcaaa tgacattgac atagtcagga cggctcatta
      961 attgaaggcg accttcatga tagtcccgca acattctcgt tttgcgcatc agatgcatca
     1021 aatggagctt gattccttgg atatctgaat ctaaaatggt gcgtctgaca ttttctttca
     1081 tcatttctaa actttcgcct ggtaatccgt tgatcaaatg cgtacaaaca cggatattat
     1141 gtttgcgtaa tttagccacg ccatctaaat aagtttgata atcatgtgct cggttgatca
     1201 gttttgaagt ttcttcaaaa gttgtttgca atccaagttc tacccataaa tacatccgct
     1261 cgttcagttc agcaaggtaa tcaactactt catctggcag acaatccggt cgtgtaccga
     1321 ctgataatcc tacgacacct ggtaaattga ctacttgctc aaatcgttct ttgattattt
     1381 caactggcgc atgggtgttt gtaaaatttt ggaaataaac gatgtattgt tggacatgag
     1441 gccatttttg atgcatcatg tcgatttctt tctggaattg gattggaaga gggtcttcag
     1501 gtgcaacgat catgtctccc gaaccagata cactgcaaaa agtacaaccg ccatgggcaa
     1561 cagtcccatc tcgattcgga cagtcaaatc ctccatcgat cggcaccttg aaaattttcc
     1621 cgccaaactc atttcgtaaa gcgtaattcc aagaatgata tcttttattt ccttctgaat
     1681 aaggaaaagt cattaaaagt ccttctttct gtaagagtta tctatttgtt ttcttttttc
     1741 tctatacact ggataagtca ggcacagcca agaaagactg agtaagtaac cgccgatgat
     1801 atcagtagga aaatgaacgc ccagataaat gcggcttatc ccgacactta ctataataaa
     1861 catcatcagc aattgaagca atgtgcgtaa ggttcttttt tgaacaaaag ctggaagcag
     1921 aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca tgtccactag ggaaactgta
     1981 acttgtttca actactaagt gcgccaatga aggacgctct cgcatgaaaa atagtttgat
     2041 caccgggttg atgattccag caattacaat aaatccagaa acgaaccaaa atgtttctgc
     2101 cctttttttc ttataccaca tccataaagc cgcacaagca aataaaatga tgacggtaag
     2161 aggattaccg aatttagtca taaagagttg gtagctgttc agatcaggat aagaagctct
     2221 gacaatgcct gtaaaccact gatcgaaacc agaaagccac gacagatgga actttacgat
     2281 ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca gccacctgaa atgatgtttt
     2341 atatttcata caattcctcc tttgaagcaa tgacttatta tagcattttt ggtccagcga
     2401 aaaaaacggt aaagagataa aaccaatgta tgattcctct ttgaataacc aatattctta
     2461 taaaaaaaat aactgtaatc ggaaagacat tttttggtaa tgcaaatgaa atgttaggga
     2521 aataaaatca gtgtttttcg aaatcctttt aaacaagaac gtagacgtag gaagaacttg
     2581 aaaatgaaca gaagttgttc attacggtaa attacaatat gattacaaaa aacaacaaag
     2641 ctcttaaata acttttaata aatattaaga gaagttataa ttattaataa tagataaatt
     2701 gaagttggag gatagaagat ggaaaacatt gcaagaaaag aacggagacg tttagaacag
     2761 accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg tgggaactgc gatggtaggc
     2821 tgttctgtcg ctgccccatt agtacagccg gttcctgttc aagcggatga aacacctgtt
     2881 cagttcggcg caagaatcaa tacagcggca tttatcacag aaattgctac ttatgctcaa
     2941 ccaatcgctc aagccaacga tctatatgca tcagtgatga tcgcacaagc agttgtagaa
     3001 agcggttggg gcagtagtgc gttatctcaa gcaccttatt ataatctttt tgggataaaa
     3061 ggcagctatc aaggacaaac cgtctacatg gatacgcttg aatttttaaa taatcaatgg
     3121 gtaaataaaa aggagccttt ccgacaatat ccttcttttg ctgaatcttt caatgacaat
     3181 gcctatgtac ttagaaatac ttcatttggc aacggttatt actatgccgg tacttggaag
     3241 agcaacacga aatcctatac agatgcgaca gcttgtttga cagggcgtta tgcgactgat
     3301 cctggttacg caggcaaatt aaataatatc atcgcaactt acggattgac ccaatatgat
     3361 acaccagcta gcggaaacgc aggcggtgga gcgactacag gtggcaataa tactggaagt
     3421 gcaggtagta ctggcggttc aagcaattct ggtaatacaa actcaggttc agcaacttcg
     3481 accacttata ctgtccaatc tggtgattct gtttggggaa tctcaaataa attcggtatc
     3541 acaatggatc aattgatcca atggaacaac atccaaaaca atttcattta tccaggacaa
     3601 aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga cgaataattc cggtaacaat
     3661 accaattctt caggaaatgc tggtacatca aacggaggac aaacaactgg tgcgaaatac
     3721 actgttcaat ctggtgattc tgtttggaag atctcaaatg atcacggaat tacgatggct
     3781 caattgattg aatggaacaa tatcaagaac aatttcgttt atccaggaca acagctaatc
     3841 gtatcaaatg gcggttcttc taatgctgga gcaaatactg gctcaaatac tgggaataca
     3901 agtggctcta gttcgtccag ctcaagttct tctaatacag cttcaggaac gaaatataca
     3961 gttaaagccg gagactctgt ttggagcgtc tcaaataaat acggaattac gatggctcag
     4021 ctaatccaat ggaacaatat ccaaaataac tttatctacc caggacaaca actgactgtt
     4081 tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag gttcgtcaac caatacaaat
     4141 aacagcagtt cagttaatac tggcagcagt tcgtctcaag gcacttatac cgtgaaagca
     4201 ggtgaatctg tttggagcgt ggcaaataaa aacggcatca cgatggatca gttaatcgaa
     4261 tggaataata tcaaaaacaa cttcatttat ccaggacagc aattgatcgt taaaggtgga
     4321 acttcggcaa atacaaatac aggttcaact acttctgcag cgaaaccaaa tacaccgaat
     4381 acgtctgcta cgacaagtac atcatccact ggtaatacga tgtataccgt gaaagcgggc
     4441 gagtcagttt ggagcgtagc gaacaaacat catatcacga tggatcagtt aatcgaatgg
     4501 aataatatca aaaataattt catctatcca ggacagcaag tgattgtgaa gaaaggttca
     4561 tctcaatcaa caaaccaaca atcagttact ggttcgaaaa cctatactgt gaaatctgga
     4621 gaatctgttt ggagtgttgc tgacagtcat ggaattacaa tggctcaact aatcgaatgg
     4681 aacaatatta agaacaactt tatttatcca ggtcaaacgt tgattgtagc taaataaaat
     4741 agctgttcga agaaataacg attgaattcc acaaaaatat ttgttactat aaggctactt
     4801 aaatatgaca acacttgaaa aggaagtagt aaaaagactg aagtttcaga gagcgcatgg
     4861 ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg agttgccctt gctgaatacc
     4921 cgagtaagca agcggcggtg acggccgtta caacgttcga ggccttttca ctaaggtaaa
     4981 attaggtggt accgcgtaga ttacgtccta taaaaagagc aatcttttta taggattttt
     5041 ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga aataaaaata aacagattct
     5101 gccggttttt tcgttatatt tataatagaa acaagtgttt tcaatagaac aggaggacag
     5161 caaatgagtt acaatcataa agaaattgag aaaaaatggc aaaaatactg ggcaaaaacg
     5221 aatactttca acacccatga tgatcctgaa aagccaaaat tttacgcact ggatatgttt
     5281 ccctatccat ccggtcaagg gctacatgta gggcatcctg aaggttatac ggcaacggat
     5341 atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc tgcatccaat ggggtgggat
     5401 gcattcggtc tgcctgctga acaatatgcg ctagatacag gaaacgatcc agctgagttt
     5461 acccaaaaaa atatcgaaac attccgacgc caaatcaatt ctttaggatt tagttatgac
     5521 tggaatcgcg aagtgaatac aactgatccg gagtattata aatggacaca atggatcttt
     5581 acgaagcttt atgaaaaagg tctagcttat gaagcagaag tagcggtaaa ctgggtacct
     5641 gaattaggaa cagttatttc aaatgaagaa gtgatcgatg ggaaaagtga acgtggtggc
     5701 tatgatgtag tcagaaaacc aatgcgccaa tggatgctga aaatcactgc ttatgctgat
     5761 cgcttattgg atgatctaga attagttgat tggccagaaa gcattaaaga aatgcaaaga
     5821 aattggatcg gtcgttcagt aggagcaaat attgaattta aagtggctgg tacggataaa
     5881 tcctataccg tatttacgac acgaccagat actctattcg gtgcaactta ctcggttctt
     5941 gcacctgaac tagatcttgt tagagaaatt acgacccctg aacaaaaagc tgctgtagaa
     6001 gcttatatcg aagagacagc taaaaaatca gatctaaaga gaacggatct tgcaaaagaa
     6061 aaaacaggag tatttactgg tgcttatgca atcaatccag ttaatggcaa ggaaatccca
     6121 atctggatcg ccgattatgt attatcttca tacggcacag gagccatcat ggctgttcca
     6181 gcacatgatg aacgtgactt tgaattcgct caaactttcg gactagaaat tcttccagtt
     6241 atcgaaggtg gagatgttga aaaagctgct tacacagaag atggtacgca catcaactct
     6301 gaatttctag acggcatgaa caaacaagaa gcaatcgata aaatgaatga atggctagaa
     6361 gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac gtgactggct attctcccgt
     6421 caacgttact ggggtgaacc aatcccaatc atccattggg aggacggaac agtcacagct
     6481 gtcccagaag aagagctacc attacggttg cctaagacaa aaaacatcaa gccaagcggt
     6541 actggagaat ctccattagc aaatatcgag gagtgggtca atgtcgttga tcctgtaaca
     6601 ggtaaaaaag gccgtcgcga aacgaataca atgccacaat gggcaggaag ctcatggtat
     6661 tacctgcgtt atatcgaccc tcataacaaa aaagaactag cagattatga aaagttgaag
     6721 cgttggctac cagttgacat ctacattggt ggagcagaac atgcggtact tcatttacta
     6781 tatgcacgat tctggcataa attcttatac gatatcggtg ttgttcctac aaaagaacca
     6841 ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa acaacgaaaa aatgtcaaaa
     6901 tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa cttacggtgc agatacactg
     6961 cgcatgtacg aaatgttcat ggggccatta gatgcttcga ttgcttggag cgaaaatggc
     7021 ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt tgatcgttga tgagaacaat
     7081 aaaatgcgtg accgtatcac tacattgaat gatggcaagc ttgataaagt gtatcatcaa
     7141 acagtgaaaa aagtgacaga agactatgaa aatcttcatt tcaatacagc aatctcccaa
     7201 ttgatggtct ttatcaatga agcctataaa gtggacgctc tgccttatga atatatcgaa
     7261 ggatttgttc aactactcgc accgattgct cctcacatgg gtgaagaact atgggcaatc
     7321 cttggaaatg atggtggtat ttcttacgca ccatggccaa catacgatga agcagcactt
     7381 gttgaagacg aagtagaagt tgttttccaa gtaaacggta aagttcgtgc gaaaagcaat
     7441 gttccccgag acttaggaaa agatgagcta gaaaaagcag cattagccaa tgaaatagtg
     7501 caagaatata ttgaaggcaa aacagtcaga aaagttattg cggtaccaaa caagttagtc
     7561 aatatcgttg ctaattaatt ggctctttgt caataaggac tgatgagttg tgcaaaatca
     7621 aatctgggcc agaatgaact tcattctgtc tcagattttt tgttatttca aattgattaa
     7681 ttgattgatc aaaaaggttt tgattttcat gttctcaaac acgttcaatg tctttttctt
     7741 ctgaatggtc ttgtaagaga tctctgtaat catcaaattt ttgtctatta atcttagtat
     7801 tttttgatgt ataaactacc tgaaattgag agtacatacc aaattcataa attttttatt
     7861 agtgggacta gtgattgtat ggattttgac gcataatgaa tgaagtatga gaaaaggaga
     7921 gacaaatgga tcaagaacta tttattatcg aagtattatc agctaaacaa aaaattatcg
     7981 agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct tttaagctta gtatttttct
     8041 ctggtttgct ttttttcgga gctgtatcgt tcctatatcg ccatatgagt gcagtagtcc
     8101 gttcagctga agggatgcaa aaattaaata atttgtatca cttttcgtgg ggagttggcg
     8161 gaataacaat tgttgtgctt attgtttttc ttctacttat ttggctccgt taccatcaat
     8221 tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca acagttttta cataataaac
     8281 caacttctca agatgatgac tatctttatt ttgagaagac ttcgacgagt caagcttttc
     8341 ggatacaaaa aagtgattgc cagttattgc tgagatcact tgatgattta cctatctact
     8401 taggcgaaag caaatcaaaa ttcggaatga acacattcca tattttttta ctagaaaacg
     8461 aacccagatg ggaaaaaagc cagtcttcgc ctggtggata cctgacaact aagatgagta
     8521 cattagtcac tggttttttc ctcattcttt tagctggaac aatctattat gggagataca
     8581 ctcagccaaa aatgtcttct catataaatg ctgcttctca catgacaatg aactcaacta
     8641 acgatacgca tatacaaaag acaaatgaag ggttgttagc tgatcctaag caaaaggtaa
     8701 atttgacaac acaaaagata aatgatctca aacttgatac agagacgcac tctctctata
     8761 tgacaacaga tagtggtgct aattgggtat ttgtgccgat tgattcgcaa tggttgcgtt
     8821 ttggcgaata caccctgaca acggggctga ttcctgaagg ttattggatg gacaacacat
     8881 atgatatttc cgccgatttc tcatggttta tctattcttc tggggatgat gtacgtctgc
     8941 tgacttcaag agataatggt aaaacatggc aaacgtctat tatcacccaa tcagttcagc
     9001 cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg tctatttatc tgtacatcag
     9061 ccagtgggat gtctgcagaa actttgtaca tttacactac tatggatggc ggaatcactt
     9121 ggataaaaag tggaagtaca atgatcaatc agccagtaca aaatgtcagc ttcgttacaa
     9181 cttctttagg ttttgtttcg acacgtgaaa gtttgtttta tacaaacaac ggaggacgct
     9241 cattcaaaga atcagttctg tcgattccgg aaaactatca gacagggggg atagatcttt
     9301 ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac gttagagaca aaattttatt
     9361 tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc ctgcctcttc cgttcaactg
     9421 ataacgggga aacatggcaa ttcgttcagc aactatcgca agtagaacaa ggagattaaa
     9481 gaagagagat tgtgagcctg acgttcagca ccgagtcaca agaaacaaat tcaaaaaaca
     9541 gcttctcata tttgttgaat agtgtaactc gtgattcgga gttgacaaga gcaacaagaa
     9601 gcgattttga agtatagctt ctcgtatttt acaagatcgt gtcttattgc cgataggttg
     9661 tctcttgaac accgtttatt cgtaaaaagg gattgtgaca agtcttttgt cactacttac
     9721 cgaacgaaac tgaaggagct atccaaaaag tatcattagt attattcttt gtgtttctag
     9781 tggcaccact agtacattta aaaaagctat cgatcatgga ctaatcatac atgatcgata
     9841 gctttttaga taatgtttgt tacgctatct gaaattgact attataaaga ctggcataaa
     9901 gcgttggtga ttggagtagt tcagaatgtg ttcctttttc gacgatatcg ccattcttca
     9961 ttacaaggat catgtctgca ttttcaatcg ttgacaaacg gtgtgcaatc acaaaacttg
    10021 tcctatcttt tgtcacagct tccattgctt gctgaatcaa tgcttctgtt cgtgtatcta
    10081 cgctagaagt tgcttcatct aaaataacga caggaggatt cgctagaatg actcgagcaa
    10141 ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt ttcgctagaa atgatcgtat
    10201 catatccgtc tggcagtgtt cgaataaagt gatcacattg tgcgatttca gccgcttcga
    10261 taatttgttc tcttgtggca tccattttcc cgtaggcgat attatcagct accgttcctt
    10321 caaacagcca ggtattttgt aaaaccatcc caaaagaacg acgtagttct ccacgtgaaa
    10381 gcttggtaat atcaagaccg tcgaattcga tctttccttt attcagttca taaaaacgca
    10441 tcagcagatt gactaaagtc gtttttcctg caccagttgg accaacgatc gctaccgttt
    10501 gttttggctt tactgaaaaa tcgacttgat tcatcaatat tttatctgat gtgtaaccaa
    10561 actgtacatt tttgaaagct atttttcctt tcggttgttc taaaagtctt gcatctggaa
    10621 ggtctggaat ttcatctgtt tcatctaata tctcaaatat cctatcgatt gctgccattg
    10681 cggattggat cgagttgatc acataagaag cggtagaaat tggttcagaa atctgattga
    10741 tgtactgaag ataagcttgt aagaagccga tcgtcacacc acctgataga acaagcatag
    10801 caccgaatac cgcacttatg atgaaggcga gttgattaat gaaacgaatg gccggataga
    10861 tagcacagtt cataaattga gcacgtttaa aagcgttgta ttgcttttta tttacttcgt
    10921 gtatagcagc ttctgcatga gcttgctgat tgaatgtttt gaccactaga ttaccagcta
    10981 agatttcttc cattcgatta tttaaaacac ccagttcata ctgattctgt tcagaaatct
    11041 gtttgttttt gttcgcaatc ttagttgtaa caattgaact gccaacgatc agcaataaga
    11101 cgagcaaagt aagcttgctt tcgatataaa acagcatcag accagcagca gctattgtta
    11161 cgacagaagt aaaaaattga ttgatacctg tcaataacac ttgagaaagt tgattgagac
    11221 cggtagttgt ccgactaatg atgtcaccta cttgatgatt gtcaaaaaac gacatcggta
    11281 atgttttgaa tttttttgtg atttcttttc gcaccctcaa tgtgatcttt tcgcttaacg
    11341 ctgccattgt ccgttcctga ataaatgaag tgatgctgct taagactgaa cagccgatca
    11401 gcaataagac agggaacaac aatgcttgtt tgatatcact gaaagaaagc tgtccaatac
    11461 ctgtttttct tatttctgat aagagatcat cgatcccgat tcccatgatc aagggcatgg
    11521 ctacgactaa taggttcccg aataaactgc atagaaacaa tccgtaaaaa tatttatgtt
    11581 caggtttgat cattttccaa aaacgtatca aagcacgcgt gttcaacgtt tttttctttt
    11641 ccatcgtatc ccttctttct atattccttg tgattttgca aactcctgat aagtttggca
    11701 attagacagt agttcttcat gagtaccttg tccaacgatt tttccttcat ctaaaacaat
    11761 gatgttatct gcttgttgga ttgtactgag gcgttgagca acaatcacga gagttttctc
    11821 cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct gttttatagt ctaaagcgga
    11881 gaaactatca tcgaatacat agatctctgc tggtttgatc aacgctcgag caatacatat
    11941 ccgctgtttc tggccccctg aataattaga accgccttga gcaacaaagc tgtccaatcc
    12001 gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc ttgatcgctt tagacaactc
    12061 ttctgttgag gcatattcat ttcccatctg caagttgctt aaaattgtcc cgctgaataa
    12121 gaaagccttc tgagggacat aactgatgtg ggagcggatc tgttcttgag tcatttgagt
    12181 aagaggaata ccaccaaggg ttacttttcc tatcgtaggg tcactaagtc tcaagagaag
    12241 tttcgccaaa ctactttttc cagatccggt cccgccaacg atcgctgttg tttttccttt
    12301 aggtattgtg aaatcaatat cttctagaac tggatcagag atttcagtat atttaaatgt
    12361 gacatgttca aaagcagctg catttgtctg atcaaaggtt ggttcagcta ctgttttgat
    12421 cgtctcttcc tcaatttcga tctctgcttg cagtatttct tctatccgtc gtaatgaggc
    12481 aaacgattta gggataatga ccaataccat agctgcgatc atcaagtaag caagtgtcag
    12541 catagagtat tcgactacag cagtcacgac accgatttgt aatgctcctt ctgctacaag
    12601 atttccgcca aaccacaaaa tagaagcata aacgattccc atgagcaaaa aagcaattgg
    12661 agtgacccaa gcaaatattc ggttcacttt gatcatttga ttagcatagt cagcaaagct
    12721 cttattcgtt cgcttttctt catatgattg attatcaaag gcacggatca tattaatccc
    12781 agtaaagaat tctctcaatg tgatggtgat ccgatccatt tttggctgga tactcaaaga
    12841 taaaggtgcc cctcgtttca tcagtcgata aacaatccat acataaaaaa caacagagat
    12901 caatgtaacc catgttagta atagagaata acgaaacatc atgagaatgg tgaaaacgca
    12961 aataaccggt gctggtaaga tcatttggca aaataaaaca atcatttgct ggacattatc
    13021 gacatcattc gtcattcttg tcaaaagaga tccagaacta aaacgactga tatccttcat
    13081 tgacagctgt tggaatttac ggaaatattt ctcccgcgtt tggaatccaa acctagcgga
    13141 agccaatgct gaaaagtaac tagaggcaac tgcagcgatt gttcctagaa acgctacaag
    13201 cagcatttca actccaattt tttttataac tgaggcttcg ccacttgcta tacctgtgtc
    13261 gatcagcttt gctactaatt ttggtacacc taatgtaccg gcgatttcta aacagatcat
    13321 cacaaaggta aggagagcga gtcctttgtt tttctgtaag aatgttttta agatagacat
    13381 attattcctc cttttttcct gatacttttc gattctatac cttcaagtaa cttgaaggtc
    13441 aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa tgaaaaaaat gtatacgatt
    13501 ggccaagtat cggaactatt tgatttgcca aaatcaacga tccgctattg ggatgaacaa
    13561 ggattaatcc gttcaagtag acaagaagag aatgattatc gattgtttga tatcgatgat
    13621 atttttatgc tttatgatat cgttttttat agaaaattag atataccgat caaacagatg
    13681 aagaatttat atggaaaaac attgacggag ttatatgaga cacttgatga aacagaaaga
    13741 agaatccata aagaactggt ggtgatgaaa caaaaacaaa aagagataag ggagcaaaaa
    13801 aaacagttga aattgatgat cgatacaaac gaggaagaat ttcctgtaga agagatccct
    13861 tttgactgta tgatctcaac ggagtttgaa gatattgtgg aaatcaaaaa atttctacct
    13921 aactattcaa gctttgggat gatgagtatg ccagcttcca gttcacagac gatgtatgga
    13981 ttttttatcg accctagtga agtacattta tttactcaag acgtgatttg ggagaaaaaa
    14041 gatacggctg tttatcgtcg attcttattg aaatccgaga tgaaccatgc ggaacgtaat
    14101 aatattttag aaatcagaga aagaatgtat gaaaaaggat ggaaaacagg agaagtgatc
    14161 ggccagtatc tattgacgaa tacggatgaa aacaatatca gaactgaata ttatcatgca
    14221 tggatagaaa tgaaaaaata ggaaatatgt gtttaagata actcgaaaat gatggttgtt
    14281 ttagttataa agaaaaattt tctggttcga ttacgggttt aatgtggggg attattagtc
    14341 gtggatagat agaattttag ttttttagag gaaatatgtc tttaaaaatg gaatggttgc
    14401 aattcttagc gatatatacg atgtaattgt cctcttaaaa aagtatggaa aaataattgt
    14461 attgggtgtt atgtataatt catggtgaac tgagactaat gagaagaaaa attcatattg
    14521 ttatgagtga gattgtatca gatgagaatg ataaattaaa taggtgcata aaagcattac
    14581 acttaaaata cattgttgct tacattatta caatatgtta ataaggcaaa aataaataga
    14641 tggattgaat aaacatattt tttaatcaaa tagtccctac tgtgactttc ctcagct
//
LOCUS       DADVXO010000072        14438 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.074, whole genome shotgun sequence.
ACCESSION   DADVXO010000072 DADVXO010000000
VERSION     DADVXO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.074"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(228..1115)
                     /locus_tag="KB950_001820"
     CDS             complement(228..1115)
                     /locus_tag="KB950_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858038.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="KB950_001821"
     CDS             complement(1112..1981)
                     /locus_tag="KB950_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858039.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="KB950_001822"
     CDS             complement(2118..3404)
                     /locus_tag="KB950_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6858040.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="KB950_001823"
     CDS             3677..4255
                     /locus_tag="KB950_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6858041.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="KB950_001824"
     CDS             4255..5184
                     /locus_tag="KB950_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6858042.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="KB950_001825"
     CDS             5181..6917
                     /locus_tag="KB950_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6858043.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="KB950_001826"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="KB950_001826"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBB6858044.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="KB950_001827"
     CDS             complement(7813..8322)
                     /locus_tag="KB950_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBB6858045.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="KB950_001828"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="KB950_001828"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBB6858046.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="KB950_001829"
     CDS             complement(9623..10714)
                     /locus_tag="KB950_001829"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBB6858047.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="KB950_001830"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="KB950_001830"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBB6858048.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="KB950_001831"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="KB950_001831"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBB6858049.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="KB950_001832"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="KB950_001832"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBB6858050.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4112 a         3144 c         2609 g         4573 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactga
//
LOCUS       DADVXO010000073        14304 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.075, whole genome shotgun sequence.
ACCESSION   DADVXO010000073 DADVXO010000000
VERSION     DADVXO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.075"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            48..1787
                     /gene="yfcC"
                     /locus_tag="KB950_001833"
     CDS             48..1787
                     /gene="yfcC"
                     /locus_tag="KB950_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBB6858051.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1944..3128
                     /gene="iadA"
                     /locus_tag="KB950_001834"
     CDS             1944..3128
                     /gene="iadA"
                     /locus_tag="KB950_001834"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBB6858052.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3239..3442
                     /locus_tag="KB950_001835"
     CDS             3239..3442
                     /locus_tag="KB950_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBB6858053.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3543..4205)
                     /locus_tag="KB950_001836"
     CDS             complement(3543..4205)
                     /locus_tag="KB950_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858054.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4218..5720)
                     /locus_tag="KB950_001837"
     CDS             complement(4218..5720)
                     /locus_tag="KB950_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858055.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6268..7947
                     /locus_tag="KB950_001838"
     CDS             6268..7947
                     /locus_tag="KB950_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBB6858056.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8074..8553
                     /locus_tag="KB950_001839"
     CDS             8074..8553
                     /locus_tag="KB950_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBB6858057.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8772..9452
                     /locus_tag="KB950_001840"
     CDS             8772..9452
                     /locus_tag="KB950_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBB6858058.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9460..10791
                     /locus_tag="KB950_001841"
     CDS             9460..10791
                     /locus_tag="KB950_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6858059.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10806..11768
                     /locus_tag="KB950_001842"
     CDS             10806..11768
                     /locus_tag="KB950_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBB6858060.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11879..12217
                     /locus_tag="KB950_001843"
     CDS             11879..12217
                     /locus_tag="KB950_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBB6858061.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12296..13003
                     /locus_tag="KB950_001844"
     CDS             12296..13003
                     /locus_tag="KB950_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBB6858062.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13106..>14304)
                     /locus_tag="KB950_001845"
     CDS             complement(13106..>14304)
                     /locus_tag="KB950_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858063.1"
                     /translation="NYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKF
                     HLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEK
                     ISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPC
                     EETETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPL
                     NHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKID
                     TIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRK
                     DAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYE
                     NKTKRM"
BASE COUNT         4549 a         2485 c         3099 g         4171 t
ORIGIN      
        1 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
       61 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      121 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      181 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      241 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      301 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      361 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      421 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      481 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      541 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      601 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      661 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      721 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      781 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      841 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
      901 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
      961 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1021 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1081 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1141 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1201 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1261 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1321 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1381 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1441 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1501 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1561 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1621 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1681 ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt
     1741 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1801 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1861 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     1921 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     1981 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2041 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2101 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2161 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2221 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2281 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2341 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2401 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2461 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2521 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2581 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2641 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2701 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2761 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2821 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     2881 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     2941 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3001 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3061 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3121 gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat
     3181 ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat
     3241 ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga
     3301 agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact
     3361 aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc
     3421 tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa
     3481 tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt
     3541 ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt
     3601 tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt
     3661 tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca
     3721 tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc
     3781 tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga
     3841 gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt
     3901 gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata
     3961 atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga
     4021 tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt
     4081 tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct
     4141 acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt
     4201 aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct
     4261 atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc
     4321 aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga
     4381 ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc
     4441 atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt
     4501 agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca
     4561 agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct
     4621 cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc
     4681 agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt
     4741 gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta
     4801 aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga
     4861 tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca
     4921 tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata
     4981 cccatactgc cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg
     5041 gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt
     5101 tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct
     5161 tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac
     5221 acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca
     5281 ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg
     5341 gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata actctttaca
     5401 ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg
     5461 cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta
     5521 aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga
     5581 ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg
     5641 gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat
     5701 gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca
     5761 acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg
     5821 tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt
     5881 tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag
     5941 ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt
     6001 tgtataataa tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca
     6061 aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac
     6121 gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca
     6181 tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact
     6241 tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc
     6301 gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta
     6361 gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat
     6421 tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat
     6481 gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg
     6541 acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga
     6601 tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg
     6661 agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa
     6721 gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat
     6781 caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt
     6841 gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa
     6901 gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat
     6961 ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg
     7021 actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta
     7081 ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg
     7141 atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac
     7201 ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca
     7261 agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga
     7321 acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg
     7381 caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt
     7441 gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat
     7501 attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact
     7561 acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt
     7621 ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt
     7681 cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta
     7741 aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa
     7801 catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta
     7861 ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa
     7921 gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt
     7981 gctcgtcttg ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt
     8041 tataataaac gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt
     8101 tatttagaag aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa
     8161 tctttcacag aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc
     8221 gtagtgaaag gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta
     8281 gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat
     8341 tttgtattaa tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc
     8401 gcacatcctg attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt
     8461 gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc
     8521 gtccatccta tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc
     8581 agagacctca aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt
     8641 acaattgctt tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt
     8701 ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg
     8761 aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa
     8821 atggccaacg cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg
     8881 cagaagaagt aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag
     8941 atatgattcc tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg
     9001 attatgcgaa cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt
     9061 ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg
     9121 gtatcgttca gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga
     9181 tttatcatgc aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg
     9241 atattgatgt cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag
     9301 caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc
     9361 ctgtcattga acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa
     9421 taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg
     9481 attttagaat atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca
     9541 aaagaattga atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc
     9601 ggactagttt caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa
     9661 catattgaac gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc
     9721 ggggcggcag gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat
     9781 gccatggaac gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc
     9841 caaaaattag cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact
     9901 gctgagattg caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat
     9961 acatttacag tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat
    10021 atcatgcttg tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca
    10081 gatacgattt ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc
    10141 gtcaaccgcc atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg
    10201 ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg
    10261 agtattgcct ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt
    10321 tccgatgact tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag
    10381 cttgtccagc gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc
    10441 catacgattg atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta
    10501 gtagcaccac aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc
    10561 atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa
    10621 caagtgaacc tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca
    10681 cgtgtattgg aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac
    10741 accatcgttt ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga
    10801 gtgaaatgac agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta
    10861 tccacaggca tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac
    10921 tattacggga aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc
    10981 ttcgcttttt agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca
    11041 tcgtgactga tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa
    11101 aactgatcaa aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa
    11161 atacaaaagc aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat
    11221 ctttaaagat gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag
    11281 ggcgcttttt gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa
    11341 attatccatt tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg
    11401 ctaaactttc cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa
    11461 taatccttcc gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga
    11521 tcgtttcact tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc
    11581 cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta
    11641 aacgccatca tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag
    11701 aagtagagga aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa
    11761 cagaatgata cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt
    11821 caagatcttc tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat
    11881 gacattacca aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt
    11941 gatttgccca gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga
    12001 cggattagtg gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt
    12061 gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa
    12121 gaacatccgt ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc
    12181 gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca
    12241 ttcttttcct actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta
    12301 tggttatgga atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt
    12361 tatttccgga attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag
    12421 aagcaaaaac aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat
    12481 aaataatgtc ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa
    12541 taagatgttg agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt
    12601 agcagctcat gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat
    12661 tcgtgcagca cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat
    12721 ggtaggtgta ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc
    12781 tttagcatta cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc
    12841 aatccaaatt ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca
    12901 cgtattattt gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct
    12961 gttgcgcttg atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa
    13021 atcagcgctt tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga
    13081 ataagaggat gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt
    13141 tcatatacag ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg
    13201 tatccagata gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca
    13261 aatcagcatg ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca
    13321 aatggctttg aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta
    13381 actgctcttt cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga
    13441 tttggtttct ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt
    13501 cctgtataaa agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac
    13561 tactgctgga ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat
    13621 aaattggtat tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca
    13681 attgaccgac ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct
    13741 gtttttgtcg aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg
    13801 ctctctctgt ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg
    13861 aataatcatg tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg
    13921 gaaaatatgc ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt
    13981 tttcttgttt tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta
    14041 attcaagtga ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa
    14101 gatagagacg gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga
    14161 tccgtaaatg gaattttatt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg
    14221 agggcgatat ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat
    14281 tgattagtaa catcaaatag ttaa
//
LOCUS       DADVXO010000074        14265 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.076, whole genome shotgun sequence.
ACCESSION   DADVXO010000074 DADVXO010000000
VERSION     DADVXO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.076"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(218..1387)
                     /locus_tag="KB950_001846"
     CDS             complement(218..1387)
                     /locus_tag="KB950_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBB6858064.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1591..2766
                     /gene="mltG"
                     /locus_tag="KB950_001847"
     CDS             1591..2766
                     /gene="mltG"
                     /locus_tag="KB950_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBB6858065.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2902..3381
                     /gene="greA"
                     /locus_tag="KB950_001848"
     CDS             2902..3381
                     /gene="greA"
                     /locus_tag="KB950_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBB6858066.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3550..4281
                     /locus_tag="KB950_001849"
     CDS             3550..4281
                     /locus_tag="KB950_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBB6858067.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4278..5345
                     /locus_tag="KB950_001850"
     CDS             4278..5345
                     /locus_tag="KB950_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6858068.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5352..5984
                     /locus_tag="KB950_001851"
     CDS             5352..5984
                     /locus_tag="KB950_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858069.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6124..6789
                     /locus_tag="KB950_001852"
     CDS             6124..6789
                     /locus_tag="KB950_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBB6858070.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6817..7110
                     /locus_tag="KB950_001853"
     CDS             6817..7110
                     /locus_tag="KB950_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBB6858071.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(7178..8125)
                     /locus_tag="KB950_001854"
     CDS             complement(7178..8125)
                     /locus_tag="KB950_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBB6858072.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8303..9106)
                     /locus_tag="KB950_001855"
     CDS             complement(8303..9106)
                     /locus_tag="KB950_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBB6858073.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9252..10166
                     /locus_tag="KB950_001856"
     CDS             9252..10166
                     /locus_tag="KB950_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6858074.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10214..10684
                     /locus_tag="KB950_001857"
     CDS             10214..10684
                     /locus_tag="KB950_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBB6858075.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10769..11377
                     /locus_tag="KB950_001858"
     CDS             10769..11377
                     /locus_tag="KB950_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBB6858076.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11511..11711
                     /locus_tag="KB950_001859"
     CDS             11511..11711
                     /locus_tag="KB950_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6858077.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12465..13691
                     /locus_tag="KB950_001860"
     CDS             12465..13691
                     /locus_tag="KB950_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6858078.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13799..14263)
                     /locus_tag="KB950_001861"
     CDS             complement(13799..14263)
                     /locus_tag="KB950_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBB6858079.1"
                     /translation="MIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAPPS
                     WIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRNIF
                     IAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         4871 a         2190 c         2867 g         4337 t
ORIGIN      
        1 aggccctttg tcaaataatg ttggtaaaga gaaatcttag aataatttgg acagaacaga
       61 aagaagaaca cctctttctg ttctgtttat ttggaattaa agtctgtagc aaaaattgat
      121 ttttagattt gtcccacact ctttcctacg tttagtcgag tacttttttt aagcaaatgc
      181 tattcaaaga agtagtattt gctatctttt catcatatca gctatttgcc aacttagtag
      241 ttccttccat caggtcatag atttggtttc ttagatcagc agtaggcagt ttgatagttt
      301 tgtcagcttt tatattgctt tccatgatga tagcatcctg tgaatctttg ctaaatatag
      361 cagatgtatc aaaagagtcg atatttcctt gcgacaggat ataattatct ccgctatata
      421 ccccaccact atatggatgt ccattcgcat tttcacgcca taatgagaag agtaagtctt
      481 tggaaactag ttttccagat aaaacttgtg taaacagcgt atttaaatca tagacggaca
      541 tactgatatt accagtccct aattcgttat ggatctctgt ttcagattct gtcagtggtt
      601 taaagtaatc atcctcttta gacatttgat aggatagtgc atgattggat gaatttttga
      661 cttggtcata aaattttgta tgctgtaatc ctaaaggttc gatgatatca tcggtcaata
      721 atttttcata cggttgattt gttaatttga tcaaaattcc agtcaatatg gtaaagttca
      781 atgaatcata atgagaggtc tgataatctg taaattttaa gtgctgaatc gcaaattgaa
      841 cgacttcttc atcagtcatc ggtatggttg gagaagcagt tcggtgcaag cctgagcgca
      901 tatacagcaa gtcacggata gtaatttgct ggctgccagc aatttgtgga tagaattgag
      961 ccagcttagt atctaaagaa aggtgcccac gctcgacttg ttttaaaatc aaaagagtag
     1021 tcaatgcttt ttgaaccgaa ccaatctgat aataaagaga aggatcattt ggttgttgcg
     1081 tgagtttatt cgcatagcca tagccttttt ccatgatgat gcgattattt ttctttacgt
     1141 aaattgagcc aatataatca ttttttctta atagttgatc gattttttca acgagctgtt
     1201 gttcattggt agtcagtatc aacttttttt gagctgattg agcatgggca actatctgag
     1261 agtgttcatt attgtaaaat tgccatccaa gaatagttcc tgtagagaac gcactaaggc
     1321 taaacaacac gacaacgatc attaaaaatg aatattttct ttttttagca tgttttcctt
     1381 ttttcataat agtttattta gtctcctttc ttttttacag aaagaataaa gctaaaatag
     1441 cttgtatctt gataacattt attttaatct tatagtaatg gatttactac ttacattttt
     1501 gatagaatat ttaaataaaa tcattcacgc caattcaaat atcatgtata atgaataaag
     1561 ttacgaactt gatagtaagg aggagttgtt ttgtcaggaa acaaacaaca cccaagtaaa
     1621 gataaaaaag aagccagtaa ggcagctaca gcagatataa tcgacaagcg gagaaatctg
     1681 cgtaaaaaag aagataagat tgtgaggaag atcattttag tgattgctct tacattattg
     1741 attattggcg gatttcttgg atttaccgtg tatcgttatg tagatagcgg gttgaagcct
     1801 ttagataaat cagatgacca attagtccaa gtcgagatcc cgagtggctc ttctaacaag
     1861 cagattggtg agattttgga gaaggacaat attattaaaa gcgggattgt tttcaattat
     1921 tatacaaaat ttaaaaattt aactggcttt caagctggct attatcagtt agcgcctaat
     1981 atgactcttg acgagattgg caaacagttg caagaaggcg gtacttctga gccaacaaaa
     2041 gttgcagatg ggaaaattgc cattccagaa gggtacgata tcgatcagat tgctgaacgg
     2101 gtagcgaagg taactggtaa agacaagaaa gaatttttag atttagtgaa tgatgagact
     2161 ttctttaacg agttgcacca aaaattccca gaactgttgg aaagtgcatc taaagctgag
     2221 aacacaaaat accgtttgga aggttactta ttcccagcca catatgatta ttacacgagt
     2281 acttcgctta aggatctagt gattgaaatg gtgaacaaga cgaatacggt catgcaaaac
     2341 tattattcag ctatcaagca gaaaaatttg actgtacaag aagttttaac tctagcttct
     2401 ctagttgaaa aagaaggcgt aaaagaaaat gatcgtaaaa acattgcaca agtcttcttt
     2461 aaccgaatca aagcaaatat gccacttcag tcagatatct ctgttttata cgcattaggt
     2521 gaacacaaag aattagtgac gtatgaagat acagctgtag attcaccata taatttgtat
     2581 accaataccg gctatggacc tggacctttt gacaatccaa gcgaagaggc aatcaaggca
     2641 gtattagaac cagcagaaaa tgactattat tatttcgtag cagatacgag cactggaaat
     2701 gtttatttcg ctaaaactta tgaagaacat atggaattag tggaaaaata tgtaaataat
     2761 tcttagaaaa acaggctcta gacattgatc atttttagaa aaacatttac atttgaagaa
     2821 aagcgtggta atcttttttg gactatattc ctcaaaagat taccttttgc ggtattttat
     2881 cgaaaaaagg agacgttaca catggtagag aaagtttatc caatgactct agaaggtaaa
     2941 gaaaaattag aaaaagaatt agaagaacta aaaacagtca aacgcggaga aatcattgaa
     3001 cgaatcaaaa ttgcccgcgg tttcggagat ctttccgaaa actctgaata tgaatcagcg
     3061 aaagatgaac aagcatttgt tgaaggaaga atcacaacaa ttgaaaatat gattcgtttt
     3121 gctcaaatca tcgataacga cggtgtagat gcagatgagg tatcaattgg taaaacagtt
     3181 acattcatcg aactaccaga tggagaagaa gaagagtaca cgattgtcgg aagcgctgaa
     3241 gcagatccgt tttctggcaa aatttccaat gattctccta ttgcgcaagc attgattggc
     3301 aaacgtaaaa atgatgaagt aactattgct actccaggtg gcgatatgtt agttaaaatc
     3361 gtcaaagtag aacaagcgta gtctgactga aagatctaga gagttcatct tactgatgat
     3421 acactctaga tctttttctc tttgtgtatt ttagatttca acaaacgatc ttacatctaa
     3481 agtcctatag ccaacagtta aaaaaatagg tatgatgaaa tgagaatgtg tcaggaggga
     3541 gaaagagcga tgaatagttc ttggcgtttt tttgtggtag ttgaagcact actgctgtta
     3601 tttgctgttt ggcaaatcgt aaacaatact ggacttttgc ttttagtgat attcggtatt
     3661 ttcaatatat atctagctat gcgcaaacat cctcgtacta aatttcaaaa ttttcaattg
     3721 atattaggaa gtttggtcat tttttttagc ttagtcaaca gtcctgcttt atggatgatg
     3781 gctgtatttg ccgttttatt tattggtctg aaaggtgtag agatttcagg gatagattta
     3841 accaaaaatg ctttttggcg aaagaaacag atcatgatga tccaaacaga gcaattaaaa
     3901 acacataata atgagcggaa aagacagcaa ttattcggta accaacgtat tggaaacgac
     3961 gtctatgaat gggacgatat caacatcgcc gtcatttctg gtgatactat catagatttg
     4021 ggcaatacgc ttttgccaaa agatgataat attgtgattg taagaaaagg gataggcaga
     4081 acacggatct tagtaccttt gggcgtagcc atccgattag agcatgcgac attagtagga
     4141 aatgtcttgt ttgaagaaga gcaattttct ttgaaaaatg aacaaatcaa aatctacagc
     4201 agtgattatg atgaaaaccc tcgtagattg aagattatta ctaatacctt gattggagat
     4261 gtcgaggtga tacgaatatg atgggaaaaa tatccagagc gatgctagct gtttattcgg
     4321 ggattgcggc tttccttatt attctatttt cacttttcac ttatttttat gccagcaatc
     4381 aaagtcattg gtggggagaa ttactgcgtg cacgtttatt atatgttccg cttatcttcc
     4441 atttgttagc catttcctta ggtgtaggat taattgtctt tctattatta tcacttattc
     4501 aaaagacaaa atacgggaaa atcgaagaaa agctgcgtgc actttcttct ggcaattatg
     4561 aatccaaatt gttgcttctt ccgatcccaa gtgcatcgga cgatttatac atcaaagata
     4621 ttgataagga aatcactaag ataaaagaaa aaatgattga agtatctagt gaattacaaa
     4681 ttgtaacgag ccgtccccaa tatgtagatg gacaaaccaa agaagaaatt ttagaattag
     4741 aaagacatcg attagcccgt gagctgcatg attcagtttc gcaacaattg tttgcagcaa
     4801 tgatgatgat gtcggcattg acggagcaag cagaaaaaag cgagacacca gaaatgtttc
     4861 gcaagcagtt gaaaatggta gcagagatca tcaacgcttc ccagtctgag atgcgtgcgc
     4921 tgcttcttca tctacgtcca gtcaatttag aagagaaaag tttaaagcaa ggtatcgagc
     4981 agttattgaa ggaattgcaa aataaaatcc agatttcgct gaaatgggat gtagaagatg
     5041 taaaactatc tagttccatt gaggatcact tgttccgaat cgttcaagaa ttgttatcaa
     5101 atacattaag acatgctaaa gccaatgaat tagaggtata tttgcacaaa atagacaata
     5161 atcttttgtt acgtattatt gacgacggaa cagggtttaa tatgaatgaa acgaaaacgg
     5221 gaagttatgg attgaacaat atcaaagaac gagtagctgg tattggtgga acagtaaaaa
     5281 tcattagttt taaaggtcaa ggtacaagtg tagaaatcaa ggtccctttg atgaaggagg
     5341 catagataag gatgataaaa gttttattag tagatgacca tgaaatggtg cgcttaggcg
     5401 tctcttttta tttatctatt caagaagaca ttgaagtaat tggagaagct gaaaatggac
     5461 gacaaggcta tgagaaggcg atgacacttc gaccggacgt cattttaatg gacttagtca
     5521 tggaagaaat ggatggtatc gaatcaacaa aagcaatctt aaaagattgg ccagaagcta
     5581 aaatcattat cgtaaccagt tttattgatg acgaaaaagt ttatcctgcc attgaagcag
     5641 gtgcagcagg gtatttgttg aagacctcta ccgcacatga aattgctgat gctataaggg
     5701 caacgcaacg tggcgagcga gtattagaac cagaagtgac aacaaaaatg atggaaaaaa
     5761 tgagtcggcg aaatgagcct gtattacatg aagaactaac gaatcgggaa aatgaaattt
     5821 taatgctgat ttctgaagga aaaagtaatc aggaaatagc ggacgaattg tttattacac
     5881 tgaaaacagt aaaaacacat gtttccaata tattggctaa gctagaagtg gaagaccgta
     5941 cacaagccgc tatctatgca ttcaaacatg gtttggtgaa ataaaaacga tgataaagag
     6001 gataatcaaa agctgagaaa ataactatta tttttcttgg cttttttaca ataatgatgc
     6061 ttgcttttgt ttttgtaaag gctttgttat actgagaaaa gaagatacta aaggaggaaa
     6121 acggtgaaac aaaattttgc aattgtaggt cttggacgat ttggcggaag tatctgccga
     6181 acactgatcg aagctggtca agaagtactg gctattgata gtaatgaaga tcgggtcaat
     6241 gaatatatga atatagccac tcatgctgtt gtggcaaatg cacaggatga aatgacactt
     6301 cgatcattag gcataagaaa tttcgatcat gtaattgtcg ctattggaga agatattcaa
     6361 gccagtatct tagttactct gatggtaaag gaaatgggcg taccaaatat tttagcaaaa
     6421 gcccaaaatg agtatcatgc cagagtactt gaaaagatcg gagctgatcg cgtcgtccat
     6481 cccgagcgag acatgggaat aaggattgcg cataatttag tgtcaaagaa tattttagat
     6541 tacctagagt tgtcagataa atattcttta gcagaaatcc gagtctccaa tcctaaattt
     6601 ttcaacaaaa cattagctga attaaatttc aggcaacgtt ttgacttaac agtagtcgcc
     6661 atacgaagag tagatcaaac ggttatcgct tctcctgcag cagatgaata tgttcgtgaa
     6721 aatgataatc tactggttat tggagagaca ggcgatgtgg atattctaga cgataagatg
     6781 aatcaataga aagaaaagag aaagtaggtt gttgggatga aattaacggt aacgccaaaa
     6841 gcacaaaagt ggtttgactc agaaattgat ctgccagaaa cttacggaat acgttttttt
     6901 ggaaaagtct acggcaaaac agaggttcat gatggttttt cgattggaat gtcagttgaa
     6961 cagccagaac atcctgtaaa gaaagtggaa attgatggga cacttttttt tatcgaggaa
     7021 gcagatgaat ggttctttaa aggatatgat ttacttgttg attataatcc caacctggag
     7081 gagccaacct atcactttaa aaaacaataa aaaaagagag ggtgacacaa tttttgtcac
     7141 cttctctttt ttcttttgaa aatattgttt tcagctatta atatcctgca gacaatttta
     7201 cttgattggt ttgaagctca tgggtggcaa agtagctttt cagattggtt aaaaaaatag
     7261 ccattaattt cttttgaaaa tcaggtgtca aacctgcaat gtgggaagta atcagtacat
     7321 tgtccatttt ccaaagagga tgatcttgtg gaagaggctc ttcctcaaaa acgtctaatg
     7381 ctgcaaagga aagttgctgg ttatttaaag cctgcaccag atcttgtgtc ttaacagaag
     7441 caccacggcc aacattgatg aatacagatt ttggatcaaa gtgttcaaac atatgctgat
     7501 caaagagccc agttgtttcc tccgttagag gtaagatatt gactacgaaa tctacttcag
     7561 gaagaacttc ctttagttga tttaaagaaa aagtcttttc cattccttct gctggatgac
     7621 cggatgtatt gattccatag cagttcatgt ctaatgcttt gcattttttt gcaatcattt
     7681 ttccaatatg gccagttcca atgattagta tttttttccc ttggatagat tggtaatgga
     7741 ccgatgaacc taaccatttc atgtcactct gtgccttttg tgcttgaaaa agaccacgag
     7801 aatacccaag gatgattcct aagacatgct ccgtaatcga ttcactgtgg atgccactac
     7861 catttgaaag taaaatatgt ttctttgaaa aagtctcaag gggtagctga tcaacaccag
     7921 ctgagataga ttggacccat ttcaaggtag aagaggctaa tagtttttct tggtagtctg
     7981 agttccagcc caaactgatc tctacatttt gtagatcctc tggttgtaat tctgttttaa
     8041 agatataacc aggcgctata gtatggattt cttcgataaa ttcatcacga aatggacgat
     8101 ctgcataaat gattctttct gacatagtta atttcccgcc tttctttact ctaagtgtag
     8161 cgcatacaag aagaactttg aagcgccttg cttgtttttt gagaaagaaa tatttttcaa
     8221 ataaaaaagg ggctgtgaca aaaaaatgtc acaacctcta aaagggtcta ttgaagtaga
     8281 aataacgagt tgtctttatt tcttaattga tcgatatttt tgcccatcct ttgacagctt
     8341 gagcaaaaat aaagattcct gcaaaagcaa tgaaggcgct agtgtcaaaa gaaggaagaa
     8401 agaccatcaa gattgttgca attatagtag gcaatgaaat tgaatagatc aatgttttta
     8461 aacaatcaag aaaagtagct tttgttagtc gaaagcgtgc atagagaaaa gcagcaatgt
     8521 tggtaaatag caaagtgatc agcagattca gaaaactagg ataaatagaa aatataaatg
     8581 tcaggacttt tatccaaagt ggtatagatg cttcacttaa aaaagaacgg agctgcttac
     8641 ctgttaagtt ttttaaagat tcattggtgt agggaagttc taattggtta ttgttcaaca
     8701 aagcagaagt cgtgcctgta ttaggaagag ctatgataag tttgtttttt aacatcccga
     8761 cactaacaaa attccccatc aaatcagtag agatgtcctg ttctgttcgt tttccctctg
     8821 gatcaaatgt gaagataatg gagtttgttt ggtaaatgaa tccttctttt tcttcggttt
     8881 gaagctgccc gttttggatt gtaaaatcag gaatgcgagt agcaatcttt tgcccatcca
     8941 attgaatgtt gtttaagact tgaaaaactt gaaaagagat tggcaaagcc ataattatac
     9001 tcaaaactaa aagatagaca atggctttcc aaaatgggac atttctcgcc tcctttaggt
     9061 ctttgaatcg tataaaggaa ctgataatta attgttttgt tgtcataagt actcctcatc
     9121 tagtagtgtc atcacttgca attctagcga agtatcaagt gaaatacaag tgctttaagg
     9181 aaacagctga atgattggtg aaatatcaaa aaaatgttat tcttgtctaa agactatcca
     9241 atataaataa aatggaggaa ggaatagtgc ctacacagcc acagagatca tcaaaaaaac
     9301 tatttggagt agtggttttg aatgcggtga tcactttatc agaatttatt ggagggatta
     9361 tgtcagggag tttgtctctt gtttcagatg ctttccataa ccttagtgat acattggcaa
     9421 ttattttttc ttacggggca caaaaaatgg cccgaaaaga agcaaataaa aaacgtacgt
     9481 atggctacca gcgtttagaa atattgagtg ctttcatcaa ctcattcata ttgatcatat
     9541 tatccttgtt tttaaccgtt gaagcattca agcggttcac ttcaccagaa aagataaata
     9601 gtcatttgat gctaacagta gcagttattg gattgctcgc gaatttgttc tcagtattat
     9661 tattacgaca agaagcagat gaaagtttga atataaaatc tagttatttg catttgttaa
     9721 gtgatacact gtcttcggta agtgtgatta tcggagcaat tttgatccgt tttttcgata
     9781 tctactggat cgatccagtt attacactga tcatttcgat atatatattg atagaagcga
     9841 taatggtgat caaaaaagct gccgctattt tgatacaaag tgcacctgct atcgactatg
     9901 aaaaaatgga acaagaaatt gaagcaatcg atggggtaaa ggatgttcat catgtccata
     9961 tttggcagta tagcgaaaag ataattattt tcgacggaca tattgatttt gaagatcagc
    10021 tactaagcga gattgaaaag atatatcctc gtataatttc tttattaaaa ttaaaatatg
    10081 gtattacaca tgtgacgatc caagcagaga caaaggcaaa ggatcaaaaa aaattaattt
    10141 ttttaaataa agatgaaagt tattgaaata tagagtatga taaataagaa tttttaataa
    10201 ggtaggttgg ctcatgaagc agtatcgaca gttaaaagga tatttagaag caaaaggata
    10261 ttgggagata ctagtctatc tcttttttgg tggtttggct acgattgtaa attttgttag
    10321 ttttgctctc gcacgccaat attttgattt aagtatggcc ttatccaatt ctatttcgtg
    10381 gttttgttct gtattgtttg cttttgtaac gaacaaactt tgggtattcc attcaaaatc
    10441 accgaatttt acacatgcac ttattgaatg cggtaagttc ttcttctacc gtatcctttc
    10501 ttacggattg gacatggggg caatggtatt gttgatcaat gttatgaaca gcaatgagta
    10561 tgtggcgaaa atcatcactc aaattattgt tatccttgcg aactatattt tcagcaagtt
    10621 gtttattttt aaagaaactg aggtttttga agaagaagta ggtcaaagtg aaaaagacga
    10681 ctaggcataa caattgaaga atttccttct ctttgttact ctttgtaatg tagacattat
    10741 taagaaaaca ttgaggagga atttttttat gacttacaca ttaccagatc taccttatgc
    10801 atatgacgca ttggaacctt atattgacga agaaacgatg catctgcatc atgataagca
    10861 tcacaatact tatgtgacga atttaaattc agcaattgag aaatacccag aattaggcga
    10921 aaaaacaata gaagaattat tatctgatat ggacgctatt ccaacagata tcaagacagc
    10981 tgtacgtaac aatggtggcg gacatgctaa ccattcattt ttctgggaaa tcatggcacc
    11041 aaatgctggt ggcgaaccta caggagaaat aaaagaagcg attaatgaag cttttggtga
    11101 tttttcttct tttaaagaag aattcaaaaa agcagccgct ggacgatttg gttctggatg
    11161 ggcttggctt gtaatggaaa atggaaaatt agctattacc tctactgcaa atcaagattc
    11221 tccattgatg gaaggaaaga caccaattct aggtttggac gtttgggaac atgcttatta
    11281 tttgaaatat aaaaatgtcc gaccagatta tattgcagca ttctggaatg ttattaattg
    11341 ggatgaagta aacaaacgtt tcgaagcagc aaaataacca tttcctccgt catatcagtc
    11401 ttaaaaataa tttggaaaat aaaagagcga aagcttgcta gcgttttcta aattgggtat
    11461 aatgttcttg caaggttact tgcaagaact tttttggagg atatacattt atggaacacg
    11521 gaacggttaa atggttcaac aatgaaaaag gatttgggtt tattacagta gacggcggag
    11581 atgacgtatt tgttcatttc tcagcaattc aaggagacgg cttcaagagc ctagaagaag
    11641 gtcaagaagt agaattcacg attgttgaag gtgcacgcgg acctcaagct gctgaagtga
    11701 ctaaattata ataatttgtt gaataaagac acgacttctt agagagtcgt gtctttttgt
    11761 ctatttatga agtttttaga ttttttggct aagtaaatgc atttgttcac ttgacatgtc
    11821 ctaactaatt atcaagcaag gaacattttc ttcgttagta atggatttta aagaaatttc
    11881 agtttttgga tgttgaataa aagaccattt gtgaaaccac tagtatacat actcattacc
    11941 atgtcaattc tattacttta aaagaaaatc gttttacttt gaagaggagc atatctatcc
    12001 attaaaagat gatggtaatg caatcactag ttcgagtcat tttaagagcc atgcagtgag
    12061 ctggaaataa aacgaataga agatgggttt aacaaaagga ccagtcatag gaagaaattg
    12121 tcgtgaaatc gaatgataat ctgaacggta ggcaaagact gttaaaaaga tgggaaaggt
    12181 atgtgcgatc attttactgc acgattattt ggaaaatacc ttgttgttcg ctttttctgg
    12241 aaaaaataca taaaaaaaga taaaacgatt gcgaactgcg tgtattcggt ctttcgatct
    12301 ttttcacggt atggtatgat agttaagaat tttgagaaat tttaaaaaat aataatcgtt
    12361 acacaaaaat gttttttaaa gttttcttat gattaaaaag tgaatttgct gattgaacag
    12421 ttatatgaga caatatttta gaaaatgtga aacagaggtg tattatgaag cgatggcaaa
    12481 aagttctatt aaccttgtta ggggtattcg ttttgctgat tgccggatta tcagcatacg
    12541 gaatcagatt tatgggagaa gcgagccaaa cagtaaacaa aatttctaaa aactctaatc
    12601 gaatatcctc caaacgttct gagagagtaa gcattgatga caaagaacct ttttcggttt
    12661 tgctattagg tttagatacc ggtggattag gaagaacgga acaaggacgt tctgatacga
    12721 tgatggtagt aacagttaat ccgaaacaaa aaaaatcaac gatcatcagt ttggaccgag
    12781 atatttatac gaatatcgta ggttatggaa cggtagataa attaaatcat gcctacgcat
    12841 ttggtggtgt cgaaatggcc atggattcaa ttgagcaact gctcgatatt cctattgatc
    12901 attatgtaac gatcaatttg gatggcatgg aagatttgat caatgcggtc ggtggcatca
    12961 aagtgaataa taaaattgac tttactttgg atggtgtcca tgtaccagta ggtgaacaaa
    13021 cattgaatgg agaaaaaggt ttagcctatt ctcgaatgcg acacgaagat ccagaaggcg
    13081 atatcggtcg acaacgtcgt cagcgggaag tagtgacgaa aatcgtcaat aaagttttaa
    13141 gtttagatgg cgtaagcaat tatagaaaaa ttttaaaagc agttgagaac aatgtgacga
    13201 cggacttaga ttgggatgac atgcttgatg tagcgacgaa ctacaccccg gcgtttgaaa
    13261 caatcaaaca agaccagttg caaggcgaag gacaaatgat caatgatatc tactatcaaa
    13321 ttttaggaaa aaatgatctt ctttcgatcc aaaatgaact gaagaaacaa ttaaatataa
    13381 aaacaagcga tacattacct aatttgaaaa atgagaatgc ttcaatgatg ttctttgatg
    13441 attcggaaga tggcgattcg acaaacggtg atggatcgaa tagtgaaggg tacaccgatt
    13501 attcttccta ttcagaaaac acatatgatc agtaccaaga tccgaatacc tattatcagc
    13561 aaacagaaga tacaagtcag tactatacac caagtcaaga tactcaaagt tatgatcctt
    13621 acgctggaca ggaacaaaat ggttatggtt atgaccagaa taatgagtat ggaacaacaa
    13681 gtgggtacta gaaaaaaata gtttggttaa taaagagtgc cacctaaatt agacaaaaaa
    13741 tctaacttag gtggcacttt ttctatgtgt taaattacag gacgtctctt tttagcgctt
    13801 agtttaaaaa ataaattgaa taattcaata gagttgcgaa tagtacccat aaataataag
    13861 gaatcatgag ccacattgcc aggcgatgat gcagccacaa ctgatacata agcactgaca
    13921 agaaaataac taaaagtgta atatcgattg ctgcaatgaa aatatttctt aaattaaaaa
    13981 atagagcaga ccatataaaa ttaaaaatga actgaatcac aaaaagaccg acaagattgt
    14041 ttcgctgttt tttgttgttg tgattaagta acaaataaag agaaataccc atcaatatat
    14101 acagtaaggt ccacatcggc ccaaatatcc agctaggtgg tgcaaatggt ggaagttgca
    14161 aagaatagta atattttccg ggatttccgc taagcaaccc tgataaaaag cctaatatga
    14221 caattcctat aacacaaacc caaaaacgaa tatcttttat catct
//
LOCUS       DADVXO010000075        14233 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.077, whole genome shotgun sequence.
ACCESSION   DADVXO010000075 DADVXO010000000
VERSION     DADVXO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.077"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(51..1598)
                     /locus_tag="KB950_001862"
     CDS             complement(51..1598)
                     /locus_tag="KB950_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBB6858080.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="KB950_001863"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="KB950_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBB6858081.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="KB950_001864"
     CDS             complement(2043..2237)
                     /locus_tag="KB950_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858082.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2463..3665)
                     /locus_tag="KB950_001865"
     CDS             complement(2463..3665)
                     /locus_tag="KB950_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6858083.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(3668..3862)
                     /locus_tag="KB950_001866"
     CDS             complement(3668..3862)
                     /locus_tag="KB950_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6858084.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(3909..4757)
                     /locus_tag="KB950_001867"
     CDS             complement(3909..4757)
                     /locus_tag="KB950_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HBB6858085.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(4962..5312)
                     /locus_tag="KB950_001868"
     CDS             complement(4962..5312)
                     /locus_tag="KB950_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858086.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            5907..6353
                     /locus_tag="KB950_001869"
     CDS             5907..6353
                     /locus_tag="KB950_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBB6858087.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            6977..7969
                     /locus_tag="KB950_001870"
     CDS             6977..7969
                     /locus_tag="KB950_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858088.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            7985..8869
                     /locus_tag="KB950_001871"
     CDS             7985..8869
                     /locus_tag="KB950_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858089.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            8918..10525
                     /locus_tag="KB950_001872"
     CDS             8918..10525
                     /locus_tag="KB950_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6858090.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            10526..12010
                     /locus_tag="KB950_001873"
     CDS             10526..12010
                     /locus_tag="KB950_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBB6858091.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            12187..13179
                     /locus_tag="KB950_001874"
     CDS             12187..13179
                     /locus_tag="KB950_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6858092.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            13231..14100
                     /locus_tag="KB950_001875"
     CDS             13231..14100
                     /locus_tag="KB950_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6858093.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         4348 a         2427 c         2882 g         4576 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttgaaaaacg ttgatttatc
     2341 ggcattcccc atccatatta tatttacgtc ttattcccac tcaatatgag tagcctcgta
     2401 aagaagctat tatatcagtg ctttttcagc ctattcctca ttttggtagg gaaatggtag
     2461 ggttagattt ttactgcttg atttccatta aagtttgcaa ctttagattt tgttgcacag
     2521 gtaaatttaa tgtttcctgt catttgttct gcaattcctt catcattacg gctccataga
     2581 tgagcgtagt gctttaaagt gatttcaggg ctagaatggc ctaaccgttg cgacacgacc
     2641 agaatgtctg cgttaaactc attgattagg tagctaacat gagaatgacg taatcctttt
     2701 ccttgtatta ggggtacctt agctagtttg gcataacgct gaataattcg ttgaacagtt
     2761 gaacgataga gtgggaggtc agtgtagcta agaataaatt gttctacacc atgatttttt
     2821 tgaataactt tccacttttt tagcacttca attgtatcat cgtccagaga aatgatccgc
     2881 aagccgcttt cagtttttgt gtaaggtttc cgaataaaat ctttttgatt tgtcatttcc
     2941 aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca cgtcattcca attaagtgct
     3001 aatccttcgc ttacacgaac accagtcatg taatatagcc agatcattaa aaaacacata
     3061 tgttcgtaga aatcatcttt ataaattttt gccaaaactt cttcaaattc ttcttttgtc
     3121 cagtaaggaa cgattgattt tccttttgga attgcttttg ttcttttgga aatattctca
     3181 ttcagaaatt gcagggtgac cgcatagtct aatgatttac gaaacatacc gtacataagt
     3241 gaactgtatg cttgtgagta tccgctatcg ttcaataacc aaattcgata attttcacaa
     3301 tctcgcacat tgatttcacg aagcttctta tcaccgaaaa attcagtaat ctgaatcaat
     3361 ccacactcac gagaactcca agtacttttt tgtacgctag ctttgtagtg tggaacgtaa
     3421 gactgctcca taaattcttt gtaagttaaa tcataattcg caaatccatt tacaaccatg
     3481 tattcatttt ttatacgcat gacttctttg tgtgcttcac gagctgatga gaatcgtttt
     3541 cctagagaat cttttgttcc tttcttctgg atcctctttc cagtcacttg atctgttcct
     3601 aactcaacta gataagaaaa gcgaccattt ttatcttgat acacacaagg gtattttgtt
     3661 ttcatagtta ttcctcctgt gaaagaggaa cacctataat ttcttcaatt acttgtcggg
     3721 gtactacccc aactctttta ttattataat atggatatcc ctgatttacc attcttgttt
     3781 ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa tcctagagcc tctaaatctt
     3841 ttcgacaaat tgtatttttc attgtataaa ctcactttca aagaaaacac tatcattggg
     3901 taatagtgtt atttctttat ttttgtataa tgaacggtac tttcttgaaa atccatcact
     3961 tgttcaggac ttcccaactc atttaaagct aaagcattgt aaaaaaatgg acgtctaact
     4021 atatattcgc ttgtatatag tatatcttga ttcgtggaat catagggttt ctttaccatc
     4081 tttttggtga cagttggtag tttgagattc cgggacttga aaaaagcttt tttcttatgc
     4141 tctttcaatt ccagctcttt atcgaaatat ttgctgatat agcgcccgcg attttcgata
     4201 ctgtctacat cgatcttgtt gatcttaacg aaaccctgac cccaaatcgt agttaaccgg
     4261 ttaaatgaga taaaggggaa atcaaacaag ataatatggt agtggattgc accgcgttgc
     4321 tgcttttccc acgtggcgag atactttaaa tttgcttttt tagttttata gaggtcataa
     4381 ttcagtcgtt tgataaattt tttaaattca ttatttgcat actcaatgtc ttgaatgttt
     4441 tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa aattcgtgtc tacaaggcgg
     4501 gcaatctcaa atcgcatact ttgataatgt ttctgtttgc gttttaaact gtcatattgt
     4561 ccttgagcgg ataattcatc atagtttttt cttttttcaa gagtaatatc attttcataa
     4621 atccaagcta atttttgttt tcgttccaga tttacttcgt tttcgatcgt tttacgtttg
     4681 ggagtaatga ttgcttcata atcataaatt tcaatatacg tgggagtttc aatgattttc
     4741 tgattataag cttgcaaaaa tcatcactcc ttgttgcgta ttcgattgtc aaagaaccag
     4801 tcattttctc ctgatgttga tctataaatc aagttaaggg ggaaggcctg cgtagcagcc
     4861 cttctagcct atggggtgtt gggcggtctt aacggacaga aacggctctg tgggcgctgc
     4921 cccgccgttt cgtcctaacc acccaacact ccatagtata gttagttttg tgtagtggta
     4981 ccttgttttc tgggaatcaa agttgccgta tcagcggttg ccgataaccc ataactgaca
     5041 aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat tggtaaatgt aattatggct
     5101 aaaccttgtt gggtaagttc ttccggcgtg atcacaggat gttcagattt gactttgaca
     5161 ctgatttttt cgtagcgaag ttttggtagg atacattcgt agctccaacc tagaatttcg
     5221 ccctcctttt gccagcgagt gacattgacg acttggtatt ctttttcgag tgtttctttt
     5281 ggtaaactga gatcggtaat ttttattggc atcacaattc tcctttttta tacgaaagtt
     5341 tatacgattt gattaaaaaa tatagatggc catctacgac ttgtattata tacgagaact
     5401 tatacgtagt caagagattt tctaaaaaaa tatatacgat tttttatacg agtgttataa
     5461 tgaaaaaaat gataaggagg aatttgaata tgtttggttt agtacaacct aataaagtga
     5521 aggtgggcca acgaatcaaa gaaataaaag acggaatgtc tctctctttt acagaattag
     5581 ggaatcgatt aggtttaaaa aagccgacga tcagttccta cgttcaaggc tatgcgttgg
     5641 ctcctaagag tgtgatcaac caattatcta gtatcagcgg aaaacctgtc ggctggtttt
     5701 attttgggca tgtggaagaa tatatccgag agtatttgtt tctcattggt cagaaaaaaa
     5761 tagtggacga ttatcctgaa atcattgaga aaataaagca agaattttat acgggcgagt
     5821 tcaaaaatcc tgcttgggag aatgagctag gctatccttt ggaggaattt atccaagaat
     5881 gttttgtaga atattcgagt gaaatcatga aggactatgt cgtacagatt acgaaagagg
     5941 tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa agcaaaagaa gaagctgtca
     6001 cgattctttc atctgggatc attgactata tcgagatgag tggtgagatc aagtatggag
     6061 agaaagatcg cattgcgaaa atggtgaaag aatcaataaa aacctttgat gtgaaacaag
     6121 acctgacatt taatgattcc tatttgattg gaaagttgat caatattctt ggtacagatg
     6181 aagatacgga gctgtttctt tcttctttat caatgatatt tactgataag catttttcca
     6241 ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc gatgcgacca gcgttaatca
     6301 aactctatcg ggagaaaaca ccagatgaat tttatgattg gtttgagaag taaaataaga
     6361 aaagtggctt agattacaag ttggtgaaaa aatttttttc agcataacat cagtaatcta
     6421 taaaggggtt gttgattata ctgtagaagt taagtactca agagattttt tgtacgtaaa
     6481 caaagcaaaa aagggtgcag tttcactaga aaatgtactc ttttttgtga tttttcttag
     6541 ttatagagtt tttttgaaac tttaaaaatg agaaaacata ttgattttaa agagtgtaga
     6601 catagcttct tttgctaaat agattaaact tactggtgag tggtcttgaa catactttta
     6661 aatcacattt tactgagttt tattggattt tataattcat tggaaacctc gtacttaatc
     6721 tgtttttatc tatcgactag aatctgtata tattccagct atcaatgagg ccgcagaaaa
     6781 gggtgcttaa aaataatctg ttgtttaaaa tattattatc agaatactta gatacaaaat
     6841 tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg atatggtaat ctataaacga
     6901 aaatagtggt aacgtttcca aaatgcatct tcgttaaatt tttatttttt taaacgaaaa
     6961 tggaaacgtt tctaaaatgt attgggagga gattgaattg gaaaagacat tgggaccagg
     7021 tggtgagata agggcggtac aacgggataa aaaaaatatt tcgaagtggg aaaaattaaa
     7081 gaaacagttg cggcgtgata agtggatgta cttgttgctc ttaccaggta tcatcatgac
     7141 gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt gcatttaaag actataatcc
     7201 tatgctagga attttggaaa gtccttgggt aggactagat cactttaggg aatttatcag
     7261 ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa attagtattt atggtttatt
     7321 gttaggtttt tttccaccaa ttattttagc actgagcttg aatcaaatta tcaataaaaa
     7381 aataaaacag aaaatccagt tgattctata tgcgccgaat tttatctcag tagttattat
     7441 tgtggggatg ctgtttttgt tcctatccgc aaatggacct attaatgggt tagtagaaaa
     7501 ggttacgggg caaccaatta tgttcatgtc taatccagct tatttcagaa cgatttatat
     7561 tttatcggga atttggcaag gaatcggctg gtcatccatt ctttacaccg caacgctggc
     7621 gggagtcagt accgatttat atgaggcagc aaatatcgat ggggcaaata ttttacaaaa
     7681 gatttggcat atcgatttgc ctgcattgag gcctgtgatg gtgatcagct tcatcttggc
     7741 cgccggcggt attatgaatg taggatatga aaaagcctac ttgatgcaaa cttcaatgaa
     7801 catccctgcc tcagaaatca tttccactta tgtatacaaa gtcggcttgc aatcaggtga
     7861 ttatgcctac tctgctgctg ttggtctgtt caatatgatt attaatgtcg ttttattact
     7921 gattgtcaac aatataacca agcggctaaa cgatggtcaa ggcttgtaat gaaaggagaa
     7981 aattatgaat agtgctttat caaaaatcga ccgtcaaatc ttatggttga atcgagtgat
     8041 tgtaactttt ttagtcgtca tcaccattgt tccgctgatt tatgtactgg tagcttcttt
     8101 tatggaccca gctactttga tcaataaagg tataagcttc aatccgaaag attggacaat
     8161 acagggatat caacgggtat ttgcagatga ttccatttta cgcggttttg taaattcgtt
     8221 gttttactct tttagcttca gcttattgac tgtatttatt acgatgatca ctgcgtatcc
     8281 tttgtctcga cctaacttgg cggggaaaaa gattattatg actttttttg tcattacaat
     8341 gttcgtgggt ggtggcttga tacctaccta tttattggta aagaacttgg gcatgttaga
     8401 tagtgtatgg gcgattatcg taccaggtgc tataaatgtg tggaatatta tcttagcccg
     8461 tacctatttt agtgctttgc caaaagaact ttctgaagct gctactatcg atggcgccaa
     8521 tgacttgcaa atactgttca aagtcatgct tccattagcg aaaccaatta tgttcgtatt
     8581 gtttttatat gcctttgttg ggcaatggaa ctcatacttt gatgccatga tttacatcaa
     8641 agatcccgac ttacagccgt tgcaactcgt attaagaaat attttgatcc aaaaccaacc
     8701 ttcacaagac atggtgggag ccaatactgc catggcagaa atgaaacaaa ttgctgagtt
     8761 gatcaaatac gcaacgatcg tcatttctag tctgccatta atcataatgt atcctttctt
     8821 ccaaaaatat tttgataaag gtgttttagt tggttctgtt aaagggtaag tattgttcac
     8881 tatttgtatg aataaaaatt ataaaggaga ttttaagatg aaagcaaaat ggatgctgcc
     8941 aatcgttttt acttccgtac taattttggg ggcttgtgga ggctcaaaag aaaagagtaa
     9001 atcaagccct gattatgaac tagaaaatgt ctcttttcca ttgaaagaaa aagtatcttt
     9061 aaaaatgatg tctcaaagtg cccctttggc tcccaatgat cctaatgaca aattgatttt
     9121 ccaacgatta gaagaagaga ccaatctcca tattgattgg acaaactaca gtagtgattt
     9181 tgccgaaaaa agaaatttgg atatcgctag tggtgattta ccagatgcca tcttcaacgc
     9241 cgctgcaggt gactatgatt tattaaactg ggcggaaagt ggcgttattg taccgattga
     9301 agatttgatc gaagattata tgccaaatct gaagaaaatt tttgatgaaa accccgaata
     9361 ccgacaaatg tctactgcac ctgatggtca catttactct atgccttgga ttgaagaatt
     9421 aggcgaagga aaagagtcga ttcatacagt aaatgggatg gcttggatca acaaggagtg
     9481 gttggataat ttgggtcttg aaatgccgca aaccactgat cagttgatga ctgtgttgga
     9541 ggctttcaag acgcaagatc caaatggtaa tggtgaagca gatgaaattc cttttagctt
     9601 tattaacaat ggaggaaacg aagacttaaa atttttaatg ggggcattcg gtctaggtga
     9661 taatgatgac cacttagtcg ttgataacga tggtcaggta caatttactg ctaacaaaga
     9721 agggtataaa gaagccatca aatatttcaa tgaaatgaat aataaaggtc ttattgataa
     9781 agaggctttc gaacaagatt ggaatacgta tgttgccaaa gggaaagaac ataagtatgg
     9841 cgtgtacttt acttgggata aagcaaatat cacgggtgca gatgatagtt atgacgtctt
     9901 gccagtctta gcgggaccag atggaacgaa acatgttact cgtacgaatg gcatgggttt
     9961 ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac ttggaactta cagctaggtg
    10021 gatagataaa atgtatgaac cgttacagtc tgtccaaaat aactggggaa cctacggtga
    10081 tgaagcacag gcgaatattt ttgaattaaa ggatggtatg ttaaaacatt tgcctttaga
    10141 ggggacagca ccgggggaac taagacaaaa aaccgaagct ggaggaccat tagcagttct
    10201 tgattcttat tacggcacag tcactactat gccagatgat gcgaagtggc gtttagattt
    10261 gatgcatgac acgtatttgc catatattac agaagacaat atctatccac gtgtcttttt
    10321 agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg gatatgtggg attacatcta
    10381 tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat gaagaatggg aagaatacct
    10441 tgctgaattg gaacgtgtag gtttggaaga atggattcaa ataaaacaaa ctggctatga
    10501 taattttttg aagggagcac aataagtgtt agtagaagaa acggcgattt tggataaagc
    10561 aaatcgattt attgaaatga tgcgaggaaa agttaaccct cagtatcgac aaaactatca
    10621 tttttccgca cccatcgggt ggataaatga ccccaatggg tttatatatt acaaagggca
    10681 ttaccatctc ttctatcaat actatcctta cgatagtcga tgggggccca tgcattgggg
    10741 gcatgcgcgc agtagtgatt tggtccattg ggaagatcta ccagtagcgt tagccccaga
    10801 tcaaccttat gacagcggcg ggtgtttctc aggaagtgca attgaaagag acggaaaact
    10861 atatttattt tatacaggct gtcaagaaat tgatggtatt agccatcagc atcaatgttt
    10921 ggcggtctct gaagatggta ttcattttag caaatacagt ggtaatcctg tggtagcaga
    10981 aaaacagcta ggtgaaaatg ggctaataca agaatttcgt gatcctaaag taattgttca
    11041 caacgatggt tactatatgg tagtagctac taaatctaca cagtctactg gtagaatagt
    11101 gatgtttcaa tcgtcagatt tgtatacttg gcacttttac tctgtgctgc tagaagggac
    11161 ttcagagcaa ggaataatgt gggaatgtcc tgatttattc catttagacg gacgagatgt
    11221 cttaatcatg tctccgatac aaatcaagaa gaacggactg gaatatcata atatcagttc
    11281 taccatggct tgtattggtg aggtaaactg ggaaacaggt cagatgatag ttgataattt
    11341 ccatgaaatg gattttggaa tggattttta tgcaccacaa acaacaagag atcctaatgg
    11401 tggacgggtg atgattgctt ggatgcagat gtgggatcgt acattgccta cacatgagtt
    11461 aggccatctt tgggcaggct ctatgagttt gccccgagag ttaagagtga aaaaaaatcg
    11521 attggtacaa aaacccatct caactgtata tagtcattta gtttatcaac aaggcagtga
    11581 aaatattgca gtgactgaca catcagtcgt atttcgaaac gcgctgtctg attcttcata
    11641 tttacatcta gtcgcagatt tggagaaagc tgaagttttt acaattgaat tactgcggtc
    11701 taagacggat tgtctcaaac tagcctatga cactgctaca caggtttttg aattggatcg
    11761 tcagaaacta ggaagagagc ttacaggggc ggaagaagac cctctgaatg tacgaaaagt
    11821 tgaagtacct ttgattgata atcagctcgt tcttgagatt tttagagata cctcatcaat
    11881 tgaagtcttt attaatgggt tagaagttat gacggctact ttctatgagc tggaaaaagc
    11941 tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt caagcttttg aaacagcgag
    12001 tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt atttaatagg ttttgaagga
    12061 taatgttttt agaaaagaac agatagctag atttcccatg gatttgctat tagtattttg
    12121 aaaaaattag taatttatga caaaattcat tatactaaac ttgtgcagaa atgtgaggga
    12181 tcaactatga aagcaacaat gaaagatgtc gcaaaattag caggtgttgg agtaggaact
    12241 gtttcccggg tattgaataa cggttctgtt aaagcttcta ctagagaaaa agttgaagca
    12301 gcaatgacag agttaaacta tcaaccagat ttttatgccc gggggttaaa gatgaagcat
    12361 acgaacacgg ttgctttgat tctacccacg gtatggcatc cattttttgg cgaatttgcc
    12421 taccatgtag aaacagcatt agaaagacaa aactacaagc tttatttgtg taactccagt
    12481 ggtaatagtg aaaaagaaca agaatatatc cagatggtca gccaaaataa agtagacggc
    12541 atcattggaa ttacttattc agatatcgat gaatacattt cgagtaatct gccttttgtt
    12601 agtattgacc gttatttcac tgaagaggtg atttatgtga ccgctgacaa tgcggccggt
    12661 ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc agaatgtagc atatatcggt
    12721 gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa aatattttga aatcgagtgc
    12781 cgtcaactgg ggatttccta taaagcgtta gatatgttag aacctttaga ccatctctca
    12841 gaacgtgtaa caaatttttt gttagagaat cccgatatcg atgggatttt tacgatcaac
    12901 gatccaatgg cattagctgt cattgatatg cttgaaaaaa tagggaaaaa cgtaccagag
    12961 gatgtccaag ttatcggatt tgatggtcaa aagatgacag aaggagatcc ttatctagtc
    13021 tcaacgattg cccaacctac aaagcaaatg gcggaagtgg cagtggcaaa attaattgct
    13081 gtcattaatg gagagacagt agaaaaacgt accgtattgc cgattgaatt tggcgaaggt
    13141 gacacaacga aacctttagt gagaaacgat tttgcttagt gcaaatacgt ttcttttttt
    13201 gaaaatgagt gaatgaaagg tgtagataaa atgttatatg gtggaataga agccggtgga
    13261 acaaagtttg tctgtgggat tggtaatgaa caattacaag tgatagaacg gaaaagtttt
    13321 cctaccacaa caccagaaga aacgatggag caagtgtttg atttctttga taagtacaaa
    13381 ggaaatatcg catcaattgg tgtaggagca tttgggccta ttgatattca aatcgaatca
    13441 agaacttacg gatatatcac caacacaccg aagttagctt ggcaaaatta tcattttgta
    13501 ggagctttgg aaaacgcctt gtcggttccc atctattgga ctaccgatgt aaatgctgct
    13561 tgttatggag aatatatggc agggtatgga aaaggtaaaa atagtgttct gtattacacc
    13621 gttggtacag gtgttggtgg tggtgctgta gtggatggga aaatagtcgc aggttttagt
    13681 catcctgaaa tgggacatat gctcttacaa cgtcataatg aagatcaatt tgaaggaatc
    13741 tgtccttttc atcatgattg tttggaaggg ttagcatcag gacctgcgat tgaaaaacgc
    13801 tttggaagaa aagcccagac tatctcattt gatcatcctt attggacgat tgaggcagat
    13861 tatcttgctc agtgtgtgta taacacaacg ttactatatg caccggaagt aataatttta
    13921 ggcgggggtg taatgaaaca gccgcaacta ctcgagttga ttcgccaatc gtttaaagaa
    13981 aaaatgggtt cctacgtgga tacgcctact ctcgaagaat atctactaac tccaaggctt
    14041 gctgacaatg cgggcttgat tggctgccta gctttagcaa taaagaaact caatcattag
    14101 aatagtattt attagaacta ttcataacat accggaagtt taaaagggta ttctgaaact
    14161 taaatagtct caattcattc ttctttcttg agaatctgag tagagacttt tattctgttt
    14221 caaattaata taa
//
LOCUS       DADVXO010000076        13699 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.078, whole genome shotgun sequence.
ACCESSION   DADVXO010000076 DADVXO010000000
VERSION     DADVXO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.078"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(54..746)
                     /locus_tag="KB950_001876"
     CDS             complement(54..746)
                     /locus_tag="KB950_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6858094.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1183..1752)
                     /locus_tag="KB950_001877"
     CDS             complement(1183..1752)
                     /locus_tag="KB950_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6858095.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1826..2149)
                     /locus_tag="KB950_001878"
     CDS             complement(1826..2149)
                     /locus_tag="KB950_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858096.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2303..3115)
                     /locus_tag="KB950_001879"
     CDS             complement(2303..3115)
                     /locus_tag="KB950_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858097.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3468..4316
                     /locus_tag="KB950_001880"
     CDS             3468..4316
                     /locus_tag="KB950_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBB6858098.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4461..5402
                     /locus_tag="KB950_001881"
     CDS             4461..5402
                     /locus_tag="KB950_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBB6858099.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5392..7563
                     /locus_tag="KB950_001882"
                     /pseudo
     CDS             5392..7563
                     /locus_tag="KB950_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7675..9846)
                     /gene="copB"
                     /locus_tag="KB950_001883"
     CDS             complement(7675..9846)
                     /gene="copB"
                     /locus_tag="KB950_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBB6858100.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9866..12052)
                     /locus_tag="KB950_001884"
     CDS             complement(9866..12052)
                     /locus_tag="KB950_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBB6858101.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12052..12261)
                     /gene="copZ"
                     /locus_tag="KB950_001885"
     CDS             complement(12052..12261)
                     /gene="copZ"
                     /locus_tag="KB950_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBB6858102.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12274..12714)
                     /locus_tag="KB950_001886"
     CDS             complement(12274..12714)
                     /locus_tag="KB950_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBB6858103.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12788..13327)
                     /locus_tag="KB950_001887"
     CDS             complement(12788..13327)
                     /locus_tag="KB950_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6858104.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3864 a         2858 c         2437 g         4540 t
ORIGIN      
        1 agtccattaa atagttcaac ttttggggtt cagttcaaag agggggggat tttttatcgt
       61 tgtggaatat ttaaatacca acgcttgtca tgaaaatctt gtgctccacc cttagtattt
      121 ccttctggat cgtttgtcgc acgcatcatg acgtatactt tcttattagg gaaatcacgc
      181 atattgaaag atacatgata gccaacattt cctaaagtat tataagcttg atttacgtct
      241 ggtctataaa tcccatcagc tcttactcta gctagttctt ttccagtatt gtagtccata
      301 atgaaaatat actcgtattt ataattagca atgtgccatc cagctacatg caagtttgcg
      361 ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg ttccatctgt cagcgtagaa
      421 tttgcagcac ctgctcgagt tggatcaatt actggtttat cctccgaagt agttggatta
      481 tcatccgtaa aaccatgagc caaatcatag gctagttttt ctttacttac acccatttca
      541 gaaagataac cgtaaggatc tgtatgatcg ccccaaatat tttgtgttac ccataaatgc
      601 gatttgattc ctggttggtt ataaggagtg tctaatgtta atggaatacc atatttcatt
      661 gctgaatctc ttgccaattc aacgtatact ttatagttct tttcaaaagt ttctttatca
      721 tgtgtatgtt gtaactcgat ttgcacagga cttttggctc acgaggttgt cctactctac
      781 atcttgctga tagatatgct gaaaggaaaa caccaacatt atgttttgct ttttcagcta
      841 gaacgagaca agcaggaata cctttagaag atatcaaagg ttttttaggt cataaagatg
      901 tttccactac acaagtctat gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc
      961 aactagaaaa ctatatagaa gagcaaataa aaaagcactc aaattgagtg cttttttaag
     1021 ctcactatca ctaaaactat cactaatttt atttataagc gaacatgttt ctatcactga
     1081 ttttttaaat ctagcgaaaa acccttatct taaaggcgtt cgttcaattc tttggcaagg
     1141 tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt tcttatagct catgtagaaa
     1201 aattttcttg atgatgattt tatccatcaa ttgagattga ctgttttgct ctcctatgat
     1261 ttcagctatg attgtccgat tttgcggttt tcttcttaac tggtagttga ttttcccagt
     1321 ttccatcaac tcgctaagca tatccacgac tccgccaaat ccggtctttt tcgtaataaa
     1381 acctagtgct accattcgtt catctagatt ttttgctgta atcgtcacac cagtaatgag
     1441 cgagtgctgt aatgtttggg ccagctcttc catcccttca gcattggata cgacggaagc
     1501 tgttttgtta ataggttctt cgtttatttc ttttggttct ttcctctcca ctttcactgt
     1561 ttgacgaagg acatctagtt cttgcaagag cgtttcgtat tgttcttggg tcagcatcac
     1621 gccagctact ttttcacgat taaatatata tacgccagtt cccgcgtctc gcgcttgagc
     1681 aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa gatgtgggaa cttctaattt
     1741 ctttaattcc aatggatttc ctcctttact aatcgctttt tattatagca cagactgact
     1801 tgttttgatc ttttttccaa aggttttatc ggtttttgcc taaccagtaa ttgatatcgg
     1861 taagaagtct ttcgatacca aatgaaggat ggtacagata cgttttttga tcttcgttat
     1921 ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc ataccgaggc ggcttatttc
     1981 gagtacatac atgtatagag atccgttttc gatgtcttag cgttttgttc agtaccccag
     2041 caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa gacgattttc acttcaggag
     2101 ggtggacatc taaataccct cgttcctgga gtgttttcag taaaagcatc ccccactcta
     2161 ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa ttgatatagt ttatctttgg
     2221 aattgggaaa tttttctaat gtttcctgat aataacgaac ttcttctatc agccggtcat
     2281 ggataccaat tacgtaacga agtcattctt ggtaaagtac ttttgtcagc tttgatttgc
     2341 tttcgctctc aaattcgcta ttcagccaaa acagcgaatc aagcttttca gtcacaatcg
     2401 tcaatttccg atagtcttgt gtctcttcta tccgtttcca ccgcttttca gtaatctggt
     2461 tacagctcaa gcgcaaaatt gctagataat ttttttcttt tattggcaaa atctgctcct
     2521 ctctaaatag caattcagga aacaattcaa agaatagtgt tcgactgtct cctaaaatct
     2581 cgatttctgc cggatccttt ctttgcttca ataattggat gaacatctca gaaaaggact
     2641 gctctccatc atctgcaaaa aaatgcggaa aatctttcaa aaagcaaaac agcagatatc
     2701 ttacttttct ttctgaacca aagatctgtg cttttttata agaaaaagaa aggtggtact
     2761 cgccaaacca ctttctcaat cgctcttgcg tagaacgatc gtacttctgc tcgtaaggtt
     2821 cattcaatag cagatttttc agtaaacaaa acgggggtga ttggatcaga aaaacaaaca
     2881 agagataagg ttcgctatat ccttgatcat aaactaactg aacggttttc ttatgaagaa
     2941 tcagctgaat gggacagtcg ctttccgcca aacgtttcag atgccgatcc actgttttaa
     3001 catcgatttt cagttcttca gctaaatcag cttttcgcca gatcaaatct ttcattagat
     3061 tgattagtga aaataaattc tgtgcatttt ttgataatag tttttgccat ctcatcgatc
     3121 atttctcacc tgatttctaa acatattgtt tgatcaaaat gggttcactc ctaaaaagat
     3181 aaaaaactcc ctttctattt atttaaatac gcaaatcttc ctcattattt tttattttac
     3241 tcaatttaat cgtattctca tttcaaaacg aatgtacttt aaaaaacata ataagatcac
     3301 tttgcttgta ggatgtattc ccttccatta caatgaaata aatagcgatt ttatgagaag
     3361 gtgatgtgaa aatggaagaa aaaatagaag aatccaaaca aaaaatcgat cagttgatat
     3421 aagaattaga aaaagcggtc atcggcaaac gttcaactat ccaattgatg atcgtggccc
     3481 tattagcagg tggtcatgtc ttattcgaag atattcccgg tgtaggtaaa acgttactgg
     3541 tcaaagcact agcaaaagct gtacagggaa ccttcagcag agtccaatgt actcctgatc
     3601 tgctgcctag tgatattctt ggtttttccg tatacaattc acaatcaaaa gagtttgaat
     3661 ttcgtccggg accgatcttt accacgatct tgttagcaga tgagatcaat cgtacgactc
     3721 ctcgtactca gtcagcactt ttagaagcaa tggccgagaa tcatgcaacg atcgacaaca
     3781 atacataccc tctagacaat catttttttg tcttagctac gcaaaatcca atcgagtacg
     3841 aaggaacata tcctttgccg gaagcacagc tcgatcgttt tttattccga ctacaaatcg
     3901 gttatccttc ttttgatgac gaacttttgc tacttttgga taaattcgaa aaagtgttgg
     3961 ataacttaaa tgaagtattg aacagttatg aaatagaaga aataaaaaca aatgtggcga
     4021 atgtctttgt cagtcccgaa gtggcatctt atgccttgca gttagtgtca gcaacaagaa
     4081 gtcatgctgc catccagctt ggcatcagtc cacgaggaag cctttccttt attcaggcag
     4141 caaaagctta tgctttaatc catgggagaa attacgtcac gcccaaagat ttgcaggatt
     4201 tagttcctta cgtgttttcc catcgcctga ttttttacga tcgttcattg aaggaagacg
     4261 aaaagaaaca aatcattcat actcttgttg cacaaattcc tattcctgtg aggtagattg
     4321 aggtagacaa tggaaaaaat caaagattat ttcattaatt ttgtgttatt tctggtatat
     4381 ttcatcattg tgctttttgc tcttgtattc aacaattctt taggctggtt cttgttggcc
     4441 ttttttactc tttatgtatc atggagcttg atttcattaa gcagctttct aaagaaactt
     4501 tcggttactt gtacattgca gccactcgtt tatcgcaaaa aaagtatgca ggttattttt
     4561 tccatagcaa aaaagagaac tttttcttta cctctccctc gtttacaaat cggcttccca
     4621 gcaatctttg cagaaaaaaa caagagattt tgattcttac aaataaaccg caagaagtcc
     4681 aattgctttg gcagccaaaa gaaagaggat gccatgaaac attaactgtc atttttacag
     4741 cctatgatag tctaggactt ttcagaaaaa gtgcaaaaag agagatctct tttcctgtga
     4801 ctgttctgcc tgctttttgt aagattcaag cagaaaaatt acagagagtt ctggaaaaaa
     4861 aacagcagct gaacgcgtac caaagaggtc ttgattttcg ggaacatcgc gcttatcgtc
     4921 caggtgattc tttcaaccga atcgattgga aattgtctgc tcatactcaa gagtggttat
     4981 accgtgaata cgattatcaa gaagaagaat gttctcctct tttttgcttt tggggttcgc
     5041 ccagtccaaa atttgaacct ctgctagact tgtatttttc tttatggcat ttgagaaaag
     5101 aaaagcagcc ggaacttctg atattaggca accgcgcctt tcatggcaaa gaccccggtc
     5161 atacctattt tgcttctttg gaagctggcg atgagaaagc ttttttcgca cacttgaaaa
     5221 aatatgcaaa aaaacaaatc gtcctctttg ttcctgagaa ttctcctgaa gtctcagaag
     5281 ctattgaaac attgtcaaaa gatcgaacgg tttatcttgt ttatttcgta gaaaaacaac
     5341 tcatggtcca agaaaaagac aagatatgtt cattgccagg aggtgaatgg attgacgatt
     5401 aaacgggaaa aatggctgcg tggcgtttac tcctttgtga tactaagtgt aggtgctccg
     5461 atatttgtag tagctaatca attgcaaaac agctaccttc ttatcctaac tattggtgtc
     5521 ctatgcctta ttgtctgctg tattcctaaa aaatgggtac gtttgctgct ttctttgcca
     5581 gtaatgatag gttgtttgtt tttatatttt ccttcgaaga cattttccat attatggatc
     5641 ttacagtttt tttcgtcagt cacagaggaa ttcttccatc tatcccaagg taaactttat
     5701 tatttgccag aaaagacagc tttttttctc ataatgttgc tgatctattt atttattaca
     5761 ttgaccgtgg attacgacta ttggtacgca ccctttcttt ctttgatggc ttacttcatg
     5821 atgcttaccg tttttcggaa aaaagattta ctgtacgaaa tgattgcggt agtaactgtt
     5881 ttgttcgtgt atctggcagc aaggcaattt ttctccatcc ggttgttgtc gggaaacact
     5941 cgattccagt cattgactac agttctgctt atttgtttct taacttttag cgctatcttg
     6001 cctttatatc ttccgaaagt acaccaatct ctcgaggaaa cgagtgaacc gatccgagaa
     6061 tatcttaatg atgaagggct ttatgacaca ctccatgagt atcaattaac cggaagtgca
     6121 agaacaggct tcagtgagaa tgatgaacag cttggtggac ctttatatga taatggcgaa
     6181 gttgttttta cagcgaacca aaaaggaaat ggctattgga agatcgaaaa taaaaatcat
     6241 tacaccggtt ctggatggat agaagatcag gacgcaccca tccaaccaat ccaagaattc
     6301 cctgttgaca tcacgagcta ccaaaccgtt atagaagaca aagaaaacat tacattgacc
     6361 ctaaaaaaac aagttttctt accttatcct tatggaaata ctaccttgac tgatttttca
     6421 ttagctagtg gacaaacgct ttactatttg cctgaaagcc agcgattcat tcctgtcccg
     6481 aatgaatcga cacgttctgc aactttacaa acagaaaggt tagtttttac gagagaagaa
     6541 ttaaaaagca gtcagttaaa agctgatgat ttagaaaatc agcagacaaa tcttcaattg
     6601 ccaaaaagtt tgcctaaacg tattgaggac ttggcggtcg aattgaccaa aaatgaagca
     6661 actttaattc gtaaagtcga agctgtctaa cagtatttaa aaacaagcgg aaatttccgc
     6721 tattccaaaa cggataccgg gcatactcca aaagaacacg attatgtgga ttatttcctc
     6781 tttgattcaa aagtcggcta ttgtgacaac ttttcaactt ctatggtcgt ccttcttcga
     6841 acacttggta tcccagctcg ctggacaaaa ggttttacaa atgggctcag aacaggcaca
     6901 gataatcaag ggaatgagga atacagcatt ctcaatagtc atgctcattc ttggccagag
     6961 gtatactttc ctggatttgg ctggcttcca tttgaaccaa ccccttcatt tgccaatcca
     7021 atgggttcac aaccagttgc tgaaacaacc gaaaccgtcc agcctgctat cgaacaacca
     7081 aatgaaagcg aaaatatggc agaaactatt tccagtgaga gatctgaggt cattccatca
     7141 gaagaaacaa cactcacacc agcttataaa atcgtcttat tcagccttgg agtgtgttta
     7201 tccgtcttgt tgttctttct attgttattc agaaaatctc tgcgctggac aagttatctt
     7261 atctatcttc atttttttgc ttctactaat acgattgcat atcaaaaact tcttaaccct
     7321 ttggaaacat tggtaccgcg caaacccgca gaatctttgc aggaatatag cctgcgaata
     7381 gaacaagaat tcaagctaga tctgcgtttc attcgattaa ctactcaata tgaagcagaa
     7441 atatatggac gacaagcaga tccggtaaaa ttcgataaaa aaacctattt agaaataacg
     7501 catataataa gaaaaatggt atacaaaaaa attctgacaa aagggagccg cttcaaaaaa
     7561 taagataaaa tttctggaga atctacttct ggaatgccga gaaccagctg attgagtatc
     7621 gattactcga aaaaaaggtt gtgacacgtt ttttgtcaca accttttttc attattatct
     7681 gattttcaat gtcaaagcat ttagagctac aactaccgta cttaacgaca tcaagacagc
     7741 accaactgct ggacttaata tgataccaat tggcgcaaga attccggctg ctagcggaat
     7801 tgcaagaata ttgtaacctg ccccccacca gaggttctgg atcattttct ttcgcgtttg
     7861 cttggctaga tcaagaaaat gcaaaatatc ttttggatcg ctgtttgtca atacgacatc
     7921 cgcagaatcg attgcgatat ctgtccctgc accgattgcc atcccgattg aagcacgggc
     7981 taaacttggc gcatcattga tcccatcacc caccatgatt acttttttcc cttggtcaag
     8041 gtatttctgg acaacagctt ctttatcatc tggtaataaa ccgccataat attcttcgat
     8101 ccctaaataa tctgctactg cttgggcagc gtttttattg tcaccagtaa gcatgacagg
     8161 agtgattttt ctttctttca atgttcggat aaattctttt gcttctgttt ttactacgtc
     8221 acctaaagca acgattgcag ctagatgatt ttctaaaatc aggaagctga tggtatttcc
     8281 ttgttcttgg taaggtttca ataatgcttg ttctacgttt aatccgagac gctcaacttc
     8341 tttttcatta actattttca cttgttgttt tttgactgtt gcctctagcc cgactcctga
     8401 aagatttttt agattttcag cttgataagg cttgatttca tgttctttca aataattcat
     8461 aatgccaaca gccaatggat gattggcatt cgcttccaag gctcctagat attgaaggat
     8521 ttcattttta ttgaacgcat catcaagtac ttcgacaccc gttacagtaa attttccttc
     8581 tgttagtgtt cctgttttgt ccagcatgat cacatctaga tcattggctt gctccagtgc
     8641 attgcggttt ttcaaaagta atccgttttt agctgctatt gatgtggaac gagcaacaac
     8701 taatgggata gctaaaccta gagcatgagg acaggcgatg ataaagactg ttaccatacg
     8761 ttctaatgca ttaggaagat ctgtcaagaa caaccaggcg atgaatgcca ggatacccac
     8821 aattaatgcg acataaaaca gccattttgc cactttgtca gataatgact ctagcttgga
     8881 tttttctcct tgtgcttttc gcaccatttc cataaccttc gctagataac cattttcacc
     8941 agttccagtt acttcgattt cgatcgttcc atccccattg atagaaccgc caatcacttt
     9001 gtcattggct tgttttttga ttcctcttga ttcgccagtc acagctgatt cgtcaacaat
     9061 tgtttcacct ttcaagatta caccatctgt tggcatttta tcaccagacc ggacgatcaa
     9121 acgatcacct tcgtttactt ctttcaatga aactgtctct tcttttccat ctttcgtcaa
     9181 gcgtttcact gattctggca gtaattctgc tagtttttgt aaagcattgc tggcattcga
     9241 cacagcattc atttcaatcc agtgacctag tagcataata acgatcaaag tagccaattc
     9301 ccagaagaaa tccatgacgt gtgtatgtgg attgagcaga tttgcgataa acgaatagac
     9361 actataaata tatgcaactg taatccccat cgcgatcagt gtcatcatag caggactttt
     9421 ttgtttcaat tccatcttag ctccacttaa aaatggctga ccgccataga taaatagtat
     9481 agttgctaag atcaatacga gccaatcaga accaggaaac gttacttgaa atggaaattc
     9541 cattcccatc attggtgaaa aaagaatgat tgggatagct aggataagcg atagccaaaa
     9601 tttttgtttg aaattcccca tgtgcatgga atgatccatg ccgccgtgtt cgtgatccat
     9661 gtgcatcgaa tggtccattt gtttgctgtt tgctatatct ccatggttat gtcccatgtg
     9721 catggaatga tccatctgac tatggtcatg tttcatctcc atctgttttt catgagattc
     9781 tgtactttct ttgtttacta cttctttttt atgctcatgt tttttctcca tatcgttccc
     9841 attcattttt actccatccc ttctcttact ttttcatgtg tcgatttaag cacagggagt
     9901 tcaacaatac actgacggaa ctaaacgcca tcgctcctcc tgcaatgatt ggattcaaaa
     9961 agcctagtgc ggcaaaagga ataccaatcg tattatagac aaaagcccag aaaagatttt
    10021 gtttgatttt tctcaatgtc aacctagaca actcgattgt ttgtgcaatg gatgccaaac
    10081 tgctattcat cagcgtcaca tcggctgttt ccatcgcaat gtccgttcca cttcccattg
    10141 ccataccgac atcagctaat gctaaggccg gtgcatcatt gattccatcg cctgccatac
    10201 cgactttttt gccatctttc tgaagttttt ctacgtaggc tgctttatcc tctggaagta
    10261 cttcagcaaa gatatgcttt ggatcaatac caacttgttt accaatcgtt tcggctgcca
    10321 gtttgttgtc tcctgtcagc ataaaaacat ctaatccttt attctggagc tgcttgattg
    10381 cttgtttgac ttctagtttg atttgatcag ccacggcaat caacccgatc acttgctttt
    10441 catcagctaa aaacatgact gtttttccct gctgttctaa ttctaacgcg tgttcttggt
    10501 attcatcaaa tgataggttc aattcaatta atcgtttacg tgtacccgca aaatagcgta
    10561 ctccgttaat cgtaccgctg attccagcac ctgggtgggc tgtaaaatca atcatatcgt
    10621 atgttgctgc ttcttgcagt tttccgtatt ccacgattgc tttacctagt ggatgttcag
    10681 aagcttgttc taaagtataa aaaatagata ggacttcttt agaaccgact agatctgtaa
    10741 ctttgggctt tccttctgta atcgttccag ttttatccaa aacaatgctg tctaagtgag
    10801 cagctgcttc taatgcttct ccgcctttaa ttaaaattcc atttcgtgca ccaacacccg
    10861 ttcctaccat aatagctgtt ggagtcgcta aacctaatgc acatggacac gcaatgacta
    10921 atacagagac actatggatc aatgcgagtt gccaatcacc agtgatcaaa cctgtagcta
    10981 tgagcgtgat aaacgcgatg actaatacga tcggaacaaa aatccctgag atccgatctg
    11041 cgattttttg gattggggct ttgctccctt gagcatcttc caccatttga atgatttgcg
    11101 ctaatactgt ctgcttgccg acttgtgtaa cttccatgtg aagtagacca ttcgtattga
    11161 ttgtcccgcc aaagagcgtg tcatccggat tcttttctac aggaagattt tcaccagtaa
    11221 gcatactttc atctatcgca ctgcttcctg aaataattcg accatctgct ggaatctgct
    11281 caccaggacg gatgactagt tgatcaccaa cgactacttc ttcaatcgct aacgtttctt
    11341 ctttcccgtt tcttatcact tgggcggttt ttgtttgaag agacatcaat tgtttgattg
    11401 cattacctgt tttcgttttt gctgtatgtt ccaaatattt gccgagcaaa atcagcgtga
    11461 tgatcatact gctgctttca aaataaagat cttgggggtg tccacggaaa aatccgttgt
    11521 aaatgctcaa tgcaaaggca gccgatgttc caattgctac caatacatcc atatttggtg
    11581 ctttcgtctt gattgcatgg tacgcacctt tataaaaacg agcaccgaca taaaattgga
    11641 caggagcaga cagcactaac tggacgattg gaaaatgaaa aaagtgaaca atcgccgcat
    11701 ggcttcctaa catcatagca atcattgaca acatcaacgg caaagtcaaa accgtactca
    11761 atataagatc gaatagcatt tttttcaaat acgcttgctt ttcttctgca attttttgtt
    11821 tgtgggcttc atcgtacaaa attgcaccat agccgatgtt ttctacgctt tggattaatt
    11881 tttcagtggt cgtcccttca tatttgacac ttgctttttc agtggccaag ttgactgtgg
    11941 cattgaccac accaggttgt ccgttcagct ctttttcgat cctagcagaa caatttgcac
    12001 aagtcatacc tgtaatgacg aacgtatcca ttttggtttc tgctgttgcc atcaaatcac
    12061 ctctgcttta taacccaatc cattgattgc ttgacaaatc ttttcggatt gaacttttgt
    12121 ttcatcaaac ttgaccgtac cgtttgcttt tttcaaattt actttcactt tttgcacgcc
    12181 gtcaagtgcg cttactgttt cttctactct tgctgcacaa tgattgcagc tcataccttc
    12241 gattgaaaat tgttgtttca ttttttcttc ctcctattga tgttgtttac attcacattg
    12301 acctgggata cagttacatt cgatcgtatc tactggttgc ttttgttcca gttgtttcaa
    12361 aatctcattg atatcttctt gagtcaaagt ggcttcttct actagatccg caatcgtctc
    12421 tccaatcctt tttgcacaga tatgggaaaa aagattttcc gtcgcactgc gaactgtttc
    12481 catctccgag acagctggat aataaatgaa tttttttcct tcttgttctg tccagatcac
    12541 ttcttttttt accaaacgac ccagcaaagt tttgatcgtt gctggtttcc aatccatcga
    12601 atcagctaag atctgcgtga tttcttgggc tgtcgtctgt ccaagtgtcc aaataacacg
    12661 catgatctcc cactctgaat cactgatctt tactggcgtt ttgacttttt ccatttattt
    12721 cacacctttc gtttacaaat gtaatcatta tcttatgaat acagattaca cttgtaaaca
    12781 aaaactgtca attatttttg ctctctattt tcaatttagt taaaaattct tgtttctctc
    12841 gtcttgtttt aaggatgatc agtttatttc ttgcattagc tacttggatc agctgctgta
    12901 ttttcggttc attgttttct tcaaaatgcc agataaaaga taaaaattct aagtattccc
    12961 tgtttagttt ctccgaaaat gattcaggca tatctggccg agattttttg tttatctttc
    13021 tttttagaga acgccaaact actcggtaga tggcgagata acgcggcact ttaagccaaa
    13081 tgatcttatc tgcttcttta atcctttcct ctaatgtaga agtataattt ccttcaacga
    13141 tccatgacgg attagatacc ataaacaatt gctgttcttg caaaaaccac ttttttgcct
    13201 gaatactgta gtctgttgta tgccataaaa ggtctagcgg cagtagcgga aagtcggtga
    13261 tttctttgat cttccttgcg agggtcgatt ttcccgaccc actttgtcca atcacgatga
    13321 tcttcattct tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc
    13381 cgcaaaaaaa caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaagg
    13441 ctctataatt tatgggactg atgaatcaga attgattctt cagccccatt tttattttag
    13501 gtattaaaaa acatatcgtt ctctagtatg attaaatcac gacaaaataa acaactgaga
    13561 ggacgatatg ctatatgaat gattctatca aaaaaatgct gcgattaata gagaaagatt
    13621 tgatgattac agaggtctct tacgagacct ttcagaagaa aaagacgttg atcgtcgatg
    13681 ctgttttctc acctgctcc
//
LOCUS       DADVXO010000077        13503 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.079, whole genome shotgun sequence.
ACCESSION   DADVXO010000077 DADVXO010000000
VERSION     DADVXO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.079"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(334..894)
                     /locus_tag="KB950_001888"
     CDS             complement(334..894)
                     /locus_tag="KB950_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6858105.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1036..1650
                     /locus_tag="KB950_001889"
     CDS             1036..1650
                     /locus_tag="KB950_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6858106.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1839..3719
                     /locus_tag="KB950_001890"
     CDS             1839..3719
                     /locus_tag="KB950_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBB6858107.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3798..4025
                     /locus_tag="KB950_001891"
     CDS             3798..4025
                     /locus_tag="KB950_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6858108.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4030..4572
                     /locus_tag="KB950_001892"
     CDS             4030..4572
                     /locus_tag="KB950_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6858109.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4799..5515
                     /locus_tag="KB950_001893"
     CDS             4799..5515
                     /locus_tag="KB950_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBB6858110.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5575..7593)
                     /locus_tag="KB950_001894"
     CDS             complement(5575..7593)
                     /locus_tag="KB950_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBB6858111.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7606..8604)
                     /locus_tag="KB950_001895"
     CDS             complement(7606..8604)
                     /locus_tag="KB950_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858112.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8802..10043
                     /gene="sstT"
                     /locus_tag="KB950_001896"
     CDS             8802..10043
                     /gene="sstT"
                     /locus_tag="KB950_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBB6858113.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10190..10825
                     /locus_tag="KB950_001897"
     CDS             10190..10825
                     /locus_tag="KB950_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBB6858114.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            11016..11756
                     /gene="truA"
                     /locus_tag="KB950_001898"
     CDS             11016..11756
                     /gene="truA"
                     /locus_tag="KB950_001898"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBB6858115.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11901..13499)
                     /locus_tag="KB950_001899"
     CDS             complement(11901..13499)
                     /locus_tag="KB950_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858116.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4525 a         2134 c         2630 g         4214 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 atatagatca tagatatcct tataatgtgt ataaaaagtc gaacgatgta catctgccgt
      781 ttctgttagt tcgcgtatag tgattgcttg aatcttttta tttactaata atgtggatag
      841 tgtttcagcc aaagcttttt ctgttttttt gacgcgtcta tctgtctttt tcatttacct
      901 atctccttat ccgacatatc aaagcaaagc tgtcgcttaa tcgacaaact tatagatgat
      961 tgattataga tagtagacac tgtgtctatt ataatgtaag tataacacaa tcattgaagt
     1021 tgaggaggcg aaatgatgaa actaactttt aaacccttag aaaagaaaga ttacaaaaaa
     1081 gctatctatt ttgctgttga aggaatgaac tttgagagat atatggaaaa tgaacttctg
     1141 ctgaatcttt acggaagata ttttttgtat atggagttga atcgagcaac tcaaattctt
     1201 gctttgtatg cagaggacga gttggcaggt gttttgctgg cggagataaa aggagaaaaa
     1261 aagaaacatc gttcttttgg acagcaaata tatgttaaga tattcgattt tttacaaaat
     1321 atatttgcta aagaaagtaa aggagcttac gagacagcaa atgaacaaat gcttgcagca
     1381 tatttaaaga agcattcgcc agatggagag atcatttttt tagctgcaaa tcctgatttg
     1441 aaaacaaaag gcatcggtac caagttgcta acagaatttg aaaaaagaga gaaaggaaaa
     1501 gaaatattcc tttttacaga tagtggctgt acgtatcaat tctatgaacg acgtggattt
     1561 gaaagactaa aggaaaaaat tagcaagata aaaatcttag ataaagaaat agatctccga
     1621 tgtatgctgt acaggaaaat cattcaatga aaatttcgat agaaacgaca atagttggca
     1681 agatcacttt tagtgagtct tcttgctaac tattttttgt agtgtattct accaaaagaa
     1741 aaccgccttt ttgactaaac ttgatagtgg tcaatttttt atgatggata agtatctata
     1801 cttagtctcg taagaatgaa aggaaaagag ggagaaagat gaaattacaa aagtggatcc
     1861 aagagcatcg aaatcatatc accgccttat cagctgtgtt gattattttt ggttttatcg
     1921 gaaaatttac tggttttgtc ttgttctata acattgctct agccatagct tctattattg
     1981 cggtcatacc tattatgatc catgcgtatc aggcagtgaa agtcaaagtt atctcaattg
     2041 atttactcgt tactattgct gtgcttggtg cctttatgat cggagaatac aaggagtcag
     2101 ctatcgttac attcttgttt ttgtttggta gctatctgga acaaaaaaca ttgaccaaaa
     2161 cgcgggagtc aatcaaggct ttgaccgaga tggccccatc tactgctagt gttgtccaag
     2221 aagatggaac gactgaagaa gtggaaatcg atgacgtgga agaaggcgat atcgtcttag
     2281 taaaaactgg tggagctatt cctgtggacg gacgagtcat tgacgggaaa gggtatgcgg
     2341 atgaagccgc agtgactggt gaatcacgac aagtcgtcaa aatcagtgga gatgaagtct
     2401 tctctggaac gattttagca gatggctacg tgacgattga agcaactaag gtcggagatg
     2461 atacgacctt tgccaaaatt atcgaattag tagaagaagc acaagatacc aaatcccatg
     2521 cggaaaaatt cattgatcga ttttctcagt attatactcc aaccgtttta cttattgcgt
     2581 tggttgttgg tctgattagt cgtgattttc gtttagcaat cactgttttg gttcttggtt
     2641 gtcctggcgc tttagtcatt ggtgcgcctg tttcgaatgt agcaggaatc ggtaatggag
     2701 cgaaacgggg catcttagtc aaaggcggtg aagcaatgga tactttttcc aaaatcgata
     2761 cttttgtctt tgataaaact ggtacattga ccaaaggaac tactgctgta tccactatca
     2821 agaactatac agaggatgaa ggggacagct tagctttagt ggctcgatta gaaacgttat
     2881 ccgatcatcc tctggggcgc gccgtgatcc attatgctca ggagaaagac attgattttc
     2941 aacaattagc agtcaaagat aatcagacaa tcaagggaca aggaatcaca gctgagattg
     3001 atggtcatac agtttgtgcg ggcaacacca aattgattgc cgctcacaaa ctttctctca
     3061 caccaaagca agtacaagat ttacatcaat tacaacaaac aggcagctcg gtcattattg
     3121 tagcaatcga caaaaaaatc actcaaatca taggtgtgtc agatgtgatt cgaccagaag
     3181 tcgctgaaca attagctaag ttgaaacaag caggagcaaa acagttagtg atgctgacag
     3241 gtgataatca gatgactgct gattatgtag ctgatatgtt aggcattgat gaagtccatg
     3301 ctgaattatt accagacgaa aaggttcaat ttgtgaaaaa ataccaagaa caaggattgc
     3361 gcgttgcttt tattggagat ggaatcaatg attctccctc tttggctgcg gctgatatcg
     3421 ggattgctat gggatcagga acggatgttg ctatcgaaac gagtgacgtt gtcttgatgc
     3481 aatctaattt tgaatcgttg gttcatgcct accgcttggc taagaaaact gttttaaata
     3541 cccgtgaaaa tgtagtgatt gccattggtg tcgtcttatt tttactcaac ggcttatttg
     3601 ccgggtttat ttacatggct agtgggatgt tcgttcacga agctagtatc ttgatcgtta
     3661 tctttaacgc gatgcgttta ttgtattttg gacacgaaag caaagaagaa aaatcgtaaa
     3721 acttgataag aatcaatttt tttatggaag ctgtaatcta gaataagact atcaagaaaa
     3781 aggaaggaag aactacgatg aaaaaaatta ttatgcaatt agatacacta gcttgtccaa
     3841 gctgtatgca aaaaatcgaa catgcggttt ctcaacagcc tggggtaagc aatacgaaag
     3901 ttttgtttaa tgcgagcaaa atcaaagcaa actttgatcc agatgaagtc caccctgaac
     3961 aattagctgc agttgtggaa aaattaggtt atgaagtcca atcagcaaaa gtaaaagagg
     4021 cgtaaatcaa tgaatatagt ggaagaaaaa tttgttcaag aaacaaaaca agcagatatc
     4081 gaccatcata aaccaactgc tggcgccatg gtggggcatg tattagcaaa ttttaaatta
     4141 gaagaaatga aattgatcca agcacaattg tatgttttgg gtacatcttc gcaagaatta
     4201 agtacccttt tttcagaata tgctgatcaa gaagcgagat ggttcaataa aatctctcat
     4261 gagttagtga ttgaaaatga aatcgttccg tctactttag aagaagtcat gcgttatgga
     4321 aaaatttacg aacatggtca aaacaaatat ttaccagcct caacaatgtt ggaaaacttt
     4381 gcaaaagatt ttgatttcca aaatgtcttt atcacacggg caatcaaatt agctgaaaaa
     4441 gaagaaaaat atggcttgca acaattgctt gttgaacttt taagtttcaa taaagaaatc
     4501 atctaccaag tacaaagact ccaaggaaaa actgtccgac aagatttgga tgaggaagac
     4561 gatgatgact aaaagtctat gtttcttcaa atacaaatat aaagcgtgag gtaaattgga
     4621 aaatcacgaa gattaaaata aaagaagcac ccgctgatgt attacagaca acatcagcgg
     4681 gtgcttctca gtttttagaa gatttgaaag attgatgaga aaatacgaaa aatcaataaa
     4741 atcacattgc ttatttgata gaataaagaa gtgggagtaa tcgaaaggag ttttttaatt
     4801 gaaaaaaagg attttagtaa caattggtct agcttttttg ttagctggtt gtaccaacgg
     4861 gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa gcaacagttg caactactac
     4921 aacaactagc acagagagca agtcaactgc agcaagtaca gaaacagcag atacctctac
     4981 ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca tctacttcaa gttcttctgt
     5041 agaggcttca tcttcctctt cagaaagcag cgaacaagtt gaacaaacac tgtggaatgc
     5101 agacaaatcc gcacaattag aggcatttat gaccaaatgg ggccaaacaa tgggacaaca
     5161 atacaaaagt tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagc
     5221 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     5281 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     5341 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     5401 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttca acgagacagc
     5461 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat ggacaagcaa attaatgaga
     5521 taaacaaggg gttgtgacat gtcttttatc acaacccctt gctcagttta tttgttagtc
     5581 ttttattttt tgtgtcgccc atgttgcact gtactggtta cctgcaacaa gatcatattg
     5641 tatcagacga taatcttcta gaatttcttt ttgtttctca gttaaatttt tttcttggat
     5701 aatttgacta tcctctgtaa caaagacctg ttttccatta aaacgattag acaagctttc
     5761 tgtaggatca attgttaatg caggtagatg attagatact tctgtcaaca acgcatagta
     5821 aggagttact ttgatattcg catgctcaag agccatagca ccaaagttat taggagcaac
     5881 aacttcttta tttaacgtag ctgcttgttc acgactatac ttgttgttat aaatgaaata
     5941 atctgtctgg tgctgttcta atccatattt tttcgaatca ttcccactat agattgcggg
     6001 taaatggtca ccataaaaaa caaaggtaat cggtttatcg attttgtcga tttcttggat
     6061 gaattttttt actgctgtat cggtatagtg aaggccttgc atataagttt gtaattcttt
     6121 tcttctatta ggaatgacag cagttccttc agcagtgtag ttcaattcac tatagtaatc
     6181 atcataaggc atatggtttt gcatagtgga taattggatg aattgtgacg tttccgtatt
     6241 gctttgtaaa gctttcaagg tttctttgta tgctgaatca tccgatatgc gtgggctttg
     6301 ttctatttta tccgtataag ttaatttatc cggactgtct aaataataga acctgtcaaa
     6361 accaaattta tcaaacactt gttttctgcg ataaagagca gctgtaaaag gatggatggc
     6421 tattttttca tcgaatagat cggtaatatt tggacttatt ttctgacgct ccactagctg
     6481 ggtataaggt gtaactaacg aagcagacag gttactgata ctcaagcctg tcagtccttc
     6541 ccattccatg ttagctgttc cgccaccata accgacggat aacatcaatc ctcctgttcc
     6601 ttctgttata atgcttttga tgtaaggaat agggtctgta ggtactgtca gattagggac
     6661 acgtgcagga tcactgaagc tttcacttaa attgagaacg atcgtctgat tgtttaacca
     6721 gtcacttctt gtttggttga tttcatctgc ctttgtgttg tactttttca ttattccatt
     6781 aattttttct ttcgaatagc cagcaggttc ttccatgatt ttaatgtcga tattgtttag
     6841 gaattgaatg actggtccgt tatcttttac ggcgccttgc tggttaaaga aagtcttgtt
     6901 tactttaaat aaattaaaca gcaagaaaga aggcgaattt ttgtgattaa tgaaaaagac
     6961 acccgaaaaa catcctaata atacagcgat actgataaat cgacgtttcc aatgaatctt
     7021 caaggaatac tgtttttgta gtctacgttg tacaataata ctagtaatca ataataggag
     7081 aaggatcaat ccgcccacga ttaataagac tgggctaacc atcgataata gttctgacaa
     7141 tccggtcagc atttttaaat ctgaaggaaa gacaggctct tcccgcattt ccattttgat
     7201 tactgtcgaa atagtgagca gtaagttaat gatcaaggta gcagtcaagg cgtaccaaaa
     7261 acgattggaa atcaacaata gtaagaagaa aaatcccata ataatgatga ttgtcaggat
     7321 catgggtgct tgtcgctgag taaatatttt taaggcaacc tggatagttt ctttccaacc
     7381 ttcttttggt tctaaaacga aaatctgcac gaaggtaaag cagtaaaaaa atgctgcaac
     7441 taaaaaataa cttttatgaa caaaaagcca atgaattctt tttttccaac gctgagttaa
     7501 ttgttcaatc gaatcaaaag cgaagtattc gatgaaacga tgaatgaaac ggattttttg
     7561 tatataaaat aaaaagataa ctaggaggga cactgctaat aaatatcaaa taaaaaactg
     7621 cccgatattt atcagccatg ccttccctga gtttggaaat tcacgtttca taaatgtaga
     7681 tacgcgataa gaaactaaag ggaaattaca tataacttga gtgacaatca aaaatacagc
     7741 aagaaaattt cctcttcctt ttatacctaa tttatcagat agcatttcta aaaataaaaa
     7801 cgaagagaaa gtataataca ttgcgaagag agaatatggt ttagaaaacc tcagcgctgt
     7861 atctgtattt cctctgatga tgagtgaaat ttgtgtcatc aaaataatca aaccgaataa
     7921 aacaatcaag ctgataaata cttgaggaag cttatagcgg accttttctt tccagttcca
     7981 ttctttcaaa ccataaccca ttaaaaataa ataaaaaatc cataaaatac tctgtccatt
     8041 ctgtaatgca aacgggtctt tattaaatag agtagagcag ccaactaatc caattgttaa
     8101 aaataatagt agatatttaa ttgttgtttt cgataattta tttaaaaatc caactgtata
     8161 aggaatacta caaaaaagca aagtacaaga aacagcataa gtaaagtaat tctgactgat
     8221 aggtgttagg ataagccaat aatcacgaat attcaagctg ccagcttgta cgtaaaaaaa
     8281 gatattgcaa actagtgtgg ctattaggaa tataaaggca aaacgactta aataatgtaa
     8341 aacttttaaa cgggcgattt tttgatattg gtaacccatg taaagtggga tcaaatttgc
     8401 accaataaca acggaagttt gtaaaaatgt atagatgtag aatcgaccag ggtttgttgc
     8461 atatgtagga atagtgactg ctgaaatcaa tatacgctgt aaaaccatca ttatacaagc
     8521 gacaaagatt gtcaggaagc cataactctc cttctttatt tttttcaaga ataaacacct
     8581 cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg ataagttgcc cattaataac
     8641 atcctaactt agaatatctc aaaaaacaac gtgtagacag aaaaagtaca aaattagcaa
     8701 gaaatggttg tttgtattgt tagaattttt agaaaattat acctttagtt tctttgcata
     8761 aaatgataca atgattgtta aaattgtgaa agggtggtca tttgttagag aagtttttgg
     8821 atatgtcgtt agttcaacgt attgttgttg gtattttagt cggaatattg tttggtatct
     8881 ttttccccac atggacattt atttcaatat tgggaacgtt gtttgtcagt tgcttgaagg
     8941 caatcgcacc tattctggtt tttttcttga cgatggcgtc aattgccaag cataaaattg
     9001 ggaataagac attcgtgaag cctattttga tcttatattt agtgggaaca ttgctttctg
     9061 cacttgtagc agttattgta agttcaattt ttactgttcc tattacttta caagaaacag
     9121 tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc tactatgttg acgaatgtca
     9181 cacaaaatcc tgttcaatct cttattgatg cgaattacct tggcgtctta ttctgggctg
     9241 tattgttggg actagctttt cgagcacgtt cagaatcgac aaaagagttg gtggatcaga
     9301 tatctatagc gttgtcacaa gtcgtacaag tgattattgc atttgctccg atagggattt
     9361 taggattggt ttatcaatca attgctacga ctggaatcgc tggattggca gagtatctgc
     9421 agcttttact tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg tacccattcc
     9481 tcacgttttt gtttattaaa gaaaatccct atccattgat tttcttttgt ttgaaagaaa
     9541 gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa tatcccgata aacatgatgt
     9601 tggctgaaag attgaaattg acaaaagagt cttattccat atcgatcccg ttaggtgcta
     9661 cgatcaatat gggcggtgcc gcgattacca tttccttgat gacgttgact gctgtacatt
     9721 ctttagggat tgaagcacct tttgttttga aagtcctttt atgtattttg tctgctttag
     9781 ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt attgattcca ttagcatgta
     9841 gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt cggtattgga tttattatcg
     9901 gtgttatcca ggattcaatg gaaactgcct taaattcgtc aagtgatctg ttgtttactg
     9961 caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg aaagattgtt
    10021 tagatggatt aagtgaacgt tgaacgttag atatttagta tttttaaaat gattcgagtt
    10081 tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca agataaagtc
    10141 gtttataata acaattaaaa gcataggtaa ttgttattgg aggcgtatca tggatttttt
    10201 acgtaaattt ggttttttct ttttattatt aggcgtgctg agtgattttt tgactccgta
    10261 cgtattagga ttgtattatc ctgaattaga tcaaatgaag agcgtgatca gtgtatttgg
    10321 agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt cagggatctt
    10381 tttcatcctg tcattgcctg cattgtatgc tgcatttgtt agtacatctc gtgtgttagc
    10441 taccctagtt acagcagcca tcgcgttcta tggtataggt gattgtatct ttacaggttt
    10501 gttcagtatc aatacccatg aatcttcgtg gaatgtctcg acatgggtcc ataatatcgg
    10561 atcaggttta ggttacagtg gttttttatt gtttccagtt cttcttttct tgatctatcg
    10621 aaaaaatggg caaatggacg taagccactt ataccttgga ctgtctgtga tcagtttgct
    10681 ttttgcggcg atttatggac tggcaagaat tccttctata aattcttggc cattactgaa
    10741 taaaataggt ttttgccaaa gaatcagttt tttctttaat tatgtgccca ttgcctggtt
    10801 gagtatcttg catctcaaac agtaaaactt gagccgtcaa attgaacagt cagcggatac
    10861 ttcatccggt ggctgttttt tgtttttctg aaatatatat ttccacagtt ttcttgtata
    10921 gcttctgtct tagagaagca aggagctata ttttgtgcta caatcttaaa aaggtagaga
    10981 gaagaccaaa tggaagacag aggagccact aaaatatgag aaacatcaag ttgacaatcg
    11041 aatatgacgg aaaaagatat tcaggatggc aaagacttgg agatgacgat aaaaccattc
    11101 aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga agagatagaa attattggat
    11161 caggaagaac agatgctggc acccatgcgc gaggacaagt agcaaatttt aaaacaaaca
    11221 cagaaattgg actggcagaa atgatcgact ttatgaatcg ttaccttccg cgggatattg
    11281 tcatcaaaag aatcgaagaa atgcctgagc gcttccattc aagatataat gcggtgggca
    11341 aaaaatacag ctattatgtt tggaacaacg tgattccttc agcatttgag cgaaaccaca
    11401 gcttttattt ccctcaagag ttggatatgg acaagctgaa tgcagcttgc gaaaaattag
    11461 tgggtacaca tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa tccacagtgc
    11521 gtacgattga tgaattatct atcgaacgaa agggagaaat gcttcatttt acttttgtcg
    11581 gagacggatt cttatataaa atggtgcgga ttatcatggg gacattacta gaaatcggca
    11641 caggtgccat gccgatagaa acaatcgatg aaatatttga aagcaaagtg cggcaacatg
    11701 caggagaaac cgtccctgca caaggtctgt ttttggatga agtttattat cgataaaaat
    11761 actttgataa tggatgaaac gcatgtttca cagtataaat atcgatgtta aataatgtat
    11821 gaagcaaatt aaagaaatat aaaaaaagac tttttagcct gtttatgtgt acagctaaaa
    11881 agtctttctt aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg ttgtttttgt
    11941 ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag taacgataga taaatatctt
    12001 gaagcaattg ctagttttgt gtactgataa gtaataacga ctttatttat acctggagga
    12061 atagttaatt cagttgtgcc aaacttagat aattttgttt gtactagttt gccattaata
    12121 gaagccactt gccctttata acgataaata ggtgtcgtca agacagtatt tttattattc
    12181 gaattatcta attcaattgt aagtttgtta ctcataaaat gagttgttgg atttatttcc
    12241 tggttaccta gtaaatattg atcattatta atcatatcag gatgatagat tgactctttg
    12301 tttgcataat cagtatgatt gtaattttca gctatagcaa cagcgtcttc aggtgctata
    12361 agtgttaatg aatctttgtt agcatggata gaattgctta ctcctgagag atgcaatatt
    12421 agtgcaagaa acgtaaagac cattattttc catgaattat ttttagtaga aaaaatataa
    12481 tagatcacta tactcatcgt ataggctata aataaagaag aaaaaccatt taaccgccag
    12541 acaaattgta gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt
    12601 gaacaaaaaa gaacaaatac accaagaaag aaaataaata aatcatcagc agttaatttt
    12661 tttataaaaa taaaagcacc aatcaatccg agaaataaga ataatccaat agtatagttg
    12721 ttatagttat tatttaaaat atgtgtcagc atatcactag gagccatacc acttaattct
    12781 tttcccagtg ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat
    12841 cccgcactta aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt
    12901 ttttggtccc aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata
    12961 gcgacagaaa gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat
    13021 ttttggtaat ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt
    13081 aaagcgacag cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa
    13141 aatgtataaa taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata
    13201 gaaaaataag aaacgaccat tgttaaaaaa gtgataaaaa aatagtagat attataactt
    13261 aaaacaagat ttcccatcat tttataaaat aaaaaagctg gataaagagt aagccatgga
    13321 taaaaaatat tcatcattgt tccatgatga tcgaagttat tgaagtttac tggagaactc
    13381 catacattat ctagtcctat gattcgagat aaatgaaagt aagtatcctg attgacacta
    13441 tgatagattc ccttagacat gaatagaggg atgacgtaag caaaacttag aaataacata
    13501 aaa
//
LOCUS       DADVXO010000078        13407 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.080, whole genome shotgun sequence.
ACCESSION   DADVXO010000078 DADVXO010000000
VERSION     DADVXO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.080"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            155..949
                     /locus_tag="KB950_001900"
     CDS             155..949
                     /locus_tag="KB950_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858117.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            939..2981
                     /locus_tag="KB950_001901"
     CDS             939..2981
                     /locus_tag="KB950_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858118.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3262..3411
                     /locus_tag="KB950_001902"
     CDS             3262..3411
                     /locus_tag="KB950_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBB6858119.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3422..4936
                     /gene="dltA"
                     /locus_tag="KB950_001903"
     CDS             3422..4936
                     /gene="dltA"
                     /locus_tag="KB950_001903"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBB6858120.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4933..6138
                     /gene="dltB"
                     /locus_tag="KB950_001904"
     CDS             4933..6138
                     /gene="dltB"
                     /locus_tag="KB950_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBB6858121.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6183..6416
                     /gene="dltC"
                     /locus_tag="KB950_001905"
     CDS             6183..6416
                     /gene="dltC"
                     /locus_tag="KB950_001905"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBB6858122.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            6419..7687
                     /gene="dltD"
                     /locus_tag="KB950_001906"
     CDS             6419..7687
                     /gene="dltD"
                     /locus_tag="KB950_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBB6858123.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7828..8910
                     /locus_tag="KB950_001907"
     CDS             7828..8910
                     /locus_tag="KB950_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBB6858124.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8910..9713
                     /locus_tag="KB950_001908"
     CDS             8910..9713
                     /locus_tag="KB950_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBB6858125.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9783..10523
                     /locus_tag="KB950_001909"
     CDS             9783..10523
                     /locus_tag="KB950_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6858126.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10534..11538
                     /locus_tag="KB950_001910"
     CDS             10534..11538
                     /locus_tag="KB950_001910"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBB6858127.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(11583..13346)
                     /locus_tag="KB950_001911"
     CDS             complement(11583..13346)
                     /locus_tag="KB950_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858128.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4311 a         2196 c         2704 g         4196 t
ORIGIN      
        1 aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg ctgaataaaa
       61 taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata agagcaaatt
      121 attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag gaggagaaga
      181 attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca aactttctcc
      241 cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg ttgggattat
      301 gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga tcaatttgcc
      361 tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa acagtgagct
      421 cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt tgatcgatac
      481 cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga ctaaagagga
      541 aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac tgctcaaacg
      601 gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta gagcacttgt
      661 tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact cgaagtcggc
      721 tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa cgatactgtt
      781 agtcacccat gatccatata cagcaagcta ctgcgataga attttattta ttaaagatgg
      841 tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg agaaagtaat
      901 agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa ctagccgtta
      961 aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg actttatccg
     1021 taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc agaagagcaa
     1081 gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt actgttgttt
     1141 tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa aaggaaatag
     1201 gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat gtgttagaaa
     1261 tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt attttttcca
     1321 aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt ccatttttta
     1381 tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt ttagcagtat
     1441 ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt ggagaaaaag
     1501 aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc gcagtcatcg
     1561 ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa atcacttcat
     1621 ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt ttaatttttt
     1681 tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta tttttgatca
     1741 tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg ggtaacacgc
     1801 aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt ctcggaattt
     1861 ctttaatgac aattggcggc acggctagta tctctactat tttatcctac caggagaaag
     1921 tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc aagccgattc
     1981 tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa gtcattggga
     2041 gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg atcacggaaa
     2101 cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct ttgaaatcac
     2161 ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt tcattatatg
     2221 gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg cttccaagtg
     2281 tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat gataccgttt
     2341 ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca ggtggaaata
     2401 atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc aatgctgttt
     2461 attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca aagaaacttc
     2521 cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg aatttctctt
     2581 ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt tttgtcacac
     2641 tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc cggcaattag
     2701 cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg attccacaaa
     2761 gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca cccatgatat
     2821 tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata aaaactgccg
     2881 attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt ttgctttatg
     2941 tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa taagacaaga
     3001 taataaatag aactaaattt tgaataccat ttatttggaa taagaggttg tgacacgatt
     3061 tttgtcacaa cctctttatt ttttatattt tttaaaagta cgagaaatta taaagaaaaa
     3121 agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt agtaaaaaaa
     3181 cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac tttgtgtgag
     3241 gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt gaattattgg
     3301 ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct gatttatctg
     3361 tatcactata aaaatattca aggaggaact tttatctata acgaatttta ggaggaagag
     3421 tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg aacctgatcg
     3481 agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag catcagacgc
     3541 acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg tttttggcaa
     3601 tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc atgcctattt
     3661 gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg tggctaaacc
     3721 atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc ctgtcataac
     3781 aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca gaaatagcgt
     3841 aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag aaccaaaagg
     3901 cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg aagactttgg
     3961 gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt tatcggtatt
     4021 ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata aagcagttgt
     4081 tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg tgtgggtttc
     4141 tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag aaaatgtgcc
     4201 ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg cagaaagtct
     4261 acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag aagcaactgt
     4321 agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc gactaccgat
     4381 tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac ctgtttcaaa
     4441 aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt acctgaacaa
     4501 tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt accatacagg
     4561 ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta tggatcagca
     4621 ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc tgctccaaga
     4681 caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag tacagcagct
     4741 ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt tgactaaatc
     4801 tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga agttccatta
     4861 tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat taacagctga
     4921 ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt ttattttttt
     4981 cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga aaaaagatta
     5041 ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg aggtaattat
     5101 tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact tgtatggggc
     5161 tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc cgttatttta
     5221 gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg aaaagcatcg
     5281 ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat gatcatggaa
     5341 ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt tctgctgttc
     5401 tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa agatcagctc
     5461 catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca aaatattttt
     5521 gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact gcttcctgta
     5581 gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg ttatatgtac
     5641 gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc agtcggtaca
     5701 agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt atcatggaat
     5761 atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg tgattatgta
     5821 tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa gatcgtaatg
     5881 tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg attgacgtgg
     5941 tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga tgcatggatc
     6001 cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca tggcttagcc
     6061 gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg atttttagat
     6121 aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag gagaaaagga
     6181 aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat gaagtaagaa
     6241 aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca tctgttcaat
     6301 tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa ttcgagcgag
     6361 aggattggtg cacaccgaat aaaatcattc aacaagcaac tgcattgaag ggttgagtat
     6421 gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct tactgtttgt
     6481 gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta gacgtgcctc
     6541 ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg cattatctga
     6601 aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc catttcatcc
     6661 gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg gagcacccgg
     6721 gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta tgaaaaacaa
     6781 gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa aaactgatta
     6841 ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa tgaagaaagt
     6901 aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag taaaagagaa
     6961 tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt tacctgaatc
     7021 tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag tattcagcaa
     7081 tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat taccaaaaca
     7141 ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag aaacgacgaa
     7201 taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc acgagccaga
     7261 gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt ctgattttca
     7321 gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca tcccaccggt
     7381 taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag gatttgccaa
     7441 aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt ttgtcaatca
     7501 agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag gctggttggc
     7561 tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta gtaagtatca
     7621 tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata aactacaatt
     7681 aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga atattctgaa
     7741 taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt ataatcacaa
     7801 tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct acttattgat
     7861 ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt attcagagaa
     7921 tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt gattgccaaa
     7981 catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga agttggtttt
     8041 atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg aggttggtgg
     8101 gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa aatcgtccac
     8161 ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa taaaccttat
     8221 gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc caaagagtgg
     8281 ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat gaatggcagt
     8341 aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc tctgcgagta
     8401 ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag cgatgtgcaa
     8461 gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc cgatattgct
     8521 tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa agaagcagat
     8581 tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat cccgcataaa
     8641 aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt ccaatataca
     8701 gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga tggtgttccg
     8761 tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt gattcatgaa
     8821 gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct tgatagtgaa
     8881 acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt tctgaatatc
     8941 ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg cttgttttct
     9001 tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt ccttttccgg
     9061 aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt ttattcgcat
     9121 tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga ccaaaaagta
     9181 accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc atcaatttga
     9241 tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg tttttaatca
     9301 gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct acgggtgtca
     9361 tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg atttttgttt
     9421 cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg acaaagttcg
     9481 actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta ttgaaagata
     9541 gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg ctaggagcaa
     9601 tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca gttgcagaat
     9661 tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact tagcatgtaa
     9721 gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag ggggaatgta
     9781 cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat caagggacaa
     9841 caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt agtgctacgc
     9901 tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc cttagccatt
     9961 atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa cttcatccta
    10021 agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc attcactttg
    10081 aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct gaagaattaa
    10141 aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta tgttacgcca
    10201 tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat tcaggttatt
    10261 tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat acctatcttt
    10321 ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga gaacttgcga
    10381 aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt gatttagaac
    10441 aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa cttgctgcag
    10501 ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa tgaaaaccac
    10561 gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc tttagatgaa
    10621 cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa ccaaaatacg
    10681 cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt ccgtcgctta
    10741 agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt tatcatgcct
    10801 gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat ccaagcactt
    10861 catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt cattgatggc
    10921 atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc tcatggaaca
    10981 attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa aaaagacaaa
    11041 atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa accatttctt
    11101 ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt gttgaaaatt
    11161 ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga agattggaaa
    11221 aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta cgggaagtct
    11281 ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga agtccatttg
    11341 aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt cggactagga
    11401 gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc atctctatca
    11461 aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc agaagaattg
    11521 cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga ttccgctatt
    11581 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
    11641 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
    11701 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
    11761 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
    11821 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
    11881 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
    11941 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
    12001 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
    12061 aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa atcttgcaga
    12121 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
    12181 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
    12241 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
    12301 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
    12361 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
    12421 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
    12481 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
    12541 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
    12601 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
    12661 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
    12721 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
    12781 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
    12841 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
    12901 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
    12961 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
    13021 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
    13081 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
    13141 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
    13201 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
    13261 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
    13321 taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgctcca ctttatacgt
    13381 aaataaaaag attctttttt attatat
//
LOCUS       DADVXO010000079        13264 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.081, whole genome shotgun sequence.
ACCESSION   DADVXO010000079 DADVXO010000000
VERSION     DADVXO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.081"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            5..2377
                     /locus_tag="KB950_001912"
     CDS             5..2377
                     /locus_tag="KB950_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858129.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="KB950_001913"
     CDS             2438..3898
                     /locus_tag="KB950_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBB6858130.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="KB950_001914"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="KB950_001914"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6858131.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="KB950_001915"
     CDS             6129..6278
                     /locus_tag="KB950_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858132.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="KB950_001916"
     CDS             6353..6946
                     /locus_tag="KB950_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBB6858133.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="KB950_001917"
     CDS             6959..7858
                     /locus_tag="KB950_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBB6858134.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="KB950_001918"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="KB950_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBB6858135.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="KB950_001919"
     CDS             8724..8984
                     /locus_tag="KB950_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858136.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="KB950_001920"
     CDS             9429..9635
                     /locus_tag="KB950_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBB6858137.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="KB950_001921"
     CDS             9683..9886
                     /locus_tag="KB950_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858138.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="KB950_001922"
     CDS             9902..10339
                     /locus_tag="KB950_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858139.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="KB950_001923"
     CDS             10332..11039
                     /locus_tag="KB950_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858140.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="KB950_001924"
     CDS             11419..11598
                     /locus_tag="KB950_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858141.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="KB950_001925"
     CDS             11923..12186
                     /locus_tag="KB950_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBB6858142.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="KB950_001926"
     CDS             12180..12530
                     /locus_tag="KB950_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6858143.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="KB950_001927"
     CDS             complement(12554..13168)
                     /locus_tag="KB950_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6858144.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4904 a         2194 c         2730 g         3436 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggta
//
LOCUS       DADVXO010000080        13236 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.082, whole genome shotgun sequence.
ACCESSION   DADVXO010000080 DADVXO010000000
VERSION     DADVXO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.082"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            151..1602
                     /locus_tag="KB950_001928"
     CDS             151..1602
                     /locus_tag="KB950_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBB6858145.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1951..2583
                     /locus_tag="KB950_001929"
     CDS             1951..2583
                     /locus_tag="KB950_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBB6858146.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3171..4610
                     /locus_tag="KB950_001930"
     CDS             3171..4610
                     /locus_tag="KB950_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6858147.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4905..5123
                     /locus_tag="KB950_001931"
                     /pseudo
     CDS             4905..5123
                     /locus_tag="KB950_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5372..5587
                     /locus_tag="KB950_001932"
     CDS             5372..5587
                     /locus_tag="KB950_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858148.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5804..6060)
                     /locus_tag="KB950_001933"
                     /pseudo
     CDS             complement(5804..6060)
                     /locus_tag="KB950_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6247..7158)
                     /locus_tag="KB950_001934"
     CDS             complement(6247..7158)
                     /locus_tag="KB950_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6858149.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7177..8181)
                     /locus_tag="KB950_001935"
     CDS             complement(7177..8181)
                     /locus_tag="KB950_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBB6858150.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8178..10301)
                     /locus_tag="KB950_001936"
     CDS             complement(8178..10301)
                     /locus_tag="KB950_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6858151.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10306..12753)
                     /locus_tag="KB950_001937"
     CDS             complement(10306..12753)
                     /locus_tag="KB950_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6858152.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12740..13129)
                     /locus_tag="KB950_001938"
     CDS             complement(12740..13129)
                     /locus_tag="KB950_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6858153.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
BASE COUNT         4080 a         2543 c         2443 g         4170 t
ORIGIN      
        1 attttaaagg cagtaacagg attaaacatt gaaacataat cgttactaaa tgaaatcaag
       61 aaatatactg tattactcaa ttcattgaaa atatttatca taatttttaa tagaaaacac
      121 tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca
      181 gttatattaa tgtcccaact ccttatagca acacctttcc atgtgttggc aagcgaaaat
      241 tcaacataca aaattgcaaa agaaagaacc gagacgactg aaatgagtaa ttccgatgta
      301 acagcagaga gtagtgaact tttgacaact tcatcaagtg aaagtagtac gacttcaaat
      361 gccgagtctt caacaacttc atcaagtgaa agtagtacga cttcaaatgc cgagtcttca
      421 acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac aacttcgtca
      481 agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa
      541 ataacttctt cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa ggaagcgcca
      601 aaaacggaag gacaaacaaa aactttttcg acaccacagc aaatagaatc aacagaatct
      661 ttccaatcac aaccaatact atctggtaat gaagaaattc attttgacaa aaatgaatct
      721 gtagaaaatt ttattatgaa aattggtgag tctgcgagaa aaattggtca gaaatatgat
      781 ttatatgctt ctgttatgat tgcacaagcg atcttagaat ctgccagtgg tcaaagccag
      841 ttagctcagg caccgaatta caatttattt gggataaaag gaacttataa tggaaacttt
      901 gtcattatgg taacaaacga agatttgggt aatggaacgc tttatacgac ccaatcaaaa
      961 tttagagtgt atgaaaacta tgaggaaagc tttgaagatt atgccaaatt attaactaag
     1021 ggaattagtg gaaataaaga tttttatgca ggagcattaa aagctaatag taaaacatat
     1081 cgagaagcaa ctaaattcct tacaggacgg tatgcaacag atacacaata ctatttgaag
     1141 ctaaatgaat taattaaaac ctacgattta accaactatg ataaagaagt taaaggtcca
     1201 caaatgagta agactggata ccaagtgcct attcataatt attcaataac cagtttgttt
     1261 ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg cagctacaca gggtgagccg
     1321 attcatgcag ttaaagctgg taaggttgtt aaagcagagt ttcactactc ttggggaaat
     1381 tacgtggtga tacaacacga agatggatca accgcgttat atgctcacca gcaacaatac
     1441 ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa ttggctatgt aggaagtact
     1501 gggaatagta ctggcccaca ccttcatttg gaaatttgca aagatagtag tctttctcag
     1561 agcatgttag ttgatccgaa aaccttatta ttcggagact aaaaaaggta atcaatctga
     1621 caaagtaaca agaaaatggt tggattctta gacctttgat caggatattg agactggaat
     1681 tgatatctcg attacgatgg gtttgacaaa taaaacaagt ccattatcga atatcgagtg
     1741 tattattgcc gtctttatgc tcatattttg agcaggtttc actagatgaa aacaatttat
     1801 ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta gtcataaaat
     1861 cagactaaga aaaattggaa atattttttt ctgatttatt aaaagttaat tgaaattata
     1921 tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca ccttactgtt
     1981 gttgaggaga ttactcataa tggatataaa aaatatcgtt gcctatgtga ctgtgggaat
     2041 gaaacgattg tatatcgatc aaacttacta tcaggtagaa ccaaaagttg tggatgtgga
     2101 aattataaaa ataggcataa atataaaaat ttgacaaata aaacttttgg taagttagtt
     2161 gcaatcaagc caactaaact gcgaagtgaa ggaactgttg tttgggaatg tcaatgtctg
     2221 tgtgggagaa aaaagttagt atcggcaaag cgacttctaa aaggtgatgt aaaaagctgt
     2281 ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt acttactgca
     2341 aagtatcctt tggacaataa ctgttcatgg aaaacaaaat gggtttgtca ttgtgagtgt
     2401 ggcagtatgt gtgagataag ttatagtaac ttattttttg gacatactca aagttgtgga
     2461 tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga cagtaataga gtgtctaacg
     2521 tcaaaattac cgaaaaataa tacaagtggt gttaagggag tttatcgttc taaagagaaa
     2581 tagattgcgt atataacttt aaaaaaaaag aggtactatt taggatcgta tgattctatt
     2641 aagagtgcag ctcaagctag aaaagatgct gaaaataaat tgtttcaacc tttgattgac
     2701 aaatatcgag gtaatcatta attattgtgg ataataatta acagtgatat ataaacatct
     2761 ataatatatt attcatacta ttgatattta gtataatcct atggaattga atatcatagc
     2821 aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa caccataaat atgaaaaaat
     2881 tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca ctattaatct
     2941 gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga attatcacta
     3001 gttactatag agtaagttca aataaagcat ctccaactta catggttaca tgaggatatc
     3061 aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg ggcagtagac
     3121 ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt atgtatagtt
     3181 atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct atatctatgt
     3241 ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct accatctcag
     3301 atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat ctgatgattg
     3361 tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa atcaaagtat
     3421 ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc aatcattcgc
     3481 taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg actatggcta
     3541 caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg gcattgggag
     3601 caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc ggcttgattt
     3661 tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga atcgtaacga
     3721 tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat acaatcgatg
     3781 gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca atctttttgg
     3841 ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt tcagtgattg
     3901 cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt tctttcggta
     3961 tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt ttttcttcca
     4021 acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg ttgagtccta
     4081 gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg gcgcctttaa
     4141 gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc ttgggcttat
     4201 tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg tggctataca
     4261 ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc aataatacga
     4321 tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg aattcctttg
     4381 cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta tatcaagcga
     4441 tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg gatgtctttt
     4501 tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt gcctttttcc
     4561 tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga aaaaaccact
     4621 gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga tgatggaaga
     4681 tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac tagtgctcaa
     4741 tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg caagctaggt
     4801 atcccaccga tggactacgt tttagaggct actggttatg aatacggagc caacgctctc
     4861 atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc tgtcaaaaaa
     4921 tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt agacacacag
     4981 atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt atacatcata
     5041 atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac aagcattgcc
     5101 ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa gagataccgc
     5161 tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt catgagaggc
     5221 ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct aattataata
     5281 agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta ccattttcaa
     5341 agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg atatgaattt
     5401 aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta ctggtatcaa
     5461 tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc tgaatctttt
     5521 agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga ttgaaatatg
     5581 gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga tccttggtgc
     5641 ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt tactaacaac
     5701 gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt tatctaagca
     5761 ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc agacgtgagc
     5821 ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca taatatgaaa
     5881 gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg aaattctttc
     5941 aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac aatgtttcac
     6001 tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat ctaattccat
     6061 ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata aaaaggcact
     6121 atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc tatagaggat
     6181 gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa ttagattgag
     6241 tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata ctaatttgta
     6301 ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg tactcactag
     6361 aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact ttaagtcaat
     6421 attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt cagaagctga
     6481 tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg ttttggcatc
     6541 aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg gctttccaat
     6601 cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga ttgtcactcg
     6661 atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct ccactaaaaa
     6721 tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag attgaacttc
     6781 ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac cgttttctgt
     6841 aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat ttttccatga
     6901 aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat tcactacctt
     6961 ctcttttata accacttttt catgagttgt atgttgatca atggcggtga aatttttata
     7021 aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca tttttcgatg
     7081 cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct tttgattccg
     7141 ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc cagctccgat
     7201 aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat ctcctgcatg
     7261 aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac cggcatcata
     7321 ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa tatgagtcat
     7381 ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg cataacacca
     7441 ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa acacataggc
     7501 cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt catcaaactg
     7561 agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat aattataccg
     7621 ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt gaccacctga
     7681 tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt ttttcccgac
     7741 atgatctaga tacccgccac catagttata ggcctgaata atactatctt gatcacaccc
     7801 tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt tgatggatgc
     7861 ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca taacgtcttc
     7921 cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat actctgttat
     7981 atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt cttctgagac
     8041 actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt caccaaacaa
     8101 caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta atgaaataac
     8161 acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg atggttcatt
     8221 cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt cacttgttgg
     8281 aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc ttgactagtt
     8341 agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg tgcttctttt
     8401 ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt cgcccttctt
     8461 tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat tttcgcattt
     8521 cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg gaacagtccg
     8581 cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag attgtatttc
     8641 aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc tttcaccaaa
     8701 gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg ggattgtttc
     8761 ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg atcgttcggt
     8821 aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt tgattgttcc
     8881 tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg tcccacattt
     8941 cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat gacgcgtctt
     9001 cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc gcccaatttg
     9061 aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa tggataggtt
     9121 gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac caaagtaaca
     9181 cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc taaactttca
     9241 taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa gataatgaag
     9301 agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga aatgccaata
     9361 ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt aatactgaga
     9421 ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc tttgcctttg
     9481 ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat ttcttctatg
     9541 ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga aaacaaacta
     9601 tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac aacgatctcg
     9661 gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat ttttgtgata
     9721 taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac aaaattaatc
     9781 acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc cacatacacc
     9841 cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt ctggagtcca
     9901 ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc cgtatccgaa
     9961 atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga caaatacaaa
    10021 ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct tttccctaag
    10081 ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa atctagttga
    10141 tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc atcaactagc
    10201 cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt gagtatgcta
    10261 ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact ccattctctc
    10321 ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg gatgtacctg
    10381 aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt ccaaatctcg
    10441 taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca aggttttctt
    10501 gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat tcttcaagcg
    10561 ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat tcatagcccg
    10621 cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa gaaaactcca
    10681 cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa agtccaaggc
    10741 aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg tatattcttc
    10801 aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat ttaactgcct
    10861 atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg caaaatcata
    10921 atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc cttcttttcg
    10981 aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg tccgaatggc
    11041 cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta aaaaggtcag
    11101 aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat aaggatcaag
    11161 taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat ccgcaatttc
    11221 tggcaaggtt tctttccagc caccccgttc cccttttgga tcgacaatca cagcttgtcc
    11281 accaaaaagg acggcgtaat aaactaagag attgttacta aaggactttc cgccaccgag
    11341 tgaaccgagg aatgccgctg ctagcgcatt ggtaactgaa tccttgaccc cttgtgaggc
    11401 caatgccggt ttcaggtaaa cattgcgccc cgtatctacg ttataaccaa agtaaatgcc
    11461 ttccaactcc cctaacatct gagtggcgcc aaaaccaagt ccggcgagaa aatcagacgt
    11521 aacatactgg atataatcat tcatgtatcg cttactagaa ggtaagaatt cttcatgtag
    11581 ccccatcata tcgccaaaag gccggactag ttttacattg gtatcatcat aaaaatctag
    11641 cacttcatca caccggcgct ttagttcatc tactgattcg gcacttactc gaaccacata
    11701 gctcagtttg tacatggatt ctttggactg atccaaggta gtttccaatt catctacgga
    11761 atccaatgca tctaagacat tggaatttgt ttcattgtca gattgatagg catggttgtc
    11821 caagtctttt aattcctttt tcttattgcg aacggtagca agtgcctttt tgttcgtcac
    11881 aatctccaca ttcatactag tgtcaattgg gaatgtaaat tgttgttgct ggtaatagaa
    11941 aagttctgag gagggaaact ccatttcccc cacaattgta ttaattgtta agtacgctac
    12001 atatgattca tgattctcat gttccatgca taagtaccgg ggcttttctt caatcaaaca
    12061 acgactggga cgtaataagt cgtaacgttt caccaaagtt tccgacttca gctttttctt
    12121 tggtagctga aattcatatt cttcaaatgg tgtacccttt tcgccataga tatgttcaat
    12181 caaatacatc agatcactcg gtgtgactcg tctgacttta aatcttcgag ccaatttact
    12241 ttccattagt ttttcaagct tggtatatcg gcgaatttct tcattggata aggaaacaaa
    12301 atcgcccatc aaatgatgat tcactccata gacaaattct tgaaagccgg agaagaaaga
    12361 tttcttgaga ttctttagat tgacctcttc atctgtcgca atcagtttga agccaataaa
    12421 aaagcgataa tcaatctgac tatcgccaat catggatact aatgcctctg tctgtaaatc
    12481 aattcgttgt ttcgctactt ctgttaatcg cccggtaatt tccttttttg atcgttcttg
    12541 cgtggcacga atactacttt ctgtggcaat ttgtaaggca tgaatcttgc cttcacgatt
    12601 ttgcgcaatc aattgacgaa agttatcgtg gacttgatat ttctgttcag gtgacaaaaa
    12661 ggagtaattg taaggtatta actcatagta ggcaaaacat tctccctctt gattaaagac
    12721 aagattattt tcaatatact taatcgggta catagttcac actccttacc agagtaatgg
    12781 gttcgtccca tcgtttcttc tgttctttga ctttctttcc tgcgaaagtg accttaaatc
    12841 gtaagtgata gctaataaaa gatcgtaaaa atcccaatgg tttcttaccg tcaaacgttt
    12901 tttgcgacat gaaccaagta actgccaccg gtatacctaa gtattttaaa aaggccccct
    12961 ctatcataga tagtggaggc acatgggcaa atacgatgac aaacagcaag gataaaacaa
    13021 accaagccat ctggttaaaa gtcacaggaa aaggcagttg aaagtcatta attgcataaa
    13081 tgaccttttc aactgcccaa atactggtgt agctttttat tttcttcatc aaatcattcc
    13141 tttaaaaaga aggatagttt agaaatcaac tacccttcaa taattttgtt aatatggata
    13201 ctcaaaaaca cctcgactcg ttaccaaata attgcc
//
LOCUS       DADVXO010000081        13043 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.084, whole genome shotgun sequence.
ACCESSION   DADVXO010000081 DADVXO010000000
VERSION     DADVXO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.084"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(195..809)
                     /locus_tag="KB950_001939"
     CDS             complement(195..809)
                     /locus_tag="KB950_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6858154.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1415..1792)
                     /locus_tag="KB950_001940"
     CDS             complement(1415..1792)
                     /locus_tag="KB950_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858155.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2092..2973)
                     /gene="galU"
                     /locus_tag="KB950_001941"
     CDS             complement(2092..2973)
                     /gene="galU"
                     /locus_tag="KB950_001941"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBB6858156.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3016..4035)
                     /locus_tag="KB950_001942"
     CDS             complement(3016..4035)
                     /locus_tag="KB950_001942"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBB6858157.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4668..5672)
                     /locus_tag="KB950_001943"
     CDS             complement(4668..5672)
                     /locus_tag="KB950_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6858158.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5685..6734)
                     /locus_tag="KB950_001944"
     CDS             complement(5685..6734)
                     /locus_tag="KB950_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6858159.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6751..7572)
                     /locus_tag="KB950_001945"
     CDS             complement(6751..7572)
                     /locus_tag="KB950_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6858160.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7559..8338)
                     /locus_tag="KB950_001946"
     CDS             complement(7559..8338)
                     /locus_tag="KB950_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858161.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8355..8825)
                     /locus_tag="KB950_001947"
     CDS             complement(8355..8825)
                     /locus_tag="KB950_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858162.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8837..9256)
                     /locus_tag="KB950_001948"
     CDS             complement(8837..9256)
                     /locus_tag="KB950_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBB6858163.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9337..12114)
                     /locus_tag="KB950_001949"
     CDS             complement(9337..12114)
                     /locus_tag="KB950_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6858164.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12305..12529
                     /locus_tag="KB950_001950"
     CDS             12305..12529
                     /locus_tag="KB950_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858165.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3835 a         2725 c         2125 g         4358 t
ORIGIN      
        1 ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg
       61 atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg
      121 ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt
      181 caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac
      241 aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct
      301 agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca
      361 gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca
      421 aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg
      481 attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa
      541 agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga
      601 ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca
      661 ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg
      721 atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg
      781 tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg
      841 aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca
      901 catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt
      961 gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt
     1021 caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat
     1081 cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca
     1141 gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca
     1201 agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata
     1261 aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat
     1321 aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata gtttatccat
     1381 tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc gttcttctac
     1441 tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt ctgcctatcc
     1501 gagactttct tttcatatat gggatataaa tgattagata cagtacagca aaaaataaag
     1561 caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta gcaaccccaa
     1621 cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca
     1681 tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt ccttccatca
     1741 gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc atagtgaaca
     1801 cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat cttctggctt
     1861 ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa aattaaacca
     1921 ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc gttttccacg
     1981 actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac gcacaagctg
     2041 ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca gttaactttc
     2101 tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc caatttccga
     2161 atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc ctacgtcgta
     2221 tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag tctgaagtgc
     2281 atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata tctcaaatat
     2341 tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt ttcccatttc
     2401 aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg ttttaggatc
     2461 aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa cagcggaagc
     2521 ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat ctttcatcaa
     2581 atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg cttgaagcac
     2641 ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga cttgagtcgt
     2701 ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt tttccaattc
     2761 gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta cgatcaagat
     2821 gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg gtttatccac
     2881 gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc
     2941 agcggcagga atgactgctt ttttgacctt cacttgctgt tcctcctaaa cgacgattaa
     3001 ctattgatca tctatttaac gaagagaaaa ttcattctcc atttttccgt cacgtaacat
     3061 gatttctttt gctgcttttt tgatatcttc tccattatac aaaacattat agattgtttg
     3121 agtaattggc atctcaacac ctagctgttg agctagctct actgctgctt ttgtcgttgc
     3181 cacaccctca acgaccattc ccatgttttc tagaacttct tccagcttat gtccctgtcc
     3241 taacaagtta ccggcacgcc aattgcgaga atgaacactt gtacaagtaa cgatcaagtc
     3301 ccccacacca ctcaaaccga taaatgtcag aggattggca cccatcgcga caccaagacg
     3361 gctgatttct gccaatcctc gagtcatgat tgctgctttt gcatcatctc cgaatcctaa
     3421 tccatggata gcgccggcac caattgcaat gatatttttc agtgctgcac cagtttctac
     3481 gccaatcaca tcattatttg tataaatcct gaaataatca ttcatgaaca gtttttgtac
     3541 ataggctgct gctttttgat caatacttgc agctgtaatt gttgtgatat catgtactgc
     3601 cacttcctca gcatggctag gaccagataa tacaacaatt gcctgtcttt tttcttctgg
     3661 aatttcttca gccaaaactt ctgagattcg tttatgcgta tcttgttcta gtcctttact
     3721 tgcatgtata atgattggct tagtattcat tttaccaata agttcctgtg cgactgaacg
     3781 aatcgctttt gttggaacaa caaataaaat agcatctgcg ttatccactg cttctgccaa
     3841 ttctttatat ccgataatcg attctgggat cgtcaaatca ggtaaaaaat gctgatttgt
     3901 atgatactta ttgatttcat caatctgttc aggtacattt ccccaaatac ggacatcatg
     3961 cccgttttct gccaatgttt gtgctaaagc agtgccccaa gatccaggac caagaactgc
     4021 gattttttgt ttcatggata ttcctccaaa taaataattg ataatttcca ttatagtata
     4081 catttcttaa agttgggact aatttccatt gtttttaagt gtttgtttac ttcttttatc
     4141 gtttttactt gaaataatca atgatatgtt acaatagtct acattttatt tttacactcg
     4201 ttatgctttg cttgttccta agaggaagca gttttgacaa tgttactacc agcattacta
     4261 ttaggaggtt aggaaacttc tcctagtgag tattgattta aaatacataa gagacagatt
     4321 gaacatttct aaatctgtca ggtcctgcaa cggacagatt gtctctgttg acggttgaca
     4381 taggaacttt tgttttagtc aacattctgg atacaaaacg ctatgtaaac tggcgcaact
     4441 cgttgtactt ggacttttac tacggatcca tgcaacaact aaatagtact attgcataac
     4501 aaaaaatggt tggcacccac atgtcccatt aaacagatta taagttgaac tgtagataat
     4561 gcaaaaaatg tgttttataa cgtaattgat gccaatattt taaaaaaata aagggagcag
     4621 ttatcgctgc tctctttatt ttttatattc tttttatcca gtctatccta ataatcaaac
     4681 tgacgatagt agcggcgtat ctctaatgga tggcagttca actgctccac atgagcatcg
     4741 atgcgttgtg tcactacatg catcaataag aaagacaaaa cacgtccgcg atatttttca
     4801 ctgatacctt ctactggata gtcttttgtg tctatcaatg tataattgct gcaaattttt
     4861 ggcaaaagtt tagccacacg ttcagataac actctctgtt catcttcgcc taaaaataat
     4921 gtgactgggg ttgtttcttc tacgatctct aacgtcccgt gaagaaactc ggcagcatgg
     4981 atagatttcg atggaagcca gctttgttct tcccagtaac acatggcata tgaataaaca
     5041 gctccccatt gattccctgc tccaataaaa taatgcatac tatcacgtcg atgtttttct
     5101 gcaaatgata agccaaatgc atctgctttt ttctctgctt ccactaaccc tgttggtaaa
     5161 taacgttcga gctgttcata gtattctgaa taagcttcga attctccatt caaatacatc
     5221 aatcgatctg ctgccataaa aaatttgatt tgttctgttc caggagctgg ataagtgacc
     5281 acaaaatcgc atagctgaga caacttactg ttcgctttat cgataaatcc aatcagtgtc
     5341 gctcctactt tcttgatttg ttctactgct ttcactactt cttgagttgt accggtaaca
     5401 gaggacaaaa taacgataga atcttttgtt aatcttttgt ttccagtagt gtaatattct
     5461 gcagcatgct gtacaaagac aggcaagttg cttttcccat tcatataagt gacagcttgc
     5521 atcgaggaag catacgttcc accgattcct agataataga cagcgtcaaa tttttccgca
     5581 taaagtttat cgatgatttc ttctacttgc ggacgtaaag ctagtgcgcc ttttattgct
     5641 ttgatttgtt cttcttcatt gaatttcagc atgatttcca cctcttataa tttacttttc
     5701 aagactgtat caaagtcttc aaaaatcttt ttgttcaagt caataccttt agctactgcg
     5761 gttttataag cgaatagttg aaacggaacg acaagcgcta atacagacag atattcatca
     5821 cattctattg ctaatccaag tgttttctcg tttcttgctt tttttgtggt aatggtgaga
     5881 gtctctccaa cgtaccgact catatatctt tgcaactttt gcgaacgttc cttatttaca
     5941 ctttcccctt caataaagaa tagcaaatgc gaagcatctg cctccaggta tgggccgtgc
     6001 atataagctt ctaactcaaa tccttgtgac ggaacgcgga ttgtttcagt caatttcgtc
     6061 tctgcttctt tagctgttcc ccagtttgga ccatatccaa tggtgacgaa acgctgtgct
     6121 aaacgaaata aactttgata tccatcaaaa aattcttccg ttttttgaat aatcgctggt
     6181 aaatggttga ttattttgcg caactgttgc atgtagtctt gttctatttt tttgctgatt
     6241 ttatttttac ttattccaat tcctatccct aagagtagta actgtaaaac agtcgcagaa
     6301 tagccttttg taacgaagcc tactgattct accccggcac ctaaatcaat aatttgtgca
     6361 gcttctaaag caagtggact ttgcacgtca tttgtgatag caacaacagg taacccttgt
     6421 ttttgtatca tttttactgc ttcaacagta gaagcacttt tcccgctttg tgacacagca
     6481 atcacagtat cgattgaagg agaaagtttt ccataattga aaaaataaaa aggttcttga
     6541 atatcgacag taagatcacc ataagattcc aatgctggct tcgctgctaa acaagcgtta
     6601 taagatgaac ccgttgctaa gatcagtaaa ttcactgttt ttttcatatg ttctcttgtc
     6661 gaaaaatcat tctttttcaa gatcgtactt agtacttcct tttcttcata gatataatct
     6721 tgcatcgtaa acattttctc tgctcctttt ttaaaaaatc cctatccacg aaccaaaaat
     6781 tccaaagaaa gctagtccga tcaagatcgt taatggtttc acttctttct tcaatagata
     6841 aaacactatg ccaaatgctc caagacataa gatatttgga acgatatcat caaaaatact
     6901 ttgaacagtc acagcatttt ctcctgaacc gatttttaaa ggaatattga tggtgatcat
     6961 cgtggcagtc attgctccaa cgaccatcag tccaataata gacgcgccta atgtcaaact
     7021 ttccatcata ccgtttgcct ggatcttttt caaaaaaccc gttcctaagc gatatcccca
     7081 acctgttgct agatagcgaa acagaatgtg cggaatattg aaaatcaaaa tgaacaagat
     7141 agggcctaaa atatttcctt gcatggctaa agaggtaccc acacctgtgg caatcaatcg
     7201 caaagttccc caaaagaaag aatctcctaa ccctgcaaga ggtcccatca aagatgtttt
     7261 gatactatcg atcgcagtca catcaaattc aggatcatcc ttattttctt cctccattgc
     7321 agccgtgatt cctaaaatca gtgtaacgat atgtggggtc gtgttaaaaa actctagatg
     7381 ccgtttcaaa gctgctgcca tttcttcttt cgactgatat aactttttca aaatcggaat
     7441 cattgcataa acataagcca tattcatctg acgttcataa ttccaagaaa actccatttg
     7501 aaaagaacgc caaaatacgt gatttagctc ttttttcgtt attttgctgt ttttgatctc
     7561 agaattcttc atcttcttct acctcccctg cagttgtttg aagtggtagt tgacggacat
     7621 tcataatatt gactaccaca actgctaaaa tcgcaccaaa aatagctact ccggtcacag
     7681 gaatctctaa gtaagaagct atagcaaaac ctaagaaaaa atatggtgca acttgtttgt
     7741 tgatcagcaa acgagcaagc atcgcaaaac caagagctgg aatcaaccct gtagctactg
     7801 acaacccctt ttgaacgaag tcaggaatca tatttaatag atttccgatc gtattgcttc
     7861 ccaccatata agaaaccatt accacaacac caagcatcaa ggaaagaccg ataccggcaa
     7921 gcaagtgcat atgctcgact cctttataat ttccttcttc tgcataattg tctgctttat
     7981 ggttcataat tggaatcaaa agccctaagt aaatattttt caatatcaat gtcaacgtag
     8041 caatcggcaa acctagtaaa agcgcggttt ctgtaccggc tccagctgta atagcaaaag
     8101 ccgtccctaa aatcccaccg gtcacaacat caggtggaat cgaagcacct actgagaacg
     8161 atccgataaa tgctaactct aaagttgcgc ccataatgat cccagttttt acatcaccta
     8221 acacgattcc tgtaaacagc cccgtcacaa tcggacgaga aagcaacgat gtccctagtg
     8281 catattctga ttgtgcgaca aatgcaacaa ttcctaataa gactgcttgt acaatcattg
     8341 aggttcttcc tttcttacaa aacttctctt acatccactt ttttatctgc tggtacttga
     8401 cggatttcta cttctatttg tttgtcaatt aatcgtttca agcgttcctc ctcttctggt
     8461 aaaatattta ccgcttttcc gatgctgcga gtaccaggtt ttgccttgat ccctcctaag
     8521 ttgatggttt tgatttctgg atacgcttct gccagcttat atgcatcctc gatcgattcc
     8581 actacgatga ataacttata tttatctgtc accccgcttt tcaacgcggc aatcgagtca
     8641 tcaattgatt tgatcactag tttgactcct tgtggcttgg ctaatttgat cgttgtcttt
     8701 cgtatctcat tcgtaggtac atcatcatta gcaatcaaaa tacagtctgc gcctattgtt
     8761 tgtgtccatg aaaaagctac ttgtccgtgt aataaccgat ggtcgactct tgtcaataca
     8821 atcatttttc ttcctcctaa aattcgtctt cttctatttt ctgattgagc cgctcgttac
     8881 aaaactggat catttttttt gaatcagtta gcacctgctg gatcaaaaca gttgtctcta
     8941 cttcagcgtc caaccgagat actagctcaa tcaatagtgg caaattcaat ccggcaacga
     9001 gatagacatg atcccgattg atatattgta agaattcgtt gttgacactt ccgccaaaaa
     9061 gatccgtcag aacaatgatt tctttatctt tgtacctcat aaaaagctgc cttactgttt
     9121 ccgctaaatc aaaattctca tcagtatagc aatcgaatac agtaatagaa tgatttttcc
     9181 catagatcag ctctaatgaa tctaatatcc ctgaagcaaa ttttccatgg gaagctaaga
     9241 taatttctct atccactttt tcatctcctc ttttcactta cacatactaa aaaagcaagt
     9301 tgcatgccaa aattagtggc aacaacttgc ttatgttcaa aattcttctt ctatcgagag
     9361 aaactctgta tcttcactca aaatatcata gatataggca atctcagaat tggggatcat
     9421 gacactataa tgctcttcaa tgacactaaa cgcagattta atagcagata acccttcttt
     9481 ttgacactgc ttccatttct ctaatccttg ataggtttcg attggcattt gtcggatcaa
     9541 tcgttcaatg agacagctca catggacata caacgctaat tttttgggat tgctgatgct
     9601 tcgattcaac tgcacctcta attctctcat aaaatgatcg atttctccca tcaccttctc
     9661 tgtatctaga atagtcacgg aatccagtac tttttccaat gaaaaattac gaatgatgcg
     9721 ctgattgaat atttccttct ctgaagcatt catagtagtg tccaaccatt cagctagtac
     9781 agacatcttt tcccccgaga tgaggtcttc taatgaaaga tagtcgatat tggtgatatc
     9841 aggatctgtg gtgccaatca ttcctacgac ttcgtatatg gaaaataatg ggtcactgtt
     9901 tttcttatct tttaattgct gatattcata tgggatgatt ttcaactgac aagccgtagg
     9961 taagcttttt tctagcagtt cacttaagtg gaccgccgta ccgattccag tgaagcaagt
    10021 agtaatgagt gctttgggtt tgttttcctc tggatagagc agtcgccatt ctggctggat
    10081 ctgtcgaaca gtctcttctg ctatctcttc taatgataca tttttttgca ggcattcccc
    10141 tactgtgatt gctaaaggtg tcgtcacatt attcaaaatc aaaagcggta cggataactg
    10201 ttttttaaaa aactgatgga tttctttcag agatcccatg tctactaata tgatcaatcc
    10261 atttgaaaca tcgctgtttt cgcaataatc aaggatttct gaagcaatct gctgtggtgt
    10321 gacatctagt ggcatatcaa atgattcaaa tagatcctct tgtaataaac gatttactac
    10381 atttgccata ctgctagcag ttgcataacc gtgagcagca atcatcgctt ttggcagtcg
    10441 tttttctttt gaccatttcg ctttttttag atagatcgtc agtaaaatcc gatccatact
    10501 tgtgtcttca atatctaatt tgatcttact taattccaga attcgttgga catagtgata
    10561 actagatgga taagactgag aaacttgttg ttctaattct ttgattagtt ggataatatc
    10621 atgttcttcc ggtaaccatt tgatagtgct tcgctgatag agatagtaac tcaaggcata
    10681 gacactgttt ccatcgaatt tgatttgata ggcagtttcc atttgtcgaa aggtctctcg
    10741 gatagattgt gtccaataaa ccagcagctc gtgtttttct tgtctactgg tctcaaacag
    10801 caaaaaatca aataattgaa tcacttcttg ctttaatttt ccttcgcaag aatatagcag
    10861 acagttattt ttcttgaaat caattaggat ttgctcaaaa cttttaacga ttcgctgctg
    10921 ttcgggctgt tgcttctcca ttaattgctc taaggtctgg tcttgcgtaa tatagattcc
    10981 ttgctgtgct gtatttttag taataggctg cgcaagtagt tctttttgca aatgatggat
    11041 cgtgacatgg atttcaggct gttcacgctg ttcagcatat gcttttgctg ctgaaacttt
    11101 gacgatattt ttcagctctc ctatgtttcc ggataatttc tgacttgtta aaagtgacaa
    11161 agctggtcca ctgactgaca aagggctccc catttttctt tgttcttcga gaaaaaaaga
    11221 gtaaatcagt tctagccgtt cattacgact acgctgatta agtgcaggta gttcgattcg
    11281 gataggtatt cttcgaatga aagttgttaa gaaatgacta gtaatctctt cagtcgtagc
    11341 aaaaaagaga cgtgttttga tccggcgtga caaattaggt tctcccatac gataaatcac
    11401 accttgatca agataggtga acagtttttc ctgaccttct ggtgttaagc gatgtacctc
    11461 gtctaaaaac aagatcccac catctgctgc ttcaaaagcc ccttttttat tttcttctgc
    11521 accagtgtac gctcccttaa catgtccaaa caaattgcta gtcaaaagtt caggattatc
    11581 tgcatattgt gcacaattaa aagtgatgaa tggtgcagaa tcttctagca gatcatgaag
    11641 cagacaatac tgatagacca aattaactaa ataactttta cctgtgccac tttcgcctgt
    11701 aatcaaaagt ggtaatccgc catctggata gtaaagagct gttttgattt gttcgatact
    11761 ttctttcaaa ctggcgtcgt atccgatcat aaaagagaat aagtcttttg gttgcgcgaa
    11821 aaaaggctgt tcctctttga gatcatcgat agaaaaataa aaattcgtgc gtaacttaaa
    11881 aaattgtttt tcaaaagctt ctttgtggaa ataaatcact ggtcgagaat tgattttgac
    11941 tagtagttgc tcatcattta actggttcaa ataatgactg actgtgtttc tttttacccc
    12001 aaaatgatca gctaaccttt gtgctgtaaa gacctcgttc atatgttcta aatcgaaaaa
    12061 ggctgtctgg ttctctaagt aacataaaat ttccttcttc aacaacttat tcacccctcc
    12121 tccatcatag aaaacggatt cattttgtca ataaaatctt taataaagaa ttgaaaaata
    12181 tatcttatta tagacagcag cctacttatc ccttataatt tttattctaa aaaacgtaca
    12241 gttccttcca gtttttatac tggaatttca acatatgaaa gaataaggag gctatgacta
    12301 ttcaatgaaa tcggataaat ataaaaacag gaaacatact tcctggaaaa cttttcgaga
    12361 aatcatcgga tcttatctcg ctgtagttgt atttttgcct ttgctttata tggctatgac
    12421 agaacaaact cgttttattg gcgtagatac gcaatatata gccgaaacag gatttttatt
    12481 catctttatc ttgatttttg cgttctataa atccttccat acaaaataag attcacagat
    12541 ttcaactttc ctgtctgttt ttagctatta caaatcctta tatgtggtat aacaacaatt
    12601 tattggcata atgaggaaaa acgagttatg atgctttcag agagtaaaga ggtactgttg
    12661 tcaccatcct ttttattgat tacgtacacc actcttagtt ttttcccatt gattacgaaa
    12721 acaaggtaga aacattgatg ttcctacctt gttttctttt tttattttca gatcaaaatc
    12781 tcaagtggct tttgacttga tgtcgggagg ttgtcttcgt tccatcaaac ctgatagaat
    12841 ctgcgccaaa ccatagtttt ctccactatt atttattcct ctaagatagt acccacaata
    12901 aacgaataaa tatttttctc attttcatac tccttctttg aaaatcaatt agatttctct
    12961 caacccactt ttttacaaaa attacgtagc aaaaaagatt gcaagaacaa attaaaaaag
    13021 aagtaaatag agtttcatct ata
//
LOCUS       DADVXO010000082        13027 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.085, whole genome shotgun sequence.
ACCESSION   DADVXO010000082 DADVXO010000000
VERSION     DADVXO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.085"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            410..979
                     /locus_tag="KB950_001951"
     CDS             410..979
                     /locus_tag="KB950_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBB6858166.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            966..1826
                     /locus_tag="KB950_001952"
     CDS             966..1826
                     /locus_tag="KB950_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6858167.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2016..2720
                     /locus_tag="KB950_001953"
     CDS             2016..2720
                     /locus_tag="KB950_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858168.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2725..4560
                     /gene="walK"
                     /locus_tag="KB950_001954"
     CDS             2725..4560
                     /gene="walK"
                     /locus_tag="KB950_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBB6858169.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4557..5873
                     /locus_tag="KB950_001955"
     CDS             4557..5873
                     /locus_tag="KB950_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858170.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5874..6743
                     /locus_tag="KB950_001956"
     CDS             5874..6743
                     /locus_tag="KB950_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBB6858171.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6798..7607
                     /locus_tag="KB950_001957"
     CDS             6798..7607
                     /locus_tag="KB950_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6858172.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7710..8339)
                     /locus_tag="KB950_001958"
     CDS             complement(7710..8339)
                     /locus_tag="KB950_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBB6858173.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8509..9801
                     /locus_tag="KB950_001959"
     CDS             8509..9801
                     /locus_tag="KB950_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858174.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9877..10539)
                     /locus_tag="KB950_001960"
     CDS             complement(9877..10539)
                     /locus_tag="KB950_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6858175.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10764..11048
                     /gene="groES"
                     /locus_tag="KB950_001961"
     CDS             10764..11048
                     /gene="groES"
                     /locus_tag="KB950_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBB6858176.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11099..12724
                     /gene="groL"
                     /locus_tag="KB950_001962"
     CDS             11099..12724
                     /gene="groL"
                     /locus_tag="KB950_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBB6858177.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4338 a         2235 c         2803 g         3651 t
ORIGIN      
        1 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
       61 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      121 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      181 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      241 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      301 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      361 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      421 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      481 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      541 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      601 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      661 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      721 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      781 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      841 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
      901 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
      961 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1021 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1081 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1141 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1201 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1261 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1321 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1381 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1441 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1501 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1561 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1621 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1681 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1741 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1801 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1861 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     1921 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     1981 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2041 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2101 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2161 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2221 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2281 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2341 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2401 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2461 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2521 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2581 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2641 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2701 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2761 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2821 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     2881 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     2941 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3001 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3061 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3121 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3181 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3241 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3301 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3361 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3421 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3481 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3541 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3601 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3661 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3721 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3781 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3841 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     3901 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     3961 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4021 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4081 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4141 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4201 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4261 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4321 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4381 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4441 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4501 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4561 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4621 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4681 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4741 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4801 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4861 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     4921 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     4981 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5041 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5101 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5161 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5221 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5281 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5341 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5401 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5461 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5521 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5581 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5641 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5701 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5761 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5821 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     5881 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     5941 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6001 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6061 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6121 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6181 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6241 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6301 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6361 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6421 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6481 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6541 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6601 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6661 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6721 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6781 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6841 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     6901 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     6961 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7021 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7081 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7141 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7201 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7261 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7321 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7381 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7441 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7501 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7561 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7621 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7681 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7741 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7801 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7861 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     7921 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     7981 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8041 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8101 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8161 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8221 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8281 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8341 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8401 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8461 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8521 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8581 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8641 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8701 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8761 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8821 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     8881 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     8941 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9001 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9061 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9121 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9181 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9241 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9301 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9361 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9421 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9481 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9541 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9601 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9661 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9721 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9781 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9841 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
     9901 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
     9961 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10021 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10081 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10141 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10201 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10261 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10321 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10381 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10441 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10501 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10561 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10621 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10681 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10741 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10801 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10861 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    10921 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    10981 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11041 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11101 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11161 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11221 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11281 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11341 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11401 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11461 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11521 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11581 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11641 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11701 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11761 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11821 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    11881 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    11941 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12001 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12061 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12121 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12181 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12241 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12301 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12361 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12421 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12481 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12541 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12601 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12661 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12721 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12781 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12841 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    12901 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    12961 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13021 ccattca
//
LOCUS       DADVXO010000083        12929 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.086, whole genome shotgun sequence.
ACCESSION   DADVXO010000083 DADVXO010000000
VERSION     DADVXO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.086"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(56..1561)
                     /locus_tag="KB950_001963"
     CDS             complement(56..1561)
                     /locus_tag="KB950_001963"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBB6858178.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="KB950_001964"
     CDS             complement(1653..2243)
                     /locus_tag="KB950_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6858179.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="KB950_001965"
     CDS             2422..4068
                     /locus_tag="KB950_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6858180.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="KB950_001966"
     CDS             4117..4836
                     /locus_tag="KB950_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBB6858181.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="KB950_001967"
     CDS             5315..6262
                     /locus_tag="KB950_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858182.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="KB950_001968"
     CDS             6274..7194
                     /locus_tag="KB950_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858183.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="KB950_001969"
     CDS             7235..8692
                     /locus_tag="KB950_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6858184.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="KB950_001970"
     CDS             9037..9651
                     /locus_tag="KB950_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6858185.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="KB950_001971"
     CDS             9644..11368
                     /locus_tag="KB950_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6858186.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="KB950_001972"
     CDS             11365..12816
                     /locus_tag="KB950_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858187.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         4192 a         2237 c         2592 g         3908 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgg
//
LOCUS       DADVXO010000084        12734 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.087, whole genome shotgun sequence.
ACCESSION   DADVXO010000084 DADVXO010000000
VERSION     DADVXO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.087"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="KB950_001973"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="KB950_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBB6858188.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="KB950_001974"
     CDS             complement(322..891)
                     /locus_tag="KB950_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBB6858189.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="KB950_001975"
     CDS             1077..1979
                     /locus_tag="KB950_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6858190.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="KB950_001976"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="KB950_001976"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBB6858191.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="KB950_001977"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="KB950_001977"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBB6858192.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="KB950_001978"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="KB950_001978"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBB6858193.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="KB950_001979"
     CDS             8547..8732
                     /locus_tag="KB950_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBB6858194.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="KB950_001980"
     CDS             complement(8794..9990)
                     /locus_tag="KB950_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6858195.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="KB950_001981"
     CDS             complement(10160..11596)
                     /locus_tag="KB950_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBB6858196.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="KB950_001982"
     CDS             11889..12698
                     /locus_tag="KB950_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6858197.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2673 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DADVXO010000085        12718 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.088, whole genome shotgun sequence.
ACCESSION   DADVXO010000085 DADVXO010000000
VERSION     DADVXO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.088"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(25..327)
                     /locus_tag="KB950_001983"
     CDS             complement(25..327)
                     /locus_tag="KB950_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858198.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(352..2445)
                     /locus_tag="KB950_001984"
     CDS             complement(352..2445)
                     /locus_tag="KB950_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6858199.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2934..3698)
                     /locus_tag="KB950_001985"
     CDS             complement(2934..3698)
                     /locus_tag="KB950_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBB6858200.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3809..4678)
                     /gene="mdcB"
                     /locus_tag="KB950_001986"
     CDS             complement(3809..4678)
                     /gene="mdcB"
                     /locus_tag="KB950_001986"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBB6858201.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4703..5779)
                     /locus_tag="KB950_001987"
     CDS             complement(4703..5779)
                     /locus_tag="KB950_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBB6858202.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5803..6396)
                     /locus_tag="KB950_001988"
     CDS             complement(5803..6396)
                     /locus_tag="KB950_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBB6858203.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6400..8076)
                     /gene="mdcD"
                     /locus_tag="KB950_001989"
     CDS             complement(6400..8076)
                     /gene="mdcD"
                     /locus_tag="KB950_001989"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBB6858204.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8066..8365)
                     /locus_tag="KB950_001990"
     CDS             complement(8066..8365)
                     /locus_tag="KB950_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBB6858205.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8467..10118)
                     /gene="mdcA"
                     /locus_tag="KB950_001991"
                     /pseudo
     CDS             complement(8467..10118)
                     /gene="mdcA"
                     /locus_tag="KB950_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(10125..11036)
                     /locus_tag="KB950_001992"
     CDS             complement(10125..11036)
                     /locus_tag="KB950_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB6858206.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11156..12013
                     /locus_tag="KB950_001993"
     CDS             11156..12013
                     /locus_tag="KB950_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6858207.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12200..12562)
                     /locus_tag="KB950_001994"
     CDS             complement(12200..12562)
                     /locus_tag="KB950_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBB6858208.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3583 a         2710 c         2216 g         4209 t
ORIGIN      
        1 ttttaccctc ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat
       61 tttttcttca atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg
      121 atcaactgcg aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg
      181 tttcatggaa actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt
      241 gttcaaaaca tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcaccgca
      301 tacgaataaa atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc
      361 aactactgaa aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc
      421 caacttctct tcgttgttga tcaattcaat tagttctttc atgatgttca gatgcgagta
      481 ggagtctact gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa
      541 atcgattggt tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg
      601 tcgggcatgg gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac
      661 tgcacgaatc atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct
      721 agcagctagt tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct
      781 gtccgtcaaa atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt
      841 tgtttcgaat aatttttcta acttgccata gatgattcga tctactcggc caccactttc
      901 tttcatgaca tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa
      961 ccgctgtagt tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt
     1021 taaaatcgga tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa
     1081 aaccagattg acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt
     1141 aatctcgatc tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat
     1201 cccatggtta cagacaacga ctgatttata aacataattc aagtctcgct tgattacact
     1261 ggcgatcgtc gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc
     1321 aattacttct tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt
     1381 tgttcggatg ttttctttca atggattcat gacttggata tgattcttga ttcttacaat
     1441 cagtgcagcc atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg
     1501 tacgtgtgtc tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac
     1561 ccactcgatt gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata
     1621 atttctttct acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa
     1681 tgcagaaaag ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc
     1741 atgtttcctg attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat
     1801 atcatcttct tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac
     1861 caaaggaaaa taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat
     1921 aaagaagtcg gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga
     1981 tcgttctgaa cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata
     2041 actgaccagt gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg
     2101 aaattcttct tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac
     2161 tagatcaaaa atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt
     2221 ttctaatcga gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt
     2281 acggatctcc ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg
     2341 gatcgtccgc tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg
     2401 ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt
     2461 ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa
     2521 gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa
     2581 cgaaaaagac atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg
     2641 tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt
     2701 tttcgtttgg ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag
     2761 gttgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca
     2821 tattttacaa aatcgtgcct tattgccgat aggctatctc tggcacaccg tttattcaga
     2881 aaaaggagct gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc
     2941 agcataatcg gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa
     3001 taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct
     3061 tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg
     3121 tggatctgtc gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca
     3181 ccgtctgctt tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa
     3241 cagcttctgg ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc
     3301 ttttggattt ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat
     3361 atctccgctt tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc
     3421 cctcctactg acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct
     3481 gtacctggac cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg
     3541 cccccatatg aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct
     3601 ccaccatgac cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc
     3661 actaacaaaa tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat
     3721 tacaaaaaac tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata
     3781 aactgttttt ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg
     3841 tgtcggcagt ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg
     3901 cgcttcctcc aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac
     3961 gatttggtaa agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt
     4021 tctgatcact gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca
     4081 tatgcatttg tttctgtaca tatgaatcat tatctagctc cttagctgct tgaattgcaa
     4141 gcctgatatg cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt
     4201 gcttcaccct atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag
     4261 caagcttgct gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt
     4321 tttgtgcaat gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga
     4381 tgccatttgt agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt
     4441 ctcttaactc ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag
     4501 gagttagcgt cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg
     4561 catccagtgc atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt
     4621 cctctaccaa cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga
     4681 cactcactcc ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga
     4741 tatgaagtaa ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa
     4801 tcatgactcc tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga
     4861 aaacaaatac attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg
     4921 gcaattttgg cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga
     4981 tgactaaact gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt
     5041 ctcctggatg tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta
     5101 agaaaagata acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg
     5161 ctaccccata aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat
     5221 aaggcccagt caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg
     5281 aaccaatcaa tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa
     5341 caaaaaacgg catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg
     5401 ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt
     5461 gttggactgc tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta
     5521 gttcaaaaaa cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca
     5581 tattataaac aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg
     5641 taccaagaaa agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg
     5701 tttgcatcat aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga
     5761 tttttttgtt ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt
     5821 taatttaggt ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc
     5881 ttttagtgaa aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc
     5941 aggatgcgat tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa
     6001 atcaaaatca ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct
     6061 gccgccgatt ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata
     6121 aacaggataa tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg
     6181 atgaatgact tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc
     6241 aggtccacgt aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt
     6301 tccgaacatc ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg
     6361 tttatcaaaa aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc
     6421 cattttggca cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt
     6481 gtatcgattt gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat
     6541 tgcttcgatg actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag
     6601 gaaatgatag atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac
     6661 tttttcagca gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc
     6721 tttagacatc gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg
     6781 taatccatgt gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg
     6841 atgtccagct tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat
     6901 accgatcaat tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat
     6961 tggccgtttc acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat
     7021 gcggaaacct tcaactaaac caacttcacc gtttcgtaca cggtacatcg ggtttttagg
     7081 atcaggtacg attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata
     7141 cacagtacta atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta
     7201 accaacactt gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt
     7261 tgcttgatgg ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc
     7321 taaagaatca agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg
     7381 ttcgtccatt gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc
     7441 atcaatgatt tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt
     7501 gtcactagaa tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa
     7561 accgactctc gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat
     7621 accgccaaat gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag
     7681 agcaatgatt acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa
     7741 acgaacacca cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc
     7801 ttctaacaca tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc
     7861 accttggaat tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg
     7921 gctgacaacg atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc
     7981 gatgatttga tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg
     8041 ttctctggcg tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg
     8101 tcaatcgtag gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg
     8161 aaattggata acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat
     8221 cgcttcctgt acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt
     8281 ccaaatctcc tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg
     8341 gatatttata agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac
     8401 gttctgctta ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg
     8461 ccaattttat gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc
     8521 gatcaaatca tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat
     8581 gcctagatct tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt
     8641 cgtgctagtc attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc
     8701 ttcttttgtt tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt
     8761 atcttcgcta taaatcatca ctggtgcatt gcattggcta agcctgcttg tttttgaaca
     8821 gctagtgcat ctaattcttc gacaaagact ggttttttat ttgaaccata tgtttcaacc
     8881 atttgaacaa ctaatttttg gcctttccct aatggatcat cattgtcgcg aagtgattgc
     8941 catgctggtg tagaatgacg acgaccgcca ggattatgtc ccatgttagg cgcaccgcca
     9001 aaaccagata aacgtccttt tgttacggta gaagagtttc cttcgtaatc taattgcaag
     9061 gttgaaccga taaacatatc gatggcatat tgtcctgcag cttgtgataa tgttcggtta
     9121 ctgcgcatcg tgccgtcttt accgacaaaa aagacatctg ggcgagcagc aatatatttt
     9181 tccataccaa cttcgccacc aaaactatgg atgctttcaa cccaacctga ttcgatggct
     9241 ggaatcatcg ttggatgtgg gttcaatacc cagttcttcg cgattttccc ttttaaccct
     9301 agtgattcac cataagttgg cagtaacaat tcgatagctg ctgtattgaa tccaataccg
     9361 tgattcaaag attgtacgcc gtgttctgcg tagattcctt tgattgccat cattgccatt
     9421 agaatttgca attctgtgat gttttgagga tctcgtgtga ataaagcttc taattgataa
     9481 ggtttatctg ctttcacgac aacgtctacc cagtcaccag ggatatctac acgtggtaaa
     9541 gaatcgacaa cttcgttaac ttgaacgatc acgataccat ctttgaaagc tgcagcttcg
     9601 atgattgttg gtgtttcttc cgtattgaaa cctgtgtaca agttgccgtc tttatctgct
     9661 ttatctgcag ctactaaaac aacatttgga atcaaatcga tgaataagcg tccataaagt
     9721 tccaaataag tatggatatc acctaatttc attgtgccgt cttcaatcat ttgggcgaca
     9781 cgcaagcttt gtgcaccagc ataagagaaa tcgatcttgc ttgcgattcc tttttcaaaa
     9841 atattcaaat gttctggtcg tgagatacta gacatgatca aatgcagatc attcactttt
     9901 tctgcatcta ctttttcaag actttctgaa agaaaacttg cttgtttttg attgtcgcct
     9961 tctaatacaa ctttgtcgcc gggagcaatc agtttttcca ttacttctac gattttttca
    10021 gtttctacat acttgccatt gagcaagttg ctgatttttt ccaatcgctg cgcttttttt
    10081 tcgcgtttag aagcccagtt gcggtttggt ttttccataa ttagtcatct ccatttttga
    10141 tatagaaacg tggcgtcatc aataccatgt tggtctagat tgatcgtacg gatcccttta
    10201 tctacaccaa attttgtatg aatcagcttt gtcaaagtat tgccgggggg aaattcaatc
    10261 gtcagatcca ttcctaattc ttttgccaag tccatcattt ggttccactg cacagggaaa
    10321 gctaggtttt ccaacaaatc tttacgaatc tcttctgcgt cctgagtggc tcttccatcc
    10381 gtatttttta tatacaaaca tctagcatca gatagatgga cagactgcat atacggcttg
    10441 aatttcttga ccgtatcatt catcaagaga cagtgagatg gcacagggac tttcagcagc
    10501 ttcgctgttt gcgcttggtg ccgtttggct tctgccaacg taacggataa accttcgata
    10561 ctcccagata acgtatgttg catcggacaa ttttcatttg acagataaac tggatggtca
    10621 ggatcaaatg tttttgctac aatttcctca gcttcgcttc tcgtcacacc tactactacg
    10681 cccattgcat aaccgcttgg ataagcttct ttcattaaga ctgcgcggtg atagactagt
    10741 tccgctgcat tctcaaatct caaagatcga caagcaacag ctgcacaaaa agcaccaatc
    10801 gaatgtcctg ctgcaatatc aggctgaata ccgttttttt ccatttcatc taaataaaat
    10861 actgcttttg tcagtaatgc tagctggata aaaaccgtat cttgatagtt ttcttcttta
    10921 tcaactaacc gataccccgt cacattttct aaacgttcca tgtacatttc agggatatcc
    10981 gctaacatcc ctaacttctg actgccttgt ccaggaaata agaaacctgt actcatgagc
    11041 ctcgcctcct cagcacttac tttttccact gctatcttag tcaatttctg ctataataaa
    11101 aaatgccaaa aaaagaaaaa accataacaa aaaggtatgg atgggatgga taattatgaa
    11161 attgcttcat ctgcagtatt ttaaaaaagt attcgaaaca aaaaatgtta cccaagcagc
    11221 caaagaatta tttatttctc aacctgcctt gagccgtgcg atcaaacatt tggaaaacga
    11281 attaggtgtc cctttgtttt accatagcgg cagaaacatt gagacgaccg tttatgcaga
    11341 agaattttat ccatatgcag tcaagacatt agacacatta gaagaaggaa tcaaattatt
    11401 gtcttcggtc aatgagaaga tcgttacttc cgtcatattg tatttagagg tagcctctgt
    11461 ttctattccg aacttggtcc gaatttttat ggaaaagcat cctgatatcc aattaacgat
    11521 catgcaacat catttaccag atgatattac cgatcctgta ctgtacatca cttcggagat
    11581 gaagcctggc atgaccaata ttcctatcat gaaagaacct gtttatgtcg ccattcctaa
    11641 gaaacatcct cttgctaaaa aagaacatct gcaattagaa gatatccaac gcacccccat
    11701 cttgatgctg tcaaagagca acgccttccg tcatacactg gatgcagcac ttgaagcgaa
    11761 aagtatcgaa ttgacgatca gttcgacgac agatgatcct gcgacattac gttctattgt
    11821 caagcaagga ttagctgtta gctttttccc aaaagtttct tggtcttacg ataaaaatga
    11881 tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca cgaacaattt atttatcttc
    11941 tagcttcaca gaagatcatc ctttgatcaa aatgattgca aaaacgatgc gggaatttta
    12001 tttaggaaaa taaaaaaaaa agagctggga caaaattctt gtcgcagctc ttttttcgaa
    12061 caaacggtgt tccagaagta atttcttaga aaataagccg aaatttccca aaaatttgag
    12121 aagcaatttt cgtaaattcc ttcttatttc ttgaagctaa acatttctgt cacaacctct
    12181 tctctattgt cctagacatt cattgcgttg atttaaaact tccatcaatc aaatctgcta
    12241 gttcatctag cttttctatt ttacgtagat caatagagat ccaatgttct ttattcatgt
    12301 gataggctgg aaaatacccc tcttttcctc gcaaaattcc gccaagttca ggatcgacct
    12361 tcaaatcaag aatattgatg atcctatggt cacttgcatc atcatagatt ttttcttctg
    12421 gaacagacat caggacccca aaccattttt tgcttttttg atgtctgaat acgcaataat
    12481 caggaaattt tttaaaaata tattctggag acaaattgta tgtttcattg atgtgatcga
    12541 ttagttcttt tttcgttagc atctgcttcc atctctttct tattgattcg ctttattcaa
    12601 agataccacg taagaataga aaaatgaata caagtagttg ctaatcaaat acttcgatat
    12661 ttttcttttt tatcaactct taaatgttca atccgcctgt gttggtcttg ctaccatc
//
LOCUS       DADVXO010000086        12310 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.089, whole genome shotgun sequence.
ACCESSION   DADVXO010000086 DADVXO010000000
VERSION     DADVXO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.089"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(113..670)
                     /gene="frr"
                     /locus_tag="KB950_001995"
     CDS             complement(113..670)
                     /gene="frr"
                     /locus_tag="KB950_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBB6858209.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(673..1395)
                     /locus_tag="KB950_001996"
     CDS             complement(673..1395)
                     /locus_tag="KB950_001996"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBB6858210.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1531..2412)
                     /locus_tag="KB950_001997"
     CDS             complement(1531..2412)
                     /locus_tag="KB950_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBB6858211.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2511..3293)
                     /gene="rpsB"
                     /locus_tag="KB950_001998"
     CDS             complement(2511..3293)
                     /gene="rpsB"
                     /locus_tag="KB950_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBB6858212.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3652..4131
                     /locus_tag="KB950_001999"
     CDS             3652..4131
                     /locus_tag="KB950_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBB6858213.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4348..5439
                     /gene="phnW"
                     /locus_tag="KB950_002000"
     CDS             4348..5439
                     /gene="phnW"
                     /locus_tag="KB950_002000"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBB6858214.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5432..5560
                     /locus_tag="KB950_002001"
     CDS             5432..5560
                     /locus_tag="KB950_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBB6858215.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5564..6109
                     /locus_tag="KB950_002002"
     CDS             5564..6109
                     /locus_tag="KB950_002002"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBB6858216.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6563..8254)
                     /locus_tag="KB950_002003"
     CDS             complement(6563..8254)
                     /locus_tag="KB950_002003"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBB6858217.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8674..9621)
                     /gene="arcC"
                     /locus_tag="KB950_002004"
     CDS             complement(8674..9621)
                     /gene="arcC"
                     /locus_tag="KB950_002004"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBB6858218.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9736..10755)
                     /gene="argF"
                     /locus_tag="KB950_002005"
     CDS             complement(9736..10755)
                     /gene="argF"
                     /locus_tag="KB950_002005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBB6858219.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10846..12075)
                     /gene="arcA"
                     /locus_tag="KB950_002006"
     CDS             complement(10846..12075)
                     /gene="arcA"
                     /locus_tag="KB950_002006"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBB6858220.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3387 a         2535 c         2207 g         4181 t
ORIGIN      
        1 tttttttatt taaaaactga ataatttttt ctttttttaa tttttaaata aaagaaatat
       61 aaataaaaac agaggaagcg gaatgatccg cccctctgtt tttatgattt tattaaactt
      121 ccaataattc tttttcttta tcagcagcga tcttatctac ttctttgatg ctgttatctg
      181 tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc ttcggtaata tcaccatttt
      241 tttgttgttt tttatattca tcgatcgcat cacgacggat gttacgaatc gcgatttttg
      301 cattttctgc ttctttttta acatctttag ctaattcttt tctgcgttct tctgtcagtt
      361 gcgggataac taagcgaatg acatttccat cattggttgg actgattccg atatcacttg
      421 ccataatagc tttttcaaca tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa
      481 cacgtgcttc tggaatttgg attgatgcca tttggttcaa tggtgttggc gcaccgtagt
      541 agttcacagt aatacggtcc aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa
      601 gttcacgttg aagattttgt gctgcttttt tcattttttc ttttgcttct gtcataattg
      661 catctgccat ttttatttcc cccttacagt tgttccgata ttttcgccta agattgcacg
      721 gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt ggaatatcat tatccatgct
      781 tagtgaactt gcagttgaat ccatgacttg taatcctttg gcaataactt ccagatgagt
      841 cagttcttca aattttactg catttgcatc tactttcgga tcagcagaat aaacaccgtc
      901 tacattgttt ttagccatca ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc
      961 tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc
     1021 tttttctaaa tgacgttctg ctttacggcg aatatatggc tctgcgattt gacgcatttc
     1081 aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc aacgtatcct gtaaagctaa
     1141 ggcattcata actgtagcta acatacccat gtaatcagct tgagcacgtt ccatgcccat
     1201 ttgtgctccg atttgtccgc gccagatatt tccgccgccc acaacgattg ccatttcaac
     1261 gcccagctcg tgaacttctt tgatttcttg gatgatttct ttgattactg gtggtttgat
     1321 tccaaatcct gcgtccccag ctaaagcttc tccactcaat tttaagacta cacgctgata
     1381 tttcggtgtg accatgttca ttcctccaat attttctatt gccattttac catacttaga
     1441 tgatttcgac actctaagac tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa
     1501 ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc acttggctca taacttcttc
     1561 tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt
     1621 aacagttgcg ccttttgatg caacgtattt ttcaacagtc atatcaggat ctttaacgaa
     1681 tggttggtcc accaaagcga tttctgcttt gaatttcttc aagcgtcctt ctaccatttt
     1741 ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt tctgtaagaa cagttttttc
     1801 atgttctaat tcagcttctg gaatttgtga ttcgtttaca taacgaggat tgatagctgc
     1861 tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa
     1921 tacggcaata cgtccgccca tatgcaagta tccaccaaat gcagcattgt cgtctttttc
     1981 tacaacttca aagcgacgga agctgatttt ttctccgata acttgagttg cttcgatcaa
     2041 gtcagattca attgtacctt tatctgtttt gattttcatt gcagcttcca tgtctgctgg
     2101 tttgttttca gcaacaagtt ctgcaatttc ttttactaaa tcttggaaca tttcgttttt
     2161 agaaacaaag tctgtttctg agtttacttc aacgattgct gcagtgttac ctttcacagc
     2221 aactgaagcc aatccttcag ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc
     2281 tttttcacgt aaaagatcaa ctgctttttc catgtcgcct tctacttcaa ctaatgcttt
     2341 tttcgcatcc atcattccta cgccagtcat ttcgcgtagt tcttttacca ttttagctgt
     2401 aacgtctgcc atgtttaact cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa
     2461 ggagagtgag gcttgtcgcc cagcctttga aacagctcca tcattcaaaa ttattctgct
     2521 gaagaattgt cgccttcaac aacatctacg atttcttcga ttgatgttgc tgtgttttct
     2581 tctgtgaaat cttcttcaac tacttgatct tcaccttggt ttccttcgat gaaagcatca
     2641 gccatttttg aagtaatcaa tttaacggca cgaattgcat cgtcgtttga agggattact
     2701 acgtcgattt catctggatc gcagtttgta tcaaccatag ctacgattgg gatatttaat
     2761 ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt caacgatgta cattacatct
     2821 gggattcttg gcatatcagc gataccgcct aagaattttt caagacgttc acgttctttg
     2881 ttcaaaccag caacttcttt tttaggtaga acttcaaaag ttccatcttc ttccattgca
     2941 ttgatttgtt tcaaacggct gatacgtttt tggatagtat cccagtttgt caatgttccg
     3001 cctaaccaac gatggttaac gaagtattgt cctgcacgag ttgcttcttc tttgattgct
     3061 tcttgtgctt gtttttttgt acctacgaat aatgctacgc cgccttcttc agcaacgctc
     3121 ttcatgtaat cataagctgc atctactaat ttaactgttt tttgcaagtc aatgatgtag
     3181 attccgtttc tttctgtgaa gatatatttc ttcatttttg gattccagcg acgagtttgg
     3241 tgaccaaagt gtacgccggc ttctagtaat tgtttcatag aaattactgc catgtttgtt
     3301 tcctccgatt tggttttatt tttcctcttc ttatcttcta ccttgccggc aaactttttc
     3361 aagcacctac cgacaatcca ataagaatgt ggatttgctg atgcataaag caccctcagc
     3421 tattatacag acaaaagatc cgtatcgcaa gagcaaatat acttttctgc tcaacattca
     3481 atagtttacc tgaaagataa aaagaattca agaaaaacag tagatttctc tttgtttctt
     3541 tattatcctt tttccataaa ataattttag gtataaaaga cgaatattat tgggcaatcc
     3601 ttcttttttt tgttatgata catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa
     3661 gctcaaagac atttgctgat taaacaagtc attgaagaat tcccgatccg tacgcaagaa
     3721 gaactattaa acaaacttgg gcattatggc gttaccgcca cgcaggcaac gatttctcga
     3781 gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta
     3841 ttgtttcgcg aaaaaggaac tcctgagact aggactgagg aagaggatcg gctggttcaa
     3901 atgattgaag acattgtatt gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc
     3961 ttgccagaca atgctcattt atttgcatct gttttagatg agatcaaacc tccctctatt
     4021 gtcagtacaa ttgctggatt cgatacgatc atggtcatct cggaaaatga ggaggacgca
     4081 aaacgagtag aagaatttct tcaccatcca actgaagtat cactttttta atcagagcac
     4141 gtattcctaa tatggaatgc gtgttttttt attttcaata ttttccttat tgaataaacc
     4201 tttacactct ttacaaaaat acatcatttt ttattcaaat ttttcaacac cttgattgaa
     4261 tcacaataat catcatttat attggatgtt aacaagagca gcttcttgct ctttgtgtat
     4321 ttcaaaaaag aaatgaaagg aaatcatatg tataaattat taacttctgg accattgacg
     4381 acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt gtacttggga tcaagaatac
     4441 aaagaaatta cacaatggat aagaaaaaaa ttattggatc ttgctcaagt atcggaagga
     4501 atatacacaa cggtcttaat gcaaggtagc agctccttcg gggtagaatc ggtattaagt
     4561 tctgtgatca ggccagaaga tactgtactg atttgttcaa acggcgcata tagacaacga
     4621 atgatcaaca tgtgcgaatg tctgaaactg aactataccg tttatgaggt atcggaacat
     4681 caaatcctcc aagctcatgt gattacacag ctgattgaag agaatccgtc tatctccgca
     4741 ctagcaatgg tccatagcga gactacttcc ggtattttga ataatttgga agagattgct
     4801 gctgtcacac aagaaagaga tcttttgttt atagtggacg cgatgtcaag ctttggtggg
     4861 ataccgattt gtgtagccga actagggatt gattttttga tttccagctc aaacaaatgt
     4921 attcaaggtg ttccaggctt ttcttttgta cttgctaata aagcggaact ggaaaaacga
     4981 aaaaatgtta gccgaagttt gtcacttgat ctatatgacc agtaccgcgt gatggaaaac
     5041 gagaatggca aatggcgttt cacttcccct acccatacgg ttttggcttt tcaacaagcg
     5101 ctgaaggaac tagaggcaga aggaggaatc gaaactcgat attcacgtta tctcgccaat
     5161 aatcatgtgc tacgacaaaa aatgacagag atggggttcc agcctttcat tcaagaatat
     5221 caggggccat tcatcacctc ttttctttac cctaaggact ggcgatttga ttttaatcat
     5281 ttttatcagt atttaaagca aaatggattt gtgatctatc ccgggaaaat cagcgatcat
     5341 gaatgttttc gtatcggaaa tattggcgag atctatccgg aagatatttt ggaactgaca
     5401 gcaattatca aagactataa ggagaacgtt aatgattaaa actgtaattt ttgattgggc
     5461 aggaacaact gtggactttg gctgtatggc tcctgtccac gcttttagaa atgcttttct
     5521 agaaaaagga atccaactga ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg
     5581 ggatcatatc caacagcttc tttccctccc ttctgtcaaa aaacaatgga tagctattta
     5641 tggacaacta cctcaggaaa aagaggtgga ggaattgtat caacaatttg aacattatct
     5701 gtttaaagat ttggtcaagc attctacctt gaaacccgac acattggaaa cgatcgaacg
     5761 gttgagaaac gctgggatca atattggttc cacgaatgga tttaatgctc ctatgatgac
     5821 aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca gacttcatgg caacttttga
     5881 agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc cataaaaaca tgcagtactt
     5941 caaaaacaag tcggttgatg aagtaatcaa agtcggagat acggtctcag atatccagga
     6001 agggaaaaat gccggagtgt tgacagtagg tgtaattgaa ggtagttcgc taatggggat
     6061 ttcttatgat gagtaccaaa aattagagat ggggaaaaag atcgtttaaa acagtctgtt
     6121 gctcttagat tccaacaagc cggcgctgat atttgtgtaa acaatttaaa agagttaaca
     6181 gaatacattt tgtacgctga catgaatcca gaatgacttt tatacaaaat aagaggttgt
     6241 gaaaaagctg tcctgtatca agtaataaaa acagccaagc aaaaatagct cctcatattt
     6301 ttcgtttggt taggcttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
     6361 aaaaagggag ctgtgacacg acttttgtca cagtcccctt atttgcaaac aaacagtgtt
     6421 taagagacaa cctctccact aaacggtgtt cattcagctg actctcggta tttgctcaaa
     6481 aaacgataaa atttgaaaag ctattttctc ttttgtttct ctaatactcg gatcagaacg
     6541 ctgatttcac attctctttt tcttacatct tatctggtgc atgaagtcct agcaaacgaa
     6601 ggtcttcttt caataataca gtcactgcat aaactagagc taagcgtgct tctttttgtt
     6661 catcatcagc taaaatcttc gtatgagcat agtatttgtt gaatgcttgt gctaatttga
     6721 ttgcatgttt cgcaatcact gacggttcgt atttttcccc agcagacaag accgtttcag
     6781 ggtatttttg aactaattta accacttccc agctggtatc gtcattcaat gcatagtctg
     6841 ctgtttctga agggacaaat cctgcttttt ccaacaggct gactgcacga gcatgtgtat
     6901 attgcacata aggtccagtt tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa
     6961 atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt
     7021 tagctacagc atctttgttt tccaaatcag ggtttttctc gctgatttgt tctttagcag
     7081 attggattgc ttcatttaat acttcttcta ataagacgat ctttccttta cgagttgata
     7141 attttttacc accttgcgtg atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt
     7201 caaagcccat ttccttcaag acagctttca actgtttaaa atgatagctt tgttcattcc
     7261 ctactacata tagagattgt ttgaagtcgt aagtacgttt acggtacaat gcagcagcta
     7321 agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt
     7381 cataagcaga taggtcaacg atctctgcac ctttatcctc gttcagcaaa tgtttttctt
     7441 ctaaaagttt gacgatttca tccatcttgt cattgtagaa agcttcaccg ttcaacgaat
     7501 cgaaacgtac ttccagcaga tcatagattt tattgaattc tttcatcgat tcatcacgga
     7561 accattgcca taattgaata gcctctttat cgccttcttc cagacgtttg aaccaagcac
     7621 gagcttcttc atttaattct ggttctgttt cagctacttc atggaattgg acgtataaac
     7681 gaagcaattc attgattggt tctgctttaa cagcttcttc tgtaccccat tttttgtaag
     7741 cgacgatcag ttttccaaac tgtgttcccc agtcacctaa atggttgatg cggattggct
     7801 gataaccgat tttttccata atgaaaccga tcgaattacc gataactgtc gaacgcaaat
     7861 gtcccattga gattggtttg gcaatattag gagaagacat gtcgattggt actgttcctt
     7921 gattcccgat gttgctatcc ccataatgtt ctttttcttt cactactgtt tgcaaaactt
     7981 ttttgctgat aagttcttta ttcatgaaaa agttcaagta aggtccgaca acttcgattt
     8041 tttcaaagtt ggcagaatcg attttttctg ctaaatctgc agcgatttgc tgtggcgctt
     8101 ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt
     8161 ttggattttc tagtaattgc tcgacttgtt ccagtgttaa atcatctttg acagcgtcat
     8221 agacagcttt tgcgacaaga tctttattat tcatctttat tcctcctaaa gtgttccctt
     8281 gattgttaaa caaaaaaaat ctctagcgac ggcatactta gtatgccatc actagagacg
     8341 agatttcccg cggtaccact ccaattgtcc taaaaaagga ccagctccat ctgatatcgg
     8401 tagacgaccg tctgtttaaa acagaattct ccaagtgcgc ttcgttgtat cttcttcatt
     8461 cggcttccac cacttccgaa ttcgctagcc aagaatgata caagtactct cttgttcaac
     8521 aaatcttagt cttattaaac tttcttgaat tatatcagaa aaaaaaacga aaggctagtc
     8581 gtttcataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
     8641 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
     8701 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
     8761 ttttgcttca acaaatgcaa tagctgcttc tactttcgga agcatgcttc ctggagcgaa
     8821 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
     8881 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
     8941 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
     9001 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
     9061 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
     9121 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
     9181 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
     9241 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
     9301 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcgttttgta accagtaacc
     9361 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
     9421 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
     9481 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
     9541 ttcttgttgg gcttttgcac ttgcatccgt agacaagatg gcattgcccc ctaaagcgac
     9601 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
     9661 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
     9721 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
     9781 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
     9841 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
     9901 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
     9961 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    10021 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    10081 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    10141 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    10201 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    10261 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    10321 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    10381 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    10441 tccgtcaaac atacgtccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    10501 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    10561 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    10621 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    10681 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    10741 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    10801 aaatcccgac ggcagctgac agtaagactg ccgtcaggaa acaaattact tcaagtcttc
    10861 acggacaagt ggttggctca tgcagcgtgg accaccacga cctcgtgaca attcacttga
    10921 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    10981 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    11041 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    11101 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    11161 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    11221 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    11281 gttgttcgga attttgattg ccaacacttt ttcaaatcct gaattttttg cgaataggtg
    11341 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    11401 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    11461 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    11521 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    11581 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    11641 gtcatcgtct gctgacagat catatagact gctagaacgt acttcgatat cttttgtacg
    11701 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    11761 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    11821 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    11881 ctcgacgcct tcattttgca atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg
    11941 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    12001 ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    12061 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    12121 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    12181 ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt cattttttat
    12241 tctgtataca ctttcaaaaa aatatttatt cattgaaaaa atttttttga ataaattaaa
    12301 aaaataaatt
//
LOCUS       DADVXO010000087        11994 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.090, whole genome shotgun sequence.
ACCESSION   DADVXO010000087 DADVXO010000000
VERSION     DADVXO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.090"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(2..331)
                     /locus_tag="KB950_002007"
     CDS             complement(2..331)
                     /locus_tag="KB950_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBB6858221.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            508..1236
                     /gene="sfsA"
                     /locus_tag="KB950_002008"
     CDS             508..1236
                     /gene="sfsA"
                     /locus_tag="KB950_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBB6858222.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1252..2265
                     /locus_tag="KB950_002009"
     CDS             1252..2265
                     /locus_tag="KB950_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6858223.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2265..3542
                     /locus_tag="KB950_002010"
     CDS             2265..3542
                     /locus_tag="KB950_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6858224.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3605..6307
                     /locus_tag="KB950_002011"
     CDS             3605..6307
                     /locus_tag="KB950_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6858225.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6459..6776
                     /locus_tag="KB950_002012"
     CDS             6459..6776
                     /locus_tag="KB950_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858226.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6806..7126
                     /locus_tag="KB950_002013"
     CDS             6806..7126
                     /locus_tag="KB950_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6858227.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7234..8694
                     /locus_tag="KB950_002014"
     CDS             7234..8694
                     /locus_tag="KB950_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858228.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8762..8983
                     /locus_tag="KB950_002015"
     CDS             8762..8983
                     /locus_tag="KB950_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858229.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9014..9196
                     /locus_tag="KB950_002016"
     CDS             9014..9196
                     /locus_tag="KB950_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBB6858230.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9196..9609
                     /locus_tag="KB950_002017"
     CDS             9196..9609
                     /locus_tag="KB950_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBB6858231.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9732..10913
                     /locus_tag="KB950_002018"
     CDS             9732..10913
                     /locus_tag="KB950_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6858232.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10984..11148)
                     /locus_tag="KB950_002019"
     CDS             complement(10984..11148)
                     /locus_tag="KB950_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858233.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11302..11375
                     /locus_tag="KB950_002020"
     tRNA            11302..11375
                     /locus_tag="KB950_002020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11336..11338,aa:Arg,seq:cct)
     gene            complement(11444..11719)
                     /locus_tag="KB950_002021"
     CDS             complement(11444..11719)
                     /locus_tag="KB950_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6858234.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         3946 a         2020 c         2513 g         3515 t
ORIGIN      
        1 tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt
       61 cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa cgatattcat
      121 ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg taatcagatg
      181 taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct tgatactttg
      241 agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa cttgagagaa
      301 cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca ctcctttatg
      361 agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta
      421 aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat
      481 acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt
      541 attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt
      601 gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct
      661 attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag
      721 aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt
      781 ttagacggca cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa
      841 gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca
      901 tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg
      961 cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat
     1021 gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa
     1081 gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc
     1141 ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt
     1201 gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac
     1261 ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca
     1321 aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt
     1381 tctcattatg aaggggatat cgaattcgca acagatttga agactttttt caatattact
     1441 catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa
     1501 gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa
     1561 atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga
     1621 aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata
     1681 atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga
     1741 gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt
     1801 taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca
     1861 agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt
     1921 gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt
     1981 gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat
     2041 cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat
     2101 gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg
     2161 tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa
     2221 aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac
     2281 aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc
     2341 ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc
     2401 ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag
     2461 gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag
     2521 atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag
     2581 ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac
     2641 caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa
     2701 tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa
     2761 ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt
     2821 cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag
     2881 aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag
     2941 atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc
     3001 aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa
     3061 gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga
     3121 aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat attacaggtc
     3181 ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg
     3241 gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc
     3301 taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat
     3361 acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata
     3421 aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa
     3481 taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat
     3541 aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc agaggagggg
     3601 ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc aacaaggatt
     3661 gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa gccgatattt
     3721 aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag tcgtttactc
     3781 tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta cgcaagtgac
     3841 ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc aggcaaaagc
     3901 tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa ctggtacagg
     3961 gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga tgctttctgc
     4021 agatgcgcca tttgtttctt tcaattgtgc ggattatgct caaaatccgc aactgctctt
     4081 tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag atagtcccgg
     4141 tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc gtttaccgcc
     4201 ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc cgcttggaga
     4261 aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg aatcttcaga
     4321 aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc cgaatcttgc
     4381 ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac aagaagcgaa
     4441 tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca tgctttatga
     4501 tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt
     4561 tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag aactttccct
     4621 tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg accgattcat
     4681 ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg tctggtcaca
     4741 agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca cttctctttc
     4801 cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg tggatgctta
     4861 tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc ttttgccaga
     4921 tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt tagaccgtat
     4981 ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat tggatcgtgt
     5041 gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa attttaagag
     5101 ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac aagttgagat
     5161 tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag gagaaagtga
     5221 gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac agactgcttc
     5281 aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag cttttaatat
     5341 gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta
     5401 tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat taaacacctt
     5461 cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga tgacaagtac
     5521 catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag aagatattta
     5581 tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc
     5641 tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg aaggagtagc
     5701 tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag aactgatcca
     5761 gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat tggaagaata
     5821 tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc catttttttc
     5881 agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag ctgatgatga
     5941 gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg tcttttctat
     6001 tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg atttgtcgat
     6061 tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca tgttagacgg
     6121 attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct caaaaattta
     6181 tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt
     6241 aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa ataaaataaa
     6301 aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc actataaaaa
     6361 agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat gcttcttgcg
     6421 ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt
     6481 tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa aaagcagcag
     6541 aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca gataataatt
     6601 tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc atgaaagctc
     6661 aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat atgcaagact
     6721 atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg aaataaatca
     6781 agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag ccatcatggg
     6841 gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg cggctgctaa
     6901 aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat cacttataca
     6961 agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc atatgcaagt
     7021 gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt ttactgattt
     7081 agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac aacttctttt
     7141 ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag gagaactctc
     7201 tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag ttggatggag
     7261 aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt
     7321 gatgctttta tcggcatact gcccgcaaca atggcgggat cagtcgctgt aatgatcaat
     7381 gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag tttagcagca
     7441 gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt tgtctggaac
     7501 ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa tcttgcccga
     7561 gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt gattcaaggt
     7621 atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc aaatgtcgca
     7681 gatgcaatca atagttctga gattggtgca acgattgcag atggcacact gtcagttgga
     7741 ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac agttatgatc
     7801 atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat tacgatcaaa
     7861 atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt accagcgacg
     7921 atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa
     7981 ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt atctcaaggg
     8041 ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt tggtattcat
     8101 ggatcaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca actaatcaat
     8161 attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt gttagatgca
     8221 atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt
     8281 ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc taaacgtgca
     8341 gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat caacgaacca
     8401 gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt cttgattgct
     8461 ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt cgctccggta
     8521 tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc aacaggagcg
     8581 gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt gatttgggca
     8641 ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca ataagatgaa
     8701 cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag gaggaactga
     8761 aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt tagaaggata
     8821 caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc tgttctttga
     8881 aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa tcaaagcaca
     8941 gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg tttaaaaaga
     9001 taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt ctttgatcgt
     9061 gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg tcacaggtct
     9121 ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag aagaagcaaa
     9181 ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc gtttttttac
     9241 gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa atcagtaact
     9301 agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc
     9361 ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc gacaacgaag
     9421 aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg tgaggttcat
     9481 tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat gcttttagga
     9541 acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat gatcgaagca
     9601 acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag taagctagaa
     9661 ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt tttgtaggaa
     9721 ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt gaggaaatgt
     9781 ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga gaacgttttc
     9841 gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat cggctaacaa
     9901 gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac ctaatggccg
     9961 gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc aatcagatca
    10021 tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat ctggcgccat
    10081 tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa atcagttatc
    10141 aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg
    10201 attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg gaaaaccaac
    10261 gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa aaaggcaatt
    10321 accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc gtttatcagc
    10381 ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat gctttttgca
    10441 gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa tattcatgcg
    10501 gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct gtaatgatca
    10561 caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat tatccattta
    10621 aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg aaatggaatg
    10681 aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa
    10741 caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta ttagggtatc
    10801 gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa
    10861 cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt tgataaaaaa
    10921 cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat ttgaaggaaa
    10981 atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg
    11041 gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat tttttttaga
    11101 tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg gaaaggacct
    11161 ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt ggaaacgttt
    11221 tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa acaaacaagc
    11281 tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat cgcctcctaa
    11341 gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg tatcctttgc
    11401 ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta tcatttttat
    11461 ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
    11521 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
    11581 taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt agtccatcaa
    11641 taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt actaggtcga
    11701 cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga ttggcttata
    11761 ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac ttgaagcttt
    11821 acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt aatagctgat
    11881 gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaacggt ggggttgttt
    11941 agcgaaataa aggatctaag ccaatagctt agatccttta ttctatgctt tata
//
LOCUS       DADVXO010000088        11816 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.091, whole genome shotgun sequence.
ACCESSION   DADVXO010000088 DADVXO010000000
VERSION     DADVXO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.091"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(5..211)
                     /locus_tag="KB950_002022"
     CDS             complement(5..211)
                     /locus_tag="KB950_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858235.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(227..841)
                     /locus_tag="KB950_002023"
     CDS             complement(227..841)
                     /locus_tag="KB950_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBB6858236.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(988..1671)
                     /locus_tag="KB950_002024"
     CDS             complement(988..1671)
                     /locus_tag="KB950_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBB6858237.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1780..2238)
                     /locus_tag="KB950_002025"
     CDS             complement(1780..2238)
                     /locus_tag="KB950_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBB6858238.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2322..4085)
                     /locus_tag="KB950_002026"
     CDS             complement(2322..4085)
                     /locus_tag="KB950_002026"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBB6858239.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4306..5766)
                     /locus_tag="KB950_002027"
     CDS             complement(4306..5766)
                     /locus_tag="KB950_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858240.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5974..6900)
                     /gene="glsA"
                     /locus_tag="KB950_002028"
     CDS             complement(5974..6900)
                     /gene="glsA"
                     /locus_tag="KB950_002028"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBB6858241.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6923..8350)
                     /locus_tag="KB950_002029"
     CDS             complement(6923..8350)
                     /locus_tag="KB950_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6858242.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8381..9595)
                     /locus_tag="KB950_002030"
     CDS             complement(8381..9595)
                     /locus_tag="KB950_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBB6858243.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9936..10178)
                     /locus_tag="KB950_002031"
     CDS             complement(9936..10178)
                     /locus_tag="KB950_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6858244.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10210..10767)
                     /locus_tag="KB950_002032"
     CDS             complement(10210..10767)
                     /locus_tag="KB950_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6858245.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10780..10968)
                     /locus_tag="KB950_002033"
     CDS             complement(10780..10968)
                     /locus_tag="KB950_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBB6858246.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10981..11547)
                     /gene="amaP"
                     /locus_tag="KB950_002034"
     CDS             complement(10981..11547)
                     /gene="amaP"
                     /locus_tag="KB950_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBB6858247.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3466 a         2447 c         1934 g         3969 t
ORIGIN      
        1 atatttattt gttaaagtaa ttaaacaatc cgctaatcgt ttgatctggc atttctattt
       61 ccttaccatc taaagtaaca gtagtaacga caaaatggtc ttgcaatggc ggaacttcta
      121 agtactgccc ttgcagaaaa tcattcactg tttcatattc ttttttcatt tcttttgaat
      181 ctgtcgatgg tttatagctt atttttaaca taactagtgc tcctttttat tcgggacgtg
      241 caatcgtgaa aatttctcct attgctgcat aatcatttcc tgccaaacgg cttacagcat
      301 tcaattttct tggatcgatc cttcctgtct tatcatcata gactgtttca tttaaatgat
      361 atttctgtac ttctagcaaa aataaatcag ctgttacttt atttttattc cgtatctcga
      421 catgttcata taaggatgtt tcaaaacgaa cttttgcttc tgtcaatgct ggtactgaaa
      481 tgattactga atcagttaaa gtcaagtcag tcaggtacag ttcgctttcc tcattaggca
      541 gattggctgc tgttttattt acttcttcta cgttgtcctc atcaaggata tggataacag
      601 cttctcttgt ctcaagaata ttctttgctg tgtcttttac tgcatcttgt tttctctgga
      661 tcgccaagga taagatgggt cgattcgaac tgacgatatt gaagtaactg aagggtgcga
      721 tattcactat atcttttgct gatttagtcg ttactactgc tactggtcta gggatgactg
      781 aaccaatcag caacttgtaa ttttcacgtt ctgtcaattc tgaaggactc cgttcaaaca
      841 tggtttcgcc ttctttctat aaaattcgtt aaaaagcaga tcttgaagac aaaatgaaaa
      901 gtaatgcttt tttcatcttc ttagaatctg ctttaaacta tctatcaatt tttctttttt
      961 gtcaagaatc tgtgccgtgc gtgtttttta gttatttgct ttacttgcgg cttcgacata
     1021 tcttttagtg atcaattgcg gacgggtgat aatagatcca actacgactg aaaaagcacc
     1081 taattcatat gctttctcta cttgttcagg agagtcagta tgtccttcca taattactgg
     1141 gatatcgact tgttctatga tttgattcaa taaatcaaaa tcaggtgtct gcgtctttcg
     1201 tgaatattct gtatagcctg ctaaagtcgt agacaaaaga tcaaaaccat cttcttgcgc
     1261 ttgaagcgct tcctcaaaag tcgaaatatc tgccatagct aaccgaccgt gttcgtgaat
     1321 catttggaca agttcagcag ttgttttttc tgtcggacgt ttacgagaag ttgcatccag
     1381 tgcaatgatt tctgcttgcg tctccaaaag ttcttccact tctttttctg tcgcagtaat
     1441 aaacacttct gacccatcgt aatctctttt gataatccct attaccggta aatcaacgat
     1501 tcgcttgatt tcattgatat cttctgtcgt atttgctcga ataccaactg ctccgccttc
     1561 ttttgcagcc aaagccattc tccccataat aaaagaactg tgtagcggct cgttttccaa
     1621 agcttgacaa gaaacgacga ctccgccttt gatttgttcc agtgtatcca caatatctcc
     1681 tccagtaatg ttatataaaa atacagcttc atataaaaat gtaacccttt tctattcagg
     1741 agacaataat gagctatttc tttcagcaga cctttcttac taaaagaaaa ttacttcaaa
     1801 ttcttcgtat acgaccagct tctcttctgt aaattctatg ttcaacgctt ctttataagc
     1861 atgtttaaaa aactcagcat ggactttccg ccaataagct aatgtccgat ccccttctcc
     1921 ttctttccaa gcatgctctt tcgtgacttc actaaaaggc aagatggtca catttatatt
     1981 tttgatgata caaactggtt cattttttcc attcaggacc acgtctaatt gaccaacttt
     2041 tggtaaaggc tcattctctt gtttatacag atcgtaacca cttgatgtac cagtctttat
     2101 cttttttaca actagttcgc tcaactcgtc tgccattttt tctgtatccc caaatgaaaa
     2161 gaaacctaat ttttctgatt gataactagg atatttttct tggaactttt tgacatattc
     2221 ttctacttgc tgatacataa catccacccc tttgcttttt ataatgaaaa aaccatccag
     2281 atactagtat aaacaattat ctggatggtg agcataatga attattgaat attcaagaca
     2341 cgcagactat cttcaacgat ttgttttggt gttaaatgat aacgttcata aagctgccct
     2401 acaggaacgc tatccgtgaa ttctttctct gcaccaaaat ttaatacgtt cattgaagaa
     2461 tcaccataat aacgactgat attttcgcca aatcctcctg acaaactacc atcttctaat
     2521 gtgatgacta attcgtgtgt ttcttttaat ttatctaatg tttctacgtc attttctgtg
     2581 atcgttcctg gattgatcaa cgttgcttcg attccttttt ttctaaaagc ttttactgtt
     2641 tcttttccta attcaagaaa accgcctaaa gctaaaattg ctacttttcc tccattattt
     2701 actgtttgat aggctggaat actataatca tttgcgatag ctgtaccatg ttctacacca
     2761 tgtgtaggaa ttcggattgc tacaggctgc gctgtttgtt cgaacgccca atctagcata
     2821 gagatcatat cctcttttgt tgctggagca agatatttga tgttcgggat acttgataag
     2881 taaggaatgt caaatgttcc ttggtgtgtt cggtcactag ctgagatcgt tccgccaccg
     2941 actaaaataa tcactggatt ctcgttgatc gctaaatcat gtgataattg atcatatgcc
     3001 cgttgcaaga aagtacttga ttggaagacg actggacgta ctccctgaga agccaaagca
     3061 gaagcgaaag tgatagagtg ctgttcagcg atccccacgt caaaataatg atctggatgt
     3121 ttttcttgga atgttttcag tccaaaggcg cctggtatcg ctgcattgac tgcggcaata
     3181 ggagctgtac ttgtttgcat tttcttatcc aaataatcca tgatgatcat attaaatgtt
     3241 tctccagaag ttacttgctt tgcctccccg gtggacagat caaatggtac atgccaatga
     3301 tatttcattt tattatcgat tgccggctga taaccgcgac ctttttccgt atggacgtga
     3361 agtacaatcg gatgatcaat atcttttact tcttcaaata aacggatcac cgtttccaga
     3421 tcgtttccat tatccacgta acgataatca aatcccattg ctttaaatag gttattgctg
     3481 cttttaccgt tggtttgacg aagctcagct aagtttttat ataaactgcc atgattctca
     3541 tcaatagaca tctcattatc gtttactaat acgattagat tggattgtaa aacaccacca
     3601 ttgttcaatc cttcaaaagc taagccacca cttagggagc cgtcaccgat aaatgctacc
     3661 acatttcctg attctttctt cagatctctc gctttagcta aacctgttgc taagctgata
     3721 gatgtagatg tgtgccccac tgtaaaatag tcatgtttac tttcatgttg gttggaatat
     3781 ccgctcactt cacggtaatg tcctttttca aaaccatttt ttcgaccagt taaaagctta
     3841 tgaccataag actgatggga aacatcccaa acgattttat cctttggtga atcaaataca
     3901 taatgaaacg caatcgttaa ttctactaca cctagattcg gaccaacgtg cccacctaca
     3961 tcgctgtcct tttgcaagat tttttggcgc atctcttctg ccaactgttt catttcctca
     4021 acagataatt ttttgacatc tgcggggcta ttgattgcat ccaatacatg taatactgta
     4081 ctcataatac attctctcct ccgtactatc ttcttttaat gaaaatagat ttgttacact
     4141 ttatctattc tactacttag agttgactct aagtcaatag cattttaaaa aaatttcaga
     4201 gaaagcttgt tatataagta gatcttcgca cgaatcagga ctcaaataaa aaccttctac
     4261 agacaattaa aaagctttgt agaaggtctc ctaattaagg tctcatcaag aaatatgttt
     4321 ttttaatagg tgaagttcct gagcaatatg atacaaatgg atgtttgcac cttttaatga
     4381 gattggatag atctttgtct tttcagtttc taatccattt tgatagctag cagcatcgca
     4441 caaaaccact ctttttttcg cacatagaca ttcatcaggc aattcttcta atgaacgaaa
     4501 taaaatagga tttatttcag cgatgccttc ttccgcatac aaatgaaatt gggttcgata
     4561 aaaaaattgt ttgactgaac tttctgaaac aacaaaaatt tctatttcct gtctccccgc
     4621 ttgttcactg ttgatcaaag aaagactttt gacgaatttt cgaatgaaat tttcattcca
     4681 tcttagttgg atttgatttt tctttttcca ctcttccaat atttctacaa tttttccgta
     4741 cagaagttgt tctttttctg atagaagata gatcctttcc ggtaaaaata cttgtccatc
     4801 tgcccacaac gtacggacaa attgtaaaaa ggattgcttg aaaataaaat tttctgcatc
     4861 gtcgatttca gcgatactaa taatctgtaa aatcaattca ttcagtgata ggtcattgag
     4921 aacaaaattt ttataaacaa cttcgaaatc ttttcttaac agctccatgt tctcattggt
     4981 ataatttcta ctgttgaaca aagaaaaaat ataatagaca tcttcttctt gtaagtctag
     5041 aattcctttt tgctcttcca atccttgaca gactaattga aataacggct tcgtttctgc
     5101 gcttttctta tcttcttctt tcagtccaag gggaaatgcc tttcttccga ttgcgatctc
     5161 tattcctcgt atgataaagg acttttcatc ttccgaatat cttcttttaa aaaaatgagt
     5221 ctctacataa tcaatcaact cgttacaagc ttcttctaca tacggatttt tattttcata
     5281 gccttcccaa ttgacataac gtactaaagc tagcaagatg ttccgcgagt caaattctgg
     5341 gataatgatt tctttatttt tcaaataatc attttcttta aaaaaatcca atacgatttt
     5401 tttgattgta tatactttgc tcaaagaaat aaattcaaga tcagccagtt ctgtactcgt
     5461 gaatatacca ttaaaataat aacttaaaaa ctttaaaaaa taagattgac cgtacagaaa
     5521 ctgtgtgagc tcagaaagag caataccatg tttgaatacg agtttatact tatgcttctc
     5581 tcttacttgt tcttttatac aatattctag acactcggct agactttcta gatcgtgaag
     5641 gatcgttggt gtagtcaccc ttagagaaat agctgtttgt tcgatgtcta tttcttggaa
     5701 ttcaaataat tgttcacaca gatacacttt tctaaatata tttcgctcga tgtaattttc
     5761 cagcattcct gatacacgcc tccagttttt cttatgattt tatttaatca tatctgtaaa
     5821 tcgtattttt gtttcctttt tatttagaaa agccctttca aaaaccaata aaaaaagaaa
     5881 ctgggacaaa agtcaaacaa gctcacaatt tcagcctcat tgtaggtagc tttgagccgt
     5941 ttggactttt accccagcct ctttcaagaa tggttattca aagatgtcag cgtctagttc
     6001 tttgactaca tctttcaaca attggatacc agctgcgctg ttaccaaatg gatctagtgc
     6061 cgggctgaag acacctatgc cgtaacgatt tggcacagct gccatcaacc cgccgcctac
     6121 accgcttttt gcaggtacac caacatggac ggaaaactct cctgattcat catacaaacc
     6181 agcagttgtc ataattgatt tgacgattgt agcactttct tcagtaatga tttgtttgcc
     6241 attccaagga gcaattcctt tatttgccag cacagcagct aattttgcta tgcctgtagc
     6301 ggtgacattg agggagcatt gtctgaaata agcatcgagt atctccggta cgtctccttc
     6361 gatcatttga ttacctttca tatagtaagc aagagagcga ttgatatcac ctgttcttga
     6421 ttcagaatga tagatttctt cattcaatgt gatattagga tcatcacaga tctctttcat
     6481 aaatgacaag attcgctcaa atttttcatc tgcatcttta ccggcaatca acgaggttgt
     6541 tgcaatcgct cctgcattaa caaaaggatt cattggatgt ttgcgatgat tgatttccat
     6601 attcatgata gaattgaagg caaaaccggt tggttccgtg ttgattgttt gaaagacttt
     6661 ttctattccg ttgtctttga ttgctaataa caatacggga acttttgaca tgctttcgat
     6721 ggcaaagcgc acatcggcgt ctcctccctc aatcagttgt tttgtatcca aatcataaat
     6781 cgccatacct aattgtttgg gatccacttc agctaatgct ggaatgtaat ccgccacttg
     6841 tcctgtttga taatagtgga aattttttcc aatcaattgt tctaattctt ttacttccat
     6901 ttgaataacc agctttcatt ttttattttt ctgcatgctg gaatttatga cggcatgcgt
     6961 agatcgtaaa tggaataact aagacgataa gataagaaac aatcagaatc gtttcataaa
     7021 tcccatgctg tccagctgcg atactagcag gtggaacaaa tgagatacaa aaagcaaaaa
     7081 gtgaagtcaa aagacctaaa caagcaacga ttacttttac aacttttccg cctggaacct
     7141 gataggctct ttcaagactg tcatctttaa aaaccagctt caaatagccc gcgaaaaaca
     7201 taaagtacaa catcaggtag attacaacag tcaatgacat cgctgcaaga aatgaaagat
     7261 tattcccccc gccgcttaat gtaaagacgg ctgcccagat tgttgcaacg atcccatttg
     7321 cgatcatcaa atgagcagga acaccgtgtg tgttttcttt tgcaaaatat tcaggaagta
     7381 atccttcttc tgctgctacg tgaagtgctt ttgaaggaga ggttacccaa gaagcaattt
     7441 ctccgatgac acctaaacaa acaagaatcg caagaatttt cgcaatccat gcaagatttg
     7501 gatcaatgtg tcttaatagg aattctaacg attgaatcac ccctgtatta agagacaaac
     7561 catgttgagg tactgtagta gcgacggtca atccgccaaa agtatctaaa atgattgcca
     7621 gaataaccaa tacaaacatt gctaatggat aatccttttt agggttttcc atttcatttg
     7681 catgactggc agatgtttct acgcccatat atgctaaaat aaaggaaaca aaaacaacca
     7741 gcgtaggcaa tttcgaaaaa tcaggaatca atgttttcat agacagtatc gtttgtagtg
     7801 gagcgcctga agcgacataa tggatcccta agccaaacaa gactaatgct ggcaacaaga
     7861 taccagctac aaaaccgatc cgagcaatca atgaagtgta tttggttcct tttaactgtg
     7921 agagtaaaat agcccagaaa ataatcaata ggattgccaa tttcagatat ttattgtttt
     7981 gtatagcacc aatacccgtt gcataggata atgcaccagt taagaaatac agcatggtca
     8041 agtagccaac tgttatctca aaccattgga aaaagattgc ggcaaaaccg aatcgttttc
     8101 ccaatgtgcg actgacccaa gtatagacac cgcctttttc ccatcctttc accgttgcca
     8161 tctctgctgc gcacaatgcc acagggataa accatagcaa accgcctaat aacaagaaaa
     8221 atactaagct gaagcctgag gtcgcgaatg tgggatattc atataatgaa acgaccattg
     8281 agatggtcat agccaaaaag ccaaagaacg ttaatttttt tgcaggaatt ttcgttttgc
     8341 tttgttgcat gaatcttcct cttttctatt tgaatttgtt tcaattgagt ttgttgccaa
     8401 agaagaaaaa gtccaatcct tcttcttgtt cttcttttgt agtagtgatc gttgtaaagt
     8461 gcatcgccga ttttaaaatc actgttgtca gcacataggc atacacggca gtaatgatga
     8521 caccaatcac ttgaatccct aagaagtacc agctgcttgg attcgttagt aaatggctgt
     8581 tagatgcaaa gatccctatc agaatcgtac ctgtgaatcc gcccatgcca tgaacacccc
     8641 acacatcaag tgcatcatcc cattgtttct ttttacgaaa atcaacagca gcatgacaga
     8701 cgatacccgc aatgatccca atcagcatgg cactttttgc ttctacataa ccggcacaag
     8761 gcgtaatggc tgccaatcca gcaacggatc ctgttagtac gtcgacaaaa tcaaagccat
     8821 ttccttttac ttttgcaatg atcagccaag taatcatcgc aaatgcaaga gcgatgaatg
     8881 ttgaaccaaa tgcattgact gcttgttcat ctgctcgaag tgcaccaccg gaattaaaac
     8941 cgaaccagcc aaaccaaagc agtcctgtac cgatagcagc tgccataaga ctgctatgct
     9001 gacttctttc tcctaaagga atctttcttt gaccaagtgt gaggatacat gctaaagagc
     9061 cgaatccagc tgtcgtgtgg atgactgctc caccagcgaa atctctaaat cccatctgat
     9121 ccaaaaatcc gccaccccat atccagtggc aaacagggaa ataaatcagc agattccaaa
     9181 aaataacaag tataatatag ccttttaaat tcaatctccc tgcaaaagct cctgtcatca
     9241 aaggaacggt aatcacgata aacatcagtt ggtaaagaaa gaacattaaa aatggaatcg
     9301 ttgcaccatg aaacatgttg gggaagcttg taacatgtct catcaaaaag aaatctgccg
     9361 ggtttccaat aacaccgccg atatcccgtc caaaggccaa tccgaatcca ccaaataccc
     9421 acataatcgt tgttatccca acggagagaa aagattgaga catgatcgtt agtatattat
     9481 tcttccgtgc taatccgccg taaaaaaaag ctagaccagg agtcatcagg caaaccattg
     9541 ctgtacataa aatcatgaat gctgtatctc ctgtattgat aggtaatgta ttcatttttt
     9601 tcgcctcctt tctacgatta cgagtataaa agaaggtgta gttgtttttt tcttctgttt
     9661 ttttgaatta atacttgcat ttataaaaga cagacaatta ataaaagctt gatgtgttgt
     9721 gctctttttc gtttttcaca caaataaaga caaaaaataa acggtcttct ttttatagaa
     9781 aaccgtttat tttcgtcttt ttttattttt aaagatattt tttcataagg aataaagaat
     9841 acttcatata aaatatataa aaaagaaccg cagcgagtgt cgctacggtt ctcaaatttg
     9901 ttcttcattt caacataagt ttttcttcaa tttgcttata atttacgtgc aaagaatgaa
     9961 acgactaata ctaaaatgat tgcaccgatc aatgatggaa tgatagccat tcctgcaatt
    10021 gttggtcccc aagttcctag taaagcttgt ccaattgctg aacctactaa accagcaatg
    10081 atgtttgcga tgatccccat tgatgatcct ttattcgtga ttgctcctgc aattgctcca
    10141 atgataccgc cgacgattaa tgcccaaata aatcccattt taaattcctc ctaactgact
    10201 tcttacttct tattgtacac gtggttcgtt attttctttg actttttgtg cacctttgcc
    10261 tacagcagat tttgctttat ctgtttggcg tgaagcaaat tgtcctgtgg attttgctgc
    10321 ttctgttact ttgtcttgta ctgtttcttg atctttttgg tattcttctt gtgttttgat
    10381 gtctgcaaca ttcacattca cttcgataac atctaaatgt gtcatattgt ttacttcagt
    10441 actgatcaat tctttgattt ggttgtaaat atcttcgatg tctttaccgt attctacgat
    10501 cacatctaga tccacagcta cttgtttctt acctacttct gtattgatac ctgctgttac
    10561 gttatctgtg ttgactaatt tttcagccat atttgagaaa aatccgccat ctaatgtcaa
    10621 tagaccatct acatctttca atgcataacc gatgattttt tggatgactt tgtcttcata
    10681 agtcaattca ccattcatca tgcggttttc tcctttttca tttgccaaaa ctgatccatt
    10741 tgttgtattc atgtttttgt tttccatctt tgtttcctcc tatgatttaa aaaattgatc
    10801 aactattcct gactgtttca aataaaaacc gattcctgcg ccaattgctg tcatgattat
    10861 gatcaacaat gtcttgaaaa atccaatcgt taaccataaa acagctagca gtagtccaag
    10921 acccccaaag ataataggga gcttgtatgc tgaaaatagt tctttcatta tttggccctc
    10981 ctattccact cgagagtgct tgtagtctct tgtatcttct tgttcttcat aaccgctgta
    11041 tgtgactgcc actttgaccg tttcttttgt tccgagtact ttagttactt cttcttcgat
    11101 atcttgcatc aatacacctg ttttccctac taatgaagaa gttcgtttca agtctccgtt
    11161 gacatggata tggattcgat ttttcgttga acgtatccgt actttaggtg aatgaatgaa
    11221 ctcttcttcg tgtaaatggg aacgtaccaa cccttcgatc gcttttttgt cgattgttag
    11281 tttgccatct tcccgtttca taacgaaagt tcctcttgac tttggataga aaagaacgac
    11341 tagcaagatg ataatagcaa gaacggcaaa tactacagct gcccagaaaa tatattgttg
    11401 catgtagtaa ttcgttaaag taaagaagcg aaatcgttcc aattgttcag gcataatata
    11461 cggcgcattg atcaacgcaa tcgctagaaa tactgaaagt agcagtaaac tcatgataac
    11521 accgattatt tttaacccct tattcatttt ccacctccta taaacttatt tttaccctgt
    11581 aaataactct accgacaatc acagtataga tagcgaacag ttagcatagc atcagtaaat
    11641 aaagcgctac caaacattgc tggatcataa cggcgtttta ttgccagtct gatactgaaa
    11701 taacaattgc ttacttattc tctatttact tttgattgct ggctatactt aaaggatatt
    11761 attcaagatt aaaaattgca aagattatgc tcagttatta ttacaagatt ctattt
//
LOCUS       DADVXO010000089        11495 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.092, whole genome shotgun sequence.
ACCESSION   DADVXO010000089 DADVXO010000000
VERSION     DADVXO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.092"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(207..692)
                     /locus_tag="KB950_002035"
     CDS             complement(207..692)
                     /locus_tag="KB950_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBB6858248.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    782..1127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1172..1813
                     /locus_tag="KB950_002036"
     CDS             1172..1813
                     /locus_tag="KB950_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6858249.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1832..2467
                     /locus_tag="KB950_002037"
     CDS             1832..2467
                     /locus_tag="KB950_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6858250.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2485..3300
                     /locus_tag="KB950_002038"
     CDS             2485..3300
                     /locus_tag="KB950_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6858251.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3619..5055
                     /locus_tag="KB950_002039"
     CDS             3619..5055
                     /locus_tag="KB950_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6858252.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5244..7676
                     /locus_tag="KB950_002040"
     CDS             5244..7676
                     /locus_tag="KB950_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBB6858253.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7906..8973
                     /gene="rlmN"
                     /locus_tag="KB950_002041"
     CDS             7906..8973
                     /gene="rlmN"
                     /locus_tag="KB950_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBB6858254.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9166..10269)
                     /locus_tag="KB950_002042"
     CDS             complement(9166..10269)
                     /locus_tag="KB950_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6858255.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10821..11369
                     /gene="aac(6')"
                     /locus_tag="KB950_002043"
     CDS             10821..11369
                     /gene="aac(6')"
                     /locus_tag="KB950_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBB6858256.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3814 a         1943 c         2479 g         3259 t
ORIGIN      
        1 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
       61 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
      121 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
      181 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
      241 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
      301 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
      361 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
      421 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
      481 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
      541 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
      601 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
      661 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
      721 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
      781 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
      841 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
      901 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
      961 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     1021 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     1081 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     1141 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     1201 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     1261 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     1321 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     1381 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     1441 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     1501 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     1561 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     1621 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     1681 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     1741 ctatcttaca ttgactggta ttctaacaat cgtaatgaaa caagttgaag gaaaattaaa
     1801 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     1861 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     1921 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     1981 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     2041 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     2101 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     2161 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     2221 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     2281 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     2341 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     2401 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     2461 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     2521 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     2581 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     2641 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     2701 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     2761 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     2821 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     2881 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     2941 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     3001 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     3061 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     3121 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     3181 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     3241 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     3301 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     3361 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     3421 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     3481 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     3541 attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     3601 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     3661 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     3721 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     3781 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     3841 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     3901 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     3961 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     4021 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     4081 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     4141 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     4201 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     4261 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     4321 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     4381 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     4441 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     4501 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     4561 ggtgctggaa aaatgaatac gacaggaaaa aaaagttcat cagaagaaac tggagttcct
     4621 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     4681 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     4741 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     4801 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     4861 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     4921 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     4981 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     5041 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     5101 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     5161 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     5221 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     5281 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     5341 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     5401 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     5461 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     5521 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     5581 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     5641 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     5701 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     5761 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     5821 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     5881 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     5941 ggcgcttagc tgcaaaagaa gctgcagaat atgagaagaa aaaagcggtg agccagcaaa
     6001 gggaaaatac tgctgatgaa tggcaggaac cagaaaatac agagccggag cagctaagtt
     6061 ttgttccaat cgacagtttt caagaaaata tccatcctgc gaatctagag aaaccagtac
     6121 cagatacacc aaaacagacc aatacagcag aaggtttttc agatgagttg ccagaggatg
     6181 acggtacctc attagaattt gagattgaag cagaacagga aaatcaagac tatgaattac
     6241 cgacagttga tttgcttgat tctataccaa ctgtggatca aagtgatgag tataaaaaaa
     6301 ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg
     6361 tcgtcaaagc cagcttagga ccatccgtca caaaattcga agtccagccg gctgtagggg
     6421 taaaagtaag taaaattgtg aacttaacag atgatatcgc gcttgcttta gcggcgaaag
     6481 atgtccggat ggaagcaccg attccaggta aatcactgat tggtattgaa gtaccaaatg
     6541 gaaagatcag tatggtttcc tttagagaaa tcatcgaagc acaaccaaac cacccagata
     6601 aattactaga agtaccttta ggacgtgatg tttctggacg ggtacaaacg gctgatcttt
     6661 cgaagatgcc tcacttactc gtggctggtt cgactggtag tgggaagtca gtagcaatta
     6721 atgggattat cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga
     6781 tcgatccgaa aatggttgaa ttgaatatgt acaacggcat acctcatctg ttgacgccgg
     6841 tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag
     6901 aacgatatga aaaattcgcg gcaaccggag taagaaatat ttctggttac aatgaatttg
     6961 ttcaacagaa aaacttggaa aacggaacga aacatccaac cttaccattc atcgtagtga
     7021 tcgtagatga attagctgat ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta
     7081 ttcgtttagc gcaaatggct cgggcagcgg ggattcatat gatcctagct actcaacgtc
     7141 caagtgtgga tgttatcaca ggaatcatca aagcaaatgt accttccaga atggcatttg
     7201 cggtatcaag cggtacagat tctcggacga tcattgattc taatggggca gaaaagctct
     7261 tagggcgagg agatatgcta ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag
     7321 gagcattcat ttctgaccac gaagtagaac gggtcgttca atttgttaca gatcagcaag
     7381 aagctcatta tgaagagaaa atgatgccga cagatgaagt tgaaacgaca ggtgctccag
     7441 aacagccaca agatgagctg tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg
     7501 caagtatttc ccttctccaa agaagatttc ggatcggtta caaccgagca gcacgtttag
     7561 tagatgaact ggaagctcat ggagttgttg gcccgtcaga aggaagcaag cctcggaaag
     7621 tattcattga gcaggaagaa acatcagaaa cgttgccgga cgataatgac atgtaaaact
     7681 tgtgtgatga agggttgaaa aacgaataac caataaaaag acaaggcatg atgataccaa
     7741 aagaaaaaat ggtttttttc catcttttct aatacggtat gatgtcttgt ctttttagtt
     7801 tttgtgagat taaagttcta ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc
     7861 atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa atactttgga aaaacagtcc
     7921 atttatggct tgacgaatga agaattaatc aattggttta tagaaaacgg cgagaaaaaa
     7981 ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa
     8041 atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat catcaatcct
     8101 ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt gtttgaactc
     8161 cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt
     8221 gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa
     8281 aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat
     8341 tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag
     8401 ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga
     8461 ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag
     8521 gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat
     8581 gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa
     8641 gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg
     8701 aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag
     8761 aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat
     8821 tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta
     8881 aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga
     8941 agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac
     9001 cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc
     9061 tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt
     9121 cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt
     9181 atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt
     9241 aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt
     9301 atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg
     9361 aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat
     9421 catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt
     9481 ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt
     9541 aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc
     9601 atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg
     9661 atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc
     9721 acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa
     9781 gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt
     9841 tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt
     9901 gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc
     9961 accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc
    10021 tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc
    10081 gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc
    10141 tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac
    10201 gattccattt gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact
    10261 ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat
    10321 cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat
    10381 aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa
    10441 acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg
    10501 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
    10561 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
    10621 acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt
    10681 tctgtataat tttttatttt ataagtaaga taaataatga aagaatagag atactgattg
    10741 gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc
    10801 aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga
    10861 aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg
    10921 cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag
    10981 atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc
    11041 atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt
    11101 acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt
    11161 tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg
    11221 cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa
    11281 tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa
    11341 cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct
    11401 tcaagaaata agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt
    11461 atttccgaag aagctacttt tggaacaccg tgtac
//
LOCUS       DADVXO010000090        10847 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.093, whole genome shotgun sequence.
ACCESSION   DADVXO010000090 DADVXO010000000
VERSION     DADVXO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.093"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(5..1810)
                     /gene="glmS"
                     /locus_tag="KB950_002044"
     CDS             complement(5..1810)
                     /gene="glmS"
                     /locus_tag="KB950_002044"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBB6858257.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2219..2509
                     /locus_tag="KB950_002045"
     CDS             2219..2509
                     /locus_tag="KB950_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858258.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2522..3547
                     /gene="sppA"
                     /locus_tag="KB950_002046"
     CDS             2522..3547
                     /gene="sppA"
                     /locus_tag="KB950_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBB6858259.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3550..4209
                     /locus_tag="KB950_002047"
     CDS             3550..4209
                     /locus_tag="KB950_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBB6858260.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4366..5298
                     /locus_tag="KB950_002048"
     CDS             4366..5298
                     /locus_tag="KB950_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858261.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5452..6741
                     /locus_tag="KB950_002049"
     CDS             5452..6741
                     /locus_tag="KB950_002049"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBB6858262.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6988..8238
                     /gene="clpX"
                     /locus_tag="KB950_002050"
     CDS             6988..8238
                     /gene="clpX"
                     /locus_tag="KB950_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBB6858263.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALSAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8321..8908
                     /locus_tag="KB950_002051"
     CDS             8321..8908
                     /locus_tag="KB950_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBB6858264.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8982..9137)
                     /locus_tag="KB950_002052"
     CDS             complement(8982..9137)
                     /locus_tag="KB950_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858265.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            9273..10600
                     /locus_tag="KB950_002053"
                     /pseudo
     CDS             9273..10600
                     /locus_tag="KB950_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10611..10820
                     /locus_tag="KB950_002054"
     CDS             10611..10820
                     /locus_tag="KB950_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858266.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         3605 a         1874 c         2243 g         3125 t
ORIGIN      
        1 tttattattc tactgtcact gatttcgcta aattacgtgg tttgtcaacg tcgaatcccc
       61 gtaatagcgt tgcgtaatat gcaagtaatt gagtcggtac gactgtggtt agagtagtta
      121 gataagggtg gacagttggt agcaccagct ggtcactttc cttttctaat ccctctgaga
      181 caataactag tgttttagcg ccacggcttt cgacttcttt caggtttcct cgagtatgcg
      241 caccagtcac ttcttcactg atgattccga tcactggtgt tccttcttcg atcaaagcaa
      301 tcgttccatg tttcaattcg ccagcggcaa aaccttctgc ctggatataa ctgatttctt
      361 tcaatttcaa agcagcttca agagaaacgt tgtagtcagc accacgtcca atgtaaaagg
      421 catttcttgt atcgctgaga tatacttctg ccaactcttc taaataatct ttttgggcga
      481 tgaccgattc catcgcatta gcaactaaac tcaattcatg agtgatatcg aatacaagag
      541 aagtttcaaa ttctttttta tcaccgatag ctttggctaa taaagttagt acagctattt
      601 gtgcagtata agctttcgtt gatgctactg cgatttcggg tccagcatgc aatagcagtg
      661 taaaggatgc ttcccgcgaa agagtagaac cagcaacatt tgtaatcgta agtgaagggt
      721 agccgagacg gttgattttt accagcactt ggcggctgtc tgctgtttcg ccactttgac
      781 tcaagaaaat aaagaatggt ttttttgaaa gaagaggtgt gttatagcca aattcactgg
      841 ctaaatgaac ctcaacagga atctgtgtca atttttccaa agtctgttta cctgctaacc
      901 ccgcatggta actagttccg caagcaacga tatagattcg atcgctatca gacaatgtat
      961 cgaccagctt ttgatcaaga gagacacgat tgtcttctgt tacgtatttt tgagcgatac
     1021 gacgcatgat aataggctgt tcgtcgattt ctttcagcat ataaaaagga taggtacctt
     1081 tctcgatatc tgaagcatct acttgtgctt cgaatgaaga acgttctacg atgtcacctg
     1141 caaaattttg aataataact ttttctgatg tgacggtaac catctcttca tcttcaatct
     1201 caacgaattc cttcgtatga gcaagcatag ccattgcatc gctggcaatc acattgaatc
     1261 catcgccgag tccgattaat agtggacttt tatttttagc gacgtaaata acatcaggtg
     1321 ctgtccgatc aatcaaagcg aaagcgtaag aacctttgat gacccgcaaa gcttttaaaa
     1381 acgcctcttg cgttgtagta tccttcgcga aagtttcaat tagatgagcg ataatctcag
     1441 tatcggtctc accaataaag tggtcatttg ctaaataagc aattttaagt tcttcaaagt
     1501 tttcgattac gccgttgtga acgagcacga agcgtccact ttgggaagta tgtggatggg
     1561 cgttttcttc agaaggttgc ccgtgagttg cccatcgtgt atgaccaata cctatatttc
     1621 ctgtagtatc aatatttatt ttgtttttca aattttgaat tctgccttgt gctttgacca
     1681 gataatcagt tgtgccatct gagacaaaga taccagctga atcataacca cggtattcta
     1741 atttttctag accgtcaatc aatcctggtg ttacattttt taatccaatc attccaacaa
     1801 ttccgcacat aaaaaacatc ctactttcta taatgaattc gtatgaatgc ttccagctgc
     1861 aggaaaatcc ttttgtttta tctttgcaga cattttttgt ggattttctg tagagtgcag
     1921 tccctagggc catccgccga atctttcgat aaaccctctc ctcgtcatct gtttacacag
     1981 acctggcgct atcctaaaag gtataactat cctccttttt gttttttatc tggaaacatt
     2041 taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa aataaattgg
     2101 tatatatcaa attctttgtc aaatgtcctt tttatgtcaa aaaataatgg gtttttatct
     2161 atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag gagtaaaaat
     2221 ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt ccaaattcga
     2281 agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct gggatagcat
     2341 ccaagaaatc gcacctgatg ccttggaagt tgctcaagct cgtgagttta cggtagaaga
     2401 tgaattaaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag agcggttcga
     2461 cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag
     2521 aatgaataaa agacgttggg cagcagttct gattgctgca ggattatttg tggtttcgtt
     2581 agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat tgaacagttg
     2641 gttatatggg gatgaagaac tagctccaaa tgtgttggaa gagggagaca gtacgcaaaa
     2701 aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag gtttatttgc
     2761 ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg aagaagatcc
     2821 aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc
     2881 ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa tgtatgtcag
     2941 catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag ataaaatctt
     3001 tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat tgaattattc
     3061 aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga
     3121 tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc aagcatatat
     3181 cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata aatctgaaga
     3241 agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa aagaagtagg
     3301 tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatacgaa aggaacaaca
     3361 gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg ctagtacatg
     3421 gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta gtcagatctt
     3481 atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt attacggagg
     3541 tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga taagcaacag
     3601 cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc ttttgaaaga
     3661 gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa gtatacggac
     3721 acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc aggttttttt
     3781 atccgaatgt ttgccttctt ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca
     3841 cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct gagtctctac
     3901 ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa attcaatcat
     3961 ggccaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga tgaagaagaa
     4021 cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct aaaatttcct
     4081 ttgctgatgt tagggtatct tccagcagtc tttaatcaga aaaagcaaca tctgggcgat
     4141 tttttcacta atacaagtgt tgttacgatc aatctgatca aagcgttcaa ccaggaagtc
     4201 aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa agtcagatct
     4261 aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt ttttgttttg
     4321 ctactattga acatgagctg aatatgagca agaggaggac actgcgtgaa gccgattgat
     4381 tttccagaaa aatatgagaa tttaatgcga gtggcacaac aagcactagc aaaccagcaa
     4441 tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag ctttgaggct
     4501 aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc attgaagcaa
     4561 gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt ttattttgac
     4621 ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag tacagatttt
     4681 tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga attatccgga
     4741 caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact tccttcgttg
     4801 gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta tcatgagttt
     4861 atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc aagggcaaaa
     4921 ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt gacgattgaa
     4981 gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca ggcaagttac
     5041 cagcaattat gtgtattttc agatcgttac gggaatgaag atgcgctgct tagttccgtg
     5101 ttaaaagaag aattcacgtt gcaatctgca attgtttatc ctgtctatga tacatacatc
     5161 gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa aacgatgtat
     5221 gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa
     5281 atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg aaaaacaagg
     5341 attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt aaaggtatgc
     5401 atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta aatgactgca
     5461 acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc gcaagacgaa
     5521 atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga
     5581 ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga agaagcttta
     5641 tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt caaagaagct
     5701 ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac
     5761 tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca atataaagat
     5821 ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc acgtatcaaa
     5881 cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc tgaaaatggc
     5941 gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa
     6001 ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt tgaagatcaa
     6061 ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc agaagattat
     6121 caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa
     6181 gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga cgaagtatca
     6241 acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa agaaaaagca
     6301 gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt
     6361 gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga attcttgaac
     6421 aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg ttctacagaa
     6481 gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg
     6541 atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat caatgcagaa
     6601 atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca
     6661 gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca
     6721 gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa gctacttcta
     6781 gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt ccttattttt
     6841 tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa cactttttct
     6901 ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt ttcttgcata
     6961 ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa tgagaccgta
     7021 cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg
     7081 gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga agaattttat
     7141 gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct tgaagtattg
     7201 aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc cgtatacaat
     7261 cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga actgcaaaaa
     7321 agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc acaaacatta
     7381 gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac agaagctggt
     7441 tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc tgattacaat
     7501 gtggaacttg cagaaaaagg gatcatctat attgatgaga tcgataaaat tgcgcgtaaa
     7561 agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg
     7621 aaaattctag aaggaactgt tgcaagtgtg ccacctcagg gcggacgtaa acatccacat
     7681 caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg tgcatttgat
     7741 ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa
     7801 aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga agatttacta
     7861 aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc tttagagaaa
     7921 ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct agtcaaacaa
     7981 tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga agcgttgagt
     8041 gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg ttccatcatt
     8101 gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg
     8161 attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta taaagattca
     8221 gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc ttttgcgaca
     8281 gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc atcaagctga
     8341 aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc cagaaatagc
     8401 tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga ttgatcgaaa
     8461 gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt tttatttgat
     8521 cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag tatccaaaac
     8581 tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag accagctacg
     8641 tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata tccagatgta
     8701 tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg cagataagat
     8761 ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg attttgatcg
     8821 caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc
     8881 agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca gtcaaactct
     8941 ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg tttcttgtct
     9001 tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc ttttggtgca
     9061 actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt atctgtacgt
     9121 tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc tatcatgtaa
     9181 tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcatt ggttataatt
     9241 aaattataaa aagatttgaa agaggtgaga aaatgattgg cgaagataaa ttattgatga
     9301 ctatccagct cacagactta aatgaagaag aaaaaaacag gaaataagcg attgggctat
     9361 tttgacacgt ctgttgatcc agccaacatt tatccgctct ttcacacaac aagcggatcc
     9421 gtatgatctt cgaaagcttc aagaaaaaat aatgctagca tcggtaagag acgaaagttg
     9481 gttagttgta ggaaatgacg agaacgaatg tagttttcgc ttggaagata atcaattatt
     9541 gataaaaaat gtattgtcta tctctgtttt caaagaaaag caatttttga ttcgagactt
     9601 cattcagaaa aaaatgattg ctcatggggt gttcgcctat atgcgagctt atagcgagtt
     9661 tatctatcac aatacaaaac agatctctca acggctattg tttgagaaaa aggatgaaat
     9721 cgaacattta cctaaaatga aacaacaaaa cggggaagta gtagtcgatt gcaaccaatt
     9781 tccaggatac gatctttttt accaaggttt atgtttcact tcgtgttggg aaatgtatta
     9841 tagtcgctat tatcatcaaa tcatccctaa accgattttt ctcgacatcc aacaagtaga
     9901 aaaagtgaag gaattagaaa acgaagtcat atgtatccag ctttatcgag acccctttaa
     9961 ctggcaaaag ccaaacaatc aaatgtttca atcctatttt agggaccagc ttgggtttga
    10021 tcatcttgct tgggacaatg gtgtcggttt attgaaaccg ccattcgttg aatacattta
    10081 cactgaccat acgatccaat ctgtccagta tcagaatgca caaatgcaac ctgttcctaa
    10141 aaagaatgct tcattttttg taacaaaaag ttatgatatc caacaaggag attataaaga
    10201 aaggcgtgtt cgcggaacac taaatgctca ggcgtatttt ccttgggttg acgagaatcg
    10261 atcacgcatg atgtgctata aagttatcga tccgactgtt gctttggata atggaatcga
    10321 agcttattgt tattatatta gggaatatct agaagtagag gtaacagatg aaaaatatca
    10381 agagtatctt ttatccttaa gaatctatgt tccttcagaa tccctgtcag aattaccgtt
    10441 aaaggaaatc aaacatagac tctctgatgt taccttcaaa cgaataaaga aaaagcggcg
    10501 aagtatccaa ttcgatgtca agaaaggtga gcagcatctg cgtgtgcaat ttttagatta
    10561 ccgagaatta gaacaattga ttactttaca aaaaatatga ggtgaagaaa atggataaac
    10621 aactctatta tcgaataatg gttccggcat cattattatc cgtctgtctc ttttttgttc
    10681 ctaattttcc tattcgatca gtcatcggtt ttttggtcat gtttggcggg atgggacttt
    10741 attattatct tcttcataag gaaagaaaac ctgcttctgt ttatatgaaa aaaacaagaa
    10801 gcgaaaataa aaaacgataa aacggcttga tgatagacaa aaaaata
//
LOCUS       DADVXO010000091        10803 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.094, whole genome shotgun sequence.
ACCESSION   DADVXO010000091 DADVXO010000000
VERSION     DADVXO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.094"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            12..84
                     /locus_tag="KB950_002055"
     tRNA            12..84
                     /locus_tag="KB950_002055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="KB950_002056"
     tRNA            88..160
                     /locus_tag="KB950_002056"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="KB950_002057"
     tRNA            168..249
                     /locus_tag="KB950_002057"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="KB950_002058"
     tRNA            267..339
                     /locus_tag="KB950_002058"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="KB950_002059"
     tRNA            351..422
                     /locus_tag="KB950_002059"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="KB950_002060"
     tRNA            439..524
                     /locus_tag="KB950_002060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="KB950_002061"
     tRNA            535..608
                     /locus_tag="KB950_002061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="KB950_002062"
     tRNA            618..691
                     /locus_tag="KB950_002062"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="KB950_002063"
     tRNA            711..783
                     /locus_tag="KB950_002063"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="KB950_002064"
     tRNA            811..884
                     /locus_tag="KB950_002064"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="KB950_002065"
     tRNA            898..971
                     /locus_tag="KB950_002065"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="KB950_002066"
     tRNA            995..1084
                     /locus_tag="KB950_002066"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="KB950_002067"
     tRNA            1097..1170
                     /locus_tag="KB950_002067"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="KB950_002068"
     tRNA            1189..1264
                     /locus_tag="KB950_002068"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="KB950_002069"
     tRNA            1266..1336
                     /locus_tag="KB950_002069"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="KB950_002070"
     tRNA            1350..1423
                     /locus_tag="KB950_002070"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="KB950_002071"
     tRNA            1448..1535
                     /locus_tag="KB950_002071"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="KB950_002072"
     tRNA            1557..1628
                     /locus_tag="KB950_002072"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="KB950_002073"
     tRNA            1651..1740
                     /locus_tag="KB950_002073"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="KB950_002074"
     tRNA            1753..1826
                     /locus_tag="KB950_002074"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="KB950_002075"
     tRNA            1845..1917
                     /locus_tag="KB950_002075"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="KB950_002076"
     tRNA            1921..1993
                     /locus_tag="KB950_002076"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="KB950_002077"
     tRNA            1998..2068
                     /locus_tag="KB950_002077"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="KB950_002078"
     tRNA            2081..2154
                     /locus_tag="KB950_002078"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="KB950_002079"
     tRNA            2166..2237
                     /locus_tag="KB950_002079"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="KB950_002080"
     CDS             2503..3696
                     /locus_tag="KB950_002080"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBB6858267.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="KB950_002081"
     CDS             3826..5298
                     /locus_tag="KB950_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6858268.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="KB950_002082"
     CDS             5381..6169
                     /locus_tag="KB950_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBB6858269.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="KB950_002083"
     CDS             complement(6236..6904)
                     /locus_tag="KB950_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBB6858270.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="KB950_002084"
     CDS             7019..8731
                     /locus_tag="KB950_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858271.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="KB950_002085"
     CDS             8734..10506
                     /locus_tag="KB950_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858272.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10803)
                     /locus_tag="KB950_002086"
     CDS             complement(10588..>10803)
                     /locus_tag="KB950_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6858273.1"
                     /translation="AVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDS
                     KTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3097 a         1955 c         2493 g         3258 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgc
//
LOCUS       DADVXO010000092        10745 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.095, whole genome shotgun sequence.
ACCESSION   DADVXO010000092 DADVXO010000000
VERSION     DADVXO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.095"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(264..1112)
                     /locus_tag="KB950_002087"
     CDS             complement(264..1112)
                     /locus_tag="KB950_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6858274.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1111..2178
                     /gene="gnd"
                     /locus_tag="KB950_002088"
     CDS             1111..2178
                     /gene="gnd"
                     /locus_tag="KB950_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBB6858275.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2180..3181
                     /locus_tag="KB950_002089"
     CDS             2180..3181
                     /locus_tag="KB950_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBB6858276.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3178..3462
                     /locus_tag="KB950_002090"
     CDS             3178..3462
                     /locus_tag="KB950_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBB6858277.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3495..4292
                     /locus_tag="KB950_002091"
     CDS             3495..4292
                     /locus_tag="KB950_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6858278.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4306..5139
                     /locus_tag="KB950_002092"
     CDS             4306..5139
                     /locus_tag="KB950_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858279.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5157..6014
                     /locus_tag="KB950_002093"
     CDS             5157..6014
                     /locus_tag="KB950_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6858280.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6036..6545
                     /locus_tag="KB950_002094"
     CDS             6036..6545
                     /locus_tag="KB950_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858281.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6582..6983
                     /locus_tag="KB950_002095"
     CDS             6582..6983
                     /locus_tag="KB950_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858282.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7095..7448
                     /locus_tag="KB950_002096"
     CDS             7095..7448
                     /locus_tag="KB950_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858283.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7482..7925
                     /locus_tag="KB950_002097"
     CDS             7482..7925
                     /locus_tag="KB950_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6858284.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7942..9027
                     /gene="uxuA"
                     /locus_tag="KB950_002098"
     CDS             7942..9027
                     /gene="uxuA"
                     /locus_tag="KB950_002098"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6858285.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9020..9733
                     /locus_tag="KB950_002099"
     CDS             9020..9733
                     /locus_tag="KB950_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6858286.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9940..10089)
                     /locus_tag="KB950_002100"
     CDS             complement(9940..10089)
                     /locus_tag="KB950_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858287.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10485..10685
                     /locus_tag="KB950_002101"
     CDS             10485..10685
                     /locus_tag="KB950_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6858288.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3601 a         1695 c         2320 g         3129 t
ORIGIN      
        1 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
       61 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
      121 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
      181 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
      241 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
      301 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
      361 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
      421 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
      481 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
      541 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
      601 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
      661 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
      721 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
      781 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
      841 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
      901 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
      961 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
     1021 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
     1081 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
     1141 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
     1201 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
     1261 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
     1321 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
     1381 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
     1441 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
     1501 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
     1561 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
     1621 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
     1681 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
     1741 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
     1801 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
     1861 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
     1921 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
     1981 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
     2041 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
     2101 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
     2161 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
     2221 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
     2281 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
     2341 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
     2401 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
     2461 acaccagaca atcgtatcta tcatacctgc tttaattgaa cgaaatgctg tagcagaaaa
     2521 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
     2581 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
     2641 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
     2701 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
     2761 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
     2821 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
     2881 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
     2941 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
     3001 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
     3061 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
     3121 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
     3181 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
     3241 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
     3301 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
     3361 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
     3421 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
     3481 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
     3541 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
     3601 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
     3661 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
     3721 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
     3781 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
     3841 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
     3901 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgcgg
     3961 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
     4021 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
     4081 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
     4141 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
     4201 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
     4261 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
     4321 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
     4381 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
     4441 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
     4501 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
     4561 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
     4621 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
     4681 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
     4741 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
     4801 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
     4861 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
     4921 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
     4981 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
     5041 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
     5101 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
     5161 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
     5221 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
     5281 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
     5341 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
     5401 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
     5461 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
     5521 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
     5581 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
     5641 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
     5701 gccgtaatgc tacaggccta atgcaaaaca ctggtgttca gacagcgatt gaaatgctat
     5761 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
     5821 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
     5881 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
     5941 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
     6001 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
     6061 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
     6121 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
     6181 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
     6241 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
     6301 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
     6361 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
     6421 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
     6481 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
     6541 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
     6601 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
     6661 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
     6721 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
     6781 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
     6841 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
     6901 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
     6961 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
     7021 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
     7081 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
     7141 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
     7201 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
     7261 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
     7321 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
     7381 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
     7441 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
     7501 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
     7561 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
     7621 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
     7681 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
     7741 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
     7801 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
     7861 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
     7921 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
     7981 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
     8041 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
     8101 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
     8161 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
     8221 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
     8281 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
     8341 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
     8401 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
     8461 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
     8521 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
     8581 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
     8641 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
     8701 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
     8761 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
     8821 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
     8881 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
     8941 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
     9001 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
     9061 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
     9121 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
     9181 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
     9241 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
     9301 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
     9361 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
     9421 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
     9481 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
     9541 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
     9601 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
     9661 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
     9721 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
     9781 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
     9841 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
     9901 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
     9961 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    10021 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    10081 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    10141 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    10201 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    10261 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt
    10321 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    10381 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    10441 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    10501 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    10561 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    10621 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    10681 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agacggtttt tttgtacgaa
    10741 aaaat
//
LOCUS       DADVXO010000093        10616 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.096, whole genome shotgun sequence.
ACCESSION   DADVXO010000093 DADVXO010000000
VERSION     DADVXO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.096"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            33..656
                     /locus_tag="KB950_002102"
     CDS             33..656
                     /locus_tag="KB950_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858289.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="KB950_002103"
     CDS             1106..2155
                     /locus_tag="KB950_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6858290.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="KB950_002104"
     CDS             2152..3033
                     /locus_tag="KB950_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858291.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="KB950_002105"
     CDS             3036..3806
                     /locus_tag="KB950_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6858292.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="KB950_002106"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="KB950_002106"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBB6858293.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="KB950_002107"
     CDS             5117..6280
                     /locus_tag="KB950_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6858294.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="KB950_002108"
     CDS             6397..6807
                     /locus_tag="KB950_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6858295.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="KB950_002109"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="KB950_002109"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6858296.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="KB950_002110"
     CDS             9380..9664
                     /locus_tag="KB950_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858297.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="KB950_002111"
     CDS             9824..10060
                     /locus_tag="KB950_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6858298.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="KB950_002112"
     CDS             10057..10455
                     /locus_tag="KB950_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBB6858299.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3712 a         1771 c         2242 g         2891 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatag
//
LOCUS       DADVXO010000094        10097 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.097, whole genome shotgun sequence.
ACCESSION   DADVXO010000094 DADVXO010000000
VERSION     DADVXO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.097"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            276..545
                     /gene="rpsN"
                     /locus_tag="KB950_002113"
     CDS             276..545
                     /gene="rpsN"
                     /locus_tag="KB950_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBB6858300.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            560..739
                     /gene="rpmF"
                     /locus_tag="KB950_002114"
     CDS             560..739
                     /gene="rpmF"
                     /locus_tag="KB950_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBB6858301.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            771..1148
                     /locus_tag="KB950_002115"
     CDS             771..1148
                     /locus_tag="KB950_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858302.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1169..1318
                     /gene="rpmG"
                     /locus_tag="KB950_002116"
     CDS             1169..1318
                     /gene="rpmG"
                     /locus_tag="KB950_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6858303.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1342..2325
                     /locus_tag="KB950_002117"
     CDS             1342..2325
                     /locus_tag="KB950_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBB6858304.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2355..2477
                     /locus_tag="KB950_002118"
     CDS             2355..2477
                     /locus_tag="KB950_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBB6858305.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2495..4018
                     /locus_tag="KB950_002119"
     CDS             2495..4018
                     /locus_tag="KB950_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBB6858306.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4042..4281
                     /locus_tag="KB950_002120"
     CDS             4042..4281
                     /locus_tag="KB950_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858307.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4474..4935)
                     /locus_tag="KB950_002121"
     CDS             complement(4474..4935)
                     /locus_tag="KB950_002121"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6858308.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5130..5348)
                     /locus_tag="KB950_002122"
     CDS             complement(5130..5348)
                     /locus_tag="KB950_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858309.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5466..7082
                     /locus_tag="KB950_002123"
     CDS             5466..7082
                     /locus_tag="KB950_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBB6858310.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7323..8663)
                     /locus_tag="KB950_002124"
                     /pseudo
     CDS             complement(7323..8663)
                     /locus_tag="KB950_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8939..9094)
                     /locus_tag="KB950_002125"
     CDS             complement(8939..9094)
                     /locus_tag="KB950_002125"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBB6858311.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9390..9722)
                     /locus_tag="KB950_002126"
     CDS             complement(9390..9722)
                     /locus_tag="KB950_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6858312.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3359 a         1602 c         1998 g         3138 t
ORIGIN      
        1 tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct
       61 tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga
      121 taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt
      181 gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag
      241 gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta
      301 aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag
      361 ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga
      421 ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc
      481 gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt
      541 ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg
      601 gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg
      661 tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat
      721 taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta
      781 tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc
      841 tagataaatg cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt
      901 taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct
      961 tatcatttga tgatttttta agaatctatc caatttttga agagtatgac gaggagttat
     1021 ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac
     1081 aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag
     1141 atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga
     1201 aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga
     1261 attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct
     1321 aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg
     1381 gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa
     1441 attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat
     1501 agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat
     1561 ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca
     1621 caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca aacactttta
     1681 acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt
     1741 gacagttttg aacttattga aagagaacca caagccctta agcaacttgt catggcagat
     1801 cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga
     1861 gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt
     1921 caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat
     1981 tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt
     2041 aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt
     2101 gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta
     2161 caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac
     2221 accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa
     2281 aggatcaaac agagttttga taaattactg gcggaaagca aataatgata agtagtgatt
     2341 gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc
     2401 cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa
     2461 tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat
     2521 tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc
     2581 gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac
     2641 caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc
     2701 tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt
     2761 atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc
     2821 tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga
     2881 agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc
     2941 acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc
     3001 gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt
     3061 ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca
     3121 cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact
     3181 aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa
     3241 aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt
     3301 ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga
     3361 acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga
     3421 aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa
     3481 gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta
     3541 tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt
     3601 cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac
     3661 tactggctat aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt
     3721 agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta
     3781 tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg
     3841 ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca
     3901 tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac
     3961 tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa
     4021 ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat
     4081 attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta
     4141 gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg
     4201 gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg
     4261 cataatagct tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga
     4321 tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga
     4381 agacttcata aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa
     4441 tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt
     4501 ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg
     4561 aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt
     4621 tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat
     4681 cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa
     4741 gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat
     4801 atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat
     4861 cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt
     4921 tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc
     4981 gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg
     5041 ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg
     5101 acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct
     5161 tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc
     5221 agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa
     5281 aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac
     5341 tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc
     5401 gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt
     5461 tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga
     5521 tacttctaaa tcatattctg tacggtgatc acggaaagaa aattgctgtc attgtaaatg
     5581 acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg
     5641 gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga
     5701 ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt
     5761 caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag
     5821 acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac
     5881 gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg
     5941 tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca
     6001 tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc
     6061 taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact
     6121 tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct
     6181 ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta
     6241 tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta
     6301 cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac
     6361 atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa
     6421 attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg
     6481 aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg
     6541 gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc
     6601 ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta
     6661 gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa
     6721 ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg
     6781 agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa
     6841 tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg
     6901 gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc
     6961 ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt
     7021 taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact
     7081 aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca
     7141 ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc
     7201 acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt
     7261 tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac
     7321 ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta
     7381 ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
     7441 aagtttctat agccataggc aattcgtttt agtactttga tatggttatt caaacattct
     7501 atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg
     7561 aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc
     7621 gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg
     7681 tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc
     7741 agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc
     7801 ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
     7861 gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga
     7921 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
     7981 gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag
     8041 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
     8101 ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca
     8161 tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg
     8221 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata
     8281 gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag
     8341 cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     8401 cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa taagatggat
     8461 acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat
     8521 tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc
     8581 tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct
     8641 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     8701 ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     8761 cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc
     8821 aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat
     8881 cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg attaattctc
     8941 atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc
     9001 tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct
     9061 ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac
     9121 ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg
     9181 ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat
     9241 ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac
     9301 catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt
     9361 gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc
     9421 atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac
     9481 tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca
     9541 ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc
     9601 catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt ggtagttact
     9661 tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc
     9721 aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca
     9781 acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa
     9841 ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat
     9901 aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct
     9961 cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa
    10021 agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac gaattaaaaa
    10081 acgttgataa ttaaaat
//
LOCUS       DADVXO010000095        10084 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.098, whole genome shotgun sequence.
ACCESSION   DADVXO010000095 DADVXO010000000
VERSION     DADVXO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.098"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(109..2229)
                     /locus_tag="KB950_002127"
     CDS             complement(109..2229)
                     /locus_tag="KB950_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6858313.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="KB950_002128"
     CDS             2459..3637
                     /locus_tag="KB950_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBB6858314.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="KB950_002129"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="KB950_002129"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBB6858315.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="KB950_002130"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="KB950_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBB6858316.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="KB950_002131"
     CDS             complement(4990..6243)
                     /locus_tag="KB950_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6858317.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="KB950_002132"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="KB950_002132"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBB6858318.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="KB950_002133"
     CDS             7821..9167
                     /locus_tag="KB950_002133"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBB6858319.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="KB950_002134"
     CDS             complement(9475..9861)
                     /locus_tag="KB950_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6858320.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10084)
                     /locus_tag="KB950_002135"
     CDS             complement(9910..>10084)
                     /locus_tag="KB950_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBB6858321.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3215 a         1839 c         2053 g         2977 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt atttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgtt
//
LOCUS       DADVXO010000096         9455 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.099, whole genome shotgun sequence.
ACCESSION   DADVXO010000096 DADVXO010000000
VERSION     DADVXO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.099"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(130..777)
                     /locus_tag="KB950_002136"
     CDS             complement(130..777)
                     /locus_tag="KB950_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBB6858322.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1056..1559)
                     /locus_tag="KB950_002137"
     CDS             complement(1056..1559)
                     /locus_tag="KB950_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6858323.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1682..2446)
                     /locus_tag="KB950_002138"
     CDS             complement(1682..2446)
                     /locus_tag="KB950_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBB6858324.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2553..2828
                     /locus_tag="KB950_002139"
     CDS             2553..2828
                     /locus_tag="KB950_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBB6858325.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3505..4455
                     /gene="yidC"
                     /locus_tag="KB950_002140"
     CDS             3505..4455
                     /gene="yidC"
                     /locus_tag="KB950_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBB6858326.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="KB950_002141"
     CDS             complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="KB950_002141"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBB6858327.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5605..6063)
                     /locus_tag="KB950_002142"
     CDS             complement(5605..6063)
                     /locus_tag="KB950_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6858328.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6060..7523)
                     /locus_tag="KB950_002143"
     CDS             complement(6060..7523)
                     /locus_tag="KB950_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6858329.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="KB950_002144"
     CDS             complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="KB950_002144"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBB6858330.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8598..9347
                     /locus_tag="KB950_002145"
     CDS             8598..9347
                     /locus_tag="KB950_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6858331.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         2811 a         2001 c         1640 g         3003 t
ORIGIN      
        1 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
       61 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
      121 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
      181 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
      241 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg
      301 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
      361 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
      421 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
      481 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgga cgatttgctt
      541 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
      601 taaaaatgta tcgatttctg gcagttcgtg ttctaatatg tctcgaatct tttcagctgc
      661 tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa
      721 ctgatctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
      781 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
      841 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
      901 tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat aaaagtaaac
      961 cgtatcttga acagatagtc aactgcaaac agaaaaaacc gagaaaatca gccagatttt
     1021 ctcggaatat agtctattat tattggtttt acctattaaa tcgaggattc tttcccaaag
     1081 taacgttgcc atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg
     1141 cagtatttgt ccacgaatgt cacaatatat ccttctttat acttaggcgg tgcgatcgtt
     1201 gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt
     1261 tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc gtcaaatttc
     1321 gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta
     1381 gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc
     1441 tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt
     1501 tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata
     1561 attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca
     1621 ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta
     1681 atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg
     1741 catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta
     1801 ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta
     1861 atgcatgtgc ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta
     1921 cagggattac atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca
     1981 tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag
     2041 ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat
     2101 tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca
     2161 ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa
     2221 ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc
     2281 tctgtgtcac aaaaagttct tttatttctg cattagacga cgcagcttct tcgatcagat
     2341 ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt
     2401 ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa
     2461 ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     2521 gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag
     2581 gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg
     2641 tgaccggaac ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg
     2701 acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag
     2761 tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtag
     2821 tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2881 ggctttccac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     2941 tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat
     3001 agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt
     3061 caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg tgataaatag
     3121 ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt tgaacaccgt
     3181 cgataaggtt gtttttgagg cgctttgcct caagtattaa agacaggtag taaaaaagct
     3241 gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
     3301 ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta agtatttctt
     3361 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa tatttatatg
     3421 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
     3481 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
     3541 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
     3601 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
     3661 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
     3721 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
     3781 aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa agctgcaaca
     3841 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaatgga
     3901 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
     3961 gccttgtatt ttacggcacg ctacacagaa ggtatccggg aatcatcttt ttacggcatc
     4021 gatctaggct caccaagtat ggtgcttgtg gccatcgcag gggtcgctta tctgcttcaa
     4081 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
     4141 atcgtctctc ctgctatgat cgtcttcatg tcaatcagtg cgccagccgg tgttacactt
     4201 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
     4261 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
     4321 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
     4381 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
     4441 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
     4501 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt atttaagtga
     4561 atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
     4621 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct aatagtgtac
     4681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
     4741 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
     4801 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga
     4861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
     4921 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
     4981 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
     5041 tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac ggatcgccaa
     5101 tagttgcatc ataagaaaga atctctaaaa agaaaggtat gccttctgct tcgcattctg
     5161 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatcaacgt
     5221 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
     5281 aaatattcaa taaatcaggt aatcttcccg gtattttcgt atcatagccg gttttttcat
     5341 aagctagcag taagccacat tctttctctc gaagcgctgc tgcaggcagg ccgtattctg
     5401 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
     5461 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
     5521 gctggtcgat tgctaatgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
     5581 cctgtttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
     5641 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
     5701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
     5761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
     5821 gttcagtgtg gcgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
     5881 agcaaaacct gacattattc ctgtagtccc ttctttttct ctttctagaa gtttttgata
     5941 tacttctttt tcgtcaagag cgatccctaa acagaatgtt tgctttgcta aaaacttaaa
     6001 tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat gaatgattgt
     6061 catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc tttattatcc
     6121 atcttcttgt ctttatcagt tactcttttc ttttggatta atcctagtat cactccacca
     6181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
     6241 actaaaaaac caccatgtgg tgctggaact tggacgccaa acaaataagt taagacagca
     6301 gcaattgatg agcctaacat catcgtgggt aacactcgga tcgggtcttt cgcagcaaac
     6361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
     6421 cgatcattgg tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
     6481 ggtgcaatac aagctgctga cacaccagcc ataaaagtcg tgtttccttg tgccaaaaga
     6541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
     6601 cagccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
     6661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
     6721 aatgctacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
     6781 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
     6841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
     6901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
     6961 attcccattg ctgcatcgcc aactgctttt accattgccg ctgtagcatt gtattgtgca
     7021 tgatctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
     7081 ccagctacca cgaaaggcaa catatgagag acaccgttca ttaaattttt atagatgcta
     7141 cgaccaaaat tagcaccact catcgcttct ggttctaaag tatcagcagc ttcattatct
     7201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
     7261 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
     7321 ttatctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattt
     7381 tcgatcccaa tttgtccatg ggtctccact ttgatcttga tgcctttttt cctagctgct
     7441 tgttctaatg cctcttgcgc catgtaagta tgcgcaattc ctgtcggaca accggttgct
     7501 gcaattagtt gatattttcc catgtttcaa acctctcttc cttgattttg atttgtttac
     7561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcggtt gagctgcctg
     7621 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatatat cctgctaaaa
     7681 aagcgcctaa catggtatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
     7741 ttcctcgaaa caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
     7801 aaacattttc tgcacctaac tctactagtc gttgtccata aacaaagtaa tcttcatctg
     7861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc gttcggtttc agcaaaaacg
     7921 gtcgataggg taaacaatcc atggcttctt gatcactact atcaataatc aaatgaatcc
     7981 ccttttcctg acaaatccga ctgatttcta ttaaaacctt tggagacagc cctcttggta
     8041 aactcccaga aacacaaaga taatcttctg cttgtaatgc tcttatttga tttaaaaaat
     8101 tatggacagc tgtctgcggt attttaggtc cttgattcac taatttgtat tcttctcccg
     8161 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
     8221 tttggatttg tttctgctgt aaatagtctt ctatatactt tcctgtaaac ccagcagaaa
     8281 aacctaatgc cgtacttggg aaatctagca tttttaatac tagtgacaca ttcacccctt
     8341 ttccgtttgc ctgaatgtca tcctcttttg tgcggtttac cataaaagga tgcatctcct
     8401 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
     8461 acgacctcct tgactctatt atgaaatacg cgaaagagaa aatgttttct taatcgtccc
     8521 aacacaaaga aaacctttac atttataaac tcgtcgtgct attttttaac taacttttgg
     8581 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
     8641 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
     8701 caaatgtctc aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaaggattta
     8761 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttcttag
     8821 ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa tgaacaagaa gtggtacgta
     8881 cgatcaatat gttggaaatc ggaaatattg aggatgcaat acaaagaatc aaatttgctg
     8941 accgggtttt tgtctttgca cgaggatttt cagaaatgat tggtcaagag atgctcgtaa
     9001 aacttcaatt aacagggaaa aattgtcaaa tgcatactga tccagaaatc atcaaaagca
     9061 tcagtcggaa actaactaag aaagacagcg ttatttttgt ttctttgaac ggcgagacaa
     9121 aagaactcgt caccgccgca aagaattgct atgatgcaga gattggttcg attctagtta
     9181 cagccaatca ttcctctaca ctgaaagact attgcgagat caatctcgta ggtttcaaat
     9241 cagaaggttc ttatttccct gattatgaag tacggtcacg tttgcctctc cagattatag
     9301 cgagaatcct gcttgacgcc tatgtattgc gtatggggga gaattgaaaa gcagtttatt
     9361 atggttggtt acagtttaaa acatcacatt ttttattctt tatattgtat ggactcacgg
     9421 aaacttttta tgtataaagt tattcacaat ctata
//
LOCUS       DADVXO010000097         9311 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.100, whole genome shotgun sequence.
ACCESSION   DADVXO010000097 DADVXO010000000
VERSION     DADVXO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.100"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(42..710)
                     /locus_tag="KB950_002146"
     CDS             complement(42..710)
                     /locus_tag="KB950_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6858332.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            844..1131
                     /locus_tag="KB950_002147"
     CDS             844..1131
                     /locus_tag="KB950_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858333.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1547..3085
                     /locus_tag="KB950_002148"
     CDS             1547..3085
                     /locus_tag="KB950_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBB6858334.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3082..4047
                     /locus_tag="KB950_002149"
     CDS             3082..4047
                     /locus_tag="KB950_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858335.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4034..5356
                     /locus_tag="KB950_002150"
     CDS             4034..5356
                     /locus_tag="KB950_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6858336.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5419..5523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5597..6340
                     /locus_tag="KB950_002151"
     CDS             5597..6340
                     /locus_tag="KB950_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858337.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6355..7347
                     /locus_tag="KB950_002152"
     CDS             6355..7347
                     /locus_tag="KB950_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6858338.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7359..8096
                     /locus_tag="KB950_002153"
     CDS             7359..8096
                     /locus_tag="KB950_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858339.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8086..8808
                     /locus_tag="KB950_002154"
     CDS             8086..8808
                     /locus_tag="KB950_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBB6858340.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8892..>9311)
                     /locus_tag="KB950_002155"
     CDS             complement(8892..>9311)
                     /locus_tag="KB950_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBB6858341.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3050 a         1604 c         1967 g         2690 t
ORIGIN      
        1 tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca
       61 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
      121 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      181 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      241 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      301 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      361 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      421 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      481 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      541 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      601 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      661 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      721 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      781 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      841 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      901 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      961 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
     1021 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1081 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1141 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1201 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
     1261 tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata
     1321 gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga
     1381 accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt
     1441 atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa
     1501 aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat
     1561 acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat
     1621 ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc
     1681 caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc
     1741 ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt
     1801 gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc
     1861 tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg
     1921 cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc
     1981 cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg
     2041 gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat
     2101 cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa
     2161 acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt
     2221 atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga
     2281 acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt
     2341 tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca
     2401 aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga
     2461 agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt
     2521 gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt
     2581 ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc
     2641 tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata
     2701 taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag
     2761 tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga
     2821 tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc
     2881 ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa
     2941 agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca
     3001 agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg
     3061 attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag
     3121 tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt
     3181 gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta
     3241 tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg
     3301 cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg
     3361 acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga
     3421 gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat
     3481 cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt
     3541 gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca
     3601 agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt
     3661 ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag
     3721 gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac
     3781 tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta
     3841 gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt
     3901 gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta
     3961 gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga
     4021 gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt
     4081 aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct
     4141 atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt
     4201 agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc
     4261 caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg
     4321 aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga
     4381 tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac
     4441 cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga
     4501 ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc
     4561 ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa
     4621 tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga
     4681 agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac
     4741 tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac
     4801 tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa
     4861 acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg
     4921 agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta
     4981 tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac
     5041 gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc
     5101 gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga
     5161 accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt
     5221 gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt
     5281 gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc
     5341 ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc
     5401 tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt
     5461 gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac
     5521 gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa
     5581 gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt
     5641 aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc
     5701 tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac
     5761 gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc
     5821 cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc
     5881 acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat
     5941 gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc
     6001 ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga
     6061 tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg
     6121 tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc
     6181 tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt
     6241 ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt
     6301 attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa
     6361 aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa
     6421 acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta
     6481 ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct
     6541 ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt
     6601 gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat
     6661 ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc
     6721 gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca
     6781 ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg
     6841 gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa
     6901 tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt
     6961 attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg
     7021 gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg
     7081 gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg
     7141 ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg
     7201 tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc
     7261 aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa
     7321 ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact
     7381 tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag
     7441 atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt
     7501 taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg
     7561 gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg
     7621 tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag
     7681 taagggatta tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat
     7741 tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt
     7801 acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag
     7861 actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata
     7921 tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg
     7981 tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat
     8041 cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct
     8101 cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg
     8161 acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt
     8221 aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa
     8281 caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt
     8341 tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca
     8401 gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat
     8461 tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa
     8521 caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca
     8581 gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga aacggatgga
     8641 ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg
     8701 cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa
     8761 aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg
     8821 acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta
     8881 ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa
     8941 ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca
     9001 ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg
     9061 taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga
     9121 gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt
     9181 ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga
     9241 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
     9301 tgtcctgagt a
//
LOCUS       DADVXO010000098         9290 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.101, whole genome shotgun sequence.
ACCESSION   DADVXO010000098 DADVXO010000000
VERSION     DADVXO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.101"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            12..659
                     /locus_tag="KB950_002156"
     CDS             12..659
                     /locus_tag="KB950_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBB6858342.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            743..1687
                     /locus_tag="KB950_002157"
     CDS             743..1687
                     /locus_tag="KB950_002157"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBB6858343.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1822..3273
                     /locus_tag="KB950_002158"
     CDS             1822..3273
                     /locus_tag="KB950_002158"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBB6858344.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3294..4130
                     /locus_tag="KB950_002159"
     CDS             3294..4130
                     /locus_tag="KB950_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBB6858345.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4130..4789
                     /locus_tag="KB950_002160"
     CDS             4130..4789
                     /locus_tag="KB950_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6858346.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4915..5604
                     /locus_tag="KB950_002161"
     CDS             4915..5604
                     /locus_tag="KB950_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858347.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5610..6566
                     /locus_tag="KB950_002162"
     CDS             5610..6566
                     /locus_tag="KB950_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBB6858348.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6610..7122
                     /locus_tag="KB950_002163"
     CDS             6610..7122
                     /locus_tag="KB950_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6858349.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7378..7926)
                     /locus_tag="KB950_002164"
     CDS             complement(7378..7926)
                     /locus_tag="KB950_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6858350.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8015..9196)
                     /locus_tag="KB950_002165"
     CDS             complement(8015..9196)
                     /locus_tag="KB950_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBB6858351.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         3075 a         1703 c         1873 g         2639 t
ORIGIN      
        1 ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag
       61 ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac
      121 agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga
      181 caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga
      241 tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg
      301 attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa
      361 gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg
      421 aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg
      481 aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt
      541 tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta
      601 catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag
      661 aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa
      721 cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc
      781 caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact
      841 ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg
      901 aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc
      961 aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa
     1021 ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat
     1081 gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc
     1141 caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa
     1201 agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt
     1261 tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc
     1321 tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa
     1381 accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa
     1441 tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat
     1501 tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg
     1561 taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca
     1621 gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat
     1681 ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg
     1741 tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac
     1801 agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa
     1861 aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat
     1921 catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg
     1981 attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt
     2041 caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct
     2101 tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa
     2161 accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca
     2221 agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca
     2281 gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata
     2341 cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt
     2401 ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg
     2461 agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa
     2521 gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt
     2581 gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct
     2641 gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt
     2701 cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc
     2761 tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg
     2821 actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta
     2881 tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca
     2941 ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga
     3001 gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc
     3061 atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa
     3121 ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt
     3181 atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat
     3241 catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga
     3301 aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag
     3361 ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag
     3421 tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg
     3481 gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta
     3541 aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg
     3601 cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca
     3661 aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg
     3721 aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg
     3781 aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg
     3841 atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg
     3901 tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg
     3961 aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat
     4021 taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg
     4081 atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc
     4141 tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata
     4201 cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt tgcttttgac
     4261 agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt
     4321 ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt taccttatca
     4381 attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg
     4441 tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc
     4501 ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat
     4561 ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc
     4621 aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga
     4681 ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga
     4741 aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa aaatagaaat
     4801 ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt
     4861 ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag
     4921 accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc
     4981 ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc
     5041 ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca
     5101 gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca
     5161 agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat
     5221 cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa
     5281 gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct
     5341 ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaca
     5401 gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa
     5461 cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa
     5521 gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg
     5581 ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat
     5641 ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac
     5701 cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa
     5761 aaaagcacca tcgaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg
     5821 atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct
     5881 taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg
     5941 gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat
     6001 ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc
     6061 gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt
     6121 ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga
     6181 tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa
     6241 cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact
     6301 ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca
     6361 tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc
     6421 agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg
     6481 aacttattag tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc
     6541 aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga
     6601 ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc
     6661 attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag
     6721 aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata
     6781 gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc cgtttacaca
     6841 aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct
     6901 ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg
     6961 attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg
     7021 atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt
     7081 gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc
     7141 aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa
     7201 cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg
     7261 tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc
     7321 gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc gatacattta
     7381 tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac
     7441 gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac
     7501 cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa
     7561 tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa
     7621 atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt
     7681 aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc
     7741 atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc
     7801 tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa
     7861 aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata
     7921 attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc
     7981 aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca gtcacaatag
     8041 cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa
     8101 gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga
     8161 agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga
     8221 aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca
     8281 tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg
     8341 caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat
     8401 tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat
     8461 ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt
     8521 caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa
     8581 tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg
     8641 ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga
     8701 acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt
     8761 gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt
     8821 tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa
     8881 taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac
     8941 ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa
     9001 gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata
     9061 atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg
     9121 cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac
     9181 ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg
     9241 caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
//
LOCUS       DADVXO010000099         9277 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.102, whole genome shotgun sequence.
ACCESSION   DADVXO010000099 DADVXO010000000
VERSION     DADVXO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.102"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            104..1939
                     /gene="tdc"
                     /locus_tag="KB950_002166"
     CDS             104..1939
                     /gene="tdc"
                     /locus_tag="KB950_002166"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBB6858352.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2244..3722
                     /locus_tag="KB950_002167"
     CDS             2244..3722
                     /locus_tag="KB950_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6858353.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3688..5019
                     /locus_tag="KB950_002168"
     CDS             3688..5019
                     /locus_tag="KB950_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6858354.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5133..7685
                     /locus_tag="KB950_002169"
     CDS             5133..7685
                     /locus_tag="KB950_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBB6858355.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7692..9131)
                     /locus_tag="KB950_002170"
     CDS             complement(7692..9131)
                     /locus_tag="KB950_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858356.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         2913 a         1555 c         1986 g         2823 t
ORIGIN      
        1 tataaaaaaa aaattctcat ttttggcgct atagtgaaga ggtgttacag ataaacacat
       61 gtatctgcaa gacattgacc aacaaaatat ggagggaaga aaaatgaaag atatggatat
      121 caaggccgtc tttataggcg acaaagctga aaatggacca gtttataaaa tgttgttgaa
      181 caagatggta gatgaacatc ttggatggag agaaaattat attccttcag atatgcctgc
      241 aattagcgaa ggagataagc tcactccaga ttatctagcc acccgcgacc atatgataga
      301 ggtcttggat gaagtcagtc agcgattacg cgcaggatcg atcccatggc attcagcagg
      361 ccgttactgg ggacaaatga atgctgaaac attgatgcct gctttattag cttacaacta
      421 tgcgatgctg tggaacccaa ataatgttgc attggaatct tctatggcaa cttcgcaaat
      481 ggaagcagaa gtaggacagg attttgcttc attattcaat atgacagacg gatggggaca
      541 cattgcagca gatggttcta ttgctaatct tgaaggatta tggtacgccc gttgcatcaa
      601 atcgattcct ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa tgtctgagtg
      661 ggaacttttg aatttatctg ttgaagaaat tttggaaatg accgaaagct ttacggacga
      721 agaaatggat gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc aaagattagg
      781 gaaatggctg gtccctcaaa caaaacacta ttcttggatg aaggcgttag atatttgcgg
      841 agtcggctta gatcagatgg ttgctatccc agttcaagag gattatcgaa tggatattaa
      901 tgctttggaa aaaacaattc gtgaattagc tggacaaaaa atcccaattc taggtgtggt
      961 tgctgtcgta ggaacgacag aagaaggtca agtagacagt gtagataaaa tcgtccaatt
     1021 aagagaaagg ttaaaagatg aagggatcta tttctattta catgttgatg ccgcatatgg
     1081 cggctatgcg cgttcactgt ttctaaacga agcaggagaa tttgtgccgt atgcttcctt
     1141 agctgaattc tttgaagaac atcatgtttt ccaccactgc gtaacgattg acaaagaggt
     1201 atatgaaggg ttcagagcta tttcagaagc ggattctgtc acgatagatc ctcataagat
     1261 gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa cataaaaata tgagaaatat
     1321 catttcttat ttcgcgccat atgtgtttga aaaatcagta aaggctccag atatgttagg
     1381 agcttatatt ttggaaggat caaaagctgg agcaaccgcc gccgcagtct ggacagcaca
     1441 tcgtgtattg ccattgaatg tgaccggata tggtcaattg attggggctt caatcgaggc
     1501 agctcaaaga ttcagagaat ttctcgatca tctgactttt actgttaaag gaaaaacaat
     1561 cgaagtctat ccattgaatc atcctgattt taatatggtc aattgggtat tcaaagaaca
     1621 aggctgtaca gatttgaacg ctatcaatga attaaatgaa aaaatgttcg atcggtcttc
     1681 ttatatggat ggcgatgttt atggtgaacg gtttattact tctcatacta catttacaca
     1741 agaagattac ggtgattctc cgattcgttt tgttgaaaga atgggtctaa cgaaagaaga
     1801 atggaaaaaa gaacagaaaa tcactctgct acgtgcggct atcatgactc cttatttgaa
     1861 tgatgatcga attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg aaaagaaact
     1921 aaacgaaatc atccaataga actgattttt gtgtgaaaga ataacaagag acatatggag
     1981 aacagacaat tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt catatattcc
     2041 tcgccaagat attctttttt ataaaacgtt ctataagttg gatcgttcgt ttcatgtatc
     2101 aaacccagtt cactaagatc gttcaatgga tgtttcggtg atactgggtt ttgtactgaa
     2161 tcgatgaaac cttgtcattt ctgttcttga acaaaaataa aagaaaacaa aaacatatgt
     2221 cgctattgaa agaaggaata aagatgaaag atactggttc atcaaagcaa atcacgacca
     2281 tgacctttat cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt ccagatgtag
     2341 cagcaacgag ctggacgatg attttttaca tgctggttgc agtattattg tacgcttttc
     2401 caatctcatt gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt ggtccagaac
     2461 tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg ctgttatggg
     2521 tccaaatgtt tcctgggatg gtgatggtcg cttcagcact cgctccgttg tttggtaata
     2581 ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac attagttgtc attttagtcg
     2641 tttattggat catcactttt ttgaatttga aatttgatat ggcaaaaatc ggcggaaaag
     2701 tcggtgtttg gttaggattg tacgtgcctt tgacgatgat gctcttatta gggtttgctg
     2761 cgtggatcaa agttgggatc gtaccaaccg gaacattagg cagtttttca tgggacaaat
     2821 taataccaga tactacgact gcatcctcat ttgtttattt tgcgccgatc atgtttattt
     2881 ttactggtat cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca gttaaaacgt
     2941 atattcgcgg agtatttgcc gcgttaattt ttatattttt cgtcaatatt ttgaatgctt
     3001 tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt gaataatatc gcgcaatcta
     3061 tttcgattta ttgccaaatt cttggtttgc cacatcttat tgtgaatctg tttagtttac
     3121 ttgtcttcat tggtgtagca gtccaattat ctgcttgggc ctcaggacca gcaaagacag
     3181 tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg aagacgaatc
     3241 aatttgacgt atccaaatcc gttattttga ctcagtctgt cattatctca atctttgcac
     3301 ttttttattt gctgattcct ggcgtcaatc aagcattttt gatgctcgta aattcaacta
     3361 ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc agttttgaga ttgcgttata
     3421 cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag agcaatcttg
     3481 gagtttggac tgtcgttatt gttttatttg cggcaatcgg attttctgtc ggactgacga
     3541 tgaaagctgg aacatggatc aatctaattg cagtcacagc catcagtgtc attttgtttg
     3601 tagtaccact ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa gtcgccatgc
     3661 tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg gactctaact
     3721 aatcgaaata tccagttaat cgcattaggt ggcgcaattg gaacaggatt atttttaggt
     3781 tcagcaaagg caattgcatc agcagggcca gctgttattc tcgtttattt gatatgcggt
     3841 ttggctattt atggtttgat gcgggccatg ggtgatttat ttttagcaaa tagtacattt
     3901 aactctatca gtgatttcgt tgactattat ttaggcaaaa agtgggcatt ttttgtcaat
     3961 tggacttatt ggctttgctg gcttggagct ggacttgcgg aattaacagc aataggattg
     4021 tatatccatt tctggtttgt acatgtacca gctttgataa gcggaggtag cgcactaatg
     4081 atcttaatgg cagtcaacct tgttgcagtc aagtggtttg gtgaattgga atcttttttc
     4141 tcaatcatca agatcttggg gattttcctt tttatcttat tgggtatagg attgagtgtc
     4201 tcagattttc atacccagtc tatcggaatc aaagaaaatt ttggacagat cgatgaatta
     4261 ctaccgtttg gctttgctgg aatggtagtc tcatttccta tggttttatt cagttttgct
     4321 ggaattgaaa tgatcggctt aaccgttggc gagacaagtg atccgcaaaa gaatttgaaa
     4381 aaagcaatca actgtctgcc ttggcgtatt ctctttttct atataggaac gattttgtcg
     4441 attttatttg ttgttccatg gcattgttta gatttaaaag aaagtccttt agtgactatg
     4501 ctgcaagtga tccattgcca aggcggagca gcaattgtca atctaatgat tttggttgct
     4561 tctttgtcct cagcaaatag tgcagtctac agtactagca ggattttata tcaaacagca
     4621 aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta agaaaggcgt acctttacac
     4681 ggtattttaa ttacaggaat gttatttttt gccggtcttt gtctacattt ttttgtagct
     4741 gatagtcgat tgttttttca gcttttggca ggcatgacaa cgagttgttt tctatttgtg
     4801 tggtcttcga tcttgtgtgc acatgttcaa tttattaaag aaaagaaacg gaacactaca
     4861 caaagatata aagatcttgc tttgttggtg ttttttactt caattgcttt cagcttgtta
     4921 ttcgaaagca cgacacgctt tattcctttt ttattggtta tctggtttgt attcttaaat
     4981 tttgtatata aaagaataag caatagaaat atcagttagg cccagataca ttttgcaaaa
     5041 cgaattattt cagacccccc tgaaataatc gatacgcctg aaaaaacttc cctgcgtata
     5101 ataacagtat caattaaaca ggaggggata ggatgaactg gtacagattg acggtagaag
     5161 atgtattgaa aaactatcaa acatcaacag aaggcttgtc gcaagaagaa cgacagaaac
     5221 gcctgactga tcaaggatat aatgaaataa aagcaaggca gcagacaaaa cgatggaaaa
     5281 aaatagcaaa gcattttact gatctgctga tgatcgtgtt gatcattgcc tcttttttga
     5341 aatttgcttc tagtgaatat attgaaggaa gtattatttt attcgtagtg atcgtcaatg
     5401 gactcgttag ttactggcaa gaaagaaaag cggaagaatc attgaatggt ttgaagcagc
     5461 tgatgggaca agaagcgata gtactatcaa acgggatgca agaaaaaatc cctgcgaaag
     5521 aactggtaaa aggagatgtc attgtcttac gtgccggaga tgtcgttcca gcagatatcc
     5581 gattaatcga agcacatgat ttattagtgg aagaatcgat tttgacggga gaatcagaag
     5641 catctgaaaa aagtcatgcc gttttgacag aagaagaatc gatcggcgac cagaaaaata
     5701 tgggtttttc aggaacgctt gtacaatctg gttctgcagt cggtgtcgtt gtagaaacag
     5761 gaaacacgac agaaatcggt aaaatcaatc aagcattgca atcggtcgaa caacaaacga
     5821 cgcctctgat taaaaaaatc aaccgattaa acaaacaaat ttttcaaggc attctttgtt
     5881 tgagcctatt cttagttatt tttacgacat ttcgatatgg tatggattgg catatccttc
     5941 tttcggctac gattgctttg gtggtgtcaa tggtacccga aggattgcca gctgttttga
     6001 ccatgattct atccgttgga gtacatgaaa tggcaaaaga aaaggcgatc atcaaaggac
     6061 ttccttcggt agaaacatta ggttctatga cagtgatctg ttcagataag acaggaacat
     6121 tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa agaagaagcc tgcgtgttgt
     6181 ccattatgaa aaactgtcag gaattaaaaa caaaagaaga acagaaaaca gaaaatttaa
     6241 gtggcaatcc gacagaagtt gcgctgattc gacacacaga aacagccaat ctacctttga
     6301 aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa atatatggca acgatgcatg
     6361 cggaggaaaa aggagcggtg atttacgtca aaggtgcacc agaagtatta tttgctaaat
     6421 caactctttc ttctgcagaa caagaagcat ggagtcagac agctgcagaa tttgcgagaa
     6481 aaggtcagcg tgttttagga tttgcttata aaaaagtaga ttcgaaacag gaactcacgc
     6541 atgagacttt aacccaatta acttttgcag ggatcgccgg attgatcgat ccgccaaaag
     6601 aaagtgcagt caaagctgta aaggaatgcc aacaagcagg gatctcagtg aaaatgatta
     6661 caggggatca caaagacaca gcaaaagcga ttgcggaaca aattggctta aaacatactg
     6721 ccaaagtttt agaagggatc gatttggatc ttatgtctga tgaagagttg attcagcaag
     6781 tgccaatagt cgatgtcttt gctcgaacga caccagaaca taaactgcga attgttaaag
     6841 ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga cggcgtaaat gatgcacctg
     6901 ctttgaaaag atccgatgta ggaattgcta tgggaatcaa aggtagtgaa gtaagcaaac
     6961 aggcagctga tatggtttta ggagatgata atttccatac gattgcaaaa gccgtaaaag
     7021 agggccggcg gattttggat aatttgcaaa aaaccatcaa tttcttcttg ccaactgcat
     7081 tagctcaagg attgatcttg atttgggcac ttatgctgaa tcgcccgcta cctttgtccc
     7141 cagtacagat tttatgggtc aatatggtaa cgacaatcac tttatcttat gcactagggt
     7201 tcgagtcgcc agatccagaa atcatgaagc gcccaccaag agatccaaag caaggaatcc
     7261 tatctggtta tcatttgttt cggatcatct atgtctccct actgatcatg gttcctgcct
     7321 atttattggc tatgcagttt gaagggcaaa gcttacagca aaccattctt ctgcaaaata
     7381 tcgtcttagc tcaagctgtt tacatgatca attgcagaga gttgtccaag ccgtcattga
     7441 accaaggtct tttgcaaaac aaagcgcttt ttctttcatt aggtatcctt gctttattgc
     7501 aagggatggt tatttttcta ccagtcggcc agcagctgat cggtacagta agtttaacga
     7561 tgacacaaca gttattgatt ggagcaaatg tgattcttct attcttagtt gtagaaatag
     7621 aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa aacagctaga gaatacagtc
     7681 gctagccgtt actataaaaa agttttgaaa gtgagttcag tcaaacgatt gagctcattt
     7741 ttcgttggaa tagaagaaat gtatacaata gggatatcta gagaatctag agggaaattg
     7801 gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa gatcggttag ttcgataggt
     7861 aagaagcaga cacgttttcc taagtaatct tttaattcct gctgcaagaa cgccttccat
     7921 gaaggttctc cttggaaaat aaaatagaca ttgaacaaat tcggtttgat agaaaaaatc
     7981 gtttgttgga tcagtagaga caagagttgg agaaagtaat ttggttcatt gatttctatt
     8041 tcttttttta gatcgttcag taaattctct ttctgttgat acaagtgtgc atattgctga
     8101 tcatatgttt ctaaaaaacg atggtattga cggtccaatg cttcaatcaa tatcggcgat
     8161 tcatgatatt ttaatatgag gagagccaag ttatcagtca gagatgtttt ttttagttga
     8221 tcaagagaca gctctgaagc tagttcctct acaaacgttt cgaggacctc taaaaatgga
     8281 gaatgatgca ggcagcgagc ggtttcttgt ccatagttat tgttgtaatt ccaactttcc
     8341 agaaagcaga aagaagcaaa agaaagctca gtgtctttaa gataaatacc ttccgttaaa
     8401 tacaattttt tcaatgcaga gtcatacagg gaggagagtg tgctgttggt atggatagaa
     8461 gaatctaatc ggcaattcgc ttttatacgg atcgtattga taaagaacca acatgttccg
     8521 aaaatctctt ctgtttcaac cgctagttca gtttgactga ggtttcttaa aaattgaaaa
     8581 taatgggatt catggatcga ataatcctcg aaaaaatagt tgttatgagt gaaaggaatc
     8641 agcaatcgat gataaaaagt acgaatcgca cgctcttttc caagaataca agctgtatat
     8701 ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct gattgatttt agtcacatgt
     8761 cttttcaatg tcccataaga aagcccatgg tcggctagaa aatcagggac agcaactttt
     8821 tttgtaaaaa gcagattttt cagcagttcg acactgactg attgtttgaa aaaaatctcc
     8881 caaaggtcat ttgttaataa attttgctgg ttttgcaaac tgattcctgc ttgatcgctg
     8941 atgatgtccc agccttcggg cagctgagag cgaatagatt gaaggtcaga aaaaatcgtt
     9001 cgttcggttg tccctatgat ttcagctaat tcatttacgg ttatctttgt atgattcagc
     9061 aattgttcta ctaaagaaac atgacgtcga atatcttttt cggtaatgat ttttttcatt
     9121 agtgaataca tcaatcagac tcccttctat tctaagaata cggagaaaat aaacggtggt
     9181 caagtttcaa ctgatttttc aatcattgtt tatttttctg gagcatttac tgatgataag
     9241 aatttcatca tgaacttgaa aaatgatacc actaaaa
//
LOCUS       DADVXO010000100         8919 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.103, whole genome shotgun sequence.
ACCESSION   DADVXO010000100 DADVXO010000000
VERSION     DADVXO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.103"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            23..247
                     /gene="nrdH"
                     /locus_tag="KB950_002171"
     CDS             23..247
                     /gene="nrdH"
                     /locus_tag="KB950_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBB6858357.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            406..2565
                     /gene="nrdE"
                     /locus_tag="KB950_002172"
     CDS             406..2565
                     /gene="nrdE"
                     /locus_tag="KB950_002172"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBB6858358.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2615..3580
                     /gene="nrdF"
                     /locus_tag="KB950_002173"
     CDS             2615..3580
                     /gene="nrdF"
                     /locus_tag="KB950_002173"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBB6858359.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3669..4808)
                     /locus_tag="KB950_002174"
     CDS             complement(3669..4808)
                     /locus_tag="KB950_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6858360.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5117..5830)
                     /locus_tag="KB950_002175"
     CDS             complement(5117..5830)
                     /locus_tag="KB950_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBB6858361.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6084..6785)
                     /gene="nagB"
                     /locus_tag="KB950_002176"
     CDS             complement(6084..6785)
                     /gene="nagB"
                     /locus_tag="KB950_002176"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBB6858362.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7019..8551
                     /locus_tag="KB950_002177"
     CDS             7019..8551
                     /locus_tag="KB950_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6858363.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8742..>8919)
                     /locus_tag="KB950_002178"
     CDS             complement(8742..>8919)
                     /locus_tag="KB950_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6858364.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         2975 a         1742 c         1574 g         2628 t
ORIGIN      
        1 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
       61 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
      121 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
      181 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
      241 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
      301 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
      361 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
      421 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
      481 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
      541 aatacgatga cttttccatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
      601 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
      661 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
      721 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
      781 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
      841 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
      901 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
      961 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     1021 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     1081 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     1141 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     1201 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     1261 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     1321 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     1381 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
     1441 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
     1501 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
     1561 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaaca
     1621 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
     1681 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
     1741 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
     1801 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
     1861 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
     1921 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
     1981 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
     2041 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
     2101 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
     2161 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
     2221 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
     2281 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaacggca
     2341 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
     2401 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
     2461 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
     2521 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
     2581 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
     2641 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
     2701 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
     2761 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
     2821 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
     2881 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
     2941 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
     3001 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
     3061 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
     3121 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
     3181 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
     3241 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
     3301 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
     3361 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
     3421 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
     3481 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
     3541 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
     3601 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
     3661 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
     3721 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
     3781 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
     3841 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
     3901 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
     3961 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
     4021 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
     4081 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
     4141 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
     4201 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
     4261 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
     4321 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
     4381 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
     4441 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
     4501 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
     4561 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
     4621 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaatt
     4681 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
     4741 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
     4801 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
     4861 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
     4921 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
     4981 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
     5041 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
     5101 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
     5161 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
     5221 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
     5281 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
     5341 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
     5401 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
     5461 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
     5521 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
     5581 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
     5641 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
     5701 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
     5761 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
     5821 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
     5881 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
     5941 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
     6001 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
     6061 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
     6121 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
     6181 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
     6241 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
     6301 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
     6361 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
     6421 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
     6481 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
     6541 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
     6601 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
     6661 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
     6721 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
     6781 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
     6841 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
     6901 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
     6961 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
     7021 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
     7081 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
     7141 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca
     7201 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
     7261 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
     7321 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
     7381 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
     7441 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
     7501 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
     7561 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
     7621 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
     7681 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
     7741 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
     7801 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
     7861 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
     7921 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
     7981 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
     8041 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
     8101 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
     8161 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
     8221 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
     8281 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
     8341 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
     8401 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
     8461 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
     8521 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
     8581 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
     8641 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
     8701 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
     8761 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
     8821 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
     8881 ccagccgcac ctccaataaa gtacataaac ataaataca
//
LOCUS       DADVXO010000101         8865 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.104, whole genome shotgun sequence.
ACCESSION   DADVXO010000101 DADVXO010000000
VERSION     DADVXO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.104"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..297
                     /locus_tag="KB950_002179"
     CDS             <1..297
                     /locus_tag="KB950_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858365.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(394..828)
                     /locus_tag="KB950_002180"
     CDS             complement(394..828)
                     /locus_tag="KB950_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6858366.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1060..1770)
                     /locus_tag="KB950_002181"
     CDS             complement(1060..1770)
                     /locus_tag="KB950_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBB6858367.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1760..2620)
                     /locus_tag="KB950_002182"
     CDS             complement(1760..2620)
                     /locus_tag="KB950_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBB6858368.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2625..3263)
                     /locus_tag="KB950_002183"
     CDS             complement(2625..3263)
                     /locus_tag="KB950_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBB6858369.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3278..3577)
                     /locus_tag="KB950_002184"
     CDS             complement(3278..3577)
                     /locus_tag="KB950_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858370.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3620..5104)
                     /locus_tag="KB950_002185"
     CDS             complement(3620..5104)
                     /locus_tag="KB950_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBB6858371.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5124..5585)
                     /locus_tag="KB950_002186"
     CDS             complement(5124..5585)
                     /locus_tag="KB950_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6858372.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5603..6670)
                     /gene="ulaG"
                     /locus_tag="KB950_002187"
     CDS             complement(5603..6670)
                     /gene="ulaG"
                     /locus_tag="KB950_002187"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBB6858373.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6888..7658
                     /locus_tag="KB950_002188"
     CDS             6888..7658
                     /locus_tag="KB950_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6858374.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7931..8758)
                     /locus_tag="KB950_002189"
     CDS             complement(7931..8758)
                     /locus_tag="KB950_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6858375.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2696 a         1863 c         1386 g         2920 t
ORIGIN      
        1 gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga agaacatttc
       61 atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa tttttttcgt
      121 ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt caaaattaat
      181 aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat tttttatatt
      241 agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat aaaatagctg
      301 agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct aaagaactaa
      361 gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa aaagcacctt
      421 ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt gaacaaatcc
      481 tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt cgatttggac
      541 tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc ctattccttg
      601 gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg caaaatcagt
      661 gatccaacga acgattccga taatcttccc ttccccatta cgctttatca gagattcaga
      721 ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc aaccaacgct
      781 cttatataat tcagaaatct catcgatcat aagggaatca atattcattt ttttcttcct
      841 ttcttataca attctccctc taataataca gttctttgac tatttaatct atcacatata
      901 actctcctgt aaagattctc tcgattcctt aaaattcctc tttcctttta gaatgcatac
      961 tttttgataa ataaaaaggt tgtgataaat tttcttcaca acctctgttt ctctctatca
     1021 agtgttatct tttaattttc tacccttacc ctagcatctc tattgcccgt agtatgcata
     1081 ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat tgcgggattt cacatatatt
     1141 ggggttgatc agctcgtttt ctttggccat cagacaaatt tcatctaaga tcaaactgtt
     1201 ttctactgct ttcataggtg aatcccccca tgtgaacggt ccatgtccat agacaagtac
     1261 tccagggact tcgtttggat ctaattttct ttccttaaac gtctcgacaa tcacattccc
     1321 tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt aattggcgtg tacaaggaac
     1381 cttaccataa aaagcatcag catgagtcgt gccataagct ggcaaatcgc gtcctgcttg
     1441 cgcccacatg acggagtgcg tggaatgagt atgagtaata gcagttatat cttcaaaagt
     1501 ttgatacaaa acaacatgtg tggggagatc tgaagatggt tttaagctat cttcttctat
     1561 tatatttccc gacaaatctg ttaccaccat ttgatctgct tgcatgcatt catatctgac
     1621 accgcttggc ttaatgacga tgactcctag ctcacggtcc acttcactca cattgcccca
     1681 agtcaatttg actaatccgt atcgcggcaa tgccagattt gcttcaagga cacgttgctt
     1741 cattgctgca attttttctt cacaagacat catagccagc ctccttcaat ttagaactta
     1801 agtagtcttt tgcctgctgg atttctcttc gaaagtcttg gcttttttca ctccacattt
     1861 caatcagaaa tgttccatca taatctagac gtttcaaatg tcttaataat ccaagaaaat
     1921 caacacaacc gctaccaaaa ggaacatcac gaaactggcc tgaatattct tttgttacag
     1981 ggtaagtatc ttttaagtgg atagcagtga tataatcgat ccctttctca agttctcgag
     2041 ccggattatt ttccggccat gcggacaaat tgcctaggtc tggatatact tgtagaaaag
     2101 gtgaatgaat ctgttctttg atttctaaaa attttgaaat ggagttcata aatggatcat
     2161 ccatgatttc cacggaaaga atgatgccat atttagaagc ttcctttacg ctttctttta
     2221 ttccttcgat aaagtcttct ctggaattca ttgatttctc ttcataataa acatcgtatc
     2281 cagctatttg gattactttg acgcttagat tatgcgccaa atggattgct tttttcatta
     2341 gttgtttcgc tgcttttttc ttttctggat cagcagaacc aaacggaaat cgccgatgcg
     2401 cacttaaaca aatcgaatgg atacgtacac cggagctgaa catctcttct ctcagctcag
     2461 ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc atccgtctca tcaatcgaca
     2521 tctcgataaa atcgaagtcc atttctttta caagggaaaa acgttctttc catgaaatat
     2581 ctttgggtaa cgctttttca taaatcccaa tttgagccat ttccttaccc ccagatacga
     2641 atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat catttgctgc tgtaatacca
     2701 cgaccagtga taaaggtata cacatcgatc ccttcaaata agtgtaatgt gtgtggattt
     2761 aatccaccag ttacagaaac attaaagcct agttcaatca gttttttgat cttcgataaa
     2821 tctttttcac cccaagtttc accagataac agcgcatcac ggctttgatg gtagattacc
     2881 tgccggatac cgatgttatg ccaatccatg gcttgttcaa aagtccaatt gccgtagagt
     2941 tctacttgca attcacctat ttctttttgt gctgcctcca tagttggaat cgttgcacaa
     3001 caaatgactg tcatccagtc tgctcctgct tttgcaacat tactcgccac tgttcctcca
     3061 gcgtctgcac atttcgtatc agcgactagt tttttatctg ggaacatact gcgaatacag
     3121 cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc ccacttcaat aatatctact
     3181 acatcaccaa ctttcataac atctcctaat gcatgttcta agtcattgtg atctaacgca
     3241 atttgcaaat tcggcctgct catcttagtt ccttccttta cttgtttttt tggtaccact
     3301 ttgtttcagc aaatttttca ctgatttctt tgactgacat cacatttttg ataccgataa
     3361 ctgtaactcc ttttttctct gcctgagtaa acatattcaa aaaattgact ggcgtaaaaa
     3421 ctacatcgta ttggccagct gaactttttc cctctgaaat ggcacaatga tggattttgg
     3481 taattggtac gtttagttct ttcattgctt tttccacact tctcatcatc atcaagcttg
     3541 ttcctgatcc atttgcacac gatactaaaa ttctcatctt aatccctcca ttatgagtgt
     3601 tgtctaatca gattttcttt tatactctct gcttttctgc atatgtgtca tagtcttcta
     3661 caatcaaaaa ataaccatca ggatttttcc gatattgtaa ctgcggaata gccaataaac
     3721 caatgacaac tacggcaatt ccagcataac ctaggacttt catgattact gtaaacaatg
     3781 gccaagctgt tgcccagtcg aacatcccga tataaccacc gtatgttgac atcccaatcc
     3841 atgtagcgaa taatgctgaa ccgcctactt gtatgattcc tgaaagaaac gggaacaggc
     3901 aagcggcttt gaatccacct cggttattgg cataaactgc tataacggca ttatcaaaga
     3961 acaacggaat gaaaccggct atgacgatcg ttggtgattt caataaaatc agtaaaccaa
     4021 tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat cgttaccgca tttggcgaac
     4081 caaagccaaa agtagcagct acgtcaattc ctggaacagc tcctggtaaa atcgtgtttg
     4141 agattccttg gaaagattga gttagttcat cgacgaatgt ccgaacacct aattgcaaga
     4201 tcgccagata taccgcaaaa tttaaagaag tcgtcataat gtagaataag aaactctgcc
     4261 cttcttgcat gaattgcgct tgaatcaaat aatctttgcc tagtacaatc agaatgatcc
     4321 caaagaaaaa tagcatgagt aacgaagtag caaccatatt ctcattgaaa atcgacaaga
     4381 atcctggtaa tttgatatct tcaatttttt tgtcttgctt gattgatcta tttttctttt
     4441 catcccgctt tttcatccac tcagctaatt ttgcaaatat aaagattccg aacatttgct
     4501 gatgtgccac tgcaaatccg gcaccttctg tcaattcctg cgtgattcct actgttaagt
     4561 tagaagctac tgtccaataa caacctaaaa tcagtcccat aaaaatcaat gcttgtatcc
     4621 tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc ggtagctgct tgttgaacct
     4681 gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata tttctgaaaa cggactaata
     4741 agatatttaa aacaaatgca atcagtaata aaatcatcac atctccaaag gtacggccaa
     4801 atgtctccat taatccagca tccacagcat tttgtccaaa ataaggatct gtaaccatgg
     4861 catccaaatt gaaccgttcc tttagtccaa ccaagattgg ccgaaaatta ttgaccaaac
     4921 caccagagcc aacagtcaaa ataaagtaac cgaccgttgc ctttaagaat ccagcgatgc
     4981 attcatgaag cgggcgtttc agcaaaatat agcctaataa cacgataaaa ccaatcaggt
     5041 atgctggctg cgtcaagata tttgttgcaa aatacgtcca aatgcttaac aaaaattccc
     5101 ccatcttttt tctcctctat caattaatta tattctgcca tcacaacttg gtaatcatcg
     5161 atggagttcg tagagatgag acgctcaacc aatccatctg tcattaataa ttcggataat
     5221 ttttggatat tttctagatg ttcttctgga tcttttgctg cgagtgtaaa aaacaaacta
     5281 gccgttttct cctcattctc cccttcactt tcaaaacgga ttggctgttt caacttggta
     5341 aatgaaatag ctgttccaaa aacaccatca cttttttctg aagaatgcgg cattgctact
     5401 tgaggtgcga tcacaatata aggaccgtat ttttctacac aatggatgat ctcctccaca
     5461 tacgtttcat caataatttc cttatcgatc aatacaagac agctttcggc gatagcttca
     5521 cgccaatccg tgatttcttt atctgggtaa tgaatcaatt gatggtcaaa aaagtatttc
     5581 agcaagaaac tccccttctt tcttataaaa acgacggaaa cggtgtatca ttttcaatcg
     5641 agaagacatc ttcaaatcct cgataaaaat taaactcaag atcatctttg tttgtcggat
     5701 agatgaattt cccgcctact tgccagatat aaggtttaaa tccatacttc aggcgatcct
     5761 ttttgaattt ccaaatttca gtgatctctt ttggatcagc cataaaatta gaccaaatat
     5821 cgtagtggac aggaatcact acttttgcat tcaatgactc cgccatgcgc agcatatcaa
     5881 cagaagtaac tttgtctgtt atccctcgtg ggttttcacc ataggcccct aaacagacat
     5941 cgattttgtg ttcattacca tgtttggcaa ataaattgga gtaatgtgaa tctcctgcat
     6001 gataaagatt ccctcctgat gtttcgaata aatagtttac tgcaatttga tccatctcct
     6061 gaggcatttt tccttttaat acctcttctg gatcttcaca agtgaccaac gctgtccgat
     6121 caaacgcttc taaagctact acattgatgt ctttgatttt gactgcatca cctggcttta
     6181 ctgtaatcgt tttttcttca ggaattcccc atttttgcca cgtgcgaacc acttcttttg
     6241 gcccaatgaa tgttgcttca ggacaatttt gatgaactgc tgcggctgta tggatatcta
     6301 gatgatcgga atggatatgt gtaacgatta aagcgtctac attttttatc gcaaaaggat
     6361 cgattacgaa aggctgagta cgtaaattag gctgcatatt ttgaacgcca ctcatacgca
     6421 tcatctgatg gccttttttc atcatgccat tcccatgact acgttttccc gtcccacacc
     6481 atagatcaca taaaatattc gttccttcat gacttttcag ccaaattccc gtacagccca
     6541 gccaccacat agcaaaagtt ccaggatcca ctacttctgt ttcgatttct tcattcaagt
     6601 atgtgcccca ctcaggaaat gtttctacta accacttttc ttttgttact tcattgacag
     6661 ttttactcat ttgcttgctc cccccatatc gttgttccaa cttacaagta aatattaatg
     6721 tgtatcagca tccatgaaaa cccttttata tgaccgtttg ttttttatat gattttcttt
     6781 cagtaatcaa tgattgtttc agtataataa agacagatat gaatgtttaa atgaacaact
     6841 agtcaaataa atccatggat caagatgtcc ctcaggaggc aaaagtcatg aaaaactcac
     6901 ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact gcttcagcag actgaacaac
     6961 tatcaacttt cgaattagct gaatcccttg atgtatctat tagtacgata cgaagagatt
     7021 taaatttatt agaagaaaaa aacgagatca tacgcaatta cggttattgt tcttataatt
     7081 acaaaaacca gactgatttt gatcaatctg gtcctgaaag gattaaacaa gcgattgcca
     7141 gagaagctag taaatacatt aacgattatg atacgctgtt tatcaattct agctcgactg
     7201 cgttgaaaac actggatttt cttcaatcta accatttgac agtcattaca aacaacgtga
     7261 aaatcaatta ctctccgcac aaagataatt gtagttatat tttaactggt ggagagatgc
     7321 gctttccaaa agaagcacta gtgggcgaca tcgcattaaa tacgatttct tcagtcaatg
     7381 cagacatttg tatcattggc tgtagcggag tcgatgtagt acatggggtc acaacaaaga
     7441 tattaaatga atcaacgatc aacgaaatga tggtcaacaa gacaatcaaa tgtaagattt
     7501 tagtcgctga tcatcgaaaa atcggactga catcaaagtt caagattgct gatatttcag
     7561 tctttgatta tttgatcact gataaatttt gttctgaaaa agtattagag gagatcagaa
     7621 atacggggat aaaaaccatt caagtagatt cattttaaga accaggacgt aaatatgtaa
     7681 ataggattat caaaaaaatg aataacaaat ttatcgataa atgaaaaaca cgctacgctc
     7741 aaattgataa aggaatccca agatgtatcc atccaatcga taccatcata atctattgct
     7801 tcttttataa ccgttatatg ttgcctaaat aggaatcaaa caatatcgaa aaaaggaggt
     7861 atgacaaaag tcacacctcc ttttttcgaa taaacggtat tcaaatcaga acactgattt
     7921 cacaaccact ttatttcagg tcgttttcaa tcacctttac cgcgtaatca tttccgttga
     7981 aacgcgctat tttttttgcg tccatatatc tttctaaata atcatcttct cctaattgct
     8041 tatggcaaga agcaatttca taaagcaagt attccaatgc ataagtcgat ttatgtttct
     8101 tattatactc aatacccttc tcacataatt ggattgcttt atcataatcc tcgattttac
     8161 ggtaataggt ggcgatatca taatatacaa tgcagaccat cagattatca aaatcagtct
     8221 cactcaatat tttaagtgta cgctccacat aaactttcgc aaaatctaat tctccttttg
     8281 acaagtagat cattgccacg tttgcagctg cttgtaagtg atggatatct gtttcggtcg
     8341 tttgttccaa cacttgatta aaataaaata atgctgcatc tggttttttc gcataaagat
     8401 gatcaagcag tccttgataa aataaaattt gatttaaaag atcagaatta cttacttttt
     8461 cctgatcgat cgtttgtaaa attttataag attcttggta ttttgataca gaattcaatg
     8521 attgaatctc atttattttt ttcctgattt tgtcttcttc tgtttctact aatatatcgg
     8581 agatctcgac attcagcttt ttgcaaagcg cacttaagat attgatgcct ggcgacatat
     8641 tcttgttttc gatattgctg acagttgctt gttcacaaat gccttctgct aattgtactt
     8701 gtgttaaccc ctttttcttt cggtattctt ttagtaatgc tccatctact ttaatcaaaa
     8761 caatttcctt ccctttaaaa aattatttct cctttatatc atacacaaaa aaaagttcta
     8821 aacaaccaaa aatgtttaga acagaataaa aattttaatt ttaaa
//
LOCUS       DADVXO010000102         8784 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.105, whole genome shotgun sequence.
ACCESSION   DADVXO010000102 DADVXO010000000
VERSION     DADVXO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.105"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(382..2763)
                     /locus_tag="KB950_002190"
     CDS             complement(382..2763)
                     /locus_tag="KB950_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBB6858376.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2753..3400)
                     /gene="recU"
                     /locus_tag="KB950_002191"
     CDS             complement(2753..3400)
                     /gene="recU"
                     /locus_tag="KB950_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBB6858377.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3464..4003
                     /locus_tag="KB950_002192"
     CDS             3464..4003
                     /locus_tag="KB950_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBB6858378.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4093..4512
                     /gene="gpsB"
                     /locus_tag="KB950_002193"
     CDS             4093..4512
                     /gene="gpsB"
                     /locus_tag="KB950_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBB6858379.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4534..4901
                     /gene="rnpB"
                     /locus_tag="KB950_002194"
     ncRNA           4534..4901
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KB950_002194"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5110..6276
                     /locus_tag="KB950_002195"
     CDS             5110..6276
                     /locus_tag="KB950_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBB6858380.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6322..7818
                     /locus_tag="KB950_002196"
     CDS             6322..7818
                     /locus_tag="KB950_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBB6858381.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7899..8582
                     /locus_tag="KB950_002197"
     CDS             7899..8582
                     /locus_tag="KB950_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6858382.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2774 a         1586 c         1855 g         2569 t
ORIGIN      
        1 gatggtgcag acaaattatg ataattaagc atactatcag ataactcaaa atgaagcctt
       61 ctttcaaaaa attattcaaa accatatatt gtatttttta tttgataata acaatgggat
      121 tattaagaag tggcactaaa taaaagacta gtgtgtattt agccgattta attataaaca
      181 agtaaatgga taaagtatat gtaatgataa aagaaaaatg gattttctaa tttttaagct
      241 aaatgttaga tcacacatgt atatgctgga tagtcttata aaaaatcatg taatacagct
      301 tggtacatga tttttatttg tagctgtttt ttacatagac acaaaaaaga aaccacgctt
      361 gtagacgtgg tttctttttt actatctgtt tccagaagct gaggtgctgc tggatggtgt
      421 atggtcattt gcagcatccg ctccgctagt tgctggctca ctggaagaag caggaggagc
      481 agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc
      541 tggctcgctt gtttctgctg tcgtgctttc ttttgaggac tcagcagttg attcgctttg
      601 actagtagag ctagagcttt gagtcgttgt ctctgttgtt gagcttccag gagtctggac
      661 atagctggaa gatggaatag ttgttgatgg tgtaattgca ttcgatctag ctgtatattg
      721 atctttgtag tacagttcac cgcccacacg aattaatccg cttggcatct cccagtctgt
      781 atttgtcacg ttagctgaaa catactgcat aagctcgcga taaacatcag aggctacgtg
      841 tgacgattct gaagtgacag gcgtcatttt tttattgtaa cctgtccaca cggaaatcga
      901 ataatttggc gtatagcccg caaataggat atctggatac accccagaag aaataccaag
      961 ttttgcatac tcgtcatctg tgtaattgga tgttccggtc ttaccggctt gatataaccc
     1021 agcgatttgc gcattcgtac cagttccttc agtgatcgta tcttttaaaa tgtctgtgat
     1081 catataagct gtttctggag acatggctgt ttttccatct ggctcataag tttcttctgt
     1141 tccgtcttgg aagacgattt tattaacata ttgaggtttg taataagtcc ctccattggc
     1201 gaaagccgca taggctgctg ccatttttaa ggaagaagct ccgtattttg tcccatcttg
     1261 ttcttccgta ttactagaga tcgcattcga ttgatggatc gtactgtatt cgatcccaag
     1321 attttttaag aaagaagcca ctttatctga accgacttca ttgaataatt tgactgctgg
     1381 aacattacgg gataagtaaa gtgcttggcg taaagtaatg gtgcccatat attgattatc
     1441 ccagtttcct acaggagtcg aagtcccttc atagttatac ggtgcgtcag taatagtttt
     1501 tccagtagaa tatttcaaat attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt
     1561 agaaccaaaa tcacgggaag tattcactgc taagttgttg cctaaagtta cgtcttctgc
     1621 gatgtgacgt ccgccaatct gggcttttac tttcccggta ttggtgtcga taagggtgga
     1681 ggcaacttgc atctcgtcgt ctgggtaaga gacatactga tccgtgttga cgatatcata
     1741 tagtttcttt tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac
     1801 attcttatct gttttttctt gtacttcatt gatgacttct ttgacataat tatcgacgat
     1861 cttagtattg tcatcggatt gagttagttc ttgcagacca tccgttaccg gtacattaac
     1921 agcttgatca tattctgttt gagagatttt ttcgttttga agcatcgtat acaaaacagt
     1981 atctcgtctt ttcttcgcct gatctggata aacataagga tcataggcgg aaggggcttg
     2041 aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa
     2101 gtacatctca gaagctgtct ccatcccgta caagccgttt gacatataca ctttatttac
     2161 gtagtaggtc aagatctctt gtttcgattt tttttgttcc aatcttacag ccatccacgc
     2221 ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg gtggagaaga aggaaagttt
     2281 gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa agttagagag
     2341 ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc
     2401 taccgacacg atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg
     2461 cttttctgcg cctaaatctt cgaatagttc gccatcttgt gtatatagtt tggaagatac
     2521 ggtagcgtct aattttttat ccgtcaattc gggcgcatct tttgcgtaat accagaaaag
     2581 tcctacacct gctaaaaaga ggatacagaa aaatgagagt attcctaaaa ggatcttgat
     2641 aaataatcct tttttatgtg ttccagagga ctttccagaa cctttgccag aattcttctt
     2701 cacactcttt ttaggagtag gctgtttctg tcgtctagag cttctagttt gttcatttgc
     2761 cattcttgct ttctccttca atagataatg ttgataaata gaagtcaacc gcgtccaaat
     2821 aaggaatccg cggagaaata ccagttttga tctctattcc tagttcttcg atcaaagata
     2881 aaggtagtga tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc aaatgaacac
     2941 tattcaaata aaaacagcgc ttcaaagacg aaaaccataa caagacaaag cagaccccat
     3001 tttgttcgag gcatgctttc atatgttcga tttgatgggc atggaagttt ttaaagggaa
     3061 aagatgtttt gttcttggtt tcttttgctt caaagtccaa gtaaaagcct cgatagacgc
     3121 cattatagtc tgtagttgag gcttgggtaa agtaggcttc cttgatcaca gcagcacttc
     3181 tcttaggata gtctactttc acgatttgga taggggtagg ctttttatgg ataaccgcca
     3241 acccgcggga aagataatat ttattacttt cattgattgc ttcttcaaag cgcatccccc
     3301 gattgccgaa ttcagtgatc tgatttcttt tttcttgaat tttttcgacg ggagaggcat
     3361 tcttataata aggttggcca tttggatagc gcaataccat ctttaatcac actcctaggc
     3421 cacattatag caaaaaagcg gatagaaaga aaaggaatct gagatggata agtcaaaagt
     3481 aatctatgtg tctgggtatc gaagtttcga actcggtgtt tttaaagaaa atgatcctaa
     3541 aatccaagtt gttaaaaatg ttttaaaaaa agaactccat cagttagcag aagaagggct
     3601 ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc
     3661 ggaattgaaa gaagaatatc cggaactatc tcttggtatt ttgtatcctt tttctgattt
     3721 tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga
     3781 ttatgtcgaa gctgttagcc atcaacccta ccaatcacct agtcagctaa agaatcacac
     3841 tcgttttata cttgagcata cagtgggctg tctgctgatc tatgatgaag aattccctgg
     3901 aaaaacgaag tatttttttg aagatgcaca gaattaccaa gcagatcatc cctacgaaat
     3961 tcggctgatc agtatggacg atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat
     4021 ttatcaactt ttttcctata caatgaaaaa ggtagaaatg ttattacgta aatgaaaact
     4081 agaggtgtaa atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc
     4141 aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc
     4201 aaagattatg aaacttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg
     4261 agtgcaaaag tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa
     4321 acagaggcac caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat
     4381 cttgaacgtg aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca
     4441 caacctaatc caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca
     4501 cgtcaatttt aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg
     4561 atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag
     4621 cgaaaccata agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg
     4681 ctatgcttaa gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg
     4741 tggaacgcgg taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct
     4801 ggaagaagaa caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt
     4861 ttctccctgg aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa
     4921 ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt
     4981 tcaatacttc agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg
     5041 gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg
     5101 agattgtgta tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta
     5161 gaagcactgg ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga
     5221 cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca
     5281 gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt
     5341 gaaaacgtaa aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta
     5401 gctggaaaat cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa
     5461 aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg
     5521 gaaactggcg cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact
     5581 ctggatacga caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca
     5641 ccattgaaag aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt
     5701 ccattttatg atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc
     5761 cataatatcg caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt
     5821 caagaaatca tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa
     5881 ttagatatta ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca
     5941 gaagaaatcg gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa
     6001 acggataaag aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa
     6061 gaagaaactg ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca
     6121 tggagtaaat atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca
     6181 accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga
     6241 actcgtccgc ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt
     6301 ggatgtttgg aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc
     6361 aatcttctac aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa
     6421 aaagctagtg cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa
     6481 aaaatcggtc caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg
     6541 tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc
     6601 gtaccggaag atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg
     6661 caaaaagcta gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt
     6721 gaattaacaa aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt
     6781 ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag
     6841 cttaaagaag gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa
     6901 acagactttt taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta
     6961 atcgaacaac ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt
     7021 atgactggaa ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc
     7081 aaaatggctg tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc
     7141 gatgataaat atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca
     7201 caatcgttgt ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat
     7261 cctttctttc aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat
     7321 cgttcattga accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac
     7381 cctctccata tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat
     7441 gttgctgaac tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca
     7501 acaaatgatg tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat
     7561 tttccctctt atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa
     7621 gaaatagatg tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg
     7681 tcagagcata tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca
     7741 acacatgaac cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat
     7801 gtatatcagg taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag
     7861 atgttttcag ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg
     7921 ctgattatag tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca
     7981 aaagagttgt caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg
     8041 tgacgcttgc agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga
     8101 acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga
     8161 tctcgatgat tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg
     8221 ccgaactatc tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag
     8281 caatcggcat tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc
     8341 ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga
     8401 tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat
     8461 atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg
     8521 aatggtctat tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat
     8581 gatggaggtt gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag
     8641 ttacttctgg aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca
     8701 aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga
     8761 attggcagga gaacaccgtt tatt
//
LOCUS       DADVXO010000103         8750 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.106, whole genome shotgun sequence.
ACCESSION   DADVXO010000103 DADVXO010000000
VERSION     DADVXO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.106"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            45..1106
                     /locus_tag="KB950_002198"
     CDS             45..1106
                     /locus_tag="KB950_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6858383.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1109..2005
                     /gene="murQ"
                     /locus_tag="KB950_002199"
     CDS             1109..2005
                     /gene="murQ"
                     /locus_tag="KB950_002199"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBB6858384.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2019..4001
                     /locus_tag="KB950_002200"
     CDS             2019..4001
                     /locus_tag="KB950_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6858385.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4066..4512)
                     /gene="nrdI"
                     /locus_tag="KB950_002201"
     CDS             complement(4066..4512)
                     /gene="nrdI"
                     /locus_tag="KB950_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBB6858386.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4723..6147
                     /locus_tag="KB950_002202"
     CDS             4723..6147
                     /locus_tag="KB950_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6858387.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6328..7101)
                     /locus_tag="KB950_002203"
     CDS             complement(6328..7101)
                     /locus_tag="KB950_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6858388.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7098..8003)
                     /locus_tag="KB950_002204"
     CDS             complement(7098..8003)
                     /locus_tag="KB950_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6858389.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8157..8477)
                     /locus_tag="KB950_002205"
     CDS             complement(8157..8477)
                     /locus_tag="KB950_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBB6858390.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2746 a         1575 c         1869 g         2560 t
ORIGIN      
        1 tctagtcata gaaatgaaga cggatacaga aaaagaggga taatatgttt ggcttttcaa
       61 tatttatgaa cgattctttg tcagaagaga agaaacagta tatccgccaa atggcatcca
      121 atggctttgt cggtattttc acttccatgc atataccaga agatgatgtt tcggcatatc
      181 aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa taaactagaa ttgatggtcg
      241 atatttcagg agaggcattg gaacgagcgg gtttttcatt agaagatcta acgcctttga
      301 aagcaattgg tgtaacaggt ttaagaatgg attatcatat ttccaatcag cagattgccg
      361 actggtcaca tcaattgaaa atcagtttga atgctagcac gatcacgcct aaagatatcg
      421 atgaattgaa agaagcagaa gctgatttct cacagattga agcttggcat aattattatc
      481 cacgtcccga aacagggctg gacaaagaat ggtatcagaa aaaaaatcaa tggctcaaaa
      541 ttcaaggtct ccttgtccaa ggttttgtac caggagatac agagttgaga ggcccactat
      601 atcaaggatt gccaactttg gaagaacatc gagggatcca tcctcttgca ggcgctctag
      661 atctattagc aagtaacacg gatattgttt acatcggaga tgcaggtctg tcagaaaacg
      721 ttcaggagca atttgcttct ttccaaaaag aacagacagt cttgctccat acggaacctg
      781 tagatgaaga attttacgaa tatatattag ggaagcatac gaatcggcaa gacgatgcaa
      841 gagatgtcat acgaagcgta gatgcacgtt ttcgagagat tccgcctatc ccagcaagaa
      901 atacagctac gcgtatgaaa gggtctatta ctttagataa cgaaaagtat ttgcgctata
      961 tgggtgagat ccaattaacc aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag
     1021 ctaaagttat caaagaagag ctaccattga tcaaccaaat caaagctggt atgaactatc
     1081 aatttataag aaaagaagga agataaaaat gagcaaaatc aatttagaga acctaacaac
     1141 ggaacgcaga aaccaagaaa cttttggatt agatgagatg agtatttctc aagccgttca
     1201 actaatgaac aaagaagatc agaaagtggc cgctgcggtt gaaaaagaac tgacaagtat
     1261 cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa ggtggacgtc tgatttatat
     1321 gggagccgga acaagtggaa gactaggtgt gttagatgct gctgaatgcg taccgacatt
     1381 cggtgtagaa ccggaaatgg tcataggtct aattgctggt ggagaagaag caatgactgt
     1441 agctgtcgaa ggggccgaag attcttatga gctagggcaa gaagatctaa tcaagctgaa
     1501 tttgacaaaa aatgatatag ttattggtat cgcagctagc ggtcgtacac catatgtgat
     1561 cggtggatta gattacgcaa aatcgatcgg tgctgtcaca ggaacgattt cttgcaataa
     1621 acaagccgaa atcagtaaac acgcggattt cccaatcgaa gtcgatgcag gtccagaatt
     1681 tttgacaggc tctactcgat tgaagtcagg aacagcacaa aaacttatct tgaatatgat
     1741 ttctactatc agtatgattg gtataggtaa agtattcaat aacttgatgg tcgatgtcaa
     1801 accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc atcatgcaag cgacagaagc
     1861 tgattacgaa acagctgaaa agtactttga agaagcagat caaaatgtta agctggctat
     1921 cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa gcaaaattag ctgaagcaaa
     1981 cggatttgta aaagggacct tatgaggggg aaaagaccat ggcaaaaaac gaactgactt
     2041 tggaagaact ggcaaaacaa atttataaag gggcaggcgg catgggcaat gtagaaagca
     2101 ttgtccactg catgactcgt gtgcgtatga agatcatcaa tgatgatctt gtaaatgtct
     2161 ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga agatgaacag cttcaagtca
     2221 tcatcggacc tgggaaagtg aacaaagtag cacagcaaat ggttgatttg gcaggcgtga
     2281 aattaggaga aaaactttct tctgtaagta cagctacagc aaattcatca gcatctggaa
     2341 aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac
     2401 catctaaaat caaatcggta ttaaaagaca tttctaatat ctttgtgcca atgattcctg
     2461 catttgtcgg tactgggatc gtggcaggga ttgccgcagt tttagcaaac ttagtcacag
     2521 ccggaacact tgatgcagca acatggcaac aatatatcga tgtgatgaac atcttgaaaa
     2581 acgggatgtt tagctaccta gttatctata caggtatcaa tgctgcacaa gtttttggtg
     2641 caacaccaac tcttggtgga gttatcgggg ccgttgtgat gctgacaggg atgaatccgg
     2701 aagcaccact gaaaaactta tttactggtg aagctcttgc agctggacaa ggtgggatcc
     2761 taggtgtcat cttcgctgtt tggctattgt caatcgttga gaaaaaactg cataaagtag
     2821 tacctgatgc ggtagatatc atcgttacac caactctttc attgattgcc atcggtctga
     2881 ttgaaatttt cttcatcatg cctttagcag gtttcatttc tgatggcatg gtcggtggga
     2941 tcaactgggt acttggcgtt ggtggagcat ttgccggctt cgttctaggc acattattct
     3001 tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc aatccatgtg caaatgattg
     3061 atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat ggctggtgca ggacaagtgg
     3121 gtgcagcatt ggcattatgg attcgctgca aaaaagataa agaattaaca gaaatgatca
     3181 aaggagcttt accagtcggg atcctaggaa tcggcgaacc attgatttac ggagtaacat
     3241 tacctctagg acgcccattt attactgctt gtatcggtgg aggtatcggc ggggcagtga
     3301 tcggaatgat cggaaatgtc ggggctatcg ctattggacc ttcaggagcc gctttgatcc
     3361 cattgatctc tgacggtaaa tggttaggct acatccttgg attattggca gcttatgctg
     3421 gtggcttcgt tgctacattc ttcttcggta ttcctaaaga ccgtttagaa aaagaagaat
     3481 tagcgactga aacagccaca gtaaatgaag tgcctgaaaa agtagtacct caaacttcag
     3541 ctgaaagtgt gactatcctc tcatcagttg ctgacggaac agttgtttct ttagataaag
     3601 caagtgatcc agtttttgca caaaagatga tgggtgaagg ctactttgta gaaccagaaa
     3661 acggacatat ctattctcca gttactgggg tagtcagctc tgtcttccca acgaaacacg
     3721 ctattgggat cacaacgcca aatggtttag aaatcttgct tcatatggga atcaacacag
     3781 ttgacttagg cggtacgcca tttgatgtga aagtaacaga agggcagcaa gtgacacctg
     3841 atacgcttgt agcagaagct gacttagaag cgatcaaagc agcaggcaaa gaaacatcca
     3901 tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa aaaacaggta
     3961 aagcaaaagc aaaagcacct gtgatggatg tcaaagctta atatgataga aacaatagaa
     4021 acgaaagaag aaactgaaat ctatttttca gcttcttctt ttttattatt tattgttttc
     4081 tgctaaaatc tgataaattc gtgtcacatc agatggtgtt ccccgtaatt cataatctgc
     4141 caagaaagga aagccaaatg tttgtgcata ttgtttggct gttagacaat actgattatt
     4201 gaagttttta tttccgctac cgactacacc aagacaaagt ttatgattgt cttcatgttc
     4261 cagatattca cgcatcgttt cggtcaagat ttctgtatca ccgttatcta aaccatttcc
     4321 gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct gtttctttcg taaaatcact
     4381 attttcatgg atttctttta aagtaacttc aggattgaca ggatcttctg catgcatttt
     4441 ttcagcatat tctgctagat gtttggcaaa ggcacgcgta tttcctgaaa tcgatatata
     4501 taaaatgttc atggatcatc ctccaattct ttccttttag tatagcatct tttttaagtt
     4561 ttttggtggt gtaatttgga aagggactag gcgctttgct tgtataatga gaaaaaagcc
     4621 ggtagaatta tatttctatt aaagttttta ttaattaagt aaaaataaaa aaggtttgtt
     4681 atactttttg ataaaagcgg ttttattttg gaggatgctt acatggatac agagcaacag
     4741 aaaaaaggaa ttggtttaat tccacttgct gccttagtaa ttggttcagc gattggaggt
     4801 ggcgtatttg gtattatgac ggacatctcc ggatcggctg gcggcccagc tattttcagt
     4861 tgggtactcg taggaacagc aatgttgatg ctctccttgt cgatcaataa tatcaataaa
     4921 aaacgtccag atttaaaagg cgggatcttt agttacgcag aagaaggttt tggccgtttc
     4981 gctggtttta tcagcggttg gggttactgg ctcacatgct ggttaggcaa cgtagctttt
     5041 gcaacattgt tgatgagcgc attaggatat ttcttcccag tatttgaagg tgggcaaaat
     5101 ctggcatcga tcgtgatgag tacagtcttt ttatggggat atgcatggct agtgaatcgg
     5161 ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat tctgtaaatt agtgccatta
     5221 tttttattta ttgtagtagc tatcagcacg ttttcgatcc aacaattcat tgataacttt
     5281 tctagcgaca ttatattgaa tgcgaatgga cagccgatcc cgttttccag tcaagtattg
     5341 agttgcatca tggtgatgct ttgggtattt gttggtattg aaggagcctc tgtcgtaggt
     5401 tcacgagcaa aaaaacaatc agatgttggg aaagcaacga ttctagggat attcggcttg
     5461 attattattt acgtcttggt ttccatgctt ccatatggga caatgacgat gaatcagctg
     5521 caaacgatca aggcgcagcc tgctatgggc tatgtgtttg aaatgatggt tggtaaatgg
     5581 ggagcagtcg tgatcaacgg cggtttgatc atttctttag ttggtgtttg gctctcatgg
     5641 acgatcttac cgattgaaac aatgggaaac atggctgaag acggactttt gcctaaagca
     5701 tggggaaaac tcaacaaaaa tggggcacca acttatgcta tcgtattgac gacgttatgt
     5761 acgaatgtct ttttactttc attattattc actgacagtg cttataactt tgcttacaca
     5821 cttggtacag cagctatttt cttcacgtgg cttttcttag gattgtacca aatgaaactt
     5881 tcttatcagc ggaaagaatg ggtccaattt ttagtagggc ttttagcaag tgcctttcaa
     5941 gtatgggcta tgctagtcgt agcctttaat gaagtaatgc ttgttcttgt tcttttcttg
     6001 ccaggcattt atttttacct caaagcacgt aaggagcaaa acgagcctgt acaacatatc
     6061 cacttagata agattttact tataggtgtg acgatggtcg gagtgattgc aatggtttta
     6121 tttgccactg ggatgcttca cgtatagaag tataaaagaa gtataaagag aggttgtgag
     6181 cctgtcgttt attcaaaaac atactaggaa aggagctgtg acacaaatgt tgtcacagct
     6241 cctttttcag aataaacggt gtttaagaga cagccaatcg gcaataaggc tgaacgtctc
     6301 tttcacagcc tcttttttga tgagtcatca tacatttttt cgcttaaata cgagatatcc
     6361 taatgctaag aacaaaacga tgtaagctaa attaccgaat agcatttgat tagtagaaag
     6421 atgagtcaat tgcttcatga tctcttcgtt tccaatttgg ttttgcagat tcaacatact
     6481 gatcggattc catttgatcc actcccattt ttggatgagt gcaataagga taccagatat
     6541 gattgacgaa gcaaaataaa agacgattcc tgctgagatg gatgcgccgg aactattgat
     6601 aaagcaagca atcatcaaca ctaaacttaa aatcagccat aaaccgatat aactacccaa
     6661 agtataagtg aacaagcttt ggatcagtgt ttgaccagta gaaaccttct ccgtgaagga
     6721 aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca ataatagcta aaaggtataa
     6781 ataaacagaa taaataacta atgtgatcca tttgcttact aatatttctc ctcgagaata
     6841 ctttcgataa aggagatttt tcaatgtacc atattgtgct tccatcgaaa tgatcgtgct
     6901 tgcagctgca atcatgataa agacaatcca agatgtggca ctaaaaagct gagcagtcat
     6961 ttcaactgga tcaatgatcg tcgtgtattt tttggctaga agtgctgttc ctatgagcaa
     7021 aacaactaat agaacagata aaacagcagt tgactttttc ttgatcaatt taaataattc
     7081 ttgttttaca agcaatgtca tttttcttct tcccccttta ctgaatccag aatttccaat
     7141 agagaagatt ccaaatcatt cgtcagatgc tggacgtctg tcacatggat attttgtgca
     7201 gacaatagct ggatcacggt tcctaataat tcttcgtttt cttttaaaag cttgatttta
     7261 tcaggatctt ccaaagtata tccagcttct gtcaagatcg ttcgagcagc tagatcattt
     7321 gttgtagaga taacgatgaa atcttgcgct gtttggttca attcagccat tgtacattgt
     7381 ttgatgatct ttccttgatt gatcaggatc agctcatcga ccattttttc aagctcgctg
     7441 agtaagtgac tggagatcaa gaaagaggtg ccctgagtag ccaagtcggt aatgattttt
     7501 cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg gctcatctaa aataacgata
     7561 gacggatggt tcagtaaggc aagagcaatt cccaattttt gtttcattcc caacgagtag
     7621 gatttcgctt ttttgtgaat gtacttttcc atcttgagct gattcacgat agtgtcgata
     7681 gcagatacat cgttggtttc agagaataac ttcagatgat catagcctgt taagaatgga
     7741 tagatacccg ggtattcgat caatgctcct acttgttcca accctttatg actagaggga
     7801 gaaatctcat tttcaccaat acagattgtt ccatctgagt aatggatcag tcctaaaata
     7861 gctttcatga tcgttgtttt tccagcgcca ttaggaccta ctaatccaac gattttgcca
     7921 ggggataagc taaaactgac atcgctgagg gcttgtttgt gaccaaagtt cttggataat
     7981 tcttggaccg aaagactttt caaaaatatt cactcctttt ctatagatag tacaagcata
     8041 cacctgatga gtggagaagt cgctaggtca ttggacttat ttcttatttt ttcataaaaa
     8101 aattgaatat taaataaaaa ggcttatttt ttctgatcag tacttattcc aagtgtctag
     8161 tggccgctag taatcttgat gccaatgata ctgatgagta gcatccccac aaagaaccaa
     8221 gtggaaagat tcagcttatc atgaaacaag accacaccaa ttatgaccga accaactgcg
     8281 ccaatccctg tccagatagg ataagagaga ctcatcggca aacttttgat tgcctttgac
     8341 aaaaagtaaa aactagcaat catgccaatc actgtgtaaa aagaataatt gattttagaa
     8401 aaaccatcac tccatttcat catcgttgac cagaatactt ccaaaattcc tgctatgatt
     8461 aattccatcc atgccatact tacattcctc cttaactata aaaaaataca caaaaagagg
     8521 aaaatcatcc cttcaaagac ctaatggaat gatcatcctc tttatcctac ccggtggcag
     8581 attgtatatc tttttctgta gtatagcaca cctatccaga tatcggcaat ctcatacttt
     8641 ttagtaaagt gtaccaagca ctgacggata tatcgttagc ggaaaagaag atgaatgctt
     8701 tgcaatttca tagaaagaag ttgttattta ttaatcattt aaaaaaaaca
//
LOCUS       DADVXO010000104         8739 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.107, whole genome shotgun sequence.
ACCESSION   DADVXO010000104 DADVXO010000000
VERSION     DADVXO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.107"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1166
                     /locus_tag="KB950_002206"
     CDS             <1..1166
                     /locus_tag="KB950_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6858391.1"
                     /translation="CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVTRLKKQ
                     RYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTR
                     TLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLP
                     RLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELR
                     IREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMI
                     DRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYE
                     EGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1345..1674)
                     /locus_tag="KB950_002207"
     CDS             complement(1345..1674)
                     /locus_tag="KB950_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6858392.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1664..1936)
                     /locus_tag="KB950_002208"
     CDS             complement(1664..1936)
                     /locus_tag="KB950_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBB6858393.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2387..3190)
                     /locus_tag="KB950_002209"
     CDS             complement(2387..3190)
                     /locus_tag="KB950_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6858394.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3168..4292)
                     /locus_tag="KB950_002210"
     CDS             complement(3168..4292)
                     /locus_tag="KB950_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858395.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4308..5123)
                     /locus_tag="KB950_002211"
     CDS             complement(4308..5123)
                     /locus_tag="KB950_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858396.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5113..5985)
                     /locus_tag="KB950_002212"
     CDS             complement(5113..5985)
                     /locus_tag="KB950_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858397.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6070..7359)
                     /locus_tag="KB950_002213"
     CDS             complement(6070..7359)
                     /locus_tag="KB950_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6858398.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7587..8231)
                     /locus_tag="KB950_002214"
                     /pseudo
     CDS             complement(7587..8231)
                     /locus_tag="KB950_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2712 a         1855 c         1391 g         2781 t
ORIGIN      
        1 cttgtagaag ttgtggttct actgtggtag atggaaacgg gaaagcaatt atagtgaaaa
       61 atgggaagaa ggaaacgatt gtccgttttg aacaatacaa tcatatgcct ttggttacgc
      121 gcctaaaaaa gcaacgctat acctgtaaaa actgtcgtac ccattggacg gctcaaagtt
      181 attttgtccg actcagccat tcaatcgcag atcatgttcg atataaaatt gcttctttac
      241 tgactgaaaa agtatctttg tcttttattg cgaaaagttg tcaggtatct ttgactaccg
      301 ttactcgtac attgaaagag tttaaaagct atttaccaaa gccttctaag aagattctcc
      361 ccagagtatt gatggttgat gaatttcgtt cgcacgcttc catagaagat aaaatgagct
      421 ttatttgcgc agatggtgaa acaggaaact taattgctgt cttgcctacg cgtaaattac
      481 ctcgattaac aagctatttc ttaggctgca ccaatccaga agaagtagaa ttcttagtca
      541 cagacatgaa cgccgcttac ttccagctta ccaaacgtgt tctgccaaat gcgaaagtag
      601 tgattgatcg gtttcatatt gtcaaacaca tgaatcaggc actcaatgag ttgcgtatcc
      661 gtgaaatgaa tgagcttcgt aaagcaggac agaaaagtca ggcagaaaaa ctgaaaaaga
      721 actggcgctt tctgctaaaa aatcgtgcaa gcatcaacca ttatgagtac aaaacatgga
      781 aaagtttccg agcaccaaaa tacccatttc tcactgaagc aatgatgatt gatcgattgc
      841 ttgagttttc tgcgcctcta aaggaggcgt atcccttttt tcatgaatta gtcgaagcct
      901 ttcgagacaa agaccctgac ttatttttct ccttattggc agaacttccc gaaacgttag
      961 atgacggctt tcgggagaag cttcaaaacc ttctgaccta tgaagaaggt atcaccagcg
     1021 caatgatcta tccttattcc aatggaaaaa ttgaagcgaa gaatacccac ataaagacaa
     1081 tgaaacgagt atcctacgga tttaaatcat tcgagaacat gagaattaga atctttttga
     1141 tcaatcaatt aatcaatgta agataacaaa aaatctgaac cagaatgtgg ttcattctga
     1201 cccggatttg attttgcaca actcatcagt ccttattgac aaagagccaa aaatctgagc
     1261 cagaatgagg ttcattctga cccagatttg attttgtaca acccatcagt ccttattgac
     1321 aaagagccct tctttttagt attattataa ttcttccatg cgttcttcca gtcgctgtaa
     1381 atcgcttgga cttaattccc caattttttt ccatttgacc tttttgttgt ttttgggtaa
     1441 ttcgtttaga gttcctacgt caatccaaga ttcttttttt agtcctgcct gtttccaatc
     1501 ccttatttca acgtatttca gctttatttt atcggattta gtatgatatt ttgaagtaat
     1561 tgacagaaac gaaattgtgt ccagtccatt ttttagaata tagacaggcc ggcgtttacc
     1621 gccctctgca gctgtgaaag caacgtagac ggttgctata tcattagtat tcatcttcat
     1681 tgaaccattc ttcaattttt tcgtcagtgt ctagtttttg gacaggcaat ttttccgcta
     1741 atctttgaat attatggatt cgtttacttt tttccgacaa ttcaatagaa aatgggattt
     1801 ttccttgctc aacaatttgc ttataaaaaa cggtgacggc tgttttgggg ctcatgccta
     1861 gttcatcaat aatgctttct gcttcatttt tcaatttttc gtccatagaa atttgtaact
     1921 tagccatttt ctccacctct ttctgacaaa cacattatat cacggaaaga gttcaaatgg
     1981 aattaataca actacttttg aactccttga aatgcagtgt atttatctaa atataaggaa
     2041 aggatatagt gctaaatggc cttgatagat agaaaagttt caaaatccgc cacagcgata
     2101 attgaagcga acactcaacg acacacagaa caggaaaaga ctcctaaaag aaccaaatta
     2161 actaggacgt gtctgaaaac tccacaaaga tgtggtatgc tctggtaaaa aacgaatggc
     2221 acaaccacga agaccgatat ggttcttgga aaactttctc cacccttttt gtaattggag
     2281 ggatagtggt ttattgatag cgattttcca agttcttcaa gtggaaccag acattgagca
     2341 ccacggctaa aaagtgaatg aagtctttat ttttaatcaa taatttttaa tgattaccag
     2401 taattggttt tttcatgcgc cctatttcta tttgatgtgg agttaaataa tgctgataag
     2461 taaaaatttt tccgtcaatt aaactgccac tagtgaatcc tgttctattg gtaaaaagag
     2521 aatagtctgg gtattcatct acaccaaatg ataaagattc agccataaac tgttttttac
     2581 cagcaaattc agcactgcta ccttgatgga tatgtcctac aaaaatacct aaaatatcac
     2641 tttcggcaat caattcagca aaaccttccg gaacttcagt tacaatagat ggatcttccc
     2701 acgacaatgg atgatgaacc aataaaatcg ttcccttttc agcaggtgta cccaaccact
     2761 cttgtaattg aatcatctgt cctatcgata tttttccttc gtgctgggta tcttgagcag
     2821 aatccaagga aataattcgt aacccatttt gattcgtaca atggatatac tctcccgttt
     2881 cattgctttc aacactcatt ccttcaaaga aaacactgcg acgatcatga ttcccacgac
     2941 aaaaatagta aggtgtggtt gggaaatatt gatccaacat tttccgtaag acttgataat
     3001 cagaaatccc cccttcatgt actaaatcac ctgttaatag aataaaatcg tacactgtta
     3061 acgagagttc ttgtaacaat ttttccaatg ccaactgtgg atttttaatt gttggaaata
     3121 gttcagcagt aaacaagtta gtttgatagt tttctctaaa atggatatca gagagatgca
     3181 aaaagcgcat ggctattccc tctttcttca ataaaataat caggatatcc taaattttct
     3241 tgcgtctgat aatcaaaaaa atgcattctt tcaggttcta tgcggagatt gactgcatca
     3301 ccaatctgcc aagtaggttt ttcattaagg acaatcattt ctataccatc gactttaacg
     3361 gtgtacgcat aactttgacc taatagctca cgatacttca cttgaccgtg taaatcgctt
     3421 gtttcttttg aaaaggttac gtgttctggt cttatgccta aatacaatga gccactaacc
     3481 tgaagaagtt gtttttgcca agctggattc aatgttaaaa attgactggc tacctttaat
     3541 gttccttgat cataagtaac tggcataata ttcatgcctg gcgaaccaat aaaatttgca
     3601 gtaaaagtat ttttaggttt cgtataaaca tttttcggcg tatcaatcat ctgaattcta
     3661 ccttcattca ataaaacaat ccggtcagct aacgtcatcg cttcaatttg atcatgagtg
     3721 acgtagacaa aagtttgttt ataacgttga tgaattttta ccaattcttt tcgtgcatcc
     3781 aatcgtaaac gcacatccaa atttgataac ggttcatcta ataaaaaata attactccgt
     3841 ttaaccaccg cacgagccaa tgctacacgc tgccgttgcc ctcctgacaa atctttagga
     3901 agtcgatctt ggtatggcgt taaacctaac atcttcaaag cttcttccaa acggtgctga
     3961 atgtcagctg tcttaatttt attcgccctt aatgcaaaaa taatattctc ctttacagtc
     4021 atgtgaggat ataaagcata attttgaaaa accattgcga tctctcgttc tccacaaggt
     4081 agcttatttg ctagttgtgt acctaaaaaa agctctccac tcgtaatttt ttccaaacca
     4141 gcaatcattc tcagcaaagt agattttcct gaaccagatg gtcccagtaa cacaattcgt
     4201 tctccagcgg ccacagctaa atcaatttct tttaaaatgg tatgttctcc atattttttt
     4261 ccgatatttc ttaattcaag cgtttctccc atgatttgtc ctccaattta tttaatacct
     4321 gcagaaacaa aagtcccgat aatatatttt tgaaaaattg catacaagac taacggtaca
     4381 aacattgtaa ttaaactaat cgccattgct acggtaaaat ttgtcccacc ttcagaacta
     4441 atataggtct gcatcgccaa cggcaaagta taattatcta ctgtccgtaa aattaatacc
     4501 ggccaaacat actcattcca agaggtaata aaaaaccaaa taccggtaga agtaatcgca
     4561 tgtctagtaa gaggaaaaac aattttactc atgacggttg tttgtttgat gttatccaat
     4621 gccgccacat ctagaaggct cactggaatt tcccgcattg tttgcgtcaa taaaaaaatg
     4681 cccatcgcat cagcaaattg tggtattatc acaccccaag tagtatcaat taaattaagt
     4741 tttgcaatca acaaataatt aggaatcatc gcaacagtaa aaggaatgaa catcgtacca
     4801 atgatgataa agtaccagat tttcttccct ttgattcggc aataagtaaa actatatgcc
     4861 gcaaaaaaag ctacgcctaa cttaacaacc gttataattg tagcaataaa aaaagtattt
     4921 ctcgtaattt tgataattgg aagattttcg aacaaatagc aaaaattttc caaggtaaac
     4981 ggctgtggta tcaaactagt aatcgtctga taggcatctt tcaatggctt aaaagaattg
     5041 gccataataa acaataatgg atagataata atgattatga tgaacataaa taagagatgt
     5101 ggcaaccagc gtttaatttt catagtaaac ccccttttcc acaaatttaa atattacccc
     5161 taagaaaaca gcaaatatta ataaagttaa aactgaaatt gctgaagatt ctccgacttt
     5221 atacatttca aaagcctttt gataagcata atagatcacg tttgcactac cattattcgg
     5281 tccaccacga gtgatgatct taatcggagt aaacacatac tgcagacctt gaacaatggt
     5341 catcaccaat aaatatagtc ctgtcgaact actcataggc aacacaattg taaaaaaaat
     5401 gcgccagatg ggtacacgat ccaacttagc tgcttcgatc atctcttgat caattcccac
     5461 aattgctgca aaaatgacaa tgaaattata accaaaaact ttccaagcag taatacctgc
     5521 aatcacagca attacccaac cttgtaaatt catccacata ggcagtctta aaccaaaatt
     5581 tcccaacaag acagcaattg gtcccgatac cggatttaat atccatgtaa aaaccatccc
     5641 cgccactacc aaagaaatca cagctggaat aaaaaataat ggtttataca aattctgccc
     5701 tttttctata actattccta ataaaaagga tgtaatatat gggaacacac agctcagcaa
     5761 gacaaataga acggtatagg acaaagtatt cttcaacact ttatgaaaga ctggatcagt
     5821 cagcaccttt acatagttgg caaagccaac aaattcttta gtgggggcca ccatattcca
     5881 gtcaaaaaaa ctcaaataga tcgtataaat caatggataa aaaacaaaca cgcccaacaa
     5941 aataattgct ggcgccagat acaaataacc tacatagcgc ttcatttccg acagcctcct
     6001 catccttatg tcaaaagcag acgaccttgt ttttcctttc ttacaggcgc tgtcgtctgc
     6061 ttgctttgct tatttgagta actcattaat ttccttttga gtagctttca tcgtttcatt
     6121 gacggaatta cctgataaaa ttttatcccg catatccaac aatttttgtt cagcttctaa
     6181 tccagcatct ccaggaaatg aactccaact gaccatctta tccatctgat caattgctgg
     6241 tatcatcatc tgattttcag ctaagaaagc ttttaatcca ttttgggcgt ctgcaacatc
     6301 tttacgtggt ggtaaataac cagtaccttc tgtccattta gctaaagatt caacactata
     6361 aagatatttt aagaaatccc aagttgcttc ctttttctta tccgtttgtg atgtaacagc
     6421 taacattgcg ccaccagcag gtagtttaac ttttttccct tcccaacttg gtgactcaac
     6481 tgctttagca gtaaaagtac tatttttttg aatgttgtcc ctttgagcaa tagtggtata
     6541 gaccatcgcg acgttaccat caataaagct ttgcacacct tgttcccacg tagtgtgtaa
     6601 tgccgtctgg tcttttacca tcctttgata taattcataa gcagcgatac catctttaga
     6661 agcaaaggta gctttgccat cttttattac acttgcccca ttactttcta aaattgcttg
     6721 ttgtgcccaa ttatcagcag gttcttgaat atacacgcca tactttccag ttttttcttt
     6781 tattatctta gaaaaagttt gaacttcttc ccaagttttt ggaccatttt catccaagcc
     6841 cgctgctttt aatatatctt gatttaaata taaaaccgga ttactaatgg agtaaggaat
     6901 acccacttgt ttaccatctt tttcagacag tgccatgata ttgtctaaaa aatgatcttt
     6961 aaaataatca gcattatccg gatccacttc ttctactaaa gattgaggat tagtatattt
     7021 gaaattttct gcaaaataat ctttaaaagc ccatcccact tgtactacgt ccggtatttc
     7081 tttggaggca acagaagctt gtaaattttg catcaagcct ttatacatat caggattata
     7141 ttttgctaca accttcactt tgtcttgaga ttgattgtat ttatctacta attcattcac
     7201 tgtttttcca cctagggtat ccgcattaac atgccaatac tctatcgtta cacgaccctc
     7261 cgattttttt gttgtcttgg tattcccact accacaagct gccaaagaga tgacacttaa
     7321 aacagacaca acccctataa caagattttt ctttaacata acattaactt cctcctaatt
     7381 tctttatggc catttttcta atcaagaaat attatgaact accaatgtaa atttctagtt
     7441 aattttctgt aaataattcg aaatttgtgc atttcggggt acagggtcac tggacatatc
     7501 catcaagcta agaataaagc ccccatatgg tcatgtatcc catctccaaa tctcttaact
     7561 cctctgtttt gattataaaa tagtttattc tgtgctcctt tcttccataa ataggcccat
     7621 gcacaacgtg aggttatatt ataaacacca cagcgcgcaa ggtactcaat cgtttctagt
     7681 tcattgactg tccatacaga aaatgtcaat cctagctgct gccccacttc acgggccaac
     7741 ttagttaacc atccttcatg aatattgacg atttgaacgc tttgataggc taatttctgt
     7801 aaggtatcga tcaaatctgc ttcggtaata tccccaagtt ttttcccttc gaactgattt
     7861 tcaatattat aaaatatatg ctttccccaa atcgcagccc aagataattg tacatagcca
     7921 ctaagaacaa tttgtggcca gatttgatag gtttgtgcca gcgaaaaaac ggccatttcc
     7981 aatccctctt cttttaaatc acagttaatg gtcatggttg ccttctcatt ggccactgct
     8041 tgaaacacct gctcaagggt taccgaatct gggacaactt cttacctgtc atgggctagt
     8101 attagcgtac catctgtttg ctgacgcaca tctacttcaa aacaaagctg gcgatccaaa
     8161 caaaagcgaa gaaatgccca gctattctcc tccgtattgt cactaccaga gtgtgcagtg
     8221 atcatggtca ttttgacccc tcctttaatt ccgtgatttt ctcccacacg tccactatac
     8281 cgatgaagcg ttaatttctt gttaatttct tgtaaattcc ctgcgttttt tcagtaaact
     8341 agttgtaaat cttgcatgct ccttcctacg aactcatgaa tcgtcgattt tgcccttcta
     8401 gctagtttca ccaattaagt agttctacat tccccgcttt taacctacta cacctttttt
     8461 tgttttcagc tattccttct tcttgctgaa agtccttgag gttctttctt acaggatacc
     8521 atcaaggtgt tcaatggctt attcccattg aatgaccgag acttactatc agatttctgc
     8581 tcgttgaacc aagtctttga tacaggattt cttccgtcat cttctcacaa ataccacctt
     8641 gcgtttctct agcgattggt cgatgtgcac cctcccagtg gactgtcact accccttttt
     8701 gctatgccta gcccacaaaa aaagctgtcc tcacagaca
//
LOCUS       DADVXO010000105         8677 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.108, whole genome shotgun sequence.
ACCESSION   DADVXO010000105 DADVXO010000000
VERSION     DADVXO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.108"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..475
                     /locus_tag="KB950_002215"
     CDS             <1..475
                     /locus_tag="KB950_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6858399.1"
                     /translation="DKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHLID
                     VIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKYGR
                     KVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            568..1730
                     /locus_tag="KB950_002216"
     CDS             join(568..844,844..1730)
                     /locus_tag="KB950_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB6858400.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2010..2990
                     /locus_tag="KB950_002217"
     CDS             2010..2990
                     /locus_tag="KB950_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6858401.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3112..4530
                     /locus_tag="KB950_002218"
     CDS             3112..4530
                     /locus_tag="KB950_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBB6858402.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4601..6070
                     /locus_tag="KB950_002219"
     CDS             4601..6070
                     /locus_tag="KB950_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6858403.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6080..6547
                     /locus_tag="KB950_002220"
     CDS             6080..6547
                     /locus_tag="KB950_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6858404.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6583..7455
                     /locus_tag="KB950_002221"
     CDS             6583..7455
                     /locus_tag="KB950_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6858405.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7536..>8677
                     /locus_tag="KB950_002222"
     CDS             7536..>8677
                     /locus_tag="KB950_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6858406.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECA"
BASE COUNT         3000 a         1300 c         1756 g         2621 t
ORIGIN      
        1 tgataaaatt cagtttaatc aagttataga agaattcatc aaagtaaata atttaatgac
       61 ccatattcaa aaacgaccaa tcagaataac taatgatcta aaactcagta cgagtatgat
      121 tcatttgatt gatgtgattg gaaattatcc tgaaactagt attacggaat tagctgatcg
      181 attaggcgtt accaaaggag ctgtatccca acagattccc actttagaga aaatgaatct
      241 tataagcatt tctcaaaaaa aaggaaataa gaaaactaaa cttgtatcgt taacaaaata
      301 tggtcgaaag gtgtacgaat cacataatta tttacatgaa gagctttata gctcaataaa
      361 tgaagcgtta gaatcattct caccagacca aatttcaacc attaaacaga ttctagaaaa
      421 agtatcttat acaatcaatg aatatcaagt aagtctgaca agaaaggaag attgacatga
      481 aaaaaatgat ttttgaatcg gctacactag actagacaga aaaaataagg tgtgtaaaat
      541 aaatgaaaac acactggagg aatttttatg tcaagacgtc aacgtagaac ctattcaaaa
      601 gaattcaaac aacaagtcgt cgatctttat ctcgcaggaa agccccgagc tgaaatcatt
      661 cgagattatg agctgactac ctcggccttt gataagtggg tcaagcaagc gcaaactact
      721 ggctcgtttc aggaaaaaga taacttgatc cctgaacaag tagaactaat tgcattacga
      781 aagaaaaata aacagcttga aatggagaat gacattttaa agcaagcggc gctgatattg
      841 ggacgaaaag acaagtaatt gataccaata agcacaaata ctcaatatca gcgatgtgta
      901 aagtgttaaa catctcttgc caaacctatt attatcaagc caaaccagtt gaagatgaat
      961 cagaactgga agaactcgtt caagaagaat ttattcgcaa tcgaaaagct tatggtacca
     1021 ggaagctgaa gcagtgcttg gccaatctag gtcttcaagt gagtcaacgc agaatcggcc
     1081 gtatgatgaa acagcgtggc ttgcgttcaa catacacatt ggctcatttc aaattccatt
     1141 cttctacttg taacgaagcc aagaccgcca atattctaga cggaaccttc ctacaggaaa
     1201 agtcattaga agcgattgta accgacctta cctatgttcg tgttgggaag aaatggcagt
     1261 atatctgcct gattcttgat ttgtttaatc gagaaatcat cggctattca tgcggggaga
     1321 agaaagatgc gacgttagtc aaggaagctt ttgcaaagat tccgtactcg ttgaataatg
     1381 tacaactttt tcacacggat cgtggaaaag aattcgataa ccagacgatt gacgaaattt
     1441 tatgggcctt taatatcacg cgttccttaa gcaaaaaagg ctgcccctat gataacgccg
     1501 ttgcagaatc cacctataaa tctgtcaaag tagaattcgt tcatcaatac cgctttgaga
     1561 cattggctaa tttgcgatta gagctatttg attacgtcca ttggtggaat accatccggc
     1621 tgcacggcac gttaggctat gagacaccga tcagttttcg acagcagaga ttggcgcagc
     1681 gaatccttga taatgagctc ggatgtgata ccggtggcga ggcaccttaa ttatttgaat
     1741 gagcttctgc cttagagaat cagattcgag gaggctcgtt gtcaagggca gtcggaacac
     1801 cagcagcaag cttacgcaat accttataat ttttgtcaaa aaaagtgttg ccattccaca
     1861 tatatcttta aacagctatt gccagataaa aataaatgat attatatata taaatactta
     1921 tccgaaaagg ggaatgagag agtcaaggtt cttggaatgt cgaatagtcg agtaggtaga
     1981 ttttctatct acaaccttgt tgtttaaaaa tgaaaaaaat tggaattaga gaagttgcga
     2041 aagaggcaaa tgtttctcct acgactgtgt cgagggtatt gaatgatcgt ggatatttga
     2101 gtgaggaaac aaaaagaaaa gtttttgatg ccatgaaaaa attagaatac tatcctaatg
     2161 aactggcgag agcacttttt aaaaacgaaa catataccgt aggtttgatt tttcctacaa
     2221 ttactaatcc atttcacgct gagttaattc aagatatcga gttcgagctt tccagcaggg
     2281 gatataaagt gttgctgtgc aatagcttaa acaatccaac aaaagaaaaa gagtacttga
     2341 cgatgttgag aaagaatcag gttgatggaa ttatagtagg aacgcacaat aaagatattg
     2401 cggattatga tattccaggg ctacctattg tagcgattga tagaagtctt ggtgaaaata
     2461 cagttacagt atcttgtgat aattacaggg gtggggaatt agctactcaa ctacttgtaa
     2521 atcatggctg tcagaatata ctgtgtattc ggggagatag taagataaag ctacccgcga
     2581 atagtaggac tttagcatat aaagatgttt tgaataaatt catgctggaa ccgttagtcc
     2641 tagaagtacc atttgtaaaa gaaatcaatg aaaaaaatga aataattaag acatatctgg
     2701 aaactcatcc aaatattgac ggggtttttg caggagatga tttattggct tctttggcga
     2761 ttaacaactt aggaagttcg ggcaaaagag ttccaaaaga ggttaaagtg attggatttg
     2821 atggagctca acaaacattg atttataatc cagaattatc aactattcaa cagcctatag
     2881 atcaaatatc taaagtagca gtaaaaaaat tattgaatat gattaagggc gaaaaggaga
     2941 cggatgaact ttcacttcca gtaaagctca taaaaaaaaa tacagcataa gttaaatcgg
     3001 cgaaaaattc cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca tatgaaacgg
     3061 gttgacatat attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa
     3121 ttattaatta aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga
     3181 gatgttatgc cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga
     3241 aatccagttc cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt
     3301 cattttgaag actttgggga atctctttta agaggggatg acaatgagag agaccagttt
     3361 atctacgctg gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat
     3421 aatcgtgaat tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat
     3481 gattttgttc attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat
     3541 gatattcgaa actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg
     3601 ctaattttag gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa
     3661 tttacatcaa aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg
     3721 tataccatgt ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat
     3781 tcagaatact cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct
     3841 tggattaagc ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct
     3901 tatgatggaa aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat
     3961 aaaaacaatt atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat
     4021 ttttcacttg gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat
     4081 attgccgatg tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat
     4141 agtggaactt tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt
     4201 tcactgagat tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta
     4261 gatgaggggc gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat
     4321 aaaggactta ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt
     4381 gttgatgaca atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat
     4441 ggttgttttg gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat
     4501 accaagtttt caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa
     4561 tcaaacatga cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc
     4621 cgaagatgta ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat
     4681 tactcgttta cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga
     4741 aatagaaggt gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac
     4801 aggtataatt gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc
     4861 cgtttctgaa atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt
     4921 taaagttttc tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt
     4981 tttaggaatt cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc
     5041 tgatcacaat gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt
     5101 tgattattta ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat
     5161 tttaggatta cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt
     5221 cgtcatggga actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt
     5281 tgcgtttcaa gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga
     5341 gaaaattaca aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac
     5401 aattgttctt gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg
     5461 taatgtttta ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt
     5521 tgtttttgca ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg
     5581 tattgaggca aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt
     5641 ttcaattatt gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc
     5701 taaagataga gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc
     5761 ggctatcttt gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg
     5821 tgctggtttt ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt
     5881 gactgtattg ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct
     5941 catagcattc attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat
     6001 ccctgaagct aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca
     6061 agtaaagtga gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc
     6121 acctgcaacc ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg
     6181 aatgatggga caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga
     6241 tggttttgtt tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg
     6301 aattgagata cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga
     6361 tattaaagtt caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt
     6421 tgtagttttt gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca
     6481 agacgaaatt ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct
     6541 tggctaatga taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt
     6601 gaagctggtg gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa
     6661 aaagtcagct ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt
     6721 aatcatcata aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt
     6781 cattgtgaat ctaaaacgta tggcttcatt aaatcaacgc caaaacttgc ttggcaggat
     6841 tttgatttta ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat
     6901 gtaaatgcct cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt
     6961 gtttactata ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt
     7021 gaaggtttta gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac
     7081 ttttctggga catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca
     7141 atcgaaagaa gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa
     7201 atagaagctt attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat
     7261 gtcattatat ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat
     7321 aagtttattg agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg
     7381 acaccaaaat tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt
     7441 aaagaaaaat cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc
     7501 tttttttcaa ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg aaagatgaag
     7561 tattagagta tatcaaaaat aatttaaagt attacgattt ttctgctcaa gatattgcaa
     7621 tgaaattttg tattaaaaga aatgtcgctt cccactattt gaatcagtta ttttcagatg
     7681 gaaaattact aaagaatgat tctgttagac cagtaatgtt caagtataat caacaaaagc
     7741 caaaagactg cttttctaag tttattggag cagacatatc tttgaaatcc actatagata
     7801 aatgcaaagc tactgtaatg tatcccccta atggcctccc attaataata aaaggaaaca
     7861 gtggcgttgg aaaaagtttt ttagcaagtt tgatctatca gtatgctctt gatagaaaag
     7921 taattcataa tgacgcaaaa tttgttgttg tgaattgtgc agattatgca aataatccag
     7981 agctattgtc agcagtttta tttggatata aaaaaggagc ctttactggg gctgaaaaag
     8041 atactgcagg gatgctatct tctgctaatg gaggatacct ttttttagat gaggttcaca
     8101 atctaagtgc agaaaaccaa gagaaattgt ttcttcttat ggattctcaa aagtatcgta
     8161 aacttggtga aagtgtgcaa tgggagtatg caaatgttcg ccttatctta gccacaacag
     8221 aagataaaaa tacctctttg ttagcaactt ttagaagacg catcccagct gaaatcactc
     8281 tgccagatta caataataga agtacatctg aaaaaattca attattattt gaatttctta
     8341 aagatgaagc aattacgatt gattcaaaaa tattttgttc gattgaactt tttactgatc
     8401 ttttatcgaa aacatttgaa ggaaatgttg gagaattgag gaatgaaatc aagcttcttt
     8461 gtgcagaagg ttatttagga aataagagaa agtcttcaat ttatttaggg gacaaactag
     8521 acagcggttt ttggatagat ccagaaatta gtatagattt aaaaaaaatg tttcttaaat
     8581 cactttctga aattgatttg attgaacttt ttcaaaatca catttcaatt gaagaaagca
     8641 tgagtcaatt aagagataga atacttaaag agtgtgc
//
LOCUS       DADVXO010000106         8488 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.109, whole genome shotgun sequence.
ACCESSION   DADVXO010000106 DADVXO010000000
VERSION     DADVXO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.109"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(68..358)
                     /locus_tag="KB950_002223"
     CDS             complement(68..358)
                     /locus_tag="KB950_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBB6858407.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(494..673)
                     /locus_tag="KB950_002224"
     CDS             complement(494..673)
                     /locus_tag="KB950_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858408.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(670..1020)
                     /locus_tag="KB950_002225"
     CDS             complement(670..1020)
                     /locus_tag="KB950_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858409.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1013..2338)
                     /locus_tag="KB950_002226"
     CDS             complement(1013..2338)
                     /locus_tag="KB950_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6858410.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2788..3402
                     /locus_tag="KB950_002227"
     CDS             2788..3402
                     /locus_tag="KB950_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6858411.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3567..3809)
                     /locus_tag="KB950_002228"
                     /pseudo
     CDS             complement(3567..3809)
                     /locus_tag="KB950_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4013..5332)
                     /locus_tag="KB950_002229"
     CDS             complement(4013..5332)
                     /locus_tag="KB950_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBB6858412.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(5487..6074)
                     /locus_tag="KB950_002230"
                     /pseudo
     CDS             complement(5487..6074)
                     /locus_tag="KB950_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6712..6969)
                     /locus_tag="KB950_002231"
     CDS             complement(6712..6969)
                     /locus_tag="KB950_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6858413.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(6962..7231)
                     /locus_tag="KB950_002232"
     CDS             complement(6962..7231)
                     /locus_tag="KB950_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6858414.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(7362..7541)
                     /locus_tag="KB950_002233"
     CDS             complement(7362..7541)
                     /locus_tag="KB950_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858415.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(7922..>8488)
                     /locus_tag="KB950_002234"
     CDS             complement(7922..>8488)
                     /locus_tag="KB950_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858416.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         2591 a         1601 c         1326 g         2970 t
ORIGIN      
        1 tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg
       61 attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt
      121 ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc
      181 ttaaatcata aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc
      241 ttgcaaccgt tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta
      301 aattcttgat aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg
      361 tgcgtaagcc tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat
      421 tctagtatct actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa
      481 tccgtaaaat accttatata tataacggga agatataaaa cctaaaatta tgataacctg
      541 ctgcagcttc tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc
      601 cataacccca aatctcttct gatagctgat caaaggttat atactttagc tcatattgct
      661 ggataatgtt cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat
      721 ttcgtcgtag tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat
      781 cttgccaatg atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga
      841 tactgatcta ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat
      901 ttgttctttt gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt
      961 atgatcagat aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat
     1021 ttttaattcc cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc
     1081 tagcgccttc taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa
     1141 tcttgtcaat aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata
     1201 aatccagctg aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta
     1261 cttcttgtcc ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg
     1321 tattatctgt gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg
     1381 acgtgccaat gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg
     1441 ctacttgttc cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc
     1501 gtggtaacac ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta
     1561 tatctgttcg atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc
     1621 tattcttaat cgtataagga ttccagttag caagatctct aatgctcata atccccatct
     1681 gattgagtct ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat
     1741 tccatacttt ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct
     1801 ttgcttcgtt gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag
     1861 caatcaatcc tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc
     1921 gttgacttcg tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg
     1981 attcatcgat cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat
     2041 ttacctgcat atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt
     2101 taggaaatgg ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt
     2161 ttgcttcagg agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa
     2221 caacaagttc tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa
     2281 agcttttcat atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa
     2341 ataacactcc attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag
     2401 ctaaatacat aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt
     2461 atgatcaatt tattctatca ctttactaat taatttgaaa atctaataaa aactctcact
     2521 ctaaaagagt gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat
     2581 attaacaaat gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca
     2641 aaaaattctc atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa
     2701 tagacactgg tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta
     2761 aatagttgca gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc
     2821 gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc
     2881 atcgagaaac aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat
     2941 tacgtccgag atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat
     3001 gacgagatca tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact
     3061 agtttaccga tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat
     3121 ctgatcatcc aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc
     3181 caggcacaag gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta
     3241 tacagtgcag acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat
     3301 cttaaaaatg gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc
     3361 gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta
     3421 ttatcttctc atctttaatt tcctacatat aattactatc tataaaatta agtcttactc
     3481 agaacagata aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct
     3541 gttaatataa tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct
     3601 atattttcta caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc
     3661 gacacattgg ttatactaat tcctatagct agttcttgag actctacttt tctctctagc
     3721 tcctttgaca aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct
     3781 tcataaaaat ctctatacat aaagctatcc ctgaataatt catagcttta attctttggc
     3841 acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac taaaaagtta
     3901 cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa ggcattttga
     3961 gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg gattagatcc
     4021 attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga tacacatgat
     4081 aactagataa tttgagatag actcgtctac ccgtttggac taatttccca gcaactttca
     4141 gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca aagccaatcg
     4201 ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg acatgattct
     4261 ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct tctttaatga
     4321 agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga gaaacatttt
     4381 ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct tcacgtgttg
     4441 agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg gtcgagaagt
     4501 actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat ttctcagcta
     4561 tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt tttgattcac
     4621 acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag atgtcagtgt
     4681 ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt acgccttttg
     4741 atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc aatccgtcaa
     4801 aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc gtttgttctt
     4861 ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac
     4921 ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct
     4981 cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat
     5041 ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt
     5101 aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat
     5161 acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt
     5221 tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca cctgtatgag
     5281 aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc atgaaaagaa
     5341 ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt cttttttcat
     5401 cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt
     5461 tttaattaaa agtatgaatt attcaggcta tcattaattt tataaaaaaa attaaatgtt
     5521 tttagcactg ccatataaca ttcaaaacat tcttctatag ttggtttact tccttgagga
     5581 gaatgaacca atttattacg tttttctatg aaatttttaa tttcctttct tatttctttc
     5641 ggaaactcaa tattaaagta ccctttaaac atgctcccta ataatttgtc tacaggggga
     5701 gattgtaaat tctcaaatat tagagttaaa tcaggtttct cttgttgata aaattccttt
     5761 acccctattt cacaggctgt aattatcgaa ataaacctat ctctagtatt tgatatattt
     5821 ttacttcttt taaggagtat aagagaaaag gggatacttt cgtgtccatg catgtaatta
     5881 actaattgtt gagcttcaga agttatatct ctaattatat agttaacgga attaggaagt
     5941 gtctttaact ctaatgaaat ttctatccaa ttactatctt ctaatctgta tatgctcgaa
     6001 gtataatttg attcgtcagc aagcatcgct acatcccaaa cactgatcat aaaatctttt
     6061 aaacgttcaa tcatttcagc aatattgcta aaatcagcta atttttcttt agaataaaaa
     6121 aaaacattta tatcagaatc aataaattct atttttttat tactatatcg tcatcattat
     6181 cttgaccatt tatgtttatt gttccctgag tggggagtac ttctttcaaa ttcaagttaa
     6241 atttatattc cattacttct cctccaatat ataaaaatta cttaactgta tccaaattta
     6301 atatgtaaat aaatcattaa tttttagcta aattctatga gaagtttatt tttgttgttt
     6361 ttgctaatta actaaaacta gtacaaaaaa tttaaaatag attattaaac ctattgctta
     6421 ttctattcag tcatatatat aagcgtcatc ctttagtaac taacatccca cactacaata
     6481 gtattataac atatctattc aacttcaaat tgttgaaatt taagccattt atatttactt
     6541 ttcctcaact ttagaaatga ttttactaac cttatatccg taatacgcgt aacaaacaca
     6601 ttcattaata acaatcaaca ctaagctata ttagcttttt aaatgactag ggtaaataaa
     6661 ataaggatca aaaactttta agtttctgac cctttcctta cttccgattg gttaatagtg
     6721 atcttttgca gaataaataa atatcgtttc attttcaact ctatatatca gtctatgttc
     6781 atctgtaatt cttctggacc attttccaga taaatcatgc tttaatggct caggttttcc
     6841 taatccagca aagggggaac gatcgatatc ttttattaac ttgttaatct tttttatatt
     6901 gcttttgttt ccttgctcat gccaataaag ataatcatcc caagcatcat cagaccaagc
     6961 cttaatcatc agattcaacc tcgattaagt catgtgtttt aaatgcacct ttggagaatt
     7021 gttcatctcc tcgacgaatt ttttccatga cgtaattatt agaaagtgtt ctcaacgttt
     7081 cttgcataga atcataatct ctttttgata atacaacaac tgtatcttct acatctttac
     7141 ttgttacaat aagtgtttca gcatcctcat taacttgttt catataacta cgtaaatttt
     7201 ggcggaaatt tgaataagct actgcttcca ttcctttcac cccttattta tttctataaa
     7261 acaattgtac attatattgt acaattaagc aaatgattaa tattctaact tcttataatt
     7321 aaaagtgcta tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt
     7381 acctcaaatc ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg
     7441 gattcacttt tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc
     7501 aacgtaacgg gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat
     7561 cgaaataacc ctatgaaaaa tatggctgca atcatcttga atagtagact cacaagggct
     7621 aatacaaagc gccaaaagat caaacaaaat accagtattg ccaaaaatac tacgaagttt
     7681 aaatgcacag ctgcagcatg gcgcataagt atttcgccaa caaataggac taacaataga
     7741 ggaatgattt tattcatatg gatctccttc tttctcttag gttatcgggt gttgcccgct
     7801 tatttcgcac cacctaagcg gcgggggcgg ttggagcgat tggtgcgaaa taagaaccat
     7861 tcccttagtt actaggtttt ttgtccttat ttcacacatt ctcacttttc ggtataaatg
     7921 attattgcct ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat
     7981 tttggatttc ttctgtatcg tcgtaagtat aggtgtctaa aaaatcagtt aaagtcgtgt
     8041 accagtcgtc gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta
     8101 cagcgagttc ataatccatt cccattaaca cgatagattc cgttccacac gtcaaaaatc
     8161 tagtaaatac aaggtaataa tcgtctatcg tcgcatcttt gctgtttagc tcttccattt
     8221 cttcttccat taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt
     8281 gtttcctgca aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa
     8341 cattcatgat gtatttttct gtttctagtc gtagaagctc cgttcctttt tttaccgttt
     8401 caggacgacg tttatacacg tctaaacgat tatggctgac tgtgaccttc gtttgactcg
     8461 tttcactttc caaatacaat tgttcttc
//
LOCUS       DADVXO010000107         8254 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.110, whole genome shotgun sequence.
ACCESSION   DADVXO010000107 DADVXO010000000
VERSION     DADVXO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.110"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            134..910
                     /locus_tag="KB950_002235"
     CDS             134..910
                     /locus_tag="KB950_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBB6858417.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            925..1128
                     /locus_tag="KB950_002236"
     CDS             925..1128
                     /locus_tag="KB950_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858418.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1144..1482
                     /locus_tag="KB950_002237"
     CDS             1144..1482
                     /locus_tag="KB950_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBB6858419.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1469..1648
                     /locus_tag="KB950_002238"
     CDS             1469..1648
                     /locus_tag="KB950_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858420.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1691..2161)
                     /locus_tag="KB950_002239"
     CDS             complement(1691..2161)
                     /locus_tag="KB950_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858421.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2248..2946
                     /locus_tag="KB950_002240"
     CDS             2248..2946
                     /locus_tag="KB950_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBB6858422.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3124..3465
                     /locus_tag="KB950_002241"
     CDS             3124..3465
                     /locus_tag="KB950_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858423.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            3458..4129
                     /locus_tag="KB950_002242"
     CDS             3458..4129
                     /locus_tag="KB950_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBB6858424.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4135..4827
                     /locus_tag="KB950_002243"
     CDS             4135..4827
                     /locus_tag="KB950_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858425.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            4824..5573
                     /locus_tag="KB950_002244"
     CDS             4824..5573
                     /locus_tag="KB950_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858426.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            5585..5854
                     /locus_tag="KB950_002245"
     CDS             5585..5854
                     /locus_tag="KB950_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858427.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            5860..6024
                     /locus_tag="KB950_002246"
     CDS             5860..6024
                     /locus_tag="KB950_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858428.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            6017..6316
                     /locus_tag="KB950_002247"
     CDS             6017..6316
                     /locus_tag="KB950_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858429.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            6316..6618
                     /locus_tag="KB950_002248"
     CDS             6316..6618
                     /locus_tag="KB950_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBB6858430.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            6658..7023
                     /locus_tag="KB950_002249"
     CDS             6658..7023
                     /locus_tag="KB950_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858431.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            7513..7653
                     /locus_tag="KB950_002250"
     CDS             7513..7653
                     /locus_tag="KB950_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858432.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            7650..7832
                     /locus_tag="KB950_002251"
     CDS             7650..7832
                     /locus_tag="KB950_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858433.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            7845..8039
                     /locus_tag="KB950_002252"
     CDS             7845..8039
                     /locus_tag="KB950_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858434.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(8072..>8254)
                     /locus_tag="KB950_002253"
     CDS             complement(8072..>8254)
                     /locus_tag="KB950_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858435.1"
                     /translation="EQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQLKYYSDYWTQ
                     TRENELNLEKLIIKKK"
BASE COUNT         3046 a         1260 c         1703 g         2245 t
ORIGIN      
        1 agctttaaaa tcaatgtttt tatgtcttat ttaattatgc tcttagtata gagtattact
       61 cagatactgt caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa
      121 aggaatgaga aatatgaaca caccgcaaat ttttaatttc gaacaaaacg aagttcggac
      181 aattttagta aatgatgaac catattttgt aggcaaagac gttgcgagtg ttttgggtta
      241 ttcaaacact aaagatgctt tgtcgcggca tgtagatttg gaagataaga tggggtcgcg
      301 aatcacgacc tcaggtcaat caagagagat gacaatcatc aacgaatctg gtttgtacag
      361 tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacaagtga
      421 agtgttgcca gcaattagaa aacatggagg ttatctaact ccagaaaaag tagaagaagc
      481 tttgcttaat ccagatacaa tcattcaatt agcaactcaa ctaaaagaag aaagaactgg
      541 aagattaatc gcagaacaaa agattgcaga gtacgaacct aaaatctcct atttagatag
      601 catattatct tctacagatt cagtaacaat tagtcagatt gcagcagatt atgggatgtc
      661 tccacaacag atgaataaat tacttcataa actaggtatt cagaaaaaag tcggtaacca
      721 atggttattg tgcaaaaaac acatgaacca aggatacaca aaatctcata caactgagat
      781 cccgaaagcc gatggtggca ctaaaattgt aatgaatacc aaatggacac agaaagggcg
      841 tctatttatc tacgaattac taaaaaaaga aggatattac cctcaaatgg atttagagga
      901 aattggttag aaaggagttt tagtatgact gacattgcag aaatcactca acgagataga
      961 gaaaaaatca aagaatatgt cgaaagttca aagttcttaa cttacaccat gcttgctgaa
     1021 agatttggaa ttagcaaaag ctacttatct ttaattttaa acggtaaaaa gacttctgca
     1081 gaagcaaaca gaattataga ttcgattatc actatgtacg aattgtaaag ggaggaacag
     1141 ctaatgcaat atctagaagc aaaaatccca attccagaag gctatgtaat tatctcccaa
     1201 gtggattatg aggagttaaa aaaagctgat gatactggta gatggatgac gttgccagaa
     1261 gtgctagaac ggattaacag aaaatatgat tggtttactt ctagagtttt aaagaaccca
     1321 agatatagaa atattatcga tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt
     1381 gaaggaagag acacatatct atttttaaga agtaaaacac ttgaattttt agaaacaaat
     1441 ttttcggaaa tcttaaggag gcaagcggat gggaaaattt aatagagcgc tagtgttcag
     1501 cgcaccgcta atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac
     1561 gattgtttac atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc
     1621 gaaaaagcca accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata
     1681 ttactattac tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga
     1741 tatcaacaat tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat
     1801 taaggagttt attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac
     1861 ttttaggagg aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc
     1921 ctcctttagc aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg
     1981 atgaaagttc taaattaact aacatagctg gttctgttga atagttagaa atgattactt
     2041 ttgtataaat catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc
     2101 gcattccttt tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca
     2161 tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa
     2221 attataccaa aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa
     2281 gctgtgacta cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta
     2341 agagatattg atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt
     2401 aatgaaccag attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt
     2461 ttcttgctgg ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt
     2521 gaagcattca acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa
     2581 gatatgatga tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt
     2641 aaagatacta tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg
     2701 aaagttatgg gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa
     2761 gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag
     2821 ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact
     2881 gcgatccgta tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat
     2941 gaatagagct gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta
     3001 taaaccaatc atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa
     3061 aaagtgactc cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag
     3121 catatggaaa aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag
     3181 ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa
     3241 ctagcttggg atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt
     3301 atccatctat cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg
     3361 attgatgcgc tagaagagta cgacagagat aacttgattg atatctctta ttacggacca
     3421 ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac
     3481 gaactttgaa aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt
     3541 tgattattta tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac
     3601 ggtaactatt aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct
     3661 tgttgaggaa tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt
     3721 aacggttaaa ggacacacgg aaaccgaatt atttcctgtt ttagattatc gaaaccaacc
     3781 agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc
     3841 attggctcta cacggactgg gattatatgt atttcaaggg gaagatattc caacaccacc
     3901 tagaatcgat acaaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca
     3961 gatgggtaaa gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt
     4021 agggctctta gctgataacg ttgaaactat tgaacagtta agctatgagc aatgtgcctt
     4081 gatggagcga gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt
     4141 aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataagc
     4201 gcaaccatca atgaggaagt gaacattgat cgactaaaga cgatgtatga cggctacgat
     4261 ggtgatcgag tcattgaaat tcgttttatt gatcctagac gtttcaccgt acagcaacga
     4321 aacttcatct atgcgctgat aggcgatatt tttatcgata caggcatgcc aacggacttc
     4381 tggaaggaat tcttctactt tcgttttgaa ggtgtcacag ggcgcaaaat aagcctgaaa
     4441 gacgaatcga atacaactgt gagtgatgcc aatgtcttag caaatatcat cttagatttc
     4501 atctttgaac atcatattcc tttcaaagaa ggctatgaga ttttacctgc gaatcaagaa
     4561 tattacttct acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa aacaggagct
     4621 gacatcgatc actttgacaa agcgctagga agacgaaagc gcaaagaagt tgatcatgca
     4681 gagtacacat ttgcagcact ctgcagaatc catcacacag agaagcacaa aataggtgtg
     4741 attaatttca aaaataaata tcaaatcaaa gggatcaaat taaaccagaa aacaatcaaa
     4801 aagttaaata ttggagggga aaaatgacag aacatcgaag ttattatgcg attataccag
     4861 ccaacgtaag gtacgacaaa agacttaaac caaatactaa gttgttatac ggagagataa
     4921 cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa tgagtacttt gcagatttat
     4981 atggtgtgaa taaagagacc atatcgcgat gggtaagtga tttgattaag tttggatact
     5041 tgaatcggga aatcatttac aaagagggta ccaatcaaat aatcaatagg tacctacgaa
     5101 ttaatcaata ccctattgac gaaaaacgca atacccctat tgacgaaaaa gtcaaagata
     5161 ataatacatc tattaataat acatttaata atacaaaaga atatataaga gagttaccgc
     5221 cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat aaaaatgttc
     5281 ttttgtcaga tgagcagatg gagaaactca aaacagaatt ccctaatgat taccaagagc
     5341 gaatcgaacg actgtcagag tattgtgaat catctggtaa gacttataaa aactatttgg
     5401 caactattcg aagttgggca aggaaagaaa aaagtgaacc taagaacgca agcagtggat
     5461 acaagcgcac aggaagacga gagaagcttc ctgaatgggc aatcgaccaa gaagcctatc
     5521 tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc taagaggtgg
     5581 aaaaatgaag atcgattatc tagaactaat taatgaaata gcgaattata aaaagggcga
     5641 ggaattagac gtcctgagag acgtatatga tcaactcgaa gaagctggaa ttgaaggaat
     5701 taagaatgat cgttcgagtt ggagtaaact cagatactat ttcgcactct atatcgatgg
     5761 aacgcaatta agaaatttag catataccaa attactattc attgattgtg ttaaaggctt
     5821 gcaaaaacat cttaatgaac ttgagcaggt gtaaacaaga tggacctaaa gacatttaca
     5881 gcacagatcg aattaatgca tcaagaagct ttaagacaaa gtgtgtcgta cgaagacaag
     5941 tggctcaaca catttcatgg cggacgtgag agcgcacttg atcaagtact caaattactg
     6001 aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg aattgactta
     6061 ttcagaagat tggcaggaag ataaaaaaat agttgcagaa gtccaaaagc tcggtaaatc
     6121 aatgtggact gaaaagccca aacggagaac gccgagaaag attgcaatct ggcatggtga
     6181 tcgaattcta gtaacgggta ccgctaaaca gttatctgaa attactggtc tgagcaaaaa
     6241 tattatttgg gatagagcta ggagcttatg gattgattca aaaggaagac agtttaggta
     6301 tgtggaggag aaataatgga cgaactaatc gcgaaagtag agcagtgggc aaaagataag
     6361 ggattggatc aagcggattt cagcaagcaa atgttaaaaa cggtcgaaga aatcggggaa
     6421 gttgccgctt ctctagctag aaaagatgaa catggtttaa gagacggaat tggcgatgta
     6481 gtagtaacct tgatcatctt ggcaatgcaa aatgatatgg atttatacga gtgcttggat
     6541 tttgcttatg acgaaatcaa aggtcggact ggaaagatgg tcaatggtgt tttcgttaag
     6601 tccagtgacc tgaaataatt ccattaatgg aagaaataac tgaataggag gatagacatg
     6661 attgatgcaa aacaagtgca aaaacagaaa gatggaatgt tgatgtttga agcatatgtt
     6721 ctaccatttc ttaatcaatt tgaagttttg gaatgctctg ccagcggaga agaattggag
     6781 tatgttgtaa ttcgtgaaac caaagaaaac gttcagaagc taaacgaatt tctttgcact
     6841 attaactgtt gggatatgat agctccagga tttttatgtc cagctatggg agaatttttg
     6901 gaatattgtc gtcttgaaga tgcaggcact ttggatttag cttatctggt atacaactac
     6961 ctaaacatca acacagatca tttgtggttt ggcactgccg aaagaaaatg ggtagtccgc
     7021 taaccaccaa tatcaatgaa aaaaggcggt ttcctgaaag tggaattgcc gccattgctg
     7081 taaggttctt tacgcgactc aaggtcacag aaatataaat gcaaatctct tttgaagtga
     7141 tccaaagctc tcggaaaatg gaagtttttt gtttcaaaga ataaatgaga tttgcgtcaa
     7201 gaacctgaga gttatttcca aaaaagcttc caaagaaccg aatgagctaa ggacatcatg
     7261 tgtaaacaca taatatagct ctcctttcac ggctaatcat aacatgaaaa atattcgatg
     7321 tcagtaggaa aaaaggtgaa ttatgaaaag aatattaaat tatcccggca gtaacagatt
     7381 ctgcaattaa actccgaagc tgatgtatca gtatcaactg tagaatatta tgagcgtgga
     7441 gaaagtcaag cagtcacaaa tgtaaaaatc gataacgtca acggtcttat ctgtttagat
     7501 gaaggcgata gtatgaatga aattgctgca gaccatgaat tatcagaaac tgctttaatt
     7561 gatcaaatcg aattagctag acaatacttt gttccatatg cagatacctg gtccaagcat
     7621 aagaatgaca tcatttttcc agaggaacaa tgagagcaac tgatccagta attatccttg
     7681 aggaagccaa atttatttgg actcacgaag agatagagca agcacgcttg ctcttttctc
     7741 aaggagttaa gccgagcaaa gtagctgaaa taatgggtca aaagattctt gatgtcggat
     7801 tgcttttgct ccatctagca gaaaaaattt gatttgaggt gaaaatgatg attcaacttg
     7861 caggcatcca aacagggaaa atttattttt ctggagaaag caaaagcgaa gccaaccaat
     7921 ggctattgaa aacttacacg aacaataaga agctaaggaa aaaatatcct gatttgttgc
     7981 taaaggatga tcagattatg ccggaaccga tgattttgac ccgtaaggag ggaagttgaa
     8041 actaaaaaac cagccgtgtg ggctggagtt gctatttctt tttaataatg agtttttcta
     8101 aatttaattc attctcacga gtttgcgtcc aataatcaga ataatatttt aattgtttaa
     8161 gagtaggaat gtcgtcttcg gtaaaattag aaaataattc tggagaatta tcttttttta
     8221 attgttctaa tcctgattct ttaaaacgtt gttc
//
LOCUS       DADVXO010000108         8194 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.111, whole genome shotgun sequence.
ACCESSION   DADVXO010000108 DADVXO010000000
VERSION     DADVXO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.111"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            589..1452
                     /locus_tag="KB950_002254"
     CDS             589..1452
                     /locus_tag="KB950_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6858436.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1581..3203)
                     /locus_tag="KB950_002255"
     CDS             complement(1581..3203)
                     /locus_tag="KB950_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6858437.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3452..3973
                     /locus_tag="KB950_002256"
     CDS             3452..3973
                     /locus_tag="KB950_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBB6858438.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4159..5889)
                     /gene="spxB"
                     /locus_tag="KB950_002257"
     CDS             complement(4159..5889)
                     /gene="spxB"
                     /locus_tag="KB950_002257"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBB6858439.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6054..6872
                     /locus_tag="KB950_002258"
     CDS             6054..6872
                     /locus_tag="KB950_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBB6858440.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6975..7172)
                     /locus_tag="KB950_002259"
     CDS             complement(6975..7172)
                     /locus_tag="KB950_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858441.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7341..7919
                     /locus_tag="KB950_002260"
     CDS             7341..7919
                     /locus_tag="KB950_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBB6858442.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            8014..8151
                     /locus_tag="KB950_002261"
     CDS             8014..8151
                     /locus_tag="KB950_002261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858443.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2446 a         1494 c         1615 g         2639 t
ORIGIN      
        1 tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat
       61 ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc tctccactaa
      121 acggtgttct cctgccaact cctcggtcac aagtcacaat attcaacaaa catgagaagc
      181 tgttttttga aattgttcct tgtgactcgg agctaaacgg caggctcaca acctcttggt
      241 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
      301 ctattttttc cgtttttttc tctaatactc aggtcaaaac gctgatttca caacctctga
      361 ttcacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
      421 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      481 tattttgatt agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg
      541 ctataataga cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt
      601 gtaacagatt cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt
      661 ttatcaatga atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc
      721 aattatgacc gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc
      781 aatgttggcc gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta
      841 tatgtggctt tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa
      901 ttgctgaagg aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt
      961 attggggaag ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc
     1021 gaagaaaccc tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta
     1081 gacgatctga aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc
     1141 agtctattga gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt
     1201 gacaaagttc gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca
     1261 atcgttgcac cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg
     1321 gagaaggcaa aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc
     1381 ttaggaccaa cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga
     1441 atcagtcgat aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa
     1501 aacagcgact cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat
     1561 tttttaagct aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca
     1621 ttttccaaaa attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg
     1681 gcaatgatgc ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt
     1741 gagccagtaa atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg
     1801 ttcgttggat catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg
     1861 caacggactt tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa
     1921 aacaacattc ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca
     1981 tattgacgca accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt
     2041 ggtaaataag aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt
     2101 tcacccatga taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg
     2161 accttgtctt ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc
     2221 gttacactta cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat
     2281 ccaacgaatg gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc
     2341 atttttttac gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata
     2401 aattcttgga gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta
     2461 gccgctagtt gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta
     2521 ccaaaatcta gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat
     2581 acaacgatca cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg
     2641 atatccagac cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt
     2701 tgtgctaata acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc
     2761 aattcttcag ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat
     2821 ccattaagct cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg
     2881 aaatcctctt tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt
     2941 cccatgatga ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat
     3001 acggccatcc gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct
     3061 gataacactt taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag
     3121 tttccggggg taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa
     3181 ctcacatcat ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa
     3241 gtcttataga attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg
     3301 tttcacgtga aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata
     3361 ggatttatta gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa
     3421 aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg
     3481 ctcattgatt ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt
     3541 cggaaattta gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt
     3601 tgaactgttt gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt
     3661 aataatcggg atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg
     3721 agtcgcagca ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt
     3781 atttactgct gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt
     3841 caatatttta ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt
     3901 gacattaagc tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta
     3961 tgcatttaga taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact
     4021 gttgtaattg gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt
     4081 gtcgagacaa atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact
     4141 cgaagcatag cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc
     4201 atggacttca tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc
     4261 taaatgcaga tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac
     4321 aggacgatct gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac
     4381 tgtaaagcta tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa
     4441 tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc
     4501 attcgaaaag acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg
     4561 catgaccata gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct
     4621 tagttgtgcg gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt
     4681 cgtatgtttt tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc
     4741 gacatctgtg acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc
     4801 ctctggacga atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt
     4861 ttctttattt tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc
     4921 gacaagtctt tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg
     4981 tcgtccaaat tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa
     5041 tgcacgtcca aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc
     5101 ttcatttccg ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc
     5161 aggaataatt ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa
     5221 ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt
     5281 tgctttttca agataaggca aagcagctag caaatctttt tcttctggta atatcacact
     5341 tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc
     5401 aacagggata gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc
     5461 tactacatgc ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc
     5521 gaaaatagga ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac
     5581 ttgtcctaag aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat
     5641 caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt
     5701 tgcatctgca gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat
     5761 ttcggttttt tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc
     5821 gtaaatatgg tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat
     5881 ttttgacatg gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag
     5941 tatagaactt tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt
     6001 ttttgggaat atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg
     6061 aatggctatt gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt
     6121 ttatgacagc tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc
     6181 taaacttgat gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt
     6241 tagatccggc tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta
     6301 ttgggtttgg tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc
     6361 attttggacc acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc
     6421 ttgttttttc tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg
     6481 gtgcagcagc aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc
     6541 taggtattgg tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag
     6601 aaggtctgag tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa
     6661 ttgctggagt gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac
     6721 ttgcttttgc cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac
     6781 agcagacatt gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca
     6841 ttatgatgat tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa
     6901 tagggccgcg aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg
     6961 aatggtatca tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca
     7021 agccagatga taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt
     7081 aggacactga caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg
     7141 aagatattta agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta
     7201 ctatagaaaa agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta
     7261 cttcatctac ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag
     7321 cggaaaagga ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa
     7381 tcgcgattat catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg
     7441 aggcgctcgc tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag
     7501 cgatttattg gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg
     7561 tcttatgggc gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat
     7621 ggatcagtga gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga
     7681 ccgctaatat tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg
     7741 agaaagcatt gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga
     7801 atgtgttagg aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca
     7861 tcgtgattct agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa
     7921 atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat
     7981 ccgagtgata ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga
     8041 ctgattacta gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca
     8101 gtagttggtg tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta
     8161 tgtaatctga tgttctatct atttaagaaa agca
//
LOCUS       DADVXO010000109         8060 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.112, whole genome shotgun sequence.
ACCESSION   DADVXO010000109 DADVXO010000000
VERSION     DADVXO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.112"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(9..530)
                     /locus_tag="KB950_002262"
     CDS             complement(9..530)
                     /locus_tag="KB950_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBB6858444.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="KB950_002263"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="KB950_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBB6858445.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="KB950_002264"
     CDS             4610..5497
                     /locus_tag="KB950_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBB6858446.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="KB950_002265"
     CDS             5494..6663
                     /locus_tag="KB950_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858447.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="KB950_002266"
     CDS             6687..8042
                     /locus_tag="KB950_002266"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBB6858448.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2590 a         1486 c         1828 g         2156 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa
//
LOCUS       DADVXO010000110         7974 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.114, whole genome shotgun sequence.
ACCESSION   DADVXO010000110 DADVXO010000000
VERSION     DADVXO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.114"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..582)
                     /locus_tag="KB950_002267"
     CDS             complement(<1..582)
                     /locus_tag="KB950_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBB6858449.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVL"
     gene            complement(584..1348)
                     /locus_tag="KB950_002268"
     CDS             complement(584..1348)
                     /locus_tag="KB950_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBB6858450.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1356..2033)
                     /locus_tag="KB950_002269"
     CDS             complement(1356..2033)
                     /locus_tag="KB950_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBB6858451.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2070..3455)
                     /locus_tag="KB950_002270"
     CDS             complement(2070..3455)
                     /locus_tag="KB950_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6858452.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3665..3749)
                     /gene="ffs"
                     /locus_tag="KB950_002271"
     ncRNA           complement(3665..3749)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KB950_002271"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3772..4287)
                     /gene="tadA"
                     /locus_tag="KB950_002272"
     CDS             complement(3772..4287)
                     /gene="tadA"
                     /locus_tag="KB950_002272"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBB6858453.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4393..5250
                     /locus_tag="KB950_002273"
     CDS             4393..5250
                     /locus_tag="KB950_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858454.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5331..5666)
                     /locus_tag="KB950_002274"
     CDS             complement(5331..5666)
                     /locus_tag="KB950_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858455.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5733..7103)
                     /locus_tag="KB950_002275"
     CDS             complement(5733..7103)
                     /locus_tag="KB950_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBB6858456.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7181..7717)
                     /locus_tag="KB950_002276"
     CDS             complement(7181..7717)
                     /locus_tag="KB950_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6858457.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2306 a         1691 c         1282 g         2695 t
ORIGIN      
        1 taatacaaaa aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat
       61 tggatcagta acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag
      121 gataaaaaag cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat
      181 gccgatttga aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat
      241 aaacaaaata ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat
      301 tttcacttca taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag
      361 gatggttttg aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt
      421 ctcttgatct aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag
      481 tggtcgaaag ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa
      541 caatccagca tattccatcc actgccatct cgaatttttc atattagaag ctccattcat
      601 ccagtgattg tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg
      661 taaaacctga taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg
      721 tttcatagtt atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg
      781 ctttatccat aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg
      841 tctctacaaa agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat
      901 tcttatctgg gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa
      961 ctactttttc ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt
     1021 cttcccatgt aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat
     1081 agactttttt cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt
     1141 agactgtctc tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt
     1201 ctggtgactt agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt
     1261 cttccacaaa tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta
     1321 aatcaattaa atatccttta tagtccattc atccactact cttccctttt tctgatcgta
     1381 aatgctgttt ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag
     1441 gtttttgctg actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact
     1501 ggtccttttt tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga
     1561 gttgtctttt cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg
     1621 caatattcgt aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga
     1681 ttatccgctt ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata
     1741 taatcatatc gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct
     1801 tcacgataat cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct
     1861 aacagtgtca ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc
     1921 aaagcagctg tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta
     1981 gcaatttctg ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca
     2041 ccacctatct tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa
     2101 tctcctacca cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca
     2161 atcgttggaa aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta
     2221 ttcagatcga ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt
     2281 ccattataga ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag
     2341 ttacgtaaaa agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca
     2401 cccaatgtga catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga
     2461 ttgacccgcc cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc
     2521 gtctttcctc gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag
     2581 atatctagac tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc
     2641 ccacgttcta gacaactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg
     2701 gtagggatgg ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg
     2761 acttcaccga catactgccc gtactttcca gcggctgcta tttggacatc attgattacg
     2821 tgaccttctc tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt
     2881 tcttttgcaa tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc
     2941 acttgtgaac gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc
     3001 cagccattcg gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc
     3061 gtctggtgtt tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta
     3121 tttttatcga ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc
     3181 aactcttctt tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca
     3241 acttcattca tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa
     3301 cgatccacaa aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt
     3361 tctcgtttac gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc
     3421 agatcattcg tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt
     3481 tatcatacca taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct
     3541 gccttgaagc aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg
     3601 aacattcatt tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt
     3661 ttcattataa tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca
     3721 cgcttcacaa gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc
     3781 attcgttgac cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc
     3841 agtcaaaatc atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt
     3901 aaaccgctcg tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata
     3961 cgcgccgaaa taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca
     4021 aggctctaag gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca
     4081 ggcttccttg attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag
     4141 gttatatcct cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc
     4201 atgaagcatt tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc
     4261 tactgcatca agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt
     4321 actttagcat atgataaaaa ggctgtaact aacttttttt ctatggtaaa ataaatacaa
     4381 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4441 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4501 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4561 atcaacgatc aaaaaaaaca cccatggtat cactatcttt ttaaagatgc ttcttgtgtt
     4621 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4681 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4741 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4801 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4861 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4921 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     4981 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5041 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5101 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5161 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5221 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5281 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5341 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5401 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5461 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5521 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5581 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5641 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5701 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5761 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5821 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5881 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     5941 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6001 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6061 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6121 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6181 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6241 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6301 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6361 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6421 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6481 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6541 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6601 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6661 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6721 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6781 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6841 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6901 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     6961 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7021 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7081 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7141 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7201 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7261 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7321 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7381 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7441 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7501 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7561 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7621 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7681 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7741 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7801 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7861 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7921 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccac
//
LOCUS       DADVXO010000111         7853 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.115, whole genome shotgun sequence.
ACCESSION   DADVXO010000111 DADVXO010000000
VERSION     DADVXO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.115"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            115..1881
                     /locus_tag="KB950_002277"
     CDS             115..1881
                     /locus_tag="KB950_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBB6858458.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            1983..3242
                     /locus_tag="KB950_002278"
     CDS             1983..3242
                     /locus_tag="KB950_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6858459.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3389..4690
                     /locus_tag="KB950_002279"
     CDS             3389..4690
                     /locus_tag="KB950_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858460.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4693..5544
                     /locus_tag="KB950_002280"
     CDS             4693..5544
                     /locus_tag="KB950_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858461.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5557..6372
                     /locus_tag="KB950_002281"
     CDS             5557..6372
                     /locus_tag="KB950_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBB6858462.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6523..7689
                     /locus_tag="KB950_002282"
     CDS             6523..7689
                     /locus_tag="KB950_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6858463.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2551 a         1360 c         1570 g         2372 t
ORIGIN      
        1 aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc gttgtctttt
       61 gcttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa atcaatggat
      121 acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac gaatacaaga
      181 tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt gctatgtgga
      241 gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt
      301 ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac aagtcgctta
      361 cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg tgatcaagga
      421 atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg aataccttat
      481 tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt atggtatcaa
      541 atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca cactttacct
      601 tggggaagta aagatccaga ccgagaagac tttttcgggg gagatttgca aggtgtgatg
      661 gatcatttgg attacttgac ggaccttggc atcaatggca tctatttttg tcccatattt
      721 aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga tcccgctttt
      781 ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg gatacgtatc
      841 atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca agacgttctc
      901 aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt cccagtagat
      961 tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga tacttttgct
     1021 tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa atatttgctg
     1081 gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct tgatgttgct
     1141 aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga aatcaaacca
     1201 gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca aggggatgaa
     1261 ttccatgcag tgatgaatta tgcatttaca gattcgatta aagattattt tgcaaaaaag
     1321 aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct ttacagagat
     1381 caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag gatcttgacc
     1441 ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa
     1501 gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga tgacccggat
     1561 tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt cggttttata
     1621 aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg ttctactcag
     1681 tggctaatag taaatgatcg tgaagataag ctctatttca cacggaaact agagggacag
     1741 atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag
     1801 gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat acgacaatac
     1861 ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg
     1921 aagatttgtg cccattataa taagggtgta acagcacgtt gaatacaaag gaggaaatga
     1981 agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta ggaatcttag
     2041 gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc gatgaaggaa
     2101 aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa ataaaaaaca
     2161 ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg ttcgatcagt
     2221 tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg atggcagcat
     2281 atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg
     2341 aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa atctacggag
     2401 aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc gatacagcac
     2461 catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct tttgagtcag
     2521 aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat tcctatggtt
     2581 taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct tcagatatcg
     2641 gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg
     2701 tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc cagcaattca
     2761 tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca tacaaagaaa
     2821 acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag ccttatcaac
     2881 cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa gacctctctc
     2941 aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa
     3001 atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac gatgaattaa
     3061 caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg
     3121 gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac
     3181 caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa caaaaatact
     3241 gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca gataaaagtg
     3301 gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg
     3361 gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca atatcaacga
     3421 aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa atggccaagc
     3481 ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga tgcttctggg
     3541 cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg aagatcattc
     3601 attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt tcttgatttt
     3661 ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa
     3721 aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt ttccttatct
     3781 attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag tcctagttac
     3841 attgttcact gcatttacta attatgactt caatcatatt ccaccagcca gtttaattga
     3901 ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc gtaatacttt
     3961 ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt tgcaaatcac
     4021 ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca aacgtatttt
     4081 cggtatcatt ttcttattac catgggcggt cccagctttt attactatca tgagtttttc
     4141 gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt tcataaacca
     4201 gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt
     4261 agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga tggttacagg
     4321 aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg gagcaaatgc
     4381 atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt
     4441 tattacacag tatacgggga atttcaataa tttctccatg atctatctgt ttaacaatgg
     4501 cggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt cctggatcta
     4561 taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca cgttgattat
     4621 ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg cgttcaatat
     4681 gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg actttctcac
     4741 ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc gcttttgatc
     4801 acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga tctttcaagt
     4861 gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg gacttggtat
     4921 aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt agtaactttg
     4981 gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagtct gatgttcttt
     5041 ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt catggctttc
     5101 ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt
     5161 ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa
     5221 tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt
     5281 gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc
     5341 atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa
     5401 acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg
     5461 attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg
     5521 acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat
     5581 ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct
     5641 aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt
     5701 tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc
     5761 gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca
     5821 tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa
     5881 caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa
     5941 aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat
     6001 tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat
     6061 cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg
     6121 agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca
     6181 tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc
     6241 atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa
     6301 acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag
     6361 cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat
     6421 aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt
     6481 tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta
     6541 tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt
     6601 acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa
     6661 gaaatcccaa tgaaagagat tacaagtaac gcatctttgg cactaggaga agcctatatg
     6721 gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa
     6781 gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat
     6841 acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat
     6901 aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat
     6961 acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa
     7021 gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc
     7081 aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt
     7141 accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac
     7201 cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta
     7261 ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga
     7321 acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat
     7381 aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca
     7441 aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta
     7501 gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat
     7561 tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt
     7621 aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat
     7681 cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat
     7741 gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtttc
     7801 tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca tga
//
LOCUS       DADVXO010000112         7813 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.116, whole genome shotgun sequence.
ACCESSION   DADVXO010000112 DADVXO010000000
VERSION     DADVXO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.116"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(33..1385)
                     /gene="celB"
                     /locus_tag="KB950_002283"
     CDS             complement(33..1385)
                     /gene="celB"
                     /locus_tag="KB950_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBB6858464.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1385..1903)
                     /locus_tag="KB950_002284"
     CDS             complement(1385..1903)
                     /locus_tag="KB950_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858465.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1919..2245)
                     /locus_tag="KB950_002285"
     CDS             complement(1919..2245)
                     /locus_tag="KB950_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBB6858466.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2258..4237)
                     /locus_tag="KB950_002286"
     CDS             complement(2258..4237)
                     /locus_tag="KB950_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6858467.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4258..4572)
                     /locus_tag="KB950_002287"
     CDS             complement(4258..4572)
                     /locus_tag="KB950_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBB6858468.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4740..5033
                     /locus_tag="KB950_002288"
     CDS             4740..5033
                     /locus_tag="KB950_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBB6858469.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5111..6223)
                     /locus_tag="KB950_002289"
     CDS             complement(5111..6223)
                     /locus_tag="KB950_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6858470.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6376..6600
                     /locus_tag="KB950_002290"
     CDS             6376..6600
                     /locus_tag="KB950_002290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858471.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            6610..6789
                     /locus_tag="KB950_002291"
     CDS             6610..6789
                     /locus_tag="KB950_002291"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858472.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6983..7153
                     /locus_tag="KB950_002292"
     CDS             6983..7153
                     /locus_tag="KB950_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6858473.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7577..>7813)
                     /locus_tag="KB950_002293"
     CDS             complement(7577..>7813)
                     /locus_tag="KB950_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6858474.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2496 a         1554 c         1262 g         2501 t
ORIGIN      
        1 attttttgtc acagttcctt ttttcttaga aatcatttta acttttcact agctaattct
       61 gctgcctttt ctctttcttc atttaataat ttattatcat agaatttgat aaatgggaac
      121 cagataatga atgcgatgat tgcacaaatg actgctaaca cagcagcttt ccaatcacca
      181 ccactaccaa caaatgctcc gatcccgact ggtgaaggcc acggcatctg tgcaagcatc
      241 ggtttgacga tttccagccg aatggcaaaa taagcgagtg tagcagaagc catcggagct
      301 aagaaaaacg gaaccgctaa atagggattg tagacgattg gcatcccgaa aatgattggt
      361 tcattgatat tgaataatcc tggtacaagt gaggcttttc ctaacacttt cagctgatca
      421 gatttcgcga ggaatgcaat aaagatgatc aatcctaaag tcgcacctga tccaccaacg
      481 gttacataag aattgttgaa ttctcctgcc aacggataat ttgcgccttg tgcgttagct
      541 gccatattag ctaatacgat cggggtcaag aaagaagtga tgatattcgc tccatggatc
      601 cctacgatcc acaatgcatg gatcaagaaa taaatcacca tgacaccaag ccatgtattc
      661 gtcaactgcg taacaaaccc aaatgggata gcaatcattt tgaagatatc cgttcctagt
      721 gcaactaata atccattgat gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga
      781 gcagtgaaag aacgagaaac tcctggcgga acaacatctg gcatttttat gacccaattt
      841 ctcttgacac acatacaata caattcgacc gcaataatcg acataataat agctgtaaag
      901 attcctgaag ttcctaaacg ttcgacaaaa gagcccatcc taataccatt tactacagtt
      961 tctgtctcat tgatagaagc aatcaagctg attttaccat cagtcatgat caattcaggg
     1021 atacacataa agaatgcaaa aacagatagt aatgcccccg tgagcggatt aacatttaat
     1081 ttttgttcat ctcgttcaat tgacgttaat tcataagcaa acacaatggc aaagtaaata
     1141 gataaaatcc ccatcgtcat tgtattgacg atcatgtaca agtcactaac tttgaaaaaa
     1201 gttgcattaa aaaatccttc taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa
     1261 aacatcgagc ctacaatcgt gaaaggaata gaagccatcc ccgctgccat gacagcccga
     1321 acgattttaa gttgcgcaat tttccccata ggtcccatca ggtatttctg caaaaaatca
     1381 aacattagtt attctccttt cctaaatata acgctttttc atattgttta atacggatat
     1441 actgttggtc ttggttgagt gaaattttct ttaaacgata ctgtataaat gcactggcaa
     1501 aaataccgct tacaacaaga atgaaaacaa ataactgtcc tccattgtta tttgccataa
     1561 aaggatacaa gaatgtaaat cctgaggtaa acaacactgg aatcactaat aaattaaaga
     1621 cactctggaa caaaaaatac cactttgtcc atggaacgtt cgtttggtgt tttcgataaa
     1681 gctttacttg ttctcctact gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac
     1741 ttcctaaact tttagctgat agaagcagaa tcatccattg catgttcatg aaaaagaaaa
     1801 aggcagtcag gtaacgtacg attagataac gattgaaata catgtttttc aaactaagtt
     1861 gctttttttc taattcgatt ttccttttta ctgtatcctg cacaatgatc cttcctttct
     1921 atttattttt ttgatacaaa taattcattt cctgtgcaac ttccaataat gtcatcgtcg
     1981 tcatcagatg atcttgtgca tggaccatga taatttccat ttctattttt gtcccgctgg
     2041 catattcttt cagaagttga gtttgtgact gatgcgcatg aagaatctct tcatttgctt
     2101 gtcgcaactt ctcttctact tcttcaaaca tctctttacg catcgagcga aatgcttcat
     2161 gtatcagtgt ccgtgcattt ccgctatgga aaatgatatc aaaagaagtc acttgtagtg
     2221 tttcactgtt tattttttca gccatctgtt ttcctcctta ttcgatcagt ttgataaatt
     2281 tactgctgaa attctcaaaa ataggttcag ctaagatttc ctgctgtact tttagctggt
     2341 caactaacct aacgattgct ttagtcacga ccgtaattcc ttcattttcg acataagaag
     2401 gcgaaactaa aaaaacaaaa tgaatttctt gatattgatc ccactgcatc ccagaaggaa
     2461 tcaatgcaac ggctaccttt gtttcgattc caactggaac agcgggatgt gggactgcca
     2521 ctgtatcact gaatatgatt tgtcccatat tttcacgttg ctggatctgc tttcgcattt
     2581 ctcttatata tccttcttct tcatttatgc tcagcttttg cagcaaatca tgaagaattt
     2641 cttccttttt cggttcgtac gtataagtct tgaaaaattt ttcagacaac tgttcggtgt
     2701 aaatctgctt tttttgctgt agagaaaatg ctggtatcgg tctatctgag gaatgatagg
     2761 gtatagattc tccaactacc tttcgtattt tacgaacatc atcttcattc aaaaaaatac
     2821 tgacatgtaa aactggaatt ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca
     2881 ggtcgattcc ttttaaagtt gtttcattga tctcataata gccttttaca cttaccactg
     2941 taatattttt accgaattca ctgactacgc gattctttaa tagttgggca ctgccgtaac
     3001 ccgttgcaca gataatcaat gcatgtagtt tatgcgcagc tctttctttt tccaatgccg
     3061 ccatgagatg caaagccaaa taggcccatt catcttcatt gatggtatag tttttcaaag
     3121 ccggcatgtt gctgaaatgc ctttttactg cgtcaaagac tttgggattt tctttcttta
     3181 tctcctcagt caatgggttc tcaatgcgaa tacctcggct caatcgaatc atcatcggtg
     3241 acaaatgatc gagtagtcca ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt
     3301 cctgagacaa tcttttcaga actttcgcta attcctttga tagttcctga tcattttgat
     3361 tttctttgtg attcgactta gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg
     3421 gaaacgatac acctgcgatt acctcaatac gttgaacgat ccgctgcgcc acttgatact
     3481 ctactcgctt agaaacttct tgttcgatgc ctaattcctt gatttcaaac ccttctcgga
     3541 gccgtttgat ccccaaagcc aagtgtaaag tcagattctg tatgataaaa tccgaaactt
     3601 ttaacttagc ttctcttgtt tcatcgagaa tgataatgac tagctcttca aaactgatgt
     3661 ctgggaaaaa acggctttgc cctaaatatt ctttcaatga atttccataa tggtttccaa
     3721 aaaaagtatc catgataaaa tgccgtcgat ttctttcttc cccttcgatc catgtccctt
     3781 gaccatgctt agaaacgatt tttagtgaat atggaatcaa tcgctccttg atttcttgca
     3841 tgtcctttgt caaactggaa cgactgatat aaagctcttc tgccaaatca tccataaaaa
     3901 gaatggcatc ttccaaaagc aattggttca atatatactt ttgtcgatct tcggactcat
     3961 aaaatccttg cggttctcta gcatatccag gtactatttc ttgccgactc aaaaacaagt
     4021 caaatgtcag tctttgtaaa atatgcagtt ggtagccata acctggttta gcaatgatct
     4081 tacctccgtt tttttctact aactctttga tccgctgcaa gtaattacgg attgtccgat
     4141 cagataataa caattcagaa gctaaacgtt gactggtgac atactccttc ttgtgatcaa
     4201 tcaaatatct caacagcaat ttttccttct gtttcattat catccactcc tattccatca
     4261 tatgttctct tcgatcacct ctgctaactt ctggatgcct gtagggattg ggacatacgc
     4321 ttggaaaggg atactaacaa caggctttcc aacagattct cctaattttt tgaatttatc
     4381 caaaaacatc gtcgtttgcg ggctgatcag aaataaatca aaatcactat tcttaatcat
     4441 attatcgcct tctgtagctg aaactgcatc tacgagtacc tcttctcttt tttgcttaaa
     4501 gaaatccgtt gtctttgagg ccatcatcga ggatgacatt cctgccgcac aaataatcaa
     4561 tgcttttttc ataaatcaat cactcctttt atttttataa accaagcata tcttgtaagc
     4621 gttttctatt caaaatatat atttccgtag cattgcggaa aaaatgtgta tcatatcttg
     4681 aataataatt tctggcaccg tgatatacta gtgatagaat tttttcagga ggggatttag
     4741 tgagtatcac agtgaatatt ttgtatacaa gtacaaatgg acaagccaga aaatttgcag
     4801 aagaaatggt cgaaagaaac attgtcaaag cgattcgtgc agaagaagga aacgagaaat
     4861 atgactatta ctttccacta gaagatccag aatctctttt actgatcgac cgttggaaag
     4921 atgaagaagc aatcgaaaag catcacaaat ccgaaatgat ggctcaaata aacgaactcc
     4981 gtaaaaagta taaattaaaa atgaaagtcg aacgtttcac tgatttaccg taaaaagata
     5041 cacatggaat gatagcaatt tatcatccat gtgtatcttc actttttcct tattttttat
     5101 tcgatctcat ctaatgattc aaaccgtgtt tttctcttat ttttcggata agtatttcag
     5161 tcaccccaat atacatgatt cctaaacata ccccaaggat attctcaaaa atgcggatcg
     5221 ttactgctcc tggaaccccg aataaactgg cagtgattgc caaagcacca aaagaattaa
     5281 aaactgtttt atatctatag gtagaacaaa cacctaaagc tagaccacct aatataccga
     5341 cccagttaaa aggaataaat tggctgatac caataaataa aacacatcca atcaatgagc
     5401 caatgattcg gtctactgct cgttctttga aaccaatcga catcaatcca tagctagata
     5461 caatagaaga aaatgcaaac cctgcccaca tcaatcgctg aaaagggaca tactctcctg
     5521 caacgatcaa aagactaatg ccaaatgcat agatgagcat ccaaattttt tcctgcgaat
     5581 acatgccttt caaaaaaatc tctttaaaca agcttttgtc tttgttcttt tctcgatgtt
     5641 ttctgtaaag gagaactgaa aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg
     5701 taaagaaatt cttgttcaac gaatctttag gtaacgaata aacaataaag agatatgaaa
     5761 agccgtacaa agaagcattc cccattttgg gatcactagc ggttgccatt aatatgctgc
     5821 ttagtaaaat aaaatgaatc aacatgccca taactgacca tttgatcagc tggacatacg
     5881 gtgctactaa tagtgagaac atgacgattg ctaaacctat gatcgaatgg gaaatcttat
     5941 aaccaaagtc aacaaaacga atactcatca acatacagaa taaaaccaca gcgtatggtt
     6001 tatgcttcga ttcaaataat gttgtaatta taatgataaa acaaatggca aacaatacga
     6061 tgaataatga tcgtaaaaac aatgaacaaa tatacatgca cttttctttt ttagaagctg
     6121 cttctcgtat tttttgtttt aaaataaacg gatctagttg tagagactga taaaaatcca
     6181 cctgttgtcg tcctttcttt ttacttttat tttttacagt catctatgac aggttgtaaa
     6241 tactatggaa catcatatgg cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt
     6301 tttataaaaa aacatatcgt tttcttgtat gattaaatca ccacaaaata aacaactgag
     6361 agaacgatat gctttatgaa tggttctatc aaaaaaattc tgagatcaat agacaaaaat
     6421 ttgatgatta cagaggtctc ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct
     6481 gcagttctct tccctgctcc atgtgtctgt agaaattgtg gttctgctgt ggtagatgga
     6541 aataggaaat taatggtggt gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa
     6601 tataatcata tgccaatggt tatacgccta aaaaagcagc gctatacctg taaaaactgt
     6661 cgaagccatt ggaacgctca aagttatttt atccgtccaa ggcactcgat ttcaaatcat
     6721 gtcagacata aaatcacttc tttactgact gaaaaagtgt cgttattttt tatttcgaaa
     6781 agttgttagg tatctttgac aaccgttatt cgcacattga aagagttgag aagctattta
     6841 ccaaagtctt ccaaaagaat tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg
     6901 tttccgttga ggataaactg agttttattt ccgcggatgg cgagacagaa aaattaatcg
     6961 agaaaattta cctacacaaa aaattcctcg attaacaaac tatttcctta gctgcactaa
     7021 tcctaaagaa atagaattct tagttacaga catgaacgcc acctactttc agctcaccaa
     7081 acatgttcta ccaaatgcga aattggtgat tgatcgatta cttggttttt ccacgccttt
     7141 caaggaggcg taacctttat tcacgagttg gttgaagctt tccgagacaa aagacccgac
     7201 ttgtttttct ccttattgac aaagagccat taaaactaga gaaaaaatta caactgatcg
     7261 ctgattattt gtagaaagaa aatcaaagta ttacccaata aacttgagtc attaggcaaa
     7321 aaacactgcg aaactcaaga atgattttcg cagtgttttt gtatattttt tattaactag
     7381 tattactaca aaggtttatt tttcacttct caacgcagca caaaattgtc tttacttatt
     7441 tactggttgg gttcaacctt ctctactccg atactaatat ttgtttgtat accccttcta
     7501 aaatcggtag tttccattca attgatgggt actagttgat tttaaaaact atgcttattc
     7561 aattttttat tttagctcat atatttcgtt tctttctaaa ttttatactg atgaaaacaa
     7621 gtagcacacc caatattgac aatataaact ggctaatgat ggtttctcca gtttttggaa
     7681 gatgcttatt tccactgctt gttggattgt ttcctcttat atctgagcta tcagcaactt
     7741 ttctattctc ttttgttaaa aagatcgcca cttcttgttc tttttcgatc ttgaaagtta
     7801 ctggtgtcgc atc
//
LOCUS       DADVXO010000113         7729 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.117, whole genome shotgun sequence.
ACCESSION   DADVXO010000113 DADVXO010000000
VERSION     DADVXO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.117"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            17..2344
                     /locus_tag="KB950_002294"
     CDS             17..2344
                     /locus_tag="KB950_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6858475.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2470..2799
                     /locus_tag="KB950_002295"
     CDS             2470..2799
                     /locus_tag="KB950_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBB6858476.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2839..3843
                     /locus_tag="KB950_002296"
     CDS             2839..3843
                     /locus_tag="KB950_002296"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBB6858477.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3840..4286
                     /locus_tag="KB950_002297"
     CDS             3840..4286
                     /locus_tag="KB950_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBB6858478.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="KB950_002298"
     CDS             4436..5251
                     /locus_tag="KB950_002298"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6858479.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="KB950_002299"
     CDS             5457..6629
                     /locus_tag="KB950_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6858480.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7324)
                     /locus_tag="KB950_002300"
     CDS             complement(6839..7324)
                     /locus_tag="KB950_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858481.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQEAI
                     AQQQAEQARQQSAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     S"
     gene            complement(7321..7515)
                     /locus_tag="KB950_002301"
     CDS             complement(7321..7515)
                     /locus_tag="KB950_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858482.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2590 a         1458 c         1540 g         2141 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt
     7081 ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt
     7141 cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag
     7201 caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag
     7261 ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg
     7321 tcactttttt tctagtgcag ctttttccgc tttttgtttc tcttcttttt tcttttcaat
     7381 ttgttcttgt tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga
     7441 agcctgatct gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa
     7501 acttcctgcg accaaaaaca atgcaactaa ttttttcgct aaatttcctc cttaagtaaa
     7561 aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt
     7621 gttatgaata gtgattgagg aaacttaatc taatcccgtg ttgatacacg ggattttttg
     7681 ctatagttct gttattaagt atgcaataat aaaatatgta cgttatttt
//
LOCUS       DADVXO010000114         7723 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.118, whole genome shotgun sequence.
ACCESSION   DADVXO010000114 DADVXO010000000
VERSION     DADVXO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.118"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..107
                     /locus_tag="KB950_002302"
     CDS             <1..107
                     /locus_tag="KB950_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HBB6858483.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="KB950_002303"
     CDS             complement(176..904)
                     /locus_tag="KB950_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858484.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="KB950_002304"
     CDS             complement(917..2197)
                     /locus_tag="KB950_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6858485.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="KB950_002305"
     CDS             complement(2338..2940)
                     /locus_tag="KB950_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBB6858486.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="KB950_002306"
     CDS             3185..3391
                     /locus_tag="KB950_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858487.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="KB950_002307"
     CDS             3703..4422
                     /locus_tag="KB950_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6858488.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="KB950_002308"
     CDS             complement(4503..5186)
                     /locus_tag="KB950_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBB6858489.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="KB950_002309"
     CDS             5405..7591
                     /locus_tag="KB950_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBB6858490.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2771 a         1301 c         1456 g         2195 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc gga
//
LOCUS       DADVXO010000115         7661 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.119, whole genome shotgun sequence.
ACCESSION   DADVXO010000115 DADVXO010000000
VERSION     DADVXO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.119"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            156..3383
                     /gene="fss3"
                     /locus_tag="KB950_002310"
     CDS             156..3383
                     /gene="fss3"
                     /locus_tag="KB950_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBB6858491.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3496..3810
                     /locus_tag="KB950_002311"
     CDS             3496..3810
                     /locus_tag="KB950_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6858492.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3823..4197
                     /locus_tag="KB950_002312"
     CDS             3823..4197
                     /locus_tag="KB950_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6858493.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4207..4542
                     /locus_tag="KB950_002313"
     CDS             4207..4542
                     /locus_tag="KB950_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBB6858494.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4625..5965
                     /locus_tag="KB950_002314"
     CDS             4625..5965
                     /locus_tag="KB950_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBB6858495.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6043..6717
                     /locus_tag="KB950_002315"
     CDS             6043..6717
                     /locus_tag="KB950_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6858496.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6710..6874
                     /locus_tag="KB950_002316"
     CDS             6710..6874
                     /locus_tag="KB950_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858497.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6950..7384
                     /gene="dcm"
                     /locus_tag="KB950_002317"
     CDS             6950..7384
                     /gene="dcm"
                     /locus_tag="KB950_002317"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBB6858498.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7385..>7661
                     /locus_tag="KB950_002318"
     CDS             7385..>7661
                     /locus_tag="KB950_002318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBB6858499.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLL"
BASE COUNT         2547 a         1294 c         1700 g         2120 t
ORIGIN      
        1 ggcagtatat ttcgtatttt agattaaatt tttaggctca ttcattctca aatccattct
       61 attcagtcaa taaaaatttt aaaaagactt tataaacaaa gatgttcaag cgagcatctt
      121 atttttttgt gcttaatttg aaaggagaac atttcatgta taaaaaacaa aaaaagaaat
      181 tatgtgtatt aactattttg agtttggctg ttcaagcaat tgttggagca attgctccta
      241 cgattacttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa ctagatttag
      301 cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca gaagtcactg
      361 ttccgcatta tgcagtgtac aacggcttta aacaagatat tttctgtatt gaaccaggag
      421 tcccgattta caatgagttc actcctggct atgagaaaaa cccgttacca gatatgtccg
      481 ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca gatactcata
      541 ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac tcaataaaac
      601 gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaataaat caagccattg
      661 acgattatca gaagaaacca accttccata atagtacggc taaaacagta ttagggcaat
      721 cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag gttgtggaaa
      781 atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt gataatcacc ccaaatgcca
      841 attcaaaaga aagtggtgtg ctgacgctta agaaatctgc tggtaccgga acacctgtcg
      901 cctataaaaa ggctggccaa cagactttaa tggccggggc gattgataaa cctaatacct
      961 acacaatcaa aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa gtcgataaag
     1021 aatcgggtga tgttgtacca ggaacggttt tccatttaga ctttggaaaa actttacctg
     1081 caaaagacgt gactactgac aaagaaggca ttgctacatt ggatgagatt cctcatggta
     1141 caaaagtaac cattaccgaa aaatcagtac cggcgcctta tacgattgat accaccccca
     1201 tgactaccac cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac gcacgggaaa
     1261 aagggcaaat cattctggat aaaagcggag ttgaaacagg gagtgatctt tggaatgaca
     1321 attattcctt agctggtaat acgtttgcca ttcgcaaaga cagtccaacc ggtgaaatcg
     1381 ttcaagaaat gaccacggat gaaaacggtc atgcggaaac accaaaagag attgctaatg
     1441 cgttggaatt gggaacctat tatgtgaccg aaactaaagc tagtcatggg ttcgtgaata
     1501 cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc cctgttacca
     1561 gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac cactttgacg aaagaagaca
     1621 aagatactgg ggataaagca caaggtaagg cagtctttga gggtgctgaa tacaccttgt
     1681 ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca gagttagcga
     1741 agggaacgaa aggttctgat gaaacggtca ctttgacttt agacgaaaag aaccaagttg
     1801 ccgttaaaca tttagctatc aacgagtatt attggcaaga aacaaaagcg cccgaaggat
     1861 atagccttga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa
     1921 atgccgtgat tacccgaaat gttacggcaa aagaacaagt gattcgcttt ggctttgatt
     1981 tctttaaatt tgctggatca gctgcgggca ctaccgaaac tggcttcaat gatctgacct
     2041 ttaaagtgtc gccattggaa gggaccaatg aaatcacagg tgccgaagat gaagcgacca
     2101 cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat cttccttatg
     2161 gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt acgccattag
     2221 aaatccgttc tacttttaag gaaaacaaag aagactttgt gaagagtgag tatgtcttta
     2281 ccattaccga acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct tacgagaagc
     2341 tgacgaacaa agcattttct gttagtttga accgtttaat gctctatgat ttgcctgagg
     2401 aagaagatag tttaacttct cttgcgactt ggaaagacgg aaacaaagaa ttaacttctc
     2461 tggattctac cgagctagtt gataaattga gttacaatct acatgaaatc aaagaagact
     2521 ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa gaaaaagatg
     2581 aaaaagccaa accagtggtg attgcagaaa caagcgcaac gttggcgaac aaagagaaaa
     2641 caggaacttg gaaaattcag cataaattaa ccgccgaaca agttttaaat aaaactattg
     2701 tcttattcaa ctatgtgtat gaaaataagg aagcttttga agcaggcgat aagccagtag
     2761 cgaaggatgt tagcttgaat aaccaagccc aaactgtcag ttgtacagtg gaacaccatg
     2821 tttctatcca aacaaaggcc cacctagaaa acggttctca gacctttact catggtgatg
     2881 tggtggatat gtttgatgat gtgtcgatta ctcatgatgt actggatggc tcaaaagaag
     2941 ctttcgaaac aattctgtat gctttattac cggatggtac aaacaaagaa atttggaaat
     3001 ctggcaagat tgattatgaa gtaaatgata aagaattcac caaaactgtg cttgcgaaaa
     3061 aagtagatac cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa atcaattacg
     3121 ataaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa tctcaaacct
     3181 taacaccaaa agaagttcca accatactaa gtacgccgaa acaaccggaa acaccaactg
     3241 ttccaagtgg ctctcaagaa tctagtccca cagtgaagac cttccctcaa accggggaga
     3301 aaaattccaa tgttttactg ttcattggtt ttactttgat ttttgcgacg gcgggctatt
     3361 atttctggaa tcgccggaac taaggtgatg agatgataca agtaaatcct aagaaccaac
     3421 gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa ctaacaatga
     3481 aaagaggaaa agaagatgga attgaaattt gtcgtgccag atatggctga aacctttgga
     3541 aagttaagtt atgccggtga aggagaaatt ataacagaag gatatggacg gaatactacg
     3601 gtgattggtc gcagttatca tttgtattcc agtaagcaac gggccgatga tattgaggtg
     3661 gtagtagctg cggaagccgg tgaaaaggat tttgatcaag accaaccgct aaaagccgtg
     3721 aatccccatt tggttgccaa aggctatgag attgagaatc gtgggtttac tgattacgtg
     3781 ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt
     3841 attgtcgtag attcaggttt aacatttggg aagttgcgtt tttccgcatt acgccgagaa
     3901 gtacggaagc aaaatgagga tggaacaatt ggtaatgagg taaaagaacg aacctataat
     3961 ttaaaatcct ctgcacaagg gcgaatgatt caagttagta tcccagctaa tgtaccctta
     4021 cgcgagtttg cttatgatgc agaggtagag ttggtcaatc caatcgtgga taccgttgcc
     4081 aattatgttt ttcgagaagg aactaccgtt aattggttca ttaaggcgga tgatttagtc
     4141 ttgaagcggc agccgaatca aggaaatcct acgaaccaaa atgaaagaaa gaaataggtg
     4201 aagattatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaata tggaaaaggc
     4261 gagctgactg aggaacaact cgttaaagca ttaacctctg atgaaaaaat gtttctgatt
     4321 atacatcaag gtttacttgg agaaaatagc gacagtgaag aagactctga tgtactagac
     4381 tggttaggag aagaagagtc tttctttata gttgtcgaag taaatgaaaa cttatgtcgg
     4441 caggcagaat ccattttgga agaaattggt gtggagatgc cggatgctat tgaaggcttt
     4501 ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt agcagtaaat gactagcata
     4561 actttttgaa attgcctaat gaaatcctat tgggcttttt cgattggagg tggaaaccat
     4621 caaaatgtac aaaggacatc gcatacgcgc aggagatcag cacttggttt atcactttgt
     4681 tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt ttcaagagtt
     4741 tagacaattt gatatttcta agctatcact ttctactatt gaaatcgttt ggtctataaa
     4801 agatttagta tgtctgcttg gaagtctcgc tttttcagga gctatgatat tgctttatat
     4861 tcactttttt cttgatcatt ggcgaagctt gtggcatcga caaaagctag ctcggatgat
     4921 tctagagaac cattggtatg aagtgaaaca aactcaaagt gaaggctttt tcaaagattt
     4981 gaatagtagc cgaaccagag agactatcag ttacttcccc aaaatctgtt atcgaatgaa
     5041 agatggatta ctgtctattc gcgttcaaat ttcacttggg aaataccaag atcagctttt
     5101 aaaattggaa aagaagttag aaagcggact gtattgtgag ttagtagaaa aggaactcaa
     5161 agactcctat gtggagtata ctttactgta tgatatgatc gccaatcgaa ttgggataga
     5221 cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa aatcaagtat gggcttatga
     5281 ttctttaccc catatgttaa tcgctggtgg cacgggtgga gggaagacct atttcctcct
     5341 caccattatc gaagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa
     5401 tgcagattta gctgatttag gtacggtgat gcctcatgtt tattcacaaa aggaagaaat
     5461 ttctgcttgt gtggaagatt tctatgaacg tatgatggct cgtagcaagg caatgaaaga
     5521 aatgtctaac tacaaaacag gagagaatta tgcctatctt ggacttccac ccaacttttt
     5581 aatctttgat gaatacgtgg cttatatgga gatgttaacg acgaaagaaa gtgcggtgat
     5641 tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct ttctgatttt
     5701 ggcttgccaa aggccggatg cgaaatattt aggagacgga atccgcgatc aattcaactt
     5761 tcgcgtggct ttgggtcgta tgagtgaact cggatattcc atgatgtttg gggaggtcga
     5821 aaaaaacttc tttatgaagc acatcaaagg tcgaggttat gtggatacag gtggcagtgt
     5881 gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttag attcaattag
     5941 taaattagaa aagatgaatg attgagtgat cttcaatact gactttatac ttcaagtaac
     6001 gtataatact tttatatagt aggaagaaag gggataatta atatggaaag taatgattct
     6061 tatttagagt atgcaaaagc aaataaggat aagtttattt cttatattat caaagggaaa
     6121 tcccccgaag ttggacgaga cgcagttttt atggcaggga gtcctggggc tggaaaatca
     6181 gaggttgcca ttggattagc tgagaattat gataaccatg tgattattga tgcggattac
     6241 tttcgcaccc agtttcctga ttataatgga aaaaactcta gtctctttca aaaagcatct
     6301 tcttggttgg tagagcaggc atttagatat gtcttagatc atggctattc ttttattctg
     6361 gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct cgcgggcgtt gaaaaaacat
     6421 tttcgagtga cgattttcta tgtttatcaa gaccctaaaa tcgcttggaa atttacacaa
     6481 gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg catttattaa cgcttttttc
     6541 aaatcaagag agaacattgc aaaagtaaag agtaaacatc cagatgtatt gcttcatatc
     6601 ataataaaag attatacgaa taatatttcc gaagttcatt atgctacgga taatctagaa
     6661 ttatttttac cactagggta tagtaggaaa gatttggagg ttcaattaca tgactgagaa
     6721 agaacaagta acgaaaattg taaagaaata caataagagc attgcggatt tatcggaaaa
     6781 tgctactgca aaagagttca aaacggtgat taaatatgta gccgatcagg ccaatgaaaa
     6841 acaacggaaa ttagttggat taaataaaaa atagtaggac aaattaataa tgtatgagcc
     6901 aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg aggtaatgta tgagattttt
     6961 agatttattc gccggtattg gtggctttcg tttaggtatg gaacaggcga gccatcattg
     7021 tatcggtttt tgtgaaattg ataaatttgc tagacgtagt tataaagcaa ttcatgatac
     7081 aagtaaggag gtggaaatgc atgacatcac aagtgtatca gatgagttta ttcaatccct
     7141 cggaccagtg gacatccttt gcggcggatt tccgtgccaa gctttttcaa ttgcgggaaa
     7201 gcggcaaaga ttttccgata ctcgaggtac tttattcttt gaaatcgctc ggttcaccac
     7261 tcttctccga cctaagcttt tattccttga gaacgtccga ggattgctca atcacgaagg
     7321 aggggctacg ttcgagacga tcctccgaac gctggatgga ttggggtatg acgtggagtg
     7381 gtaggtgctt aactcaaagg cctacattcc ccaaagccgg gagcggatat tccttatcgg
     7441 acattctcga gacgcatgta ccgaacaagt atttcctatc attggaccgt cttcaacatc
     7501 tgatcaaaac atcagaaatt tggtaaatat caatccttcc aatcgaggaa tgggtggaca
     7561 agtctatggt tcagatggcg tagcgccaac cttaacaggt gatgaaggaa tcaaaatcgc
     7621 actgccagca agggatggaa tctctgtagc aggactatta c
//
LOCUS       DADVXO010000116         7517 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.120, whole genome shotgun sequence.
ACCESSION   DADVXO010000116 DADVXO010000000
VERSION     DADVXO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.120"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="KB950_002319"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="KB950_002319"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBB6858500.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="KB950_002320"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="KB950_002320"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBB6858501.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="KB950_002321"
     CDS             complement(2298..2960)
                     /locus_tag="KB950_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6858502.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="KB950_002322"
     CDS             3144..4790
                     /locus_tag="KB950_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB6858503.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="KB950_002323"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="KB950_002323"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6858504.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7491)
                     /locus_tag="KB950_002324"
                     /pseudo
     CDS             complement(5971..7491)
                     /locus_tag="KB950_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331751.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2357 a         1373 c         1388 g         2399 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgag
//
LOCUS       DADVXO010000117         7514 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.121, whole genome shotgun sequence.
ACCESSION   DADVXO010000117 DADVXO010000000
VERSION     DADVXO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.121"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            16..2625
                     /gene="clpB"
                     /locus_tag="KB950_002325"
     CDS             16..2625
                     /gene="clpB"
                     /locus_tag="KB950_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBB6858505.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2776..3462)
                     /locus_tag="KB950_002326"
     CDS             complement(2776..3462)
                     /locus_tag="KB950_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858506.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3440..3934)
                     /locus_tag="KB950_002327"
     CDS             complement(3440..3934)
                     /locus_tag="KB950_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBB6858507.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3954..5174)
                     /locus_tag="KB950_002328"
     CDS             complement(3954..5174)
                     /locus_tag="KB950_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6858508.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5321..7156
                     /gene="lepA"
                     /locus_tag="KB950_002329"
     CDS             5321..7156
                     /gene="lepA"
                     /locus_tag="KB950_002329"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBB6858509.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7352..>7514
                     /locus_tag="KB950_002330"
     CDS             7352..>7514
                     /locus_tag="KB950_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6858510.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLS"
BASE COUNT         2489 a         1419 c         1532 g         2074 t
ORIGIN      
        1 ggagggtata cctgtatgaa tattgagaaa atgactacga cgcttcaaga agcgattgct
       61 gaagctcaac agattgcggt cactcgaaaa catcaagata tcgacattgc ccatgtatgg
      121 aaaatctttt tacaatctaa tcattttggt cgaaatttct atacggatgc cggattggat
      181 gtcgaatctt ttgaacatga aattgatcgt ctactggatg agtatccagt tgtttctggt
      241 ggaaacgtcc aatatggaca aaacctcagc cagaatttct ttcgattatt gaatgaagca
      301 gaccaaatac gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt tattttagcg
      361 ttgatgaaat taaaaaatta tccgttaact gtttatttga ataaaaacgg attatctgaa
      421 aaagaattgc gtaaaaacat cgaagagatg agaggaggag aaagagtgac ctcacaaaac
      481 caagaagaac aatacaaagc attagaaaaa tacggcgttg atttagtaca gcaagtcaaa
      541 agcggaaaaa tggatccaat catcggtcgt gatgaagaaa tccgtgatgt catccgtatt
      601 ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag aacccggtgt agggaaaacg
      661 gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga aaatttaaaa
      721 gataaaacga ttttttcatt agatatgggt gcattgatcg ctggagcaaa attccgtggt
      781 gaatttgaag aaagactaaa agcagtcttg aaagaagtca aaaaaagtga tggacgtatc
      841 ctcttgttca tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga aggaagtatg
      901 gatgccggaa atctgttgaa accaatgctt gctcgcggtg aattacattt gatcggtgcc
      961 acaactttag atgagtaccg tcaatatatg gaaaaagaca aagcgttgga acgtcgcttt
     1021 caaaaggtat tagtcaaaga accaacagtg gaagatacga tcagtattct gcgtggatta
     1081 aaagaacgtt ttgagatcca ccatggtgtt aacatccatg acaacgcatt agtagcagca
     1141 gctactttat ctgaccgtta tattactgat cggttcttac cggacaaagc aatcgatttg
     1201 atcgatgaag ccagtgcaac gattcgggta gaaatgaact ctatgccaac ggaattagat
     1261 caagttacta gacgtttgat gcagttggaa atcgaagaag ctgcactgaa aaaagaatca
     1321 gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga acttcgtgaa
     1381 gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa ttctgtttca
     1441 gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag aagatgcaga aaataactat
     1501 gatttagaac gcgctgctgt attgcgtcat ggtacgatac ctcaattaga aaaagaattg
     1561 aaggaattag aagctaaagc aaaagacagc gagattaaga tggttcaaga gtccgttacc
     1621 gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta tccccgtaac gaaacttgta
     1681 gaaggtgaac gagaaaaact aattaaattg aatgaaacac ttcataaacg agtgattggt
     1741 caagatgaag cagttgatgc ggtcagtgac gcggtaattc gttcacgggc tggattgcaa
     1801 gacccaaatc gtcctctagg ttctttcctt ttcctaggac caactggtgt cggaaaaacc
     1861 gaattagcga aagcgttagc tgagaacttg tttgactcag aagatcatat ggtacggatc
     1921 gatatgagtg aatatatgga aaaacatgct gtgtctcgtc tagtcggagc tcctccaggc
     1981 tatgtaggtt acgaagaagg tggtcaatta acggaagctg tcagacgaaa tccttataca
     2041 atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg tcttcaatat cttgttacaa
     2101 gtcctagatg acggacgctt gactgattca aaagggcgag tagtagattt caaaaatacg
     2161 gttctgatca tgaccagcaa tatcggttca cagttactgc tggaaggcgt gactgctgat
     2221 ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg aaacttcaaa
     2281 ccagaattct tgaaccgaat cgacgacacg attttattta caccattgag cctagacaac
     2341 gtgaaaggaa tcgttgacaa aatggtggcg caattggcac aacgtctgga acatcaagaa
     2401 atcctgctga caatcagtga tgaagccaaa acgtggattg ctgaaaatgc ttatgaacca
     2461 gcatatggtg caagaccatt gaaacgattt atcactaagg aagtagaaac accactagct
     2521 aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag taacgattac tttacttgat
     2581 ggacaacttc atttcaaaac agaagagcta gaagaaatcg tctaatcttc cattgagact
     2641 ataaaagaaa aaaggggtct gacacaacta gtgtcagacc tccttttttc aaataaacag
     2701 tgttccagaa gtagtttctt cgaaaaataa gccgaaattt cttgaagctg cccgcttctg
     2761 tcacaacctc ttttattagg tatttttcac tttttctaat ttataacttt caaagtaacg
     2821 ctcatctttt ttactttgag accatgcaaa caatcccatt aaggcagcac ctatcgtatt
     2881 tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca gtgtgccatc
     2941 agaagcagca aaacgttgaa gattcatgcc taagaactgg tcgcataaga attcccagaa
     3001 ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg aataagagga acaaccaagg
     3061 actggttttt gatatctcag catctttcat caagtaacca atcaatccat agccaagagc
     3121 tgtcaggacc attggactca ccgcatgtaa gattttgtcc caaaaagaga tgatactgat
     3181 caaatgcatc cctgtgccta aaaagacaga gatgaacaag aaaaaccagt aaaacaaaac
     3241 gattgctgct ggtattttaa gtttgaaaac tttcaaaatg acttgtggca tgaaaatcaa
     3301 tgcaatccct aataaagatt ccacgataaa tgtgattcct tgagaagtcg aatatttttg
     3361 tgtgataaat tcataaaaat tatagcagac aacgaataca ccaaaagcaa gtaaagctat
     3421 ttgcaccttt ttgatttctt taatcactga aagtccctcc atctaaaaat ctttttactt
     3481 ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt caatttgatc aaacctattg
     3541 tatataagtt caggtagtga aattgatttt cagctgtttc ttgtagtgca gcgatttttt
     3601 tcgggtgggt tccgccttgc tgtcgagtat cagtatataa gccgatcact gggatgcctt
     3661 tcgcataagc cacaccgatt tccgatgcta ccccggcatc gatcgtcagc ccatctagta
     3721 aagcaatcat taaatcacta gccaacactt tttctgtgtc agcaagagca atcattcgac
     3781 tgtctgcata agcagttttg tcattgattg cttcattttc ttgaggaaga tagatcgaaa
     3841 gctcagaaga gagctctctg atttctttaa ctaactgttg gttatataac aaatcacttt
     3901 tagcaaacat tggtgcggca aaataaattt tcatctaaac atcatccttt ttatcaaaaa
     3961 taatcgctaa agtaagcttc catgtttgga atgatacgct ctagtgactt cagtgtttct
     4021 tcatctgttt ctaaccgttc gattcttgcc aaataagaag cacggcgata attcatcatc
     4081 aagcaagtca atgtttggat atccagtcga acggattttc ctataggttc atttaagaca
     4141 cggacttgtc cctgttcatc ccatgttaaa gcgaagacac cattattcca aggagcgaca
     4201 ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa caaacggaaa ccgttgaagg
     4261 aattctttta catcaacgat ccgagccata aaatatggtt cgatctgctc tttgatctga
     4321 ctatcttcaa tcaaaaatgc taaaggctca tttttataaa tatctccatg tacccagtag
     4381 atcatggaaa aatgtgcgga aataaagttc cataagccat ttcgagcttc ttgatcaaga
     4441 tagaacattt cttttacgtg aaatatttct tcagctaccc agtagaacag aacacctctt
     4501 ggttcatggt tgctatcgta gtagacagcg gctgtgcgct catcttcatt ttcgaaacgc
     4561 caatactctt cccaatgaaa atcactgcgg aataaagcac catggttttg ttgggcaaat
     4621 ttgtcataaa cggaaaatac gtctggatga tcaactggta atcgctcaac cataccactt
     4681 actgggattt gtttaggtag ctgtgtatct ctgattttaa aggaaagttt atcagacatg
     4741 atttcccatc ctttacgccg atagtagggg atactgtaag gatacaaata agagatccat
     4801 tgcttattct tgcgcatatt atccaatgca acgataatta gatcttgcat caatccatgg
     4861 ttagcatatt ctggataagt tccaactcca gtcacaccac ccattttata acgtgcaccg
     4921 tgaatattga cttcacaagg atagatagcg atttgagaga tcagtttatt ttcgtggaac
     4981 catccgaaca ctttcgatac ttccaaaatc ggttttttgg aacggataaa agctcgttta
     5041 ttctcaaacc cgctttcctc gatgtctgct tcagtcactt ggaacacata ggaaagcaat
     5101 tcattgaatt gatcgatatg ttcttgagtg accggtttta attcaagatt ttctctaaaa
     5161 tctttttgat ccattttttc atctccattt attagcttga taacagtata gcaaaattct
     5221 acaaaaaaaa cttctatagc tgactaatta aaaactcttt aaattactgc ttttcgttgg
     5281 tataatggaa agtgctgatt atatgaaaag aggagacgct atggatataa agaaaatgaa
     5341 agaacgccaa gagcagattc gtaatttttc gatcatcgct catatcgacc atgggaaatc
     5401 tacattggcc gatcgtattt tagaaatgac gcacactgtg acgtctcgcg aaatgcaaga
     5461 tcagctgtta gactcgatgg atttggaacg tgaacgtgga atcacgatta aattaaatgc
     5521 tgtagaactc aattacacag cgaaaaatgg agaaacatat atctttcact tgattgacac
     5581 cccagggcac gtcgacttca cctatgaagt ctctcgaagc cttgctgcct gtgagggtgc
     5641 cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa acattggcca atgtttattt
     5701 agcgttagac aatgatttgg agatcctgcc agtcatcaat aaaatcgacc tacccgcagc
     5761 agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg ctagtgaagc
     5821 tgtattagcc agtgccaaat cagggattgg gattgaagat attctcgagc agatcgtgga
     5881 atatgttcct gcaccaagtg gagacttaga tgcaccgcta aaagctttga tctttgactc
     5941 tatttatgat agttaccgcg gtgttgttct aaatgtccgg atcacggacg gtatggtcaa
     6001 acccggagac aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta cagaggtcgg
     6061 ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg tcggttatat
     6121 cacagcaagt atcaaaactg tccaagatac acgagtaggg gatacagtaa cgttagcaga
     6181 caatcctgcc gctgaagctt taccaggtta ccgaaaaatg aatccgatgg tttattgtgg
     6241 tctttatcca attgatactt ctcgttacaa cgatttgcgg gaagctttag aaaaactgca
     6301 attgaatgat gctgctttac aattcgaacc agaaacctct caagcattag gttttgggtt
     6361 ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc caagaacgct tggaacggga
     6421 attcaatcta gaattgatca cgactgcacc atccgttatt taccatgtta ataaaacgga
     6481 tggtacgact gctacagtag ataacccggc agattttcct gaacctgtaa cgattcagga
     6541 tgtagaagaa ccattcgtca aagcacagat catggtccca aatgattttg tcggtgcagt
     6601 aatggaactt tctcaacgaa aacggggaga attcattacg atggattatt tagatgatta
     6661 ccgagtaaat gtcgtctata atattccgct ttctgaaatt gtctttgact ttttcgacaa
     6721 attgaaatca agcacgaaag gctatgcctc attggattat gaaatgtctg gttatcaaaa
     6781 aagcaagtta gtgaagatgg acatcttgct gaatggagaa aaagtcgatg cattaagctt
     6841 tatcgttcac cgagactttg cctatgaacg tggaaaagca atcgttgaaa aactgaaaaa
     6901 actaatccca cgccaacaat ttgaggtgcc aatccaagca gctattggac aaaaaatcgt
     6961 tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt atggtggaga
     7021 tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc ggatgaaaca
     7081 aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa tggatgagca
     7141 agatagtgtg aaataactta taatcaatcg aaaaacctat taaataaacc tttttgagta
     7201 gatgcaacac tgaggattat ctgtataaaa gattttaaac tgtcatttgg ttatcacggc
     7261 gatactaaaa ttaacaaaat tggaatattt ttcttgaaga tttttagctg tcagtatatg
     7321 atgaaagcat ctcataggag gtgctttttt catggttgat aattcaagtg gaagggttct
     7381 taaatctcta agaaaagaga aaaaactatc tcaaaaaaag ttagctgatt tagcaggtat
     7441 ttcacaaagt actttagtta aatatgaaaa agggtcaaga aaaatcccta aagacgtaga
     7501 taatactcta agca
//
LOCUS       DADVXO010000118         7284 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.122, whole genome shotgun sequence.
ACCESSION   DADVXO010000118 DADVXO010000000
VERSION     DADVXO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.122"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(205..1653)
                     /gene="cls"
                     /locus_tag="KB950_002331"
     CDS             complement(205..1653)
                     /gene="cls"
                     /locus_tag="KB950_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBB6858511.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1805..2698)
                     /locus_tag="KB950_002332"
     CDS             complement(1805..2698)
                     /locus_tag="KB950_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858512.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2682..3368)
                     /gene="ftsE"
                     /locus_tag="KB950_002333"
     CDS             complement(2682..3368)
                     /gene="ftsE"
                     /locus_tag="KB950_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBB6858513.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3473..4574)
                     /gene="prfB"
                     /locus_tag="KB950_002334"
     CDS             complement(join(3473..4501,4503..4574))
                     /gene="prfB"
                     /locus_tag="KB950_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBB6858514.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4700..7234)
                     /gene="secA"
                     /locus_tag="KB950_002335"
     CDS             complement(4700..7234)
                     /gene="secA"
                     /locus_tag="KB950_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBB6858515.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1935 a         1627 c         1277 g         2445 t
ORIGIN      
        1 tgctatactg gctttgaaat gtgtctcttt taatagcata tattagctta tttcttgctt
       61 tccccaaaaa acaagagata ccccaaagtt aatttcgctc atgcgagacg ctttctccat
      121 ttctgacgta ctctcagaaa tggccccgtt aaaaaaggac acgagatctc catctgttga
      181 tggttctcat gtccttttta tgttttaaag gagcggcgaa atcaatcttg ctaatccttc
      241 tttcatcttg atgattattc ctcggttttc atacatttct tttgtgagat gcgtagaaat
      301 tttcgagtct tcaaaaaaag cttttcttac acgtgaagcc attcgttcat cataaaccag
      361 tgtattgacc tcaaagtcca agcgaaatga acgaacatcg atatttgctg agccaactga
      421 aacaatgcca ccatcgataa tcatcgtctt tgcatgaata aatccattct catatgtttc
      481 gatccttgct ccatactcga tcagctctgc agcgaaagaa tacgttgccc agtaaacgag
      541 aagatgatct ggtttgttag gtatttggat atgaactttt acgcctgaaa gaagagccaa
      601 cttcagtgct tcatgaatcg atccatccgg tatgtaataa ggcgtttgga tcaatatctc
      661 ccgtttggct aacgaaatca ttttcaaata agtcattttg atttgttcat gttcggtatc
      721 tggtccgctt gtcacgattt gaacagctat atcaccagta gtgtgaatgg acggaaaaaa
      781 tttgggagaa tagcctactt cttttgtatg ctgagaattc cagtccatca aaaaacggtt
      841 ttgcaaggta tacaccgctt ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa
      901 tttctttacc aatcctagat attcatttcc aacattgaac ccacctgtat atccgatttc
      961 cccatcaata actacgattt tacgatgatt gcgataattg atccgaggat taatatacgg
     1021 aacaaataga ggaaagaaaa atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa
     1081 gctggaagaa acttgtgtgg aaccccatgc atcaagaagg actctgactt ttacccctcg
     1141 tcttgccgcc tctgtcaatg catctcgaac ttcttcacct aatgcatcac tgcgataaat
     1201 ataatactgg aagtggatat ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa
     1261 tttttcacga ccatctgtaa ataaagtgat ttcattttta gtcgtataga gcgtactttc
     1321 aaataccgtg agcatataga tcaattgttt cacatctact tgtccagtag gcggatgagg
     1381 ataaagccct cgcatcaatg cctgtttgtc ttcttcaatt tccaggttct taccgatttt
     1441 cccttggatc ttcaaatcaa agattttctc tttggaaatc cctcttccta aaaaaatata
     1501 taaaataaat cctacaactg gaatgaacat caaaacgagc agccatgccc atgtttgagc
     1561 agtttccttt ctttctcgaa aaacgatgat aaaggaaagc aaaatattca taatcaaaag
     1621 gatcgtaaaa aaattgtcaa cgataatttt cacggcttca cctcttcttc taccaactta
     1681 agaataccca agttgttttt taaagtaaag ggaaaaaagc cgcttccttc tattctccta
     1741 gccaataaaa aaagaaagct ggaacgaaac tgtccaactt tctcatttta tttatattgt
     1801 ggtattagaa tttcaagaaa cgtctcatag aaatgatcga gccaagtgaa ccaatgatca
     1861 caccaatgcc gaccattaaa agattgattt tccaaacgat attttgtgga tgaatcaatg
     1921 aataatggga acgtaataat gatggattga tcaattggaa aaaccatgca taaccaaatg
     1981 tgatgatcaa aattggaaga attgcaccga tcagaccgat ccagccacct tcaaggaaaa
     2041 acggccagcg aatatatcca ttttttgcac cgactaaacg catgatttga atttcacgct
     2101 gtctagacaa aatcgtgatc cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca
     2161 gcaatgccgc tgcaactaat ccccatgtcc gaactgcacc agcgatttgg aaaatccgat
     2221 ctgaggaagc tccaccgtaa tcagaacgag aaacattctt caaatctgct gcttcttctg
     2281 ctacttgttt tgtataagaa ggatcagtag cacttactac atacacatca tataacgggt
     2341 tattgtcacc ttcaaacaat ttccaggaat cacccatttc gttttggatt tttttcaatt
     2401 cctgttcttt gcttgaaaat tcaacttttt taacatgatc taagtcattc aattcttttt
     2461 ctagcttttt catctcttcg tcttttgtac cgatatctac gaatacagat acatcaacgt
     2521 ttccttctat atcctgagct aatttagttg cattcatgat aactgccatg aatacaccga
     2581 ctaaagtcaa cgtgatcgtt accgcactga ctgaagcaac tgtcatccag ccgttacgtt
     2641 tcaaactctt gatactttct aataaatggc ggaaaaaggt tctaatcatc gtatccatat
     2701 tccccttccg cttgatctcg aataatgcgg ccgttttcga tagcaattac tcggtgccga
     2761 atcgtattta cgatcgtact gttatgtgta gccatcacga cagtagttcc ttgggcattg
     2821 atccgatcta ataatttcat gatttcccat gagttttctg gatccagatt tcctgtcggt
     2881 tcatccgcga taagtacttt aggcgtattc acgattgccc gagcaataga gacacgttgc
     2941 tgttccccac cggataattc gctaggaaag actcgaactt tgtgtttcaa accaacaagg
     3001 tccagcactt ccattactcg ttttttgata tctcgaggtt ttctgccgat aacttgcata
     3061 gcgtacgcaa cattttcata tactgttttt cttggtaata atttatagtc ttggaacacc
     3121 acgccgattt ctctgcgtag ataaggaact tcgcggtttt tgattgctat tagatcatga
     3181 cctgcgacgt tcaaagttcc ttttgaagct ttttcttctc gatacatcaa tttgatgaat
     3241 gtagatttac cggctccaga cggaccaacc acatagacaa actctccttg ttcgatgaca
     3301 atcgagagat tgcggatagc ggtcgtacca tttgagtact tcttcattac atccttcatt
     3361 tcaatcatgg cgttctctcc atatcttttt cagtctttag ctattatagc atggcaatgt
     3421 ttcaacatgc ttgcaaaatc ttacaatttt atttcaattt aacaatgggt tcttattgta
     3481 gatgcatttt taaatagccg tcaataaata aatcaaggtc accgtccata actgcttgga
     3541 catttcctgt ctctacgttt gtccgatggt cttttaccat agaataggga tggaaaacat
     3601 aagaacggat ctgtgaaccc cagccgattt ctttttgttc tcctcgaagt gcagctgctt
     3661 cttgttcttt cttttccact tctaattgat aaagcttagc ttttaacatc cccatcgctt
     3721 gctcacggtt tttcaattgt gagcgttgtg cctgactagc tactactgtt cctgtaggca
     3781 agtgagtgat ccgtacagca gattctgttt tattgatgtg ctgaccacct gcaccacttg
     3841 ctcggtacgt atcgattttc aaatcatctg gattgatttc tatatcgatc gtttcatcaa
     3901 gctctggcat cacatctacc gaacaaaaag aagtatgacg tcgtttcgct gagtcaaaag
     3961 gcgaaatccg tacaaggcga tgaacacctt tttctgattt tagataacca aaagcgttat
     4021 gtcctttaat cagcagggtc acactcttga tccctgcttc atcacctgct tgataatcta
     4081 gtgtttctac ttgatagcca tgctgctctg cccaacgtgt atacatacgt aaaagcatgc
     4141 tgccccagtc ttgggactcc gttccgccag cccctggatg caattcgacg atcgcattgt
     4201 ttcgatcata aggtccgtct aacagcatag ataattcata cgtcttcatt ttttcatcta
     4261 atgcattgat acgtgtttct gcttcttctt gcattgctgc atcaggttct tcttgcagca
     4321 tctccaccat caattcgatt tcttctagtt cttcggcaag agaatggaat tggtcatagg
     4381 tttctttatt agcgttattt tcgctgatca ctttttgtgc ggcttcgctg tcatcccaaa
     4441 aaccaggttc agccattttt gcttctgctt ccgcgatgtc ttcttccagc tgatctaagt
     4501 caaagagacc tcctgaaact tgtgaccttt tcctgcattt ctgtcaggag atttcgaatt
     4561 tcggatattt ccatgttttt tccttctttc actatacttt tttcattata tctagtctaa
     4621 cagaatcagc aaataacaga aagaaggtga gacgaattct taaaaaaaga atttgtctca
     4681 ccaatcctgc tgtcagaaat tagtttgcat tttttccatg acagttctta tattttttcc
     4741 cgcttccaca aggacaagga tcatttcttc cgaccttatt cacatggatt ggctgttttt
     4801 tcgcgctagt attactattt tctttggcat ctccgtcttc tgagtgagaa gctgttcctt
     4861 gagaaacctg ttcacgctgt acgttttgac ggatctcagc tttcataaag attcgagtga
     4921 cttcatactc gatggcgcct accatatctt caaacatctt atagccttct gtttgatatt
     4981 cgactaatgg attgttctgt ccataagcac gtaagccgat tgactgacgt aattgatcca
     5041 ttgcatcaat atggtctgtc catttagagt ccacaacacg caagatcacg actttttcaa
     5101 attccaacag ttgctctgga ccatttagtt gagccgcttt ggtattaaat acttcttgtg
     5161 ctcgttcgtt tagatactct ttgatctcct gagctgtttt tccttcgata tctgcaagag
     5221 taatgctatc ttcatggaca agagtactgc cggcaaaatc gatgatactt tccaaattcc
     5281 aattttcttt ctctaattgt gtatgcgcat ctactacacg actgattgtc cgtttgacca
     5341 tgttcattag tggcttggat aagctttttt cttccatgat tacttcttgt cgctgtttat
     5401 agattacttc acgttgttca cgcatcacat catcgtattg taagacgttt ttacgtgtgt
     5461 catagttatt tccttctaca cgtttttgtg cagattctac ttgtttcgtc agcatcttgc
     5521 tttggataac agcatcttcc tcaccgatct tcatccgatc taagaaagct ttgattcgtt
     5581 ctgaaccgaa tcgtttcatc aaatcatctt ccaatgacaa gtagaactgt gacataccag
     5641 ggtctccttg acgtcctgca cgtcctcgaa gctggttatc gatacgacga gattcatgtc
     5701 gttctgtccc gataactgct agaccaccaa gttctcttac cccaagtcct aacttgatat
     5761 ctgttccccg accagccatg ttggtagcaa tcgtcacagc acctttttga ccagcattca
     5821 tgatgatctc tgcttcttta aagtgatttt tcgcattcag cacttcatga ggaatacgtt
     5881 cttgattcag catatctgat aataactcag atgtttcaac cgcaacagtt ccgactaaca
     5941 caggttgacc tttgcggtag cgatctttga tatcttgcac gacagcgtgg aacttgcttt
     6001 gtaatgttgg gtaaagcaaa tctggacgat catcacggat aaccggacga tttgtcggaa
     6061 tctggtatac ttggatgtta taaatttctc tgaattcttc ttcttctgtt ttagctgtac
     6121 cagtcattcc cgcaagtttc ttgtacatac ggaaatagtt ctggaaagtg atcgtcgcca
     6181 tggttttcgt ttcgtcttcg atctctacgc cttctttagc ttcgattgct tggtgaagtc
     6241 catctgaata acggcgtcca tccatgatcc gaccagtgaa ctggtcaaca atcagtactt
     6301 ttccgtcttg gacaacataa tcgatatcta aaagcatgat atagttggct ctcaatgctt
     6361 gatccaagtg atgggtcaat gcagtatttt caatgtcgta taagttatcc aaaccgaatg
     6421 tttcttctgc tttttcgatc cctgcttcgg ttaaactgat tgtttttgat tgtacatcga
     6481 ttttataatc ttcttcttct ttcaaacgtt tcacgaagtt gtctgctctt gtatataaag
     6541 ctgtggattt ttctgcttgc cctgaaatga tcagcggcgt tctcgcttca tcaatcaaga
     6601 ttgagtcaac ttcatcgaca atggcataat tcagcggacg ttgtaccatt tgatggcggt
     6661 aaacgaccat attgtctctc aagtagtcaa atcctaattc attattcgta ctataagtga
     6721 tatcacaatt ataagcctcg cgtttttcat ccgaagattt tgagttgata ttcaacccca
     6781 ctgttaatcc taagaaatta taaagttcgc ccatttccgt cgagtcacgg gttgctaaat
     6841 attcattgac ggtaactacg tgtacccctt cgccagtcag agcatttaga tatactggca
     6901 tcgttgcggt caaggtttta ccttcaccag tacgcatttc agggatattt ccatcgtgaa
     6961 gcacgattcc acccatcaat tgtacgtggt atggatataa acctaataca cgttttgctg
     7021 cttctcggac gactgcaaaa gcttccggaa gtaattgatc taatgtttct cctttttggt
     7081 atctgccctt gaattcagca gtttttgcct gtaattgttc atcagttaat gctgccattt
     7141 ggtcagcgta tgcttcaact ttttttgcaa tgctatctaa acgttttagt tctttcttat
     7201 cgttttcgat aagtgaacga atcaaattgg ccatgcgttg tgtaatctcc ttttccttct
     7261 gtgtattatc cagactatgt agac
//
LOCUS       DADVXO010000119         7055 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.123, whole genome shotgun sequence.
ACCESSION   DADVXO010000119 DADVXO010000000
VERSION     DADVXO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.123"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            35..220
                     /locus_tag="KB950_002336"
     CDS             35..220
                     /locus_tag="KB950_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858516.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            725..919
                     /locus_tag="KB950_002337"
     CDS             725..919
                     /locus_tag="KB950_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858517.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1041..1359
                     /locus_tag="KB950_002338"
                     /pseudo
     CDS             1041..1359
                     /locus_tag="KB950_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1603..2433)
                     /locus_tag="KB950_002339"
     CDS             complement(1603..2433)
                     /locus_tag="KB950_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBB6858518.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2641..3975
                     /locus_tag="KB950_002340"
     CDS             2641..3975
                     /locus_tag="KB950_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6858519.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4016..4927
                     /locus_tag="KB950_002341"
     CDS             4016..4927
                     /locus_tag="KB950_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858520.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4924..5778
                     /locus_tag="KB950_002342"
     CDS             4924..5778
                     /locus_tag="KB950_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858521.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5775..6392
                     /locus_tag="KB950_002343"
     CDS             5775..6392
                     /locus_tag="KB950_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBB6858522.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6394..6912
                     /locus_tag="KB950_002344"
     CDS             6394..6912
                     /locus_tag="KB950_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBB6858523.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2123 a         1207 c         1407 g         2318 t
ORIGIN      
        1 ctataataaa agttatttaa ggaagtgtct atgaatgagt aaaaattcta aaggattcct
       61 aaccatctta ttagctttta tagggtatat gttggttggg ctgctaaaat catacagtaa
      121 tgagttgctg aatttttcaa catttattaa tgatacatta gttccaagtt tgtttttcat
      181 tgtatttttt gctgttggtt attttataat aaagatatga tgactaattt agctcaacta
      241 ttccagcgtc agctgaagat attgcaattc ccgattcctt aatcatttat ctggatgaga
      301 cagaaagtga agttgcaaca gatgtgcatg tagttgagga taaaaatgat agtttaaaaa
      361 ttaatagttc agatacttta ctaagagtaa gtcctatctt ttcagctgtt atttagatgc
      421 aagtagcaac taagttaggt agtactcctg caatggatag gtatttgtgt gttcgatata
      481 taagtggtcg aagtatcaag gttatgtata ctggatggga gatagaagag ttaaaaattg
      541 gaggtcaccg ccacaaatgg aatttagatt tagaggtacc ttagatttgg tatagttatt
      601 aaacacaaga tggcctggtt tgtagggaaa gtcgaagagt atttttccac ttctgcgccc
      661 ctttaatttt tctagtaatg ccgtgaattt gtgattaatt ggaaatatga agaaaggaga
      721 gtctatgaaa acaacactag ttctattcta taaaaagcat ccttatttta cacttctgat
      781 aaatatattg ttggctagtg ttatcgggat ttctgttgaa tatctaataa ataaagattt
      841 cataggttct tgtttttata cggctttatt tcttggactt cttgaagcat tttccattta
      901 taaaaaatca aaaaaataaa atagattgcc gtaagagatc tttttcattt catttttttc
      961 tgaaaacccg ttgtttctgt cagtgaagca gcgggttttt agatttattg tctttagacc
     1021 taattgagcg cgccgagcga ttgaaataca aaaccacttg ctatgcgggt ggagcaataa
     1081 agggaaatag tttagcccgt acaaagtaga tgtgtaaata ccacattgta ttcacaccaa
     1141 agtatagaag aaaaataatt tataatcaat atcgagccaa tattcaggag atcatcaaga
     1201 gattgtgcaa atctaaaata taaatttggg tatcgacact tttgggtgga aggatactat
     1261 gtgacaaaag taggacaaaa tgaagcgacc atcaaaaagt acattcaaga acaagaaaaa
     1321 catgatagtg cgttagataa gctgagtgta cgagagtaag acgatccttt taagggctaa
     1381 ggagtagtac ataaaccttt taagaggtgg gcaaaactgg caaaggcaat atggcttgaa
     1441 cataaaatat gaaagccagc gtctttaggc gctggccggt gatttgagct tatagctcta
     1501 gagaaaacca cccttttgaa gggtggttat gattaatgac tttagtcagt tttgctcaca
     1561 aagcgtgtgg aacaaaaaac gggtgtttct acgggtagat atttatttct tgaatggagg
     1621 tgtcccatac atttttggtg ttactggttc taattttggt gcagcgcctt cgatcttagg
     1681 ttctttcaac cattgatatt cgccttcgcc atccagtgct ttgccactag cccaagaacc
     1741 ttcactactt tggtctccct ctgagaagtt atagaagtca taagcaatgt cttttcgttc
     1801 tttgtcacgc gggaaagtac ttgggactaa gattgaacct tctttttctt ctaattcttt
     1861 cactgcacat tcccatagat tttgatgata gctatctctt gcaaggagaa cagacagcat
     1921 atcttttacg ccacgatcat cgatcatttc gtaaatacgt gctacttgaa gtcttccttg
     1981 tgattcggca tttaaattag ctctaaagtc tgctaacaag ttgccactcg caatgatata
     2041 ggcaccgctc catggattgc ccacgctgtc agcaggacga ggtcctaagc cagctacgat
     2101 cgcgtgctgt ggattcattc cagagataat ggcaccgatt gttggatctt tttgcatcgc
     2161 tgcttcttga tcttcaacag gcgcaccctc taataaacga gcaaccattg tcgccaacat
     2221 ttccacgtgg ccgatttctt ctgttgcaat gtccatcagc atatctttat atttttcttc
     2281 tcctcggcag ctccagcctt ggaacaggta ctgcatagct acactcatct cgccatattg
     2341 gccgccgatc agctcctgca gatatttcgc gattaatgga ttcggacgat ctggttttgc
     2401 ttcaaactgc aattctttct ggtgtctaaa catcaaaatt cctcctaatc tatttttaat
     2461 acattctcat tctgctacga aagattaaaa aattcaaaga aagcgctcgc gaattatata
     2521 aagtattggt atcattaata atttttttag aatcatacct tataaattgt tattttcaga
     2581 acaaaatgat atcgttatat taaaaccggc atcggttaca ttaaaatttg gaggtaaaat
     2641 atgaaaaaat tattgtttac agggttgctg gcaagttcag cattgttttt attaggagga
     2701 tgcggatcaa gtaatgcaag cgatgccagt tcaagtgcag caaattcttc tgaaaaaaca
     2761 agtgatggaa gaaaaaatat tgatttctgg tccttttggg gatccggtgc acgaaaagaa
     2821 gctatcgaag aaatcattga ggatttcaat caatcacaaa acgagatcca tgtaaattat
     2881 aagtatcagc catggggtga catctggact aaatctttat ctgcaataac agctggaaat
     2941 gcacccgatg ttattgtaca ggacataaat tccgttgccc aaagagcaga agcacagcaa
     3001 gcaactaatc tctccaaata tgtagaaaaa gacacttcta aggattttta tccgcagctt
     3061 tgggatacag tagaatatga aggtgatcct tatgcgatcc cattcaatac agatacacaa
     3121 gtcatttttt ataataaaaa gttatttaaa gaagctggaa tcagtgaaca agatctaccg
     3181 acaacttggg atgaattaga agaaacagca cgtaaactag ataaaaaaga aggaaaagaa
     3241 tttacacgca tcggttttta tccattatgg aatttagggg ctgatgtatg ggcgctaaac
     3301 gcagataacg ggactagttg gtttgacaaa gatgaaaaag tcaagattga tactaaaaat
     3361 aaagtggaag ccttggagtg gatccaagac tggcaagatt attatgggaa aaatacgatc
     3421 aataaattag aagccgaatt tgggtctggt gtatctgatc catttatttc cggattagta
     3481 gccatgcgtg cccaaaatat caattattac tctagtttga tggaaaatgc accggatgat
     3541 tttgattttg gcgtgatgca gctcccagaa aaagaagaag gttcaggcca ttggtcgtgg
     3601 ggtggcggat tcgtcttgga agtaccatac ggagcaaaag atcctgaggc ctcttatgaa
     3661 ttcatcaaat atctttcttc tccagaagta caagaaaaat tcggcgaaaa aagttttgat
     3721 atcatggcga gcaagacagc caatgagaat ttagtgaata atgacaacct aacggataaa
     3781 ggaaaaatga tctatcaaat ggcggacgaa aactttaaaa atacggtgat cacaccagtt
     3841 cctttagatg ctccggatta cacgacatta gtgaatgaac agattgacca gattctttta
     3901 ggaacaaaat caccaaaaga aggattggca gatgcacaaa aagcagtaga aaacttagtc
     3961 aagcaaaatc aataatccat ttttgactgt ctaggtacag aggaggcaag aaaatttgaa
     4021 gcgtttatct atccgaaaga aaaaagaagc aagatgggcc tatttattta tctcgccttt
     4081 tattatcggg ttcttggtat ttatgctagg gcctatgctt ttttcagtta ttggtagttt
     4141 caccgattat aatttgacat ccaagatgaa tttcattggt ctagcaaatt tcaaaagaat
     4201 gttcgttcac gatgatttat tttggaaatc gttatacaac accgtttatt tcgtcatctt
     4261 caatgtaccg ctgacgacga ttttcagtgt gtttttagcc acagtactca atcaaaaaat
     4321 caaaggaatc agtgtattcc gtacagcttt ctatttaccg gcagtcttat caggagtagc
     4381 agtctacatc ttatggatgc agctgctttc tccatctgca ggattgatca acacatttct
     4441 gggctggttc catatccctg gaccagcttg gctgtttgat ccacaatgga cgaaacctgc
     4501 gttgattttg atgaaaatat ggagttctgg aggagcaatg ctactttact tagctacatt
     4561 acaaaatgtt cctagatctt tatatgaatc agcagaaatc gatggagcag gcgtttgggg
     4621 aaagttcaaa agtattactt tgccattaat cactcctatt attttttacg atgtgatcac
     4681 ctctacgatc ggttctttcc aaatttttca agaagcttat gtcatgacga aaaatggaac
     4741 aggcggacct gcaaattctt tgctttttta taatctgcat atgtggaaca aggcgttcgt
     4801 agcttttgat atgggttatg cgatggctat gtcaatgatc ctgtttctga tcgtgttggt
     4861 tctgacgttt gtcaatttga agcttgcacc taagtgggtc tattattctg gaggtgacgg
     4921 caaatgatgg attatttcaa aaagaccagt gtgaatcggc aaacaaaaaa aatactaagt
     4981 tatgtcctca tgacagtaat aggaatcatt ttgatcattc cattgctttg gatggtgttt
     5041 acttcattga aaccaatgga agaaatcgtt cgttatcctc cgaccttttt cccagaaaaa
     5101 atcgtatggg aaaactatct ggatacgatt gccgcctttc ctttttggcg ctatgcaaga
     5161 aatacattat tcatcactgt gctagtagtg atcggtaatg tcctttcaaa ttcttttatt
     5221 gcttatggat ttgcgaagct tgattttcct gggaaaaagc tgatgtttgc tttggttctt
     5281 tctacgatga tgatcccggg gtttgtaacg atgatccctc aatatgtatt attctccaag
     5341 atcggctggg taggtactta tttgcccctg atcgttccat cgttcttcgg caatgcgttc
     5401 aatatatttc tgatgcgtca attttactta tcgatcaata atgaattgat cgaagctgct
     5461 gaaatcgacg gagcgaatca tttgtatatt tggagtcatt tgatgcttcc attgacgaaa
     5521 cctgccttga ttaccattgc gatcaattcc ttcaacgcag cttggaatga tttcttaggt
     5581 cctctgttat atatccaaga tcaagaaaaa tacacgttac agattggctt gcaagttttt
     5641 cagaaccaag caacgacaca atggaattat ctgatggcag gagcaacact tgtcttgatc
     5701 ccgacgattt tgctgttctt ttttgcacaa cgttatttca ttgaagggat ggatttgaca
     5761 ggaggttcaa aaggatgatg aatcgatcga ttggtaaaaa agccaatgat tatttcttag
     5821 aaatcattga atggattcca cgtcttatcc atttacagct actatggttc ctgtgcgtct
     5881 tgcctgtttt cacattagga actgcaagtc gaacagttct gtatatgatt tatcattaca
     5941 aaaaaaatga agagaaaaag ctcggttcac tattctggaa aaagtttcgt gagaacttct
     6001 ctctttatgg aaagcaagat agtttagcaa gtgtttattt tcttcttcta ttgatcgatt
     6061 atcagatttt ccgctattgg ggtgggggaa ttggctatac attgatgtat actacgttgt
     6121 ttttgcttct attaagcgcg cttctcttta gttacaaaat cttgcttcaa ctggaacaaa
     6181 aagaagcttc ttggattact gcatttttct tgttttttaa tgatttcaaa agcgcgcttt
     6241 tctatttggc aggcacgttg atccttcttg tcgcattctg gtttgcagga ccaatctatt
     6301 ttgctttatt aggtttcagt ttcttgtttt attttcaagt cctgcttttt atccatcgta
     6361 cgcaagaaaa ggttcttaaa aaggaggaat aacatgttgc gtttgattgc taaaatcgat
     6421 gatagtatta tcaacaagtt cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt
     6481 ttgaaattta tcagtaatat ctctaatggt ggttttattt ggttgctcct tgctgctgtt
     6541 ttagtcttga tacctgatac tagagaatat ggtctagcca ttcttatcgg cagtttgatc
     6601 tatttttttg ttggtttgct ttttatcaaa aaaacgacaa gacggaaaag accttatgaa
     6661 aaaaatcgtc ggcttgtcct ttacacaagt aaaccgaaga cttcttcttt tccttcagga
     6721 catacagcgc tagccttttt atcagtagct gcgttgttcg aattaagtcc agttgcagga
     6781 atattagcct ttttatttgc ttgtttgatc gcttattcga ggattttttt acttttacat
     6841 tatcctactg acattttagg aggagctatt ttaggaagtg ttattggcat cctatgcgct
     6901 gccgttatct gaattaattg tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt
     6961 tatttttttg tttcgaggaa agcgtagttg tttattcatt tgtcttaata acttaagttt
     7021 tgttaaatga gaacgttata aaattttata attta
//
LOCUS       DADVXO010000120         7005 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.124, whole genome shotgun sequence.
ACCESSION   DADVXO010000120 DADVXO010000000
VERSION     DADVXO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.124"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            178..1035
                     /locus_tag="KB950_002345"
     CDS             178..1035
                     /locus_tag="KB950_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBB6858524.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1028..1930
                     /locus_tag="KB950_002346"
     CDS             1028..1930
                     /locus_tag="KB950_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBB6858525.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1963..2313
                     /locus_tag="KB950_002347"
     CDS             1963..2313
                     /locus_tag="KB950_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6858526.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2362..3285
                     /locus_tag="KB950_002348"
     CDS             2362..3285
                     /locus_tag="KB950_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6858527.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3363..4256
                     /locus_tag="KB950_002349"
     CDS             3363..4256
                     /locus_tag="KB950_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6858528.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4294..4857
                     /gene="amaP"
                     /locus_tag="KB950_002350"
     CDS             4294..4857
                     /gene="amaP"
                     /locus_tag="KB950_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBB6858529.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4871..5059
                     /locus_tag="KB950_002351"
     CDS             4871..5059
                     /locus_tag="KB950_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBB6858530.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5072..5974
                     /locus_tag="KB950_002352"
     CDS             5072..5974
                     /locus_tag="KB950_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6858531.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6006..6248
                     /locus_tag="KB950_002353"
     CDS             6006..6248
                     /locus_tag="KB950_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6858532.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6504..6875)
                     /locus_tag="KB950_002354"
     CDS             complement(6504..6875)
                     /locus_tag="KB950_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858533.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2554 a         1189 c         1391 g         1871 t
ORIGIN      
        1 tacctttgaa taaaaagcca tgaaaaacga atagttagcg cttaccgatg cgttttgtga
       61 taaaaaaatt ttaaaatatt caatgaaaac aaagcatata ttttgcttta aaaccaaatg
      121 aggaatattc tagatgtaga acaggttaga aaaagattag aaaaaggagt gattttgatg
      181 gagaagagat acggcgtagt tcctgtggta actgcttcag acgaaaatta tgctccttac
      241 ttgagcgtga tgatcgcaac tgcattagaa aattgcaata aaacaagacg cataaaattt
      301 tatgtgattg acgatggtct atcagaatat agtaaagaag gattggaaga aacggtaaat
      361 aaatattcaa gtaatgcaag tatccaattc ctgacagtgg aaaaagatat ctacgaagac
      421 ttcctagtca gtgaccatat cacaactacc gcttacttaa gaatttcttt accaaactta
      481 ttagctaaag aggattataa gaaagtcctt tacttagatt cagatgtgtt agtactagac
      541 gatatcgtaa aactatatga tgagccgtta aatggaaaaa caatcggcgc tattatcgat
      601 ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt gtacttcaat
      661 tcaggagtga tggtgattga cattgatcaa tggaataaaa aagaaattac agaaaaaact
      721 atccattacc tcagtgaaaa tggcgatcga atcatctatc acgaccaaga tgctttgaat
      781 gctgtacttt atgaagattg ggaacaactt catccaaaat ggaacatgca gacttcgctg
      841 atttttgaac gtcatccagc accaaatgaa aaatatgaac gtcagtataa agaaggaaat
      901 gaaaaacctt cgatcgttca ctttacagga catgataaac cttggaatac gttgaaagat
      961 catccgtata ccaatttata cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc
     1021 gagatcaatg aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc
     1081 acatttagct gcattatttg tctcagtatt agataattgc aacccgtcta aatttgttcg
     1141 gttctatgtg attgacgatg atatcgattt tgaaagtaaa cagcttttac gcttctcagt
     1201 gaaaaacgca cgaatgaact cagatgtgga attcttgaaa atcaataaag aattctttac
     1261 aaatgtggta atcagcgatc gtattccaga aacagcttat tatcgtattg cgattccgga
     1321 attgttccgc ggcacagaag ttgaacgtat cttatatatg gattgcgaca tgatcgcttt
     1381 acaagatatc tctaaattat ggcgtcttga cttcggtgat tcaatcgtag cagctgttga
     1441 agacgctggg ttccatcaac gactggaaaa aatgaaaatt tcagctaaat caatgcgtta
     1501 tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca
     1561 aaaagtatta gatttcatcg aacataatcc agaaaaatta cgattccatg accaagatgc
     1621 attaaatgct atcttacatg accgttggtt accacttcat ccgcgttgga atgcacaagg
     1681 ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga
     1741 aactcgcaac aatccttaca ttatccattt ttcaggacat gtgaaaccgt ggagcaagga
     1801 ttttgaaggg ccaacgaaga aatattacga aaaatatgca gggatgacgg ctttccgttg
     1861 tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt
     1921 tgttaaataa tagaagacca caactgaaga gggggagtag gaatggaaat tacaatgtct
     1981 aaaaatgcag tagagacgtt aattgaaaag gttggggaaa atacaaaaat cgcactcgcg
     2041 cttatcaatg attccgatcc atttctaagg gacaaaggtg cttttgcaaa aggcagcttt
     2101 tttcaaatca ttccttttgt ttcggaattt ggagaatatg cgactaaaat cgagcatcca
     2161 ttattggaca tctatacatc taaattagag caaaattatt tcggtaaacg attgaacatg
     2221 gacttcaaca aacagcttga cagcttttca ttagaaaatg aaatcgcagt actcgattat
     2281 aatatcaaat taaagaactg cttcttcagc taatagtttc gagaaagtcc aaaaaggagg
     2341 tatacattga gatactactt tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa
     2401 aaatataatt ggctcgtact tgatagtgca aagcccgaag agattgagca agcaatcgaa
     2461 gaattcgagc ttcctgatga tattttcgta ggaaccgaat atccggaaga agtttctcgg
     2521 atggagcgcc tttatgagac gaaactagag catccatttt ctttggtagt gattaatcta
     2581 gatcagtcag ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat
     2641 aatctcttga ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag
     2701 catggaaatc atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac
     2761 acccattacg tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca
     2821 gctcgtaaaa caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc
     2881 gtatatatcg atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct
     2941 gaaactttca tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag
     3001 cgacaaacag aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta
     3061 ttcaacggga tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca
     3121 ttaatcattt ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta
     3181 ccgggtaaaa gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca
     3241 gtagttttag gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa
     3301 caggtatcaa acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag
     3361 caatgacaga accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta
     3421 aacaagatca ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag
     3481 aagaatccta tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag
     3541 attcagggat cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc
     3601 ttcaattctt cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagatg
     3661 caggacgtaa gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa
     3721 tcatagataa agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac
     3781 agcaaatttc ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta
     3841 tgataaacat catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag
     3901 gaggagcaat cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag
     3961 attacgcagc aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg
     4021 cacctaaagg aatccgtgta aatggtgtag cacctggtcc aatttggaca ccattacaac
     4081 tagacgaagg acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac
     4141 gtgcaggtca gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa
     4201 gttatgttac agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag
     4261 ttgatcgttt cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga
     4321 gtgatcggaa cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc
     4381 tatccattgc cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg
     4441 caacaatatg tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt
     4501 gttttcctgt tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag
     4561 ttgacgttgg ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa
     4621 ttcatccatt caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta
     4681 aaaggggagt tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa
     4741 atcgaaaatg aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa
     4801 tacacaggct ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg
     4861 gggaccaaaa atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact
     4921 tggattgata ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt
     4981 gatcatggca gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga
     5041 tcactatttt aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa
     5101 taaaatgaac aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc
     5161 aaatggtgtg aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt
     5221 agtccctaac gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc
     5281 tcatcatcca ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc
     5341 taaacctgtg gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag
     5401 ttctgaccac acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa
     5461 gcatgattct gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt
     5521 gcaaaaaatc atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg
     5581 tttcttctct aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga
     5641 aacagaagtc ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa
     5701 agatatcgaa aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat
     5761 gacacattta gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga
     5821 atttgaaaaa gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg
     5881 tgaatttgct tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa
     5941 agtaaaagaa aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac
     6001 gaaacatggg atttatttgg tcacttatcg tcggaggaat tatcggagca attgcaggag
     6061 caatcacgaa taaaggttct tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg
     6121 gatcagcact tggtcaagca ttattaggtt cttggggacc aagtattgca ggaatggcaa
     6181 tcgtgccatc attgattggt gccatcattt tagtactaat cgtatcattc tttgctcgaa
     6241 aactgtaaaa agacttttaa aaagaggtcg tgaaaaagct gttcttcatc aagtaataag
     6301 aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttgggcatat tatcgaagat
     6361 gcaagctttt gaacactgtt tatacggaat aagaggctgt gacaagactt ttgtcacagc
     6421 ctcttattta tgcgggcaaa ttcccaatta gatttttaaa agtttttcct taaatgtgtc
     6481 tttattgata cagagaagct tctttaataa aacaatgcct ccctaaattt tcttgagata
     6541 ggaaaggtga acatacaaaa taatacgacc actttgaaaa cccagatgat ccacaaatgg
     6601 atcgtatgat actgatgaag taaaaagcaa tggatcagaa actcaaagag attgaaaaag
     6661 acaaggaaga aataatatct cttttttact cttgtctgtt ttagccaata atagctcgta
     6721 aagtaatcta gacaaacaaa tagaagtaaa caaagtttcg taagagcagc aatcgtatca
     6781 tgttgctctg ctagacgatt gacgagcagg aaaaatgttg gtaatggaat atagatcacg
     6841 agtaatgaaa gaaaaaagta aacaataaat ggcatactat tcctcctaat tgtgtgagtt
     6901 cgccctcatt ttaaaataac gatatttgtt tgtgaagctt tttcgtgtcg taacaaatgt
     6961 tatctaatgt atttttaaat aaataatcat tattatcaaa ataat
//
LOCUS       DADVXO010000121         6927 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.125, whole genome shotgun sequence.
ACCESSION   DADVXO010000121 DADVXO010000000
VERSION     DADVXO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.125"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            114..587
                     /locus_tag="KB950_002355"
     CDS             114..587
                     /locus_tag="KB950_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBB6858534.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            584..2716
                     /gene="feoB"
                     /locus_tag="KB950_002356"
     CDS             584..2716
                     /gene="feoB"
                     /locus_tag="KB950_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBB6858535.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2718..2852
                     /locus_tag="KB950_002357"
     CDS             2718..2852
                     /locus_tag="KB950_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBB6858536.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2946..3590
                     /locus_tag="KB950_002358"
     CDS             2946..3590
                     /locus_tag="KB950_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6858537.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3777..4970
                     /locus_tag="KB950_002359"
     CDS             3777..4970
                     /locus_tag="KB950_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6858538.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4963..6690
                     /locus_tag="KB950_002360"
     CDS             4963..6690
                     /locus_tag="KB950_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBB6858539.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2227 a         1221 c         1557 g         1922 t
ORIGIN      
        1 cgagagtatt ctatgtgaga atgattctca tttatttgcg taacacgtaa gaaaattaga
       61 acatataatg aatgatttta atacatgatt tatttcacaa gagaggaatc aatatgcaga
      121 ccttagcgca ggcaaagcag aatttcgttt atcaagtcga acgaatcgaa acagaagaaa
      181 aaacaaaacg ccatttgcaa aatatcggga tcgttgccgg aagtaaaatc gtcgtcgtca
      241 atttatcagg caacaatgcc attttactgg taaaaagtca tcgagtggca gtaagtcggg
      301 aaatactagg tcaaatcttt gtcaataaaa agactgcttc ctctgaaagc tggacttctc
      361 ttgatcaatt aaaagcagga gagactggga tcgtcgtagc aatccacgga caaggcgcag
      421 tgaagcgacg gttaatggat atgggattga cgcgaaatgt aaaaatagtt atccgaaaac
      481 tagcccctct gggagatcca atcgaactga cagtgcgagg gtatgaactg acgttacgaa
      541 agtcagaggc cgaactaatc ttgatacaaa gggaggaaaa agaatgaata tcgcgttagt
      601 cggaaatcca aacagcggga aaactagctt gttcaatctc ttgactggtt cgaatcaata
      661 tgtagggaat tggccgggcg taacagtaga aaaaaaagaa ggatcgttga aaaaaaatcc
      721 gaacattcat attcaggacc tacccggcat ctattctttg tctccttata caccagaaga
      781 agtgattgca agagattatt tggttcagga acagcctgaa ctgatcatca acattattga
      841 tgctaccaat ttagaacgaa atctttattt aactacgcag ttattggatt tagacattcc
      901 tatgatcatt ggtctgaata tgatggatgt catcaaaaaa gaaggaaaaa aaatcaatct
      961 agaaaagctt tcctatggtc ttggtgtacc agttttacca atcagcgtat tgaaaaagca
     1021 aggaatcgaa aaactaatcg aaaagattca ggagtctgca aaaaagcggc cttctattcc
     1081 tgttccctgt acatatgatc cacgtttaga agcagcttta catgaaattg aagatgtact
     1141 aggagaacaa agcggcaaga aacgctggta tgcaatgaaa tattttgaac gagatcagaa
     1201 ggtaagagag gattacgaga tagcagtttc tcagcaaaaa gagatcgagc aactgatcca
     1261 gttaacagag aaactattgg acgacgatag tgagacgatt ctggtcaatg aacgttatga
     1321 atttattacc caactatgta cactctgtgt tgtgtcaaat gattctttcc aattatcgat
     1381 gagtgacaaa atcgatcaga ttgcaacgaa tcgttggtta gccttgccga tcttcgcatt
     1441 tgtgatgtgg ctgatctatt atctggctat tcaaacagta ggaacaatgg ggacagattg
     1501 gatcaatgac acattgtttg gtacatggtt accagaacat gttggcagat tgttagctga
     1561 atggcaagta gcgggatgga tgcaggagct gatcttgaac ggcatcatcg caggcgtggg
     1621 agcagtgtta ggatttctac cgcaattgat tatattgttt ttatgcttga gctttctgga
     1681 agactgcgga tatatggcga ggatcgcttt tgtcatggat cgacttttca ggaaattcgg
     1741 tctttcagga aaatccttca ttcctatgct gattgctaca ggatgtggag tgccaggcgt
     1801 catggccagt cgcacgattg aaaacgagca agatcgtcgt ttgacgatca tggtcacaac
     1861 cttcatgcct tgttctgcaa agttgccgat tattgcctta gtagcagggg ccttttttcc
     1921 tcatcaatct tgggtagctc cttctgccta ttttcttggt atgactgcaa ttattttctc
     1981 tggaatcagc ttgaaaaaaa cacgtttgtt tgctggagat acagctcctt ttatcatgga
     2041 attgcccgca tatcattttc ctcaatggtc agcggtccta agacaaacct atgaccgcag
     2101 caagtctttt gttaaaaaag caggaacgat tatctttgtt tctagtatcg tgatctggtt
     2161 cagctcttct tataattttt atgctcaacc tgtttctgaa gaccaaagta tcttagcctt
     2221 tttcgggcga atcctggcag ttgtttttca gccgcttggt tggggaaact ggcaaggaac
     2281 agtcgcagcg attacaggat tggtcgctaa agaaaatatt attggcacat ttggtatttt
     2341 gtttggtcat gcggaagtat ccgaaaatgg cagagaaatc tggcaagtgc tacaacacac
     2401 atatactccc gcagcagctt attcattgtt agccttcaat ttactttgtg cgccttgttt
     2461 tgcggcaatt ggggctatcc atcgggaaat gaatgccaag aaatggactt ggatcgccat
     2521 aggctatcaa tgcggtttag cttatttagt tagttttgtg atttatcagt tgggacactt
     2581 attggagggt ggacaggtag cgttagggac gtatcttgct attttactga tgataggact
     2641 cgtttatgtt ttgttccgtc agccaaaatc caagaaccag ttttatacta tattgtcatt
     2701 agaaggagag gaataggatg gcgacattct tattaagtgt gttaatcttt ggaagtgcag
     2761 gcgcaattgt ctatactcgt ttaaaatccg gtaagaattg tgatgactgt caaacagctt
     2821 gcccagtaaa aaaggaacag atcaagcgat gatcagtcgt tgcaagaggc tattctcccc
     2881 ttttttgctt attttgctag agcatagtaa gatgagggaa agtaaaaaaa gaaagaggaa
     2941 agaaaatgtc tggaaagaaa aaaatcgtcc gcccgcgcta ccagcagatt gctgttgatt
     3001 tagccgaacg tatcgttgaa aaccgatata aagtgggtga gaagatccat gcccgctcaa
     3061 cacttgccag tacgtacaat gcttctcctg agactacccg caaggctatc aatgtgctgg
     3121 tagatcttgg aatcatggaa gtacggcatg gaagtggggc atttgttgct tcgaaggaaa
     3181 aagcaaaaga tttcctattg caatatcaag atgtacaatc catccaagat acaaaagcag
     3241 aaatcatggc aagtgtggag aagcaacaag atgagctgaa tcatttttct caattattgg
     3301 atcagctggt caatcaaacg aagcgaatac ataagatgaa tcctttactt ccgtttgaat
     3361 tatctttatc agaaaaagca atgcatctgg accgttcaat tagcgagttg aatatctggc
     3421 aggcgactgg cgccacagtt atcgcaattt ctcataatga agaactgctt gtttcacctg
     3481 gcccctatgc taaatttacg gcaggggaca tcgttcattt tattggaaat gaatatacac
     3541 tacagcgtat gacaaacttt ttttacccgg agcatcagct tgcagaatga ttgtgtgaaa
     3601 tattaaaaaa atattaactt ggctaaacct tttatttaca aagatttagc caagtttttt
     3661 tcagtttgga caagcaaatt tctttcattt ttgacaaagt gactacatga tgttaacata
     3721 gtgtggtcac ttttttcaga aagaaaaatg aatttacata aaaaaggaga atttgtttgg
     3781 taaaagtaga agtaaaacat ttaacaaaga tatttggtaa aaagacacaa gccgctttag
     3841 atatgatgaa tgatcaccag ccgaaaacgg agattttaaa gaaaaccggt gcaacggttg
     3901 gtgtgtatga cgtgaatttt gacgtaaaag aaggagaaat cttcgtgatc atggggcttt
     3961 ccgggagtgg gaaatccaca ttgatccgtc tattgaatcg tctgattgag ccaacttcgg
     4021 gcagtatcta tattgatgga gaagatgtat cgaagttaag caaagaagaa ttgcgagaag
     4081 tccgtcgtca taaaatcaat atggttttcc aaaacttcgg tctgtttcca catcgaacga
     4141 tcttggaaaa tacagaatac gggttagaag tccgtggtgt acctaaagaa gaacgacaag
     4201 aaaaagcaga aaaagcatta gaaaattcta gtttgctttc atttaaagac caatatccta
     4261 gtcagttatc tggtgggatg caacaacggg ttggcctagc cagagcttta gcaaatgatc
     4321 ctgagatctt gctgatggac gaagcgttct cggctcttga tccgctgatc cggcgagaaa
     4381 tgcaagacga attactggac ctgcaagcca atgttcaaaa aacgattatt ttcattaccc
     4441 atgatttgaa tgaagcttta cggattgggg acagaatcgc cttgatgaaa gacggcgaaa
     4501 tcatgcagat cggaacaggt gaagaaatat tgaccaatcc agcaaatgat tatgtacgtg
     4561 aatttgtgga agaagtcgat cgttccaaag tgcttacggc tcaaaacatc atggtacctg
     4621 cattgacgac gaatatcgaa tctgatggac caaacgttgc attgacaagg atgcgtaatg
     4681 aagaagtcag catgctgatg gctgttgaca gaaagagaca cctgaaaggg atcatcactg
     4741 cagatcaagc gttagaagct aggaagcaaa aacgtccatt gatcgacttc ctagatgaaa
     4801 atgtgacggt gattgggaaa gacatggtgg taagtgatat tttcaatatt atttatgact
     4861 caccgactcc acttgcagtt gtagaggatg gaaaattaaa aggtgtcgtc atccgaggta
     4921 gtgtgattga agcattagca gaaacaagcg aggtgagtga acatgaataa tctattgaac
     4981 tatcaattac cagtggcaaa ctgggtagaa aatatcacag aatggtttac gacaactttt
     5041 tctggcctat tttcattttt acaaacgatt ggacaggcag tgatgtcggg gattacaaat
     5101 ttactgttag tgattccagc tcctttgttt atcttgttat tgacggtagc agctttcttt
     5161 atttctaaga aacgtccagg acttacgttg ttcacattaa tcggtctatg gtttatttat
     5221 aaccaaggtc tatggaatga tttgatgaat actgtcacac ttgttctttt atctagtgtg
     5281 atctcgatca tcattggggt accattaggg atcttgatgg caaaaagcag taaagcccaa
     5341 agtattatca aaccgatcct tgactttatg cagacaatgc caggattcgt ttatttgatt
     5401 ccagcagttg catttttcgg tatcgggatg gttccagggg tatttgcctc agtcatcttt
     5461 gctctgccac caaccgttcg ttttacgaat ttagggattc gacaagttcc taaagaatta
     5521 gtagaagctt ctgattcatt cgggagcaca agtcgtcaaa aactattcaa actagaattg
     5581 cctttagcta aaagtacgat catggcaggg atcaatcaaa caacaatgct gtcactttca
     5641 atggtcgtta ttgcctctat gatcggtgca cccggcttag gacgcggcgt attatcagca
     5701 ttgcaaagag cacaagtcgg gaatggattt gtcaacgggg ttgcattagt tatcttagca
     5761 atcatcattg accgttttac acagcatttg aaccagccaa acaataaaaa agctgcagga
     5821 gcaacaacac agaaaagtaa aaaacgtcaa ggactgatca ttggtgctgt agtagtagtg
     5881 atcttaggtg cgattggtat agggagtttt tcatctgcca aagaaacaaa acgtatcaac
     5941 ttatcttatg ttgaatggga tacagaagtt gcttcaacaa atgtagtcgg tgaagtattg
     6001 aaacaaatgg gctatgatgt aacgatgacg ccattagata attcaatcat gtggaaatct
     6061 gtttcaaatg gcgagtcaga tgccatggtt tcagcttggc tacctaaaac tcatggttca
     6121 caatatgcac aatataaaga tcaagtcgaa gacctaggtg ccaatttgac aggagcaaaa
     6181 gttggtttag cagtgccagc ctacatggat gtaaattcga tcgatgagtt aaccgatcag
     6241 gcaggtaaga aaatcatagg aatcgagcca ggagctggtg tagttacggc tgctgaaaac
     6301 acgatccaaa aatatgacaa cttgaaagac tggaaagtag agacatcttc ttcaggagca
     6361 atgaccgttg cactaggtca agcgatcaaa aaacacgaac caattgtagt tacaggatgg
     6421 acacctcatt ggatgtttgc caaatatgat ctgaaatact tagaagatcc tgaaaacgga
     6481 atgggaagcg aagagcagat ccatacgatg gttcgaaaag gattgaaaga agaccagcca
     6541 gaagcttaca aagtattaga caatttccat tggtcagaaa aagatatgga aaaagttatg
     6601 ttggaaatca acaatggaaa agatccacag caagcagcaa aagactggat caaagagaac
     6661 caagaattag tcgaaagctg gaaaaaataa aaagacagac acgaaataaa tggagcttcc
     6721 aaaattggat caaatatcca aatttggaag ctccatgttc gtctctatct tttttgtcta
     6781 taaaaaaaga gaatgcgtag ctcatcttcg aaacgttcaa agacgccgac aaactattat
     6841 gaatttatgg gtgcgagtca aagagatcga tcgtaggtat gaaagactaa tgtgattaaa
     6901 gtttattatg tacgaagtgc attctca
//
LOCUS       DADVXO010000122         6872 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.126, whole genome shotgun sequence.
ACCESSION   DADVXO010000122 DADVXO010000000
VERSION     DADVXO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.126"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..182)
                     /locus_tag="KB950_002361"
     CDS             complement(<1..182)
                     /locus_tag="KB950_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6858540.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="KB950_002362"
     CDS             517..951
                     /locus_tag="KB950_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6858541.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="KB950_002363"
     CDS             974..1294
                     /locus_tag="KB950_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858542.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="KB950_002364"
     CDS             1435..1626
                     /locus_tag="KB950_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858543.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="KB950_002365"
     CDS             complement(1697..1954)
                     /locus_tag="KB950_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBB6858544.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="KB950_002366"
     CDS             2112..2375
                     /locus_tag="KB950_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858545.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="KB950_002367"
     CDS             2399..2587
                     /locus_tag="KB950_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858546.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="KB950_002368"
     CDS             complement(2670..2855)
                     /locus_tag="KB950_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858547.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="KB950_002369"
     CDS             3070..3441
                     /locus_tag="KB950_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6858548.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="KB950_002370"
     tRNA            complement(3591..3663)
                     /locus_tag="KB950_002370"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="KB950_002371"
     CDS             complement(3709..4137)
                     /locus_tag="KB950_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858549.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="KB950_002372"
     CDS             complement(4197..4955)
                     /locus_tag="KB950_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBB6858550.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="KB950_002373"
     CDS             5250..5447
                     /locus_tag="KB950_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858551.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="KB950_002374"
     CDS             5553..6035
                     /locus_tag="KB950_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858552.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="KB950_002375"
     CDS             complement(6093..6689)
                     /locus_tag="KB950_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6858553.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2328 a         1058 c         1351 g         2135 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tg
//
LOCUS       DADVXO010000123         6539 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.127, whole genome shotgun sequence.
ACCESSION   DADVXO010000123 DADVXO010000000
VERSION     DADVXO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.127"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            222..638
                     /locus_tag="KB950_002376"
     CDS             222..638
                     /locus_tag="KB950_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858554.1"
                     /translation="MQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPE
                     IHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYD
                     LIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(711..1358)
                     /locus_tag="KB950_002377"
     CDS             complement(711..1358)
                     /locus_tag="KB950_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBB6858555.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1366..2052)
                     /locus_tag="KB950_002378"
     CDS             complement(1366..2052)
                     /locus_tag="KB950_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858556.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2058..3131)
                     /locus_tag="KB950_002379"
     CDS             complement(2058..3131)
                     /locus_tag="KB950_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858557.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3353..4027
                     /locus_tag="KB950_002380"
     CDS             3353..4027
                     /locus_tag="KB950_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858558.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4032..5372
                     /locus_tag="KB950_002381"
     CDS             4032..5372
                     /locus_tag="KB950_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6858559.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5521..6507)
                     /locus_tag="KB950_002382"
     CDS             complement(5521..6507)
                     /locus_tag="KB950_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBB6858560.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1992 a         1251 c         1139 g         2157 t
ORIGIN      
        1 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
       61 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
      121 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
      181 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
      241 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
      301 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
      361 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
      421 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
      481 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
      541 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
      601 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
      661 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
      721 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
      781 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
      841 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
      901 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
      961 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
     1021 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
     1081 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
     1141 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
     1201 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
     1261 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
     1321 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
     1381 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
     1441 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
     1501 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
     1561 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
     1621 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
     1681 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
     1741 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
     1801 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
     1861 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
     1921 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
     1981 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
     2041 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
     2101 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
     2161 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
     2221 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
     2281 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
     2341 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
     2401 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
     2461 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
     2521 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
     2581 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
     2641 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
     2701 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
     2761 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
     2821 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
     2881 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
     2941 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
     3001 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
     3061 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
     3121 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
     3181 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
     3241 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
     3301 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
     3361 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
     3421 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
     3481 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
     3541 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
     3601 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
     3661 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
     3721 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
     3781 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
     3841 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
     3901 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
     3961 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
     4021 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
     4081 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
     4141 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
     4201 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
     4261 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
     4321 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
     4381 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
     4441 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
     4501 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
     4561 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
     4621 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
     4681 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
     4741 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
     4801 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
     4861 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
     4921 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
     4981 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
     5041 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
     5101 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
     5161 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
     5221 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
     5281 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
     5341 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
     5401 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
     5461 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
     5521 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
     5581 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
     5641 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
     5701 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
     5761 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
     5821 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
     5881 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
     5941 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
     6001 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
     6061 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
     6121 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
     6181 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
     6241 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
     6301 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
     6361 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
     6421 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
     6481 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgatt
//
LOCUS       DADVXO010000124         6352 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.128, whole genome shotgun sequence.
ACCESSION   DADVXO010000124 DADVXO010000000
VERSION     DADVXO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.128"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(99..1229)
                     /locus_tag="KB950_002383"
     CDS             complement(99..1229)
                     /locus_tag="KB950_002383"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBB6858561.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="KB950_002384"
     CDS             complement(1878..3080)
                     /locus_tag="KB950_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6858562.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="KB950_002385"
     CDS             complement(3140..3343)
                     /locus_tag="KB950_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBB6858563.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="KB950_002386"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="KB950_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="KB950_002387"
     CDS             4880..5692
                     /locus_tag="KB950_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858564.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1898 a         1302 c         1002 g         2150 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac ag
//
LOCUS       DADVXO010000125         6298 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.129, whole genome shotgun sequence.
ACCESSION   DADVXO010000125 DADVXO010000000
VERSION     DADVXO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.129"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(42..230)
                     /locus_tag="KB950_002388"
     CDS             complement(42..230)
                     /locus_tag="KB950_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBB6858565.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="KB950_002389"
     CDS             500..862
                     /locus_tag="KB950_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6858566.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="KB950_002390"
     CDS             914..2596
                     /locus_tag="KB950_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBB6858567.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="KB950_002391"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="KB950_002391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBB6858568.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="KB950_002392"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="KB950_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBB6858569.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="KB950_002393"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="KB950_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB6858570.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2095 a         1095 c         1436 g         1672 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcg
//
LOCUS       DADVXO010000126         6292 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.130, whole genome shotgun sequence.
ACCESSION   DADVXO010000126 DADVXO010000000
VERSION     DADVXO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.130"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..2483
                     /locus_tag="KB950_002394"
     CDS             <1..2483
                     /locus_tag="KB950_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBB6858571.1"
                     /translation="YNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSE
                     KKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLL
                     HRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNC
                     FKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGV
                     TLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVN
                     PSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLA
                     YYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHE
                     SLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLE
                     LAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRN
                     MNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMP
                     QETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFG
                     WGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQD
                     ILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHT
                     PSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRR
                     TKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2665..2904
                     /locus_tag="KB950_002395"
     CDS             2665..2904
                     /locus_tag="KB950_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858572.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2920..4293
                     /locus_tag="KB950_002396"
     CDS             2920..4293
                     /locus_tag="KB950_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6858573.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4297..5931
                     /locus_tag="KB950_002397"
     CDS             4297..5931
                     /locus_tag="KB950_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6858574.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6094..>6292
                     /locus_tag="KB950_002398"
     CDS             6094..>6292
                     /locus_tag="KB950_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858575.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2198 a         1030 c         1278 g         1786 t
ORIGIN      
        1 cttataatcc tattaaacaa gaggtgatta cacaaaaaaa gcattctgtg agagacaatg
       61 atattgaaaa aattcggttt gtatttattg atattgaccc taaaaggaaa gagggaagtg
      121 caacagatag cgaaaagaaa aaggcagaaa atgttatgga acaagtagaa cgttatttga
      181 aagaaaagag aatagaatca tttgtaaaag ttgatagtgg gaatggctac catatttttc
      241 ttcccataaa tgaacaacca aataatcacg agacgattct tactattcag aattttcttc
      301 aattacttca ccgaagattt ggagtagaag atgaagttga tattgatact tctgtttata
      361 atccttcaag attatgcaaa ttagttggta caccagcggt caaaggaaag tccacaaaag
      421 agcgtccgca tcgtttatct agtttccttt ctattcccga taacatagat agaagtaatt
      481 gttttaaaat tcttgaacag atagttgaag aaaattccat gatgtcaacc tctagagtaa
      541 aaagccatcc atcttttaga gaagtgttac cttttattca agcagactat aaacaatggt
      601 tagctcacta tcctgaatta acttatacca taaaagaggg cgatcataaa ggagttacct
      661 tacttatatt tgacgaatgt ccattaagaa aacacacgaa taattctaat ggttcttgtt
      721 tgtctgttac ggctgataat aagattcgat ttcattgtct gcatgcttcg gataaaaata
      781 aagatattca agactttgta aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag
      841 aatcattaga atccaatcaa gaatattatt ttgaccagtt taaactgaat caagaaggag
      901 tatttttggt gaaagaaggt caagaagaaa aagttgcttg tccgatgttt attaagcaaa
      961 tacgcaaaaa tatcgaacaa aaagtcatta cacttgttct tgcctattat gtcgatcatt
     1021 gttgggatga aatagaaata gaaggtggat gtttacaaac taataatttt aaacaattag
     1081 caaaacatgg cttaatcatt aaagctagaa gagaagctga agtcgttgac tttttacaga
     1141 ttcaacggga gaatgctcca atcttttttg aacatgaatc tcttggctgg aaaaaagaaa
     1201 atgaagcaat gatttttcag ttacaagatt cttttggacg tgaaattcct tctgtattaa
     1261 ctaaaaactc atgctaccaa ttaacaacta atggtacaga agcggaattt gacgcattgg
     1321 ttcataagta tgttttagga acgaacttag aacttgcttg gtcgattggt attgcttcta
     1381 ttgtgttagg ctatttaaac gttacgaagg ctaatacttt tttgagttta ctaattaacc
     1441 tacagggcaa gagcacaacg ggtaaaacca ctacgttaca ctttattgcc tctttatttg
     1501 gaaatccaac tacggttctt agaaatatga atgctaccaa taaagccatt ataaagctag
     1561 catcaaacaa tcagggaggt gttccactaa tactagatga gttaggagca gcaactcaaa
     1621 ataactttag ctcgttgatc tatcaattag cttcaggaga agaaaggcta agattagatt
     1681 ctaattgtca gcttatgcca caagagactt tttctgtttt gacccttacc tcttctgaag
     1741 agcgtttgga aaattatttc aatgagaaat taactggttt gaaagttcga tatatcgaat
     1801 tttctgatat caagtttaca aaggatgcga atcacgcaga gaaagttaaa agtctttctt
     1861 ctaatacttt tggttggggt ataaagttat ttcttttgaa actctttgaa caagggatta
     1921 caaaaattga agcaactttt gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac
     1981 attctttgaa gcaacgcatt ggtaataatc tagctttgat ttatagtgga gccctcctaa
     2041 ttcaagatat actgggctat cctttacgat tagagcatat acagacggtg cttattaatt
     2101 cgtacgaaca gttaattgta gatactagtt taggaagtga gcaaacggta tttgaccgag
     2161 tgaaaacatt gttggtgatg aatagccata aatttttaac aaacagtgac aacaaacata
     2221 cgcccagtac gatttgggga aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata
     2281 tttttcctat cccgtttgag gacttgttaa ggcgagaatt tcaagtaaag gatctaacct
     2341 atctttttag gcaattgctt gattcgggta atatgtgtgc agaaaaaggg agaagaacga
     2401 aacgtattca catgaatcgt caagttcttc caacatacga gattctatta ccttcttcac
     2461 tcaaatcgta ttttcgattg taatttagaa taaaccaaag tatgaaagtg aggcaccatg
     2521 ttatggaaat atttcttatt gatttagcaa aacaactgat tacttatctg tatgatagcg
     2581 agcaaaccca aacggaagta aatgaagctt gatctatgct tcatttactt cattcaatca
     2641 aacatgaaag aaaggaaaat tctaatgact aaaaataaaa tgacactaaa agcagaggtt
     2701 cttctgtata ttcaagaaca tttttcaaat caagcctttt ttacaaaacc aatttacttg
     2761 gatttcgaaa taagaggggt atctgctgga tctatcgggg gtactcttca agcgttgaag
     2821 aacgaaggat atttagaaaa tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa
     2881 aaatggtatt tagttcattc atagagaggg gaatcaagca tgcgtgtggc ttgttatgat
     2941 cgaacgtctg tttatgaaga agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa
     3001 gagtatgttt tagagaaata tgaacaaatt ccccaacgat tttcagacaa aggagaatca
     3061 ggggaaaaag cggagcgtcc agcttttcga cagcttagag cttgggtttg tgcgtcagaa
     3121 gaagaggggg tgatctatgt ttggcgttat gatcgattgg cacgtcaagc caaaatagcc
     3181 ttagaattta ttgagttgtg ccaacaacat caggttcaag tgtattcgat ttctgaacct
     3241 cttcctgctt ctttctcaca cgcgtcaacg aataaactat ttgtgcaatt attgttcact
     3301 tttgcagaac aacaacgaaa cgtcattatt gagaatgttc gtgccggact ttcctataaa
     3361 aagcaacaac ggcaatattt gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg
     3421 aaaatccaac aagaagtaca cgaagcaagg attgttcgct atatttttca actttatttg
     3481 accaaaaaat atggctacaa aaagctttgt caacgattga cgcaacaaaa gcttttcttc
     3541 cgcgaacgtc cttttcagcc ctatcatatt tatagtatcc taaaaaatcc attgtactat
     3601 ggcgaaatca aaggaggatc tttggggaag tatctaggaa cctttgaacc gattctctcg
     3661 aaagcaacct ttttacaagc ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat
     3721 acgtatctgt acttattacg tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt
     3781 tcgaagtatc aatggaatac aaagaagacg aagacgcttc attattatca ttgtaccgac
     3841 cgaagctgcc aaggaatttt tcttcgggca gaaagggttg agtcagaagt attggaaaag
     3901 atacaggctt tttttcaata cgaaggcgtc tatcaagcct tgattgccca attaacgcaa
     3961 caattagccg aaaagcgaaa agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa
     4021 gcacaatgga ttaaggcatt tgaagagggc aattgttcac tggaagaact caagcaacaa
     4081 ctgcaagctc ttcaaaagcc gatgtctact tcttcgtttc tttcactcca acagtgtgaa
     4141 gcaaaagtga aagaatggct tcaactaaaa gacctctcta gtcgacaatt attatgtgac
     4201 tatgtttgtc ggattgaggt aacaaaggat aaaacagttt gtgggattta cttccgacaa
     4261 ctgacacaaa atattttaga aaggaacgaa tgatctatgg aaatgataca aaccaaacga
     4321 gtcgcattgt atattcgcgt gagtacacaa gaacaagctt tagaaggaca tagtattcaa
     4381 gcacaagaac aaaatggacg acagtttgcc gaacggatgg gctatgaagt ggtagaagtt
     4441 tatgccgatg aaggaatcag tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg
     4501 atgaacgatg ctcggaacca tcgttttgat ctactagtga tttggaaatt gacccgtctc
     4561 ggacgcaata tgttagatgt cttacaaaca gtcgaggaat tattgcgata tgacgtagga
     4621 ttacattcga tctcggaaca atttgacgtg accacttcga caggtaaatt gatgttacaa
     4681 ctccttggga gtttcggtga atttgaacgc aaccaaattg cggagaatgt tcaaatgtca
     4741 atgcaaagct tagtgaaaga taaaaagcgt tatgcaggag gccgacgttt aggctatgtc
     4801 agtggtattg acacgaatgg tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt
     4861 gttcgtctga tctatgcaaa atatttaggc ggagcagggt atcgagcgat tgccaatttt
     4921 ttgaatcgtc aaggatatca aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa
     4981 gatatcttac gcaataaaat ttatgcaggg tatttagaat atgcgcgcta catcaattgg
     5041 gaaaccaaac gacggaaggg gaagaatccg agaccgattt tagtgaaagg aacccatgaa
     5101 ccgattattt cggaagaaga ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa
     5161 caacccaaat ggaaccatac aggtgaaaat gtattgacag gcttgttacg ttgtccagaa
     5221 tgtggcgcac cgatggcagc gagtaatgtc acaaataccc taaaggatgg cacaaagaaa
     5281 cggattcgat attattcttg ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc
     5341 aattcgattc gtgccgaaca agcggaaaca tttgtcgccg accgattaaa ggagatcgtt
     5401 cagctaccag aagttcttcc ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa
     5461 agccaaccat tggaacaaga actcgtggtg ttactggaac gaaaggaaga attgaagact
     5521 aaaattgaaa aatgggaagc tgctttggaa gatagtcctg aattgtttcc gatgttaaaa
     5581 gaccgcttag acgaattgac tgaaaaacga cgacagttgc acattcgaga aaatgaaatc
     5641 ttagggatct ttcaacaagg agagccgatt caagtcaaag atgtccaacg gatattgact
     5701 agtttggatc gttttcttgc ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc
     5761 tttatagaga aaataacctt tgatcctaac cataaggaga tgttggaaat tacgatgaaa
     5821 ttcacgcatg ccgtggtaca acaattgaat gagcaatatc aaatagccgt atccaaaaca
     5881 aaggatacgg ctatttttgt gttaaaaaca ccctttacgt taacgatcta gtctaggtta
     5941 tatcaaacaa cgttagaggg gcgctaacgc ttctctagcg atttgaaatc gttagagaag
     6001 tagttaggga ggtccttatc ataaataggg atatttatta atgaatattt gaaatataat
     6061 tgtatataag aattaaaaag agtggggata gatatgaaaa agatgggtaa gtgtatttta
     6121 tgtgaaaaat acactgaact tactaaagaa catgtaccac ctaaaaaatc agggaatact
     6181 ggaggtaaaa agaccagaac tggtaattta gatgatttcc ttaagagcga ttttacaaaa
     6241 ggtgattttc cgaaggggat taaaaggaaa cctcaaggaa atgtttatta ta
//
LOCUS       DADVXO010000127         6210 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.131, whole genome shotgun sequence.
ACCESSION   DADVXO010000127 DADVXO010000000
VERSION     DADVXO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.131"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            281..637
                     /locus_tag="KB950_002399"
     CDS             281..637
                     /locus_tag="KB950_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6858576.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            647..1546
                     /locus_tag="KB950_002400"
     CDS             647..1546
                     /locus_tag="KB950_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBB6858577.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2063..2794
                     /locus_tag="KB950_002401"
     CDS             2063..2794
                     /locus_tag="KB950_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6858578.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2939..4966
                     /locus_tag="KB950_002402"
     CDS             2939..4966
                     /locus_tag="KB950_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBB6858579.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            5074..6171
                     /locus_tag="KB950_002403"
     CDS             5074..6171
                     /locus_tag="KB950_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBB6858580.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2153 a         1012 c         1276 g         1769 t
ORIGIN      
        1 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
       61 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
      121 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
      181 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
      241 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
      301 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
      361 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
      421 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
      481 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
      541 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
      601 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
      661 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
      721 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
      781 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
      841 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
      901 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
      961 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
     1021 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
     1081 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
     1141 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
     1201 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
     1261 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
     1321 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
     1381 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
     1441 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
     1501 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
     1561 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
     1621 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
     1681 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
     1741 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
     1801 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
     1861 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
     1921 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
     1981 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
     2041 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
     2101 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
     2161 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
     2221 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
     2281 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
     2341 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
     2401 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
     2461 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
     2521 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
     2581 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
     2641 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
     2701 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     2761 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     2821 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     2881 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     2941 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     3001 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     3061 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     3121 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     3181 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     3241 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     3301 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     3361 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     3421 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     3481 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     3541 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     3601 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     3661 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     3721 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     3781 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
     3841 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     3901 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     3961 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     4021 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     4081 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     4141 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     4201 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     4261 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     4321 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     4381 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     4441 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     4501 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     4561 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     4621 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     4681 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     4741 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     4801 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     4861 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
     4921 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     4981 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     5041 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     5101 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     5161 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     5221 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     5281 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     5341 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     5401 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     5461 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     5521 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     5581 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     5641 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     5701 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     5761 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     5821 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     5881 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     5941 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     6001 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     6061 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     6121 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     6181 aacaaatagt aaatttttat tattgtagga
//
LOCUS       DADVXO010000128         6141 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.132, whole genome shotgun sequence.
ACCESSION   DADVXO010000128 DADVXO010000000
VERSION     DADVXO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.132"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..172)
                     /locus_tag="KB950_002404"
     CDS             complement(<1..172)
                     /locus_tag="KB950_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HBB6858581.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKVSES
                     NYIEELIKVIKFF"
     gene            complement(226..2043)
                     /locus_tag="KB950_002405"
     CDS             complement(226..2043)
                     /locus_tag="KB950_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6858582.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2057..2821)
                     /locus_tag="KB950_002406"
     CDS             complement(2057..2821)
                     /locus_tag="KB950_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBB6858583.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2849..3547)
                     /locus_tag="KB950_002407"
     CDS             complement(2849..3547)
                     /locus_tag="KB950_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBB6858584.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3559..4338)
                     /locus_tag="KB950_002408"
     CDS             complement(3559..4338)
                     /locus_tag="KB950_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBB6858585.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4354..5298)
                     /locus_tag="KB950_002409"
     CDS             complement(4354..5298)
                     /locus_tag="KB950_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6858586.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5344..6123)
                     /locus_tag="KB950_002410"
     CDS             complement(5344..6123)
                     /locus_tag="KB950_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858587.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1585 a         1292 c         1116 g         2148 t
ORIGIN      
        1 caaaaaactt tattactttt attaattcct caatataatt actttctgaa actttaggta
       61 atactatttt ttcgtcacac atatatagtg cgggtgctag ttcagagcaa tctgctccaa
      121 tgatttttcc ttccatttca ttattgttta atgctgtttt aaatgcattc actagttcac
      181 aacgacgtcc acaacttaat attaaaatat tcaattttta atctcctatc tattcgaagc
      241 atttgcaaaa gtgacaatgt ctttagccaa ttgttcatcg tcttgcggca ggctcttgac
      301 aaaatccatc actttttgta ttgagtaacc tttgatgttt cctacaaaga ttttctcata
      361 aactgcttca tcatttcttt ccttatccaa caatagttct tcatatagtt tttcccctgg
      421 acggatacct gtttcaatga tttcgatttc gtcttcgcta tacccgctca atcggatcat
      481 gtttttcgct aggtctacaa tcttcactgg ttcactcata tctagaataa aaatttcgcc
      541 accttttgct aatgcgccag attgaataac taaacgacta gcttcaggaa tcgtcataaa
      601 gtatcgagtc atacgaaagt ctgttacagt gattggacca ccttgagcaa tttgttctct
      661 gaatactgga atcacactac cgcgagaacc aaggacattc ccaaatctga cagctgagaa
      721 tttcgtacag ccttcttcat tcaatccagt cacaatcatt tcagcgattc gttttgttga
      781 acccatgaca tttggaggat tgttagcttt atctgtcgat accatgacga agtttttcac
      841 ttttgctgct ttagctgctt ctgctacatt ttttgtaccg aaaatattgt ttttgactgc
      901 ttcttttgga ttatattcca tcagtggtac atgtttatga gcagctgcat ggtagacgat
      961 atctggatga tattgttcca tgatctcatt gattttctcg cgatcttgga tatctgcgat
     1021 gatcggtacg atttctgtag gacaattttg atgatgcgtg cgtaactcac ggtcaatcaa
     1081 gtagatcgag ttctccccat gtcctaaaag caatagcttt gcaggattga attgaatgat
     1141 ttgccgacag atttctgaac cgatcgatcc tcctgcgccg gtgacaagaa tgactttgtc
     1201 tgtgatctga tctttgatgg attcaatatc cagctttacc tcatctcttc ccagtaaatc
     1261 cactacgtca attgttttta attttgatac gctaattttc cctgaagcaa gttcttctat
     1321 agaaggcatc gtgttgactt tcacacgagc ggattcgact aactcgaaga ttcgtcgtaa
     1381 tttttttctt gataaggaag ggatagcgat cgttaccatt tgaatgtcat atttttcaat
     1441 caattccgga atatctttta atgcacctaa tacttttttt cctgaaagat acgtatttct
     1501 tttatttgga tcatcatcta caaacccgac aacgtggata tcttgagccg ttttggatcc
     1561 aagaaagctg ttgtataaaa tacgcccgcc ttcgccagca ccaacaatca atgtattttt
     1621 agcagaatct gctgatacgt atctcatatt ttttgtttcg atgtagatac gccagatcaa
     1681 tcggctaccg atgatcaata agagcgaaag cagataagca aagataacta accgtaaact
     1741 gtatcgccga ttaatgaaca gcagtatgag gatacttgag gcagcacttg cagatagtga
     1801 actaaaaatt gcaatgatct ctcttaaatt tgtatagcga ttgatccgag tgaaaacttt
     1861 gaataaacta ccataaaata aataaaaacc aatcgacaaa ccgatagaga ttaaaataag
     1921 atccattggt atagatacaa acggcttcat gaatttagct gctgcaatat ttgcagcaat
     1981 taataacaag ctgtccacaa aaaccaatgt ggcaattttt cttcttcttg ttaatataaa
     2041 cactttttaa ttccccctaa aataaaccaa acttcttctt ttttacctct tcatagtctg
     2101 gataactgat ttcatccccg ttgatcagat cctttgcaac ttgcttcatc tgtactactt
     2161 tttccttacc aaagtccttg gcaatctgct catagcactc ttttaagtaa aaggtgcgct
     2221 tagagactcc gtgtgcatca gaagcagtca tctgcacgag attatgtttg accattgttt
     2281 tagctgtttt ctgaatatct ttgccaaata ccccaacata acttggcgca gtaagttggg
     2341 ccaaacaccc catatctagg aacggaagca aatgattcgg gtcttcgcga aaatgcgcgt
     2401 tgcgttctgg atggacgatc actggtgtct ttcctagttc taacaactca aaaaaaagcc
     2461 gttctgcata taacggcact tccagtgtcg gaaattcgat cagaagatat gtatcatcta
     2521 aatctgtaaa taaaatttca tctcggtgga tgtcttcgat gagcgttccg gtaatacgta
     2581 cttcttgtcc ttccaataac gtcaaaggca actgtctttt ctctaattcg gcttgtaacg
     2641 aagcgactag agaaatgact tgtgattttt cattgctgta ttttccatta ttgtggtgtg
     2701 gtgtgcataa aatatgcgtg atgccttgct gaatcgcttt ttcagccatt gcaatacttg
     2761 cttctaagtt ttctgccccg tcatcgattc ctggtaggat atggcaatgt agatcgatca
     2821 tttctcttgt ctcctctctt cttattcctt aatttccgta gtaataacca gcatctttgc
     2881 tatgttctgc tccgttgtat acaacaccta aaactcgtgc ttgtaccatc tctagcagtt
     2941 cttttgcttt attcaatgat tctttacgtg atgtattttc acgtacgacc aataaagtgc
     3001 catccgtttt tgaagacatg atctgtgcat ctgttacggc tactactggt ggcatatcga
     3061 atatcaccac gtcaaataac tgtcttgctt cgtctaggat ctgatccatt cttggcgaac
     3121 ttaacaattc tgatgggttg ggtggcttag gtccactagg caaaatcgac aagttctcga
     3181 ctggcgttcg ttggattaca tctgctacac ttcctgatga actaagtgct gtacttaatc
     3241 ccctggcatt gttcagctgg aatgtcttat agacgacggg tttacgtaaa tctgcatcga
     3301 ctagtagaac tcgttgaccg gatttagcaa aaacgactgc aatgtttgct gcagtggtgg
     3361 attttccttc tccaggacct gatgaagtca cgacaatcgt tttgatttgc tgaccagctg
     3421 cggaggcaaa ctggatattc gtccggatcg tacgatattg ttcggcgatt ggtgaagatg
     3481 ggtttgttaa tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt ttctgtgttc
     3541 gtgccatttt tcttcctcct agactcttga gcggcttctg cgtgatgggg ctgttttatc
     3601 gtttgtagct tttggtcgtg caggagctgt tcctttttta cggataccag cgttcaattc
     3661 tttcgctgac atttctggaa ctgttcctaa gattgggaat cctaatgcat ccgtcacata
     3721 tttttcatct ttcactgtcc gatcaagtaa ttcgaataag aatgccagac ctacgcctac
     3781 catcattcct aatactaagc caatcactag attcaattta ttatttggcg aaacaggtgt
     3841 cgtattggca actgcacctg aaataatcga gattttatcg acattcatca cgtcttttgc
     3901 gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt tgagcagtca ctgcattcgt
     3961 gcttattgcc tggatcgaga acatttgtga attttgtgac tgattgacgg agatcgcatc
     4021 tttgatttgt ccagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc
     4081 atttagtact aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt
     4141 attgacatca ttgacatttg ctgtgtctga ttgtggcagt gtcacgatca attgtgcttg
     4201 tgaactgtat tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc
     4261 aaacattgag atgatgattg ttaccaagtg tttgcgtaat gtttcaaaaa tttctttcaa
     4321 gctgattgtc tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc
     4381 ttttaattct tttttcacac gatcaagttc ttgatcgctg actttttgat aggagatatt
     4441 atcttgcatg aagccttctc cttgcagctg atcttgcttg atcgtaccaa atgcctcccg
     4501 ataatctaaa gcgatttttt tcatttcatc aaagctcatg tctgttttca tattcgactc
     4561 tagagcattc aaaacggatt gatagttgct gacaccgttt aaactcaata ctttttttgc
     4621 tacaccttct acgatctttc tttgacgctc ttgtctacca tagtcaccat ttggatcttc
     4681 ataacgcata cgagaataag ataatgcctg atccccgttc aatgtaattt taccgattgg
     4741 aaagtcatag ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc
     4801 atcaacgagt tcctttagcc ctttcatatt gatagagaca tagtgatcga ttgggatgtc
     4861 tacatatttt tgtagggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt
     4921 gatcttgtct tttgtgtcac gacctacgat gtcgacatac gtatctcgag gaatgctgac
     4981 gatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc
     5041 ttgatcgacc cgacctaaat ctccagtatc aatgcccatc aatagaacag agaaagattc
     5101 ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta
     5161 agttttctca atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc
     5221 taatactaat acgataagaa ctgctaaaat aatcagaaat atttttttcc ctcttgattt
     5281 tcttcttcgt cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa
     5341 ctgtcaataa cttatattgc tatagcccat ttgctgagca acagtttgtg cttttgttgc
     5401 gtatgctgcg cttatttttt cattccggat ctgctctatt tgactgagcg ccgtcaaata
     5461 ttgatcgtaa gtagcggtgg cagtcacttg accatccttc aacatcgtat cgaataactg
     5521 atctagttgc gtgacttgcg cgatctgagt atcagcgaat cctagatatt gcataacaac
     5581 cgttttccaa ggactatctt taaaaaagct catattttca cgtacacgag cgacatctgc
     5641 acttgtcacc ttttctttga tgacgacatc gtctacagct tgctgccagt ttgatacatt
     5701 tttagtaaat aaatcgttga cagctgtctg gatcttcagt tttgattctg cactatctaa
     5761 ctgtgtcacg attgctttct tctcatcttg gattgcaaca gcttctttag gcaattctga
     5821 ttctttgatg tcgtaatcat ctgctgaaag tttgacagag gatagcttat ttgacaaact
     5881 tgtgattgtt tcttctgtta tttcagggtt tagaaaattt ttttcttttg tactatacag
     5941 cttgtctact tcttttgata aagaagtcaa ggtgtctttt tgattggtga tggcagattc
     6001 tgcataattg atccgttgct gatgttgccg ctgattattg atatggtaac caaacaaaca
     6061 gatcgcaata gcacctgcac ctaccgccac agcagtcaat gacttgacga atggcttttc
     6121 catagattca ctcctaagta t
//
LOCUS       DADVXO010000129         6129 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.133, whole genome shotgun sequence.
ACCESSION   DADVXO010000129 DADVXO010000000
VERSION     DADVXO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.133"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(62..547)
                     /locus_tag="KB950_002411"
     CDS             complement(62..547)
                     /locus_tag="KB950_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858588.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(686..901)
                     /locus_tag="KB950_002412"
     CDS             complement(686..901)
                     /locus_tag="KB950_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBB6858589.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1125..1925
                     /locus_tag="KB950_002413"
     CDS             1125..1925
                     /locus_tag="KB950_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6858590.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1944..3812
                     /locus_tag="KB950_002414"
     CDS             1944..3812
                     /locus_tag="KB950_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBB6858591.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3805..4296
                     /locus_tag="KB950_002415"
     CDS             3805..4296
                     /locus_tag="KB950_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBB6858592.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4284..4838
                     /gene="srlA"
                     /locus_tag="KB950_002416"
     CDS             4284..4838
                     /gene="srlA"
                     /locus_tag="KB950_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBB6858593.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4856..5851
                     /locus_tag="KB950_002417"
     CDS             4856..5851
                     /locus_tag="KB950_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBB6858594.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(5993..>6129)
                     /locus_tag="KB950_002418"
     CDS             complement(5993..>6129)
                     /locus_tag="KB950_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6858595.1"
                     /translation="KYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK
                     "
BASE COUNT         2117 a          980 c         1245 g         1787 t
ORIGIN      
        1 tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata atatcatcta
       61 atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt gacactcgat
      121 aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa gctgtgaaca
      181 aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt tgtaattcgg
      241 aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga agagaaggta
      301 cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata cttgattggc
      361 tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta gaagtacttt
      421 tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca gaattctttg
      481 ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg taaatccctt
      541 tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac ttaaacaaag
      601 aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc atgaagataa
      661 taactttttt aataattata gctttctaat atctttcatc aacttttcta gtaatcattg
      721 ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg atcttcgcaa
      781 aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac aagcctacca
      841 acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct aaagttttca
      901 ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga aacattgctt
      961 atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt tttgctcttc
     1021 cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt ttttagtgag
     1081 cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg gattggttgg
     1141 atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga agaagtatcg
     1201 tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact gaatgccgta
     1261 tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg gacatagaag
     1321 aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc aataatgcag
     1381 gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag tatgaattat
     1441 cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat ttgatgagcc
     1501 aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat ctttcttctg
     1561 aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa gcagcgatga
     1621 acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt gtggtaggtg
     1681 ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa gaagctcttt
     1741 cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga acaagtacga
     1801 tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc tattatcttt
     1861 cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt aaaacaagag
     1921 gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa ttattgaaat
     1981 tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc aaaaccagta
     2041 atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt attgcaaaaa
     2101 tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt tttgatgaga
     2161 tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa agaatcgctt
     2221 atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat ttgtctgaag
     2281 aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa gagatgatca
     2341 atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata ataggagatg
     2401 agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca gagacgaaaa
     2461 cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag acgaatgcta
     2521 gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat agaatctcta
     2581 gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa tatatccaag
     2641 aattcaatga actgatcaat cagttggaag aaatctatga actaactttg agccaatatg
     2701 aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg atgataagga
     2761 aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt cgacgattag
     2821 atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac ctctctttat
     2881 tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt gagatagaaa
     2941 aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta gaagctatct
     3001 tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg gagttagcat
     3061 ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat acgacaggaa
     3121 aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg gatatacaaa
     3181 taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat ttcgctgttt
     3241 ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat ttgactaatc
     3301 tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag cgattagccg
     3361 ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg tatcaagaat
     3421 atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa gaatttgaaa
     3481 aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa tttgcttttc
     3541 ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta ataactgatc
     3601 aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata cctgaagaaa
     3661 tgacggctga agttgagaat gaattatttg agctatacga tatcctattt gaactcataa
     3721 aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt ttaaaactaa
     3781 tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt tttcgcagct
     3841 tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt caatcaaaca
     3901 tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg gaaaatccgt
     3961 tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga agtcagaaaa
     4021 atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc gggtgaagat
     4081 atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt gacacaagca
     4141 gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga aaaaagtgaa
     4201 caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa aagtacgatc
     4261 aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag ggatttatga
     4321 ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc gtaccagttg
     4381 tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa gaccgagtaa
     4441 cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg ttaccgttct
     4501 tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc cttccagaat
     4561 actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc aatggtgtat
     4621 tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa gggatcgaaa
     4681 ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga ctgctgatga
     4741 actttattgg tggttggata actgatttta ctactggatt tgtatgcaga cagcaaggaa
     4801 tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt tttaaatgta
     4861 tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga ttattaggcc
     4921 gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc caatgatcgt
     4981 agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa catcaatacc
     5041 tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat gtggaattta
     5101 tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg gcccattagc
     5161 acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa tacagccact
     5221 aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata aaaagtacaa
     5281 atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact ggatagcaaa
     5341 aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa gagaagcagt
     5401 agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt tgattggtgt
     5461 gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt tagcaggaaa
     5521 tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat ctcctttgct
     5581 tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag agattgggaa
     5641 aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata ctcaaaatgc
     5701 atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga cagtagaagt
     5761 aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag ttatcattgc
     5821 ttgggtagca agttttggat tatatagctg agtttagtct aatggtgata agtcaagatg
     5881 aaatttaaga ttttctttat tgagtaatat gttttgaagc gcacgaaact agacccgatc
     5941 aaaatcaaat ttttttcggg ttgattcaag gatttgcgtc aataacggat tgttatttct
     6001 ctatcacact ccttggtggc gtatagagtt ggtgtttacg tagtagcgta tccaccagac
     6061 gcgtaaattt tcttgcggtt aggacgagtg cacgtttgtg ttgatgcttt ggaacctcct
     6121 tatatttac
//
LOCUS       DADVXO010000130         6066 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.134, whole genome shotgun sequence.
ACCESSION   DADVXO010000130 DADVXO010000000
VERSION     DADVXO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.134"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(254..541)
                     /locus_tag="KB950_002419"
     CDS             complement(254..541)
                     /locus_tag="KB950_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBB6858596.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="KB950_002420"
     CDS             complement(561..785)
                     /locus_tag="KB950_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBB6858597.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="KB950_002421"
     CDS             complement(1240..1410)
                     /locus_tag="KB950_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858598.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="KB950_002422"
     CDS             complement(1425..1958)
                     /locus_tag="KB950_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBB6858599.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="KB950_002423"
     CDS             complement(1979..2923)
                     /locus_tag="KB950_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBB6858600.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="KB950_002424"
     CDS             3560..3646
                     /locus_tag="KB950_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBB6858601.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="KB950_002425"
     CDS             complement(3767..4456)
                     /locus_tag="KB950_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858602.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4701..5615)
                     /locus_tag="KB950_002426"
     CDS             complement(4701..5615)
                     /locus_tag="KB950_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6858603.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="KB950_002427"
     CDS             complement(5597..5977)
                     /locus_tag="KB950_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBB6858604.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DADVXO010000131         6056 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.135, whole genome shotgun sequence.
ACCESSION   DADVXO010000131 DADVXO010000000
VERSION     DADVXO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.135"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1137
                     /locus_tag="KB950_002428"
     CDS             <1..1137
                     /locus_tag="KB950_002428"
                     /inference="COORDINATES: protein motif:HMM:NF016901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858605.1"
                     /translation="LRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDE
                     NHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEM
                     NKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDT
                     PDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAF
                     GKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIK
                     HEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYD
                     QTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1163..1603)
                     /locus_tag="KB950_002429"
     CDS             complement(1163..1603)
                     /locus_tag="KB950_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBB6858606.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1763..2269
                     /locus_tag="KB950_002430"
     CDS             1763..2269
                     /locus_tag="KB950_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6858607.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2437..4350
                     /locus_tag="KB950_002431"
     CDS             2437..4350
                     /locus_tag="KB950_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBB6858608.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4355..5356
                     /locus_tag="KB950_002432"
     CDS             4355..5356
                     /locus_tag="KB950_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6858609.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5558..5764)
                     /locus_tag="KB950_002433"
     CDS             complement(5558..5764)
                     /locus_tag="KB950_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBB6858610.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2235 a          926 c         1203 g         1692 t
ORIGIN      
        1 ttaagagaac ttaatactat tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt
       61 aatccgatag aaggagaaga ataccgtatc cgatactttt ttcacttgat gtattggcag
      121 tcattcgacg aaaatcattc tgatatatta tcaagtataa aaaataaacg tcaactatta
      181 gaaaaatttg cgaaacatgc gccatatacg cgtagtatag atagaaagaa atggatcagc
      241 ctactaagaa tttcgttgca acgaatgaaa caaggccact cgattacgta tgtcccagaa
      301 gagatgaaca aatttgtgca tccattgata acaaaagaag agtttacgca ttacatacta
      361 gagccttttt ttgaagcaaa cttctggtcg atgaatcaca tcactccaac agagcaagct
      421 tacctttatt ttatgtttgg tgtaatgaac acctatttgg aagaagattt gttggatact
      481 ccagataaag tacaagaaag ttggggctgg attgaatcgt ctattgtaga aaattttgaa
      541 aaaaacttca agatttgttt tacaacttca gaaaaaaatt atctatatgt caatttggca
      601 atgatccatt tgtattcaag ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa
      661 tcagcagaag acagagattt tcagcatata tttccagtga tgtatccaat catgcaacag
      721 ttttttagtg gggtagctcg cagaattcca gagcttaata cttattatga aaagaacaga
      781 cggttgattt ttcaatactg tatgttagtc agggatattt ttataaaaca cgaacaacct
      841 gtcatattct atattcaatc aaagtatggt aaaacacagg aattatgggc aaagaaaagg
      901 atattatcta tgactgaacg cccaataaaa tatagtgcat ccgctgatca gttacctgat
      961 attgtcatta gcgattatcc tatcgacaaa aaggcatatg atcaaacgaa tacacagata
     1021 ttttactgga atggacaacc tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata
     1081 aaaatcagga atgagaaaaa tagatccata ttggaaaaat tggatttcgt taattaagaa
     1141 aagcagattg tttatgccaa aattaagaca ttgcaaaaag attattttct ttaaaatcga
     1201 caagatgacc agaaagcaag agttcgttga gctcttgctt tcttttagtt ttagaaagga
     1261 acatcctgat tttatcttcg ctaaatccag tcaaaagttt tgcagattgg tcgtagataa
     1321 tcggtttttt caataaagaa ggggatgtac gaatctcaat atatagttca ctcaaactga
     1381 gttcatctat tctgtgaaca agatttttat aactaataga atgagtagag agcatttcgg
     1441 tcacgccgtt gtttgtcaat tttagtaagt ggataaattc atttttggtt agtggttgac
     1501 gaattaagtt ctgttcgata aacggtatat catggacgat aagccaacgt tttgcgcgtc
     1561 ttgaagacga gcagccagaa cctgtataca atttaatcat catacattcc tccaatagct
     1621 aagtatttat gaaataataa catgaagaaa tagtcgaaaa gtgccattta gaggtgtaaa
     1681 ataagaaaat gttgtcacgt ataatggaac tagctagata agaaatacta ttaaatcaag
     1741 aaaaaagaaa gaaggcacaa gtatgtcaaa cacagccgac gcaatagtag tgatcgattt
     1801 acaaaaaggg gtatgttttg attcttcgcc gatacacgaa ttagaaacag tgacagcatt
     1861 agtgaataaa agaatcaaaa catacgaaac gattgggaaa ccagttattt tcgttcaaca
     1921 cgaagatgct caattagtgc cacaaacaaa accatgggag ctattgtcgg aattatctat
     1981 tcctgagcac agttattttg tgagaaaaac ccatgcgaat tcattttata agactaattt
     2041 gagtgtttta ttaactgaat tggactgtca ttcgatcgaa ttatgtgggg cacaagcaga
     2101 gtattgtata gatacgactg tgaagtttgc tcatggatta ggataccagc ttatgatgtg
     2161 tagaggagca acgacaactt atgataatcc ttttatgaaa gcaaaggaaa caatcgcttt
     2221 ttacgagaaa atttgggata aacgatttct gacatttttg gagcaataag ttgtttcttc
     2281 gtttaaaaaa agcagaatgg atactggttt atccattctg ctttattacg taaaatgaga
     2341 aaataatcct gttctatttt tatgggataa accatttatt ttttgtataa aaaatgtttg
     2401 cgtttacata taattctcct agaaacataa aaaggagtga atagcttgag aacaaaacaa
     2461 gtattaaagg gagcgttggt catcttattt gctgtaagta caagcgtccc gcaatggaca
     2521 gtatatggag agagcatttc ttccgaatcg gaatctcggg aggtagaaac cacagaaact
     2581 actgatgctg gaaatgaaac gaaatcgagt acttcacaaa cagaaacaaa tactgaaaca
     2641 acggttgaga catctgttac tagcacagat caagaaacga ccgctaccac agataaccaa
     2701 caaacagaag aagcaacagc tacaaaaatg tcaaacgacg aatcctcttc tgctaaagta
     2761 agtgacaaag agaatgggac agaaaatgat gatatcggta aaggtgaaaa tttcaatggt
     2821 aaagaatatt tgaattactt cggtaaacag atgacagaaa tacttaatgg accaacagaa
     2881 gacatcaagt caaaattaat agagttatta gtcactgagc ttgctgttaa tgattatggt
     2941 gtagataaag agattatcaa acatctgatt gctacttatc tattgtacga tatcgctttt
     3001 gcaaaaaaca gtgtgttgat gtcgatgctt ggaggtacag atgatgggac aacgcctaca
     3061 gaaaacacag gcttctatcc aaatacaaaa caacttcatc aaactgtact aaacaaacaa
     3121 gatggcaaaa tgatggatgt atatgcatat tatgtcgatc aaggttcaga taaaacagtc
     3181 atgatccatg gcggatttcg agggaattgg aataacggga tcgttacaga agaatacaat
     3241 gatttttata aggccgggta taatttatta tttgttgatt ccagagcgac tggtaatagc
     3301 ggaggcgatt atgtgaccta tggacaatat gagtcagatg acgtccttta ctggataaat
     3361 caagaagttc gagagcggcc atctcaaaag attttacttt atggtggttc gatgggagca
     3421 gctacgatga tgtctgtatt agcgaaagat attcctgtca atgtcaaagg aattattgaa
     3481 aattgcggat ttgcatcaat tgatgaacaa ctacgtttta cgtacagcca gacagtagcc
     3541 cccgcactac cggatgccat aaaaaatcag ttagacatca ttggggatca agagcatgaa
     3601 gatttattta tgggcctttt gaaacaatac tattttgatc aggagatgca tttggataca
     3661 aaagctgctc tacctacgat tggaatgtca gattcattgc ctaaattgat tatccatgga
     3721 acagcagatg atgtagtacc tgtgtctaac gcgcaaaaat tatatgagtt atcaggaggg
     3781 tataaagatc ttttattagt agaaggagca gggcatggaa aggcacagga agttgaccat
     3841 gctgcctaca cgaaacatgt aacggacttt ttgaaagtag tgttccatga tcagataaac
     3901 gtaaagtatg tggatgaaaa caataaaagt ctactcaaag accaggacga aatccagctt
     3961 tacggagctt atggagaaaa ttatatgaca gagcaaaaga cattcgaagg atacgaatta
     4021 gcaaatgttg aaggaccgac tgaagggatt tttaatgaga cgataccaac tattattttc
     4081 tattacaaaa aaatcccagt agctccacca aaaaagcaag atccgacaga gaatgcagac
     4141 aataagggaa agaatgaacc tcgagaggat caatcaagtg atgatacgca accgattcag
     4201 atgacagtga aagtacaaaa aaagacagat gcaaaaaaaa gaatgttgcc gaaaactgga
     4261 gaaaagaaac attcgcttct catttattca ggtggatttt tatctgttag cacgattatc
     4321 acatggattt ggcgaagaaa gagagggtaa atgaatgaaa tggttcaagc gaatagtgat
     4381 tgcagtacca gttatcatcg tagttatttt agtcactgta tttttgatta atcaaatcac
     4441 accaaagccg attagcttgc ttgttagaaa acaatttgac acaagtgaaa aagaacaagt
     4501 atatgcccga ccagatgatt tccagcaaaa aatcgaacaa atcgatataa aaaaagaaat
     4561 atcctatcct tcaaagtaca aaaataataa tatggatatc tacacaccga aagtggaaaa
     4621 gaaaaaatta ccgattcttt tctggatgca tggaggcgca tatgtaggag gggataaaaa
     4681 tgattgtcgt gattatttgg aatatctatg ttcagacacg cagcaaatca tcgtaaatat
     4741 agattatgaa cgatcaccgg aagcaaaaca tcctacacca gttatccaat tgaacgaagc
     4801 cattcaagca gctaaaaaag agataaaaga tcaagcagat tggtcgaata tttcgattgg
     4861 tggaaattcg gcaggtgcac aaattagtgg agaatatttg ttagcacttc aaaatgaaga
     4921 aattaaaaaa gctgatcatc ttgatccgac tttggagaac aagcagatta cgaagtttat
     4981 ttctttgtct ggtctgttgg atccttctgc acttactcaa gtaagtgata aaatctctag
     5041 ctttctttat gaaaaatgtg gatgggctta ctttgataat aaggactttg aacaatcaga
     5101 gaaggtcaaa agtttagcct tggtcagaca tgcggatcgt tggacgcaaa acacattctt
     5161 aacggatgga aatacaaata cgtttacgaa acaaatggaa gaagtaactt ctgtatttga
     5221 gaaagcgggg gtgaaagtca caaaagtaga ttatgcggag aaaaatgctg ttttgaacca
     5281 tgaatatcag tttgatttca gcaataaaca agcgcaagaa acatatcaaa agttagtaat
     5341 gtttttcaag gagtaaagtc ttatgatgca aaagaggttg taatctgtca cttagctcca
     5401 agtcacaagg agcaatttca ataaacaagc ttttcatggt ttatgaatat tgtaacttgt
     5461 gactgaggag ttagagatgg caaggggcac cttttattcg aaatttggga atatcacacg
     5521 attattgtca cgatctcttt tacgtaaatt ttataagtca gatttgttgt tttgatcgtt
     5581 taaaaaaagc aacaaatccg aagcctgcgg aggcaagaag ggacaaaaag ccgagtggta
     5641 agcaaaagaa tgctggttcc actaatattc cgttgttatt gatataaggc tgggaacaaa
     5701 gatgaatagc aaataaaatc aatgcactag cgaaaaaaag caaagaaata tggatagctt
     5761 tcataagcag cacctccttt tttataattg tatgataaat agattaagaa tctataaatg
     5821 tgacaagttt gttaggatgt gaataaaatg atcatctagc gaacttgaaa caaagatggc
     5881 taatgataag caattaatga ttcagcaatc agataggtac aaagtttacc atcatacgtc
     5941 tagaccaata atagaaaata tgggaatata atagaaaaga tttaggatac ccccaaaaga
     6001 atttccccaa aaattcgatg tatcctaaac ttttttattt tacataacaa tcatat
//
LOCUS       DADVXO010000132         5868 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.136, whole genome shotgun sequence.
ACCESSION   DADVXO010000132 DADVXO010000000
VERSION     DADVXO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.136"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            36..1667
                     /locus_tag="KB950_002434"
     CDS             36..1667
                     /locus_tag="KB950_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB6858611.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1670..2749
                     /gene="uxuA"
                     /locus_tag="KB950_002435"
     CDS             1670..2749
                     /gene="uxuA"
                     /locus_tag="KB950_002435"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6858612.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2823..4250
                     /gene="uxaC"
                     /locus_tag="KB950_002436"
     CDS             2823..4250
                     /gene="uxaC"
                     /locus_tag="KB950_002436"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBB6858613.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4334..4981
                     /locus_tag="KB950_002437"
     CDS             4334..4981
                     /locus_tag="KB950_002437"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBB6858614.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5101..>5868
                     /locus_tag="KB950_002438"
     CDS             5101..>5868
                     /locus_tag="KB950_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6858615.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLL"
BASE COUNT         2001 a          802 c         1226 g         1839 t
ORIGIN      
        1 atcaaaacta acatatcagt ttgaggaggt taattatggt ttcgattaaa aattggcaaa
       61 attctatcga agaattcaat gaaatgcaga ttctactgcc gtgttttgat gtcaaagaag
      121 ttaagcaaga aggccagcaa catccagtat ggcttcattt tggtggagga aatctttatc
      181 gaggatttca tgcagaaatt gctcaatgct tagcagatca aaatgaatta gaaacaggag
      241 tagttgtgtg tgaaacattt gatgaagaag tagtggaaaa agcttatcat gactataaca
      301 atgacatttt ggaaattgtt atgagtggag atggtgaact caaaaaaaga cttttggcag
      361 taacggctga tagtttatat tgcaatccga agaatatagc ggactatcaa agagtggtta
      421 aatattttga agatcctaaa ttacagttta caacgtttac tattactgaa aaaggttaca
      481 gtttaaaaga ttctaaaggg caattctttt cggtagtcaa agctgatatc aaatctggac
      541 ctagcaatcc tcaacatacg atgagtattg taacatcctt gttatatagc agatttaatg
      601 ctggagagtt gccgattgcg atggtttcta ctgataattt ctcacagaat ggtcaacgtt
      661 ttcaagatag tatttttacg attgcagaag cttggcaaaa agaaggatat gtttctgaaa
      721 aatttattgc ttatttaaag gataaagaaa aagtgacttt tccttggagt atgattgatc
      781 gaattacgcc aaatccttca gagaatgtaa aggaagatct attgatgatg gggctcgagg
      841 atttagatat cattcacaca aaaaaacata ccaatattgc tgctttcact aatactgagg
      901 tagttcatta tttagttatt gaagatagtt ttccaaatgg ccggcctaaa ttggaaaagg
      961 ctggtgtgat tatgacagat agagagacgg ttgataaagc agacatcatg aaggttacga
     1021 caagcttgaa tccacttcat actgctttgg cagtaacagg ttgtctgcta ggatatcaat
     1081 caatttcagc agagatgcag gatgatgatt tggttggttt aatcaaagga ataggttatt
     1141 tagaaggttt accagtagtt gaaaatccag gcattattga tccaaaacaa tttattgatg
     1201 aagtaattaa taaacgtttg cctaatccct atattccaga tacaccacaa cgaattgcaa
     1261 cagatacttc tcaaaaagta gcgattcgct atggtgaaac aattaaaaag tatgtcgaaa
     1321 aagacggaaa tgcagaaaat ctaacattta ttcctatcgc tattgctgct tggttacgtt
     1381 atttactggc agtcgatgat caaggggaaa attttgtgcc aagtcctgat ccgttattgg
     1441 tagagttgca ggataaatta tcagtattga aattaggtga acaagatcct tcaaagattc
     1501 attgtgcggt acaggaaatt ttagaaaata aagaaatctt tggtttagat ttatatcaag
     1561 ttggggtagg aaagaaaact gaaaaaatct tttgcgaaat gctagtaggt cctcaagcag
     1621 ttcgtaaaac aatcaataaa tatataacag aagttggagg aaagtaacta tggaaatgac
     1681 ttttagatgg tatggggcaa atgaagaaaa aatctcacta caagaaattc gtcagatccc
     1741 agcaatgaaa ggaattgttg gaacattaat ggatatacca gccggagagg tttggccgag
     1801 agaacgaatt aaatctttaa aagatgaagt cgaagccaat ggacttacct tgaaagttat
     1861 tgagagtgtt aatgttcatg atgatatcaa aattggttta ccaacacgag atcaatacat
     1921 tgagaattat aaacagacga ttcgcaattt ggcagaattt gggattgagg ttatctgtta
     1981 taattttatg ccagtatttg attgggtaaa atcggatttg gattatcgtt tagatgatgg
     2041 gtcttctacg ttagcctata tttcaaaaaa tatccctact gatccggagg aaattattag
     2101 agaagtagaa aattctacta ataattttga attgccgggt tgggaacctc atcggttatc
     2161 acaaattaag caattattag aagcctataa agaagttgat gaagagaaat tacgagagaa
     2221 ctttgcatat tttctcaaag caattatccc gacatgtgaa gaagttggga ttaaaatggc
     2281 agttcaccca gatgatccac catattccat tttcggatta cctcgtattg ttaaaaatcg
     2341 tgacgattta gattggttgt gtaatgtagt ggattctcct tctaatggta ttacattatg
     2401 tactggaagt attgcagaac gaccagataa tgatgtctat gaaattctat ctgaattttg
     2461 tcaacgggat cggattcctt ttgcacatgt tcgtaatatc aaatatttaa gtgacaatgg
     2521 actcgatttt tacgaagcgc cgcataaatc agaatatggt tctctagata tgtataagat
     2581 attaaaaaca atgtacgata atggatttaa tggctatatt cgtccagatc atggtcggat
     2641 gatttgggga gaaaccggta gacctggtta tggattatat gaccgagctc taggtgcagt
     2701 ttatttaaat ggtatttggg aagcgcttga aaagggggcg agtaaataat tatataaggg
     2761 agtttattta gaggaaaaat tttctcttct aaaaactaac ggaaggaaag gagtgtttaa
     2821 atatgttttt agataagaac tttttattag agaatgaaat agctaaaaga ctttttcata
     2881 aatttgcaaa acaccaacca attatcgact atcattgtca tttagaccca gcatatttgc
     2941 tagaaaataa gcaatacaag aatttgactc aggtatggtt aaatgataaa ggagctggag
     3001 atcattataa atggcgattg atgcgagcta atggtataga agaagagttg atcagtggtc
     3061 caggtgatga ttatgaaaaa tttttagctt ttgtaaaaac agtggagaag gcgccaggta
     3121 atcctatttt tgaatggtct catttggaat tacaacgtta ctttgatatt gatttgacaa
     3181 tttctgagaa gaatgcacca gaaatttggc gacgtgctaa cgaaaaaatt caaacggatt
     3241 cctatcgccc gaaagaattg ttggagatga tggatgtgaa agccatctgt acaacggatg
     3301 atcctacaga tgacttgtgg tatcatactg agctttctaa acaggataat tctttcaagg
     3361 tattacctac tttccgtcca gataaggtct ggagtatcaa atcatcaggc ttttcgaact
     3421 atattttaaa acttagtcaa acagcggcta taccaatcaa agactttaat gggttgaagc
     3481 aagcattgag ccaacgagtg gatcattttc atcaaattgg ctgtcgatta gcagatcacg
     3541 gtttgaatac ttatttttat gaaatagcag atgaaaaaat actcgacaat ctgttaataa
     3601 aagctttagc aggaaaaacg gatttccatt tatcagaaat tgcacaattt gttacaggta
     3661 ttcaattaca tttgatgtct gagtatacaa gatatggttg gacgatgcaa atgcatttaa
     3721 atgctttaag aaataattca agtatattaa aggaacaaat tggagtagat gtcggtggtg
     3781 attcgatggg ggatcaagct agtttgacac aaaatgttgt agcattgttc gataccgctg
     3841 aaaagagtgg gtgcttacca aaaacaattt tatattcatt aaatccaact gattggcttc
     3901 cactagcaac agcaatgcaa agcttccaag ggggtatgat tcaaaaaatc caattgggat
     3961 gtgcttggtg gttcaatgat acagctgaag gaatgcgtca acagttgact acgatggcac
     4021 aacaaagttt gttgtcgaat tttacaggta tgttgactga ttctcgaagt tttctaagct
     4081 atcctcgcca tgagtatttt cgtcgagtat tatgtcaatt actaagcgag tgggttaaag
     4141 caggccgaat tccagatgat gaaatgtatc ttagtcagat agtggagaat atttgttatc
     4201 gtaatgcgta taattatttt aatttttttg aaatcggaag tagaaaataa tacttgaaat
     4261 aaaagcttaa atttattctt tgtaagttag tacagctaga caaagtatgt atccataaag
     4321 gaaggagata gatatgaaaa aagtagaaat tttaagtaag ttagaagcgg ctggagtcat
     4381 tgcggttgta cgtgggaaaa ataaggatga ggccattaaa gcaataaatt caattattgc
     4441 gggtggaatt aaagggattg aactaacttt tacagttcct aatgctgaag aaataatcaa
     4501 agaaattgct aaaaaatatg ctgatcaaaa agatgtaata atcggagccg gtacggtttt
     4561 agatgcgttt actgctcgta tcgcaattat ggctggggca gaatatatcg ttagtccatc
     4621 ttttgatcga caaacagctg agatttgtaa tttatatcaa attccttatt taccaggttg
     4681 tatgacaatt actgagataa aggaggcact tactgctggc gtagatatca ttaaactatt
     4741 cccaggttct gcttttggtc catccattgt aaaagcctat caagctccat taccacacgt
     4801 aaatataatg cctactggcg gagtaagtat agagaactta gaagattggt ttgaagtagg
     4861 agttgttgca gttggtgtag gtgggaattt attggcttct gttgaaaaag gtgattttga
     4921 agaagtaagt cgagtagcta gtaaatatat agataagttt tatgagataa agaattatta
     4981 ggagaaaaaa caaataatac tctgaaatgt aaaaaataaa tactgactct ttacgcttca
     5041 gagtatagta cgtactttca tcagaatgaa aacgcttaac aacagaaggg agagaattgt
     5101 atggaaagaa aatttggtat aagagacaaa ataggatata tgtttggaga ttttggaaac
     5161 gattttactt tcatttttgc tagttctttt ttaatgatct tttatacaaa agtattgggg
     5221 ataagcggag ctgccgttgg aacattattt ttagtagcaa gatttatcga tgcagttact
     5281 gatgtaacta tggggcgtat tgttgataga tcgaaagcaa ctaagcaggg taagtttaaa
     5341 ccgtggattt taagaatgag tggtccagtc gctttagcaa gttttttaat gtatcaaact
     5401 tctatgattt cagcttctat gagcgttaag gtagtataca tgtatgttac gtatatatta
     5461 tgggggagta ttttttacac atctataaat attccttatg gatcaatggc atcagctatt
     5521 acttctatta cagaagaaag aacaatgcta tcgacattcc gaaatgttgg agctacttta
     5581 gcaagtttgg tcattggtgt attaacacca ttatttattt atacaagaga tgattcaggc
     5641 gctcaaatag ttcgtggtgg atcaactttt acaattgttg cgggaatatt ttcgatatgt
     5701 gcacttattt gttatttaat atgttataaa ctaactactg aacgagtaag aattgaccaa
     5761 tctcaataca aagtttcatt gttaaaatct ttaaaagggt tagttaaaaa tagggcatta
     5821 ctttctataa ttggtgcggc tattttctta ctccttgctc aattatta
//
LOCUS       DADVXO010000133         5793 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.137, whole genome shotgun sequence.
ACCESSION   DADVXO010000133 DADVXO010000000
VERSION     DADVXO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.137"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(19..477)
                     /locus_tag="KB950_002439"
     CDS             complement(19..477)
                     /locus_tag="KB950_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6858616.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="KB950_002440"
     CDS             complement(490..1821)
                     /locus_tag="KB950_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBB6858617.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="KB950_002441"
     CDS             complement(1832..3274)
                     /locus_tag="KB950_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6858618.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="KB950_002442"
     CDS             complement(3261..4025)
                     /locus_tag="KB950_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBB6858619.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4129..5004)
                     /locus_tag="KB950_002443"
     CDS             complement(4129..5004)
                     /locus_tag="KB950_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6858620.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5259..5343)
                     /locus_tag="KB950_002444"
     tRNA            complement(5259..5343)
                     /locus_tag="KB950_002444"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5307..5309),aa:Leu,seq:caa)
     gene            complement(5361..5432)
                     /locus_tag="KB950_002445"
     tRNA            complement(5361..5432)
                     /locus_tag="KB950_002445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5398..5400),aa:Gln,seq:ttg)
     gene            complement(5442..5514)
                     /locus_tag="KB950_002446"
     tRNA            complement(5442..5514)
                     /locus_tag="KB950_002446"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5479..5481),aa:His,seq:gtg)
     gene            complement(5576..5660)
                     /locus_tag="KB950_002447"
     tRNA            complement(5576..5660)
                     /locus_tag="KB950_002447"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5624..5626),aa:Leu,seq:caa)
     gene            complement(5681..5751)
                     /locus_tag="KB950_002448"
     tRNA            complement(5681..5751)
                     /locus_tag="KB950_002448"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5717..5719),aa:Cys,seq:gca)
BASE COUNT         1755 a         1239 c          891 g         1908 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4081 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4141 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4201 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4261 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4321 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4381 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4441 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4501 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4561 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4621 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4681 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4741 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4801 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4861 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     4921 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     4981 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5041 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5101 taatttacaa aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt
     5161 ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat
     5221 agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc
     5281 ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5341 ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5401 agtcccttgc cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga
     5461 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat
     5521 aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg
     5581 gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct
     5641 gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa
     5701 ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta
     5761 ttatcaatct ataaaataaa gaataaatta tag
//
LOCUS       DADVXO010000134         5779 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.138, whole genome shotgun sequence.
ACCESSION   DADVXO010000134 DADVXO010000000
VERSION     DADVXO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.138"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..813
                     /locus_tag="KB950_002449"
     CDS             <1..813
                     /locus_tag="KB950_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBB6858621.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="KB950_002450"
     CDS             807..1229
                     /locus_tag="KB950_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858622.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="KB950_002451"
     CDS             2363..3331
                     /locus_tag="KB950_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBB6858623.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="KB950_002452"
     CDS             3341..3829
                     /locus_tag="KB950_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBB6858624.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="KB950_002453"
     CDS             4125..4406
                     /locus_tag="KB950_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858625.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="KB950_002454"
     CDS             4408..4824
                     /locus_tag="KB950_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858626.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="KB950_002455"
     CDS             5438..>5779
                     /locus_tag="KB950_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBB6858627.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2154 a          980 c          948 g         1697 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcggttggt
     5281 agaggggcta cttggcatag tgtacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DADVXO010000135         5767 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.139, whole genome shotgun sequence.
ACCESSION   DADVXO010000135 DADVXO010000000
VERSION     DADVXO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.139"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(450..953)
                     /locus_tag="KB950_002456"
     CDS             complement(450..953)
                     /locus_tag="KB950_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBB6858628.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1312..2337
                     /locus_tag="KB950_002457"
     CDS             1312..2337
                     /locus_tag="KB950_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6858629.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2660..3538
                     /locus_tag="KB950_002458"
                     /pseudo
     CDS             2660..3538
                     /locus_tag="KB950_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            4014..4130
                     /locus_tag="KB950_002459"
     CDS             4014..4130
                     /locus_tag="KB950_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBB6858630.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5058..5396
                     /locus_tag="KB950_002460"
     CDS             5058..5396
                     /locus_tag="KB950_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858631.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5490..5720
                     /locus_tag="KB950_002461"
     CDS             5490..5720
                     /locus_tag="KB950_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858632.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1959 a          867 c         1065 g         1876 t
ORIGIN      
        1 tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg
       61 atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca
      121 tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga
      181 gtagggtata aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt
      241 taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc
      301 ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa
      361 tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg
      421 tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat
      481 aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct
      541 aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct
      601 cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga
      661 atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt
      721 ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg
      781 gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca
      841 ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac
      901 cataatagaa agaataaata aatccctaat aaaaattttg atacataccg catagacatc
      961 tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt
     1021 ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt
     1081 ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa
     1141 aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact
     1201 aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg
     1261 catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag
     1321 acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta
     1381 cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt
     1441 tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg
     1501 aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg
     1561 acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc
     1621 gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc
     1681 atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt
     1741 atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct
     1801 gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc
     1861 attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg
     1921 aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac
     1981 ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt
     2041 gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact
     2101 tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt
     2161 cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg
     2221 atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg
     2281 gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag
     2341 gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag
     2401 gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac
     2461 tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg taactgaata
     2521 aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag
     2581 aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg
     2641 ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa
     2701 aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga
     2761 gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga
     2821 actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga
     2881 ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat
     2941 ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag
     3001 atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat
     3061 ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata
     3121 ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat
     3181 tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg
     3241 tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt
     3301 actatcaccc aatcttacta aatagttgaa aaaagagtca aaaatctgtc tttttgcagc
     3361 cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg
     3421 acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa
     3481 agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt
     3541 ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca
     3601 cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat
     3661 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
     3721 tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact
     3781 agctataaca atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac
     3841 gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt
     3901 taacaagttg actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt
     3961 cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa
     4021 aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac
     4081 gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa
     4141 acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt
     4201 gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag
     4261 aatctttctt ttcaaattga taccattccc taattccagt tatatagtta actacagaac
     4321 cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac
     4381 ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga
     4441 aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc
     4501 atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa
     4561 gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa
     4621 aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc
     4681 cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat
     4741 aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt
     4801 aataaagtat ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat
     4861 taatggttaa ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt
     4921 gatactagac tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat
     4981 gaaaaagaag ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta
     5041 gccaagtaac attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc
     5101 gtgctacaga agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta
     5161 gtgagccaat tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta
     5221 tggtggtaaa taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg
     5281 aaaaggtgag ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca
     5341 ttttaacgga ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac
     5401 tagtgaaata ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat
     5461 cgatcatcaa aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt
     5521 attacgtgaa tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc
     5581 gctactcaat taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa
     5641 ctgttttttg gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt
     5701 atgttagtat gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt
     5761 attgttt
//
LOCUS       DADVXO010000136         5696 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.140, whole genome shotgun sequence.
ACCESSION   DADVXO010000136 DADVXO010000000
VERSION     DADVXO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.140"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..434)
                     /locus_tag="KB950_002462"
     CDS             complement(<1..434)
                     /locus_tag="KB950_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBB6858633.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDA"
     gene            complement(447..671)
                     /locus_tag="KB950_002463"
     CDS             complement(447..671)
                     /locus_tag="KB950_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858634.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(775..2685)
                     /gene="ltrA"
                     /locus_tag="KB950_002464"
     CDS             complement(775..2685)
                     /gene="ltrA"
                     /locus_tag="KB950_002464"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBB6858635.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(3431..3568)
                     /locus_tag="KB950_002465"
     CDS             complement(3431..3568)
                     /locus_tag="KB950_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBB6858636.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(3565..4749)
                     /locus_tag="KB950_002466"
     CDS             complement(3565..4749)
                     /locus_tag="KB950_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBB6858637.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(5008..5298)
                     /locus_tag="KB950_002467"
     CDS             complement(5008..5298)
                     /locus_tag="KB950_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858638.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(5288..>5696)
                     /locus_tag="KB950_002468"
     CDS             complement(5288..>5696)
                     /locus_tag="KB950_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBB6858639.1"
                     /translation="RVYEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFP
                     NSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVN
                     SDSQLYKQAGNSVTVPVIFDIARRLKELDVHGL"
BASE COUNT         1524 a         1285 c          903 g         1984 t
ORIGIN      
        1 cacgcatcaa aatcaatata cgtcttcaag tgttctggta cttctccaaa atcaccagct
       61 tctacaatca agaaatatgc tacatcactc atatcatcgc aatctggata atagataata
      121 tcatccacat gctctaccat ctcctcaaaa gaattgaaga aagcatgttg aatctcagaa
      181 gcaacttcac caactggcgt tccttcaagt tcctccgcta agccacacaa ccgattaatt
      241 tcctcaattg gcgtatactc atcaatctca aagggaagtt cataatcatg aattgcatat
      301 tcctcgtatt cgtcatttaa cccaattcgt tctttcactt catcaaaatc gacaggtggg
      361 gtaaaccacg cccccaccaa ttcaccttcg ttatatttcc cgaggttcgc aatataaaca
      421 cgcatctgtt ccatcgtgct tcccccttac gcaccgacaa ttcggttaaa caaactgagt
      481 aagacatcct tcaccccaga agcattaaag accaatccga ccgcaatcaa agcaatcacc
      541 aaaaagccaa tcagtttgga aaactcccgc ttaaatccta aaaacaaacc aatgaccaca
      601 attgccagta agactaagga ttgggcattg gttaaaaacc attgatatat ggataggtaa
      661 gttcctgata ttataaatat ctcaaacttt tccccccatt cacacggagc gtgcgacttt
      721 caccgcactc cgcgttccat cgtgtttata ttgaaaagta tttatgtagt agacctatat
      781 caagtttaat tcttgattgc cagctaatac tctatattta tttagtctta tgagctggac
      841 tttatttagc ttcaatagag ctatatattt ttgtattgtt tcttctgttt tagcgtgtat
      901 tagacggtgt accgcctctt ttaccaaaat aagattttta taatcgtcat ttccaccagc
      961 tccagttggt acaatatgat gacaatgtat ttcattagct tgttgaaatt cttccagagt
     1021 tatagcacat tttccatgtt gagcagaaaa tagcgaaatt cgattgtctg cgtattctat
     1081 tgtgcgattt tccaaagata atttcattaa ctggtacatc agtaatttat caacacttag
     1141 attggtgtgg ataaattttc ttccctctag agtgtagcaa ttcgctaaat acacctttgc
     1201 cattggtttt ctgtgttgga tatatccaac agggaaaata ggttcgccct cgccagcaac
     1261 ataccgaagc attttagatt taccatatct tgttgcctcc tttgtcgtaa gttgtctgcc
     1321 tgtttttctc aatctgcccc ttttgtttgt acctaatctg ttagtgaaga tagccattac
     1381 agctctattc agtttcatac aatccagatt tacattcgtt gcaatctgat aataattttg
     1441 catacctaat accattgaat tataaagtct tgtttcttct aattccgttt tcccttttga
     1501 gggagaagca attttctttg cctgtgatac cagcttgtct ttttggtttt tgaattgttt
     1561 atcacttata tgtgatttaa caactaactt ctttcttctc ggtatcattt taatcttgaa
     1621 accaagaaag tcggagtatc tttttctaac atttacaatt cttgtttttt cttgtgatac
     1681 ttccaatttt aatcgttgtt caatccattg agtgattgct attttggctt tttcagcact
     1741 ttccttgtag cggcagaaaa ctctaaaatc atctgcataa cgaaccatat acatttcctt
     1801 taactttgtt ttcttcattt ctttgtaagc gttgctttta catgggctac cgcttttatt
     1861 gatatgaacc acatagtttt ttgtaacagg attttcctgc cattggcttt ctacccaatg
     1921 gtctaactca ttaagaacaa tgtttgctag tagtggggat attattcccc cttgcggagt
     1981 tcctttctct ggatatgtta ttgtaccgtt agtcattttg attggagctt ttaatatcct
     2041 ttttaaaatg aatatcaagt gcttgtcccg tattcccata gcccatatct gttttatcag
     2101 cttggaatgg ttcacgttgt caaagaaacc cttaatatca aattcaatca cataatgtag
     2161 ctggctgatt tgtaggcggt tataggttgc ttttattgca ttttcaactg acctattagg
     2221 tctaaaacca tagctgtttt cactaaattt agcttcgcag attggttcta atacttgttt
     2281 tatacattgt tgtataagcc tgtcccacat acaaggaata cctagaggtc tggttttacc
     2341 atttggtttt ggtatttctt ttcgcctcac tggcttaggt cgatacccat gtggactgcc
     2401 tgcaacaatg tatcttactt ttttgacaac tgtttctgct ggcaatttgc caatatcttt
     2461 aattattgta ccgtctgttc ctgctgtttt actgcctgca tttgctttga tatttcgata
     2521 tgccagtaat atattttctc tcgataggat aatatccata agatgagtga aaatatctcc
     2581 attcttactt ttggcatata aatcatcaaa ggtgttttgc atttcgtaat attcagcgtg
     2641 tcttaaatct tctacacaca atgtttcatt tttattttta ggcataaggc ttcactcctt
     2701 tacttgaaat tgagtgagct tttttatctt actcgtaatc cttattgaaa caacataata
     2761 cttttctaat atatacacga cttgagggca ttcctccatt ctcattacaa gaatttctta
     2821 ggttcgccct ctgcttttca gcattagttc aactgcttat tgttcttcgt cagtttattc
     2881 tcttgatgag aacccaatac ctttcctcgt tccgataatc ttatctatac atatatatcc
     2941 ttaggtcact agctataagc ctgctggctt gataccgcct gtaacgttat atggcatttc
     3001 ataacctata ttcttacttt gccacaccag ctacgatttc tcgtaccttt gcatttctac
     3061 aaagttcaaa tttagacccg tacattcgct gtttcgtcag ttccttatag aacattctaa
     3121 ccatagatat tttatagacc cccgacctgt tggatacgct acccacctct ggatagtttt
     3181 cgatagaaga tatatgttca tcaactatta cggtatcttt cagccgtctt taccgagctt
     3241 caaacattct gagtttcctc atacaatgct tgtcggaggc tcagggcagg cgtttcagag
     3301 cgttacttca tcattccacc tgtcagatta tcagttctcc taataaccaa actttttcgt
     3361 tcttttatta gtgccttagc ttttcactaa ggaacgagtc gcacataaat tttgtccgaa
     3421 attcatcttg tcactttcct ttcttttcca ttgctcgatc cgctgctgca cttgcattca
     3481 tcaagcacat cacagtaaca ccaagcatac tgccaccaaa taccaaaaaa atatctttta
     3541 caattcccat gacgattcct cttttcatat aatcaaattt tcaattgcca tactttgttg
     3601 ctccaaaatc tttttatgtc ggtctgataa gcgtgcattc tgtaccatat cctggatata
     3661 ggttgtccga ttcaatcgat ccaactcctt agccattttc cacgtagggg cgacttgtcg
     3721 gcctagccaa ttcaaggttc gttcaatggt atacggtttt ggctcggaag ttaattgaat
     3781 ctctttccga ttccgtccaa taaaacgccc ccattgttca ttcaagggcc agttggttcg
     3841 ccttttacct tctactttat ccgcaaatcg caagtaacga ttgataatgg aaaacgtggt
     3901 gctctcaatg ctacgacctt ttaacaaatc ttgaatcgca tgaaaagcac ggtcattttt
     3961 cagtcgaatc tcaaaacgat ttttcgtctc agtttggtct aatggaatcc ctaacttgat
     4021 atattgttca tagtcttttt catacaaaca gaaataaacc tcactcttga gactgccgat
     4081 atataaggta ttgcccatgc cgtccttttc atcagccttt aaaagttccc cggaacgata
     4141 acttttaaag gtacgaaaca aggagataca ttcttccttc tgacattttt tcgttagctc
     4201 aggtatatcc aatagcccca ctcgatcatt gattgccaag tccaagcgtt tcatcacgcc
     4261 accggccttt agacaatcct cgaaaaagtc gtaccagcta cgcttttgag cgagtaaaaa
     4321 agtttcaaat tgtcggcaac ctcgcccctt taattctaaa agcacacctt tatcttcttc
     4381 atgagacaac atcaccacaa tatcacccat gacatactgt tcttgatagg agtaaaaggc
     4441 ataatcttca tggagcatat actcaaattt caaatggaga atttcttgaa taatttttcg
     4501 tgcatccgtt gtgggaaagc gaatccttac atagtcaaac agtaacgtca aaggcaaatc
     4561 gggattaaag ctttccagtt ggtcaaaaat tttcctcatg gtttttatag atgcggttac
     4621 gccaccattt tctatctgat ttaagtacgg ccgactaagt ccacaagccg tagccaattt
     4681 attttgtgaa acgccgtatt cgttgcgtcg gtcttttaat agccgataat ctaaattccg
     4741 ttgtgccaag ttctcacctc caaattcaag gtgttaactt tttcaaatga gttaacacct
     4801 atacaaatta cttactatct ttgatacaac aagcattctt aatggttgct tacttatttt
     4861 ttgtgttttt gtacccccct gttagatacg ggggtgagac atcatggtcg gctaacgccg
     4921 tccatgaaca ggcacagcac cacggaggat ttctgcttcg cgaatcctcc gcggtcagcg
     4981 cctaacagtc agttgtgttg gtttaggtta ctcatctgta ataccattta cttccaactc
     5041 tgctttcatt tctaacaagt attgacgaat atctggttct tttgcttggt ccgcaatccg
     5101 ctcaaattct gcaattactt gatttatttc aattcgagca tccgtctcct cattcattaa
     5161 atatggatcg ccagcgactt tgacaccatt cttgcgaata tcaaatttta accgtcgcaa
     5221 ttgttcttct aattctgggt ctgactgaat ctttccaatc acccaataaa tttctccaat
     5281 caactcatca taaaccatgt acatccaact cttttaatct tctggcaata tcaaaaatga
     5341 ccggaactgt tacagagttt ccggcttgct tatacaactg actatctgaa ttgacttgag
     5401 aatctcgatc aaaagcccaa tcgggaaagc cctgaagtcg ccaacactct ctaggtgtta
     5461 gcttccgaat ttgatactga tccgtgacga tcgcttgttg gtctcctgtt agtaaggtat
     5521 gaacactctg ttttcccaga cgacctcggc gacttgtgga attaggaaaa gccagattca
     5581 cactgtcttc tggcttcacc tcttgatatt ctgtttcatt ttttcgaatc atgatttttg
     5641 taccaccttg tttcgttgac aaggtggggg cggttccagt aacttcataa actcga
//
LOCUS       DADVXO010000137         5652 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.141, whole genome shotgun sequence.
ACCESSION   DADVXO010000137 DADVXO010000000
VERSION     DADVXO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.141"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(29..1261)
                     /locus_tag="KB950_002469"
     CDS             complement(29..1261)
                     /locus_tag="KB950_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBB6858640.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="KB950_002470"
     CDS             1516..2085
                     /locus_tag="KB950_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6858641.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="KB950_002471"
     CDS             complement(2263..2703)
                     /locus_tag="KB950_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBB6858642.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="KB950_002472"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="KB950_002472"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBB6858643.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="KB950_002473"
     CDS             3657..4580
                     /locus_tag="KB950_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBB6858644.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="KB950_002474"
     CDS             complement(4805..5044)
                     /locus_tag="KB950_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858645.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="KB950_002475"
     CDS             complement(5049..5468)
                     /locus_tag="KB950_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6858646.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
BASE COUNT         1732 a         1067 c         1045 g         1808 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tt
//
LOCUS       DADVXO010000138         5593 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.143, whole genome shotgun sequence.
ACCESSION   DADVXO010000138 DADVXO010000000
VERSION     DADVXO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.143"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            442..999
                     /locus_tag="KB950_002476"
                     /pseudo
     CDS             442..999
                     /locus_tag="KB950_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1057..3096
                     /locus_tag="KB950_002477"
     CDS             1057..3096
                     /locus_tag="KB950_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBB6858647.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3205..4356)
                     /locus_tag="KB950_002478"
     CDS             complement(3205..4356)
                     /locus_tag="KB950_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBB6858648.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4510..5040)
                     /locus_tag="KB950_002479"
     CDS             complement(4510..5040)
                     /locus_tag="KB950_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBB6858649.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5139..5489)
                     /locus_tag="KB950_002480"
     CDS             complement(5139..5489)
                     /locus_tag="KB950_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBB6858650.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2026 a         1025 c         1043 g         1499 t
ORIGIN      
        1 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
       61 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      121 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      181 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      241 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      301 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      361 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      421 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      481 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      541 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      601 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      661 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      721 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      781 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      841 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      901 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
      961 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1021 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1081 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1141 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1201 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1261 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga tcaataccag
     1321 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1381 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1441 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1501 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1561 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1621 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1681 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1741 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1801 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1861 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1921 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     1981 aaggatcttc gtgggacaac aggtgagaaa ttaagcatta cagatgcaga cggtgtagag
     2041 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2101 gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc aactgttgcg
     2161 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2221 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2281 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aataattaca
     2341 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2401 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2461 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2521 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2581 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2641 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2701 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2761 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2821 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2881 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     2941 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3001 atgatggtca gcatgttgga aaataaagat gatgatgatt cagcaaccaa acgagcatcg
     3061 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3121 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3181 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3241 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3301 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3361 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3421 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3481 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3541 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3601 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3661 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3721 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3781 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3841 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3901 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     3961 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4021 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4081 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4141 actttccaga ccagctaaaa acattaataa gaacacacca atctccgaaa agtcatggac
     4201 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4261 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4321 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4381 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4441 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4501 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4561 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4621 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4681 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4741 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4801 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4861 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4921 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     4981 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5041 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5101 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5161 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5221 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5281 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5341 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5401 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5461 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5521 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     5581 gattgtgaca aaa
//
LOCUS       DADVXO010000139         5389 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.144, whole genome shotgun sequence.
ACCESSION   DADVXO010000139 DADVXO010000000
VERSION     DADVXO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.144"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            79..870
                     /locus_tag="KB950_002481"
     CDS             79..870
                     /locus_tag="KB950_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6858651.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            836..1789
                     /locus_tag="KB950_002482"
     CDS             836..1789
                     /locus_tag="KB950_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBB6858652.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2187..3161)
                     /locus_tag="KB950_002483"
     CDS             complement(2187..3161)
                     /locus_tag="KB950_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6858653.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3276..3710
                     /locus_tag="KB950_002484"
     CDS             3276..3710
                     /locus_tag="KB950_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6858654.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3835..4857)
                     /locus_tag="KB950_002485"
     CDS             complement(3835..4857)
                     /locus_tag="KB950_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBB6858655.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5249..>5389
                     /locus_tag="KB950_002486"
     CDS             5249..>5389
                     /locus_tag="KB950_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBB6858656.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRA"
BASE COUNT         1710 a         1024 c          958 g         1697 t
ORIGIN      
        1 aataaaatcg tttatctata taattaagaa gaacaaagtc gaacaaaaat tgacttttat
       61 gttaaaggag taattcatat gctgaaaaaa gaaagacaag aacaaatcat tacattatta
      121 gagaaagatt tttatttaac taattcagaa atcgctaaca aattaggcgt atcagaaatg
      181 accatacgcc gagatgtcac tgaattagca aaaaaaaata aagtaaaaaa gctatatggt
      241 ggcgttgaac gaaaggaacc ttataatcga gaactatcga ctcaagaaaa gattgatacg
      301 aatgtagccg aaaaaaaata tattggtcaa gtcatgaaca gtatgattcc cgataattct
      361 actatttatg tcggtgctgg aacaacgatc ctatatgcac ttcctttcat tcaaaagaaa
      421 aatctatttg taattacaaa cagtttgatt gctttcaact atttaaagaa tcatacaagt
      481 tatcggattt tacttactgg tggcgaattt tctcctatta cagaagagtt cattggggaa
      541 gttgcagaaa gatcttttga aagtttgaat gtcgatatct ctttcgctgc aacaaacggc
      601 atatttgata acaatgtgac aaccgctcaa tttattgagg gaaatattca gaacgcagcg
      661 ctcaatgcag caaaaatcaa atgcattgtt gcagattatt caaaattaaa taaatcagac
      721 gtctatactt ttagaaaatt aaccgatttt gattatctaa taaccgataa tcatttatca
      781 gaacaggatt tcaatcatta ttcaaaatac ttaacaatat taaaggagcc caaaaatgat
      841 actgacaatc acgatgaacc catccattga ttccatttat tttacaaaac actttgaatt
      901 aggtacgatg acacgctttg attctccggt acgctatgta ggcggcaagg gaatcaattg
      961 cggacgagct tctgctctac taggttcatc agttatttta acagggtttt tgggcggtta
     1021 ttttggcagt atgattcaag atatgctgaa aaatgaaaat ttgtttgatt tgcatttttt
     1081 acctattgca gaagaatcaa gaggtgccgt cacgatcatg catgatgatg aaacgcagac
     1141 agagctagtg gaagctggtc ctgaaatagc agatactact atcaaaaaat taatgaatga
     1201 tatcaaacta atcagtaaaa ataattacct ttcagtcatt acaataaatg gttctgttaa
     1261 caatccagat ccctttttgt atgctaaatt attacaatat atcaaagaac aaatagatcc
     1321 ttccacctat atattaatgg atatatctgg acaacatctc cgtgaagtct tagcaagtaa
     1381 gtataaaccg atgttcatca aacctaatat ccatgagttt tcagatatca tccatcaaaa
     1441 aattactcaa aaaagcgaca taaaacacca tctaaaaaca aatccactat ttaaagatat
     1501 cgatgttatc atgatttctt gcggctcgta tggtgcagta gtcaaagtaa aagaagaatt
     1561 ttggaatgtt gagatcccta agattcctat cattaataca acaggctctg gggatgccac
     1621 tgttggaggt tttgctcatt cattagaaca gaattatcct ttaaaagaat gtgtaaggta
     1681 cgcaatggct tgcggcatgt cgaatgcaca gcaatcagaa gtcgggaaag ttattccagc
     1741 tgatgtaaaa aatttagcaa aaagcattaa aatcacagaa atgaaataaa tagtacaaag
     1801 taaataattg tttatatgaa atgagacaca aagagactgt gacaaaaaac gtgtgtaccg
     1861 acccccaaaa gttagatttt ttggtctaac ttttgggagt cgatacattg tcacagtcct
     1921 tttttcgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atattcaaca
     1981 aacatgagaa gctatttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca
     2041 cgacctcttg gtataaacgg tgttcaaaag cttgcatcta cgctaatgag tccaaccaag
     2101 tcaaaaatga ggagcttttt tggctgtcct tattacttga tgcagggcag ttttttcata
     2161 atctctttta gattaaatcc ttttggttac acaaggttct cttcaaagaa tgttgccatt
     2221 ttttcaaatg gaatgatgtc tgtctggtcg tatagatctg tatgggatgc tcctggaatg
     2281 atcatgagtt ccttgttgtc accttttagt tctttgaaag cgtcctcccc aaagtaacgt
     2341 gaatgagctt tttccccgtg aatgactaag actgcatgac ggatttctgg aatataatgc
     2401 aatagttttg cattcataag tgacaaccct gatgtcctat tccaaccatc attggagttt
     2461 aaagaacgga catgatatcc gcggtctgtt ttataatacg catgatagtc tttgacaaat
     2521 tgaggagcat cttcaggcaa cggatcaacc acacctccag cacgtgcgta gtccccttgt
     2581 ttgaaatcaa tgatgcgttg tgcatttaat tcttcacgca tttcatatcg ggcattttca
     2641 tccataacat cgaagtagcc atttgctgtt actcgagtca tgtcatacat tgtggaaaca
     2701 actgttgctt tgacccgcgt atcgatagct gctgcattca atgccatacc tccccatcca
     2761 caaataccaa tgattccgat tctctcaggg tttacatttt tttgtacaga taaaaagtct
     2821 actgccgctt ggaaatcttc cgtattgata tcaggcgaag caacatatcg aggtactcct
     2881 ccgctttctc ctgtaaacga tggatcaaac gcaatcgtta aaaagcctct ttcagccatc
     2941 gtctgcgcat atagaccaga cgattgttcc ttcactgcac caaatggccc gctcacagcg
     3001 attgccggca atttcttttc acttacttct tttggttcat ataaatcagc gactaatgtg
     3061 ataccaaaac gattcgggaa gctcaccttt cgatgattga cttgattgct ttttggaaat
     3121 actttgtccc attcttgtgt gatgtttaat tctttattca tattctagac tctcctttga
     3181 tcaaaattga catttgtttt tgaatgtatt attatgataa aacttaaact tgactttagg
     3241 tcaaggtaaa aattttattt taggaggatt tttttatgta ttccattgga caagtgtctg
     3301 aaatgtttgg attgcctgtt tctactttac gttattacga caaagaggga ttatttcctg
     3361 atatccagcg tgtttctggt attagaaaat tttcagagaa tgagttagag gctttaagag
     3421 tcattgactg tttgaaaaaa tctggtttag aaataaaaga cattaagcaa ttcatggaat
     3481 ggagcaaaga aggcagtaag acatttgagg ttcgtaaaga actatttgaa aaacaaaaag
     3541 aagtagtaga aaaagaaata gcaaaattag aaagagtact cgctatgcta aactacaaaa
     3601 gctggtacta tgaagaagca ataaaagccg gcaatgaaga agccgtattg acaatgattc
     3661 ctgatgattt accccaacat gtaaaagaag cttatgtaca ttctcattga acaaacggat
     3721 aactcactct ccttctctat gaacttcatg aaaacatgaa taaacacctc taactgattg
     3781 ttagaagtgt ttattttttc actcaagtat cctgcactta caaaaatatc ctttttaccg
     3841 ccatcctaat tctggagcga tatcttttac gattgtttca agcaagtgca tgttatattc
     3901 tacccccaaa gtattaggaa tcgtgacaag tactgtgtct gcttctttga ttgcttcatc
     3961 ttcagctagc tccttgatta attgatcagg ttcaccagca aaagtttttc caaaaatcgt
     4021 tgggtttcgg tgataagcaa gatagccgac agcatcttgt tgcggactac cgtcttgtcc
     4081 aaataagcgt cgatccaaat cagtggtgat tggctgaatc gagcgagtaa ctaatgttct
     4141 tggttcaaaa tcatgacctg cttgcttcca cgcttcttta tacgcacgta attggtttgc
     4201 ttgttggata tggaatggct cgtgggattc gtaattgact aatgtagatg attgcagatt
     4261 cattccatgt tgtgctgccc agattgccgt ttgctgcgaa ccagctcccc accagattct
     4321 ttgtcgtaat ccttctgaat aaggttctat ccgtaatttt cctgatcctt gtgggaacat
     4381 tggttgagga tttggttctt caaatggttt tccttcaagt aattccaaga attcaagggt
     4441 gcgttcccgt gttacctctt tgatttcttc ttcactgtaa tcataaccaa agtatttcca
     4501 gccatctaga acttgttcag gtgaaccacg ccccactcct agttgcactc ttccttgcgt
     4561 gatgatatct gtcaatccgg cattttctgc catataataa gggttttcat atcgcatatc
     4621 aataagtcct gtaccgattt caatcttact tgtctttgcg ccgattgccg ctagcaaagg
     4681 aaacggcgac ccaatctgag gggcaaaatg atgtacgcgg aaaaacgctc cgtccactcc
     4741 gattttttct gcttccaccg ctaaatcaat cgattgcaaa taagcatctt gagctgattt
     4801 cacaagcgat ccttgctcca tccaatgtcc aaagcttaaa aagccgattt ttttcaaggt
     4861 aatgacctcc tttaaaccta caaaaatgga aggtttttta tttttaatat gaactgattc
     4921 tactactttt tttcttaaaa gacgaataat tgttctcgcc tcacaatcaa gaaatgtatc
     4981 ggcttactac acccctactt tcttcatcat ctgccgtgtc ttaatctctt ttaacagtct
     5041 gttacatcgt tgatttttga ctcaaaatga ttgtttctaa catgtattta tggtaaaaaa
     5101 gacaagatta gttctttgta cacatcgatt tatacgctga acaaaaagaa atatataaac
     5161 taagaaaccc attgtttttt cgctccatca catagagttt actttagata aacagtgaca
     5221 tttactaatc cataacggag gaaatcaaat gaaaaaaatc tatttcttat gtacgggcaa
     5281 ttcatgccgc agtcaaatag cagaaggcta cgcgcgtaaa tacttgtctc cttcaatgtt
     5341 tgagataaga agtgcgggga tcgagacaca tggcttgaat ccaagagct
//
LOCUS       DADVXO010000140         5365 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.145, whole genome shotgun sequence.
ACCESSION   DADVXO010000140 DADVXO010000000
VERSION     DADVXO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.145"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..281)
                     /locus_tag="KB950_002487"
     CDS             complement(<1..281)
                     /locus_tag="KB950_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858657.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGT"
     gene            complement(579..1553)
                     /gene="sgrA"
                     /locus_tag="KB950_002488"
     CDS             complement(579..1553)
                     /gene="sgrA"
                     /locus_tag="KB950_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBB6858658.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1749..2588)
                     /locus_tag="KB950_002489"
     CDS             complement(1749..2588)
                     /locus_tag="KB950_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6858659.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2773..3660
                     /locus_tag="KB950_002490"
     CDS             2773..3660
                     /locus_tag="KB950_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB6858660.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4254..4948)
                     /locus_tag="KB950_002491"
                     /pseudo
     CDS             complement(4254..4948)
                     /locus_tag="KB950_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(4932..5330)
                     /locus_tag="KB950_002492"
     CDS             complement(4932..5330)
                     /locus_tag="KB950_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBB6858661.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1709 a         1083 c          785 g         1788 t
ORIGIN      
        1 atcgttccct ctaaaagtat agaataaact ttactcagta aaaactgaga agaaaactcg
       61 acttttatca tcttgttttc tgaaagaaaa gataatttat attctggata cctttcgcaa
      121 aacgattctg cttgttgaat caccgaattc atcttagcca gggtgtagtt tagatctaaa
      181 gcgatatcct tcaccacata taatttctcg tctctttcat aacaatagtt caaaaattga
      241 tatacttgcc tttcattgat atcaataaat tcttcaaaca aagtacatac tcctttttat
      301 ctattattta gacttattcc gacagctaca tgaataacgt acaaatagat tcgttactga
      361 tttttcagag atactaccat gcgcagtgtg cattgtcaat tattcttgct cttattttcc
      421 atttgtgttc ctgttatttc ctgtttctca aatatgttag taaacaaaaa tagatctggc
      481 aaagctacaa cttcgccagg caatacagca ctctcgaatg aaatattcac tcaaaaatct
      541 atcgttttat tcaattttag atctgttgct tttattcttt atttcactcg atggaagaga
      601 gaacgaaaat agcttgctcc tacaatcaaa atgagtaaca cacccatcca tgtaattagc
      661 ttgttcaagg ttctactacc agtttctggt aaatcgcttt ttctattatc tgtttctttc
      721 gctttttctt gatgatcaat tccacgagaa acaaaatcag gctgttttag cacacttttt
      781 aggttgctcc ctgcatttct cttttgttcc ttagttggta tgataggtac caaatcaagt
      841 tcattattta cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct actctcacta
      901 ctcatgcttc ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg
      961 cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct cgtgttttct
     1021 gtgctaattt cactgctcgt actctctgta ctgctttcac cgctcgtgtt ttctgtactg
     1081 ctttcactac ttgtgctatc cgtactgctt ggcggtccaa caactgtcca actgcttggt
     1141 ttcaaattct ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc aactttatct
     1201 ttactcttga tctcatcaat cgatagttct gcagaaatca ttattgtata ctcaatacca
     1261 gcggtccatt gtacgtcttt taaataaact tccccaataa ctttttctga acttccatta
     1321 ccatcccaac catatgttag aacagcatgt acatcactag cattcacgct tttgtttggt
     1381 ttgactggaa tagtaatgac ttgttgtgtc atgtcataaa caatccgatc agttttcaca
     1441 gtaaatgaaa gattctcatt tgcccgttca tttgcagaag tactgattcc aatcattgct
     1501 gcaattatac tcagcccaag aataacaaat aatacggtcg ctgttttctt catttctatc
     1561 actctccata taattacctt ctatttgata aaggaattat ataaaatgaa aatgatgaga
     1621 acacgacagt cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt gaatttgttt
     1681 actttttcgt ttaatcaatg acagacttaa aaaagcaaga ccgatatcag ccttgctttc
     1741 ttcatctttt attgataaac agcagtgtat ttcttcatcc gctttgtttt atatgcatag
     1801 agtaacaaac caacaatcaa gactaacaag aataatgtag ttgatataga tgcttcagcc
     1861 ccaaagtttc ccccagtaat tattgtattt tgcgattgga tcagagtgga gaaaaaacct
     1921 tgttcttgcg ttccgctgac tggtataccc aaaatagctc cttgtgtcca attccaagta
     1981 atatgagcac caatagccgt aaataaactt cctgtactgt agataagtgc gctgaaaaag
     2041 caacctgcta aaaagatatt gaaaactgcc agaaatgtca tgttaggatt caatccgtgc
     2101 aaaagagcaa ataatgcagc agatccaatc attgctatta acggaccaaa tctaagcatc
     2161 tgatgctgaa aaaagccacg gaataaaagc tcttctgatg cccccactgc aataaataaa
     2221 ataaaactcg ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg atacgccccc
     2281 gtcaaatata atggactagt aataagcaga aacactccaa ttccaacaac gagacctccc
     2341 gctaaatacc cccagtcttt tcctcgaaca gaaacaccat aatcttctat tctttttcct
     2401 tcgaccttac tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc gaccgtaccg
     2461 gcaattgctc caatccattc actatttcca ataaacatat ccgcaagtgc attattgaca
     2521 agaattccta aaacaaaacc aacaaagact attaaaacag atccaatcaa ccgaataatc
     2581 ctgatcatct ttcatccctc ctttatatta agtttaacaa cccttttctt tcttgtctcg
     2641 tgaaaactat gtaaatgttc tccatcatag agagaataga tttttacgaa ctaagaatat
     2701 ctacagcaac cttacgaaaa tgaaagactg tctccctaat tcttctgtta gactgttttt
     2761 aataaggaaa ggatgggaaa ttttttgaaa aaacaactac taattatgtg cctttcagca
     2821 tgtgcaattt tactattctc aggttgtagc aagtccaatg aaacgattgc aacgtttaaa
     2881 ggtggaacac ttacagttca ggatttttat gataacccaa aagttaaatc aaataatcaa
     2941 acactattaa aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg aaaagagatt
     3001 tcttcaaagg aagtaacaaa agaatataat gaacaaatca aaaaactcgg tccaaactac
     3061 aaggaacaat taaaagcagt tggccaaaca gaagagacat ataagaaact ctttaaacag
     3121 atgctggctt tccaatacgg tttgaaagcc aatgtaaaat taacagataa agatctagac
     3181 actgcttgga aagaattcta tccagaagtt agtacacaga tcattctatt ttcgactaaa
     3241 gaagaagcag ataaagcaaa aaaagaagca aatgaaggag aaaatttctc caagcttgtt
     3301 caagcttatg gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa ttttgattca
     3361 accaatccag agattcctac tgaagtaaaa aaagcagcat ttaaactaaa aaacggggaa
     3421 gtttctgata tcattcctgt aacagatcca acgacttatc agcaaagcta ttatctagta
     3481 aaaatggtaa aaaaacagga taaaggttct aataaagaca aatacaaatc tgagctagaa
     3541 aaaattgcta ccgaagcaaa aactcttgat acggaattca tggataaaac aatccgtaaa
     3601 gtgatgaaaa aagacaatgt aactattaaa gatccttatg taaaaaatat attcaaataa
     3661 acaaatttga cccaattggg gatgtggcaa aatttgagaa gtgatttttg gaaattcctt
     3721 cttatttttg aggcaaacca cttttgccac aacttcatcg gggccttttt ttatttgtac
     3781 caaaacaaat cactggattt gttacaatca tgaggatata atttaaaaaa gttttttatt
     3841 tctatcacat gaatcaatgc gccattaagt agattctttc atgcttccct attttttcac
     3901 ttgccgtatc gcttcaaaaa caactatcat aaactcaaac tcgactgaaa agttatttta
     3961 aaaaagacta aaaatatccc ttctggactt gttcttctta ttctgttata ctagagaaac
     4021 acgaaagata tttttatgcg acacttttca tttgttttta tgaaatttat tcaaaactgg
     4081 cgtaacttca gagttacaag gatgtattag aggttaggga atacatcgtt taaatgagaa
     4141 ataagtaata gttagtaata gtcacccaaa ctttaaatta atgaatattg aatcgtcaga
     4201 tagcctcctc gagttttcga gaaggctatc gtttatttta tggagaaatt gattcagaag
     4261 taatcagcga ccttttttag ttcaccaatc gtttctgctt ggaaagtttg cacccaatct
     4321 tcttggaagc ccctcactgc tttatcaatc gagggcatct ctaatacttg atctagtagc
     4381 tctttaccta acgttatcgc tgagctccag atcgaatggc tgtagtgact tgttcacaat
     4441 ttttgaaact agctgctaat atttttgttg gtctttgcgt acgttcgatc tcagtccgca
     4501 acgaatcaat cactttcata gcatcaatgc cttgattttg catcctgtta taataaggtg
     4561 caatataatc tgctccatta ttaatagcta aatacccttg taattctgtg taaatagctg
     4621 taccggtaat caaataacct tcttttttca actttttgat cgcttgaagg cctgcttgat
     4681 tcaccggcac cttgataaac gtatcttttc ccagttcttc tatgataact cgagcatcct
     4741 ctatcatttc ttcttccgtt cttcctattg tctgaatatg gataggaatc tgtccgatta
     4801 tttttttgat ttctttcata tgcgagaaga agtttgacgc tccttctttt ttcagaattg
     4861 taggatttgt tgttacgcct gttaaattca aaaactgttg acaagaataa atactttttg
     4921 tatttgctgt atcaagcatg atttccatat agctttcccc cattctgatc ctcttgatcg
     4981 attttaatga ttgtcccctt tcttaaatgc ggcatttcct tatcttctac atacattgtt
     5041 cctggaagtt ccggctcatg agatccatca aattttatcg tgatatgtcc taattcattc
     5101 aaattagttt ggacaacatc tcctactgct gtgattacat acatttgttt atcaaaacag
     5161 agcttcatat cacaagttat ttcttttctt acctgattca attcaatctt ataacaataa
     5221 tctgttaatg tttccggtgc ttcatctcca aataaaatca ttattttttc tttcctgaat
     5281 acttctgctt ctataccaac atctgttaca accgtttcaa agatcgtcat ctctgttcct
     5341 cctcgtaaaa tatcatttgt ctaat
//
LOCUS       DADVXO010000141         5300 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.146, whole genome shotgun sequence.
ACCESSION   DADVXO010000141 DADVXO010000000
VERSION     DADVXO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.146"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..754)
                     /locus_tag="KB950_002493"
     CDS             complement(<1..754)
                     /locus_tag="KB950_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858662.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSE"
     gene            complement(966..1862)
                     /locus_tag="KB950_002494"
     CDS             complement(966..1862)
                     /locus_tag="KB950_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6858663.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(1855..2535)
                     /locus_tag="KB950_002495"
     CDS             complement(1855..2535)
                     /locus_tag="KB950_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858664.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2536..3318)
                     /locus_tag="KB950_002496"
     CDS             complement(2536..3318)
                     /locus_tag="KB950_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858665.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3311..4228)
                     /locus_tag="KB950_002497"
     CDS             complement(3311..4228)
                     /locus_tag="KB950_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6858666.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4392..4715)
                     /locus_tag="KB950_002498"
     CDS             complement(4392..4715)
                     /locus_tag="KB950_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858667.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1559 a         1112 c          888 g         1741 t
ORIGIN      
        1 cttcagaatt attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag
       61 caaggtggat agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca
      121 tgtatcgatt aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg
      181 cttcaccaat gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa
      241 agccacccca ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag
      301 ctttgatgcg cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt
      361 ttctaacatc tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa
      421 gattttgaat ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac
      481 ggtcctccgg agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt
      541 ctttttttcc aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca
      601 ttttttggat tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat
      661 ataattgttc tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta
      721 ttttcatttg ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta
      781 agtgtacaat tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt
      841 tctaatattc ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct
      901 tttttctcta ttaaatatat ataagataat acatttttct actagttctc actaatcttg
      961 tataattaaa ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg
     1021 gtcatctctc ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta
     1081 gattccgtat ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca
     1141 gaggtttgcg ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct
     1201 tttgaaaatg tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt
     1261 tgcagcactc gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca
     1321 gcgaacgcta cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct
     1381 tccacctctt tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt
     1441 cgagcaagat cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg
     1501 atcgttaaat acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa
     1561 ccgttaatag cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg
     1621 atgttttctt ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta
     1681 ttcagtgcat ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca
     1741 ccatattctt gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt
     1801 tgcaggaaga aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc
     1861 aaatctaaac cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt
     1921 ccctcgaaga ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg
     1981 ccaaactgtt tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag
     2041 cttcagcaga tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc
     2101 cctcgactct ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac
     2161 ttggacagaa gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc
     2221 aaatggtttc gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg
     2281 atcgcttttt gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa
     2341 caatgcttca cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata
     2401 tgtgcttaac aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg
     2461 ttcaagaacc tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt
     2521 ttccagcagg accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga
     2581 tgataccacg attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc
     2641 ttgttgcgca aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc
     2701 caagcctttg atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa
     2761 agaaagaagc aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga
     2821 taatgtccct aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata
     2881 gtttttgatt ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag
     2941 tcccgtgatc aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac
     3001 ataataagtc attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa
     3061 ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat
     3121 tttttctacc attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa
     3181 ttcattgccg tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac
     3241 gaccatcagt cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc
     3301 agttaataga ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt
     3361 tttgcccaac taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga
     3421 tggctgctac ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat
     3481 aatccctata accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa
     3541 tttttataaa ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt
     3601 gtccttcatg caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc
     3661 tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt
     3721 ctgccatccc tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat
     3781 tcgtgatcag tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg
     3841 ctttcttttt ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat
     3901 ccgacacacc gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat
     3961 aaagagcagg ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg
     4021 ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa
     4081 aggtagactt acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt
     4141 cgattgatac tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt
     4201 tcaagaccgt ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt
     4261 atagttgacg ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa
     4321 ttgaatgtca aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt
     4381 atacgcttgt atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc
     4441 tcttcactga aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt
     4501 ataaggataa ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt
     4561 tgcgttgtct tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
     4621 atggtaatcg ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc
     4681 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
     4741 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
     4801 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
     4861 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
     4921 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
     4981 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
     5041 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
     5101 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
     5161 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
     5221 ggttttttct tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg
     5281 taaatatatt ttattatatt
//
LOCUS       DADVXO010000142         5258 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.147, whole genome shotgun sequence.
ACCESSION   DADVXO010000142 DADVXO010000000
VERSION     DADVXO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.147"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            32..388
                     /locus_tag="KB950_002499"
     CDS             32..388
                     /locus_tag="KB950_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBB6858668.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="KB950_002500"
     CDS             404..754
                     /locus_tag="KB950_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBB6858669.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="KB950_002501"
     CDS             783..1562
                     /locus_tag="KB950_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBB6858670.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="KB950_002502"
     CDS             1573..2235
                     /locus_tag="KB950_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBB6858671.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="KB950_002503"
     CDS             2254..3360
                     /locus_tag="KB950_002503"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBB6858672.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="KB950_002504"
     CDS             3350..4441
                     /locus_tag="KB950_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBB6858673.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="KB950_002505"
     CDS             4454..5194
                     /locus_tag="KB950_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBB6858674.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1687 a          864 c         1253 g         1454 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaa
//
LOCUS       DADVXO010000143         5229 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.148, whole genome shotgun sequence.
ACCESSION   DADVXO010000143 DADVXO010000000
VERSION     DADVXO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.148"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(129..311)
                     /locus_tag="KB950_002506"
                     /pseudo
     CDS             complement(129..311)
                     /locus_tag="KB950_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            298..495
                     /locus_tag="KB950_002507"
     CDS             298..495
                     /locus_tag="KB950_002507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858675.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(538..1113)
                     /locus_tag="KB950_002508"
     CDS             complement(538..1113)
                     /locus_tag="KB950_002508"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBB6858676.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1268..1794)
                     /locus_tag="KB950_002509"
                     /pseudo
     CDS             complement(1268..1794)
                     /locus_tag="KB950_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2251..3762
                     /gene="gadC"
                     /locus_tag="KB950_002510"
     CDS             2251..3762
                     /gene="gadC"
                     /locus_tag="KB950_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBB6858677.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3775..5175
                     /locus_tag="KB950_002511"
     CDS             3775..5175
                     /locus_tag="KB950_002511"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBB6858678.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
BASE COUNT         1597 a          905 c          962 g         1765 t
ORIGIN      
        1 ttcaataaaa atatattaca ataataatcg taatagctaa tgaaacaatt tctcaatctt
       61 tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa
      121 ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta
      181 tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct
      241 agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttattg
      301 aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta caacaaaact
      361 actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca tgatgtttta
      421 cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc tgatcctcaa
      481 cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt ttttatttca
      541 atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac ctcctactac
      601 aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca ttacagcctt
      661 tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa atgtcaataa
      721 tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat agcaaattac
      781 tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc tacttaaaaa
      841 ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa tctccactaa
      901 ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa cacctatacg
      961 actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta tgataactct
     1021 cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct ttccactatt
     1081 cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct tttacaattc
     1141 aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt gatatgtcta
     1201 taacatctac tatccctaaa accaatttat aagccattag agatggtatt cctgaacatc
     1261 tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc
     1321 gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag tcagcacttt
     1381 agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct caatcaagtc
     1441 tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaatgt
     1501 gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat tgcggtcgcg
     1561 cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt
     1621 acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt
     1681 taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga ataatccatt
     1741 caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt tcatatggaa
     1801 gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt
     1861 ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat acttgctcaa
     1921 gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca gctttttttg
     1981 gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt ttcactaaag
     2041 atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt tctaaaaatg
     2101 aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt cttgtagaaa
     2161 agaggtagac aaaataatgc aagctcaaat aagcgatttt ccataacctt ggtaggaatg
     2221 acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc tctattcggt
     2281 ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac ctttgcaaca
     2341 tcacaattac atttggtttt ctttcttctt ttgggaggta ttttatggtt tctaccagtt
     2401 gctttatgtg ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg gattttcagt
     2461 tgggttagtc aaactttagg agaacgtttt ggttttgcag ctattttttt ccaatggttt
     2521 caaattacag taggttttat tacaatgatc tacttcatac taggagcgct gtcgtatgta
     2581 attaattttc cagagcttaa tacagatcca tttatcaagt ttattggtgt attagtcatt
     2641 ttctgggtac tgactttttc acagttgggc gggaccaatc gtaccgctaa aattgctaaa
     2701 gcaggatttg tcattggaat tgttattcct tcgatcattc tctttggttt agcagcagcc
     2761 tactttatcg gcggtaatcc gattcaaatg gctattaatg cgaaagcttt tattcctgac
     2821 ttttctcagc tttcaacttt agtagtattt gtatctttta tattggctta tatgggcgta
     2881 gaagcttcag catcacatat taatgagctg gaaaatcccc aaagaaacta cccattggca
     2941 atgtttttac tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt ctcagtagca
     3001 gcggttattc cacaacatga cttgtctctc agtgctggtg taatccaaac atttgaaacg
     3061 cttattttac attttaacag tcatttacat tggttggtaa aagtgattgc tctcatgatt
     3121 gcctgtggcg ttatgggtga agttagttct tgggtagttg gtccatcacg agggatgttt
     3181 gcagctgctc agcgaggtct gttaccagtt ttcttccgga aaactaataa acaagacgtt
     3241 ccagttccgt tgattatgat acaaggaatt atcgttacta tttgggcagc agtcttaact
     3301 tttggtggag gaggcaacaa tttatcattc ttagtagcaa tctccttaac tgttgtcatt
     3361 tatctcgtag gatatttact atttttccta ggttactttg tattagtttt taagaaaaaa
     3421 gacttaaaac gaacttacaa tgtaccagga aaaactgttg gcaaaacgat tatcggtgga
     3481 attggtttta ttttatcagt gttcgcattg cttatctctt ttgtaccacc agcttcaatt
     3541 gcagctagtg agcaccgaac ataccaaatc atactactta ttagctttgt agtgactgtt
     3601 cttttacctt ttatcattta tgaagtacat gataaaagtg ctcacacaac tattgaggaa
     3661 cctactcacg tgaaagctga agacgttaat cccgctgttt atccagctgc tagaggagag
     3721 caccacgtta caaaaaatga tgaacatatt ttaaaacatt aggaggaatt cattatgtta
     3781 tacggaaaag ataatcaaga agaaaaaaac tatttggaac caatttttgg ctctgcaagt
     3841 gaggatgttg acttgcctaa atataagtta aacaaagaat ccattgaacc acgaattgct
     3901 tatcaattag tacaagacga gatgttggat gaaggaaatg cgcgattaaa cttagctact
     3961 ttttgtcaaa cgtatatgga acctgaagca gtgaaattga tgacccaaac gttagaaaaa
     4021 aatgcaattg ataaatcaga atacccacga acaacggaaa ttgaaaaccg ctgtgtaaat
     4081 atgattgctg atttatggca tgctccaaat aatgaaaaat tcatgggaac ttcaacgatc
     4141 ggctcttcag aagcctgcat gctgggtggt atggccatga aatttgcttg gcgtaaacgt
     4201 gctgaaaaat taggtcttga tattcaagca aaaaaaccta acctggtgat ctcttctggt
     4261 taccaagttt gttgggaaaa attctgtgta tattgggatg tggaactgag agaagtccca
     4321 atggatgaaa aacatatgtc aattaatcta gatactgtca tggattatgt ggatgagtac
     4381 acaattggta ttgtaggtat tatgggtatt acttacactg gtcgttatga tgatatcaag
     4441 ggtctgaatg atttagttga agctcacaat aaacaaactg actataaagt atacattcat
     4501 gttgacgctg catcgggtgg cttttatgca ccatttactg aacctgatct agtttgggat
     4561 tttcaattga aaaatgttat ctcaattaat tcttcaggtc acaaatatgg tttggtatat
     4621 ccaggtgtgg gttgggtctt atggcgtgac caacaatact taccagaaga attagtattt
     4681 aaagttagtt acttaggtgg agaaatgcca actatggcta tcaacttctc tcatagtgca
     4741 gcacaactaa ttgggcaata ctacaacttt gtacgctatg gctttgatgg ttatcgtgat
     4801 attcaccaaa gaactcatga tgttgctgtt tatttagcca aagagatcga aaaaactggt
     4861 atttttgaaa tcattaatga tggatcagaa ttgccagttg tgtgctataa gctgaaagaa
     4921 gatcccaatc gcgaatggac actatatgat ttatctgatc gtctgttaat gaagggatgg
     4981 caagtcccag cctacccatt acctaaagac ttggatcaat taattattca acgcttagtt
     5041 gttcgagcag actttggaat gaacatggct ggtgattatg tacaagatat gaaccaagca
     5101 attgaagagt tgaataaagc tcatattgtt tatcataaaa aacaggatgt gaaaacgtac
     5161 ggctttactc actaaaattc ttcagagaaa tataaaagaa gttaattaac taataaaagg
     5221 agttgtgat
//
LOCUS       DADVXO010000144         5199 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.149, whole genome shotgun sequence.
ACCESSION   DADVXO010000144 DADVXO010000000
VERSION     DADVXO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.149"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            147..1664
                     /locus_tag="KB950_002512"
     CDS             147..1664
                     /locus_tag="KB950_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBB6858679.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1636..2925
                     /locus_tag="KB950_002513"
     CDS             1636..2925
                     /locus_tag="KB950_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6858680.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3100..3996
                     /locus_tag="KB950_002514"
     CDS             3100..3996
                     /locus_tag="KB950_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6858681.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            4010..4873
                     /locus_tag="KB950_002515"
     CDS             4010..4873
                     /locus_tag="KB950_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858682.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4879..>5199
                     /locus_tag="KB950_002516"
     CDS             4879..>5199
                     /locus_tag="KB950_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBB6858683.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLI"
BASE COUNT         1639 a          802 c         1073 g         1685 t
ORIGIN      
        1 aaagttatat taaattattt gttttaatca ttattaatat tttggttaat taaaatcgat
       61 aaaaaataag taagtttaac atttatatta ttgacaacgc tttcaacgag gtgtattatt
      121 catgtataaa ttaaaggagg tcagttatgg aagccacaat gttgattagt agtgtcaagc
      181 gtgtaggcaa agttgatccg cggttgtaca gttcatttat tgaacatatg ggacgagcag
      241 tatatgaagg gatttatcag ccagatcatc catcagctaa taaaagtgga tttcgccaag
      301 acgtcattca cttggtaaaa gaattgaaca ttcccatgat tagatatcct ggtggaaatt
      361 ttgtgtcagg atttcgttgg gaagacagta ttgggcccat agcaaataga cctagaaggt
      421 tggatttagc ttggcggaca attgaaacga atgaagttgg gattcatgaa ttttataact
      481 ggtgtcagca tgtgggggcc gaaatcaata tggctgtcaa tctgggtact cgtggagtag
      541 atgcagctcg taatttagtc gaatattgta attttcctaa aggaacttat tggagtgatt
      601 taaggcgttc gaatggtcaa gagagccctt ttggaattaa gacatggtgt ttagggaatg
      661 aaatggacgg tccttggcag attggacata aaacagcgga agaatatgga cggttagcag
      721 aggaaacggc taaggcgatg aaactcgtag atgattcaat tgaggtggtt ttatgcggaa
      781 gttcgaatcg gcaaatgcct acatttggtg attgggagtt aacagttctt gatcaagcgt
      841 acgatcaaat agattatttg tctctgcatc aatattatgg caaccaaaaa aatgatcttt
      901 ctcattatct tgcacgttcc ttagacatgg atcggttcat ttctgaagtg acagcgatgt
      961 gcgatacagt caaggcgaag aaacatagta agaagcagat caacctgtcc tttgatgaat
     1021 ggaatatttg gtatcattca gcggaacaag atgagcaagc agcgccttgg cagattgctc
     1081 ctcctttact agaagataac tataattttg aagatgcatt actattaggc tgtctattaa
     1141 tcactttatt gaaacatgca gatagagtaa agattgcttg tcttgctcaa ttggtaaatg
     1201 ttattgcacc aatcaggaca gaaacaaacg gagaagcttg gagacagacg atctattatc
     1261 catttatgca agctgctgtg tatggacaag gagaggtgtt aagcccacaa attcgttcag
     1321 caaactatgc gacggaagat tttgaagaag tgccgtattt agaaagtata gccgtaataa
     1381 atgacaaaga acttgtcata tttgcagtta accgtggaga agaagaaatg aatctaactg
     1441 ttcacttgtc tgaattatca ttagagggag tgattgattt ttctgaaatg agtggatttg
     1501 atacaaaagc aactaatctt cctaaaagtg aacaagtaaa accaagtcat tcaacaaatg
     1561 taaaagctga agatgggaaa atcaattgtt tattgaaacc gctatcttgg aatgtggtca
     1621 gatgtcgttt atcagatgaa ggaggaacaa aaaatgaaag gtaaaaaatg gttaattggc
     1681 tctttgttgg tagctgcaac aggtatttta ggagcttgcg gtggcggaag ttcagcagaa
     1741 tctgattctg ataaagaagt gactttgact ttttggaatg ggtttactgc atctgatggt
     1801 gaaatattgc aagaaatcgt taatgaattt aataaaacaa atgataaaaa catcaaaatt
     1861 gaaatggata tcatgacttg ggccaatttg aatgaaaaac ttcctacagc gatttcttct
     1921 aaaaaagcac ctgactttat tgcattaaat tatccagatt ttgcacagta tgtagaaaac
     1981 ggagcggtac aaccattaga cgatttctgg gaatatgatg gagtagataa atctgatttt
     2041 aaagaaacgg ctattgaatt aggtgaagta gaaggtaaac agtattttgt ccccatgcag
     2101 gtacaaggca tgtatatgta ttggaataat gatttgttta aacaagctgg attagatact
     2161 gaacaaccac ctactacttg ggaagaattg acagaaatgg caccaaaatt aactgatagt
     2221 agtaaaaatg tatcaggttt tgtcttcaat aaggatggaa ctgcaccgtt atataactgg
     2281 atgttagcaa atggcggtaa attagtgaac gatgattata ctaagtctga atttgcgtct
     2341 cctgagaacc ttaaaacgtt aaaagcgatc caaaaaatga tccatgtaga caagactggt
     2401 ccagaatcga tttctggcgc cgaaatggac aatttaatga atgctggtca actagccatt
     2461 gaaatcaatg ggccatggtt aaataacgga ttgaaagcaa acgagattga ttataatgtg
     2521 actaccttac cacaagggag tgattcagac aaaaaaacag ctattttaga tggcgtagga
     2581 tatgccattc cggcaagtac agataataat aaaaaagaag ctgtctatga gtttttgaaa
     2641 tattggaata caactgaaat tggtaaaaaa tggagtatcg aaaatggttt tcctgcctac
     2701 ttggattcag tagcaaaaga tgaagaagta aaaaatgatc ctattgtatc tgaattatcg
     2761 aaacaaatgg actatgcaga gccatattta ccaggtttta cccaaatctc tgcgatcaat
     2821 aacgatatca tcaatccact gattgaaaaa ttattagcag gagaagatcc tgagacactc
     2881 atgaacgatg cagatgatgc catcaatcaa ttactgcaaa aatagtacag aactttatac
     2941 aggatagact gttggcaaga tacagatatc tgaatgatcg acctagtatt tgcagatagg
     3001 tgttattgca aaaatcacag cgattgttcg gatatcttat tcagattcgt ttaaaccaag
     3061 attgtatatt ttatcaaaat gaaagatgag gtggactata tgaacggatt caccaaatat
     3121 tggaatcgtc ctgatcgtgc tgcgtatttg tttttaactc catctttttt agttttatta
     3181 ttatttactg tcataccgct tttaggaaca tttgctatta gttttacaga tttgaatatt
     3241 ttctttacat caagaaattt tgtaagtttt gaaaatttca cacgtatatt tagtgatgaa
     3301 cgagcagtta attcgttgat tcattcttta tatttcaccg tactcgaaac gccaacacaa
     3361 attatcgttg gtttattagc agcagtagca ttatctaaaa ataccagatt caatcgtttt
     3421 tgtcgttcga cattttacat tccagttatt tgttcattga cgtcaattgc gattattttt
     3481 tctatgttgc ttgatccgaa tgtaggggcg attccttact tgttttcaca agtcggcttg
     3541 ccgatccctc aattttttcg cgatccaacc cttgctatgc cgacggtagc catcatgact
     3601 gtttggaaga attttggtgt tactatgacg attctcttga ctgctattca gggaatctct
     3661 ccctctttgt atgaatctgc tcaaatggat ggtgcaacaa atcgacagca gttttttaac
     3721 attacgctac ctcagatcgt tccctcctta ggtttttgta ttttaacgaa tctgattggt
     3781 tcgatgcaag tgtttgacca agtatatgtt gcaaccgctg gtggcccaca atttaagaca
     3841 gaaacagctg ttcaatatat ttacagtcga ggatttacag caccttatga attgggctat
     3901 gcatcagcac tgtcttcggt tttgtttatc attgttgcaa ctatagctat ctccactaat
     3961 gtgtacatgt cacggaaaga aaaacaaatg aaataggagg gaaacctgta tggcaccaga
     4021 aagctcgatt acagttactg tgaatagtcg aaaagcagtt agtggtaaaa aaatcattcg
     4081 aacgattagt atgctagtta tattgttggt tattgcgttt ccttttttgt ggctgattat
     4141 ttcttctttt aaacacgaaa aagacattat ctcatttccg cctagaatat ttgcagatag
     4201 ctatacccta gataactata ttaaggtttg gacaacgatc cctttattag attacatcaa
     4261 aaatacagtg atttttgctg gagggacagt gatcacttct gtattctttg attctcttgc
     4321 ggggtatgca tttgctcgta tgcgtttcaa aggaaaatca gttttgttct atttcgtttt
     4381 gctgacaatg atgatcccat tccaagtctt catgattcct ctatttattg aagtcaattt
     4441 gcttggcatg cttgatactt atgcaggttt gatcattcct cgtatgacaa ctgcatttgg
     4501 tatcttcatg atgcgttcct tttttattac tttaccagat tctttggaag aggcagcacg
     4561 cattgatgga ttaagtgagt ttaatatttt tttgaaaatc atgttgccat tgagcaaacc
     4621 tactctttta tcattaggga ttttcacgct gatgaatagt tggaatgact tgctttatcc
     4681 attgatattg acaagtagtt caaaaatgag aactttacca gcagggttag ctttatttac
     4741 agggcagaac atttcttttt atggacctgt aatggctgga acggttattt caatgctgcc
     4801 attactagtg gtttatattt ttgcacagaa atattttgtt caaggaacag ctatgtctgg
     4861 aatgaaagag tgaggaagat gaaaatagtt gagactaaac tttatggaca gttgattgtg
     4921 gtttctgatt acatcttatt aggtattctc tgggtaatcg tgtgtttgcc tgttattact
     4981 atcgtgcctg ctacatgtgc tgttttttat gtaatggcgc aatggaaagg tgagggatca
     5041 ggaaatatca tttatttttt ctttcaagga ataaagcaac attttggtaa gaacttgttc
     5101 cttagtctgt taacgatcat tatttatttt cttgttaatc aattattaga agaacagaca
     5161 atcgtacttt caattagtgg atatgtagtt ttacttatt
//
LOCUS       DADVXO010000145         5181 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.150, whole genome shotgun sequence.
ACCESSION   DADVXO010000145 DADVXO010000000
VERSION     DADVXO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.150"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(412..1281)
                     /locus_tag="KB950_002517"
     CDS             complement(412..1281)
                     /locus_tag="KB950_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6858684.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1301..2422)
                     /locus_tag="KB950_002518"
     CDS             complement(1301..2422)
                     /locus_tag="KB950_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBB6858685.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2424..2819)
                     /locus_tag="KB950_002519"
     CDS             complement(2424..2819)
                     /locus_tag="KB950_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6858686.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2838..3644)
                     /locus_tag="KB950_002520"
     CDS             complement(2838..3644)
                     /locus_tag="KB950_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6858687.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3637..4425)
                     /locus_tag="KB950_002521"
     CDS             complement(3637..4425)
                     /locus_tag="KB950_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858688.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4439..4915)
                     /locus_tag="KB950_002522"
     CDS             complement(4439..4915)
                     /locus_tag="KB950_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858689.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4987..>5181)
                     /locus_tag="KB950_002523"
     CDS             complement(4987..>5181)
                     /locus_tag="KB950_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6858690.1"
                     /translation="ISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHY
                     YSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1626 a         1124 c          759 g         1672 t
ORIGIN      
        1 ttgctttatc tagtataact taaattttaa ataaatcgtt atttattttt actctcactt
       61 aaaaaagcac taaaattata agagagggag gtaatggtct gaacaccaag aattgttcca
      121 acatcgaatt gcttaactaa tgagttcaaa gcttaattat gtggctctta tatttaccga
      181 gcgtggattg cgatctcttt agcgaaacaa acatctttca taaaaaaaac agccatatcc
      241 gctttcttgg atatgactat ttactactac tttactatct ttcattcttc ttgtctcact
      301 tatgataagg cgctgggtat catgagtgaa tgatattgct gaatacaaat agtccgacca
      361 cattaagttg atcggacttc ataagcattt tattgactaa tacttttttg cttaagaatt
      421 gattctaaca tcttttgcta aagttaagca tcctatagtt cctgcattat ctgctaactt
      481 aggtgtgaca atgtattctt cgatatttgg cgtatcgaca tagccgttaa ttagttctaa
      541 aaaacgtctt tgtactttct ttttcagatg ttcttgttgc atgacaccac caccaaaaat
      601 tataatatct ggtgaaagca tcagggtcgt attacaggca cattgtgcaa tataatccgc
      661 ctcgatttcc cagaacggat cttctataga aagatcttgt cccttggttc ccgtacgcat
      721 ctcgatagcg ggtccagatg ccaacccttc gagacaatca tgatgaaaag gacaagtacc
      781 agagaaagta tctcctgaat gtctcttaac taacgtatgc cccatttctg gatggctaaa
      841 cccttcaaca aaaatcccat cttgtatagc tcctccacca attccagttc caacagtgta
      901 ataaacaaca ctggataaac cttttccatg acctgaaaca tactccccgt aacaagcagc
      961 attcacatcg gttgtccagg caattggaat ttcgaaatgc ttattcaacg ttccaacaaa
     1021 atcaaaattt ttccaggcag tttttggagt agaggtgata tgtccatagg tctttgattc
     1081 acgatggata tcaatcggtc caaatgaccc tactccaatg ctttctaatt gttctttata
     1141 gtgattaaag aaatcaatta ccaaactcat tgtttcattc ggtgtagttg taggaaaact
     1201 cactctttct aaaaccttta aatcatcatt gccaatggca caaacaaatt tagtcccacc
     1261 tgcttctatt gagccataca tttcttactc caccgtcctt ttatctagga attcgttctt
     1321 ccctgtctaa tagtttttcg aaaaactcat gtggttcctt ttggtaccaa taggcaacag
     1381 aagaaatatc gtcttgacgc tcataaagct tgatgtcatc atttcctagg gtttggaaag
     1441 taacacgtaa attttctttg aaactaatgg ggtcgggtaa atgccagcgg tacaacccat
     1501 gcatcggtaa agcatcatcg ccaaaaccat gaactggatt tggattaggt tttccagcac
     1561 taaaataatc ccgggtgtga tctcttttag attgaaaagg atagcccata aatagcgtat
     1621 taaaattttg agcatatgcc ctttctccaa agttcttttt atggaaagcc caagcgccgc
     1681 caaaataatc ttctgctcct gtagatgtta ttgtagggaa atcttcatct ccatctatat
     1741 aaaacttaaa ttctccttcc ccccaccagt atctttccaa tgcgcacaaa gcaaagtagg
     1801 ttcctacata atacccagtg cctttgattc catcaataag ggtgtaatct tttccttttg
     1861 tcgtgatggg ttcacggttc catttagcat gaaaatacat cgcatcatcc ggtacttcag
     1921 tcaatgcata attaaatgta taaaaaatgt gcttaataaa ctcaggatgt tcattcgtta
     1981 aagtaatctt agcttttttt cgaaatggca tctcaaaata gcaattcatc cctgatgttg
     2041 gattcactac gattggtaag gaattcactt cgcatcttgt cccaaaccca ttacaaaaaa
     2101 aatctcctaa tggactttct acagaaggag tggattcatc atcccaataa atacgcaaaa
     2161 ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc atccaaaaat
     2221 gacgtaaaat tccagggcct tccatttctg ctaaagtaat tgtctctcca gcatttatcg
     2281 gaacagaagg tgaccctttt ctcccaattc ctagatgact cgctgtcatt gccgctttcc
     2341 ctttttcacc atttttattt tctggtgaaa tagttctact ttcctctccc ttgtggagcc
     2401 atatattatt taaacaattc attttattcg tcctctatcc tttcttcact tttttgatta
     2461 ttcatataga tgatttgttg ttgtgcctcc gcaataattg ctccaatatc taaattatca
     2521 tttcctaact gcaaagcgat tgacattgcc aagggaagat tcattcctgc aattacgtgc
     2581 actctgtcag aaatataggt aaaaaacttt tgatttacac ttcctccagc catgtcagtt
     2641 aaaacaatta tttcgtctgt agtatccact aactctttaa tttgttcatc tatatcatca
     2701 atttcttcga catatgcaca taagctacta atttgattat gcatacctgt taaaaactct
     2761 agtgtctctt taaaaccctc agctagtatt gaatggctag caatgatcaa tcttctcata
     2821 attcaaatcc ttttccttta cgctaatatt ccaaatgcag ccataaccat cgcaaaaaca
     2881 ataacgagta gaatcaaaat ggtcatactg atttttttct tactgagtag ataatatgct
     2941 gcagcagtaa taattactgg aattaatgtt ggtaaaattt tgtccaacat tggttggatt
     3001 ttcatactaa cttcaccaaa agtgaattta agtggcgttg aaacactgat aactgaagca
     3061 attaatgatc caactactgt tagccccaat actgaagcag cttcagttaa ataattaatc
     3121 ttttcaccgt attcagtaac aatcttcatt ccctgcttat aaccaaattc aaagcctctc
     3181 attcggaaaa agaagattac catgattaaa atgaaccata aaaagattcc tacaggatta
     3241 ttttctaaag ccatataagc cgcaattgat cccatgatgg ttgggatcat cgtcatcaaa
     3301 agagagtccc caataccagc aattggaccc atcgtaccaa ctttaaagtc attaatcgca
     3361 tcttttgctt ctaatccttg cttttcctcc attgcaacac aagcacctaa aataatggct
     3421 gccagccaag gctgtgtatt gtaatactga aagtgattat ttaaagcttc gatgtattca
     3481 tcatcatctt tgtataattt tcttaaaata ggtgacaatg cgtaagtcac agatgcacct
     3541 tgctgtgttt gataattaaa ggtacaaact gacatcaacc aacgatagtt cgctttccta
     3601 agatcctttt tcgttacttg atatttttcg ctcattttat tcatcctccc caataaaccc
     3661 aacatcagaa gctcccgcag aagcagtgat ttgtgcatct ttttcagctc tatgatacac
     3721 attaaatgct aaagcgaatc ctatgatcgc cgctccaata ataggtacct tcaaaaaagc
     3781 ggctaagaaa aatccaacaa gtaaataact aatgtatcga cctgtaggca taaagcgtaa
     3841 caacatcgca ataccaacag caggcaacat ttttccggct aatgttagcc catccataaa
     3901 ccaattaggt aaaacatcca aaacgtaatt aacagcagac tgtcctaaag taacggcaat
     3961 aaatactgga attgctgcag ataaaccaaa gaaaattggc cccaaccaca aaacactatt
     4021 cattttctta aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa caaaaccatt
     4081 taagattttg acaattacat ctagttgcac ccctaacata cctacaggta gtcctattgc
     4141 taatccggct tctactccaa gctggctacc aaatgccgtt gcaataattg cgcacgttcc
     4201 atagtccggt acagatgagc caccaaaacc ggcaactcct aatgacatca attgaactgt
     4261 accgccaact atcaaacctg tttttatatc acccatcaaa attcctgtga ataatcccca
     4321 aaaaactgcg taatttacaa agatcatcgg aatcccataa taatcaacat gtttaataaa
     4381 tgctaatata gtcagcataa taatttgaaa aatagtaaaa tgcattatcc ttactccttt
     4441 atcctaattt tgattgaatt tcctgtgcct tatccgccgg aacaaattgt gtaacaattt
     4501 ctacaccaga atccaacaat tttttatacg tgctgatatt ttcatctgat gcataggctc
     4561 tagaagatag ctgtaatgca ttatctttaa caacagtacc gccaacaatt agtcggtcaa
     4621 ttctaatacc gttatctact aaatgaagga ttgtttccgg ctctttcacg atgacaaata
     4681 ctttttcctg atcgtatttc ccctttttaa aattcgcaat cgctgtttct tcagtgataa
     4741 ttgatacagc cattccagca ggtctagcta aacgcatact tgatttttgg atgtcgttat
     4801 ttgcgacaga atcatcgatc accatcactc tgtttgcttt agacattggt gcccattgcg
     4861 tgacaataat cccatgcaat aaacggttat caactctcga cataacaata ctcataaata
     4921 cttcctcctt tatctttcat tttcttacaa gcaattacca tgccaaaaca aaaagaactt
     4981 gataaatcaa gctcttttta cataatcata aacgtattta acctcatgaa tcgggatgtc
     5041 gacactataa taatgttcaa cgacactgaa agattgctta aaatgtgtct gaaatttttg
     5101 aaattcctca ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt gttttactaa
     5161 tcgttcaatt aagcaactaa t
//
LOCUS       DADVXO010000146         5178 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.151, whole genome shotgun sequence.
ACCESSION   DADVXO010000146 DADVXO010000000
VERSION     DADVXO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.151"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            247..1467
                     /locus_tag="KB950_002524"
     CDS             247..1467
                     /locus_tag="KB950_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBB6858691.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1464..4184
                     /locus_tag="KB950_002525"
     CDS             1464..4184
                     /locus_tag="KB950_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6858692.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4168..5112
                     /locus_tag="KB950_002526"
     CDS             4168..5112
                     /locus_tag="KB950_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6858693.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1924 a          846 c         1031 g         1377 t
ORIGIN      
        1 atacggaaaa atggagcata aattccagta caaatagtac ttcttcagtg tcaaacgaag
       61 cgaatataac atagatattt aggattgtga caaaaatcat gtcacaatct ttttttcgaa
      121 taaacttatt ttttgaagtc ggccatttat cgtaatttct ttgcataata cgaacaaatt
      181 ttcggttatt gatgaaaaaa aacattgaaa atggtatgat aaaagaaaaa aaggaggaaa
      241 tttttaatgg tgaaatttat ccatgcggca gatttacata tggatcggtc atttgaagga
      301 ctgacaactc ttgataaaac tgtacaggaa aaactattga agacgaatct gaccgtttta
      361 tcaaatatca ttgaacaggc aataataaat aacgttgatt ttgttttact tgcaggagat
      421 aattttcatc aaaatcgtcc ttcattaaaa atacagaaac atttttcaga acagatgaaa
      481 cggttagaaa agaaccatat acctgtattt ataatattcg gaaatcatga tttctatcaa
      541 aaagagcggt attggttctc ctttcctaaa aatgtccatt tatttacatc cgaaacggta
      601 gagacaaaaa aaataacgat aaaaagtgga gaaactgtct ctctcagtgg atttagttat
      661 cgtcaaccgt ggattcaaaa agacaaagtg atggaatttc cttctagaga actcacagat
      721 taccatattg ggttgtacca cggggaacct ggcgtctctc aaaaaggaaa ctatgctcct
      781 ttccaaccct caaagatgca agaaaaggga tacgattatt gggctttagg gcatatccat
      841 gtgccaacgg tcttaaatga aaagcaaaca attgtttatc caggtgcgcc tcaaggtcat
      901 actaaaaagg aacaagcttc tacatctatt ctactagttg aattaagtaa aggaagttgc
      961 caaatacatc cgattaaagt agcagaggtc tattggaaga caaaggaaat atcattgagg
     1021 aaagcaagaa cgacaaaaga ggtcattgtt cacattcgtc agcagctttc aaaagtagag
     1081 gatggattta gtttaattga gataaaatta aaagattatg accatctcaa tacagatgtt
     1141 ttggaacgga tacaatcagg agagctatta gactatttgt tagaggaatt ctcagatagg
     1201 ataaatgatt tctttatttg gcgtatttct ctcatagaga atacttttta tacgaagacc
     1261 ccacttgctg cttctgctaa attgatggaa cagttatttc aatactatca gacacctcaa
     1321 gatttccaac aaattttaaa tgaagtatat agtcatcaag aagcggctag aatattgagc
     1381 gaactgccgg aatatcaaga agaaacactt gaaaaagcca agcagctgct taatcaagat
     1441 tttttatttg aggaggatca ggaatgaaga ttttaaggat cgagattgct gcttttggaa
     1501 aatggcgtca aaaaagtttt gacttttatt caggcaatca gctgatctat gggggaaacg
     1561 aagcaggaaa atcaacgatt tatcaattta ttcaagcaat attatttggt ttcccttcaa
     1621 aaggccgtaa aaaaaaggat tatacaccaa aagacggctc agcctatggc ggcaaaatat
     1681 ggcttaaaca tcctgtatat ggtgaatttg ctgtcgagcg ctacaagcag caaaatcgcg
     1741 gaaaatcgaa ggtgtggctg ggggatcaag tgggaagtga tgagttacta gaaaaactga
     1801 tcgcaccttt gacgaaagag ttattccagc aggtatttac tttccagcaa gagcagttga
     1861 tggaactaga tcatttgaga gaaaaagagc tacatgatgc gctgatttct ttaggcatca
     1921 cgggcagttc tcaagtattc caaaagcggc aagaatatta tcagaaagca caacaattat
     1981 tcaagaaaaa aggccaaaaa ttactaatca atcaaaaatt aaatgaatgg aaagagctgc
     2041 aagaaaaaat tcatcaaaaa caagaacagg aagcttattt tggcaagttg atccaacaag
     2101 aacaggaata cgagtcgcaa cagcagcagt tattggcaca attaaaagag gcgaacaaac
     2161 aattttcatt agccagacag caagaactta atttttcttt atatgaagaa tggcaatctc
     2221 tcaaaaaaaa cagcactaca ggtcttcctg atgagcaact tcttttagat ctggagtctt
     2281 tctccaaacg gtatcagcaa acaaatgagc gtttggaaga attagaagca acactggaaa
     2341 agcatagcgg gatggatcag cagtcagcca gatattattt ttatttagag caggagcagg
     2401 aactaaaaga tctccagcaa cttcaattta cagcggaaca attaaacgaa gaatggcaaa
     2461 gattacaaat tgagactaga gaaacaaatt cagaattgaa gattttggaa caaaggtggc
     2521 attggcagaa aaatccgtta ccggaagaat ttttatatga agaagaatgg aagcatcttc
     2581 tggctgaaga aagaaggatt gctgatctag aaagccagat ttctgtgcgt ctccaaatag
     2641 ctaaagaaca gcaagagatg attgaagcag aagtgaatga cttggaagag acctatccag
     2701 cattattaca aaatcagtca gagatagcac cagtctcttc atctaaaccg tatatgctga
     2761 tggctggcgg agcaataatc attgcaggta tatttgtacc aatgcccttg aaagtgatcc
     2821 ttttactaat tggtgcaggc atgattggct atttcttcta caaaaggcaa ccaaaaatta
     2881 aaactaaaaa tgatttctct gagataaaag gaatgtggca agaaaaacta ggacaacttg
     2941 atctagcctt agctcaatta gctgaagcag aagaaaaaca aaagagaact ggagaagaaa
     3001 aacagattct tcagcaagaa gtatatcgtt ttgcggatca tcggcactta ggaaagatgg
     3061 atacgctcaa actcatgaaa gaatatggac aggaagtcat agattatcag aaattatatg
     3121 gcgtatttga gcggaaacat aaaagagaac aggaaataca agaaaaacta cgacaaatcg
     3181 accaaaaatt tgagtttgtt aaagaatggt taccgataca agataagaca atttcagaaa
     3241 aaatggtatt gctttcccgt tttatacaag agatggaaca gatgaaattt gccagaagct
     3301 atcagcaaaa tactttattg aagcaagaaa tcaatcaatt gaaaaagaaa caaaaagtat
     3361 ggatacaaga atacagcgaa caattgactc aagcaggctt atcttatcct tcggaagtac
     3421 ctgtcttttt acaaaaagcc aaacaagccg aacagcagat gaaaagatac aatgaattat
     3481 cggaaatgct tactccacta tttcctgaag agctgacaaa agagaaatta tctgtaagac
     3541 tgcgccaact gaaagaaaag caatcccaac tccagcaaga agaaaaagaa gcaagtgaaa
     3601 atcggcaacg actccaactt caagtagaag agttgcaaag agacggcaca ttggacgaac
     3661 tttatcaaca agctagccgg caaaaagcaa aattacagga attactggag gaatggggag
     3721 cacttgaaat cgccgggaca ttcttaggtg atctttcaac agagatgagt gaaagacaat
     3781 taccccagtt gttaagagca gcaggtgatt atttaggtat tttaacaaaa ggaaattatc
     3841 agaagatatt actgaaaaat gatacactga ctttaacaga cggatataca gaatttccca
     3901 tctatgagtt atctacaggt acgaaagatc agctgattat ggcgatgaga tttgcttacc
     3961 tctcattaga agctaaccgt tcgatatgcc cgatcatcat tgatgatggc tggcttcatt
     4021 atgatcatca gcgaaagtat caattagctg aacttttagc taaatttgga gaaaagtatc
     4081 aaataatttg tttttcttct gataaagaaa tggtaagcta ttaccatgag atgaaccaaa
     4141 tcgtaaaaca attagaagga gcaaataatg aaaaaaattc gtgatttagt aatcgatgag
     4201 cagtttgaac aatttgtcct gatcaaaagt gcagatgtaa gaatcgctaa aaacggaaaa
     4261 aaatttattg cttttacttt ccaagatact tcaggtacta tcgatggtaa atactgggat
     4321 gcttcggagg aggaaatcaa taaatttaca gccggcacag tcgtttttct aaacggtaaa
     4381 agagaggttt atcaaggaaa ccctcaagta aagattcttc atatgcgctt aacaagggta
     4441 gatgagccag gagagcctag tttatacatg gaaagagccc ctttgaagaa agaagaaatg
     4501 gaagaagaaa tcaatcaatt gttgtttgag atcaccaatg cgcattggaa tcgtattgtt
     4561 cgctatctac ttaataaata tcaaaaacaa ttttttgagt ttccggcagc aaaaagaaac
     4621 catcatgcat ttgccagtgg attagcttat catactttga caatgcttca cttagggaaa
     4681 tcaatcgccg atgaatatcc tgagctgaat cgagcattgt tgtatgctgg tatcattttg
     4741 catgatttag gaaaggtgat tgaactttcc ggtcctatgt cgacagaata tacgcttgca
     4801 ggaaatttga tcggtcatct ggtattgatt gacgaagaaa taactaaagc ctgtcttgcg
     4861 ttgaaaatca gcgaagctga cgaagatgtt ctcgtattac gtcatatggt tttagcacac
     4921 catggcttat tagaatatgg ctcacctgta cgtccaagaa taatggaagc agaaatatta
     4981 catcaaatcg acaatatcga tgcttcgatg cagatgatgt tgacttcgat tcgtcaaaca
     5041 gaaccaggaa aatacaccga tcggattttc ggtatggaca accgaagttt ctatgtacca
     5101 aaagatttat agcaaaaatt tatttttttc attcaaaaca aaaaaagcga gacataagtc
     5161 ttcgcttttt ttgttttc
//
LOCUS       DADVXO010000147         5159 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.152, whole genome shotgun sequence.
ACCESSION   DADVXO010000147 DADVXO010000000
VERSION     DADVXO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.152"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(53..1057)
                     /locus_tag="KB950_002527"
     CDS             complement(53..1057)
                     /locus_tag="KB950_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBB6858694.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1128..1424)
                     /locus_tag="KB950_002528"
     CDS             complement(1128..1424)
                     /locus_tag="KB950_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858695.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1417..1710)
                     /locus_tag="KB950_002529"
     CDS             complement(1417..1710)
                     /locus_tag="KB950_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858696.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1856..2407)
                     /locus_tag="KB950_002530"
     CDS             complement(1856..2407)
                     /locus_tag="KB950_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBB6858697.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2581..2880)
                     /locus_tag="KB950_002531"
     CDS             complement(2581..2880)
                     /locus_tag="KB950_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBB6858698.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2907..3215)
                     /locus_tag="KB950_002532"
     CDS             complement(2907..3215)
                     /locus_tag="KB950_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBB6858699.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3332..4291)
                     /locus_tag="KB950_002533"
     CDS             complement(3332..4291)
                     /locus_tag="KB950_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6858700.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4435..>5159
                     /locus_tag="KB950_002534"
     CDS             4435..>5159
                     /locus_tag="KB950_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6858701.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTEN"
BASE COUNT         1531 a          954 c          801 g         1873 t
ORIGIN      
        1 attaaataat ttttctcaaa atcagttttt ctttgataaa taatattttc aattatttaa
       61 tcatttgaaa attgttagtc attccaatac gttgagaaat tcttccatct gtataaacac
      121 ggaattgttt tccaccagcc attgttgtat ttccattatc atccgttaca atattaattg
      181 aatagtagct tccattttca tcttgcatta gatctttcat atattcattt gtgctaatta
      241 aattagtatt tgatgcattt ttatcaggag aatcatacaa ccataaagct ttattattta
      301 gtacagtttc tttagcagct tcccaacttg aaattgctga gctacttgta gattgtgtcg
      361 ttgtagttgt tgcttgttga gtagattctt ctactactgg agtttgagga gtttgtccat
      421 tcacaatatc aatgaaacct tgttttaact gttggttctg agtttcattg aagtacatgt
      481 agtttttatc attgccttga ttttgttgcg ttaaataaac tttaggttgt ccattttgaa
      541 agccaaagaa atatacatgc tgccctaaat attctccagt ttctacatca gaataaactg
      601 ctactaaatt gaaatctgca tgaccagttc catcttctga ccattgtacg gtaactggtg
      661 cttcattgat tgccattttc caatttccat caataactgc acttggtaaa ggcgtaccat
      721 acagacttac attgttctga ggattgtagc ttttatattc ttgacctaat gtctttcccc
      781 attgtgggac aaaaattgct agttgagtag atttctcact atcccacaaa gtatttttaa
      841 cctcttcaga agaagattca atgattgaac tttcacttgt acttgcttcc acagattgtt
      901 tcatttctgt tgtagtttgt tcagttgatt gcgttttact ttttttagcc tcaattgttg
      961 tatcagtttc tacagtctct ttagtcctcg tattctcaga tgtacaacca gtgaataaaa
     1021 tccccattcc aattagtaat aacaaccatt ttttcatttt atttccccct ttcaatttct
     1081 ccctcgcgta cttattttat cagtcaatta atctcaatgg tgttcattta gttaatcctt
     1141 tcccaagaat gtcgatagcc agcatgacaa cgatctcttc cttgagggct ttcagatgga
     1201 ttaaaactag tagaaatttc ccttccacat cctctacagc gataagttct cattattttt
     1261 gggctattag tattattttt cttagttaga gctatgagtc caactatgat taccccaact
     1321 gtcttaatta cttttccttt atctatattc ggatgcgcaa gcatatattt atataaccaa
     1381 tatccaccgt aaattaaacc acctaataca atcagcttag ccatgtactc gtctcctttc
     1441 tttttgttta attacttttt gaatccatct tgcagagaaa ccataaactt ctgtcaattg
     1501 acggatatct acagtttttt cctgttctat catgttttcg attctttttc taactaactc
     1561 acgatcgtac attttcattg gaaaagaaat ctgttgccct cgaaaagcaa gataaaattg
     1621 atagagcaat tctgaatcca gttccatttc ctcaaaaaag tcattcatac ttttataaga
     1681 gttactcaaa caagtttcat caatctccat ctacacaccc cctgttattt tttacataat
     1741 gaaaatagca tattactggg tacattgata attcatataa catgtatcat cacatgtatt
     1801 tttaaagtca aaaagcatgt aaaactactt ataagtaaca taagaaaata aaatgttatt
     1861 tttcttgaaa taataattga ctatctgcca attgccattc attacgaaca tgaatactta
     1921 gtgggtggga taacttacct tctgcattta tcaatacttt ggtcatcttt ttttgtttcg
     1981 cactcaattg atcgtaaatg gtcttcgctt gttcataagc cacttgactc gtcgctgtaa
     2041 tcgaaccaca agcaaaaata atggtgttga ttttcttttc tgtgaaaaca gttgttaata
     2101 tttccaaaga ttgttcatca tttccacgag gaaatacccg aggacttttc gcttttgtat
     2161 acgaaaaaag attcacagca ataaaaccag taaatcctaa ttgtcggata ttcttttcga
     2221 ttaataacat actcaaatca ttcgtaaatg gttctgtatc tgttggacga atagtaatga
     2281 ctaccgctaa tttatcttcc gcttcttttc ctttgatcgc ccattgtcgt ttgaaaacat
     2341 agcgatgggt atgatgagga tctcgccaaa cttctgtttg tgtggtcact aattcttttt
     2401 caatcatctc tttttctcct atgctaattt ttgttcaagc aattcaatca ccattgccgt
     2461 tataagtgtc aatactagtt gaaaatattt catattcttt ctcctttttt cttcagaatt
     2521 tgatgaatcg ctgtaaggat atcttctaaa catttcgcaa tcattctcaa ataatcgatc
     2581 ttattcattc gttcctccaa tctgactcca aaccaatcgt ccttctacca gtaacccacg
     2641 aacaaattct cgctggctgg taatatctgt aatgaaataa actacatagt cttttccatc
     2701 ttctggtgcg caataaatat aaagatgtgt catcactaaa cgtggtagag taacatcttc
     2761 aggaagcata cccatttcaa tttcctcttt ttccatcgta acggattcat agccccactc
     2821 ttcaggagta gtgactagct gtgcacactt ttccagtact tcgatagccg tttctaacaa
     2881 gtcctttcgt tcctttcttt tagatatcag tattcttctg gtaataacat cgtttgatac
     2941 tcacccgaat caatgaccca tattttttga gacactgatt tcaaacgaag cttaggtgaa
     3001 atcagatact cttttcttgc ttctggttct tcttgacgat gaaccaactt ttgatttcct
     3061 ttttccgtag aaaattcaaa gatttgaaga tagtctaaat gaaattgctt ttgaacgttt
     3121 tgatcgatta gattccaaca taaagcttgt aattccaccg gcaaactgtc cattactccc
     3181 tttgtgacat aacgttgtcc tttattttga aacatttctt ttccctcctt tgccataaaa
     3241 atagcccaat cttctattaa agaagattgg gcttcatgga tataatatat atttgatatt
     3301 caatttgatt cattttatac tatctgattt gtcaaattaa gctagacaat atgtctttat
     3361 ctatgcagct caaaaatact tccaatggtg ttcgataatt taatgatttt cttggaatat
     3421 ggtttctttt cgaggcaacg gattgaataa aggtttcatc cgtgtcccta aagtccattg
     3481 ttttaggtaa gccatctcgt ctaagcaagc cgtttgagtg ctcatttagc cctctttgcg
     3541 aaggtgttcc tggatctgcg aaaaagatgg caatatcgtt cgagttactt atagatcgcc
     3601 agttggaaaa ttcttttcca caatcaaatg tcatggattt aaacaagtgt ttaggcatgg
     3661 atttgaacca atcatttaac ctgttttcga tatcttcggc tcgtctgcct gtcggtttca
     3721 aagtaataat aaccttggat aaacactcaa ccaatgtaat aacagcgctc ttgtgtttaa
     3781 ctcccacaat ggtgtcgact tcgagatgac caaattcttt agtcaggtgt ggatagagac
     3841 tgttccgttc atgaatcgtt ctgcgaaagg cctgttttcc acgcttttct tgatgattat
     3901 tcggtttacg tttccccttc ataggtaacg actgtgtgtc aaaaaggcct tttttaaaca
     3961 tccgatagag tgtacgaaca gaacaatcaa ttggaaactc gccacggccg ataatcacat
     4021 cgggtgacca tccttgagct accttttctt gaacatatgt tgattgttca tcagataaaa
     4081 cggttgaacg tcgtccacaa cgagatttat tttgtttgta ttgacggtaa taatctagca
     4141 ctttttttcc ttgtttgaga aacgaataaa cattataaat ggtttgtcta gagcgattta
     4201 aactttgtgc gacaagagcg acaggtttct cgatattgaa ataagactct atgattacta
     4261 gttcgtctgt tgtaagatgg gtgtaagtca tttgtactca ctccttatta attctttggt
     4321 cggaactatt ttgagtgtag cacaaatgat ctttttagtt gtctagctta attttacaat
     4381 cgacgtttta ataaattgtg atataatgaa gtataacttt ttagggagtg tactatgaat
     4441 tttcaagata atagaccatt atatttacaa gtagaagctt ctatacgtaa cgatattcgt
     4501 gaaaaaaaat atcttccagg agagcaatta cctacagaag aagaattatg taaaatttat
     4561 ggagtaagta agattaccat tcgaaaagct tttaaacttt tagcagaaag tggaacagtt
     4621 gaacgattaa gggggaaagg gacctttgta actcaaaaaa aagaaagttt aaaattagct
     4681 ggagttcatg gatttagcga ttctctttct acccgaggac ataaaattag atatactgtt
     4741 ttacattcta aaattataaa ggctgatgag tttttagcta ataaattaca aatcaatgta
     4801 actgatagag tttacaatat taaacgttta atgtgggaag acggtgcccc gatgggaatt
     4861 gataattttt tcgcatcaga agaaaaattt ccaactttat ttgaaaaagg tagccaatat
     4921 acttcattat ataaacttct agaagatgac tatgatgtca aagctacgag ctctattatg
     4981 gaaataaacg gtttatctgc aacacctgag ctttccgact tattgcaatg tatagtagga
     5041 gatccgctat ttattttaga aaaggttggc aaagataaaa ataatcaacc atttcattat
     5101 tctcaatcca cagttcgttg tgatagaatt agttatgtta ttgaagtcac agaaaatat
//
LOCUS       DADVXO010000148         5074 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.153, whole genome shotgun sequence.
ACCESSION   DADVXO010000148 DADVXO010000000
VERSION     DADVXO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.153"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            52..1193
                     /locus_tag="KB950_002535"
                     /pseudo
     CDS             52..1193
                     /locus_tag="KB950_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1319..2398)
                     /locus_tag="KB950_002536"
     CDS             complement(1319..2398)
                     /locus_tag="KB950_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6858702.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2501..2941)
                     /locus_tag="KB950_002537"
     CDS             complement(2501..2941)
                     /locus_tag="KB950_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6858703.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2998..3408)
                     /locus_tag="KB950_002538"
     CDS             complement(2998..3408)
                     /locus_tag="KB950_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBB6858704.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3588..4022)
                     /locus_tag="KB950_002539"
     CDS             complement(3588..4022)
                     /locus_tag="KB950_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6858705.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4122..4970)
                     /locus_tag="KB950_002540"
     CDS             complement(4122..4970)
                     /locus_tag="KB950_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6858706.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1847 a          975 c          846 g         1406 t
ORIGIN      
        1 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
       61 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
      121 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
      181 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
      241 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
      301 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
      361 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
      421 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
      481 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
      541 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
      601 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
      661 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
      721 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
      781 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
      841 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
      901 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
      961 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     1021 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     1081 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     1141 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     1201 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     1261 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     1321 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     1381 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     1441 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     1501 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     1561 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     1621 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     1681 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     1741 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     1801 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     1861 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     1921 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     1981 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     2041 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     2101 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     2161 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     2221 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     2281 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     2341 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
     2401 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
     2461 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
     2521 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
     2581 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
     2641 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
     2701 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
     2761 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
     2821 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
     2881 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
     2941 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
     3001 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
     3061 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
     3121 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
     3181 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
     3241 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
     3301 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
     3361 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
     3421 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
     3481 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
     3541 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
     3601 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
     3661 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
     3721 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
     3781 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
     3841 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
     3901 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
     3961 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
     4021 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
     4081 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
     4141 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
     4201 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
     4261 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
     4321 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
     4381 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
     4441 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
     4501 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
     4561 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
     4621 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
     4681 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
     4741 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
     4801 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
     4861 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
     4921 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
     4981 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
     5041 ttaattaata aaaaaattag gctagtctaa atta
//
LOCUS       DADVXO010000149         4956 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.154, whole genome shotgun sequence.
ACCESSION   DADVXO010000149 DADVXO010000000
VERSION     DADVXO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.154"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(54..827)
                     /locus_tag="KB950_002541"
     CDS             complement(54..827)
                     /locus_tag="KB950_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6858707.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            971..1321
                     /locus_tag="KB950_002542"
     CDS             971..1321
                     /locus_tag="KB950_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBB6858708.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1302..2018
                     /locus_tag="KB950_002543"
     CDS             1302..2018
                     /locus_tag="KB950_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858709.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2018..3466
                     /locus_tag="KB950_002544"
     CDS             2018..3466
                     /locus_tag="KB950_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6858710.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="KB950_002545"
     CDS             complement(3537..4046)
                     /locus_tag="KB950_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858711.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="KB950_002546"
     CDS             complement(4036..4761)
                     /locus_tag="KB950_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858712.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1704 a          843 c          996 g         1413 t
ORIGIN      
        1 taagaaaaaa taagcgattt tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc
       61 agatcaaaat acttcaatgc gtggacaatt ccgccttctg tatttttctt tgtgataaag
      121 gtagaaagtt cttttaatcc gtcataggcg ttgcccatag cgattttgtt gtcgcaagct
      181 tccaacaagg caaaatcatt cggaccatcg ccaaatccaa aagtaggaac tttttccagc
      241 tgaagttctt tttgcagacg tttgacacct gccccttttg agattccttt cttcactgta
      301 tcgatcgagt aaggaccatt ccgataaaaa gttagctcag gaaaatgttc gtgatagtat
      361 tcgtcacctg attgagataa gatcagcaac atatttactg tgttttcttt aaagcctaaa
      421 ggatcaattt ccggaacatc tgaatgaatg aagtcgtaag cattgatcat tgtttggcta
      481 tgaccggtac accagatttt ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt
      541 acatgatcat acattttttg acattcttct gaagaaaatg tttgagaata gatttcttcg
      601 ccgtctactc gaatgaaaga accattcatg gtgaccgcag aagtaatgcc cgcatcttcc
      661 atgatagagt ggatttctgc ttctgtacgt cctgtagcga tgacggggag aacattgttt
      721 tcttttaatg cgttcatagc ttttgccact tcgggtgtga tttttgattt cgcatctagt
      781 aatgttccat caagatcaaa gaaagtgatt gcacgataat ttgacacaaa taaaactcct
      841 tttttattgg aataagttta tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag
      901 aattcctgaa catattggtt cagtcaacaa tttagagtat actataagaa gagaatttag
      961 gagggaaaga atgatttttt tcagtattgg tatcttattc ttaataattg gcagtctttt
     1021 tcggattctg ccctcaaaag gaaatctgcc tttttatggg tatcattcgc ctcttgccgc
     1081 aaaaacagac gctcattggc ggctggctca aaagacaagc ggaaactggt tttttcttat
     1141 gggcttactg atggcattga tcggctatta tttaaagaca tctgggcata cgaattattt
     1201 tctgattgaa atgcttctgc tagtatttcc aattatgcct atttttatta tgacagaaaa
     1261 gaaattacag aaatatgacc tggaaactgg agggaacgat aatgaatatt ttaatgattg
     1321 aagataatca atctgtttct gaaatgatgc agatgttttt cttaaatgaa ggttgggaag
     1381 caactttcaa gtatgatgga aaagaagggc tcgatgcctt tttagaaaac ccacaacatt
     1441 gggatatgat tacactagac ctgaatttgc ccacactgga cggtatgcta gtaggaagag
     1501 agatccgcaa agtttcaagt actgtgccga ttatcatgct gacagctaga gattctgaaa
     1561 gcgatcaggt gatcggctta gagatggggg cagatgatta tgtcacaaaa cctttcagtc
     1621 ctctgacgct tattgctcga atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa
     1681 gagaagaagc agtagtttca gatgatcatt ttgatatcga gactagtcat ttcaagatga
     1741 atacgaaaac acgagaagcg tatctggaca accggccaat tgaaggactg acgcctaaag
     1801 agttcgatct attgtttact ttagcaaaaa aaccacgtca agtattctct cgggagcagt
     1861 tgcttgaatt agtctgggat tatcagtact ttggtgatga acggacagtg gacgcccata
     1921 tcaagaaatt acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa actgtttggg
     1981 gagtcggcta caaattcgat gattctggtg ttgcctaatg cgttatttgt atcagcaact
     2041 cttagctttc tgcggaatgg tcgcgttgat cttattgatc gttggagttt cttttacaca
     2101 actgacaaaa caaacgattg aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc
     2161 agttgaaaag acaacgcaaa cctatgccga tacgctcccg cagtttagtc aagatcaggc
     2221 gtttcaaaat gcattgctgc tgacagagca agtactgaca gaacaaaatg tgaactttat
     2281 atttatcgat aaaaacgaac gtgtcattta tccgactaat acagctttac tgaattttac
     2341 cttaacagct gatcaatgga aaaatctgag gaatggacgt caagaaaaat tcacatcgaa
     2401 taaaaatatt ttaggacaag cacaggcgac ctcttacgca cttgtgccat ttaatttgaa
     2461 tcatgaattc tatggagcac ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt
     2521 tcgtccaatt acactgaacc tgttcaaagg atttattttt tccagtatcg ttgcagttat
     2581 tgccagctat gcttttgcag cattccaagt taagcgtatc aatcggttaa ggaatgcgac
     2641 gaaagaagtg acgaatggta attttgatgt acagctcccc gtacatgaca aagatgaatt
     2701 tgatgagtta gcagatgatt tcaataaaat gacgaattca ttgaaagaat ctcaagcaga
     2761 aattgaagaa caagaaaatc gtcgaagaca atttatggca gatgcttctc acgagatgag
     2821 aacacctctt acgacaatca acggacttct ggaagggtta gaatacaatg caatccctga
     2881 aaatcagcga gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt
     2941 ggtgaatgaa aacttggatt atgaaaaaat ccgtacgaat caaatcagta tggtcattaa
     3001 aaaattcaat ggaacagaaa cattgaaaaa tattgttgca cagttagaag ctaaagcaga
     3061 agcagcagga gatacattga cattgaaagc cgttgatgat attgatgttt acgctgatta
     3121 tgaccgattt gtccaaatca tggtcaatat tattcaaaac gcgatacagt ttactgaaaa
     3181 cggccaaatc acgattactt tagaaaaagg ctatcttgaa acgatcatca cgattgaaga
     3241 tacgggaatt ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt attataaagt
     3301 agatccttca agaaaaaata caaaatatgg tgaatcagga ttaggtttac caatcgtgca
     3361 gcaacttgta cgcttgcata aaggaaaatt agaagtagaa agcgaactag gaaaaggaac
     3421 aaaattcgct gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat
     3481 aaaagatcat gaatcgatct tctataccgc ttgtttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagcttacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggtgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgca aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DADVXO010000150         4887 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.155, whole genome shotgun sequence.
ACCESSION   DADVXO010000150 DADVXO010000000
VERSION     DADVXO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.155"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(406..780)
                     /locus_tag="KB950_002547"
     CDS             complement(406..780)
                     /locus_tag="KB950_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6858713.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="KB950_002548"
     CDS             complement(803..2203)
                     /locus_tag="KB950_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBB6858714.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="KB950_002549"
     CDS             complement(2207..2617)
                     /locus_tag="KB950_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6858715.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="KB950_002550"
     CDS             complement(2635..3126)
                     /locus_tag="KB950_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858716.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="KB950_002551"
     CDS             complement(3136..3927)
                     /locus_tag="KB950_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6858717.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="KB950_002552"
     CDS             complement(3920..4693)
                     /locus_tag="KB950_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858718.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1554 a          997 c          647 g         1689 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactc
//
LOCUS       DADVXO010000151         4850 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.156, whole genome shotgun sequence.
ACCESSION   DADVXO010000151 DADVXO010000000
VERSION     DADVXO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.156"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="KB950_002553"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="KB950_002553"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBB6858719.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="KB950_002554"
     CDS             complement(1297..2133)
                     /locus_tag="KB950_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6858720.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="KB950_002555"
     CDS             complement(2317..3534)
                     /locus_tag="KB950_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6858721.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="KB950_002556"
     CDS             complement(3683..3862)
                     /locus_tag="KB950_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858722.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="KB950_002557"
     tRNA            3917..4000
                     /locus_tag="KB950_002557"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="KB950_002558"
     CDS             complement(4240..4818)
                     /locus_tag="KB950_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858723.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1481 a         1008 c          780 g         1581 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat
//
LOCUS       DADVXO010000152         4801 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.157, whole genome shotgun sequence.
ACCESSION   DADVXO010000152 DADVXO010000000
VERSION     DADVXO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.157"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="KB950_002559"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="KB950_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBB6858724.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="KB950_002560"
     CDS             complement(2066..3064)
                     /locus_tag="KB950_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBB6858725.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="KB950_002561"
     CDS             complement(3290..3943)
                     /locus_tag="KB950_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBB6858726.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="KB950_002562"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="KB950_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBB6858727.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1410 a         1007 c          744 g         1640 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 a
//
LOCUS       DADVXO010000153         4789 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.158, whole genome shotgun sequence.
ACCESSION   DADVXO010000153 DADVXO010000000
VERSION     DADVXO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.158"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(100..1119)
                     /locus_tag="KB950_002563"
     CDS             complement(100..1119)
                     /locus_tag="KB950_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBB6858728.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="KB950_002564"
     CDS             complement(1116..1340)
                     /locus_tag="KB950_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBB6858729.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="KB950_002565"
     CDS             complement(1337..1630)
                     /locus_tag="KB950_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858730.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1667..1804)
                     /locus_tag="KB950_002566"
     CDS             complement(1667..1804)
                     /locus_tag="KB950_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBB6858731.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1806..2237)
                     /locus_tag="KB950_002567"
     CDS             complement(1806..2237)
                     /locus_tag="KB950_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858732.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2251..2745)
                     /locus_tag="KB950_002568"
     CDS             complement(2251..2745)
                     /locus_tag="KB950_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858733.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(2745..3194)
                     /locus_tag="KB950_002569"
     CDS             complement(2745..3194)
                     /locus_tag="KB950_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858734.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3198..3818)
                     /locus_tag="KB950_002570"
     CDS             complement(3198..3818)
                     /locus_tag="KB950_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858735.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3818..4726)
                     /locus_tag="KB950_002571"
     CDS             complement(3818..4726)
                     /locus_tag="KB950_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBB6858736.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
BASE COUNT         1301 a         1036 c          749 g         1703 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgctc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataatgga acgaaaacca
     1741 ttacttgttc gttagtgaaa cagccccagt catacattag tttgatatct tcatatgaat
     1801 acatgttact tcccaccttg taattgtttt ttgatttctt cgatttcctt ggtattttgt
     1861 acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac tttctgactt ttctttcaat
     1921 aagacatttt cttgcttgat tgctacattg tttagcattg ctttagaatt tagctgtttc
     1981 aattcatcat tttctgcctt cagcgtttcg tataatgcct taatattagc caattcatct
     2041 acgctatcaa cagcattccc ttttttctct tcttcagtag ctaactcaac ccactgtttt
     2101 ttatcaaaat caaatcttgg tctccagtta ggaactggtg gtttaatctc tgtacagttt
     2161 tcagggatat tttcttgatt gttcaaaatg atttgctcga atccgtaagg cttgattgat
     2221 ttgtaaacta ctttcattag atttcctccc ttaaattgaa taagtgataa caaatgaata
     2281 agctgatcca taacttgaat ttcttctcca tttaatggct ccgtctgcgc caatagatag
     2341 ttgagcactg ttcaaagtag aacggtctat tgacccaacc agttgttcaa aactaattgg
     2401 tgtccgatag ccttctggaa cagttaaaat aaccgaatca ttcccgccac tactttttcc
     2461 gtttaaagcc acaaaatata tagaaactgt ttttccttca cgataaagct ttgctgtccc
     2521 tgtattaccg ttagtgacaa ccagatttac cacttcatat ctgttatcat cagtcgtcaa
     2581 aacacgttta ttattgattg ttacagaacc tgaaaaattc ttatgtcctg caatggcttg
     2641 gtctccatct gtctgcacca atgcttcttt accatcaata gcttctacat gcgtttttaa
     2701 gtatttagca actccgtctt cttttaattg aacaatatca gccattaaac cgtccccact
     2761 ttctcaaatg taatattcgc taatccatca agtttcactt tatcggttgc tgacattaaa
     2821 cctgctgtcg tagttgtagc attacctgga tttttctgtg ctccagctgc aattccatcc
     2881 aacttagttt tatctgtgga cgacatcaac ccatttgctg tagttgtagc tacagctgta
     2941 gttgtggcat ttattccagg atcaccttta tctccctttg gtaaaacaaa attaaatcta
     3001 gctgcagatg atgttcctac attcgtaaca gaagcggttg aaccactaga aacggttcct
     3061 atggtaattg ttgctgcttg gccaggatcg cctttatctc ccttcaccgt tgttggtttg
     3121 ccttctatag cattccaatg agtttgtgga taaacctgta cattgctttg ttttattttt
     3181 acgatatctg tcattctcta tacctcccca attttttcaa aagtaaaatt tggaattctt
     3241 tcgtttgtgt aattttctgc ttgatttaca gcttcttgga atttttgatc tacatatgac
     3301 tgattgacac caccagtccc actaccacct gtagaactaa ttgttccatc ttcatcaata
     3361 gtgatgtttt ctccagcagt ataatttttc agtgaatcga gtttttcttt taactccagc
     3421 gaaaaattga catcagattg tctgactgca gagactttcc ctgtctcatc aatcgtcaat
     3481 aattcaccaa cttttatgcc acccaaacgg tcgatagtgg caactggcaa cacgtagtca
     3541 ccttctccac tgtttaaaat tttttgatac atttctgccg tgataatgcc gtcatcttct
     3601 tcacttgcat agggtaattc tgtgagaaca ttctctaatc ccaaatcttc tttagtaatg
     3661 acgacggctc caatttttcc attaacagat aaaactgttg cttctcctgc tattatttct
     3721 tctaacccaa gaactgcgga agcatgtgtg attggaaaga attggcgttg tacacccgtt
     3781 ttttcatccg tttccatcat tcgtttagtt ttagccatta gatcacccca actttctcaa
     3841 gagtgaacac attttgcttt ggatcatcaa cggttgcgat gatcaaggct ccttcttcaa
     3901 tcgggaatgc tacagttcca actttttcga cttcatgatt atctgaaaag agatcatcct
     3961 gcaaaatatc agtcacttca atctccccgt attcaatcgt gaaaagcgtt gctcgcaatt
     4021 tctgatataa atagtccata tctgccagca aacgctctga aatagacgca tgacgaactc
     4081 cttgaatatc tactcgcgca tccgttagtt cggctaacat gactccacct ggatcaatcg
     4141 tctttaaaat atccttgatt gattcaaacc atgccaaata gtctgattct tgtccttctc
     4201 tccaatcttg gaaactgtgt tcctgttctt gtctccaacg ttcaaattct tctttccttt
     4261 cattcatcca gtccgtaaaa tcgcctttat tttcattaat aaagtccgtc atatccgcaa
     4321 ttaaatcttc gattgattgc caataagaac ccatttctcc ttctgttttt gaaacagcat
     4381 taatgacaaa ataagagaag tcttgagttg ttccaatcaa gtcttctcct ttaaatataa
     4441 tgaaattggc tgtttgtcga tgtaaacact gcatggaata tttatcaaaa atatagttaa
     4501 ttttcccttt tttagcatcc acaatttttg tttctaattg gactggatat ttcccaccta
     4561 caactgattc aaaatatacc ttacattctg ataaatcgta gggaagacca ttttcaacta
     4621 tagtcgcttc cataacctct gtattcttat tgccttgtcg aacttgaatc atccccacgt
     4681 aattataggg ttctgttgta cttaatatga cattccactt tgccattaaa tcacctccct
     4741 attttggtgg tatgacaatg gaagaaattg cacttgcact ataatactg
//
LOCUS       DADVXO010000154         4736 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.159, whole genome shotgun sequence.
ACCESSION   DADVXO010000154 DADVXO010000000
VERSION     DADVXO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.159"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..611)
                     /locus_tag="KB950_002572"
     CDS             complement(<1..611)
                     /locus_tag="KB950_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858737.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQ"
     gene            complement(759..1301)
                     /locus_tag="KB950_002573"
     CDS             complement(759..1301)
                     /locus_tag="KB950_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBB6858738.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1436..1825
                     /locus_tag="KB950_002574"
     CDS             1436..1825
                     /locus_tag="KB950_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858739.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1829..2593
                     /locus_tag="KB950_002575"
     CDS             1829..2593
                     /locus_tag="KB950_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6858740.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2722..3669
                     /locus_tag="KB950_002576"
     CDS             2722..3669
                     /locus_tag="KB950_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6858741.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3799..4539
                     /locus_tag="KB950_002577"
     CDS             3799..4539
                     /locus_tag="KB950_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858742.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1646 a          783 c          943 g         1364 t
ORIGIN      
        1 ttttgtttgt tttttctttg ccacataaat acacctagac tgacgatgaa gagcatatcc
       61 aacagatttc ctgcgacttg gatcgtcgaa gggttggtca gtggcattaa agcaaggaca
      121 aaaaaggaaa acaagctgta aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga
      181 catttcaatt ggctaaaaat gagtgaaaaa ataaaataag taaaaatgga taaaatcacg
      241 actaaaccat tcatgataaa gaagaaaagc gtattgtgat taaacgaata aaaaagaatt
      301 gctaaaaaag aaaacgtttt aaatgcaatc aatacgcttg aagcttcgtt taacgtacca
      361 ggatgtcgat gaaaaaaata ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa
      421 gacaaaatca aaaaaaacgg attttcttcg gcttctgtga tatcagaatg atccaaaagc
      481 aaccagccta atgcactaga aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa
      541 aataagatcg tcaaagttgc aaaaaaagag cctaaatgta aaaaattagt ggttctacgt
      601 tgaatttcca taatatctcc aaattaaatt gataagtata taaagtataa taacaaattg
      661 atgagtatat gtcgatgttt tttaatcaaa ataataaata aaaactgtct aacatttaaa
      721 atttaattta gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc
      781 acttttttag tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca
      841 cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt
      901 atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa
      961 ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc
     1021 gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata
     1081 cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa
     1141 acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc
     1201 agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa
     1261 ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt
     1321 tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac
     1381 aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa
     1441 aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt
     1501 aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct
     1561 gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt
     1621 cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg
     1681 agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt
     1741 atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg
     1801 aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca
     1861 tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt
     1921 catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat
     1981 tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca
     2041 gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga
     2101 atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt
     2161 tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat
     2221 agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt
     2281 aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg
     2341 ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt
     2401 ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa
     2461 aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca
     2521 tatggtacaa atacaactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa
     2581 aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta
     2641 ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa
     2701 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
     2761 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
     2821 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
     2881 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
     2941 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
     3001 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
     3061 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
     3121 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
     3181 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
     3241 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
     3301 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
     3361 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
     3421 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
     3481 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
     3541 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
     3601 gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca
     3661 acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt ttcttaaaat
     3721 aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa agttccattt
     3781 ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt tcctgcgtac
     3841 gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt tgacggacaa
     3901 gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga atttcatttc
     3961 acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca
     4021 gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta taccggccat
     4081 taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc ctctcgttct
     4141 gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc atctgttgac
     4201 acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc gattatccca
     4261 gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa aaaaacggat
     4321 gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag aaatggtgag
     4381 ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa
     4441 aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt gatcgatgat
     4501 caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa atttatattc
     4561 ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta tccgaaagtt
     4621 tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa taagtaagaa
     4681 aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa atagtt
//
LOCUS       DADVXO010000155         4724 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.160, whole genome shotgun sequence.
ACCESSION   DADVXO010000155 DADVXO010000000
VERSION     DADVXO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.160"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(207..1169)
                     /locus_tag="KB950_002578"
     CDS             complement(207..1169)
                     /locus_tag="KB950_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6858743.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="KB950_002579"
     CDS             complement(1171..2610)
                     /locus_tag="KB950_002579"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBB6858744.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..>4724
                     /locus_tag="KB950_002580"
     CDS             2795..>4724
                     /locus_tag="KB950_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBB6858745.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTD"
BASE COUNT         1497 a          812 c          784 g         1631 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatt
//
LOCUS       DADVXO010000156         4684 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.162, whole genome shotgun sequence.
ACCESSION   DADVXO010000156 DADVXO010000000
VERSION     DADVXO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.162"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(1014..1304)
                     /locus_tag="KB950_002581"
     CDS             complement(1014..1304)
                     /locus_tag="KB950_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858746.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="KB950_002582"
     CDS             1706..1942
                     /locus_tag="KB950_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858747.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="KB950_002583"
     CDS             1949..3901
                     /locus_tag="KB950_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBB6858748.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="KB950_002584"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="KB950_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBB6858749.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1605 a          816 c          764 g         1499 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atga
//
LOCUS       DADVXO010000157         4638 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.163, whole genome shotgun sequence.
ACCESSION   DADVXO010000157 DADVXO010000000
VERSION     DADVXO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.163"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..379
                     /locus_tag="KB950_002585"
     CDS             <1..379
                     /locus_tag="KB950_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858750.1"
                     /translation="IHLLLETERWCSIEELQNELKVSSKSILNYLTDLEELFQKYPDK
                     VILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQHPEKNLEDYADAQYT
                     SVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(545..1738)
                     /locus_tag="KB950_002586"
     CDS             complement(545..1738)
                     /locus_tag="KB950_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6858751.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            1962..2339
                     /locus_tag="KB950_002587"
     CDS             1962..2339
                     /locus_tag="KB950_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6858752.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2327..2665
                     /locus_tag="KB950_002588"
     CDS             2327..2665
                     /locus_tag="KB950_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6858753.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2799..3242
                     /locus_tag="KB950_002589"
     CDS             2799..3242
                     /locus_tag="KB950_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBB6858754.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3504..4319
                     /locus_tag="KB950_002590"
     CDS             3504..4319
                     /locus_tag="KB950_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6858755.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4329..4544
                     /locus_tag="KB950_002591"
     CDS             4329..4544
                     /locus_tag="KB950_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858756.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIESIKYFV"
BASE COUNT         1587 a          824 c          884 g         1343 t
ORIGIN      
        1 gattcattta ctgcttgaaa cagaacgttg gtgttcaata gaagaactac aaaacgaatt
       61 gaaggtatca agtaagtcta tcttgaatta tctaacagat ttagaagaat tgttccaaaa
      121 atatcctgat aaggtcattt tgaagaatga gaataatcgg cgcttttcta tggaaaagca
      181 ggagaacttc ccaatctaca ttatttatct tcatttctac agaaaatcat ataattatca
      241 tttgattgaa ttcatgtatc aacatcctga gaaaaatttg gaagattatg cagacgctca
      301 atatacgagt gtatccacag tttttcgtta tgctaagtta ttagtaaagt actttgaaat
      361 gacggacgtc aaagaatagt cgccgccaga tagaaggaaa ttagcagttg aaaaagctgc
      421 cctgcatcaa gtaatacgac cagccaaacg gaaacagtgt ctcatgtttt tgatttggct
      481 ggtcttatta ccgcagatgc aagcttttgc cacacctctt tcttgtcatt tacagactca
      541 actatcaatg ctgcaagctt tcccttaatg ctgtaacacc agtaagagtt gtcaagacca
      601 gcagactgac taggtaaata ccgctcaaaa acaagattac ggcagttaaa ctataatgat
      661 ccatcaagaa accgatgacg atagaagaaa aaccaccaac agctcgtcca aggttcataa
      721 tgaaattatt cgccgtcgcg cggatttctg ttggatacaa actactaatc actgcgccat
      781 atccaccata catgccattg ataaagtagc cgactatcac agcggctgct accaaactcc
      841 actggctatg ggctaacaca agtagataga cagataagga tgaacaaatt aaaaacaaag
      901 aaaaggctag tttaggcccc aatttatcca tgattatgcc aaaagtcatc atccccaaag
      961 acatgcctaa aatagtactg accatccaca atgaagaccc agaaaccgaa agccctaatc
     1021 gattttgcat aattgaaggc agccaattca ttaacccaaa atagccggca atttggacag
     1081 ttaccatgat actaaggcca catgtttgcc aagcaagatg ttttgattca aacagtctgc
     1141 taagtttggt tttcttttcg gcagattgta cagatttgaa ttcctttggt tcagtcaagc
     1201 cgcgacgaat gaacaagacc atcaacaccg gcagtacacc tacgacatac aacatcttcc
     1261 aaccaaacgt tggaatgatc acagcagcta aaatcgcagc caaagccgca ccgacctgcg
     1321 caccaatacc agcaaccgaa gaagctcgtc caatttgttt ttttgaaaaa ctttcggaaa
     1381 tcaaggacat acacgccccg tattctcctc ccgctcctac acctgcgata aagcgaaaga
     1441 gataaaccaa gtagatgttt gaagcaaaat acatcaataa agaagcaatc gaaaacagaa
     1501 ggaccgtttg agaaaatact ttcacacgtc catatttatc agctatgatg ccaaaaaaaa
     1561 tacctccaat gagcatcccc aaattagtga tcgttgaaat gaaacccgct tgcgtaccac
     1621 taatatgaaa agaagtaatc attgaagata atgaaaaagc taaaaacata atatccatat
     1681 tctctaaagc aattcccgcc gatgtcgata ataatacctt tcgctgatac gttgtcattt
     1741 ttatacccct tttcctaaaa tttccctaaa ttgttaaaag tatatccaaa aaagaataaa
     1801 aaaacaagac ttttttattt gaaataatac acaaacaacc gatatagata atccagctta
     1861 gttttaatag aaatgagtat tgacttggag tacacttaaa gttgtagaat aaataaaata
     1921 cataagtaaa gtggcaagaa attaagagaa agagtgatca gatgaatatc aaacaagtca
     1981 gtgaagaaaa gggcatttcg gccgatacat tgcgctatta tgaacgaatc gggttgattc
     2041 caccagttaa tcggacaaaa ggcggaatcc gtgattatac agaagaagac ctaaaatggg
     2101 tcgactttac gatctgtatg cgaaatgccg gattatcgat cgagtctttg attgaatata
     2161 ttcgcctatc tagcgaagga gaagcaactg tatttgagcg gcgtaagctt ttgattgaag
     2221 aaagtgaact tttgacgaaa gaaattgctg aaatgcaaga gtgtttggaa cgtttacaag
     2281 gaaaaatcaa gaaatatgat cgtgtgttat ctagaaataa aatggaatgt atcaagtaaa
     2341 aaaatcaagg agcatcgcta tggagttacg cttactaaaa tatttttgga caattgcaga
     2401 agaaggaaca atctctcaag ccgcagaagt tctacatctt acacaaccaa ccttaagccg
     2461 acaactgaag gagttagaag aggaactagg aacagaactg tttgtacgcg aaaaccgcaa
     2521 aatgatcttg acagaagcag gttattttct aaaaaagccg agcagaagaa attttagatt
     2581 taacgaatca aaccactcaa gaattcgaga acagaaaaaa acaattattc agcggccgat
     2641 ttacgatcgg ctgtgtggaa gctgataatt cgaatacatt ggcactatta ctagaaaagt
     2701 tgatccgtga ttatcccagc gttcaatttc atatccacag cggaacaagt gatgatatca
     2761 cggagtgact agaaaaaagc ctgttagatt tagcgatttt gctagatcca gtagatacag
     2821 agaaatacga aacgattttg ttaccaagaa aggaaaaatg gggattgctc attttagaaa
     2881 attctgtttt agcaaaaaaa gacgtgataa caaaagaaga ctttgcgaat gtacctcttt
     2941 tgatctcaaa gcgtccagaa atacagcgaa tgtttgctga ctggttaggc ataaaaaagg
     3001 agcagttgct gatcacagga acgtacaatt tgattttcaa tgtatttgcg ctaattgaga
     3061 atcaggtagg tgctgcttta gtcatagaag gtgctgtaag tcatctttca aagcgagtga
     3121 cattcgttcc gcttgaccca atgattgaaa cgaactgcat gctagtctgg aaaaagaatc
     3181 ggattttatc acctgtagtc aatgaattga ttcaccgttt caaagaagct tttgaacagt
     3241 aataagtatc tctaatcgaa gaaatttcaa tacaaaagcg agacgtatga cagtcttgct
     3301 tttgtatttt ttcattttta aatcaaaaaa aataaagaga tttacataac ttgttaaata
     3361 agcgcttaca aacataagaa gaacggaaat gaatcagcta taataaccgt tgttctggat
     3421 tgtaatcatt acaaagaaat gaagattata actttggagg tgttcgaaat gaaagaaaaa
     3481 aagcaaaaat tcgttcaaaa aacatgccct ttattcaatg gttgggtatg attctgctga
     3541 ttgtattagc tttattgacg atcattcctt ttttatggat ggtttctgct tcattcaaag
     3601 caaataatga agtgttcacg attccgattc aatggatccc taaaacttgg catttagaga
     3661 attatctggt tatttggtct cgtattcctt tagtgacatt ttttaagaat acagcttttt
     3721 taagcgttgt gatcacgttg attcagttgt ttacttctag ttttgctgct tacgggtttt
     3781 ctaaactaaa ttttaaaggg cgcgatattt tatttttagc atatatcggc acgattgctg
     3841 tcccttggca gtcttatatg atcccgcagt tcattatgat gagaaaagtg ggtttaacat
     3901 atacgttgtg gtcgttggtg ctattacaag cgttcaatgc ttttggtgtg ttcttgttga
     3961 agcaatacta cagtagtatc ccagattctt tatgtgagag tgcgcggatc gatggcttat
     4021 cagagtgggg aatctatcgc aagatcattt tacctcttac aaaaccggcg attgccagtt
     4081 tgacgatcat cacattcgtt aatacatgga acgattatat ggggccattc atttatttgt
     4141 cttctacgga aaataaaaca atccagttag ggttgaaaat gtttattggg ctatttgatg
     4201 cagagtacgc attaatcatg gctgcctcag ttgtttctgt tcttcctgtt gcttttatat
     4261 ttttagcgat gcaaaaatat tttgtagaag ggatcgcttc atcaggaatg aaggaatagg
     4321 aggtgattat gaaagagcag cgttttgaca aaaatgttta tatggaattc ttttattggt
     4381 tgtatcaatc attggcaagt agcctttgtt tttggtgtgt caatatcttg tatatagcag
     4441 ctgcgttcct tttgtcgata gatggaagga atgtcttttc ctttattttt tctttacttt
     4501 ttattggtcc aggttgcatc gagagtataa aatattttgt gtaaatgaaa aaatccatac
     4561 aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga
     4621 cttccctatg aatgattt
//
LOCUS       DADVXO010000158         4620 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.164, whole genome shotgun sequence.
ACCESSION   DADVXO010000158 DADVXO010000000
VERSION     DADVXO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.164"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            153..959
                     /locus_tag="KB950_002592"
     CDS             153..959
                     /locus_tag="KB950_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBB6858757.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1684
                     /locus_tag="KB950_002593"
     CDS             977..1684
                     /locus_tag="KB950_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBB6858758.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1681..2652
                     /locus_tag="KB950_002594"
     CDS             1681..2652
                     /locus_tag="KB950_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBB6858759.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2645..3673
                     /gene="vanB"
                     /locus_tag="KB950_002595"
     CDS             2645..3673
                     /gene="vanB"
                     /locus_tag="KB950_002595"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBB6858760.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3679..4287
                     /locus_tag="KB950_002596"
     CDS             3679..4287
                     /locus_tag="KB950_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBB6858761.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4353..4526)
                     /locus_tag="KB950_002597"
     CDS             complement(4353..4526)
                     /locus_tag="KB950_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858762.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1300 a          972 c         1230 g         1118 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggttgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1321 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1381 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1441 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1501 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1561 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1621 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1681 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1741 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1801 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1861 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     1921 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     1981 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2041 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2101 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2161 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2221 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2281 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2341 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2401 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2461 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2521 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2581 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2641 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2701 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2761 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2821 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     2881 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     2941 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3001 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3061 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3121 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3181 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3241 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3301 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3361 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3421 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3481 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3541 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3601 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3661 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3721 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3781 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3841 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     3901 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     3961 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4021 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4081 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4141 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4201 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4261 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4321 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4381 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4441 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4501 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4561 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DADVXO010000159         4610 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.165, whole genome shotgun sequence.
ACCESSION   DADVXO010000159 DADVXO010000000
VERSION     DADVXO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.165"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            138..2078
                     /locus_tag="KB950_002598"
     CDS             138..2078
                     /locus_tag="KB950_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBB6858763.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2165..4087
                     /locus_tag="KB950_002599"
     CDS             2165..4087
                     /locus_tag="KB950_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBB6858764.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4367..>4610)
                     /locus_tag="KB950_002600"
     CDS             complement(4367..>4610)
                     /locus_tag="KB950_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBB6858765.1"
                     /translation="QTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKD
                     PAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1529 a          782 c          943 g         1356 t
ORIGIN      
        1 aacataataa atcgtatatc aattttttta gcgaaatttc tatttattcc aagaatttca
       61 gttattacgg aatcattttg tgcttaataa aaagaaaccg tttttatata ctataaatgt
      121 agaaaggcgg taggaaaatg aaaaaaatta ttgcaatcac atcctgtcca gttggtattg
      181 cccatacata catggcagca gaaaacttag agaaagcggg gaaagcagtt ggcgccgagg
      241 taaaagtcga aacacatggt tccatcggta ttgaaaatga attgactgca agagatatcg
      301 aagaagcagc tggtgtaatc atcgcagcag atactaaaat agataaatca cgattcggcg
      361 gtaaaccttt gataaccgta ggtgtgcaag aaggaatcca ccatgcagat gagttggtga
      421 gagatatttt agcaggaaaa gcgccagtct ataaaagtac ggaggcaatt atttcttccg
      481 aaaataaaag tgaatcagaa aatttaggta aaaaaatcta taagagtttg atgaatggcg
      541 tttcgtacat ggtacctttc gtagttacag gagggttatt gatcgcgatt tctttaacat
      601 taggaggaac agctacacct gaagggatca agataccaga aggaacgatt tgggcaacga
      661 tgaatagtat cggaagtatc gctatggggt tgatggttcc aattctatct gcatttattg
      721 cgcaaagtat tgcagatcgt ccaggacttg ttcctggatt tgttggtggg atgttagcgg
      781 caaacggagc attatatggt agtgatgcga atgcgggatt cttaggcggg atcatcactg
      841 gatttttagc aggattcatt gctttaggaa tcaagaaagt gcgagtaccg aaagctttac
      901 aatcgatcat gccaattatt gttatcccaa ttttaggatc attggcagtt ggttttgtgt
      961 ttatctatgt tattggagaa ccagtagctc ttctatttag ttcattgact cactttttag
     1021 cagggatgca aggaggaagt gaaattgttc tagctatgat cttaggagcg atgattgctt
     1081 ttgatatggg ggggccgttc aataaagtag cctttttgtt cggatctggc ttgattgcag
     1141 aaggcaatta ttccattatg gggccaattg ctgtagctat ctgtatccct ccattagcat
     1201 tggggctggc atcattgatt ttaaaaacca aatttacgaa aacagaacga gaagctggta
     1261 aggcgtcctt agcaatggga ctgtttggga ttacagaagg cgctattccg tttgcttcaa
     1321 cagatccact acgggtgatt ccaagcattg taatcggggc aatgacagga agtgtgatcg
     1381 cgatgctttc aggagtgaca gatcatgtag ctcatggcgg tccgatcgta gcggttttag
     1441 gagcagtgga tcacgtagcc atgttttttg tagcagtgat tgctggtatt gctgtaaccg
     1501 tgctaatgtt aagaatcctt aaaccgacat tacaacaaac tgcggttgct gcagcaaatg
     1561 gtgtgacagt agcagatcta tcagagactg aaacaagtga tgtttatacg aaaactgaaa
     1621 cttttgattc tatcacggat attatgtcag atgaagcaat cgttttaggt gaaacagcaa
     1681 caacaaaaga cggagtaatc gaagatttag tccaacgttt agaaaaaaca gcggcgatca
     1741 cagataaaac cagatttaaa gaagtcatct atgatagaga acatgaaagt acaacgggaa
     1801 tcggtatggg gattgctatt ccacatggaa aatcagctta tgttcttaaa ccaacagtgg
     1861 catttgccag aagtcaatct ggggtagatt ggcattcttt agatggaaaa cctgcacata
     1921 tgatctttat gattgctgta cctgaaaaca gccaaggtga tatgcacttg aaaatactgc
     1981 aacgtctatc acgtaaattg atggatgatg acttccgtca agcactgatg gaagcgccag
     2041 ataaaacaac agttcaccaa ttattaagcg aaatgtaaca taagatgaaa aatcgaccgt
     2101 gatgagacat aatagatgtg ttatcatgat cgatttttta tttagaaggg agtttctgca
     2161 cattatgaat cagcgtcagc aatctttgat ctatcaatta agtgagaaca ataattatat
     2221 aacaacaaaa aagctagcag agcatttcca tgtgtcagaa agaacgattc atagcgattt
     2281 gaataccatt gaaacttggt tgagagacaa acatctgcct ttaatgtttg aaagaaaaaa
     2341 aggtacaggt atcttgttga atggtctgcc tcacgaaagg gaacaactga aacgagcgct
     2401 taatgaagag actcaaacag aaatggataa aaaacagctt cgtcagttat tgattttcca
     2461 tctgctagta gcaaaagacg gatttagtct tgaagaactt tcagaaaaac tatatgttgg
     2521 aaaacgtacg attcgtaaag aattgacaga gttgcaaagc ttttttgagt atcaccattt
     2581 acagctagta tccaaaacaa aactaggaac ttttcttaaa ggcgatgaac aagaaaaaag
     2641 acagttgctt gttaagacat tacgaaatat gcagaaagaa gatccacaaa gtccgtcgtt
     2701 gaaagaattc ttcgctaaag atacattaaa agttatccaa catacattac aagatgtttt
     2761 ttatgaaaac catttagaac agccgaatgg actggctagt gtggagattc acatctactt
     2821 tatgctcgag cgaatgaaac agtaccagaa agtgaaatta agtaaagacg aaaacgaagt
     2881 cgtggaacac acccaagcac aaaagttaag tagtcaaata ttagcaaaac tagccaccat
     2941 ctatccaatt gaattttctc ctgatgaaat caattatctg gctttgcgta tcgccaatct
     3001 ttttacaaat tcacaagcag ccacacgttt tcaaaaggaa tctagcgcac ttgctgatca
     3061 tttgatcgaa caagtggaac agtttctagg ctattcatta aaagaagatc agctactaaa
     3121 acaaaattta aggtctcatt tatcttcaac ttattttcga ctccattatg gtttgcagat
     3181 ttctaatcca ttgacgaaaa atgtgtttag tacgtacaca caacttttct tagttttaca
     3241 gttaatttta gaagattatt ttgaaaagga aaatttttat gttccacaag aggagacagc
     3301 ttatttaacg atccatttcc aagcagcgat cgagcgacac aaacatcaga aaagtagaag
     3361 atatcaaacg gttttagtta gtgaatattc aaaagctatg gctacttttc tcgaagcacg
     3421 gctgaacaga gaacttccag aattaacgat tatggatcta gttgaatata agccagaatt
     3481 aaagagacaa gattttccct ctgttgattt tatcttggct acgttaccat ttaaacatat
     3541 aagcattcca gtaatcgaga tctcaccaat gatcacagaa actgatctag cctatttgac
     3601 aaaatatatg ctggagcatg tgcctatcaa gaagaaaaaa acatttgatc tagcaagctt
     3661 tactcatcca tttctgatct ttcctcaact tgaacggaca gacccagccg atattttaaa
     3721 ctttatggga aacgtcttag tggaacatca ttatgtggag ccggaattca tcgattctgt
     3781 cttggaaaga gacagtcact ccagtactag agtagcacca tttgtaacaa tccctcacgg
     3841 taatccgctg tatgtgaagc attcgatgat ttctattgct acaatgaaag aaccagtcct
     3901 atggcacggg gaacagatac gtatcgtact gctattagca ataaagaaaa atgatttgaa
     3961 acatgcagag ttcaaaaaaa tattttctgt tattcactat ctagaaaagc agccagaaca
     4021 aatggctcag ttgatgcaat ccaatcatcc gctagaaatc ttaacgttgt tatctagtta
     4081 tgaataagta gcgattaaaa aactgccttt atatttataa taattctatc tatttatttg
     4141 gaagtagaaa actaaataca aaaaaagagg atgtggccaa agttgtggca catcctttta
     4201 ttttgagttt cttataattt tttatagttg cttacgatgt cttgactact ggtaaacggt
     4261 gttcaagaga caatttgtcg gcaataaggc acaattttgt aaaatatgga aagctatttt
     4321 tttaacattg tttcttagtg cttgaaaaat cattttgaat acttctttat acgatcgatg
     4381 tacgttgatt taagtctaaa acttttcttt tgattccttc tctgactttt ctgaattccg
     4441 ttaatatttc ttcttcgctc ccgacagctt gtgcaggatc ttttaggtcc cagtgaaggt
     4501 gttcgtgatt gtttggaacg gcagggcact tgtctttggc atcaccacaa agtgtaatga
     4561 tcaagtctgc ctgagtaaag taatctctat caatcaaatc agacgtctgt
//
LOCUS       DADVXO010000160         4545 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.166, whole genome shotgun sequence.
ACCESSION   DADVXO010000160 DADVXO010000000
VERSION     DADVXO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.166"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(268..993)
                     /locus_tag="KB950_002601"
     CDS             complement(268..993)
                     /locus_tag="KB950_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858766.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1147..1473)
                     /locus_tag="KB950_002602"
     CDS             complement(1147..1473)
                     /locus_tag="KB950_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858767.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1510..1875)
                     /locus_tag="KB950_002603"
     CDS             complement(1510..1875)
                     /locus_tag="KB950_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBB6858768.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1912..2127)
                     /locus_tag="KB950_002604"
     CDS             complement(1912..2127)
                     /locus_tag="KB950_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6858769.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2256..2591
                     /locus_tag="KB950_002605"
     CDS             2256..2591
                     /locus_tag="KB950_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6858770.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2667..4217)
                     /locus_tag="KB950_002606"
     CDS             complement(2667..4217)
                     /locus_tag="KB950_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858771.1"
                     /translation="MVAVYEYLKKNIPGCDDNVNASNRMFFGGYKSDAVIIINEDNML
                     DITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSD
                     MSFNEIYERLLTIDMNKLLKSSKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDI
                     SENFIGVVMLILKKDSRVETFNWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFR
                     TIRNYLEEMRRVYDVLLSDVNFFDDSKESVTCILSGEQLAKKMSSYYGKTYDIDKCNK
                     LLSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFKHKRSNVYRLDVFNATSVI
                     QKLNEDILPLLEKFHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEA
                     HEVLQGLSWQGTYLMTEKELINTVCERLRNVRDNSVKNALIENRGYFVKQGFTLERAS
                     KDIKKLYNVKASSSFLVYLAKGAGIDEVIKELILSGVELVPIYQFTSVTRDDGRRVRN
                     RRDPIILN"
BASE COUNT         1435 a          894 c          597 g         1619 t
ORIGIN      
        1 aaaaaaacag tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta
       61 aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc
      121 gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca
      181 gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt
      241 aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta
      301 atcagttacc tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa
      361 ccgaagtgga atatctttac caactttctc tgtcactgca ttactgatac ctgttagatt
      421 agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt
      481 gattccttta tcttcattta caagtttcca agtaggtggt atagctgtgt atgtttctac
      541 aaatgaatta agtttttcat taagtgactt ctcttttata tttccatcta aaaactctgt
      601 atatatggct tgagctatac taaaacaaag ctggccttgt tctaataatt ttacatcatt
      661 agttagtaaa gctgtgttca cgttatcaat cgcttcaaga agagtatctt tggtgtccct
      721 aacagcttta ggtgtgcctt ctggaatttc cctacttgat acctcgtcca ttttatcttc
      781 cataaacccc ttaaaatcat atctttctcc caaatccata gaagtttttg agaaagcttt
      841 catttctgag tctatttctt caataatatt taacatgtct gatacatatt gtttaggctg
      901 attcgttttt aatctatatg tggtaaatat ctcaattcct ccaattatca ataaaattgc
      961 caaacaaatc cataataatt ttttcttttt catttttcct cctatacact gctgttataa
     1021 cagtgactaa tgtaaagatt ttcccagcaa tcatcagact attatttaca gggaatcaaa
     1081 aaaactcctt agtaagctta cacctttagg agttcctgtt tgaacggtat gcataataca
     1141 tttaaatcaa tttaacatta acacctcagt atatggtatc tctacatata cacggtcttt
     1201 ttttggatag tactcgacat ttatattgat agcacttcca ttgtctaacg tagctttagc
     1261 ccggtacgtg ttatcaccat tatcatgagt gatcgtcaca tcatcacatg tccgatttaa
     1321 gttatactgt tcttgtaaaa tctgagttac caatggtaca gatgacttct ctagtccaga
     1381 tgaacccgtc attcttgaaa aaattatacc tataatagcc aatacaacga tacctataat
     1441 gacaaacttc ttattcatat ttactttatt caacttttta tctcctatct ccaaaatgat
     1501 tttctctcat cagtaactca ttgacatccc caatagtaca ccaaagatgg ctccagctac
     1561 ccctgctatc atcaaaatcg tacccatttg ttcatctttc tctcgatata agtaacccat
     1621 aataacagca acagcaccaa ataccacagg aataaatgct aatgatacta ccatagaaat
     1681 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     1741 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     1801 ttcttgtaat gctccgcaaa actggcaata cttactttca tttgttattt ctttaccaca
     1861 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gctaactgtt
     1921 tactttatta aatacatcag aaaacagctc atttattggt acacttaata tctcagaaag
     1981 cattttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     2041 ctgtgtaaca ttcatttttt tggctaactc agtttgggaa agtccttttt cttttctgta
     2101 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     2161 tatgatgtat ttcaactcat aaaatatata aatacttcaa atgtcattac aaatacaaca
     2221 acttgttgta ctctactgag aaggggggtg tattaatgaa acagaagcaa tatgcaaagg
     2281 tcattagaga aaagcgaaaa gcactcgggt ggacacaaaa agaattagct aaaaaaatct
     2341 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagaacct aacctcgata
     2401 gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
     2461 acatagatta tgaggaagag tttttggctc tctatcgctc tcttagtact gaagatgcaa
     2521 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
     2581 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
     2641 aagattaaga gctatggata acctgtctag tttaatataa ttgggtctct tctattcctt
     2701 actctccgtc catcatctct tgttacactt gtaaattgat aaataggaac taattcaact
     2761 cctgataata tcaattcttt tattacttca tctatccctg cccctttcgc tagatatact
     2821 aaaaaactac tgcttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca
     2881 agagtgaatc cttgtttaac aaagtagcct ctgttttcta ttaaagcatt tttcacgctg
     2941 ttatccctga catttcgtag gcgttcacaa acagtgttaa taagttcttt ttctgtcatt
     3001 agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
     3061 tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
     3121 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt
     3181 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
     3241 tctaatcgat agacattgct tctcttatgt ttaaagtttc ctaacgcttt ctcttccttt
     3301 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
     3361 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
     3421 ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
     3481 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
     3541 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
     3601 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
     3661 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
     3721 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
     3781 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
     3841 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
     3901 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
     3961 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
     4021 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
     4081 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
     4141 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
     4201 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
     4261 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
     4321 tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc atctttactc
     4381 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
     4441 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
     4501 gattgtgatg aatttagtat ctctacaaac tcatctcttg taact
//
LOCUS       DADVXO010000161         4478 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.167, whole genome shotgun sequence.
ACCESSION   DADVXO010000161 DADVXO010000000
VERSION     DADVXO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.167"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            151..1032
                     /locus_tag="KB950_002607"
     CDS             151..1032
                     /locus_tag="KB950_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858772.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1045..2655
                     /locus_tag="KB950_002608"
     CDS             1045..2655
                     /locus_tag="KB950_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBB6858773.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2732..3391)
                     /locus_tag="KB950_002609"
     CDS             complement(2732..3391)
                     /locus_tag="KB950_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6858774.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3674..4339)
                     /locus_tag="KB950_002610"
     CDS             complement(3674..4339)
                     /locus_tag="KB950_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBB6858775.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1218 a          862 c          878 g         1520 t
ORIGIN      
        1 ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata
       61 aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta
      121 tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg
      181 aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt
      241 gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt
      301 ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa
      361 gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga
      421 aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct
      481 caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac
      541 tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat
      601 ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa
      661 gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc
      721 agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac actcgatgaa
      781 gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa
      841 gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac
      901 ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa
      961 ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat
     1021 tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt
     1081 tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct
     1141 ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga
     1201 ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg
     1261 aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt
     1321 ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa
     1381 cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct
     1441 gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg
     1501 atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg gctatctgta
     1561 ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt
     1621 tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca
     1681 ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg
     1741 acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta
     1801 atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc
     1861 cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta
     1921 ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg
     1981 gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa
     2041 cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggta
     2101 gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa
     2161 gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat
     2221 tggaccaata tgttactagc tattctattt gctatattgg caatcctgat tagttctggt
     2281 gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg
     2341 tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc
     2401 tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt agcttactcc
     2461 tttgcactca attactttgg cggaattttg gatttaccag aatggttttc caaaacagct
     2521 gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg
     2581 ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac
     2641 ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca
     2701 aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa
     2761 tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt
     2821 ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg
     2881 gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag agagcgactc
     2941 gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct
     3001 caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga
     3061 aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact
     3121 catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta
     3181 aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga
     3241 tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat
     3301 gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa
     3361 caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc
     3421 taataaagaa acataacttc ccttattcca actgataatt ttcttttatc atagcataaa
     3481 cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt
     3541 tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg
     3601 aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct
     3661 tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt
     3721 cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata
     3781 gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg
     3841 tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc
     3901 atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact
     3961 ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat
     4021 agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag
     4081 attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt
     4141 cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa
     4201 tctctttcca tttttgccag tgttctttat attctttctt gatttgttca atcttatctg
     4261 ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat
     4321 ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat
     4381 tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca
     4441 gccaaacaaa aaactgcgat gagcatcacc gcagtttt
//
LOCUS       DADVXO010000162         4377 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.168, whole genome shotgun sequence.
ACCESSION   DADVXO010000162 DADVXO010000000
VERSION     DADVXO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.168"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            192..1493
                     /locus_tag="KB950_002611"
     CDS             192..1493
                     /locus_tag="KB950_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858776.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1520..1780
                     /locus_tag="KB950_002612"
     CDS             1520..1780
                     /locus_tag="KB950_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBB6858777.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2779..4014)
                     /locus_tag="KB950_002613"
     CDS             complement(2779..4014)
                     /locus_tag="KB950_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBB6858778.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1276 a          690 c          893 g         1518 t
ORIGIN      
        1 tttgttaatt atcgaatttt tttgttgctc ctatgattta gaaaacttat tttcaggatt
       61 cttaaaaata aatttgatta tttaagagaa tatattgaaa taaaatttct taattatata
      121 attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt tattgaaaag
      181 gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg
      241 caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt tcaacattgt
      301 cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt cctggatgga
      361 atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg acgatgggat
      421 tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca tatggattag
      481 atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa gttcctgtaa
      541 acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt tctttgggag
      601 catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta tatcgtttct
      661 ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct gttgcccgta
      721 gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg attcgaagcg
      781 tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta accaatattt
      841 tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg ttgtggtcat
      901 ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt
      961 tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta ccctatatcg
     1021 ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca acgatcgtgg
     1081 tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt
     1141 ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct cctatgatgt
     1201 tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact attatcagtt
     1261 attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca tggacattac
     1321 cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt atcttgatca
     1381 tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct tatgataaaa
     1441 agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc taagtttggt
     1501 ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta tctttttatt
     1561 aacggctagt caccttatat tgaatatgct agctacatat catattcaaa tcaatcgatg
     1621 gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg taccgcatat
     1681 gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta tcaattttat
     1741 gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa agtggttgta
     1801 acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt cttaaatttt
     1861 tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg caagcttttg
     1921 accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc cttttttaca
     1981 taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa tggaaatttt
     2041 atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc aaatatagat
     2101 ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg taatatgtat
     2161 gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct gggaatagca
     2221 atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga acattctgac
     2281 atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc atcggattgc
     2341 gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg gtaaatccta
     2401 catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa gcgatgcttc
     2461 tagatctata tgacacattt taagaaaatg tatttgccta attattatgg tctttctttt
     2521 gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac taagaataaa
     2581 tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta aggatgattt
     2641 tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact atttactttt
     2701 tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct ctattaaaaa
     2761 agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga tttcttgaat
     2821 tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat
     2881 cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca ctggttttgt
     2941 gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt gctcaaattg
     3001 catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg tcccttcccc
     3061 gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca gatattctgt
     3121 tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa taggtgcaat
     3181 aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt
     3241 gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct ttcgctgtat
     3301 aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg gaccaacgat
     3361 tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg tgatgagatt
     3421 tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc
     3481 agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt ttgtccagtg
     3541 ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca gcgcatgctc
     3601 tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag gaatcttttt
     3661 attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa
     3721 cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt tggtaaaagg
     3781 gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag
     3841 gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag caaaagtttt
     3901 agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa cctcaatcat
     3961 tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct tcatgaaatc
     4021 ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa aacacagcgt
     4081 tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc acaacttcta
     4141 aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc aacaattttt
     4201 tgatcgattt atatgttgtg ccagataaat acgagaagtt gtgtcattaa aaataaacat
     4261 gagaacataa aaaaataaaa taattatatt aattgatagg tgtgctgtcg tcattataca
     4321 cacgtaagaa agaaatagtt atgtttaaaa atattcttag actttctcgc catgaca
//
LOCUS       DADVXO010000163         4341 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.169, whole genome shotgun sequence.
ACCESSION   DADVXO010000163 DADVXO010000000
VERSION     DADVXO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.169"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(94..579)
                     /locus_tag="KB950_002614"
     CDS             complement(94..579)
                     /locus_tag="KB950_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBB6858779.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(581..1345)
                     /locus_tag="KB950_002615"
                     /pseudo
     CDS             complement(581..1345)
                     /locus_tag="KB950_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1515..2708)
                     /locus_tag="KB950_002616"
     CDS             complement(1515..2708)
                     /locus_tag="KB950_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6858780.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2792..2992)
                     /locus_tag="KB950_002617"
     CDS             complement(2792..2992)
                     /locus_tag="KB950_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB6858781.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3416..3649)
                     /locus_tag="KB950_002618"
     CDS             complement(3416..3649)
                     /locus_tag="KB950_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858782.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3656..4087)
                     /locus_tag="KB950_002619"
     CDS             complement(3656..4087)
                     /locus_tag="KB950_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6858783.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1089 a         1072 c          839 g         1341 t
ORIGIN      
        1 attaactgat aaaaaaatat ctactattta aagatacagg gataagtaag taaaaaaata
       61 acatgtcatt agatagtaga cagttccaac tctttattta ccttttttgt atgctgcact
      121 gtcaatccaa gttcctaaat tgttgtatcc atcatcactt atataatcat attggccatc
      181 aataatttcg tcaaaccaca aatattctcc atctggtttt tgcccaatta ttttaattac
      241 tgattgacta gtagttgtca tctggttaaa atcttttatt ttacctatat taacggccaa
      301 aatagcattt cctcgcttat aactctcctg gatttcatat ttaacatatt ctcgatcaag
      361 agtttcttta cctatcagca ccacagtcac tgatgttcca attaattgat tatctatcca
      421 gttttttata gcaatatctc cattacgttt aactttctca aattcagcct tatctacaaa
      481 accagcttcc tttgttcctc gcaaagtcca gctgtttcta actaccattg ctcttgttac
      541 atctgcataa tggaaactaa aaaaaaccct tctcaccata ttaatcctcc tgaatactat
      601 ttattaaact gacaatttgt tcaacttttt tatctacttc tccttcagta tctaattttt
      661 ctagcgcttg aaatttactt tttaaggaat catcaaaata atcactgaaa ttctcttgaa
      721 tcttatcaaa caaaacctta cttgcacctt tggtaaatcc aatcggaact actatatttt
      781 cattttgaac agataattca aattcttcaa tcattccatc tgctgtaact atttcacctg
      841 attcaagttt gtttccaaaa acaaaaatac taatcccaga ctctgcaata atgttttctc
      901 gatatcttct ccactcatga ggcatatcaa ggggaagtgg tcttaaagaa agtacgtctt
      961 gtatgttatt ttttcttaaa tttcttctct gctttagaac tcctgaaaca atactattac
     1021 caattccaag tccgtaacct gatactattc tataaccgga ttcagaaagc ctatatgcta
     1081 atttctcgac aaattttaat cctttcgatt cagatgtaaa tggagtataa tcaacagcac
     1141 ttcccgaaat aaaaatagtt tttctcaaat acttttttct aatacttttc aaaatatcag
     1201 taatctcaga ataatcatct acttcaacaa cgtttatcga aaatctggac aaatcattga
     1261 tttgtagtct tctttttgtc agttcataat tatatctctc ttgatcaaaa aaatcctcag
     1321 aatcgacaaa attttccttt aaaattatat gggattttag attatctttt tagaagttct
     1381 tgaatgtcgg tatagcccgt gtttccgggc ttttccggca tttttgatat tgttagatac
     1441 ttacaaatgt tcttattacc tctcaccatt tccccctcaa aagtagtaaa tggagtagta
     1501 aaaccggcct tgtgtcaggc tgccagccgt tccatttctg cccttgcgga atcgaatgtg
     1561 gcgtgtgcat aatagttcag cgtcatggtg atattggaat gtcccataat gtactgcaag
     1621 gctttggggt tcattcccgc attggctaac cgggtgcaga atgtatgacg catagcatgg
     1681 ggcgtcgtcg ttttcggcaa cgcttcttca tggctcttgt tgtacttctt cacaagcctg
     1741 ccaaacatcc cgccataatc taccgccgtc atgggatagc cgttctgttt gagaaacagg
     1801 aaattggcat acccgtcaat aatgaacggc tttgcacctt tgcggttttt tatcactcgc
     1861 tggaacgcct gatacacttc ctcattcatg gggatttttc tgacgccgct ttgggtcttg
     1921 ggttcgtcaa tatagtaacc gacgccggta tttctcaaaa gctggtggct cacgatgatt
     1981 acccggtttt caaaatccaa atctttgtcc gtcagtccgc acagttccga aatgcgaagc
     2041 cctgtcccca gcagaataac gattgcgtca taatgctttt ggtaaacctt atcaccttga
     2101 ataaaggaca gaaaactttc ttcctgtgct ggtgtaagag gcacctttgg ttccgtatcg
     2161 tcgtccagaa cgtcactaag ctggaagtca aacgggtttt ttcggatgca atcgtcctgt
     2221 atggcggtgt aaaaagccgc tttcagggaa cgcttgtcgt tattgatggt cttataagaa
     2281 aggccctttt ctttcatccg aagcgcccat tcctttgcgt cggacagctt cacactgtca
     2341 atcgggcagc tccccagccg atcttgttcc agaatacgca tcagccgttc ccggcccagt
     2401 ttggtgttgt gcttcacatt gccccggtgc ctgatctgct tggcgtagag ctggcagacg
     2461 gtcattttct tgccgatggt gtctatcccg tcgtcaaggt ctttctggat ttctttctcc
     2521 ttttcccgga gtgaaatatc ctcccgttta cctgccgggg tcttatccgt aggcactaac
     2581 ttccatgagt aaacaaactg cggttttcca aaggtatcaa catatttgta agcatatcgt
     2641 ccgtcttttc tctggctctc tccagaccgc aaaacgcgat ttttgctgtc tcgtcttttt
     2701 tctgacatct cgtcatgctc ctttccatga cggaaagagc cgcatatatg cttataaatt
     2761 attataacat atctgcggcc ctttttcact attagattgt gtccaaagtg tcaattattt
     2821 tctcaaactg cttgcgtttg atctggatgc ggttgccatt cataatgacc cagccagagg
     2881 ccggattttc ctcggccagc ttacgcaatt tgttttctcc gatacggaaa tatttagacg
     2941 cttcctcaat cgtcagcgtg tatttttccc aaataggcac atcatggttc ataatgcagc
     3001 cccctttcac aaattcaaag gtttgtgtga tttaggggca tagacggacg gctgctggca
     3061 cagctccacg ggactttcac ccccgcgtgg ttctcacgcg gccctaccca ttgcctgcga
     3121 cgcttttaac gctcggactg tggctataag ggagtatcat agtcctgcct gtacgtcgtc
     3181 gcccgcaagc cgccacgctc acattgcggc gtggtgttcc tcgctccgct tttcccgtag
     3241 ggaaaagtat catggcgcac cagccgtccg gctcggcaca gacagaatgt gtccgtatgc
     3301 cctatgatgc gccgccgtta tcttgcgggc atatttatca aggtgcgatc aggccaccgg
     3361 gaaaacaccg aaacggggaa agggtaagcc gtttcatgct gccggggccg gttcgtcagt
     3421 tcgtgacgat agcgtggatg agcttgattt ccagacggcg cttcatggcc tcgtccagac
     3481 acacatgcgg atagccgttt tcgtcataga gcgtccgggt acacagcttg ttcatgtaac
     3541 cgctgtaata ctgcaaaacg cgctctacgg ctccggcatc cccagcgcgg gcggcctcga
     3601 tcaccgaaag gggcaaaagc tcaatgccct tgtagttcgg gttcttcatc cgcgtctaac
     3661 ctcccttcgg catcaacgcc tttagctgtt tccgcagctc cgataaggta tttgtcctgt
     3721 gccgctgaac ggcactccgg gacattccca caaggccgcc gatctcacca tctgccatat
     3781 ccagaacaca gtgcaggatt aaaatactct gctccatgct gggcagcgcg gcaaaggctt
     3841 cggcaacaag ctcattgttg atatgtaggt catagccatg cgacgaaaac gtaaagctgt
     3901 cgctggggta gtgatctact gtacagagct tgtccaaatc tgcctgtgac aaagagctta
     3961 gtgacacttc acggtcacgg cggcgcttca tttcagccaa gtagcttttg gcctcgttac
     4021 gcagtaccgt cttgcaaaaa gcatcgaaca tgcaccgcgc ttcatagtcg cgggggatag
     4081 catccatctt ctcaccccct ttctgtgggg gacttaggtg gttctctccc tttccgctaa
     4141 caagactttg acaaggccgc cagctacccg ctaaatacct cctttttgaa aatttctcaa
     4201 aaaatttttt tggggacagc cgggccgcaa aagacgacaa aaatcgcccg gcaggtcgca
     4261 aacctaccgg gcgagaaaat gctttgctat gctgctgatt tacatggtat agtacatact
     4321 cataaccgga atagccctgt g
//
LOCUS       DADVXO010000164         4183 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.170, whole genome shotgun sequence.
ACCESSION   DADVXO010000164 DADVXO010000000
VERSION     DADVXO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.170"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..390
                     /locus_tag="KB950_002620"
     CDS             <1..390
                     /locus_tag="KB950_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6858784.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            454..921
                     /locus_tag="KB950_002621"
     CDS             454..921
                     /locus_tag="KB950_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858785.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            1126..2790
                     /gene="alsS"
                     /locus_tag="KB950_002622"
     CDS             1126..2790
                     /gene="alsS"
                     /locus_tag="KB950_002622"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBB6858786.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2803..3513
                     /gene="budA"
                     /locus_tag="KB950_002623"
     CDS             2803..3513
                     /gene="budA"
                     /locus_tag="KB950_002623"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBB6858787.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3643..4140
                     /locus_tag="KB950_002624"
     CDS             3643..4140
                     /locus_tag="KB950_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBB6858788.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1404 a          677 c          875 g         1227 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccgttgcatc gtgatgatag gtatgacaga agaattagca
      481 aaagaaaaaa gtttttcacc agtacgtttg ttcataaaga cgtttcgcca attttggcta
      541 aagggatttt tttattggct atttgcttgg atagttagtg tgataatgat ttttgattgt
      601 ttctttttta tacgtttttc ttatggaaaa tggttgattc cgttgtttgt tttacttgct
      661 tgtttatcag tttcctttag catcaactac tggtattttc aggtgagaaa tccagcgtcg
      721 aaaccaaatc aagttctacg gatagcgttc tactatacgt tgaaaaaatg gtatgtgagt
      781 ttactagatt ttttactatt gacaagtttg tttctcttct ttttcgtaaa gccccaatgg
      841 tgtattctac taggtccttc tatcgtattt ggtttgattt acttcaacaa tcgtaaattg
      901 atgagaacta tggatctgta gactttataa attgaaactt tcaattaaga atgacagttt
      961 ttatgtaaaa actgtcattc tttttcattt tttgaaacat atggctaaac aaatgaaata
     1021 aaacgtgcta tacttttgaa tgaaacaggt ttagaaactg tatcaattat ttgtgattat
     1081 ttctaactaa taattaaaaa taaagcataa aaaggacgat gttcaatgga cgaaaaaatg
     1141 aaacaaggtt cgaaatctgt agtagaaagt ttgattaatc atcatgttga ttatgtattc
     1201 ggtataccag gtgcaaaaat cgatggtgta tttaatgaat tagaggatca aggtcctgaa
     1261 ttaatcgtga ctcgtcacga acaaaatgct gcatttatgg cacaagcaat cggacgtatt
     1321 acaggagagc caggagtagt tatcgcaaca agtggtcctg gtgctagtaa cttagctaca
     1381 ggtttggtaa cagcgacagc agaaggtgac ccagttttag caattgccgg acaagtaaaa
     1441 cgaagcgatc tattgaagtt gacccatcaa agcatggata atgcagcact gttccagcca
     1501 atcacaaaat acagtgcaga aatccaagat ccagaaacaa tttcagaagt gattgccaat
     1561 gcttatcgta tggctaagag ttcaaaaaaa ggcgcgagct ttatcagtat cccgcaagat
     1621 gtagtggatg cacctgtcca agggaatgtc atcaagccat tgagcgatcc taaattaggt
     1681 tctgcttctg cagatgatat ccgttaccta gctgaacgca ttagagaagc aaaactaccg
     1741 gtactattag taggtatgcg aggatcaagt gaaaaagaaa cgttagccat caggcaatta
     1801 gttggaaaga cagcattgcc tgttgtagag acattccaag cagctggagt gatctcacgt
     1861 gaattagaag cgcatttctt tggtcgtgtg ggtttattta gaaaccaacc aggagatatg
     1921 cttttgaaac gaagtgatct agttattgct attggctatg atccaatcga atatgaagca
     1981 agaaactgga atgcggaaaa agatgcgcgg atcattgtga tcgatgaagc gccagctgaa
     2041 atcgatcctt ttatgcagcc tgaaagagaa ttgatcggtg atatctcagc cactcttgat
     2101 ttattgacag gttcattaga gcctcaacaa gtgtcagaag atgcaaaaga atatttagca
     2161 tctcttcaag cagaattaac ggagcgggac attgttcaat caaaaggaga agcaggtatc
     2221 ttgcatcctt tagaagttat taacacgctt cagtcaaaag taacagatga tatgactgtt
     2281 accgtagatg taggtagtca ttatatttgg atggcacgcc actttagaag ttatgaacct
     2341 cgtcatttgt tattcagtaa tggtatgcaa acgcttggtg ttgcattgcc atgggcgatt
     2401 tctgctgcat tggtgcgtcc aaatacacaa atcgtatcag tttccggcga tgggggattt
     2461 ttattctctg cccaggattt agaaacggcg gtacgtaaaa aattgaatat cgttcatttg
     2521 atttggaatg atggacacta caatatggta gagttccaag aaaaaatgaa atatcagcgg
     2581 gcgtcaggtg tagactttgg tccagttgat tttgtgaaat atgcagaagc ttttggcgct
     2641 aaaggaatcc gagctacaag tgtagaggaa ttagaaaaag ctttagaaga aggatttgcg
     2701 acagaaggac ccgtaattat tgatattccg attgattacc gtgataatga gaaattggga
     2761 gaaacaatct taccagatca attctattaa ggaagtgtcg acatgactga aaaaatctta
     2821 taccagcacg ggacattagg agctttgatg gctggcttga tggatggaac agaaacgatt
     2881 gctcatatat tagaaaaagg aactctggga ttgggaacat tgcatgggct tgatggtgaa
     2941 gtcatctttt tagatggcgt agcttatcaa ggaagatcag atggctcagt tgcccaatta
     3001 gatggaagtg aacttacccc ctatgcagca attacagatt ttacgccaga tactagtttt
     3061 tctgtctcaa aaactgcaga tggagaacaa ttgaaaaaag acatattagt aagagaagcc
     3121 ggggaaaacc tatttttggc agtgaagata actggattat tcaaaaatat gcacatccgt
     3181 atcatgccga agcaagaaaa accttacaga agactagcaa aaatttcaga gtctcaacca
     3241 gaattccaac aaagcaatgt ccaaggaaca ttagtcgggt tctttacgcc tgaattattc
     3301 caaggagttg ctgcagcggg atttcacttg cattttatcg atgatacaca tacatttggc
     3361 ggacatgtaa tggattttga agtggctgaa ggaaaagttg agattagccg catctcgtcc
     3421 cttgtacagc attttccagt tgatgatccg acttttgttc acgaaacgat tgattatgct
     3481 gatttagcag cagaaatcca acaagcagaa tagaaaaaga cagcgagatg cgttcaaggt
     3541 gattaacctg acgtatctcg tttttttgag gaggaaacct agcgtttaca aaaaaactga
     3601 gctataatct tctccataga tcaaaaagga gcggttttta tcatgtggac agaaaaacag
     3661 aaaaaagatg catacgaaat tttattatta cagcaacaag gattattaga agcagaagat
     3721 aactggttag caaatttagc aaattcatct gcattgttga atgagacttt gcctgagact
     3781 gtctttgcag gatattattt attcgatggt aatgaattga ttttaggacc gttccaaggg
     3841 cgagtttctt gcacacgtat caagatggga aaaggagttt gtggagaatc agctgaaaaa
     3901 caagaaacat tgatcgttga tgatgtcaaa acccatgcaa attacatttc ctgcgattca
     3961 gccgcacaat cagaaatcgt agtgccaatg gtaaaagaag gtaatttaat cggtgtttta
     4021 gatatcgatt gcggagtaac gaaagggtat gatgaaatcg atcaagcatt tctagaaaaa
     4081 tatgtagatc ttttattagc aaattttaat ataaatgctt tttcatttat taataactaa
     4141 aatcgtctat tgaaacaaaa aagaagagaa atcaagaata aaa
//
LOCUS       DADVXO010000165         4146 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.171, whole genome shotgun sequence.
ACCESSION   DADVXO010000165 DADVXO010000000
VERSION     DADVXO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.171"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            107..562
                     /locus_tag="KB950_002625"
                     /pseudo
     CDS             107..562
                     /locus_tag="KB950_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            659..2908
                     /locus_tag="KB950_002626"
     CDS             659..2908
                     /locus_tag="KB950_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6858789.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            2911..4086
                     /locus_tag="KB950_002627"
     CDS             2911..4086
                     /locus_tag="KB950_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6858790.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1456 a          596 c          756 g         1338 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatc aggacaatct
      121 agtcagtgaa ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc
      181 agctagttat ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac
      241 aattgaaata atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag
      301 tctatcgata cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat
      361 aaaagagtat attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga
      421 atattcaatc cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa
      481 agcctttaag aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct
      541 aggaatcatt aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa
      601 acgcttacta tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat
      661 gtataaggat aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt
      721 taatcaggag ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata
      781 ttatggtgag aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc
      841 tatgacaacc tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga
      901 atttcctgaa tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa
      961 tggctctcgc ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa
     1021 actagaaggt ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt
     1081 ttatatttta gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga
     1141 ttatcctgtt attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat
     1201 cgagaatatt agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt
     1261 atctggtgct tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca
     1321 atcaatcgaa tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa
     1381 aagaaaaaat actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg
     1441 aaacttctta attcaagcag aagtagatac atgggatgtt actagactac aagttggcat
     1501 caatccattt ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc
     1561 gattctggtt tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt
     1621 tagaaaacgc ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa
     1681 ttgggaagca acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc
     1741 atttgatgtt ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa
     1801 cgattgtgca ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg
     1861 cagcctttca gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga
     1921 aatggtgaat aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc
     1981 taatcgaaaa cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt
     2041 tgttgaaaat attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat
     2101 taagtgggat atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca
     2161 acaaggagaa gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat
     2221 aacaaaatat cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc
     2281 tggaatgctt cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag
     2341 gttaaaaatt caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt
     2401 atcaattgtt ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt
     2461 agcttttttt ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt
     2521 gaggattgta aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg
     2581 tactttttat agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt
     2641 tagtcatgat aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc
     2701 tccttaccgc cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca
     2761 aaatagtttg cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc
     2821 tagtggacaa ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat
     2881 ttgggttata acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa
     2941 ggagcaatct taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt
     3001 ccacaaatga aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca
     3061 ccaagtataa ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga
     3121 atgaaaaaga caataatttt aggtttatta ttagctggta taggaggaat tattccagta
     3181 tttacagcta ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga
     3241 gtatataatt cattggctgt gacatatatc agctctttat actctggcaa tactagagca
     3301 acgctattag gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt
     3361 gctggagttt tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt
     3421 cctgtagctc tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata
     3481 cctgaaaatc atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca
     3541 atcttactcg taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa
     3601 attaaagggg taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata
     3661 ctagcaggat ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg
     3721 ggaattatat tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg
     3781 ttcctgctaa cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc
     3841 tttagtaact tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt
     3901 tttattggct gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt
     3961 ctaacaagaa gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg
     4021 atcctatttg taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct
     4081 agttaatttt aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga
     4141 cttaat
//
LOCUS       DADVXO010000166         4088 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.172, whole genome shotgun sequence.
ACCESSION   DADVXO010000166 DADVXO010000000
VERSION     DADVXO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.172"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(24..1790)
                     /locus_tag="KB950_002628"
     CDS             complement(24..1790)
                     /locus_tag="KB950_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858791.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1750..3504)
                     /locus_tag="KB950_002629"
     CDS             complement(1750..3504)
                     /locus_tag="KB950_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858792.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3614..>4088)
                     /locus_tag="KB950_002630"
     CDS             complement(3614..>4088)
                     /locus_tag="KB950_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBB6858793.1"
                     /translation="FGHSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLFFTL
                     MGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTD
                     RFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1370 a          780 c          581 g         1357 t
ORIGIN      
        1 aggttaattt aaagcctatt tcatcatcgt gattgagctt ctttttcata gacgaatttc
       61 tcagatagtt tagttttctt tatgagattt ttgtatattt ctgaccttcg atacaactca
      121 ttatgagtac cttgatcaac tagttttcct tgatccatca ctacaatttt atttaccttt
      181 tcaatagact tcaagcgatg cgatataatt agtactgtct tatcttgtat taaacgattt
      241 aaactttcct gaatacgttt ttcattttcc gcatctaaac tggcagtaat ttcatctaaa
      301 attaaaattg gcgcattttt caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct
      361 ccagaaagtg aacttccgtt ttctccgacc aatgtatgat aaccgtcagg tagtttttca
      421 ataaagtctt cgcagttagc taaacgagct gcttttttca cttcctcatc tgacgcttga
      481 ctatttccaa tacgtatatt ttccaaaata cttgtattaa acaaagttac ctcttgaaaa
      541 acaatagcga ttttgctaaa caaagaagct ggagatacac gtttaatatc atacccatca
      601 attaaaatac agccttcatc ataatcatac agtctagaca ctaatttcaa gatactagtt
      661 ttcccggaac cactagcacc tactaacgca gtcacttctc cttgtttagc cgtaaaagag
      721 atatgatcta aaattggggt attgttatca tatgagaaag agacatctcg caactcaatg
      781 tcaaaagatt tcagaggact atctgatccc tcttggatac tggtttcctt catagctcta
      841 attcgctgta ttttaggagc taaataaaat atttcaagca ctgcttcttt catagaatcg
      901 aaggaatcct ttatttttat tgcggctagt aagtaaccaa cgacgtagag tatagtcact
      961 tcacccgtta acagtaagtg aacacctact agaatgacta ctgctaaact aacatatgag
     1021 aaaatagagg ataaggccat gactgaaaaa gtacttaatt ctacctttaa atgaatcctt
     1081 tcactctctt ccattttttt gaaaagtctg tcttgaactt ttttagatag attaaagcta
     1141 ttaatctctt gctgcaattc tatagtttct tgaaattctt ccgagttctc tctcaaagta
     1201 tcgtaatatt tagtattggc tttcgtttgg gatttctttg ataacaagat taagacaaaa
     1261 ctaaataacg ttggcaaaat aacagctaat cccattttga cattaccaac caaaagcatc
     1321 actgaaataa aagggaaaaa cagagccata ccgccggctt taggtattgc atgactcatc
     1381 gcatgctcaa taccttcaac gtcagacatg atagtttgag ataaatctga taaattatgt
     1441 tttgaaaaat aggataatgg taaatttgac aaatcgtctg caatttgcac tcttaaatgc
     1501 gcactttctt tataagtact attatataag ttttcatact ccctatctaa caaccaatac
     1561 atgaaaagca aagtaacaaa cgatacggca aaataaagcc aacggggctt tgtattttct
     1621 aaaactaatt cgtctatcag catcattata atgaatgctg gtaccatatt aatgcagtat
     1681 acaaaaaaac tagaaataga agctttagtt aaagcttctg cgccctgatc acttaaagca
     1741 aatttgtttt tataaaaact tttcattgtc aagcctccaa tcatttgcat tctcataatt
     1801 agtgaccaat tgtttataca cttgatgttt agccattaat tcattatgat ttccacgttc
     1861 tactattttc ccttctgaca agacaagaat ttcatctaca gatttaatgc tggataatcg
     1921 atgagctatc ataataactg ttttttcttt catcagatgt tgaaaagcct tttgtagttc
     1981 atactcatta tctggatcaa tagatgcact ggcttcatcc ataataacaa tctttgcatc
     2041 ttttaaaatt gctctggcaa tagctatacg ttgtttttct cctcctgaca gataaactcc
     2101 tttactacca attaaggtat gctctctttc gggaaatttt tctaaaatcg attcgcaacc
     2161 ggctaatctt agcgcgtcaa atactttact tgctggcgct tgaggatttg ctatagaaac
     2221 gttatcgtaa atactctgat tgaatagttt cgtatcttga aagacaaaag caattgtgtt
     2281 aataattgtg tcttttgaat agtgatttaa aggcttacca ccaattttaa tctgtccctt
     2341 atccacattg tagaaacctg aaattagttt agctatcgta ctctttccgc ttcctgattt
     2401 tcctactaat gcataaattc ttttttgttc taaaactaag ttgaaatttt ctaaaacaca
     2461 ctcttctttg tatgaaaagc tgacattttc aaatgcaatg tcacaatttt taatctgtgt
     2521 ctcgttccca tattttagtt ggtcttgctt catttctaaa taaagggagt ctagtgtttc
     2581 gactgcatat cttgcttcaa acaagtgcat acttacatac attattctca tgaaagaaac
     2641 aaaaataatt ccacttaaaa aaaacaacat caacaagtca acagctaata tcttgggagt
     2701 ggtaagagaa gttataaaaa aggttatagg aactatcaaa atgcaaacta tgccaaaaaa
     2761 tagccattga aaaagaacaa aaggtgtttt cccactcaaa gaatacttat atgcaaactt
     2821 agcataatcg ttaattgctt tattcaaggc tttaaatgat gttactttag caccaaatat
     2881 ctttataact tgaattccgc ggatatattc taccgtttca gagcttaaca catctaacga
     2941 ttcttgatat ttttgaataa aatttgtatt ctctgtcatt ttctttaaca agaatacacc
     3001 aattactaac atggcgatta gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc
     3061 aactgataaa acaggtgtta ctatggcttg agcactatct ggaatcaagt gagcaattgc
     3121 catatgtgtt ttagccgcgt tgtcatcaat tgtctttcga atatatcccg aagaatttaa
     3181 atcaaaaaaa cgaaaacttg cttctgttaa accgctaatt cccttttttc tcaaattagt
     3241 ctctaagcga aacccaagga tatgagaaaa taagccagaa aaaatataaa ataaagctcc
     3301 taaagttaac caagcaacag tttgaaccgc ataactctta gctaaatatt catctcctac
     3361 aataattaga gactgaagaa atttgtaaat actgtaatat ccatagactg ttaatacgac
     3421 agaaaaatca gaaaataaag tggctaacca tccgtagtat ttcattttag ggacataact
     3481 aaataatttt ttgtatactt tcattttttc ctcccccgca ttcaatttct tctcttattc
     3541 gtttacaact gttaatgctt tccgtaatag cgtaaattag agatgtacaa acagataaaa
     3601 ggtaaactac gttctatttt ttgtattttt ttaagacttt tcttccaatc aatacaccaa
     3661 tcatagataa acttgctaca actaacactg aaatgattcc ccatatacct accgcatagt
     3721 ggatcattgc atccattgtt gcagcagatt gtcctttatc tatccaatct tgacgaaaac
     3781 gatctgtaaa gaaccatata ggaatccact gtccggcagc actgcctaat tgcattagag
     3841 cccaagacgc aacttgagga attgctcgat catatccaaa tacagataga ataatatcag
     3901 ctaagatcgc catactaatc gaactgataa accaaggtaa gtacgccccg cccattaacg
     3961 taaaaaataa caataaaaca attgtgtaaa tagtaaaaat tccttttcta gggtagttat
     4021 agctaatata aacaaatatg attccagata ataatccaaa aatcccgggg gatatgctgt
     4081 gaccaaac
//
LOCUS       DADVXO010000167         4067 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.173, whole genome shotgun sequence.
ACCESSION   DADVXO010000167 DADVXO010000000
VERSION     DADVXO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.173"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            86..1435
                     /locus_tag="KB950_002631"
     CDS             86..1435
                     /locus_tag="KB950_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6858794.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="KB950_002632"
     CDS             complement(1563..2153)
                     /locus_tag="KB950_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBB6858795.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="KB950_002633"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="KB950_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBB6858796.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1173 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DADVXO010000168         4044 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.174, whole genome shotgun sequence.
ACCESSION   DADVXO010000168 DADVXO010000000
VERSION     DADVXO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.174"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(153..1658)
                     /locus_tag="KB950_002634"
     CDS             complement(153..1658)
                     /locus_tag="KB950_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6858797.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="KB950_002635"
     CDS             complement(1655..2341)
                     /locus_tag="KB950_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6858798.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="KB950_002636"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="KB950_002636"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBB6858799.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1114 a          856 c          692 g         1382 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa agggatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat attt
//
LOCUS       DADVXO010000169         3904 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.175, whole genome shotgun sequence.
ACCESSION   DADVXO010000169 DADVXO010000000
VERSION     DADVXO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.175"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            356..1849
                     /locus_tag="KB950_002637"
     CDS             356..1849
                     /locus_tag="KB950_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBB6858800.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            1984..2280
                     /locus_tag="KB950_002638"
     CDS             1984..2280
                     /locus_tag="KB950_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBB6858801.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2381..3232
                     /locus_tag="KB950_002639"
     CDS             2381..3232
                     /locus_tag="KB950_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858802.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3253..3536
                     /locus_tag="KB950_002640"
                     /pseudo
     CDS             3253..3536
                     /locus_tag="KB950_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1385 a          568 c          799 g         1152 t
ORIGIN      
        1 tacaaagtag caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac
       61 ggaaataatt gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt
      121 cacgctcgtc tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga
      181 taccttttcc gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt
      241 ttatttagtg tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc
      301 tgagattaaa gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt
      361 gagcgaaatt tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag
      421 caccatgaaa cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg
      481 tagcaagcaa ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt
      541 agagaacgaa aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc
      601 ggatgattat cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac
      661 atttgtagtt gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt
      721 agatcaagtg ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta
      781 ttttgtacta aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt
      841 agcaaaaaaa atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga
      901 tctaggatgt aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa
      961 agaaatgaac tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc
     1021 agatcaaaaa acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga
     1081 aattcgtcaa gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg
     1141 cggagaagga cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc
     1201 ttcaggttat gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca
     1261 ggaaccgcta tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta
     1321 tcaagcagca agcagagaat tcattattga attgtgccag caatgggttt caccaacctt
     1381 aaaaaataat gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga
     1441 tcaaagaaaa tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga
     1501 aaaaagtctt gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact
     1561 aacgatgccc aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt
     1621 cttctacaag gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt
     1681 gttcgcgagg gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac
     1741 agaggtattt ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc
     1801 cttgaaccct cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg
     1861 tgcaaattgc cttatacaat atatctatga tacttctgta ggtgttgctc cctttgccta
     1921 gctacgtgta ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt
     1981 tgtatgtcgt taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt
     2041 ttgggattat ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct
     2101 tcttgggtta ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg
     2161 tccttagctt ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag
     2221 gagtatattg agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag
     2281 tagtttttat ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa
     2341 aggaaaacgt taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat
     2401 tgaacacacc tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca
     2461 attatggcca ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt
     2521 ttactcgatt ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc
     2581 agcatcagac atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt
     2641 tactcaatta gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag
     2701 cctattcgat ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca
     2761 atctggaaga acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa
     2821 tggacacatg tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac
     2881 gaaacttatt aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct
     2941 tggttatgag tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt
     3001 tcacacgagt atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca
     3061 ggaaatgaaa gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag
     3121 cctacgatat ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc
     3181 atacattttt tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag
     3241 aatcgtttga aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa
     3301 accttaaaga aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt
     3361 aaaacgggta cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact
     3421 gattttttgt tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc
     3481 aattgattga tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc
     3541 ctttatttgt aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata
     3601 ggctgtgcct attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat
     3661 agtgaaagga tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct
     3721 ccttttcgct atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg
     3781 gatgagaaaa tcgtaagaga aattgatcga agatgtgacg aacggttctg ttgcaaagtt
     3841 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
     3901 aatc
//
LOCUS       DADVXO010000170         3876 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.176, whole genome shotgun sequence.
ACCESSION   DADVXO010000170 DADVXO010000000
VERSION     DADVXO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.176"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(716..3865)
                     /locus_tag="KB950_002641"
     CDS             complement(716..3865)
                     /locus_tag="KB950_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBB6858803.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          635 g         1362 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc ttccct
//
LOCUS       DADVXO010000171         3858 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.177, whole genome shotgun sequence.
ACCESSION   DADVXO010000171 DADVXO010000000
VERSION     DADVXO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.177"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..892)
                     /locus_tag="KB950_002642"
     CDS             complement(<1..892)
                     /locus_tag="KB950_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB6858804.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
     gene            complement(1349..1777)
                     /locus_tag="KB950_002643"
     CDS             complement(1349..1777)
                     /locus_tag="KB950_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858805.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1805..2443)
                     /locus_tag="KB950_002644"
     CDS             complement(1805..2443)
                     /locus_tag="KB950_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858806.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2447..3217)
                     /locus_tag="KB950_002645"
     CDS             complement(2447..3217)
                     /locus_tag="KB950_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBB6858807.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1181 a          730 c          570 g         1377 t
ORIGIN      
        1 tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg atttttacat
       61 tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca cttaattttg
      121 tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg cctttgtttt
      181 ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca ttggtagaag
      241 tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg acttctgccg
      301 gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt gtcgtagtat
      361 cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca gctttcttca
      421 catcttttgc atcatcttca cttgctactt ggataatttg agcttccact tctggatgga
      481 aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc tttagacctt
      541 cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg taacctgcag
      601 atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca tattgagaat
      661 cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga taaacgatca
      721 actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt acttcttgtt
      781 cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc gtacagcctg
      841 acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc atttatcaat
      901 tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga tgaaaatcat
      961 caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc tacacaatcc
     1021 agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat atactacttc
     1081 ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact aattcttaga
     1141 acaaatgtta tcgattttgt ttttcttctt ccgctgctgg gatattcaaa tcagacactt
     1201 ttatgttcga ttttccagac atttttttgt tgtttttatt atgatatatt tttatacaac
     1261 taaaaatgaa tatattaaaa ctaccaaaaa taacttttaa cgctgtttca ttatatgttt
     1321 tttctatttt atataaactt ttggatactt aataccaacc ggttggattg taccaacatg
     1381 agaatgtgtc agttctattt cctgtcgcac ttgcataggc ccatactcct tttgttgcat
     1441 ttgctgatcg aatagagttt ccaactttcg cttttgcacc gtgacgatta tagacacgac
     1501 tatagtggtt agaatgacct gtttttctcg tacaagttgt atcactataa taaacccatc
     1561 ttccagagct atgattaatt gctgttctag aaatttcccg gtctactggt aaatttgact
     1621 taataatatt acctttagca tcaaaatgaa tctctcctgg ctttaaagat ttgtaataga
     1681 tttcaccgtg aactgaattt gcttccactt cagatgacat agcgtttcct ccaacaaaac
     1741 ctatccctag tgcacaaata ataactgctt ttttcataaa acgacatcct ttcctttttg
     1801 atttttaaag catgatcttc ttgtttccta ttttggtcag atatggatca tgcgtgacta
     1861 cgataactaa cgtaccttgg gaagcttgtt gcgataagat ttcaactact ttatccttat
     1921 ttcttttatc caatgaccca gtcggttcat cggctaaaat gattttgcta ttcttcaata
     1981 agactctcgc acaggcgatt ctttgcttct caccaccaga taatgtgtag ataggcgtac
     2041 ttagcttctt gtttatattt acttttttca atgcctcata ctttgctgtt cgcttatctg
     2101 aaacatttaa attcgaaaac ttcaatccaa tatctaaatt aaaatcgatt gtttcatatt
     2161 caatcaaccc aaaatcttga aaaagaaaag atatggtatt tctttttatc aagcgcttct
     2221 ctctcccagt taattgtttc gtctcttgat cgaacaaata gaattgtcct gaactgaact
     2281 gatcattcaa tcctaatata ttcaataacg tacttttacc tgctccactt tctccaacaa
     2341 cagtcacaag atccccagca tccgcagtga aacgatccaa atgaagaatc gttttgtttc
     2401 gaatgatttt tttcgcacct tttatcacta tcaacggctc caattgttaa cctccattca
     2461 tcagcaataa ctgacttttc ttttctgtag acaaaataaa aacaactgaa agaataaaat
     2521 caatgaagct aaagaaaaaa atattaaaaa gatatttagg ctctctttct ataagaaaaa
     2581 ggaagattac acttgtcgtt ccccatttga tgaggttcat catgaagaaa tctttatacg
     2641 tttcaaagag cgaataacca cttgaacgaa agatagcgat tttccgacaa ttgtagacaa
     2701 aatatatgca taacatgaca tagctcaaca agaagaaaga aaagaaagtg agtaaaaata
     2761 tctgtatcaa gtaatgaaat gatctgctta acactttgac agctatctct tcagtctgag
     2821 tatacggaaa tgataactgc aagttatcac gtaactgact ttcttcaatc agctcaaata
     2881 acctttcttt cgacaaagag cctatcttga tctttaacgg tggatagatt tctccgctga
     2941 gaatatttct ttccaaatac atactgtttt tcaatgtcag tatttcaaca accggataat
     3001 gttcgatcca ggggatagaa ggaataaatg tataaatggg ttgattgttt tttataaata
     3061 gaaatttcgg ttctttttca ctgatttctt gataatattc tgttatttgc gccaggtcat
     3121 tttttaaatc tgtatgtcta tatcgttcag ggatcaatat aacagggtgc gtttcttttt
     3181 cggttatttg gatcaacctt ccagagacat caatcaaagg aaatttctta agataattca
     3241 gatttatttt tatccctctg ccaaactgtt cattttcttc attcaagtaa tctgatacat
     3301 tgactaatat acttccgttt ttattcaaag tttcatataa ttcctgatct gctgctgatg
     3361 caaaacgatt ggaataaata aaatccacgt ggtttttccc gatggttact ggataaaata
     3421 catgatagtc ctcaccagcg atttcttttg gtggttcatg ggaaacagct aactgtgaag
     3481 aagaaaaaac gatagttgct aattcgacat tggcaacggt caaaagaaga gaaaacaaca
     3541 atccaaatgt aaaaagaatg tattggatat agttgaacgc ttctcttaac gaagcgattt
     3601 tttcaactaa taaaagagaa atcaaagaga taccatatat tccgctgaac aagaaaacgt
     3661 acatctggaa caaataaaga aattgctcct tataatacat aaaaataaaa ccaccaatga
     3721 ttcctgcaat tatgaatgta ataatgattt tctttttccc caataaacac cacaatactt
     3781 gatgtaatga aaagtcatta ttcaacaata aactgatttc tttattcatc aataagaaaa
     3841 aggatgataa caaaagga
//
LOCUS       DADVXO010000172         3743 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.178, whole genome shotgun sequence.
ACCESSION   DADVXO010000172 DADVXO010000000
VERSION     DADVXO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.178"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            187..1902
                     /locus_tag="KB950_002646"
     CDS             187..1902
                     /locus_tag="KB950_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858808.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1905..3668
                     /locus_tag="KB950_002647"
     CDS             1905..3668
                     /locus_tag="KB950_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6858809.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1113 a          733 c          742 g         1155 t
ORIGIN      
        1 agactggcat gttttctcat tttctttttt ttaaaaaaaa tagtctattc aatttctaaa
       61 gcttcttcat aataaaactt tcatttatga aaaggcttgt tctcttttca ttctaaattt
      121 cctaagctac aatgtggtag tacttgtttt ttcaattgtg gacaaataaa taataggagg
      181 aatcatatga ttcagttatt acgttatgca aaagactatc gtaagcaaat cattttgggc
      241 ccgttcttca aatttcttga agctgtcttc gaacttgtcc tgcctttaat gatggcttcc
      301 ttgatcgata atggactaaa aatgaatgac cgaggaaaaa tcattgaaat gggcttatgg
      361 atggtcgcta tgtctgtgat tggtctgatt tgtgctatca tttgccaata ctatgcttca
      421 gtagcttcac aaggatttgg tacagagtta cgtaatcagc taatcaaaaa aatcaatact
      481 ttctctcaca acgagttgaa tcattttgga acagatacct tgatcacccg aatgacaaac
      541 gacatcaatc agcttcagct agctttagca atggtcattc gtttattgat cagagcaccg
      601 tttttaagta tcgggtcagt ggtcatggct tttgtgatcg actgggaagt aggtctgttt
      661 ttcttagcat tattgcctat tttctcaatc attttatttt ttatcatcaa aaaaaccgtt
      721 cctctctacc aaaaagtgca agaaaaacta gatcagctca atgaacacgt tagtcagaat
      781 cttagcggtg ttcgtgtcat ccgcgcattt gcgcagacaa aaaaagaaac agagaagttc
      841 gataaaagca caaatgatct agcgaacaat tatttacgtg tttccaatat ctctgcccta
      901 ttatcacctg ctacaacctt gatcatgaat gtcgggatca tctgtttgct gacagttggc
      961 gggatcaaag taaatattgg tagcttgcaa caaggacaag ttcttgcttt gatcaattat
     1021 atgaatcaaa tgcttttggc actgatcgtt gtttcaaacc tcgtcgtgat cttcacacgt
     1081 gccgaagctt caggaaatcg ggtgaaagag gtgctagaca cagaaaattc gattcttgaa
     1141 gctgaggtca gtactactcc cgatttttct tctgaagcaa ttatcgcttt tgaccatgtt
     1201 gacttccgtt atactcctga ttctggtctt tctctccaaa atattacatt tcagctgaag
     1261 cgaaatacag tccttggaat cattggtcca accggaagcg ggaaaacgac tttgacacaa
     1321 ttgattccgc gtttctatga tgtcagttcc ggtacagtta taatggacgg aatggatgtt
     1381 cgttcttggc agctggatca tctccgtcag caaattgctc ttgttcccca aacttctgta
     1441 ctatttaccg ggactattcg tgaaaacctt caatggggta agaaagacgc cacagaaaaa
     1501 gaatgttggg aagctctgaa aatcgctcag gcagaagaat ttgtccgtca gcttccaaat
     1561 ggtttagata caaaagtcat ggaaaatggg aaaaatttct ctggcggaca aaaacagcga
     1621 ctgacgattg caagagcttt gattgccaag ccagcattac tgattttaga tgactcttta
     1681 agtgcgttgg attatcaaac agacttggat ttgcgtaagg cgctacagac ttatcttggc
     1741 agtacagtga ttattatctc ccaacgtgtc cgctctattc aagaagcaca tcatatctta
     1801 gtgatggatc aaggtaagat tgctgctcag ggtacccatg aagaattact tgctcaatca
     1861 gcagaatatc aggaaatcgt cgcatcacag gaggaagaat agttatgaaa gtaaaaaaat
     1921 tatccttgca gacgctccgc cgattcattc cttaccttac tgcctatgca aaagacattt
     1981 ggctagctgt gatactagga atcatcaatg ggattgcaac agttgtgatg acgttacaaa
     2041 ttggtcaaag tatcgatcaa atgattggag aaggacaggt ccgcttcaat cgactgttcc
     2101 atttacttat tctattcggt ggtatcgttt taatcaacgt agtcagtcaa tggctcatcc
     2161 aagtgttaag caatcggtta gcttacttat ccgttgcaaa attaagaaaa gatgcatttg
     2221 ctcatctgaa tcgattgcca cttcgttatt atgatcaaaa ctcccacgga aatatcgtca
     2281 gtcgatttac aaatgatatg gacaatattt ccattgcaat ttctgcggcc ttcaatcaat
     2341 tattttctgg agtggcaatc atcattcttt cgtttatttt tatgatgcgt ctaagtccac
     2401 ttctgacatt ggtcgttctt tgttctacac caattatttt ccttgtgagt tggatcgttg
     2461 ctcgtgcttc acaaacaaat ttcgatagac agcaaaggat tattggggat atctcgggct
     2521 ttgtgaccga acgaatcggt aaccagaaaa tcgtgaaagc ttttcagcaa gaagaagcta
     2581 gtcaacagca atttgaactg ctaaatgctg atctatatga aaaaggacaa cgggcacaat
     2641 tttcttcttc cttgacaaac ccgtcttctc gttttgtaga tcaccttgct tatttatcga
     2701 ttggtttagt tggcggccta ttggcattga agagtggttc gtcaatcact gttggtgtga
     2761 tttccagctt tacgatctac tcaagtcagt tttctaaacc atttatcgaa ctttctggaa
     2821 tcacgacaca actgcaaacc gctttttctg gtctagaacg agcatttgat ttgatcgatc
     2881 agccagaaga aaagcctgat ttgccagatg cctatgcctt ggaacccaaa aatatcaaag
     2941 gaaaagtctc ttttgatcat gtttcttttt cttatgaccc caagcagcgt ttgatcgaag
     3001 atttcaattt tactgcttat ccaggtcaaa ccattgctat agtaggaaaa acaggggccg
     3061 gaaaatccac acttgttaat ttgctgatgc gtttttatga cacaacaaaa ggaacaatta
     3121 cagtcgatga ccatgacatc cgctctatcc aacgtgatac attacgtaaa agttttggaa
     3181 tggtgttaca agatacttgg ctatttgaca gtacattgcg agaaaatctt cgttttggga
     3241 atccggaagc aagtgacgca gaaatcaatg atgccctaaa agcctcttat atgtatgaat
     3301 ttgtagaacg tttgccggaa aaattagata ctaagattgg cggtcaaggg ttaaagatat
     3361 cagatggaca acgccaactg ttgacgattg ctcgaacgat gatcagtaat ccccctatgt
     3421 tgatcttaga tgaagctacc agctcagtcg atacattgac agagaaaaaa atccaacaag
     3481 ccttcttagc aatgatggaa ggaaaaacca gttttgtgat tgcccatcgc ctgtccacaa
     3541 tcaaaaatgc ggaccagata ttagtgatgg atcagggagc tattgtcgaa atgggcaccc
     3601 atgatgagct gcttcaaaaa gatggttatt accgacggct ctatcaagca caattcacag
     3661 cacaataaaa acaagcagtc ttctgggatg ggaagactgc ttgtttttta tttagcgtgg
     3721 atcgctaaac ttcttaaaat ttt
//
LOCUS       DADVXO010000173         3738 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.179, whole genome shotgun sequence.
ACCESSION   DADVXO010000173 DADVXO010000000
VERSION     DADVXO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.179"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            72..155
                     /locus_tag="KB950_002648"
     CDS             72..155
                     /locus_tag="KB950_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB6858810.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="KB950_002649"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="KB950_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB6858811.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="KB950_002650"
     CDS             complement(1280..2143)
                     /locus_tag="KB950_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6858812.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="KB950_002651"
     CDS             complement(2145..2417)
                     /locus_tag="KB950_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6858813.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="KB950_002652"
     CDS             complement(2434..2643)
                     /locus_tag="KB950_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBB6858814.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2742..3638)
                     /locus_tag="KB950_002653"
     CDS             complement(2742..3638)
                     /locus_tag="KB950_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6858815.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1148 a          654 c          579 g         1357 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt aatcacttgt gatttgtgat tctattcttt ttcattttct
     2761 aattgaatga ttcgctcaat catttcaaaa aatacgttct catacataga caaaactttt
     2821 ttgtcatagc ctttgtgttc tgtaatgcca tttttagacc aagtacttac tttattactt
     2881 cgcttgataa tatttcggaa aaccaaatta tcctctttat gttctttgta cagttcttcc
     2941 aggtttgatt ttatcgttgc gctgtccgta tcaactaaat aaggaacaaa accaatcata
     3001 tctagtccag ggttaaactg ttcttgcaaa tcaatcaaat aggaaatata gttttgaatg
     3061 ttgtttgtac tttcttcttc tgcttgtaaa gggatcataa cgtaatcact cgccacgatt
     3121 gcattatttg tataaacgct tggcgttggt acagtatcaa taataataag atcatagtca
     3181 ctttttaaag gtgctaaaag agtagcaagc aatctacttt cattttcaaa cgtccatgag
     3241 cgagttaatt ttggcagtaa catcaaatca aacgtgccag ggatcaagtc taaattatca
     3301 gtcaaatgaa taatagaaga agccaagttt ccatttttca agccttcata aaaattgaca
     3361 cgtggcaatt ctacctcaaa agtttttgct aagtcttttg tcaatgttgc ctgtaagtcc
     3421 ttatcgatca ttaaaacttt taaattcaat ttgtctgtca agtaagcaaa catagtcgat
     3481 aatttggact ttccaacacc acctttaaag taattattca agataataat tgcttcattt
     3541 ttattgttta aaatacgtct taattcttca agtattttta gtttttcctt ctccataact
     3601 acgccccatt ctttttttgt atagtaatat tgtatcatta agaatcacaa atcacaagtg
     3661 atttgtgatt aatcacttgt gatttgtgat aagtgatttg tgattaatat ataaaagccc
     3721 tctttaaagg gcttttat
//
LOCUS       DADVXO010000174         3735 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.180, whole genome shotgun sequence.
ACCESSION   DADVXO010000174 DADVXO010000000
VERSION     DADVXO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.180"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(573..1130)
                     /locus_tag="KB950_002654"
     CDS             complement(573..1130)
                     /locus_tag="KB950_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858816.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="KB950_002655"
     CDS             complement(1309..1920)
                     /locus_tag="KB950_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858817.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="KB950_002656"
     CDS             complement(2033..2290)
                     /locus_tag="KB950_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858818.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="KB950_002657"
     CDS             complement(2887..3165)
                     /locus_tag="KB950_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858819.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="KB950_002658"
     CDS             complement(3199..3450)
                     /locus_tag="KB950_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858820.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1125 a          765 c          505 g         1340 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagt
//
LOCUS       DADVXO010000175         3721 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.181, whole genome shotgun sequence.
ACCESSION   DADVXO010000175 DADVXO010000000
VERSION     DADVXO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.181"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..356)
                     /locus_tag="KB950_002659"
     CDS             complement(<1..356)
                     /locus_tag="KB950_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858821.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(616..1164)
                     /locus_tag="KB950_002660"
     CDS             complement(616..1164)
                     /locus_tag="KB950_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858822.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1165..2022)
                     /locus_tag="KB950_002661"
     CDS             complement(1165..2022)
                     /locus_tag="KB950_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6858823.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2308..2594)
                     /locus_tag="KB950_002662"
                     /pseudo
     CDS             complement(2308..2594)
                     /locus_tag="KB950_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2584..2913)
                     /locus_tag="KB950_002663"
     CDS             complement(2584..2913)
                     /locus_tag="KB950_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6858824.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2990..3262)
                     /locus_tag="KB950_002664"
     CDS             complement(2990..3262)
                     /locus_tag="KB950_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6858825.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3262..3525)
                     /locus_tag="KB950_002665"
     CDS             complement(3262..3525)
                     /locus_tag="KB950_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6858826.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1402 t
ORIGIN      
        1 ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac taataatact
       61 tgatctcgct tattataatc gctttcattt atcctaagag tcaaatcttt ttccaccata
      121 acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct ttgtgtagcc
      181 tttaaactga caattagctc attgggtaca tactctattt gagagtcatc taattggctc
      241 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      301 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcatctac
      361 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      421 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      481 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      541 cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      601 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      661 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      721 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      781 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      841 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      901 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      961 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
     1021 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1081 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1141 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1201 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1261 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1321 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1381 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1441 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1501 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1561 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1621 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1681 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1741 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1801 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1861 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1921 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1981 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     2041 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2101 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2161 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2221 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2281 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2341 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2401 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2461 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2521 ttctttcaat ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg
     2581 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2641 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2701 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2761 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2821 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2881 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2941 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     3001 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     3061 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     3121 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     3181 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     3241 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     3301 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     3361 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     3421 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     3481 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     3541 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     3601 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta
     3661 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
     3721 a
//
LOCUS       DADVXO010000176         3720 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.182, whole genome shotgun sequence.
ACCESSION   DADVXO010000176 DADVXO010000000
VERSION     DADVXO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.182"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            15..1004
                     /locus_tag="KB950_002666"
     CDS             15..1004
                     /locus_tag="KB950_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6858827.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            986..1447
                     /locus_tag="KB950_002667"
     CDS             986..1447
                     /locus_tag="KB950_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6858828.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1497..1961
                     /locus_tag="KB950_002668"
     CDS             1497..1961
                     /locus_tag="KB950_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6858829.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1975..2724
                     /locus_tag="KB950_002669"
     CDS             1975..2724
                     /locus_tag="KB950_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6858830.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2724..3554
                     /locus_tag="KB950_002670"
     CDS             2724..3554
                     /locus_tag="KB950_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6858831.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1251 a          493 c          720 g         1256 t
ORIGIN      
        1 taatacttat tagagtgagg gataaagaaa tgagacaact acacaaagaa cagattgaaa
       61 cagcaatggc agcgttaaag gaaaagacaa ttgatcatgt atattttaca gcatgtgggg
      121 gttcacaagc cgttttaatg gctggtcaat atctatttga taaagaatta actgtacctt
      181 gtcatgttta tactgctaac gaatttaact atgctatacc aaaaggatta tctgaaaatt
      241 ctttagttat cacttgttcc cattctggca caacaccaga aactgtagaa gcaacaaaaa
      301 aagcaagaga atttggagca atgactattt gtttatctaa tgaagaagga tctcctctat
      361 gggaagcagc tgaatatcct gttcactatg attggggtaa agatgttgat gctagtgatt
      421 taaataaagg aattctttat ggtttattat ttaatatttt acttgtttta gatggctcta
      481 aaaaatggga aacttgcttg aatgaatttg ataagttaaa tgatcttgtt attgctgaaa
      541 agagcaaatt tgaaataact gctaaagaat ggggtaaaaa ccaaaaacga gataaaatta
      601 tttacacaat tggtagcggt atcaattacg gagaaattta ctccacagca gcttgctggt
      661 tcatggaaat gcaatggatt aattctaatg ctattcattc tggggaattt ttccatggtc
      721 cttttgaaat cactgattat gatgtaccat tcatcttagt gaaaagtatt ggagatacaa
      781 gatttttaga tcaacgggta gaagacttca ctaaaaaatt cagtaataaa ttaatggttt
      841 tagaccaagc tgattttgac ttagaatcta tagatagtgt agcgaaagaa tatattgcag
      901 ctgtacttag tggcgttgtt attcgccaaa tggtagaagc gattgctttt gaaagaggcc
      961 attccttaga tgttagacgc tatatgtggc aaatgaaata ttaattaata ggaggttgca
     1021 aaagatggca aatcaattat ttcacattgt gattacaaca cattctaccc tttgtgaggg
     1081 ttatttaaag gcaacaaaat taattttaat gaatgacgcc acaggtatta gtgctgttcc
     1141 atttaaagaa aatactagtc cagatgattt tgaggagagt atgaaagaaa taattagtca
     1201 gcatcatttg caacctctaa ttattttaac ggatttaatt ggaggaacac ctaataatgt
     1261 ttctattaaa cagttgaaca acaatagaat tcaaattatt gctgggatta atttacctct
     1321 tttactggag ttattaatgg cccagtcagc tggtaaatct atagaagaaa tagattttaa
     1381 ggaattactg aataactctc aaaaaagtat ggttcatatt aattcgcttt taaaagatgt
     1441 cagttaaggt ggcaatttct attctataaa ttttaaaaat tgaaggagga aatagaatga
     1501 ttaaattagt gcgtgtagac cacagattac ttcatggaca agtaatattt tcttggacta
     1561 aacagtatga tattaaccat attattgtgg ctgataacaa agttccagga gatccaatgt
     1621 ctgtgatggc tttgacaatt gctaaacctg ctgattgcaa attagacatt gttaaaatta
     1681 gtgatgttaa agggattatg gaaacatccc caaatgataa ttacatgatt ttaattaaag
     1741 gacctaagga agcttttgaa ttagttcaaa atttaccaga aattaaagaa attaattttg
     1801 gcggtgtgtc taagaaaagc gagtccaaac aatatggaaa ggcagtcttt ttaactcctg
     1861 aagaattaga agccacaaaa aaaattcttg atcaagaagt gaaaatattt gtgcaacaag
     1921 tcccatcttc aagtattgaa aatgtagatt ttagtaagta aggggggaaa ataaatgtta
     1981 ttaaaagcca ttttattagg tttagttggt gtggtagctg ttattgactc caggttaatt
     2041 ggtcgacaaa atattggtag accactaatt ttgagtacac ttgccggact tgtgctaggt
     2101 gacttgcata cagggattgt tcttggggca tctttagaat taatttctat gggattcgtc
     2161 tcaattgggg cggctggacc tcctaatatg caacttggta gtattattgc cactgctttt
     2221 gcaattttaa ctaatagtag tacagaaaca gccttaacaa tagctatccc agtggctgta
     2281 gctggtgaat ttatgagtat tatcatgcgc atgataattg ctcaatttgg acatgctgca
     2341 gataaagcca ttgaaaataa taaatttaga aaagctcaaa ttattcatat ttattggtca
     2401 tttattttta atgcttttgt ttactttatt ccaatctttt taacagtata ttttggtgct
     2461 gacgttgtgg cagatttagt agataaaatt ccagaaatga ttacagacgc cttgactgtc
     2521 tctggaaata tgctttccgc attagggttt gcaatgctat taagcacaat gttgagtaaa
     2581 aaattatatc cttactttat ttttggattc tttattgttg cctattcagg tctttcacta
     2641 attggcgtga caatttttgc agctctgatt gcctttgtaa tggatcaagt taaatatgga
     2701 aaaagagaag aggcgcatgg ttaatggaaa atagtacaga aaaaaaacgt ttaatcacaa
     2761 aaaaagatct taataagatg tgtcttcgtt catggacaat catgtgttcg tggaattatg
     2821 aaagacaaat gcatatggga tttatgtatg ggatggcgcc aattttaaat aaagtttata
     2881 aaaatgatga agagaaaaaa agagaagctt atactcgtca tatggcattt tttaactgta
     2941 cgcctcaaat gacttcattt attatgggac tttgtgcttc tatggaagaa caaaatgctc
     3001 aagcagaaga aggaaacttt aatactgaat ctatctccat gattaaaacc agtttaatgg
     3061 gtccttttgc tggaattggt gattcatttt tccaaggtac tttaagaatt attacatttg
     3121 ggattggatt gtcttttgct aagcaaggta gtattttagg accgatttta gctgttggcc
     3181 tttttgctat accttctatt ttagctgttt tttacggcgg taaattagga tatgagtctg
     3241 gcaataaata ccttactaaa ttgtatcaag aaggtcttat ggatcgagta atgcaatttg
     3301 cttctatcat tggtttggct gtagtaggtg gaatggtggc tagcatggtg ccaattgtca
     3361 cccctttaaa attcactaca gggggaacag aacttgtttt acaagactta cttgattcca
     3421 ttattcctgg attattacca cttgtatgta ctttgggaat atataaattg attaagaaaa
     3481 atgttagtac aaatatgctc ttattaggtt gtattttggc agggtttgtt ttaagtataa
     3541 taggtatctt ataatgatta aggctctaat ttttgatatg gacggagttt taattgatag
     3601 cgagattatg tatcaaaagt gggtatttaa ttttttaaaa caagaaaatt taagctatcc
     3661 tttgtattct tatcaaaaaa agattggaac tacagcaagt atttttgatg atatagaaaa
//
LOCUS       DADVXO010000177         3657 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.183, whole genome shotgun sequence.
ACCESSION   DADVXO010000177 DADVXO010000000
VERSION     DADVXO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.183"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(365..1069)
                     /locus_tag="KB950_002671"
     CDS             complement(365..1069)
                     /locus_tag="KB950_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858832.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1256..1501)
                     /locus_tag="KB950_002672"
     CDS             complement(1256..1501)
                     /locus_tag="KB950_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858833.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1518..2369)
                     /locus_tag="KB950_002673"
     CDS             complement(1518..2369)
                     /locus_tag="KB950_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6858834.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2535..3539
                     /locus_tag="KB950_002674"
     CDS             2535..3539
                     /locus_tag="KB950_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBB6858835.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1148 a          622 c          706 g         1181 t
ORIGIN      
        1 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aaagtgttag tagaaaatcg
       61 ctttcgattt tccaccaaca caaaatatta tagaaccgct tttttctcta tcagaaaata
      121 tgtttattat tagaattttt tatagttaag gttaggcatc ttaagaaatc aggcaactcc
      181 aagatatctt caggtagaag agaagtcgcc taacattgta ggatcatagg tgatttgttg
      241 gacaggtcaa gctgatattt gagcctgatg ttagaaggaa tttcttttta cttgctgtag
      301 aaattattat atgactaaaa aggctgggat tttaaatccc agccttttat gacaaataga
      361 atttttatcg atcagtaatc gctgtcgctg caaattgttc gatcaaagga atcaaagtca
      421 atttgacttt gtatttccgt tcgagtgctt tttcgataac gaactcattt tcagtagtca
      481 tagaaatagt tgctaattta cgaggttcat cagcattttt ttcattaata actacttcat
      541 gttcattttg ttctgtgtaa aatttaggat actcagcaac ggcttttgaa aaatcagcaa
      601 aacttaaagc ggcattcgta tcagttgagc gtgtattttc agttgtgtcc gcttttggtt
      661 tttccgtttt ttcttctggc acactgactg tttcttctgt aactggtgtc gcaacagctt
      721 gcgcagttgg ttcatcgttt ttcaatcgtt gatcattcca gtaagacacg atttcaataa
      781 gaagattgtc acggaatcgt tcgaaaatcc cgttttctcc tgaacgaatt ctctttaata
      841 cagtattgaa gttgtcatcg attggcacgt tgatccgtgt atcttcattt gttttcccag
      901 ctaatccgat cgttaagatt tgacggtttt tgggatcaag catgacttgt aacggcaaac
      961 cataacgtcg caagctctct tcttctagag aacggaataa ttgtcctctt acaccatcca
     1021 atgaaggacg gaattgattt aataattttt gtttctgttt tttcttcata tgttctctcc
     1081 ttcgctcata ccctttcgat tataagaaaa aaacagccaa atgaaaagga gtaatgcaaa
     1141 aaaacttgcg tatacgcaaa ttacaacaga aaaatttgcg tattttcccg ttttttgaaa
     1201 gaaaaataaa aaatggaacg gattatgtat atatacataa tccgttccat tctttctatt
     1261 ttttcggtat caaggcaaaa acaggtagtt caggtgaata aatgacttca tgttcgtgcc
     1321 agggtttgac cattagcgaa tgatcgaacc gttttttcag ttcttcgatg atttgatcat
     1381 gtgtccagtt tcttgcagga aaatgttgga ttttctctgc atcgatcaaa aagacaaaat
     1441 cggaatgaaa agtctgggta acttcttccg agagagaatt caagcgttcg attggatcca
     1501 taaaatcacc tttcgactta aaattgggta gtatctgggt cttttgcttt gccgactaca
     1561 ccatctaact gatcgatttg tttcatttct tcttctgtta attcaaaatc aaataattca
     1621 gtattttctt tgattctgct tggtgtcact gacttaggaa gaggaaggaa ttcgtgttgc
     1681 agcgaccaac gcaaagcaac ttgtgcaatc gtcttgtcat gtgcttcagc gatttgtttc
     1741 atttcaggca catcgaagat tttaccagtg cctaatggac tatatgcttc gaggatcata
     1801 tcatgttttt ttgcatactc aactacagtt gtctgtaatt cgcctggtgc aaggaaaact
     1861 tgattgacca tcggcataat ttttgctgtt tttgctagtg tatctagatg gtggggtaag
     1921 aaattgctca caccgattgc tttgatttta cctgcttcat acagttcttc aaatgctcgc
     1981 catgtatctg catttgcttg ttcccaatta tcacgaaaag caacagggtt tggccagtgg
     2041 atcaaaaaca gatctaaata atctgtttgg agcttcttca atgattcttc aaaagaagac
     2101 atcacaagtt catagctgtg gtttgcattc cataattttg ttgtcaagaa tatctcttct
     2161 ctaggaattc cactttcctt aatcgcttgg ccgacgcttt cttcattctt gtatccttgg
     2221 gcagtatcga tatggcgata acctgcagcc agcgcttctt taacagagga aacagcgaca
     2281 tccccatcag gtgtttgcca tgtgccgaat cctactaccg ggatttcgta accatttgat
     2341 agacgataag tgcttgttaa tgaattcata ctttcagctc cttttttatt cgttcaacta
     2401 taagtgtagc gcatttttcg aaaaaaagaa tccattttgt ttctactctt gaaatttttg
     2461 tttaaaagaa aatgattttg attctggtat ttttgatttt ttttccgtaa actaagacag
     2521 aagggaatga gaaaatggat atctatttaa aaaaagcaat tctgcatatt attgacagag
     2581 aaactggttc tccagtcttt tcacaaaaag agctggatct gacaaaagaa tatattcgtg
     2641 attttcttca aaagaagatc caaaaaatct cttctgctca aacaaaaact ggacaactag
     2701 cagaagaaag catgatcgtt gaatcactta aaatcgctca agaaaatttt gtggaagcaa
     2761 gtgaagcaat cgttcagcgg tggttcgata tctaccaaga aagtgaagaa gcacctagtg
     2821 cagatgtatt tgttgtcttg tatgaattag atacagtgat gtacttatct ttattgaaag
     2881 tcaattatcg tgaggcttac actcactttg ttgaagcaga cgaagcaggg atcgacaacc
     2941 gcttgatttt gaatcgagca attctagggg cgaaaacaca aaaagcagat gaagcattcg
     3001 ctgtgaattt gactgattgg acctacgaat taattgaaaa aaaatatgag ttttctggca
     3061 aaaaagagct ttatttttct ggaagagtga tcgaaagtgt tcctgcacct tctttagaag
     3121 aaaatgtaaa agtgatccaa aaagtagcca agcatcttgg aaaaaaattt gagacagaag
     3181 aatttgatat catggctgat ttgaaagaag ctgtctatga tacgatagaa gaaaaaggtc
     3241 ggttggatca tgagatgatt gcggaaaaag tcttcaagga aaacatcacg gcaaaaatgg
     3301 cttttaaaga agaagtacaa gagcaaggat ttgtcccaga agcacctcct gtaaaagaag
     3361 tacaagaaat ttctgagaaa aaatttggaa aacaaaaatt caagctatca aacgggatcg
     3421 aactgatcgt accagtagat gtatatcgca acccagattt gattgaattt gtgaataatc
     3481 cggatggtac gatatccgtg atgatcaaaa atgtggatga agtattgaat cgtttataaa
     3541 aatgactaga atattaggtg ttcaagtacc agtgatcagt taaatgaatt gtgtcaagag
     3601 aaggatcgga aaagacgaat ataacaaaaa aagagcgtga gacaaaagta aagaaca
//
LOCUS       DADVXO010000178         3646 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.184, whole genome shotgun sequence.
ACCESSION   DADVXO010000178 DADVXO010000000
VERSION     DADVXO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.184"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            9..761
                     /locus_tag="KB950_002675"
     CDS             9..761
                     /locus_tag="KB950_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6858836.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            758..1672
                     /gene="pfkB"
                     /locus_tag="KB950_002676"
     CDS             758..1672
                     /gene="pfkB"
                     /locus_tag="KB950_002676"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBB6858837.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1690..3621
                     /locus_tag="KB950_002677"
     CDS             1690..3621
                     /locus_tag="KB950_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6858838.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1172 a          659 c          837 g          978 t
ORIGIN      
        1 tcgtgaaaat gcttacagag gaacgcagac aaaaaatcct agacttgctt gaacagcaga
       61 aaatcgtgaa atcacaagat cttgtaaacc ttttgaatgc ttctgaatca acgataagac
      121 gtgatttaca agagctggag aatagtgggt ttctagaaag agttcatggc ggagctaaga
      181 aagagcaatt attgaatttt gagcagaata tgagtgaaaa atcactcaaa aacgttcacg
      241 aaaaacagca aatcgccaaa tcggcagctg aaaccattaa tgacgaagat gtcatttatt
      301 tagacgctgg ctctactacg ttagaaatga ttccttttct gcaaggaaaa cagatcacag
      361 tagtgacaaa ttccgttcaa catgcggcaa atttagttga tttggagctt gctaccatta
      421 ttttaggtgg aacgataaag ctggcaacta atgctgtttt aggatctaat gcgatagaac
      481 aactaaaaaa ttttcatttt aataaagcat ttatgggaat gaacggtgcc catcttgaac
      541 aaggattcac gactccggat ccagaagagg cagctgttaa acgtttagcc atgaaaaatt
      601 ccgaagagac atttgtattg attgatcata ccaaattcaa ccagttgtcc ttcacctctg
      661 ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg tccattagaa ttcgttaatg
      721 aattccgtga aaagacaaca attaaggagg caatacaatg atttacacag taacacttaa
      781 tccatcaatt gattatatcg tacgcgtaga tgggttaaaa ctaggcgatt tgaaccgaat
      841 gaaggaagac ttcaagttac ctggaggaaa agggatcaat gtttcccgta ttttgaaacg
      901 gatcgattct caatctacag cactagggtt tttaggaggg ttcactggcg aatttatttc
      961 gaagtggctt gaaaaagaac aaatccagtc tttgttcaca accgtaaaag aagatacaag
     1021 aatcaatatc aaactaaaat cagacactga aactgagatc aatggcttag gcccatctat
     1081 ttcagatgaa gaaatcgatg agttgaagca agcattagga acagtaggac aaggagatat
     1141 cgtcgtattg tcaggaagcg cgccagctag cctgcgaaaa ggattttatc aagaactgat
     1201 tgatatcatt cgagaaaaag gtgcagattt cgtgattgat acaacaggtg atgacctaaa
     1261 aaatgcactt gttaagaaac cgttgttgat caaaccaaac aatcacgaat tagctgagct
     1321 ttacaacaca accttccaat ctgtagaaga aatcattccg tatgggaaaa aattactagc
     1381 tgaaggggca cagaatgtgc tgatctcaat ggctggagac ggtgctttgc tcttcacgga
     1441 acaaggaact tataaaacga atgtattagt tcgcccattg aaaaattcag taggtgcagg
     1501 ggattcaatg attgctggct ttattggggc ttacagccgt acgaaagatc caatcgaagc
     1561 ctttaaatgg ggagttgctt gcggaagcgc aacagctttt tctgatgact tggcagctcg
     1621 cgaattgatt gatgagttgc tgccagaagt tgaaatcaca aaaattgatt agtttcaagg
     1681 aggataatca tgaatatcaa ggatttaatg gtcaaagaag cgatgatcat ggatttgcaa
     1741 gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa aaatgtttga tgccggccgg
     1801 atttccgata tcgaaacata taaagaagga attctagccc gcgaagcaca aacttctaca
     1861 ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca gtgcagttaa agaagcaaca
     1921 gttttattcg caaaaagcca aaaaggagta gattatgaag ccttagacgg acagccaact
     1981 tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg acacacattt gcaagcatta
     2041 gcagcactat ctcgattgct gattgatgca gatttcgtag gaaaattaaa agaagcaaat
     2101 acaccagaag aagtccaaga cttgttccaa acagctgaag agcaaaaaga agcagaagaa
     2161 aaagcagaac aagaaacaca agctgcagaa gcttctgctc atccagaaag aaaattcgtt
     2221 gttgccgtta cagcttgtcc aacagggatc gctcacactt acatggcaga agatgctttg
     2281 aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg aaacgaacgg ttcagaagga
     2341 atcaaaaacc gattgactgc tgaagacatc gcccgtgcag aaggtgtaat cgttgcagca
     2401 gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac tgatcaatcg tcctgtcagt
     2461 gatggtatcc gtaaaccaga agagttgatc aatctagccc ttagtggtac tgcaccagta
     2521 ttccatggtg atggaaaaga aagcagttca gaagaaggaa atgcggatgg tactatcgga
     2581 caaagaattt acaaagattt gatgaacggc gtatcacaca tgttaccttt cgttattggt
     2641 ggaggaatcg ctattgcgct ttctttcatg atcgaccaat ttattggcgt gccacatgat
     2701 caattatcaa atctagggaa ttacaatcaa gctgctagct ggttcaacca aatcggacaa
     2761 gctgcatttg gtttcatgct gccggtatta gcaggattta ttgcttcaag tatcggtgac
     2821 cgccctggtt tgatcgttgg atttgctgct ggtgcattag ctaatacagg cggtgcagga
     2881 ttcttaggtg cactgattgg tggattctta gctggttatg tcatcgtatt tttacgtaaa
     2941 ttattcaaga acttaccaaa atcattggat ggaattaaaa cgatcctctt ttatccagtg
     3001 tttggcttac tgattactgg tttcttgatg ttgctggtca atgtccctat gaaagcaatc
     3061 aatgatgcat tgaacggttt cttgacaagt atgagtggtt cgaatgctgc tttacttggt
     3121 gcattattag ctggaatgat ggcggcagat ttaggcggtc caatcaacaa agccgcttat
     3181 gtgtttggta cagcaacttt agctacgact gttgcaactg gcggaagtgt cgtgatggct
     3241 tctgttatgg ctggaggaat ggtacctcca ttggcaattt tcgttgctac acgtttgttt
     3301 aagaataaat tcagcaaaac agatcaagat gctggtttga cgaacatcgt gatgggacta
     3361 tcgtttgtca cggaaggatc gattccattt gcagcagctg acccgattcg tgccattcca
     3421 agttttatta ttggttctgc tttaactggc ggattagtcg gagctttcgg aatcaaatta
     3481 ctggctcctc acggcgggat tttcgttgta ttcttactga gtcacccggt catgtatttg
     3541 gtgtttattg ctatcggagc agtcgtatct ggtgtgattt atggtttatt gagaaaagct
     3601 cctgcagtag cagaagcata gaaataaaaa agaagaggct gggttt
//
LOCUS       DADVXO010000179         3589 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.185, whole genome shotgun sequence.
ACCESSION   DADVXO010000179 DADVXO010000000
VERSION     DADVXO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.185"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..418
                     /locus_tag="KB950_002678"
     CDS             <1..418
                     /locus_tag="KB950_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBB6858839.1"
                     /translation="LYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVT
                     DKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASPTI
                     KGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1033..2073)
                     /locus_tag="KB950_002679"
     CDS             complement(1033..2073)
                     /locus_tag="KB950_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBB6858840.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2422..2748)
                     /locus_tag="KB950_002680"
     CDS             complement(2422..2748)
                     /locus_tag="KB950_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858841.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2735..3538)
                     /locus_tag="KB950_002681"
     CDS             complement(2735..3538)
                     /locus_tag="KB950_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6858842.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1093 a          628 c          531 g         1337 t
ORIGIN      
        1 tttgtatcga gccatcgatg cagatggttt aaccttggat atttggttac gtaaaaaacg
       61 ggacacacaa gcagcctatg cttttcttaa gcggttagtg aagcagtttg atgaaccgaa
      121 ggttgtagtc acagataaag caccatcaat tgctagtgcc tttaagaaac taaaagaata
      181 cggattttat caagggacag aacatcgtac cattaaatac ctgaataatt tgattgaaca
      241 agaccatcgt ccagtaaaga gacgcaataa attctatcga agtttacgca ctgcctcacc
      301 cacgattaaa ggcatggaag ccatccgagg attatataag aaaacccgaa aagaaggcac
      361 tctcttcggg ttttcggtct gtactgaaat caaggtatta ttgggaatcc cagattaaat
      421 catagatacc gtaagggatt ttattcttta tttaaaactt tgcaacagaa ccgtccgttg
      481 cgggtaatac cgtaacactg aagggcacgc attaaggcgt catcttaatg ctttcctgta
      541 ggacactgac ggagattacc agtctcccga gccttacagg ggcctttctt tttttgtatt
      601 ttcaattatc acaatttatc gtccaaaagt gcagaacgaa aatcaattca tcacaagcat
      661 atcagacaat taaatttaaa acaactaaaa atatacaaaa aagtgtaatt gaatattagc
      721 agcactgcct gaaaagaagt aatatcaggc ttttcagctt ggggaaataa gtgaattacc
      781 tctcttcatg aattattata actcaaaaaa gcacatatgc aaacactagt tccctatttt
      841 tttgaatgaa aaataactaa aatatctaac gattatgact ttaaaaccta cgtttgcgat
      901 tgttttcata aacttttaat ctaaaaactc cggaataata gctatttatt tgtaacattt
      961 gtaataaaat taaaatcata aaaaagttct aatttaaaat tgatcatttt tatagtgatc
     1021 gctttttatc aactactcta gccaattttc cgtactaact gttggtaatt cgttaaatgt
     1081 ttctctttgt tcgtttgcaa gctttgccgc aatagctttt tcgacaaacc aattttttac
     1141 ataaacgaaa attagattct gcaaattgtt tactttttca gttttttgtt tttgataagc
     1201 tttcagcaaa gtcatatata atcctttgtc agcatcaatt ccataagatt ccagttcttc
     1261 ataaacaaca actgtttgct ctaattcttc agcttttttc tttgcattat gaatagtttg
     1321 gacggttttt tgagcgtctt cgtaagtact agaaaatgtt tttatcaagt ttaatacatt
     1381 ttctggtaca aatgtattta tactgtcctc tttcatgatg tcgacgaagt tatctagtaa
     1441 aacttgattt tgtaactgat cagtttcagt gtctattatg tgtctattag tgtctataga
     1501 attagaatag aatagattgt ctgcgatttt cgcaattacg gttgagtcct tgatatgact
     1561 ggattttgcc gtatttgcga ttttcgcaat tacggtggta tcattgattt ctcgcggttt
     1621 tgccgtattt gcgattttcg caattacggt ggtatcagta ttgtcttcct cttccatgat
     1681 tttaaagacg tcttgattcg ttacttctgg cttgcctaag tacaataaat taggcatgga
     1741 ttcaatctta ccgtcacgtt tatttactct tcctctcttc tgtttcagaa gacctgcttt
     1801 ttctaattcc tttttgattt tagatacttt accttcgcgg cattgtaaca atttcattaa
     1861 ttcttcaact gtgaatatga aaaatatatt atcatcagaa tctacccagt tatttttgac
     1921 tgaataatca aatcgatctt taagtagcat ataagcaatt tttgcatcat ttgatagatc
     1981 catgtatttt tcgttagtaa aaaatacctt aggcatttga taatattttt cacgataaat
     2041 ttcttttgat ttgtaataat taaaatcatt catagccaat cctctttctg taggatagcg
     2101 ttttgttcaa aactatgtta caatgaatta aacaaatata tctatccact atatattttt
     2161 ctactgctga taagtcgcca aactttgtta aagcagtagt ctttttttat ctttttttag
     2221 tttaccttaa aaaataaatt aaagcaaacc gctctatttc aaggtttaag ctcatagtaa
     2281 ctatatcacg atattacaat aatattatat cacgatatcg taatattaca atatgaagat
     2341 aagcgactta tcagctttga tactactgat ttttataaaa aaaatatcac gatattatga
     2401 tatcgtgata ttgaatgtaa attaaatttt attttgtttc tttgctagtt tatatccttc
     2461 aatcaagtat tcttcaggta tggactttaa tagcgcatct tctataacgt ctggtttact
     2521 tttgtctgat tcagcaatag tcgtccaata tttgagtgct gacattagaa ttggatttat
     2581 acgaatgcta tctgctgttc ttacactact tccttgatct tttatgactt tacttatatt
     2641 tttcttggtt aattcgattt cttctacttc ttttgattta aaattctttt tatttttatt
     2701 tcctagtaat ctacttactg cagcttgact gcgattatct tctgccatca aaatcaccaa
     2761 cttcttttaa atattcttct gtaaatgctt tatagtcatc ttcagctgtt gagggtttat
     2821 taaaattgat tagatctgat ccagcagctt gcgcttcgtc aacggttatt gtcctacgaa
     2881 tataagtttt gaaaattttt gcaccaatga ctctagaaat ttcggttgct gcagacgtca
     2941 tttctttaga aatgttataa cgcttattat acttagtaaa tagaactcct cgtacttcaa
     3001 gcttgtcgtt cgtatattct tttaccgttt caatagtttc acttaacttt attagtcctt
     3061 ttaatgagaa atacgcagct tcaactggaa tgataatttc atcagaaaca gtaaatgcgt
     3121 tgactgtaag cattcctaaa cttggtggac aatcaattat aatataatca tagttgtcct
     3181 tgattggttg aagtactctt ttaagcctgt gttcacttcc tacttgagta aattccctct
     3241 ctgcagaaga aagggctatg tcggctggca aaacatcaat tcctttatag tccagaatag
     3301 catctgtaat gtcagcctcc ccttttaata cttcataaat tgtaggttgg tttgttccat
     3361 ctatatcgaa acctacatta tctgtagcat ttccctgagg atcaatgtcc actaaaagta
     3421 catttttacc gtaaacaaat ttcaaacatg ctgctaaatt aacgctagtc gtagttttag
     3481 aaactccacc tttttgattg gctactgaca atattcttgc cattaaaata tcccccattc
     3541 atcttatatt ataatataat tatatcataa tattgtgata ttgcaatat
//
LOCUS       DADVXO010000180         3584 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.186, whole genome shotgun sequence.
ACCESSION   DADVXO010000180 DADVXO010000000
VERSION     DADVXO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.186"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..417
                     /locus_tag="KB950_002682"
     CDS             <1..417
                     /locus_tag="KB950_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858843.1"
                     /translation="TILIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMS
                     DEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFID
                     VNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            431..1120
                     /locus_tag="KB950_002683"
     CDS             431..1120
                     /locus_tag="KB950_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858844.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1245..>3584
                     /locus_tag="KB950_002684"
     CDS             1245..>3584
                     /locus_tag="KB950_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBB6858845.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRS"
BASE COUNT         1212 a          470 c          755 g         1147 t
ORIGIN      
        1 acaatattaa ttgggggcta ttttcttttg aatttagatt ctgttaattc aaacgctcaa
       61 ataaaagatc aaagagtagc tgaacataat aataaaaaaa tgagtttgac caaaaatatc
      121 tattctatgt cagatgaaga attgtataaa gaatttggtg atttatccgg ggaacagtta
      181 gaagaaagta ttccaggaat tagtgactca gataaaattg ttgtcttgtc tgctggtggg
      241 caattaatgg gagatggcaa atccgaatta gttagttata aacaaaatca cagacagttt
      301 attgatgtta atactgattc caacgctatt tcgattaagg atctaaaaga gcttttaaat
      361 agtaaaagaa atgaattaaa gcagataaaa gaaactaatt cagtggaatt agagtaaagg
      421 aagattcatt atgaaaaaga tttttggtgt ttctgttggg ctacttatga tgttaacatt
      481 tggaaatgca gtatatgctg ctgagaaaga agttatttca gatccagagc agtacgcctc
      541 acgatacttt gctgattttg aagaagaaga taaagtactt gcattgccag aaggtggttt
      601 tttaaaaggg gaagctaaaa ttgtatctat ggataatcca gatataattc aaggttcata
      661 taattcagaa actgatcctg cttcagtttc agtgacagaa gcaaaaaaaa ttgttgctga
      721 aaaattcaaa aatgatattg aaactactcc aattatgcct cgaggagcat ttccacccga
      781 tgttggtact agcggacacc ttcttgtact agcaggagga ggaagctata aatcacagcc
      841 atttagtggt gcgggatggc gttttggagg tatgggtgta aaaccagctg atggtacagg
      901 caacttttta cgttggaaat cctacattga cgatggaaga gtaggaaatg taaatcaagc
      961 caacgcaact aaatctggtt ctattcaagg aatggcattg tatgctcgtg aaggattcaa
     1021 atactttgca ccaaacgggc ctggttggac aatgaatata ggtatttatt acacttataa
     1081 tcctgttgca ggtacgtatt atgttgttca aaatatttaa taaaacagca caaagagatt
     1141 tctttgtgct gttttattat ttttagttgt tgacaaacac taaaatataa aatataatca
     1201 acacataaac gaacgtatgt tcggcaagaa agcaggggat ttttatggat gagatggttt
     1261 taagtactca gaaatggcta aacaagaaat acagtaatgt tacagggttt gataaggttc
     1321 cagaaaatgg gcgaacaggg tggcctacaa tctatggact tattgaagga ctacaagtag
     1381 aacttggtat tactaatcta gtagctaatt ttggacctac cactgaaaaa atgtacgata
     1441 atcaggttac tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt cttattcaag
     1501 gtgccttttg gtgtaagggg attaatccag gtggcttcga tggggtatat actccatatt
     1561 tagatactgc tgtaaaagag ttgcagactg atgcagggtt taatggaagc gctgtaactg
     1621 gagttttgga tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt gttcttgttc
     1681 ccggcggaga tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat tatttagaat
     1741 acacgggaat aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa gcattaattt
     1801 ttgcattaca agctgaacta ggatattctc ccagtgaagc aacagggtct tatggtaatg
     1861 ggacaactag taaaacttat cctgtgtctg aaggtaactc gggaaactat gtaagaataa
     1921 ttcaatatgg attatatgtt aatggttatt ttgaagacgg tacattcgat ggtattttca
     1981 ctaaatatat gggtttagaa gtactggcat tccgaaaatt tatggtattg cctgaatata
     2041 ctgaaatagc agatgttgtt gtaataaaag gactattgtc tagcgctgga gatattagaa
     2101 gatctgcaga tggcgcagat acttctacgc aattaactag atctcaaatc caaacattgg
     2161 ttgataatga tataaaaata gttggtagat atcttactgg tactgttgga attggaaaag
     2221 atgaacgaaa taagtatcta actacagagg aattgaataa tattttttct aaaaatttat
     2281 ctgtgtttcc tatctatcaa gatggaggtg cagcattagc ttattttact tatgatcgtg
     2341 gcttatcaga tggtaagaag gctatagatg cagcaaaaaa tctaaatata ccattaacaa
     2401 cagttattta ttttgctgtt gatctagaca tgttagggga agaaattctt gcatatgctg
     2461 gagaatattt taagggggta tctgctgtaa tgtcatatag cgggtatcaa acgggtgttt
     2521 acggaacaag aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg tttgcatttg
     2581 tttcagatat gtcgactggt tatagtggga atttaggttt tcctatgcct agacaatggt
     2641 cttttgatca atttgttgaa tttaccatag gaagtggcaa cggagctgtt ggtatcgatt
     2701 taatcgctac atcgggtaga gatagcggat ttaatgagtt atcaaatgat actaataatg
     2761 ataattacat tgccaaatac aatcagaagg ttataaatca gatggtacgt gcgtatcaat
     2821 atctatcaca agctggtctt gataatcctt ggaaccctca tttgacattt tatagatatg
     2881 taaattattc tggtttgtca tgggacatca tttcttctcc tgtaacagag catgatagaa
     2941 aaatttatga tgaatatagg aataaattaa ctaatagcga gggcctgtat aattatttta
     3001 tagatcctaa tactggttca ataataggtt tgcctcactt aattgtgacg ttacaatcac
     3061 agatgtttct agccgacaca atcaatgata gtgtttctga ctttgcaggt tggttaggag
     3121 atttaatgac ttgctggggt gaagttaaga aattaggaat acaaatggag caaggtgtat
     3181 ttgcattagt aggtacggct gcaggtggaa catttagtct agaagattta taccaagatg
     3241 ttgacgcggt aaatgtttat accttagcaa aaagaaattc tgattcaaca gctttatcgg
     3301 ctttaaaaac gtactattgt gataagggtg ctattgatag atttaatcaa tttgttaaga
     3361 acagatttgg ttcttcagat aaaatcgctt cagtaacaaa tagtttatta gaaaatgacg
     3421 aaacagttga tatcttaggt gtagggataa tttcattttt cattgataaa atagttaata
     3481 attcacttgg gacagatttt acttatcttg attttattgg agatgagggg aaagttgcaa
     3541 atggattttc tgaaaaaatt attcaatttc aagaaaatag aagt
//
LOCUS       DADVXO010000181         3558 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.187, whole genome shotgun sequence.
ACCESSION   DADVXO010000181 DADVXO010000000
VERSION     DADVXO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.187"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(22..1830)
                     /gene="uidA"
                     /locus_tag="KB950_002685"
     CDS             complement(22..1830)
                     /gene="uidA"
                     /locus_tag="KB950_002685"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBB6858846.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1853..>3558)
                     /locus_tag="KB950_002686"
     CDS             complement(1853..>3558)
                     /locus_tag="KB950_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBB6858847.1"
                     /translation="IEETIDSMTLEEKIGQLFVNMGSSRDKNYLSNMVNQYHIGAVRY
                     NPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDAKYA
                     YELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQNPDKVLELSLAYMKGI
                     QESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGKVYSGLIEAGLPSIMAG
                     HIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGAM
                     KRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDDRLLDALKRILGLKAKL
                     GLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVPEKYKRI
                     LLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKIK
                     NIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIF
                     VSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGVSPIDAYCGLKDTF
                     I"
BASE COUNT         1103 a          731 c          446 g         1278 t
ORIGIN      
        1 aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg atgaccatcg
       61 tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga ttcctttctt
      121 atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat tccacacttg
      181 ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt aataagccac
      241 ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta ctgtatctgc
      301 tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc catctagctc
      361 ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc aaccataata
      421 acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg gcgttgattt
      481 tagataagta acgattgtta caggtctttt ctgcggatca agttctttag ttaattcaac
      541 taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat ttgcaataga
      601 ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga tcaccgtttt
      661 atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat tatcatctaa
      721 taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa taactacaat
      781 accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg atgttctaaa
      841 agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag cctcattcaa
      901 accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat aaaaaggttt
      961 attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa attcttctgt
     1021 ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat ataaataagc
     1081 gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct ttccttcagc
     1141 ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc cggtccattc
     1201 tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat taatagtaat
     1261 atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa tacctgcata
     1321 attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt cctgtactgt
     1381 ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta cagcaactgt
     1441 aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg gtgtaaaccc
     1501 acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat gtgttgcaga
     1561 tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca cttctttttc
     1621 ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat cattataaga
     1681 agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag caagttcttc
     1741 ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt caattacttg
     1801 cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa tattaaataa
     1861 atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat tcagaaagac
     1921 ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa gtattaatat
     1981 aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat acaaaaattg
     2041 ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct ggccagacaa
     2101 ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca tattttttag
     2161 ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta attttttctt
     2221 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
     2281 ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca gcaactactt
     2341 caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta tatttttctg
     2401 gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg tcagccacct
     2461 ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct tgcttaggta
     2521 ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac cctaaaattc
     2581 tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag ccttccatca
     2641 tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat ccagctgcaa
     2701 tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac atatgactag
     2761 catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca gtgattaatt
     2821 cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta aaatgttttg
     2881 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
     2941 ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta ttaatactaa
     3001 aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
     3061 aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa actttatcag
     3121 ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
     3181 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca gaaactctcc
     3241 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
     3301 cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca attaatagag
     3361 gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca gcagttcctg
     3421 gattatatcg tacagctcca atatggtact gattgaccat attactcaag taatttttat
     3481 cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa gtcatagaat
     3541 caatcgtctc ttcaatcc
//
LOCUS       DADVXO010000182         3553 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.188, whole genome shotgun sequence.
ACCESSION   DADVXO010000182 DADVXO010000000
VERSION     DADVXO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.188"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            190..2859
                     /locus_tag="KB950_002687"
     CDS             190..2859
                     /locus_tag="KB950_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBB6858848.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2950..>3553)
                     /locus_tag="KB950_002688"
     CDS             complement(2950..>3553)
                     /locus_tag="KB950_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6858849.1"
                     /translation="TDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMHMVLK
                     YNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIEKRLS
                     RYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIKITKG
                     NFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
BASE COUNT         1039 a          654 c          665 g         1195 t
ORIGIN      
        1 aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta tttttgttat
       61 acgaacaaat tatgaagtta tttattctaa attttatttt tttgtgaaaa ttagactctt
      121 tttaagcaga gaaaaacaag gatatgataa actgaataca acttcataga aatttggtgg
      181 aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg ctacgcagca
      241 gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat cgggatctat
      301 cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc taattttcat
      361 gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg gaatgcttct
      421 ctaggattga attatttatc tttaatttct tactatctag gcgggatctt cacgccactg
      481 gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac tttgcttaag
      541 gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat tcctcgctgg
      601 gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc acattcggag
      661 atcatcatgt ggctggatgc atttgtttat cttccattag tgattttggg gattcatcgt
      721 gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt gtttttatcc
      781 agtttttata tgggttttat gattggcgta ttttctttct tatattttat cgcccgtcta
      841 ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac ttctttactg
      901 gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg gtcaaatggc
      961 gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct ggatctaatc
     1021 atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc gttcgtttac
     1081 gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa agtaccgttg
     1141 aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt ctatcttgtt
     1201 ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt ccgttatgcc
     1261 tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa
     1321 gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt tgcggtggca
     1381 tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt tgtattgaca
     1441 gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa aaaactttca
     1501 ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact ggtcaatacg
     1561 aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag tttatatact
     1621 gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc cgactcgttt
     1681 taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta tggttacagt
     1741 ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct ggataaatta
     1801 ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac tttgctaatg
     1861 gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt cagtaaatat
     1921 ggcttccgcc aagctggtca aagtggcgac tatacactgt atcagaatgt caatgcattg
     1981 ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt gacagataat
     2041 ttgaccagtc agacaaactt gatcaataca ttgtctggtt taaatgaaca gtattttaca
     2101 ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac aacgactggc
     2161 gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga agtccatgtg
     2221 cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcaca attagaaagt
     2281 tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt
     2341 caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac tgcaagtttt
     2401 tatggaacga aagaagtaag tttcatggag cctaaagtcg ttggtctaga tgttgtagcc
     2461 tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga
     2521 aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac tattccttat
     2581 gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa tttcaaagac
     2641 gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag ttacttgcct
     2701 gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt tattctttat
     2761 gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc aatagaagaa
     2821 aaaccgcgaa atacacacag aagaagacga agaaaataaa gaaacgatgg aatctgtgtc
     2881 actcttctat ttaaatggca aaaatttcaa tctttaaatg acaaaaacca cctgattttt
     2941 ggtggttttt tatataccta tgactaaact atctcttgct gcttcgacaa cttctacagt
     3001 gatcttatcc atttgattga ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat
     3061 aagcctaaaa tttccttttg tgatcttaat gatagttgtg atagcctcat aatcagaaaa
     3121 atcttctagc ttgatatcaa ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt
     3181 ttcgtcctta ctaaggttat caaattcatg tgcaaatcct atgcgagagt aaagctgcgg
     3241 ataacgagac aatctttttt caataccagg cataccgata agaaccattg ccagatcatt
     3301 tctatcgtaa atatctctta gctgttctaa atgctgaagc tttaaacgat caatttcatc
     3361 gacaataatt aagtcaattg cttcataggt tttaggcaca ggaggatctt cttgattgta
     3421 ttttagtacc atatgcattg ctttagtcag acctattcta ctagttattg tgttaatatc
     3481 cgaactgaca cgagacatct tttcagctgg tacagtataa aaaactttat ttacacttaa
     3541 tatttcatct gtt
//
LOCUS       DADVXO010000183         3529 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.189, whole genome shotgun sequence.
ACCESSION   DADVXO010000183 DADVXO010000000
VERSION     DADVXO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.189"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            10..1056
                     /locus_tag="KB950_002689"
     CDS             10..1056
                     /locus_tag="KB950_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6858850.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            1058..3211
                     /locus_tag="KB950_002690"
     CDS             1058..3211
                     /locus_tag="KB950_002690"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBB6858851.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         1093 a          689 c          697 g         1050 t
ORIGIN      
        1 ggtgtttata tgactcaacc attgtaccag acaatattta atgatttgca aacggctatt
       61 ttaactggtg aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc
      121 tataaagtaa gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt
      181 atttatcgcg ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt
      241 gcttctccta caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct
      301 gttggtgatt ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg
      361 atgacgacgc ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac
      421 ggattgattt attatgctct tgatacagaa caacatcttg acttactttt tgagctttca
      481 ttgaaagaat tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc
      541 gttctttccg ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga
      601 cattcaaaaa tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt
      661 taccttggtt accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat
      721 gacaaaagcg cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct
      781 tttgtttgtg aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt
      841 tatcatatcc ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt
      901 gttgacccgc cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga
      961 gatgctttgg tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt
     1021 tcattcatca aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac
     1081 tagacatgca acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg
     1141 cgttcctttt gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg
     1201 gtttcatcct gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg
     1261 gatgggtgat ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac
     1321 ccttttccat gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct
     1381 gtcgatctgt caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg
     1441 aatcttatct attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg
     1501 ccatcagctc tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg
     1561 ttcagaagat cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat
     1621 tgatgagtta gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac
     1681 aaaaaaacaa actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa
     1741 cctggaacta gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg
     1801 gcagtcctat tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt
     1861 ttatcataat ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag
     1921 tgggcaaaaa atccactatg atacgactag tcgatctata aaacctggtg ttctctatac
     1981 aaataatggg ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt
     2041 agaaaaatac gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt
     2101 gccaaaatgg ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt
     2161 cattgcagat gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc
     2221 aatggaaaaa ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga
     2281 agattacttg acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt
     2341 agactatgct tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga
     2401 aatgacgaag aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc
     2461 aggatttatg cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg
     2521 ttggggacaa gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga
     2581 tttttcagga ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga
     2641 gctgtgcaat cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat
     2701 gagtgagatg gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca
     2761 ctatccttac ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca
     2821 gttgatgact cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg
     2881 aagtatggcc gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac
     2941 cggtgaatat gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg
     3001 aaaggaattg acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat
     3061 caaacggatg gacagagaat atacgaaatt atattttacc catcaagatt tgctgaaagg
     3121 cggcacgatc tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga
     3181 tttgcctttt tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat
     3241 caagtaataa gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta
     3301 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg
     3361 acctgagtat tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc
     3421 taataccgag ggtcagctga ttgaacacca tttagtggag aggtcgtgaa aaagctgttt
     3481 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacattttt
//
LOCUS       DADVXO010000184         3485 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.190, whole genome shotgun sequence.
ACCESSION   DADVXO010000184 DADVXO010000000
VERSION     DADVXO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.190"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(104..781)
                     /gene="nth"
                     /locus_tag="KB950_002691"
     CDS             complement(104..781)
                     /gene="nth"
                     /locus_tag="KB950_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBB6858852.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(788..1486)
                     /locus_tag="KB950_002692"
     CDS             complement(788..1486)
                     /locus_tag="KB950_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBB6858853.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1754..2938
                     /locus_tag="KB950_002693"
     CDS             1754..2938
                     /locus_tag="KB950_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBB6858854.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2944..3387)
                     /locus_tag="KB950_002694"
     CDS             complement(2944..3387)
                     /locus_tag="KB950_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6858855.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          891 a          703 c          590 g         1301 t
ORIGIN      
        1 agtatgtaca tttacttttt catgcaaaaa agataaacga aacagctttt ctccataata
       61 acaagataat tcatacattt atttgcaatt aaagaaacca cgtctacagg cgtggtttct
      121 tttttagcgc cttttctttt agacgcatcc gatttttccc atcttgacat attgatagca
      181 aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg atatctacca aaaaaaatca
      241 gtgtatgatg tgtctttacc cataattctt gtggtacttt ccgcatcagt gtttcttcta
      301 cttccatgac cgtagcatct agtttgcaga tcctcaggcg ttttgagaca cgttccacgt
      361 gggtatctac tgcgattgct ggtatcccga atgcatcccc caatacgaca tttgctgtct
      421 ttctgcctac accaggaaga gacatcaatt cttctcttga cgttggcact tgcccatcga
      481 aacgttcaat cagctgctgt gcacacgctt tgatgttttt tgctttattt cgatacaaac
      541 caatcgtttt tattttaaga atgatttcat cgattgaagc ttctgctaac gcatcgggtg
      601 tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac tgatacatct gtcgcctggg
      661 cacttaagat cactgcaatc aataactcaa atggattatt gtgcttgagt tctccatgtg
      721 cttcgggaaa cattccatac atcgtttcta gagcttccat tgttttttgt ttgcttaaca
      781 tattccgcta ctcctccaac caatttttca gtgagacttt cggcaattct tcttgttgag
      841 ctgactgttc tacttctctt tgaagaagct gctgttttct tcgtttttgg tcttcttcca
      901 cttgttgttt tgtacgtaga ttttttcttt cccacgatag caaaatgcgg tcaacgtaat
      961 taaaactata agcctgattt aatacggctt cccgcagtgc tagcttcaaa atttccggct
     1021 gataatggtc ttcttctagc cattgtccga tccgctggaa ctcgatcgaa gaaagcgggc
     1081 gtccaaattc ttgttcaaat aattgataca ctgactggat ttctttttct tgtgtttttt
     1141 cttcgtattt tttttcttgt gtttttaaat actcacctaa tagatcatag atggggtaaa
     1201 gattatatcg atctgctttt ttaccgttga caatcgtcgt ttctatcttg ataaatccat
     1261 ttgttacgag acgatccagt atttgaaaaa tctgatcttg tttgattccc atatccatag
     1321 caatcatctg caaatcaggg aaagtatctc cttcaagctg tgccatatgc aactgtagaa
     1381 caaataaaaa ttcttcagat tgtagaccga tcctatgata atttttaagt aaaagattag
     1441 atatgactgt ttgtccagcg cttaagtaat tttctaaatt caacatgatt tctcctccgc
     1501 tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa caggtttctt ttctatttac
     1561 ttcactgatc ggtcagattt acaatgtcgt ctacttgatt tttgtttgtt catgtttttg
     1621 atgcgtatct tcacttttcg gaataaataa attaagtaga tttcgctgtt ccttagaatg
     1681 ttgacaagga aacaaaatta ctatacacta tttttgtttc caagttaaca ttattgcata
     1741 aaggagtttt tacatgtaca gtttcacaaa taaacattct aaagtttttg gatacgtctc
     1801 tgtcttattc actggattag gatttactat cgtcagtcct gtacttccgt ttttaacgct
     1861 gccttattct cactccatac atcagcaagc tttctatatc actttgctca tgtctgttta
     1921 tgctctagct gccttcttat ctgcccccat tctcggtagc ttaagtgatc attttgggcg
     1981 tcatccgatc cttattatca gtttattagg atcaagtatc ggatatttgc tttttggttt
     2041 aggaaattcg atatggatgt tgttccttgg tcgtatcatt gaaggattga cggcaggaga
     2101 aatctccacc ttatatgctt actttgccga catcacggaa cctaatgaaa gaaccaaagt
     2161 ttttggatgg atgggtgctt tagtcggtat aggaacaacg ctgggtccta ttataggggg
     2221 tctattagct gagttgggga attctgttcc tatatttatt ggagcactct ttactttctt
     2281 aaatgccgtt tatggctaca catttatgct agaaagcctt ccaataaaaa aacgttcagt
     2341 agatttaagt ttctcccatg taagaccttt tcaccaatta caacaattat tcaagatttc
     2401 atccgttatt cctttattga cagcaggttt tgctgtttgg ttggcagctg gctctcttca
     2461 atctatcttt tctcaatttt cgattgacgc ctttcagtgg aaggctgggt tggttggttt
     2521 aagcttctct ttgattggaa tcttggatac tgtctcgcaa ttgttcatta tgcctcgatt
     2581 gctcaaaaaa ttttcagaac agcagatcac aagaataggg atgttcagtg aaatcctcgc
     2641 ctatctattc atcacattat ctggaattct tctactgccg attttgtttt tatttgggat
     2701 tatctgttat ggtttcggtg attctatttt tactcctgta ttcaatggcc aactatccaa
     2761 ttctgtctca gataaccagc aaggtcttgt aatgggcggt acccaaagta tacaatctct
     2821 atcgcgcgtc atcggcccgc taatcgcagg acagctttat gctgcagcac catttcttcc
     2881 gacggtattc ggatttttgc ttatgatttt tgcctattat cactacaata aaaaatagta
     2941 gcttcatttg ctacttcgta taaattctct gtatcgaccg ataaaacgaa atactgtgtc
     3001 aaattcttct tttgacatat tttcaaatac agtagcatct cgctgtaccc atttttgatg
     3061 gagttgctcg tgtctattcc ttattttttg tccgattggc gtcaattgaa aatagatttc
     3121 ttttttattc tcttcgcttt gataagtgtt tatgatccct ctagctatca gtttttttgt
     3181 tagcttactg atggctcctc tagtcatgta actagcattc gctaattttg tcacgttagg
     3241 atcagggatt ttcccgatat gttcaatcac ttctagttca tttagagtaa atcctgcaaa
     3301 ttctttttcc atttctggtt tattacgatg aataatctca ttagctaaat ccagatattc
     3361 ttctattagc agttgtcttt tatccatttc tacaccttct ttttctttca ttatattgtt
     3421 tttttgtttc ctattcaaca aagataaaaa tattttgtag gaaaacccaa tctgaaacca
     3481 agaaa
//
LOCUS       DADVXO010000185         3473 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.191, whole genome shotgun sequence.
ACCESSION   DADVXO010000185 DADVXO010000000
VERSION     DADVXO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.191"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..2232)
                     /locus_tag="KB950_002695"
     CDS             complement(<1..2232)
                     /locus_tag="KB950_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858856.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="KB950_002696"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="KB950_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          974 a          769 c          549 g         1181 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt cta
//
LOCUS       DADVXO010000186         3452 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.192, whole genome shotgun sequence.
ACCESSION   DADVXO010000186 DADVXO010000000
VERSION     DADVXO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.192"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            9..899
                     /locus_tag="KB950_002697"
     CDS             9..899
                     /locus_tag="KB950_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBB6858857.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(972..1796)
                     /gene="nadE"
                     /locus_tag="KB950_002698"
     CDS             complement(972..1796)
                     /gene="nadE"
                     /locus_tag="KB950_002698"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBB6858858.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1808..3280)
                     /locus_tag="KB950_002699"
     CDS             complement(1808..3280)
                     /locus_tag="KB950_002699"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBB6858859.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT          976 a          727 c          618 g         1131 t
ORIGIN      
        1 tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta
       61 gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt
      121 tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg
      181 aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg
      241 ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat
      301 cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga
      361 tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg
      421 atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa
      481 atacagagga tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt
      541 tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa
      601 catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc
      661 cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga
      721 atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca
      781 cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat
      841 ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat
      901 atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt
      961 ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt
     1021 tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct
     1081 tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt
     1141 ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc
     1201 aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg
     1261 gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc
     1321 cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt
     1381 gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt
     1441 acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag
     1501 tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg
     1561 ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc
     1621 aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga
     1681 aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt
     1741 ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat
     1801 tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag
     1861 ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt
     1921 ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt
     1981 ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc
     2041 gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga
     2101 tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc
     2161 catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg
     2221 cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta
     2281 gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac
     2341 cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt
     2401 cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac
     2461 gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt
     2521 cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag
     2581 tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat
     2641 catttccata agattggact aacgaatgag catgggtccc gctagctggt atcccaaata
     2701 tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc
     2761 cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt
     2821 ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca
     2881 tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta
     2941 atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt
     3001 caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag
     3061 caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg
     3121 caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat
     3181 gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg
     3241 tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc
     3301 aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt
     3361 ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta
     3421 aaactatcct ttcttattgg tatttaacct aa
//
LOCUS       DADVXO010000187         3369 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.193, whole genome shotgun sequence.
ACCESSION   DADVXO010000187 DADVXO010000000
VERSION     DADVXO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.193"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..767
                     /locus_tag="KB950_002700"
     CDS             <1..767
                     /locus_tag="KB950_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6858860.1"
                     /translation="KKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQKIGFSG
                     FSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQ
                     SKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLS
                     GKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQ
                     LYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            complement(1450..1548)
                     /locus_tag="KB950_002701"
                     /pseudo
     CDS             complement(1450..1548)
                     /locus_tag="KB950_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1813..2268)
                     /gene="tnpA"
                     /locus_tag="KB950_002702"
     CDS             complement(1813..2268)
                     /gene="tnpA"
                     /locus_tag="KB950_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBB6858861.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2511..3369
                     /locus_tag="KB950_002703"
                     /pseudo
     CDS             2511..3369
                     /locus_tag="KB950_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1115 a          581 c          587 g         1086 t
ORIGIN      
        1 aaaaaaaagt cgctgatttt attttaaatt ctgggaaaac tattatttat agtacgatga
       61 gtgatattaa gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa
      121 ttggtttttc tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa
      181 aaaaagaaca tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag
      241 ctctctattc cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga
      301 tcactcaatc aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg
      361 atttagaaaa tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt
      421 ttcaagccca agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat
      481 ctgggaaaac gaaagatact tatgattcat tgaaaatagc taaaaaaaat ggcgcaaaaa
      541 ttattgctat aactaactat attcattcac ctataggaaa gtctgcagac ttagttttac
      601 aaacagccat tgaagaattt cttaatggtg gatctttagc tggaaaaata tcacaacttt
      661 atatttgtga tctattagtt catggttatg aacaaaacaa taatattgat tcagttgaat
      721 taagagaaaa agttttgcgc tctattattg ataaaagtat tgagtgaact acccccactt
      781 agctaaacct aacggttttt aacgattgaa gtaggcaatt cttgggatga gagcatactt
      841 gatagcttat tttttactaa tagcggtggc tcttattgac caaatatccc tgtagttcct
      901 acggttcttg attttatcta aagatatttt tatcgtggaa gctattgatc aacttgaaag
      961 aaaaacgtgt agctcattat cactagttta tcgagtatgg atgaagcaat ggatcatcct
     1021 ctcttaaata aatttattaa ggatctgatc cttcaaattt tttccatgat cgatgagcag
     1081 gaacctactg aatctaaacg acatcgggca caaggaatta ttaagattgc taagcgaatg
     1141 ttttctataa agggtgttct aagctataca gtgttaattc aaaagattcc caatcatctt
     1201 gtttataaga acaacgttga agattaaaaa agtgtttcaa tgctaaaatt gcaaaatact
     1261 tcaatgttat gagacaaact gtctataaga atcaatttcc acccatttca tcacttgtta
     1321 aaagtttatg atccttttga tttttatatt aatttatctg tatttaatac agattttatt
     1381 tattagttca tctgcctatt gtattttatt ttgttgcctt accatctgaa gtaaatatac
     1441 tcttaattgt caaattaagt tagacagatc atcaatactt acgtaactca aaaatacttc
     1501 caaaggtgtt cgataatcca gtgattttct aggaagatta tttctgcgcc agcattttct
     1561 aatgtttgaa tttgtgtttt gtaatcttgg ttgcttgtgc taattcttga atagccgtaa
     1621 attgccaaaa tttcatcacc ctttctggac ataagtttct gacaccagtt aaaatcaagt
     1681 ctatcagtaa cccatacacc cgtgaggacg ggtgtatttt taatccccct agaaggggtc
     1741 gacactcgct gccccttaaa aggggatttt taaaagaccg ccaactgctc atcttcactc
     1801 acttacttct ctttattttt taaatggttc tacgtactct cttttactta taatatatcg
     1861 aatgcgatca tcagcttctt gttcacggat gtatttttca tttgtctttt gattcaatcc
     1921 gactgtactc acatagtatc tcttcgacca aaaggatttg tttccgtatt tatatttgag
     1981 atttgcgtgc tgctcgtgga ttagtaccgc actacgacct ttcagatacc ccataaaact
     2041 ggatatacat atttttggtg gtactcgaac aagcatgtga atatgatcta gcattgcatg
     2101 tgcttctatg atttccacat ctttcatttc gcacaacttc ctaagtattt ttccaagatc
     2161 ttttcgtaat tgcccataga ttacttttcg ttgatatttt ggtgtaaaaa taatatgata
     2221 cttacaattc caacgagtat atgctaaact tttatcgtca tttgacatga aaaaactcct
     2281 ttcgatctga tgagcggttg gtagccactc actagtatat cgaaaggagt ttttatcgct
     2341 aaagatactt cttccacgcg tagaaacgcg cggttttctt gtttcgcacg ctttacgcac
     2401 aaaactgact gaagtcatta agaaaataaa agggtctaaa acttttaagt tttgtaccga
     2461 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggctaagt
     2521 atacttttga acttaaatta aagatcgttc atgattactt ggatggtaaa ggcggaagtg
     2581 attatctggc aaagaaatat tccattaagg ctccatcaca agtgaagcgc tggatcaatg
     2641 cctatcaaga atttggtgaa gagggtctag taagaaagcg acaaaagaaa aaatattctg
     2701 ttcaatttaa gctggatgcg atagagttat atctaacaag tgagctgtcc tatcgtgaag
     2761 ttgccaacac acttaacatg aacaacccta atttgatcgc tagttggatg cgtcaattcc
     2821 gtgagggtgg gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc ttgtctaaga
     2881 aaaatgaacc caaaaataag aaaaaatcta caactaaagc aacgtctaaa gaacgtgaac
     2941 gcatcgagga gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc tttttaaaag
     3001 aattgaggaa gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa tcgcacgaat
     3061 catcgcaagc ctccgaggac cgtttaaatt agttgagctt ctagaaactt taaagttccc
     3121 taaggcaatc tttatgtact ggcaaaaacg attggaccgt aaaaatccag atcaagagat
     3181 tgaagcggaa atgcttaaag ttcgggagaa gcacaaggat tatggctgtt tacggatgac
     3241 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcctgatcaa
     3301 gaagctagga ataaaagtaa cgacatacac acgtaaatca cggcgctaca attcttatcg
     3361 aggacaagt
//
LOCUS       DADVXO010000188         3345 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.194, whole genome shotgun sequence.
ACCESSION   DADVXO010000188 DADVXO010000000
VERSION     DADVXO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.194"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..565
                     /locus_tag="KB950_002704"
     CDS             <1..565
                     /locus_tag="KB950_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858862.1"
                     /translation="TLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTS
                     QNISKTTLVMEEEDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKI
                     IEYLSENTQYLIAECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEP
                     IDQFKKAFEIKELPEITMHNWLKGEES"
     gene            562..780
                     /locus_tag="KB950_002705"
     CDS             562..780
                     /locus_tag="KB950_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858863.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            767..>3345
                     /locus_tag="KB950_002706"
     CDS             767..>3345
                     /locus_tag="KB950_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858864.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFR"
BASE COUNT         1258 a          442 c          692 g          953 t
ORIGIN      
        1 tactttggat tgcgaaaaaa aggagtatat gccgaaaatt cgtcagaggg gtatcgaaaa
       61 aaagagatat gtctctttaa aaaaaggtag aggtatgtac aaatttgtat atacgtatac
      121 aaagactact tcacagaaca tatcaaagac tactctcgtt atggaagaag aggatattaa
      181 tataaagagg atttacagta agattagtgt tctttatgac gcaaataacg aagatggtcg
      241 aaaattattt caagtgtatc actatttgag aaacgctaat gtagatacag aagatatcag
      301 aaaaatcatt gaatatttat ctgaaaatac tcagtatctt attgctgagt gtattataaa
      361 acaacatgaa gcatgctttt ttcaaagaaa aactgaagaa gggttatacg attatgtaac
      421 atattttttg aatggacttc agaaaagata taaaagtcaa tttttagaaa atgtagagcc
      481 tatagatcag ttcaaaaaag ctttcgaaat caaagagtta cctgaaatta ctatgcataa
      541 ttggttgaaa ggagaagaat catgattaaa aatcgcttaa tagatcaaat gtggtgtctt
      601 gatcaaaata cagatactct cgaattaatc gagagtatct gcttcaacac agtagttctc
      661 gatttaagag ctgaaaatga caattgtgta actcatatca taattaatca aaaaatggct
      721 cagcagctct ctgagagcct aagaaaatgg gctgaaggag aaaactatga aagcaattaa
      781 agaaaaaatc gttagtagaa aaataaactg gttagcactc agtgtacttg ttttaatgat
      841 tagcgttgtt tttacaagta atattgttga agctagcatt ttcaattggt ttgatgacac
      901 aaaaagtcaa acagagtttt tgaataattc tctttataga ccttacctaa aaaaatacaa
      961 tggagatttc cttagtcagt tcgggatttg gcttggatgg gctgtggtaa aaggcatgtt
     1021 tactgtgact gattcgattc aaaatatgat tccagatgtg ttggatttgt tcaatttcat
     1081 tgaaagtaca gggctaaata atgtttatca atcagtcata aatacaattg tcgttggatt
     1141 aatgatcctt tcattgatgt ttgttggcta caagatgatt actggaaaag gaacaataga
     1201 tttaagatct gttggaatga atatagtgat gtctgtagcg ttaattcttt taatgccaac
     1261 aatgatttct agcggtattc aatttgccaa aatattttac aatgatgcta ctacgattac
     1321 aaattcagat gacggagtcg cttggtcatt gataaaacaa ggagtaacag atctggcata
     1381 tattaataaa acagatcaat actcaagcat tgataaaacg gaggatcgaa ataagttaac
     1441 acgaaaaaat tttcaacaga cagatttaac acaagtgttg acggataaag tgattgataa
     1501 attggaaaaa gaaaatccag ctgcggataa tcttcgctat gaattagttg aaaattctaa
     1561 taatgaattt gttgccacaa agttttctga taacttctta tcaacatttt ctgatagctt
     1621 gaaatcaggt tattatcgat atcaagcaaa tctttggggg atttcgattg ggttgacagc
     1681 gttggcaatc gcctatgtgt tcagcgcgtt tgtgatcatt acagcaatat tagaacttgc
     1741 atttaaacgt gtgttaggtg ttcttgtttt tgcgacagat attgaaacag gacaacgttc
     1801 aaaagttgtt ttatccgata ttttacaatg ttacttaaca gttggatttc aagggtttgg
     1861 actatctatg tttgctatgt ttatcaattt tttaaatgct ggacaaggta tctccacaaa
     1921 tatttttata aaaacaattg cttatatctg tgctgtgttt gtgctgataa aaggcagtgg
     1981 aacagtcatg agatactttg gtgttgatat tggactcaaa gaaggctatg ggcaacttgc
     2041 ttctgctttt ggtatgggcg caatgctgtt tagaaaagga tcaactggat ttaatcgtgc
     2101 taaaggaagc ggaaatggaa atggatcaga cagtggagaa ggagaagatc gaaaaccaga
     2161 aaagaatttc ggcgaaactc tatcaaaaaa agctggcaaa actggaagag ctttgggata
     2221 tgcccatgaa cgtggattat caggtttagc ttcagatgga gctacaatgg ctagtgaaag
     2281 agcaataaaa ccatttaaga gtatgcgtga tatggctaat gataccaaga ataagttcaa
     2341 agaaggttta gatgatggta cagtgtccgc tattaataaa aatagcaaac ctatgttagc
     2401 taaaaataaa gaagatgaaa ctggaaaata tgctgattcc atgccaacac gtctttcgga
     2461 tagaaaagat ggtgctaagg ttgaaaatgc cgatagaatt atgtctagtt ctgaacgtat
     2521 gcgtgaagca atgaaaaata atgctgaatc gaataataat cctgtaagtg caattcagca
     2581 aaaagttcaa caagatattg aagaacgtaa aaatgctatg cacggtcaag ctaagtctgc
     2641 tgaagaatta attaatcaga agcgacaaga agctaaatac acgcctgaag caatgaatcg
     2701 tgaggaaatg ttaaggaaac gtgtagaagg gcgtacagga gctaatacgg acgaaaaaga
     2761 agcgttgacc aaagaaagaa tccaagaagc taaaaactca aataccggat tagaaaaatt
     2821 ggtcaaagaa aatctacaaa attctacaag tagtcaaggt ggccgatcag tagatgtccg
     2881 agaaaatatt caaggttcat tgaatggtca tgtcggacgg aatgttgatg taagagagaa
     2941 tttacaaagc tcttcaaatg gtcaaggtac taagacaatt gatgttcgag aaaatgtaca
     3001 aaattctaca agtagtcaag ggtttaaatc aatggatgta agagagaatg tacaaaattc
     3061 tacaagtagt caagggttta aatcaatgga tgtaagagag aatgtacaaa gttctacaag
     3121 tagtcaagga ttcaaaacag tagatgttcg ggagaacgtt caaaaagata gtggagaaat
     3181 gaaagaaaaa acacaaaaaa tcaatatcgt tgaagacaga aagtcagcga aaaaatttga
     3241 ttcaaatcat gaaacagtga ttattgatag tgaaatccgt gaaaatgata agggatcaaa
     3301 accaagacga agatttacct atgaagacaa cgagttattc agaga
//
LOCUS       DADVXO010000189         3263 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.195, whole genome shotgun sequence.
ACCESSION   DADVXO010000189 DADVXO010000000
VERSION     DADVXO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.195"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            75..503
                     /locus_tag="KB950_002707"
     CDS             75..503
                     /locus_tag="KB950_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6858865.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="KB950_002708"
     CDS             490..732
                     /locus_tag="KB950_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858866.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="KB950_002709"
     CDS             complement(1032..1247)
                     /locus_tag="KB950_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858867.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="KB950_002710"
     CDS             1354..1668
                     /locus_tag="KB950_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858868.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="KB950_002711"
     CDS             1772..2950
                     /locus_tag="KB950_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6858869.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1081 a          489 c          654 g         1039 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ata
//
LOCUS       DADVXO010000190         3212 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.196, whole genome shotgun sequence.
ACCESSION   DADVXO010000190 DADVXO010000000
VERSION     DADVXO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.196"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(353..715)
                     /locus_tag="KB950_002712"
     CDS             complement(353..715)
                     /locus_tag="KB950_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBB6858870.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            938..1690
                     /locus_tag="KB950_002713"
     CDS             938..1690
                     /locus_tag="KB950_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6858871.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1830..2753)
                     /locus_tag="KB950_002714"
     CDS             complement(1830..2753)
                     /locus_tag="KB950_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6858872.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2768..3040)
                     /locus_tag="KB950_002715"
     CDS             complement(2768..3040)
                     /locus_tag="KB950_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858873.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1054 a          605 c          524 g         1029 t
ORIGIN      
        1 taagataccg atgattgaac aaatataacc aaatgtctct acaacataat caatggcaaa
       61 agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa gaaacggata
      121 taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata taccttgcgt
      181 tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt aagaaagcag
      241 ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct tgttttttat
      301 tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt tattaaatag
      361 aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt ttgaattgta
      421 ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct tctcgtgtca
      481 tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt ttcttcgtat
      541 tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt gttcgatcta
      601 acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc actactttag
      661 gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc ttcatctcat
      721 tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca tctttcctag
      781 cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct tttatcactt
      841 aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca tctcgttacg
      901 taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa taaagaaaat
      961 gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga ttggactgct
     1021 aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg
     1081 tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata tccaagaggt
     1141 cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc aaatgttgct
     1201 ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac ttgatatgta
     1261 cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt
     1321 gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa aaaaaaaggt
     1381 tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag aaatgcaaac
     1441 tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag aagatgaaag
     1501 cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct ggcctgaata
     1561 tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac ggttcactgc
     1621 atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa
     1681 aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac ctaataacaa
     1741 aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc cattctttca
     1801 caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata aggtattttc
     1861 actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca agcttccaga
     1921 tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc gcgtccaatc
     1981 accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac catatccgaa
     2041 tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg ttttcctttc
     2101 tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa aagttgatga
     2161 catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc caacgtgatg
     2221 cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat tgattgttct
     2281 cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa aactagaggt
     2341 ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt aatgatcaat
     2401 ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc tttatgatag
     2461 cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat gatcccactg
     2521 atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat gcttttcgtt
     2581 tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc tttcatgacc
     2641 gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat tacacgtgaa
     2701 atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc cactgctgct
     2761 tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga ccgcattcaa
     2821 aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc caatatatat
     2881 cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc ctattcaacc
     2941 cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca taaaaataat
     3001 tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct ccttttgata
     3061 ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc caaagaaaag
     3121 ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac attataatga
     3181 tactgtaaat atatctttat tattagtgaa tg
//
LOCUS       DADVXO010000191         3127 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.197, whole genome shotgun sequence.
ACCESSION   DADVXO010000191 DADVXO010000000
VERSION     DADVXO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.197"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            65..754
                     /locus_tag="KB950_002716"
     CDS             65..754
                     /locus_tag="KB950_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBB6858874.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            768..2270
                     /locus_tag="KB950_002717"
     CDS             768..2270
                     /locus_tag="KB950_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6858875.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2283..2759
                     /locus_tag="KB950_002718"
     CDS             2283..2759
                     /locus_tag="KB950_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6858876.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2856..2966)
                     /locus_tag="KB950_002719"
                     /pseudo
     CDS             complement(2856..2966)
                     /locus_tag="KB950_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1028 a          417 c          616 g         1066 t
ORIGIN      
        1 cggatgctat attgtttttg gagttgttat ccaaaaataa taaaaaattg gagtgtttag
       61 gataatggat aaaaagaggt ttttagatct tatttcaaaa ggtttgattg tttcctgtca
      121 agcattacct ggtgaaccat tttacaaaga ggaagaaagt ttaatgcctt attttgcttt
      181 ggctgctcaa agggctggag cagttggtat acgtgcaaat tctgttcgag atattattga
      241 aattagaaag tgtgtatcat taccaatgat tggaattatt aaaagagatt atccaccaga
      301 atctccattc attactgcca ctatgagaga agtagatgaa ttagtagaaa ccggtgttga
      361 ggtaatcgct cttgattgca ctcttagaaa tcgacatgat ggactatcta taaattcttt
      421 tattactaaa ataaaagaaa aatatcctca tcagttattt atggcagata tttctacatt
      481 tgaagaaggc aaaaatgcat ataaagcagg aatagatttt gtaggaacta ctttaagtgg
      541 atatactgag gaaagtaaaa aacaagatgg gccagatata gatttgatta aaaaattggt
      601 agaagaaggg atttgtgtaa ttgctgaggg aaggattcat actccagaac aagcaaaaga
      661 gataaaaaaa ataggagttg caggtattgt tgtgggtggt gctattactc gaccacagga
      721 aattgcatca agatttgtta aagcattaag ttagggggag aaataaaatg tttaaaaaat
      781 tttcacaatt aggacgcgct ttcatgttac caattgctat attaccagta gccggattac
      841 tattgggact gggaggagcg ttaacaaatg agagtgctat aaatgcttat ccatttttag
      901 atcaaccgtg gttgcataca attttaagta ttatgagtta tgctggaaat gcagtttttg
      961 caaacctagc actaattttt gcaattggta ttgctgtagg acttgcaaat ggagataaag
     1021 gaacagctgg tttagctggt ggagtagctt atctagtata tactgcaact attagtgggt
     1081 ttcttgaact tttttcagca aaagatgcaa cactagatac aggtgtagtg ggatcaattg
     1141 ttgttggtgg tactgttgca tttttacaca atcgttatag aaaaattgaa ttaccacaat
     1201 tcttaggttt ttttggagga tcaagattta ttccgattgt ttctagttta gctgcaatca
     1261 ttattggttc tttcttttat ttaatttggc caccaattca aaatggttta gtagttgctg
     1321 gtgaacaaat tgcccaaatg ggtagtttag gaactttttt atatggtttt ttattgcgtt
     1381 taacaggtgc tgttggacta caccacacca tttacccttt gttctggtat acatctttag
     1441 gtggtaccga aactgtagct ggatcaacaa ttgctggagc ccaaaatatt ttctttgccc
     1501 aactagctga tccaaaccac actggtctgt ttacttatgg tactagattt tttgcaggaa
     1561 gatttgcaac aatgatgttt ggattaccag cagcatgtta tgcaatgtat cgtgcaattc
     1621 ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag cggggccctt acatcatttt
     1681 taactggaat tactgaacca gttgaatata tgtttttatt tgttgctcct tggctttatg
     1741 tgattcatgc attcttagat ggattatctt tttattttgc tgatatctta aatattcgtg
     1801 tagggaattc cttttcaggt ggtttgatcg actttttatt attcggtgta cttcaaggga
     1861 atgacaaaac taattggtta aaattaattc cttttggtat tgcatgggca tttatatatt
     1921 tctttgtttt tacattcttt atcaaaaaat ttaaggtagc tattcctgga atggaagaag
     1981 aatcagaagt tcattctgaa aaagtaataa acaaaaatac atcattacat gaagagtctc
     2041 ttatcattat tgaagctctt ggtggaaaag aaaatattga gtctgttgaa gcttgtgcaa
     2101 ctcgtttgcg tgttgctgtt acgaatgggg agattattca aaaacaaaca ataaaagaca
     2161 ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca agctgttttt ggagggaaag
     2221 ctgacttatt aagtcaagaa atcaatcaaa tattaggaaa agataattag gaggttttta
     2281 atatgtttga aattttcaaa aagaaaaaac aagaaacttt ttatgcacct tgttccggaa
     2341 aaatcatttc catcacagaa gtaaatgatc ctgtcttttc acaaaaatta ctaggtgatg
     2401 gttttgccgt catacctgat ggttcaaaaa tttatgctcc attggctggt cgagtaacaa
     2461 atatatttcc tactaaacat gctattaata taaaaagcaa ctcaggcatt gattatttga
     2521 ttcatattgg aattgataca gttgaattac aaggagagcc tttttcgatt tgtgttcaag
     2581 aaaatgatga ggttactcct gaaacctcgt tggttgatgt ggattttgac aaaataataa
     2641 aatctggtaa agatacttct gttatagttg tttttccgga gaaaaatcag ttcaatgatt
     2701 taataataga taatggtcga attgctcatg ggaattcatg tggaaaaatc actttttaaa
     2761 aaagtgtata taaaaagcta taaaataagc taaataggat atttgtcaaa tgaagttggt
     2821 gaacagaaag aactgttttc tttctgttca tttttttatt ttattagtaa tgattcccca
     2881 tgccaaatca tgatccgtaa tttaaagtta taaaagcttc gaaatccatt ggcatttcgt
     2941 tggaatacct tgatatgatt attcaaatat aaaatataga gagaagagag agagggaaga
     3001 tatttataaa gggcaaagtt atgtttactc aacttaggaa atagaaatag atcaatttat
     3061 attctcttgt caatcgaata tgaaccttag cactatatac caaatatagt aaaatgctga
     3121 aagatgt
//
LOCUS       DADVXO010000192         2851 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.198, whole genome shotgun sequence.
ACCESSION   DADVXO010000192 DADVXO010000000
VERSION     DADVXO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.198"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(10..264)
                     /locus_tag="KB950_002720"
     CDS             complement(10..264)
                     /locus_tag="KB950_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858877.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(428..751)
                     /locus_tag="KB950_002721"
     CDS             complement(428..751)
                     /locus_tag="KB950_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858878.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            938..1011
                     /locus_tag="KB950_002722"
     tRNA            938..1011
                     /locus_tag="KB950_002722"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:972..974,aa:Ile,seq:cat)
     gene            complement(1396..1893)
                     /locus_tag="KB950_002723"
     CDS             complement(1396..1893)
                     /locus_tag="KB950_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6858879.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1933..2742)
                     /locus_tag="KB950_002724"
     CDS             complement(1933..2742)
                     /locus_tag="KB950_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6858880.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          947 a          503 c          477 g          924 t
ORIGIN      
        1 caatagtact caatgttttt tatttaaacg tatcaaataa agaatgacga tcaataaaga
       61 caaaaccagt cctgataatt cttctataaa aacactatcg ggttcaaagt agttgtaatt
      121 aaaaactttc cacataaagt agatagccgg aagagtcaaa atagctaaat aaaaccgttc
      181 tttgttccaa ttcataagat aaacaatact tgcgaccagt gcgaccgcag gcacccaaaa
      241 caaaactctc accaacgtaa tcatgtaacg gactcctcaa cgattcatat aataaaagat
      301 aacatgttat tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag aatagatcaa
      361 tgaaaaatga aaaagcagaa cagttttctg ctgataagca ggaactgttc cgctgaatgt
      421 gtcttatcta tcgagaaaaa atcaatggat tgatatctaa gtaattcaat acatataaaa
      481 agaggacgat cgcattcact aaaaaggaaa gaatgatgat cccatagcga atggaaacat
      541 atttaacaaa tacttttgtt tgaatacggg catatctttt tcctttatag ctgagcatca
      601 tgacaagaag caaaagggta atcacacctt gaaaaatcat tccaataaac agaattcctg
      661 aggtttgata atttaattgg agaaaataag ctgctgccgc aactaaatcg aataaaatcg
      721 caataaatgg caaaataatc actcctaaca ttgagaataa ataaaagaat aacatttttt
      781 ctaaaataag caaataaaaa ttaaagtaac aaaatagtta tgacacaagt aaaagaaata
      841 agaataacaa tattatttta cttatatttt gaaacttgtg agagtttgtc attcgtgcta
      901 tagttgaaaa gtaacaaaaa taaattttat ttatgatgta cctttagctc agttggttag
      961 agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtac ataagaggtt
     1021 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
     1081 ttttacttgg ctgttcttat tactgcagat gcaagctttt gaataccgtt taaggaagag
     1141 gttgtgaaat cagcgttttg acctgagtat tagcttaaca aacggaaaaa tagttcctca
     1201 tattttgccg tttgttaagc taataccgag ggtcagctga ttgaacacca tttaagaaag
     1261 aggtcgtgag cctgccgttt cactctgtgt cataaaaaag ccaatctact ttgatagaaa
     1321 aagcacgtag gactaaaaca gttctgcgtg ctttttttat tgtgtgttaa atatcgtaaa
     1381 tgaagataga tatttttatg attcgacgat agagacgttg aaatctttca acagctctaa
     1441 agcggattct ccgtgttttc tactggagac taacattgga tcaacaaaaa agtctgcttc
     1501 aggaaaagca ctctgcaggc ctatcagatt agagagtaca cacaagtttg tttcaatccc
     1561 gactacttcg atctcttttt gttcttcttt gctttcaaat aaagtttcct ggatttcagc
     1621 taaagtttca ggtggaatcg taaagtaata ctttttaatc actcgttcat tttcttgtgg
     1681 agaaagaaga gggatcaatt gcaaatcttc tcgttcttct tctttgtcct tcagttcaac
     1741 tggtatatcc cgtgtgaaga gaatatattc attattttgg cgtgctttag ctaatctttg
     1801 agcaatacgt tctgccaatt gatctgactc tggaatataa aattcgctgt ttggatcaag
     1861 aatatgattt tgcatatcaa taataactaa cattttcttt cctcctacat tcatatcgtc
     1921 attttattct ttttaataga agttttttaa aatataagca acaccagctt cttcattcgt
     1981 cagtgttact tggtttgcaa tttcttttac ttgtcgctgt gcattcccca tcgcaatact
     2041 caacccgctg taattcaaca tagcaatgtc gttttcttga tcgccaaaag ccatgatgtc
     2101 actcgattcg attccaaggt aatccgccag aaaaacaagt gaatgagctt tagtgatacc
     2161 tgtaggcgtg atttctaaga aatgcggatc agagaatact gattggatac catctgcttg
     2221 cagatctctt tgtagcttct gtagtttttc ttcactctta tctgttaatg ctaatttaag
     2281 atagctaaga gaaggatctt ccaacatatt taaaatttga tcaaaagaat ggttctgtaa
     2341 attgaaatgt tcataataag ggtcatcttt gactagattt gattcatcga atgtcagctg
     2401 atcatcttgg gtttctcggc tagcaatgat ggatattttc gcttcttgag catagaagaa
     2461 ggcttttctc agtgtatcgc tatctatttt tgaacgtctg agaactttcg ccatttttcc
     2521 atccttcatt tcagcaatca gtgaaccatt ggaagaaatc aaatagccac cgattcctaa
     2581 ttcggaaaaa atatctttag cagaaatgct gttccttccg gtagcgacgg ttacgattcc
     2641 gccattttca tcaaatttcc gaagtgcttg gatattttct tctggtatct ttccatttcg
     2701 atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc attttatctt cctactttct
     2761 ttttacatat ctattttcct gttaccttaa gattaaatga gaatttaaaa aaatgaaact
     2821 aacttgcttt gtcattatga attttttcaa a
//
LOCUS       DADVXO010000193         2817 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.199, whole genome shotgun sequence.
ACCESSION   DADVXO010000193 DADVXO010000000
VERSION     DADVXO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.199"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..962)
                     /locus_tag="KB950_002725"
     CDS             complement(<1..962)
                     /locus_tag="KB950_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6858881.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1261..1896)
                     /locus_tag="KB950_002726"
     CDS             complement(1261..1896)
                     /locus_tag="KB950_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858882.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="KB950_002727"
     CDS             complement(2009..2644)
                     /locus_tag="KB950_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBB6858883.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(2678..>2817)
                     /locus_tag="KB950_002728"
     CDS             complement(2678..>2817)
                     /locus_tag="KB950_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBB6858884.1"
                     /translation="SILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLG
                     L"
BASE COUNT          809 a          472 c          411 g         1125 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt
     2701 tcttaaaatc gaattcttct tgattaataa tcccttcatc taacaactct ttatactttt
     2761 taatttcatc tgcacttgaa atttgtgttt ctttggaatt attatctaat atagatt
//
LOCUS       DADVXO010000194         2770 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.201, whole genome shotgun sequence.
ACCESSION   DADVXO010000194 DADVXO010000000
VERSION     DADVXO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.201"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            111..557
                     /locus_tag="KB950_002729"
     CDS             111..557
                     /locus_tag="KB950_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6858885.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            830..2182
                     /locus_tag="KB950_002730"
     CDS             830..2182
                     /locus_tag="KB950_002730"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBB6858886.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2353..2640
                     /locus_tag="KB950_002731"
     CDS             2353..2640
                     /locus_tag="KB950_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858887.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          974 a          453 c          546 g          797 t
ORIGIN      
        1 cgtctttttt cattaaaata caataaaatt gaattttctt ttcatgttaa gtggatttta
       61 aaatgtaaaa atagtatgat aagacgagaa taacaacgtg gggtgtttac atggaaaata
      121 agttagtcga agaggttatc gaaaagttaa aaaaagagaa cattcgaatc acacctcaac
      181 ggtacgcggt tttagaatat ttgattgaac atcattccca tccaacggct gatgagattt
      241 ataaagcact tgaacatcgg tttccaaata tgagtgttgc gactgtttac aataaccttc
      301 gcttatttac ttcgattgga tttgttcaag aaatgaagta cggcgatgct tctagccgat
      361 ttgatttcag ttcaaagcgt cattatcatg taatttgtga aaactgcggt aagattgttg
      421 attttcatta cccagggctt gaagatatag aaatggctgc aagcagactg actgattttg
      481 agattaatga acatcggttg gaattgtatg gactctgtcc ggattgtaaa gagtcagcaa
      541 aaaaggcgca aatgtaaaaa cagtttctac aagtgttata cctaaaaaaa gctagctgtg
      601 taaaaaaaac gctagctttt ttattttttt gtcttttatt cacaatctta gatataaaag
      661 tgaatattat taaaataaga atcttgtaga tattgggttc ttatgcttga aaaggaatga
      721 aactacaaac aaacctttgt gaattcacaa gcattaaact gttgtatccg ttttatttct
      781 gttatagtag ttttgtcgaa ggacaaaatg ttaatcggag ggaccataaa tgaaagtagt
      841 agtaattggc tgtactcatg caggtactgc agctgtaaaa agtattttaa aaaatcatcc
      901 agaagcagaa gtgacagtat atgaaagaaa tgacaatatt tctttcttat cttgcggtat
      961 cgcactttat gtcggcggag tagtaaagga cccagcaggc ttgttctact caaatcctga
     1021 agaattatct tcattaggag caacagttaa aatggaacac gatgtaacag acgtcgatac
     1081 agaaaataaa acagtaacgg caaaagatct gaaaacagga gaaacagaaa cggtttctta
     1141 tgataaatta gtgatgacaa ctggttcatg gcctattatc ccaccgatta gtggaatcga
     1201 tgcgaaaaat gtactcctat gcaaaaacta caatcaagca aatgaaatta ttgcacaagc
     1261 gaaagaagca aaacgtgtag taattgttgg tggtggatat atcggtattg aattagtaga
     1321 agcttttgtc gaatctggaa aacaagtgac attgattgat ggattagatc gcatcttgaa
     1381 taaatatcta gataaaccat tcacagatat cttagaaaaa gaattgacag accgcggtgt
     1441 tactcttgcg ctaggcgaaa atgttactga atttatcaca gatgaagaag gaaaagtgaa
     1501 acaagtagct acaccaacag atacatttga tacagatatg gtcattttat gtgtgggctt
     1561 ccgtccaaat acaaaactga tcgaaggaaa agtagataca ctgccaaacg gggcaatcaa
     1621 agtgaatgaa tacatgcaga caagtaatcc agacattttt gcagctggag actctgcagt
     1681 agtcaactac aatccaagtg gcacgcaaaa ctatattcct ttggcaacaa atgccgttcg
     1741 tcaaggtttg cttgtgggaa ataacttgac agatcataaa atggcgtatc gtggtactca
     1801 aggaacttca ggactttacc tatttggctg gacgatcggt tcgacaggtg tgaccaaaga
     1861 aagcgcaact ttgaacgatc tagaagtcca agcgactgta tttgaagata attatcgtcc
     1921 agagttcatg ccaactactg aaaaagtaat gatggagcta gtctatgaaa aaggaacgaa
     1981 tcgtatcgta ggtgctcagt tcatgtccaa atacgacatt acacaatcag caaataccat
     2041 gtctttagct gttcaaaata aaatgactgt agaagatctc gcactttcag atttcttctt
     2101 ccaaccacat tttgaccgcc catggaatta cttgaaccta cttgcacaag cagcgctagg
     2161 cgaaatcgaa actgcacaat aaagaaaaag ggttgtgaca caattttgtc acgaccctct
     2221 gtcttttttt gtgaaaagta caattagttg tctttcttcg aattttttgt cacaataagc
     2281 agcaaagata aagctgttac ataaaagaac aagcagcttt atttgatttt gaaaagaaag
     2341 gtgagaaaga agatgaataa agaatatttt gtccaacaag atgcattttt agaagattat
     2401 aaaagtgctt cgaagaaatt aacgatcaat attgatgagt tggcagatca agtactctct
     2461 cagttgaatg aaacgcaaga ggaatattac acgatgccag caaatgacgc caaagataat
     2521 gtttctcatc gttttccttt cagtagacgc atctactcag atgaaaatga cggcacattg
     2581 aatacagagt acacatatga aggtcagcca tatgtgctag aaactgatga tgatctttaa
     2641 agacagtttt tgaaaaggcc gctcatcctt aagtgatgac aaaaaatcat cacaattacc
     2701 gatgactgtc tctttactat cttttaatca gaaaaagggg ttatgacaag ctttgtcaac
     2761 cccttttttt
//
LOCUS       DADVXO010000195         2768 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.202, whole genome shotgun sequence.
ACCESSION   DADVXO010000195 DADVXO010000000
VERSION     DADVXO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.202"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(75..1874)
                     /locus_tag="KB950_002732"
     CDS             complement(75..1874)
                     /locus_tag="KB950_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB6858888.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1998..2594)
                     /locus_tag="KB950_002733"
     CDS             complement(1998..2594)
                     /locus_tag="KB950_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6858889.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          976 a          522 c          480 g          790 t
ORIGIN      
        1 aaaacgcata tacctgcggt ggcaggttat gcgttttttg ttgtataaag acttttttca
       61 ttaacgattt ttgattagcc gactcccaaa acaaaattta gcagtttatc tgaataagtg
      121 atttcttcat ctgtttgttt cacactttca tttagtagtc ccatctcatc ggttatgata
      181 ccatccacac cataaaacat catgcgactc atagcatctg aatcgttgat tgtccataca
      241 tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat cgtactatat
      301 tccattgtca gaaaatcagc aggcgtcaca gggggcccaa caatattgaa aggtaagatg
      361 tagcccacat aaaagtttgg ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa
      421 ctttgcaaaa tatgatggtt ctctaagata actttgcggt atttatggac aaatcgttcg
      481 acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa taagtaattt ttggtttagc
      541 ttatgtgctt tttccaaata agaatcaaaa gaacaaacag gggcttgcac tccgttttca
      601 gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc atttacccct
      661 gttaagtgac gaagatttgc atcatgcatc actacgaaat gaccatcttt tgtttcctga
      721 acatccatct ctacataatc aggatgatag tctctgcttg ttttttcaag tgcttctaaa
      781 gtattttgcg ccccattttg ggtactcaca cctctgtgag agacagtcac tggttttgtc
      841 tgaacagctg aagtcagata atccatattg taaagacaga cgccaatacc gaaaaaaact
      901 gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac gcaagttggg ggcttgtggg
      961 ataaaccact tagggatctc aggcaagaag ccttcgtcat ccatgaaatc aacaatgata
     1021 taaaaaatcc ctacggtaga catcacgata ttgaataata aaatcccttg taaaagcgtc
     1081 atagcaaaca cagcgctgat caatgcatag tctgggaaaa attgttcaac catactttgt
     1141 gccaaaataa caacgatata tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca
     1201 ataaattggc ccgtgatggc caaaagacgc gatttggtca atgaccagct ttcccgaatg
     1261 gctgctcgaa aaggacgatc tcgtagaatc atttccggta aagcaaaaat cagtcgaatc
     1321 ccaatatagc caaggaaaat ataaacgagt aaaaaggaac tgacgatgat ccaacgatta
     1381 gcaaaaataa aatccataat gaaagcgggg atttttattt ttgacagtag atcagaatta
     1441 aaacttaatc cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa caaaaaagta
     1501 atgggtctca ctttcttcag ctgaagtatc gtcaagtgta agagttgctt caaagagatt
     1561 ggttctttct ttttaataaa aaaaacactc atcaaaagaa aagtaaattc gaaataaact
     1621 aacagtaaaa tcgtcatcaa aactacgatt aacgagagta gaaccccagg atgttgagaa
     1681 aaaattgaag gaatattgtc ataagacaaa tagtcaattg cccctcggtg taaaataaaa
     1741 cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa taaagccatg catcagcaag
     1801 acatcacgga aataagcttg tgttcctttt aaaaagtccc aagcattacc taagctacgt
     1861 ttcaaataat tcatcctatc acctgctctt cacattgtag cgcaaatcta atgaaaaagg
     1921 aaagggtgca gtgtgttttg cagacccttt cccagattta acattgtcaa tcaatgacta
     1981 attttacaaa ataaatatta tccaagaaga ccacttagat attgacctcc ataaatcaag
     2041 gccatactat ataagaaaat cgacagtggt ttagcaatca aaatgattag tgtatatcgt
     2101 ttcactgaca tgtttgtcaa tccagccatc aaacataacg catcgtcagg agcaactgga
     2161 agaaaaatag ctagagcaaa cagcttctca aaacggtttt gattatccag ccagccaata
     2221 tatttgttat atgtcttatc gctaatcatg gaaagtatga atggctttcc ataacgacgc
     2281 cctagcagaa aaatgataat cgaaccaatg cagattccaa tatagttata gataaatcca
     2341 gcataagggc caaatatcag gacaccagct gcagtactga tccctccagg aatgattgga
     2401 ataactactt gcaggatttg gatgaagaca aaaataatcg gtcctaaaat gatggaatca
     2461 cctactaatc ctcttaacgc attcaaatct ttgaaaacac ctaaattaat aaaataaatc
     2521 gtcagcgcga ttgataggcc taatccgata atggatatga aatttatcag ttttcgagag
     2581 gcagcaatat tcattattca tcaactcctt agaggttctt tgttaactat actttactag
     2641 aaaagcaact aactgcgcca ttttttgata tgacagaaaa caaacaattt tgtaagttca
     2701 tcataagtat aaaagattga tctcaaaaaa acatccaact aaagacttat ttaataactt
     2761 gttttttt
//
LOCUS       DADVXO010000196         2749 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.203, whole genome shotgun sequence.
ACCESSION   DADVXO010000196 DADVXO010000000
VERSION     DADVXO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.203"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            26..1504
                     /locus_tag="KB950_002734"
     CDS             26..1504
                     /locus_tag="KB950_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBB6858890.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1572..2663)
                     /locus_tag="KB950_002735"
     CDS             complement(1572..2663)
                     /locus_tag="KB950_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBB6858891.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          960 a          498 c          442 g          849 t
ORIGIN      
        1 ttagcaacaa aaaaaggagt gcatcatgaa aaagttatct caaaaacgtt tagctcaaat
       61 cattattgat tctagaaaat ctctgaaaat gacacaacaa caacttgcag tagctactgg
      121 tatcaaccgt gccttaatta gtcgattaga atctctcgat tttatcccgt caatacaaca
      181 attagaatca ttagcagaag ttctaaattt tgaaccaaca gatctattta taaatgaatc
      241 aaataaatca aacagaacaa aacatcagtc gaagaatatt gctgtagccg gtactggtta
      301 cgtaggtctt tccattgcta ctcttcttgc ccagcacaat caggtaacag cagtagacat
      361 tcttcctgaa aaagtaaaac aaattaacaa taagaaatct ccaatacaag acgattacat
      421 tgaaaaatat ctagctgaaa aaaatcttaa tttaattgca acgactgatg gtgcagccgc
      481 ctatagagaa gccgattttg ttgtcattgc tgctccaaca aattatgata gtaaacaaaa
      541 ttattttgat acttctgctg tagagtcagt aatcaaattg gtgttagaag tcaatccaaa
      601 ggcaataatg gtcatcaagt cgacggtacc cgtaggctat accgaaagta ttcgtaaaaa
      661 atacaatact aaaaatattc tattcagccc agaatttctc agagaatcta aggcattata
      721 tgacaatcta tatccttcgc gaattattgt cggcacggac caaactgaca aaactttacg
      781 agaagcctct cacacttttg cagatttact ccaagaagga gcaatcaaaa aagatatcga
      841 aactcttttc atgggattca cagaagctga agcagttaaa ttattcgcta atacttacct
      901 tgcacttcga gtatcttact tcaatgagct ggatacttat gcagaaatga aaggactaaa
      961 tacacaagaa atcattcacg gggtttgctt agatccaaga attggcacac attataataa
     1021 tccatctttt ggatatggag gctattgttt accgaaagat acaaaacaac ttctcactaa
     1081 ctataacgat gttcctcaga atatgataag tgcaattgtc gatagtaatc gtacaagaaa
     1141 agactttata gcagatcgag tgttagaact tgctggcacg tatgaggcta acagttcgtg
     1201 gaatgcagaa aaagaaaaag aggtagtcat aggtgtatac cgtcttacaa tgaagagtaa
     1261 ttcagacaac ttccgtcagt catctattca aggagttatg aagcgaatta aagcaaaggg
     1321 ggcaactatt gtaatttatg aaccaacact tgaaaataac actacatttt ttggttcaaa
     1381 agtaataaat aatttagatg attttaaaaa tatcagtcac agtattattg ctaatcgtta
     1441 cgatagtaat cttgatgatg taaaagaaaa agtatatacc agagatttat ttcaacgaga
     1501 ttaaaaaata acacctcaga tttgtagtgt ctgaggtgtt tctataaact tttataagta
     1561 aaaaaataca attatacttc ataaaaagac ttataccact gcgcaaattt acgaagcccg
     1621 tcacgcaaac ttgtacttgg tttaaattca aaatctcgtt ctaacgcact agtatctgca
     1681 taagtaactg gcacatctcc tggttgcatt ggaactagtt cggtatgaga ttcaaagtcg
     1741 taatcttctg gtagcacccc tgctgcaatt agctcttgtt gtagaatatc tacaaaatct
     1801 agtaggtttt ccggcgtatt gttaccaata ttataaatat gatatggtgg gattggtagc
     1861 ccatcttccc catcaatttt ctctggagcg cactgcataa ccctctttac tccttcaaca
     1921 atatcatcta tataggtaaa gtcacgcttg caattcccat aattgaatat ttggatatta
     1981 tcaccagcac gaagtttatc agtaaaacca aaataagcca tatctggacg cccagcagga
     2041 ccatatactg taaagaaacg taaccctgta gacggaatat tgtaaagttt agaatatgca
     2101 tgtgccattg attcattact tttcttagtt gctgcataca acgatacttg attgtctact
     2161 ttatcctctg tagaataagg aattttctta ttagtaccat aaacggaaga cgatgaggca
     2221 tacactaaat gttccacacc agattctcca ttatcatatg aatgacgaca agcttcaaga
     2281 atattataga atcctattat gttagattcg atatacatat ctggattagt aattgaataa
     2341 cggactcctg cctgagctgc taaattcacc acgatacttg gtttataatt atcaaagatg
     2401 aaatcaattg ttgctttatc agcaatagaa cctttgataa aattccaagt agatcctgga
     2461 tggttctttg cctccgtttc tatctctgat aatctatagt tcttaataga gacatcatag
     2521 taatcattca tattgtcaat gccaatgatt tttattgatg actgagtacg taataactca
     2581 agaaccaaat ttgatccaat aaaaccggct gcgccagtta ttaatattgt tctatttgat
     2641 aaggacgtgt ttaattccag cattttttag cactcacttt ctttttttct tatgaataca
     2701 tatttgttat atattaaaaa atcttattta gacttatcta ttgtcactt
//
LOCUS       DADVXO010000197         2684 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.204, whole genome shotgun sequence.
ACCESSION   DADVXO010000197 DADVXO010000000
VERSION     DADVXO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.204"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            174..518
                     /locus_tag="KB950_002736"
     CDS             174..518
                     /locus_tag="KB950_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBB6858892.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="KB950_002737"
     CDS             519..938
                     /locus_tag="KB950_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBB6858893.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="KB950_002738"
     CDS             complement(1030..1380)
                     /locus_tag="KB950_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858894.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="KB950_002739"
     CDS             complement(1346..2677)
                     /locus_tag="KB950_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBB6858895.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT          999 a          485 c          523 g          677 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acac
//
LOCUS       DADVXO010000198         2673 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.205, whole genome shotgun sequence.
ACCESSION   DADVXO010000198 DADVXO010000000
VERSION     DADVXO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.205"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            279..1064
                     /locus_tag="KB950_002740"
     CDS             279..1064
                     /locus_tag="KB950_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6858896.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1077..1928
                     /locus_tag="KB950_002741"
     CDS             1077..1928
                     /locus_tag="KB950_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBB6858897.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2052..2504)
                     /locus_tag="KB950_002742"
     CDS             complement(2052..2504)
                     /locus_tag="KB950_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858898.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT          838 a          440 c          565 g          830 t
ORIGIN      
        1 aaaggggctg tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga
       61 agtagcttct tcggaaataa gccgaaattt cttgaagctg accacttctg tcacaacctc
      121 tttctttata gaaataaaag gacactagca attcatgaaa acgattgcta gagcgtatca
      181 tttgctttag actatatatc ttggtgtaca ctcactttaa ggcggaaata aaagttatcc
      241 gtaataacag gtttaggtat gaaaaaggag tgggtactat gtacaaggaa ttagaaaaca
      301 aagtcgcagt agtaactggt ggctcaaagg ggattggaac agcgattgct caacgattcg
      361 ggaaagaaaa aatgaatgta gttgtcaact ataactcgga taaagaagga gcagaaaaag
      421 cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt
      481 ctgaagaagg aatacagcgt ttacttgata cggctttaga aaattttggg acactggacg
      541 tttgggttaa taatgctggg atggaaaatc agcatccaac ccatgaactt agccttgaag
      601 attgggaaag agtgttaaaa gtcaatttga ctggcgtttt tatcggaacg aaagcggctt
      661 taaattactt tacagagaat aataaaaaag gaaatatcat caatatgtct tctgttcatg
      721 aacagatccc atggccaacg tttgctcatt acgctgcttc aaaaggcggg gtaaaattat
      781 ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat cagaatcaat agtataggcc
      841 ctggtgctat ccgtacacct attaatgcgg caaaatttga tgatcctgaa caaaaagaac
      901 tcttggaaag tatgatccca ctaggacgcg taggtgaacc aaaagaaata gcggcgacag
      961 ctgcatggtt ggcttcagac gaatccagtt atgttacagg tattacacta tttgcagatg
     1021 gtgggatgac tttatatcct tctttccaag gtggaaaagg ataagggtga ataaaaatgg
     1081 gtttattgat tgcattgatc ccggcagttg gctgggggat ccagccactg attttgaaaa
     1141 agattggtgg cagaccaaca aatgagatat taggaactgg tattggagcg gtgcttgtcg
     1201 gcttactcgt tcaattattc gcttctccag ctggtatttc agtgactaca tttttgatta
     1261 gtttactttc tggtgcattt tgggttattg gacaaacagg acagtataca tctttcaact
     1321 tgattggtgt ttctaaaacg atgccaattt cgactgcatt acagttagtc ggaacctcgt
     1381 tgattggtgt cttcgctttt ggagaatggt ctggaagtat ggggaaaatc attggagcag
     1441 cagcaatcat tcttcttgta tttggttctg ctttaacggc aatcagtgat gatcctggaa
     1501 aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc aacaagtatt ggttattggg
     1561 tatacagtgc attgcctaaa ctagttgatg caagcggact ttccattttc tttccgcaaa
     1621 tgatcggtgt ctttttaggg gcagtgattt atgtgatttt ccgtaaacca accgcatttt
     1681 ctgaagacaa gagttggaaa gcaacgatcg ttggagccat ctttagtgtg tcagcattgg
     1741 cttatatttt ttcagcaaat gaaaatgggg ttgctactgc atatatcatt acacagctaa
     1801 acgtagttat ctctacatta ggcggtatgt acatcctgca tgaaaataag acaccaaaag
     1861 aaaaaaaatt aacgattatc ggattattgc tgatcgtagt aggaagcatg gtcacggtct
     1921 ttatttgagg ataaagatgg taaaatttca tttattaaaa aaatatttgt ttgaaccaaa
     1981 cagtaagtac ttataaaaaa atagcgctag aatttccaac atctctggaa tttctagcgc
     2041 tttttaattg tttaagatac aagggaaata tctgtctgga tcaataattt ttcattgatc
     2101 cattgactaa agtttacgac ataattgaag ttaccaccga ttagtatctc cgcatgattg
     2161 tctttataaa gatcgtattg taaaagacgg tcttttttat ttgcgtaaac atcgttatgg
     2221 tactggacta attgttctcg gaattgattg attgctgtaa aatcacgata ttgaaggttg
     2281 aaacgcatcg tgattggtgc atttagcatg aatcgctgca tttcttttgc tgcttcctct
     2341 gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag aaaatttcca ttgcccgcca
     2401 acctttgttc cttttagttt tccttcatga agataatttc tgatagaacg tgtagtaaca
     2461 cctaattgtt cggctaattc atttactgag taaaaatctt tcaatgttca ccctcctatg
     2521 tgaatggttc tactgactta gttgatattt gcttaatcac gaaatgtaga aagaacgatt
     2581 ttaattaagg tcattgtaaa aatcaagtca gaaaattcta acttttattt ctctaaagaa
     2641 attcgttctc tcctaaagtg atattatatt taa
//
LOCUS       DADVXO010000199         2672 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.206, whole genome shotgun sequence.
ACCESSION   DADVXO010000199 DADVXO010000000
VERSION     DADVXO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.206"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(51..2522)
                     /locus_tag="KB950_002743"
     CDS             complement(51..2522)
                     /locus_tag="KB950_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6858899.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
BASE COUNT          769 a          547 c          469 g          887 t
ORIGIN      
        1 aagatacacg agtggttgtt cttttttact cttttttgag atacttttcg tcaattttct
       61 cgtttaaata tcgatagaaa ttttttagct acgcgactaa acggcttttg ctctttttca
      121 tttttacgtt caaacaaata ttctaccaat gtggtcaaat gcctaaaaat cgaatctgcc
      181 gagaaaaata gaatgctcaa cagcaaaagt gatacagctg agataccggt gattgaaaac
      241 agcttatgca aaaagatgac cgaaaaaaag ttaccgctag cacagaaaag aatgatctga
      301 cttctgaatt tattcagcgg tgaacagatt ttatagagaa cgaggaaccc tactgcaatc
      361 aaaagcatgg tagatgctgt agaaacatct gttttattta gattcaaagt caccccgcag
      421 atcaccaacg caccgactac gatcatatct gttagtccac ctggcacagc tttttccatc
      481 acattcatga tgaatttccc tctgattcgc tttttgtttt cttctagagc aagcaagaaa
      541 gatggaagtc cgatagtaaa caaactaatc aatgtgattt gtgatggttc taaaggatag
      601 gtcagagcaa agatcactga aaacagtgag agcaaaaagg agaaaatatt cttcactaaa
      661 aacaagctgg ctgatcgctc aatattgttc acaacgcgcc gtccttctgc aacaacttct
      721 ggcatgcgtg aaaagtcaga atcaagtaag accacttgtg aggcctgcat ggcggcttca
      781 tttccggaag ccatcgctat actgcagtct gcttctttca tggctagaat atcattcacg
      841 ccatcacctg tcatagctac tgtattgtta cgctttatca attgaacgaa gattttcttt
      901 tgttctggct tgacgcgtcc aaaaactgta tatttttcga cagctgcttc atattcttct
      961 tctgataatt tagatgcatc aacgtactga tcagcatgtg aaatccctgc ttggacagct
     1021 acattggata cggtgagcgg attatctcca gaaatcactt tgatggctac ttgctgtttt
     1081 ttgaagtaat caaatgtggc tcttgcttct tttctgatag gattagcaat cagaatatag
     1141 cctaatggtg tgactgcttc tgtcaatact ggttcttcaa gaatcccatc gtacacacca
     1201 aagaccaaca cgcgatagcc tttttcagcg aaagaaacaa actcttccgc atattccggc
     1261 aattgatcgc gcaaaaccat ttcgggcgct cctaaaacat acaccttgtc tgccaacgct
     1321 acactgctga atttttggac agaagaaaaa ggaataacat ggtgatagga ttgatcagtg
     1381 gtttttgtaa aatatgcttt aacagcttcc attgttgcat tgtctcctgc catcgctttt
     1441 gcatagtcag caatgcatgc ttccaattga tctgactgac tgattatttc ttgtttttta
     1501 gagacgatca ttttttgaac agacatttta ttttctgtga tcgttcctgt tttatctaca
     1561 caaaggacat ttacccttgc caatgtctca atgcttttca tattgtgtaa aagaactctt
     1621 tgctttgcta gtttagtcgc acttaatgct aatgcaatcg tcgtcagcaa gtacagtcct
     1681 tcaggtatca taccgatcaa tgctgcttcc attgcaacga cactttcttt gatcgtgttt
     1741 ccattaaaaa agtaactttg agaaaaaagc aggataccga taggaataat gatgatccca
     1801 atccatttga tcaattgatt aagagaagca atcatctccg actgttctcc ttctcccatt
     1861 gtcttggctt tgactgtcag ctgggaaata tacgaatctg ctccaacttt gtctaatcgg
     1921 gcgtaagctt ttccggaaac aatgaaactg ccggacatta atggatcacc tatttctttg
     1981 gttatttcat ctgcttcgcc tgtcaataaa gcctcattaa cttgcagttt cccttccacg
     2041 acctctgcat ctgctggaat ttgatatcca gtctctaaaa taacaatgtc atctattaca
     2101 aggtcttcta tatctattgt ttgttctatc ccgtctcgta tgacattgac ttttgctgta
     2161 tttaaaacat ttagttcgtc caagatcttt ttggatcgaa tttcctgaaa aatggcgatt
     2221 cctgtattgg ctatgataac tggtaaaaaa gtcaagttct tataggattc tactaaacaa
     2281 agcaaaatgg ctaagataag aaagatcaaa ttgaaatacg taaatatgtt ttccaatacg
     2341 atttgctgat ttgttttgaa tgtgggatcg actgaattat ttgctgcacc tttttctatt
     2401 cgttccttga cttgatctgc cgttaatcct tctttatacg atggaaaaag acggatgact
     2461 ttatctgatg atctttgtgt tactttcttt tttttacttc gatttatcca tttattcttc
     2521 atgtggctac ctcgattgat ttcgttttct ggatatttcc aaacttctcc tatccagtat
     2581 agaacagata tggaaagtgt tcctcacatt aagcttgcaa acacctttca ttcctgtcat
     2641 atagcaaaaa aaaagggttc tataatattt tg
//
LOCUS       DADVXO010000200         2636 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.208, whole genome shotgun sequence.
ACCESSION   DADVXO010000200 DADVXO010000000
VERSION     DADVXO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.208"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(891..1409)
                     /locus_tag="KB950_002744"
     CDS             complement(891..1409)
                     /locus_tag="KB950_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBB6858900.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2527..>2636
                     /locus_tag="KB950_002745"
     CDS             2527..>2636
                     /locus_tag="KB950_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6858901.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          871 a          478 c          389 g          898 t
ORIGIN      
        1 actaaagtta tttgttcttt tattaaatac acctacaact tataaattga tggtatttag
       61 ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat tgctactcaa
      121 tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt
      181 tacatcttgc agaaatcgtt actgggatta aactaagcaa taaaaactaa tgttcccctc
      241 attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt tcaccttcag
      301 gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta ataggatagg
      361 cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt atcgtatgtt
      421 ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt tgaatcgttc
      481 ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg acttgcctta
      541 cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac tatcggcggt
      601 ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa gtccctttgt
      661 agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat ctgttggcca
      721 accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga gtgcacttgc
      781 attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg ttcttactgt
      841 taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat ttacaaagct
      901 aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac tgtatttaca
      961 tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtctgc ccattttctt
     1021 ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt aatatcatca
     1081 atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata ggtatttgta
     1141 aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg cgttttaaca
     1201 gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca accaacttga
     1261 tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc cttcaattct
     1321 tcaggaatag tttcaaattc taaaatatat ttagtataag tcctatttat ttcttttttt
     1381 aattcttctt tatttttata tgttctcatt atattacctc cataaattcc taattgtctc
     1441 ctttaattca tctataattt catccacgat attttaatcc gttctacaat gcatatctga
     1501 tatgcattga ctgtctaact catatgtgaa tgatttataa aatatcccaa cgtctcatct
     1561 aaagaaatgt tgatcgtcag caaggagcta aaaaatacga atatatttct tttgtagtaa
     1621 aatcggactg gtttgcatat cattttcttc gtaaatatca aaatcatgtt aactactatc
     1681 aattactaat aaaaactacc actttaagcg ttaagaactg ttaagtttag ttaagtgagg
     1741 atagttcaca aaatccattg ttaaacttca cgaaagtggt aaaactcaaa atgctttata
     1801 taggattaca gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa
     1861 aaattgacga tgacacgttc cttacggctc atcagatgaa acagctacaa aaacgaaatg
     1921 atcaactcga agaaaattta attctaaaaa agcgattgcc atattcacgc ctcgctcgac
     1981 gaactattaa aggtcaagat ttacctgaag atcatccttt ttaggaaatt gaagcttttt
     2041 atgttgcaca atgcatttat aatactacaa tgatgttttc accagatatt cttctagggt
     2101 gcggtgtaat gaaacagata cacttgcaaa agaaaattca ccgcgaattt actaaaatta
     2161 tgaataatta tgttaatctt ccgaaatttt tttccaaaat taagggatca agctggtgtt
     2221 atagattgtt taaggttggc aaaaaaataa cttcttttta aacaattata gaaaaccgat
     2281 actctgttaa gttggttgac acggtgtcga tttttatgac tttcttattc tgaaacttca
     2341 ctaacggaca gtacctaaaa agtctatagt atggacgttt acgagtacta ttttatcaaa
     2401 aatgctgctc atttcaactt atatgtttta gtgaactacc cccacttagc ttggataggc
     2461 tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat accaggagga
     2521 atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca acaaatcgtc
     2581 aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga actaac
//
LOCUS       DADVXO010000201         2581 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.209, whole genome shotgun sequence.
ACCESSION   DADVXO010000201 DADVXO010000000
VERSION     DADVXO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.209"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            23..1291
                     /locus_tag="KB950_002746"
     CDS             23..1291
                     /locus_tag="KB950_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBB6858902.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
     gene            1455..>2581
                     /locus_tag="KB950_002747"
     CDS             1455..>2581
                     /locus_tag="KB950_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBB6858903.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLT"
BASE COUNT          738 a          482 c          561 g          800 t
ORIGIN      
        1 ttttattgag gaggaatttt ttatggaaaa acaattaccg agtccagtac gcattttagt
       61 gtacatgctg aagtggctgg tgatcacagc gattctcggt atattcatgg ggagcttatc
      121 tgcttttttc ttaaatagcc tgacagttgt gacagatatc cgactatcac atccttggct
      181 cctttatcta ctaccagtta gtggtgcgct ttttgcttat ctctatgcgt atcacggcgg
      241 tctttctagt cgtggaaaca acttagtgat cgatcaagga aatggtggag aagaaaaaat
      301 cccgttgcga cttattccac ttacactgtt tggaaccata actacccatt tatttggagg
      361 atctgttggc cgtgaaggaa cagcagtcca aatgggggga gcgttagcag ataatacagc
      421 acgtttgttc cgattggata aatcagaacg ggaaatcttg attatcagtg gaatcagtgc
      481 gggttttagt tctgtatttg gcacaccttt agctggaaca ttatttggat tagaagtgtt
      541 ggcaatcggg aaagtccgaa cagaagcgat cttccctagc ttctttgctg cactgtttgc
      601 aaattttatg acagaaagct atggtgtttc tcatctacat taccaaatgg gtgtgattcc
      661 tgaatggagt atgttgctgt tcttcaaagt ttttgttgct gggattgctt ttggtttgat
      721 tggttgggta ttcagccgtt cgatcgtctt tttaaaagcg cgatatgcac aatggattcc
      781 tcatcctgtt tggcgaaata cgataggtgg ggctgtagtt gtagccgcag cattgatttt
      841 gcaaacacaa cgttatctcg gattgagtct tccattactg caggaatcat ttaatggaca
      901 ggcacatgtc tatgatttta ttgggaagtt attcttcaca gttttgtctt taggcgcggg
      961 atatcaaggg ggagaagtga caccattgtt tgaaattggt gcaacgcttg gtgcagcact
     1021 agcgcctttg ctccatgttt ctgttccttt tcttgcagga ttgggcttta tcggagtatt
     1081 ttctggtgcg acaaatacac cgattgcctg ctttgtgatg gggatcgaat tgttcggttc
     1141 acaagcggca gtatacttct ttatgatctg cttgatcagc tttatgtgtt caggcaacag
     1201 cggtatttat tctgctcagc aagtagcggt cagaaaaggg atactctttt tgccaatagt
     1261 taagagaatg aaagaaaaaa acaaggtgtg acatatgttt tgtcacacct tgttttttgg
     1321 ctctataatt tatgggactg atgaatcaga attgattctt cagccccatt ttcattttag
     1381 gtattaaaaa acatatcgtt ctctagtatg attaaatcac cacaaaataa acaactgaga
     1441 ggacgatacg ctatatgaat gattctatca aaaaaatgct gagaataaca gagaaagatt
     1501 tgatgattac agaggtctct tacgagaccc ttcagaagaa aaagacgttg gtcgtcgatg
     1561 ctgttctctc gcctactcct cgtgcttgta gaagttgtgg ttctactgtg gtagatggaa
     1621 acgggaaagc aattatagtg aaaaatggaa aaaaggaaac gattgtccgt tttgaacaat
     1681 acaatcatat gcctttggtt atgcgcctaa aaaagcaacg ctatacctgt aaaaactgtc
     1741 gcacccattg gacggctcaa agttattttg tccaacccag acattcaatc gcaaatcatg
     1801 ttagatataa aattgcttct ttactgactg aaaaagtatc tttatctttt attgcgaaaa
     1861 gctgtcaggt atctttgacc accgttattc gtacattgaa agagtttaaa agctatttac
     1921 caaagcaatc taagaagatc ctccccagag tattgatggt tgacgaattt cgttcgcatg
     1981 cttccataga agataagatg agctttattt gcgcggatgg cgaaacagga aaattaatag
     2041 atgttttgcc tacgcgtaaa ttacctcgat taacaagcta tttcttaggt tgtaccaatc
     2101 cagaagaagt agaattcttg gtgacagaca tgaacgccgc ctacttccag ctcaccaaac
     2161 gtgttctgcc aaatgcgaaa gtcgtgattg atcggtttca tattgtcaaa cacatgaatc
     2221 aagcgttcaa tgagttgcgt atccgtgaaa tgaatgaact tcgtaaagcc ggacagaaaa
     2281 gccaggcaga aaaactgaaa aagaactggc gctttttgct aaaaaatcgt gcaaacatca
     2341 accattatga atacaaaaca tggaaaagtt tccgagcacc aaaataccca tttcttactg
     2401 aagcaatgat gattgatcga ttgcttgagt tttctccgcc cctaaaggag gcgtatccct
     2461 tttttcatga attagttgaa gcctttcgag acaaagaccc tgacttattt ttctccttat
     2521 tggcagaact tcccgaaacg ttagatgaca gctttcggga aaagcttcaa aaccttctga
     2581 c
//
LOCUS       DADVXO010000202         2579 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.210, whole genome shotgun sequence.
ACCESSION   DADVXO010000202 DADVXO010000000
VERSION     DADVXO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.210"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            512..607
                     /locus_tag="KB950_002748"
     CDS             512..607
                     /locus_tag="KB950_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBB6858904.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(909..1154)
                     /locus_tag="KB950_002749"
     CDS             complement(909..1154)
                     /locus_tag="KB950_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858905.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1499..1783
                     /locus_tag="KB950_002750"
     CDS             1499..1783
                     /locus_tag="KB950_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858906.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1786..2202
                     /locus_tag="KB950_002751"
     CDS             1786..2202
                     /locus_tag="KB950_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858907.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          852 a          475 c          425 g          827 t
ORIGIN      
        1 aaaagttgtt agtactcaga taaaaagttg ttagtaccct tctgtatccg ttgggagagt
       61 aaggctcaac tgccccctaa aaagggttct aaaaaatctt taaaaaataa ataaaataaa
      121 cgcgcgcgcg cgagcaaaat ttcccgccaa aaaatcaccc actttttaaa ataaacaggt
      181 tcgcgcatga tattaaactg ccaatctagc tccctatggt cgctgtaaga aggagtgatc
      241 gctacgctct cacattttac ttgcagcagt cgcctttagg ctccttcgcc aaaaataatt
      301 cattagggac tcctttgtgg cgtcctgaga gctcgtctaa gccgttctaa gacaagattc
      361 gataaaaact atgtccttcg ttctaaagcg ccttacaggc ggtcagggca agccctaaca
      421 accccaaggc aataaaaaac ttaggcttat ttttgctttt ttattgtata attgattgtc
      481 gcctatccga cagtcttgcg actggaagga ggtgtgtttc gtgttcacta actttatttg
      541 ctcccttctt gttgggcttt taagcgcttt gttcagttac tggttgaata accgaaacaa
      601 gaagtaaaac ttaattaagg cgactcagcc cacaaaaaaa gccccagaga atccgacctc
      661 tctggggctt tcgtgtgttt cgtgttcact tatttgcaat caaattataa cagaaataaa
      721 tatttatttc aaaaatatcc tacaaaagtc taaaatccgt aattatttct tatatgcaac
      781 atttacaaat aaagtcgctc tgagagctcg tatttttcga tttaagacgt tttatttagt
      841 ttttggtgta tttcattggt ccaagctaaa aaactcttag aagcgatttt agagccattt
      901 aaacccactt aattatattc tccatgaaat tcgctccatg cgtcaaaagc tgcgtctgaa
      961 attccgccgt cttcgttcac tagttgcatt aatttttcca acgatcgttt tctttcgggt
     1021 gtcatatttt tatattccaa caatttttcg tctgttaaat cgaacttgat tgcttgaatt
     1081 gcttgctcta atttttctaa gttttgaaaa tattcgtaag gaaatttttc cattttttct
     1141 ttctccagtc tcacaatttt tctagaaata aaatcacttc tttgccgtct atactctcaa
     1201 aaaaaatgtc gtttgaaaaa ctaaataaat ttttgatcgt gcatttttaa atttggttat
     1261 cgattacacg tgcagcactc ccccccgaat tttaaagagc ataaggaaac gtgtacccac
     1321 tttctaaaaa aagtgggtag ataggctgtg cctatcacgt ttctcagaaa aaaatttgat
     1381 atcacttttt cgccatcgtg ggtagcttat gtatagcatt ttggcaaaaa acagctatac
     1441 aaatatagta tttgtatggt atacataaat tatacataat tgcaaaagga gattttttat
     1501 ggacatttta gttcggaata ttgatccaaa gtttgttgca acaatagaca aacgttgcca
     1561 agaactcacg aataaaacag gagaaaaatg gagtcgaaat aaatatctaa aagttttaat
     1621 cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa gaccaattcg atttattgct
     1681 cgataaattt attgctatcc aaacagaaaa tactcaaata ttaagcgagt atgtctacac
     1741 aaataatcaa atcattgaaa tgcttatcgg aggaatgata taaccatgag aaaaacttct
     1801 gtccaaaaac atgttacttt gtaccctagt catgttgacg ttttagattc catcatcgaa
     1861 aaaaatccag atatcaataa ctactcggaa gccattcgct ttttatgtat gagttatgaa
     1921 cccgatacca acacgaccac taccagcaat caggcaaaac taaatgctat gggaaaagaa
     1981 atttccatca tgactgaaat catcgcagaa tttgctgatt tatatctaaa agatacaggc
     2041 gttcttattg gcacaaattc taaagtatat caagaagtga aagaaaaaat cgaagctaaa
     2101 ctaaatccat caaatcgaaa tcgaccaaag aatcatcaaa aagaaaccag tcaaacagtc
     2161 acttctagaa atttcgaccc aacaaatttt ctaaaacgat aaaaaacaga taggagttat
     2221 tcctatctgt tttttattta gaagccccat acaatatgct agaatgtact agcaatctta
     2281 gtcggttgta agggacttgt ctacttgctt aatgtaaggg gggcgagttc ttttttattt
     2341 attcttctaa ccagttacgc agagaaatct ttggcaaatc tgacgttagg cttgaaaaag
     2401 taaattctaa tttggcgatt ttattgccct tttttgcttt tatttttgtg actttaagat
     2461 cattgaagta tttttttagt tctcgtaatg caggatttgg ttctgttgca aagttttaaa
     2521 tctactatca aataaggtag aataatagaa aaagatagca ggaggaatga cgatgaatc
//
LOCUS       DADVXO010000203         2566 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.211, whole genome shotgun sequence.
ACCESSION   DADVXO010000203 DADVXO010000000
VERSION     DADVXO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.211"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..78
                     /locus_tag="KB950_002752"
     CDS             <1..78
                     /locus_tag="KB950_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBB6858908.1"
                     /translation="LELFFAVFLFLGKNSLKQTKKKELV"
     gene            complement(108..617)
                     /locus_tag="KB950_002753"
     CDS             complement(108..617)
                     /locus_tag="KB950_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBB6858909.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(729..1727)
                     /locus_tag="KB950_002754"
     CDS             complement(729..1727)
                     /locus_tag="KB950_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6858910.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1928..2512
                     /locus_tag="KB950_002755"
     CDS             1928..2512
                     /locus_tag="KB950_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6858911.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          801 a          475 c          483 g          807 t
ORIGIN      
        1 ttagaattat tttttgctgt ttttcttttc ttaggaaaaa atagcttgaa acaaacaaaa
       61 aagaaagaac tcgtctaaat agacgttttc tttctttttt tgttgaatta ttgagtaagg
      121 cgacttcttt tttctggttc tttgtgtgct aatcgactgg cagcagcagc ggcgatcgct
      181 ccgacgatat catctaagaa ggtatggaca tgaacgccat cgtggctgtt tagttctttt
      241 aaaataccag gtttcacctt gtcgatataa ccataattcg taaagccgat cgtaccgtaa
      301 acattcacga ttgacaaggc taggatctca tcgataccgt aaaggccttc gtcatcttcg
      361 ataatatgtt gcaacggact catcaatttt ttcttttctg ctaatatatc taattggatt
      421 ccagtgataa tggcgttatg tacttcacgc tttgttagca cgctgttgac actttcagta
      481 caatccgcca aagtcaaatc agaaatatag tctttttgta aaaacatgac aagttgtgcg
      541 atatcttcta cagttacgcc acgttccttc aatagttctc ttgccttttc ttctaaagtc
      601 tcagttttga cgaccatatt attgctcccc ctttattttt tctttctcta tttcatgtta
      661 acggaacatg aaaaaaatgc aagaaactgc tttcgggttt aacttgaaat gcaaagagat
      721 ataaaaaatc aactaggacg aagattttta tcgtataagc ttgtgctgtg accaggctga
      781 gttcgttcct ttggattaat atagtgtaaa gcgttgttca ctgcagtagg tgcttcaccg
      841 agcccagtag caatcagttt cacttttcct tcataggtac agatgtcacc agctgcataa
      901 acgcctggaa tagaagtaga catatctgat tttacagtaa tggcatttct ggtactatca
      961 agtccccaag aagaaagatg ttctaaatta gaagagaatc cataattgac gatcagagaa
     1021 tcaatcatca aatcaatcgt ctcgtcactt ttcacttttt ttaaccggat atctgtcagt
     1081 tccccattat tgcctgataa tccatcgatg agatagggag taagcaagtt gacagatgaa
     1141 gatttcaagc gtgatacact atgttcgtgc gcacgaaatt ccgggcgacg atgaatcaag
     1201 taaacttcgc ttgcaatagg ctctaacatc aacgtccaat cgatagcaga atcaccacct
     1261 ccagcaatag cgactttttt tccagcatac ttcatcaaat cattcacgaa ataatccaat
     1321 ccgtggtttt caaatgattc agcattatct aaattcaatt ttcttggctg gaatgaacca
     1381 tttcctaaag ctaaaatgac tgctttggaa taatggatgc ctttatttgt agtaatctcg
     1441 ataacttcgt cttcacgagt aagcgagaga acttcttctt ttaaatggaa tgtatggtta
     1501 aaagtagtta attgtttttc taattgatcg attaattcac ttgcttttat tgctggatac
     1561 ccaggaatat cgtaaatata tttttcggga tacaaagttg ctaattgacc accaagttga
     1621 ggtagactgt caatgatttt tgttttagct tggcgcattc ctgcgtaaaa agcggcaaac
     1681 atccctacag gacctgcacc gatgattgta atgtcataga tttccataaa aattcctccc
     1741 ttttcacact atatcagaat ttaattgaga atgcttctca aaaatgtttg aattcgaaat
     1801 cgaaacggta agttatagaa tgtatctagt gatgaaaaat aaaagaaaac aaatctagta
     1861 tttttacgag aggttaggta ttatgttatt attattatga agtatgcaaa tattaggagg
     1921 aaacaatatg gcattcccac aactagattt aaaaagtgaa aaaggaccaa aagctgttat
     1981 caaaacaaac cgtggtgaca ttactgttca attattccct gaactagcac cgaagacggt
     2041 tcaaaatttc attgaacttt ccaaaaaagg ttattatgat ggcgtgattt ttcaccgcgt
     2101 tattcctgat ttcatgatcc aaggcggcga tccaactgga acaggaatgg gtggcgaaag
     2161 tatttatggc gaaagttttg aagatgaatt cagtcgtgaa ctgttcaacc ttcgcggagc
     2221 tttatctatg gcaaattcag gtccaaacac aaacggaagc caattcttta ttgtaaataa
     2281 cacaaatgtg ccagctaata tgcttggaca actagaaggt gctggattcc catcagaaat
     2341 cattgaagcg tataaaggtg gcggaactcc ttggctagat ttccgacata cagtctttgg
     2401 acatgtacta gaagggatgg acacagtaga tgaaatcgca aatgtacaac gtggtccaca
     2461 agaccgacct gtacatgatg tagtgattga aacaattgaa atttcagaat agtttggtat
     2521 ggtacggagt aattatgaaa aaggacgaaa cctagtaagg tctcgt
//
LOCUS       DADVXO010000204         2500 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.212, whole genome shotgun sequence.
ACCESSION   DADVXO010000204 DADVXO010000000
VERSION     DADVXO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.212"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1338)
                     /locus_tag="KB950_002756"
     CDS             complement(<1..1338)
                     /locus_tag="KB950_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6858912.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIER"
     gene            complement(1357..1761)
                     /locus_tag="KB950_002757"
     CDS             complement(1357..1761)
                     /locus_tag="KB950_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HBB6858913.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(1993..2250)
                     /locus_tag="KB950_002758"
     CDS             complement(1993..2250)
                     /locus_tag="KB950_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBB6858914.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          763 a          481 c          338 g          918 t
ORIGIN      
        1 tctctctatt tccttaacat actgtctaaa tcgaatctct tgataggtgg gatatgttaa
       61 agctgaatta ttttggatat caacattctt ttgtctaaaa tatctatcat atttctcaaa
      121 aagctgctgc ttatcagtgt ctttcttcca cgtttgataa agatactcta taagttcgtt
      181 gatattaaaa acttcgtcta ctttttccaa atcatccatt tgaatatatt ttaattcatt
      241 ctgtcttttc ataaccttca tgccgttgaa attcacccat tgtggtaaat gatacatctg
      301 aatataaagt tcttccaaat catctaagaa ataaaaggca ttggcacagg tcaattgaat
      361 gatatttttc aaattgccaa cattttcact aaagcgatag tttaataaaa aatctaataa
      421 ttgttgagat atcagtattt tctttgctag tctttcttct tctgatttaa aaaatatctt
      481 gattaactgt tttctttcat aaagagaacg actgtctaat gttggaagtg ttagctttac
      541 aggtattctt ctcaacaaag ttgataatag tttatcctca ggtggttctg atgttgcaaa
      601 tataatccag caacttgatg tgtaccagtt ttcattatca cccatccgat gaaatttcct
      661 cgtatccata aactgaaaaa gtttctcctg acattcattg ctcaatgcat gaatttcatc
      721 taaaaataag actccattat tagctaattc tgctaaaccc tttcttccat cttcggctcc
      781 tgtatatgca ccttttacat gcccaaaaaa attggacaag aaaagttctg gattattggc
      841 atattcacta caattaactg aaatgaaaga tgccttctct tctatataat tattggccaa
      901 caaatcctta tacacataat ttgctaaata gctttttcca gttccagtct cgccatgaag
      961 taaaatgggt aagccatttg gcggatagta gattgctgct ttaatttgag caattacatt
     1021 tcgcagactt ccttgacttc cgataaccct ttctattcca accctaaaag agctatcata
     1081 ccatttttcc gcttcctctg aagagctaaa aatttgttga tttggaactt tctccggtaa
     1141 agaaaagaag attactggac gagtattgat ttttccaaat tctccatctt ttacgaattc
     1201 attcaaatat tgagagacaa tattacgact aacatttaat ttttcagata ttttctgtgc
     1261 tgtattgttc ccccatttat ttttaatctc agagttctct atgacataat tgacaatgcg
     1321 ttcttttaat tcttccatca cagcacctcc tcgacactat tcgaattttg ccgacagtat
     1381 ttttccataa atatgacttg ctaaaaaggc tttgtttaga tcattcaatt ggaacgttgc
     1441 ttgttttgct tgatccgcta agacgttgaa aagatactct ggcgaaatcg cataccccaa
     1501 aatatcaacg attgttgcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
     1561 atacaacatc gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
     1621 aaccacttgt tccaacaaac ggtcggataa atatttatag aaaatcagcc ccaataaata
     1681 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataactt
     1741 ctgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
     1801 ccctcttatt ttagcatact cagttttaga aaaagcaaaa aaataaagaa acttattctt
     1861 gataacaagc tacgtgacgt atatccctaa cacaaaaata gccatatcct ttggtttgga
     1921 tacggctatt tgatattact cattcaatta tcaaatcaca gtaggtgtga atctcatcgg
     1981 gatcggcaat atctacttct gattaggatc aaaggtgatt ttctcaataa aggtgtgata
     2041 caaagcttta acttgcttct tctcactctg gataagaaag caatccaaac tagtcaatat
     2101 ctgttggaca tctttgactt gaatcggttc cccttgttgt tgaaagatcc ctaagatttc
     2161 attctctcga atttgtaact gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa
     2221 catcggaaac aattcgggac tatcttccaa agcagcttcc tgtttttgct ctaatagtta
     2281 gattttgcgt accattttct tcttttattt tccttcgatt gctgatttta atatagtcta
     2341 aaggaaattt atatttattg tattctttat aacttcaaga gaacttcgtt tgagtattac
     2401 tgtatttttt taattccata agaaaattag ttattacctg ccacctaatc agcaaatctg
     2461 aacctgagtc aatataatta atattatttt ccatctccaa
//
LOCUS       DADVXO010000205         2452 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.213, whole genome shotgun sequence.
ACCESSION   DADVXO010000205 DADVXO010000000
VERSION     DADVXO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.213"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(334..696)
                     /locus_tag="KB950_002759"
     CDS             complement(334..696)
                     /locus_tag="KB950_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858915.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="KB950_002760"
     CDS             complement(770..1150)
                     /locus_tag="KB950_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBB6858916.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="KB950_002761"
     CDS             1214..2407
                     /locus_tag="KB950_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6858917.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
BASE COUNT          793 a          452 c          393 g          814 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tc
//
LOCUS       DADVXO010000206         2452 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.214, whole genome shotgun sequence.
ACCESSION   DADVXO010000206 DADVXO010000000
VERSION     DADVXO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.214"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(129..746)
                     /locus_tag="KB950_002762"
     CDS             complement(129..746)
                     /locus_tag="KB950_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858918.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            complement(808..1488)
                     /locus_tag="KB950_002763"
                     /pseudo
     CDS             complement(808..1488)
                     /locus_tag="KB950_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1810..2031)
                     /locus_tag="KB950_002764"
     CDS             complement(1810..2031)
                     /locus_tag="KB950_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858919.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2129..2374)
                     /locus_tag="KB950_002765"
     CDS             complement(2129..2374)
                     /locus_tag="KB950_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858920.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          723 a          479 c          348 g          902 t
ORIGIN      
        1 aattttatat ttgctctttt cacttttcgg aataaattca ctgtgactct tgataccttc
       61 ttttatatat tgctaatgta ttttctccat tgctatgaaa atagaatcaa catatttata
      121 atatctatct aatctatttt catttcaccg cagcatctta ttaaataacg tgtaaataag
      181 aatataaaag ttattgaact aattaataaa gatagaatca tcgtagttgg atacgtagat
      241 gaaacctcaa ctatagttct tttccccgta cctttaatag acactaaacc attgtcattt
      301 tctatagtgc tctttttccc atttactttc acaactgttc cccaataatt gaatacaggt
      361 aatgtgatat tttgctcatc ggcatcttct actgtataga ctattttgga attatatgct
      421 ttatgagatg cattctccca ccgatcacca acttttactt catgtttctt taaatgatct
      481 ttatatttca atgcatctaa tggtgcatag tcatataggc ctccagttct tgaggatgta
      541 taacgtgtaa catctttttt ggtcataact aaacgacctt tatacgactg aattttatgc
      601 gaataatttg cttctgttgc tgcagtcaca gtaaaaataa taagaactat tcctacaagg
      661 ctccacatac tttttttgtt gcttgttttc cactttaaaa acactatggt taagattaat
      721 gaaccaaaaa gcacttgaaa acccaaggtt ctcggttctg ttgcgaagtt tgaaaatcaa
      781 acgcgccctc tctgaactcc aaatatctta ggctgttatt cccattaata ccttgatttc
      841 agtagacacc gaaaagccga agagcgttcc atttcttcgg ttctttttat atattcctcg
      901 aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg agactttgat aaaatttatt
      961 tcgtcgttta ataggtcgat ggtcttgttc tattaaattg ttaagatact tcacagttcg
     1021 gtgctctgtc ttagtatata aacccacact ctgtaacttt ctaaaggcgg agccaagaga
     1081 aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac tgtttatgga gtcgttttaa
     1141 gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac cagatatcta aggttaagcc
     1201 gtccgcatca attgcacgat aaagataatg ccaacgtccc ttaattttga tataggtttc
     1261 gtccattttc catgaataga aggattgtct atttttcttc ttccaaagat aatagaggac
     1321 tttgctgtac tcttataccc aacgataaat cgtagtatga caaacattta ttccacgatc
     1381 atataacaat tcctgaactt cacgatagct tagattgtaa cgcaggtagt aaccaacagc
     1441 gacaataatg acgtcttttt tgaattgttt gcctttaaaa tgattcatta ctctgtcctc
     1501 tctgtctttt ttctcaattt tacactaaaa tagatttttt ggaaaacttt gcaacagaac
     1561 cgtttttact atatacttct tcccagaact tgtccgtctc cttcaaatta tctctacctc
     1621 tcgagttcat tgggtggttt gcccatttat taaacatgga atcacctttc tcatactcat
     1681 atatttatgt cctatttcaa ttatatttat atatgcttaa ataaatttgt atcctgaaat
     1741 ttattgtaat atgaagctgt attttacata tagtaaccaa agagttaata aaataaaaag
     1801 agcgaaccat tatagatcag ctcttttttc attgctgttt ttaatcttat ctagaatcac
     1861 ttcaaatgtt tcaaaggtat ttacatctaa ctgacataac caaagcaagt acttcttttc
     1921 tttgtaagtc caatcaatat tacggtaaga ggacattaga ttatggtacc ttgctctttg
     1981 ttcaactgaa gccaattccc agcgtttttc aaatattttt tcttcttcca ttattgatcc
     2041 ctcgcaagac gctacctcaa acttaaataa tgggcagcga aagtctaatt ccttagatct
     2101 aagtatagca aacaaatatg aaaaaaactt agaaggtagt aaaagttctt ccgtcttttg
     2161 ctttgcttct attcttaatg tgtttcgtta tcggtgccct acaaagattt tcattaatta
     2221 cacactcatt tattgttgtg tattttctga catttctctc ttgatcaatt attttaattc
     2281 catgggcatc ctgatagttc ataggagagg agcggctatc agaaatagtt gcacctatct
     2341 cttgaacttc ttttacaact tctggaaaat ccatccatcc tgcttcagct gtcaaaagta
     2401 ataatctata atatttatct ttccttttca atctaagaaa tcgtcacccc at
//
LOCUS       DADVXO010000207         2448 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.215, whole genome shotgun sequence.
ACCESSION   DADVXO010000207 DADVXO010000000
VERSION     DADVXO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.215"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..652
                     /locus_tag="KB950_002766"
     CDS             <1..652
                     /locus_tag="KB950_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBB6858921.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            756..2279
                     /locus_tag="KB950_002767"
     CDS             756..2279
                     /locus_tag="KB950_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBB6858922.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          870 a          404 c          491 g          683 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat
      541 taaaggcatg gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt
      601 cgggttttcg gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga
      661 taccgtaagg gattttattc tttatttaaa actttgcaac agaaccgatc aatcgtatga
      721 tcgatcaaat ggagtctgtc aaaaaagaaa gttatttgga ccctactgtt tttgaagcag
      781 aggaacatat ccaaaaagtc attcataaaa tgttccgtcc agacaatgcc tatacaaaat
      841 ttgattttcg aaatggccgt ccaacgaagt ctgcgatctt aaccacgagt tccattgata
      901 tggccaaaaa gtattatcgg gccatcaaag agatgacgaa agaatctgat tggttaataa
      961 aagagtttcc caatcagcct atccgtgaag gacgaacgat ggaggatcct gatttcccac
     1021 gaatcgctat tacctattca ttagaagaaa atagccgaga ttcaagtgcc caacaagaag
     1081 aaatgcaaaa aattattgag gaatataatc agtattatga tactgcctgg tcactagcag
     1141 acattgagcg ttataacgga gacatcaata atcgtttggc acgaaaaaga gcggaattca
     1201 aacaatttgg gaaacaaatc gatttagtaa tcgtagtgga tcgtctattg acaggttttg
     1261 acgcccctac gattcaaacc ctatttgtgg atcgtaatct agagtatgca ggattgatcc
     1321 aagccttttc acgtacgaac cgtacgtatc ctgaaaaaac aaaaggacta attgttactt
     1381 tccgaaagcc agcgacaatg gagaagaatg tcgaggatgc gacaaaactg tattcagaag
     1441 ctaaagaaga atcgggtttg atttatccaa gttaccaaga atccaaaaaa agattcaagc
     1501 aagcatatgg taagttaaaa gaggtttcat ccatggaatc cattgatgaa catacgccac
     1561 ttgaaatccg tatcgagtac gtcaaagcat ttcaagaatt gaatcgtgcg tatgaatcgt
     1621 tagtgactta tgatgattac aatgatgaca tggtgcaatc ttcaactttg aacaatcaaa
     1681 ttcaactttt ggaagaacaa attggcgtgt acgagaccgt gaagggttca ttgatggagg
     1741 aagaaccaga aaaagaggga gaagattttt caacgatcga attttatagt gaaaatacca
     1801 ctaagttgta tgatattgat tctacttata ttgatcaatt actaggaact tatgctgcga
     1861 atagttcaga tgcacgagaa gaaattgaaa aagctttggc taaattgaat aaaacagaag
     1921 gagtcaaaga agtttaccga caaattttaa atgcatttga tgtggggact ttagatagta
     1981 atgaagatat ctttgtcatt aaacgtcgct attttacaca agcaagtgat aatttgattc
     2041 atgccttttc caatgagtgg tttgtttctg agaaagaatt acatgcttct aatctgcaat
     2101 atatgccagg tgaagatcca attcctaata tgaaagcaat tatcaacagt aaggattacg
     2161 aaggatataa agccaaacat ccagaagcga aaccatttaa atacccacaa gaaatgaagc
     2221 gagcatggcg taaaatgtta gatgatgagt tgattccact tgaaaatgaa ttgaggtaaa
     2281 cgagaagagt taagaaaatt gtacgattca aagatagtag taaagtggaa gtgtagaaaa
     2341 tagggataga tgtcttttta cttgttcaca ttttataacg gagtatttaa gaaagctatt
     2401 gaataatgat tcttccatct ttttcattca tttaccggtt ctgttgca
//
LOCUS       DADVXO010000208         2426 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.216, whole genome shotgun sequence.
ACCESSION   DADVXO010000208 DADVXO010000000
VERSION     DADVXO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.216"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..677
                     /locus_tag="KB950_002768"
     CDS             <1..677
                     /locus_tag="KB950_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBB6858923.1"
                     /translation="DLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEIYRVLLDL
                     FAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTELRSRGYK
                     LGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLEKFNLKAQ
                     ECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIRSLWEIKN
                     FLGVIS"
     gene            674..1792
                     /locus_tag="KB950_002769"
     CDS             674..1792
                     /locus_tag="KB950_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBB6858924.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1782..2402
                     /locus_tag="KB950_002770"
     CDS             1782..2402
                     /locus_tag="KB950_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBB6858925.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT          908 a          313 c          471 g          734 t
ORIGIN      
        1 ttgatttaga tgacactctt gtgtctgaat atgattatgt aaaaagtgga tacaaatgtg
       61 tagctagaag attagcagtg caaaaaattt tcccggctac ttttgacgaa atatatagag
      121 tccttttaga tctttttgct gaagatagta aaaatgtatt taatcgtcta tatgactatt
      181 ttgaaattcc ttatagtaaa aaagaaatta aagaactggt aaatgtatat cgagaacaca
      241 aaccagacat aaatttttta gaaaatgcag atactttatt aacggaatta agaagtagag
      301 gatacaagct ggggattatt acagatgggt atgctataac ccaacgaaac aaattggatg
      361 ctttgaaagt agactatttg gtagattata tttgtattac tgacgaatta ggccgtgaat
      421 tgtggaaacc acaccctatc tcatatcttt tattgttaga aaagtttaat ttaaaggcac
      481 aagagtgtat atatattggc gataatgagt tgaaagattt tataactgcc aaatcaatag
      541 gaatgcaaac aattaaaata gaaagagtaa atggtattta cagagaaagc agtttgaagt
      601 tcaaaagatt ttaccaagct gataaaaaaa tacgttcgtt atgggagata aaaaattttt
      661 tgggagtgat ttcatgaaaa gtaaaagaat tctattagct tctccacata tgagtgaaga
      721 aggatatgaa aaagagtatg taaaagaagc atttgataca aattgggttg ctcctttagg
      781 tgcgaatgtg aatggatttg agaaagaatt agcagaatac gtgggagcta gtcatgctgc
      841 cgcattatct tctggaacag cagctataca tttagctttg aaagcggctg gggtcggtaa
      901 agatgacata gtgatttgtc agtccttgac gttctcagct actgctaatc caattctcta
      961 tcaaggagct atacctgtat ttgttgatag tgattacgaa acttggaata tgaaacctga
     1021 acatttgcga acagcactag aaaaatatcc taacacaaag gctgtattag tagtacattt
     1081 gtacggttta tcagctaatt tagacgaaat tactaaaata tgcaaagaat acgatgttac
     1141 tctaattgaa gatgctgcag aatcattagg aacaacatat aaaggaaaat atactggtac
     1201 atttggcgac tatggaattt attcttttaa tggaaataag ataatcacaa cttcaggtgg
     1261 cggaatgctt gtatccaatg acaaagaaaa ggtggaaaaa gtgagatttt gggctacgca
     1321 atctcgtgag aaggcacgcc attatcaaca caatgaaatt ggctataatt atcgaatgag
     1381 caatatcgtt gctggtatcg gtcgaggaca attgaaagta ttaaatgaaa gaattgagaa
     1441 aaaacgatat attcatagtt tttataaaaa agaattgtct aaattgtcaa atatacaatt
     1501 catgccagaa aataattgga atgaaccgaa ttattggtta agtgtcataa caataaaggg
     1561 aataaatgcg aatcatctta ttgatgagtt agacaaaaat aatatagaag cccgcccagt
     1621 atggaaacct atgcatttac aacctgtttt taaaaattat gattatattg gcgatgatgt
     1681 agcagaaact ttatttgact taggagtatg tttaccatca gatacaaaga tgacagatga
     1741 agatttaaaa tatgtagtat ctacgattaa aagagtgatc aatgatgcgt aaaaaaactt
     1801 tctatcagct ttttgggaaa agaattttag atatcctatt gtcaggaatt gctctcatcg
     1861 ttttatcgcc gataatctta attgtaggtt ttttagtaag aattaaactt ggttctccaa
     1921 ttatcttcaa acaagaacga ccaggaaaaa gtgaaaaaat tttttccatg tataaatttc
     1981 gtacaatgac tgacgaaaga gaccacaatg gagaatattt acctgatgaa attcgactaa
     2041 ctaaatttgg gaaaatgctt agagcaacaa gtctggatga gctacccgaa ctttggaata
     2101 ttttgaaagg tgatatgtcg atcgtaggac ctcgtccatt attagtggag tatttgccac
     2161 tatatagtga gaagcaacgg aaaagacaca acgtaagacc aggattaact ggactggctc
     2221 aggtcaatgg aagaaatgct attagttggg aagaaaagtt tgatttagat gtttattatg
     2281 ttgataaaat tagtttcttt aatgatctta ttattataat tcagacatgt aaaaaagtaa
     2341 tcaagaaaga gaatattaat acaatcaata acaatatccc agaaaaattt aaagggagtt
     2401 aaatacagca gcttaatgca agtttt
//
LOCUS       DADVXO010000209         2416 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.217, whole genome shotgun sequence.
ACCESSION   DADVXO010000209 DADVXO010000000
VERSION     DADVXO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.217"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(1044..1709)
                     /locus_tag="KB950_002771"
     CDS             complement(1044..1709)
                     /locus_tag="KB950_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858926.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1835..>2416
                     /locus_tag="KB950_002772"
     CDS             1835..>2416
                     /locus_tag="KB950_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBB6858927.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDV"
BASE COUNT          786 a          391 c          434 g          805 t
ORIGIN      
        1 ataacatcaa ggttcctagc ctttttataa taaaatgaag cgaaaataat ttcttttaaa
       61 gagccgagat tatcagctat ataagagtga atttgatgca aaaaacagag ccgaaacagt
      121 cttggtgttc gtagctctgc ttatatgttt cgaatttaag acaaagagtt ggcaaaagat
      181 gttgataagg aagataagat aactgtgtta tataaactag gtaactaact gtaacaagct
      241 aattttaaga gattcttatt agaagacgtt taggaatata actaagaaaa tgattataca
      301 ggtaattgtc atttcaataa tgttttctaa ttttcgcgtc tttgttttaa gaaaaaggcc
      361 agaatcagtt ggaaaaaagt tcgtacgata gtgaagaggg gcaaaatttc ctactaatat
      421 tcccactaac aagagataaa acggccaggt tggtgtgatt cctctataaa aaaatgccca
      481 tgctaccgaa atcccataga ttaacaactg aacaattttt acaatgaaaa aattgttctg
      541 ccattactca aagtctcttc ctccttgtat cgtttatttg tttcatttta tcattatgat
      601 aggcgcttga acaatccatt ttgtatttta ttgagtactc ttggataaac taccagcgat
      661 tatcatattt atttatgtta ctgtaagcca tcatataaaa gtaaatgagc agatcataga
      721 aatataaaaa agaactaaaa ttgatctaaa ttatgtgcag cttgactacg taccaatcta
      781 tattacttac tagattggtc gttaatctat tcggatcgaa tgagcaaaat atttttgtga
      841 atgtccatcc ttgattatga ttccaaaaag aatcattggg aggctgtggc gtaaatatta
      901 tactataagc cagaaatacc tatcattctg aactttttta tttaggaact ttcatctcaa
      961 ggctttttca attaatttgt acccctgttt tttggataag atcatgtgca ttgatttttt
     1021 ctatctctac gttcatctcc tacttaatgc ctatctggct cctgagggct tctttttaag
     1081 gcgtcgtcat agtcttggct gaggatttct tggtaatctg agggaatggc ttcccaagtg
     1141 agcaggcgaa cgttcatgac gtgttttcct ttgtgtttga ttacagcatg gtttttctct
     1201 ttttctacct ctccagcgtg aaaagacagc catgtaccat ggccaaagat atcattacat
     1261 acccaacgtt cgatggaatt gtccacgtat cctacaggga gtgtggtata gccgatttgt
     1321 ggtttcaaaa aaggattgaa tcgatcgatc gtttcatttt tgtattgata attgataaga
     1381 aaaagataga ccaaacaaaa gacgagcaga aagaaagata gtgtcttttt gagtgagaag
     1441 ggttgttttg atgaataaga tttcttgtta acaagaggtt catctggcaa tgttggttct
     1501 attgattcgg tagctgattc aatagaagga aaatcaatcg gcagacctaa agtatgatgt
     1561 aaaagtgaca gcatttcaag gtctggcatt gtacgagaat tttcccaact agaaatcgtt
     1621 ttatcggaaa caccaatttt ttcgcctaat tctttttgcg taagcttaca atttctgcgg
     1681 taagttcgta ttttttctcc ggtgttcatc ttcttctcct ttcgattcta cagtatatag
     1741 atttattcta catgacctat acacttgata gatacgtttt tttgatgaaa aacggtatga
     1801 ttttatcaaa gaatcattta agtaagggag agtggtgata atcaaaaaaa agttcacagt
     1861 tttactcaca atcatagtga ccggtatcat gagtggtttt gccttggtta gtataaataa
     1921 acaggtactc tatcggacaa atgaattttt ccatcaaaca gatacttata ctccgattcg
     1981 attgccacaa cattttattg ggcaagaaga atatgagcag ctaatcatgg tatttgaaaa
     2041 tgtctcatca gaaactgagc tttcttatat gaaaaaaaca gtaaatgaat ccaatgaagt
     2101 agatacatct tggaacttca agatacagcc aaagcttgaa atgacattgt attatcaagg
     2161 ggatgtcaaa tcttctcacg agaaggtacc ctattctaat acctcctttc gcgtaacgtc
     2221 cattgaagaa tcgatcaaaa taaaagattt tgaaggtgaa tttttcttga tgacgagaga
     2281 aaaagaagcc tatgaacggt tcatttcact attcactgat cgattcaatc aagcatttca
     2341 cactgattat gccactaata tttttgaaga ctttgaaaat agctattcgt attcggttat
     2401 ttcaaataca gacgta
//
LOCUS       DADVXO010000210         2399 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.218, whole genome shotgun sequence.
ACCESSION   DADVXO010000210 DADVXO010000000
VERSION     DADVXO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.218"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            73..>2399
                     /gene="esp"
                     /locus_tag="KB950_002773"
     CDS             73..>2399
                     /gene="esp"
                     /locus_tag="KB950_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBB6858928.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          870 a          375 c          488 g          666 t
ORIGIN      
        1 gtgaataata cgtatcagat ttatctttta ttcttactag gaaagtaatt attaagggag
       61 ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa aacaaagaaa
      121 cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt tttgattggt
      181 gttggtcttg tcttgggaac aactgaacta gttaaagcac aagatgaaat ttctccaagc
      241 agcccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc ttcggggaat
      301 acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt acaatttagt
      361 cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga agtttacgga
      421 aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt agatgaacga
      481 ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg atcttctaga
      541 cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg gaaggtcaat
      601 tatattcgtg caagtgatgg attatttgct ggggcagaaa caaccgatac gcaaaccgct
      661 cctaatgggg taattacgtt tgaaaagagc ctagatgaaa tttttaaaga gataggcata
      721 gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca tcaagacgat
      781 gataaaattg taccgggtat tgatagtacg ggctactttt taacagattc agatgatttc
      841 tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg atcagtaagt
      901 gcaaaatatg agaaacctaa tactcaaaca aaagatggtt caggatccac aggtgctaat
      961 ggagctataa tcttagatca taagttaacg aaaaattata atttctcata ttcatcttcc
     1021 gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt tccatatgta
     1081 gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt ttcgtttgaa
     1141 tccggaacaa aagtagctga tttagcaatc gagcgacggg aaggtcatga aaattatggc
     1201 ataggttcaa taactaataa tgatttaact aaacttatcg attttgctaa tgcaagtcca
     1261 cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga aatcttagaa
     1321 gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga tttcatcttt
     1381 gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg aacaggttat
     1441 tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga gagcggagac
     1501 acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga cacaacagtt
     1561 aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca acttgttgat
     1621 aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga tggggctcca
     1681 atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt aatcgcgaaa
     1741 gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga aggttcaacc
     1801 gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg atttaaacca
     1861 gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga gtattcatgg
     1921 aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt aactgttaag
     1981 atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc tcaagctatg
     2041 gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt tcaaagtgga
     2101 aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc aatttcagat
     2161 gtctataata agtacttcgg taatgcaagt gttgatgcaa acactggtat tgttacattc
     2221 actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc tattaaaatt
     2281 gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag taaagatatt
     2341 tatgaaaatc caggagaaaa cattcctgca ggctaccaca aagtaacctt caccgcagg
//
LOCUS       DADVXO010000211         2389 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.219, whole genome shotgun sequence.
ACCESSION   DADVXO010000211 DADVXO010000000
VERSION     DADVXO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.219"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            207..2069
                     /locus_tag="KB950_002774"
     CDS             207..2069
                     /locus_tag="KB950_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6858929.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2389
                     /locus_tag="KB950_002775"
     CDS             2069..>2389
                     /locus_tag="KB950_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6858930.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          858 a          353 c          466 g          712 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagca
//
LOCUS       DADVXO010000212         2374 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.220, whole genome shotgun sequence.
ACCESSION   DADVXO010000212 DADVXO010000000
VERSION     DADVXO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.220"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..713
                     /locus_tag="KB950_002776"
     CDS             <1..713
                     /locus_tag="KB950_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6858931.1"
                     /translation="YLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLIDKKTIYEW
                     EKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGITFPDKNLF
                     QHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNILKIEELFI
                     NAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNINKLEQTI
                     SSIGKKYTKKKMKGGDTI"
     gene            752..1894
                     /locus_tag="KB950_002777"
     CDS             752..1894
                     /locus_tag="KB950_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6858932.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT          921 a          364 c          416 g          673 t
ORIGIN      
        1 attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa
       61 aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaga
      121 ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg
      181 ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt
      241 tgggaattat tctttataga gatagcattg tactagatgg tataacattt ccagataaaa
      301 acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc
      361 tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta
      421 ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca
      481 tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag
      541 aactactaga aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc
      601 ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat
      661 caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag
      721 tactccttgt aaactaagag gagatgattt catggcaaaa atatcaaagt acaaaaagaa
      781 aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga
      841 aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga
      901 cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga
      961 agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc
     1021 aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc
     1081 gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag
     1141 ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata
     1201 tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa
     1261 tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg
     1321 taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga
     1381 agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa
     1441 aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc
     1501 tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca
     1561 gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt
     1621 tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat
     1681 tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag
     1741 tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa
     1801 tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg
     1861 tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt
     1921 ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta
     1981 taacaggttt acccaacact gccttccaat gactcttttg ggaatcagag gaaagaatca
     2041 ggctttatag agatatttca tctaacaaat tactttagac tgacagtata tatcagcagg
     2101 tactgtcatc gggaacgtag ccaatatcgt atccaaaaca gcaggtagag aagctatttg
     2161 aggattcaag ttattctatt tcaaccatga agttaagttc attaaaaata aagatagttg
     2221 gtatttcaat aatttttgta tagagttaat cactttattg atgctaattc atcttgattg
     2281 gtattataat cgtattcaag ttgcgtggcg tgcttttgct agaaaatata taactgttct
     2341 ttataaagaa atataaggat taaacatttt ttat
//
LOCUS       DADVXO010000213         2353 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.221, whole genome shotgun sequence.
ACCESSION   DADVXO010000213 DADVXO010000000
VERSION     DADVXO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.221"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            49..2202
                     /locus_tag="KB950_002778"
     CDS             49..2202
                     /locus_tag="KB950_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBB6858933.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          807 a          416 c          527 g          603 t
ORIGIN      
        1 tttttgggta caatgaagca tgaatgtaaa taggcggaag gataggaaat gatgaaagat
       61 tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa taagaaaagg tgcacaagaa
      121 cctaagaaca aaacgtttcg ccggtcccat gttccttttc gcttaaattt tctttttttc
      181 attatattta cgttatttgt tgcgttgatt gctagattgg gttatttgca gatcgtgaac
      241 ggcgaagaga tggaggcgcg agtgaagtct acttctacga ttacggtcca agaaagtact
      301 cctcgcggaa tgatctacga ctccacagga aaagctttag ttacgaataa agcgaatcag
      361 gccatcacat atacgagagg aacacaagtt acggctaaag atttgcttga aatcgcaacg
      421 aagctgaact cgctgatcga tgttccggta gatgaaaatc tgacagatag agataaaaaa
      481 gatttttggc tggctgatcc caaacatttg aaagaagcca caaaaagact ttcggcaaaa
      541 gaaaagcaat tagggaacag cgaacaatat agtgcgatgg tcaataaagt cacagctgat
      601 gagattaatt taaacgagga gcaattaaaa attgctacca tctttaaacg gatgaatagt
      661 gcatatgaat taaacactgt ctatatcaaa aactctggtg ttactgatca agagttagct
      721 gttgtggcag aacgcgcttc tgaacttcca ggagtttcga ctggcacaga ctggacacga
      781 gaatataatg cagcaagttc actgaaaagt attcttggca gtgtgacgac tgaaaagcaa
      841 ggacttccag cagatgaagc agagaaatac ctagcaaaag gttattctcg gaatgaccgt
      901 gtaggacaaa gttatttgga aaaacaatat gaagatgtcc tgcaaggaac caaaacccaa
      961 tatgaagttt cccttgataa tgaaggaaat gtttcgaatc aaaaagaaat cttctctggg
     1021 gaaaaaggtt ctaatttaat gttatctatg aatgcagaat tccaatcaaa agtagaagaa
     1081 attttgaaaa ggaattatca aacattgatc aacaacggca aagcacagta ttcgcctggt
     1141 gcttatgcag tggctatgaa tcctcagaca ggagaagtgc tagcaatgac tggtttcagt
     1201 catgaacaag gttccaaaga aatcaccgag aatgctttag gcacgattac tagcgccttt
     1261 gatccagggt ctgtagtcaa ggctggtact ttgacagcag gctgggcaag taacgcgatc
     1321 agcggaaatc aagttttgat tgatgagccg attcgtctgc aaggtgcttc agaaaaaagc
     1381 tctgtcttta atcgaagcgg acaagttgct cttgatgcag tcaaagcatc ggaattgtct
     1441 tctaatacgt atatgatcaa agtggcgtta aagatgttag gactagacta tacacctgga
     1501 atgggactac ctagtttaga tgaagaagct aaagcttatc agcaattgag agactctttc
     1561 aaagagtttg gtcttggtac aacgactggg atcgatttgc cgaatgaatc tcctgggatc
     1621 tcacgttctg ttgattatat gaagaagttc aatgcggata atggcaaaga atggtatgct
     1681 ccaggtaact ttaccgattt ggcatttgga cagtttgaca catatacacc aatccaatta
     1741 gctcaatatg cttcaacagt cgctaatggc gggaaacgag tacagccgca cctagtcaaa
     1801 gcgatctatg gcaatgacga aaatggaaac ttgggtgaag tgaagaaaga aattggcaca
     1861 acagtagaaa atacggtcaa tatttctgca gaaaatatgg cgatcctccg tgaaggattc
     1921 caccaagttg tccatggtac cgatccttac acaacagcca aaccattagc cagtgcgaaa
     1981 atggaccttt ccgcaaaaac agggactgca gaaacggttg cggaaggtca tccagatatt
     2041 gcgacagtca atagtaatat cgtggcatac ggaccaacag acaatccgga aattgccatc
     2101 agtgttgtct tgccgaactt attagacgaa caagaccaca taaacttgac gattgctaaa
     2161 gaaataatgg acgcgtatta tgatatgttc atggcaaaat aaaaaagagg tcgtgaaaaa
     2221 gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat agcttctcac atttttgctt
     2281 aaatttgact tcataccgag aaactggctt ttgaacaccg tttactcggc aaaaaaggtt
     2341 gtgacaaaaa ttg
//
LOCUS       DADVXO010000214         2325 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.222, whole genome shotgun sequence.
ACCESSION   DADVXO010000214 DADVXO010000000
VERSION     DADVXO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.222"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1270
                     /locus_tag="KB950_002779"
     CDS             <1..1270
                     /locus_tag="KB950_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858934.1"
                     /translation="YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVY
                     THVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQ
                     QMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEME
                     KKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMK
                     KTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGG
                     LYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAY
                     LGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVV
                     YSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1257..>2325
                     /locus_tag="KB950_002780"
     CDS             1257..>2325
                     /locus_tag="KB950_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858935.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTM"
BASE COUNT          773 a          360 c          401 g          791 t
ORIGIN      
        1 ttatcgatcc gcaattgcag aagcaatcgc tcttacgatt atcccagctg ttttatatta
       61 tgcttatgga ttgatattca atagagaaaa aaaagtcatc gctttatctg tcagtataag
      121 tctacttgtg tatactcatg tcttaactac actattgtgt agtgcattga ttgcttgtat
      181 gattatagtt cgtttgtttt taagcaagca aataaaaaac gatataaaat ttgtggtcgt
      241 gaatttagta aaggctgttt ttttgacttg tgcattaact agctattttt ggtatcctat
      301 gttgcagcag atgaattatc aagaaataca cagtcccttc aaaacagatt tgcagctaga
      361 agcactttct atttcagata gtatcattca agcgttaaat aacgatatta caacatacac
      421 tatggggtta ttaggattgg ttgcgttgat ttttccttta ttcatactga aagaaatgga
      481 gaaaaaagaa aaaatcatct atgttggcgt agtttctaca tgggtatgcg taactaattt
      541 atttccatgg tttttgtttc aaaaaacacc gataagctta ctccagtttc cttggcgtat
      601 cttaggtatc caaatctttt tttcttcaat gatcataacg cttgttttta tgaaaaagac
      661 tatgaataaa aaatattcat ggttaatcat tggcgctact gtggtgtttc tactcgtcac
      721 gtctatagca gcaaaagaaa attacgcaaa agttatccaa tcaaaacacg gtcatattgt
      781 tatcaatgaa gaaacactcc cacgttatac aacatcagat atgggaggat tatacgatta
      841 cgccccaaca gaagcgataa aagaaaggcc tcacttagaa aaacatgaag taaaagtagg
      901 agaatcgtgg gaaaaaggga agtataaagc tgcagataat aacattactt acacagtggc
      961 aagcaacaaa aagcaaaaag tgacagtacc agtatatgct tatttaggta cacaggtaaa
     1021 agtaaacggt aaagtcgtaa atgactctta taaaaaacat ggcttagtga atgtggatgt
     1081 aaatgctgga gaaaataaga tagaaattac aacaaaatat actccaactg ctctcatggc
     1141 tttatggatc agtattctga cttattgttt agtggtgtat tcaagtttta aacgatttgc
     1201 acctaataag ttgaaagatt ctcgatatgt gactaaaaag gagataaaca agaagaatga
     1261 aaataaataa aaaagtagta atattgagtg gattaattat agttagtagt atatattctt
     1321 tcatcttttt caaaaataca cagagtattt ataccactgg agatctttct tttcacttat
     1381 ctcgaatcaa ggggttatcg actattttta ccagtccgat taattatgag acattcaatt
     1441 atactggata tggtgtaaat tatttttatc cctttctgac tttttttcca gctgtaatgt
     1501 tatattggat gacaaaaaat ttgattgtaa gctatattat ttacgtttgg ttattgaact
     1561 tgtgtacaac actcgtagct tatcattatg gcgagcggtt tttaaaacaa aaaaaagcgg
     1621 catttttatt tagttgtctc tatattttct ctgcttatcg gacagtagac atctactatc
     1681 gttccgcaat tgccgaagct atcgcaataa cacttgttat ccctgtatta ttttatgctt
     1741 atcaaatcat ctctggaaaa gaagaaaagt atccgtctgt aaagctagca ttgagtatga
     1801 gtttgctagt gtacagccat gtactatcaa cattaatgag tacagcatta attatcattt
     1861 ttatttttat tcgtttggtt tctaaaggtt tcaagaatgc agattttatt gctatattta
     1921 aaaaattatt tagtgcggca ggaatgacat tggttctgac gagtgctttt tggtatccga
     1981 tgtttgaaca aatgctctac caaaaaatca acaaacctag cgtgacaaat ttatatgctc
     2041 atgcatctaa tgtgttcgat agtctgactg aagctatgaa caacgactta actacttact
     2101 caatgggact agttggtctg ttatctttat gtattcccct gattttattt aaaaaattaa
     2161 caaacataga aaaaaagatt tattatggta cgtgtttgac ttggctagct acaactagtt
     2221 tggtgccatg gtatcttctg caaaatacgc cagccaaatt gttacaattt ccttggcgga
     2281 tcttaagctt gcaaataata ttctcttctc tgatattaac gatga
//
LOCUS       DADVXO010000215         2302 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.223, whole genome shotgun sequence.
ACCESSION   DADVXO010000215 DADVXO010000000
VERSION     DADVXO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.223"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            139..1752
                     /locus_tag="KB950_002781"
     CDS             139..1752
                     /locus_tag="KB950_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6858936.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          721 a          407 c          447 g          727 t
ORIGIN      
        1 aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta ttcaaaataa
       61 acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta cgatcaacgt
      121 agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca
      181 tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga
      241 gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac
      301 cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc
      361 tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac
      421 ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct
      481 agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt
      541 agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc
      601 gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc
      661 ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt
      721 gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga
      781 gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct
      841 gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc
      901 ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg
      961 ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg
     1021 atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta
     1081 aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat
     1141 catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat
     1201 tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga
     1261 ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt
     1321 ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg
     1381 acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt
     1441 gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg
     1501 atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt
     1561 gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag
     1621 ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga
     1681 ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg
     1741 attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg
     1801 aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat
     1861 aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt
     1921 taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac
     1981 tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag
     2041 gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga atattttttc
     2101 ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt
     2161 gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca
     2221 tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa
     2281 tttttttgtt cctatttatt ta
//
LOCUS       DADVXO010000216         2214 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.224, whole genome shotgun sequence.
ACCESSION   DADVXO010000216 DADVXO010000000
VERSION     DADVXO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.224"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            99..>2214
                     /gene="acm"
                     /locus_tag="KB950_002782"
     CDS             99..>2214
                     /gene="acm"
                     /locus_tag="KB950_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBB6858937.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          871 a          343 c          440 g          560 t
ORIGIN      
        1 aaaagattgg aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt
       61 aggttttatt tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta
      121 ttttatcaat gttatttatt attactaata taacatcact gattcctgtt catgtctatg
      181 cggatgcagg cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata
      241 ttacagatgg aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc
      301 aaccaggtga ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat
      361 tccaaaaaga aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa
      421 aagattctgc gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt
      481 gggggcaatt tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac
      541 cattcaccgt gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg
      601 gttcttcttc tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc
      661 acattcgatg gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa
      721 tcagtgatga aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa
      781 tacacttagg tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag
      841 atttccccag cgctacattt aatttttcag taactgataa ttacattgaa ataactatac
      901 caaaaaactt tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc
      961 cagaacaaat aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg
     1021 ctgttgatgg agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca
     1081 acggtacagt tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta
     1141 tcccaaatgt gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta
     1201 gtatcctgtt gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag
     1261 actatgttgt aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa
     1321 atgaactcaa attctccata aacgaaaatg atacagaggg agtcagttta cctatttata
     1381 acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa
     1441 gaccgagtat ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg
     1501 atgcagaaac aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac
     1561 agtatgatga tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa
     1621 atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga
     1681 ataaagaagt catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg
     1741 gcaaacgacc gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca
     1801 aagaagtaag tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag
     1861 acgggcaaga aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga
     1921 ttgacgggta taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag
     1981 gtagccatat aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa
     2041 atctattctc tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa
     2101 gcaattatat tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc
     2161 ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttga
//
LOCUS       DADVXO010000217         2171 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.225, whole genome shotgun sequence.
ACCESSION   DADVXO010000217 DADVXO010000000
VERSION     DADVXO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.225"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(401..973)
                     /locus_tag="KB950_002783"
     CDS             complement(401..973)
                     /locus_tag="KB950_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6858938.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(989..1594)
                     /locus_tag="KB950_002784"
     CDS             complement(989..1594)
                     /locus_tag="KB950_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBB6858939.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(1995..>2171)
                     /locus_tag="KB950_002785"
     CDS             complement(1995..>2171)
                     /locus_tag="KB950_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6858940.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
BASE COUNT          663 a          445 c          312 g          751 t
ORIGIN      
        1 tattagcagc agtgctgatt tttatgatag ttatttggtt cttaaatgaa agatctaaag
       61 acaatcaaca ccgtagggat atggagataa aatcaataga gtcaatagat tatttatcga
      121 taataacact aaaaaagcca cgttcctaaa cgtgacttac gctgaacgat atgtgttgtt
      181 tggtagacga acaaatcgtt cattataaat atatgatggt atcctaatat agtaagcgtg
      241 gctagcatac ctttaattac tttacctttt acagttacca tacacatttt ttgagcagaa
      301 atcaagtaaa taaaaaaata ttatatttca caacatgcta atagactcat atctagaacg
      361 cattaagtat ttacctgtct ggttcgctga gacttctttt ttaactttct tcaattttct
      421 tctttattct aaaaagcgta ctgcgactga aaccggttat agcttcaact tccttatacg
      481 atttacctga agtaagcagt tcatatgcgt gcctcttctt cggtgtgatt gttgctttcg
      541 gacgtccttc tctgaaatta ggattattct ttcttgcaaa catttttcct tcttgggtac
      601 gttccacaat catatcacgt tccatttctg ctacactcaa caacgtccgt gtcaccattc
      661 gccccatgga cgtattttca atcgttccta gattcaaaac cttgatcgtt acgttatcat
      721 cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt acgagcaaat cgatcaagct
      781 tcgtaacaac tatttcatct ccaggcttca gaccagccaa cagctgattg aattttggcc
      841 gatcccgttt tgtacccgta aatttttctg aaaaaatatt cttcttctct acgccgttct
      901 caagcaactg ttgaatttgt tcttttgtac tttgagaagc ggttgagaca cgtgcatatc
      961 catatttcgt catttggtcc acctactttt acaaatcatc tatcgtatac tcagaatacc
     1021 cttcatagaa atagctgaca tcgattcctt tcatatataa ctcgcgatcg tcaattttat
     1081 cagtcagagc attggaaatt agatagcgta tttccaaatc gtttatggga cttcgctcca
     1141 ttgcagaaag atagtctgct ttatcgatta agttccaatc tacaactttt tgcaattctt
     1201 cctttaaaat gagatccagc caaattctag tacttcggcc atttccttct cgaaatgggt
     1261 gagcgatatt catctccaca tatttcttga taatctcttc aaaagaagtt tgtggcatct
     1321 gatcaatatg ctgtaacgaa tgttctaaat acataactgg tgcaaatcga aaatttcctt
     1381 ttgcaatatt gaccgatcgg atttttccgg caaaatcgta aatatccgaa aatagaaatt
     1441 catgaatttc agataaacct ttaaatgtac caacttcgat ttcatttatt tttccacaat
     1501 cgtacaattc tttcgctttt tgtttactga gtttttcttc aaccttagcc aactcaactt
     1561 gattggttaa tcccaattta ttctctaaaa tcatgcgaaa tctccttaac tttatgacat
     1621 aagtttatga aacactctca accccattat atcgcgattt tgtttatgtg tcaaaaacta
     1681 ttaagtttct gacccccgat agatttttca agtgtctcta ttttgcacag tctagttgac
     1741 ctttgataca cctctatatt tatagagaat ctaaaacatc aacatggcat actcaaaatt
     1801 ctaaatatta gggataaaga attctcctat acaatcaaaa atttccttta tttcgaactc
     1861 cggtcaccta actctattga tctcttacaa gccaaagaat atctctatac tacaaagaaa
     1921 caatagggtg cctggttggt ctctttgtgt tttattttag aatctagaac gtaaatattc
     1981 ttgtcattct gattttatct aagaatatca attaaatctt tttttgcagc ctctgaagcg
     2041 ggcattacag aaaagaccag tccaataacc gaagataccc caaccgctag taaaatagta
     2101 aatagatcaa gtgacacgtt tacctttatc aaagaaccaa cactataagc aagagccatt
     2161 cctaataaat a
//
LOCUS       DADVXO010000218         2126 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.226, whole genome shotgun sequence.
ACCESSION   DADVXO010000218 DADVXO010000000
VERSION     DADVXO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.226"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(89..1015)
                     /gene="cysK"
                     /locus_tag="KB950_002786"
     CDS             complement(89..1015)
                     /gene="cysK"
                     /locus_tag="KB950_002786"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBB6858941.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1155..2042)
                     /locus_tag="KB950_002787"
     CDS             complement(1155..2042)
                     /locus_tag="KB950_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBB6858942.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          588 a          474 c          356 g          708 t
ORIGIN      
        1 aataattttt ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat
       61 aaacctatct ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat
      121 aacgttcacc attgtcggga accacagcta ataccttctt gccttttcct aattcttttg
      181 cgacttttag cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt
      241 ttcgcccaac ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat
      301 catacacaga agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt
      361 gaggtcccgc ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt
      421 cgattttcgg atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac
      481 cgatacctgc aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac
      541 cagtcgtctt ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt
      601 agccgttttc ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac
      661 caggagtcaa aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca
      721 tcgtctcagg catcacgatg attactttat atccttttgc aactcctacc atcgacaatc
      781 cgattcctgt atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat
      841 cgtgttctgc tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat
      901 taaaaaactc aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt
      961 tgacgattgg cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca
     1021 attcctcctt gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag
     1081 tgattcgtct ttgactagca aaaaaaagac aaagatatac catatatggt aatttctttg
     1141 cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata actggacata
     1201 gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg tgcaatatcc
     1261 catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt tacatctgtt
     1321 gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact tgtggttgtt
     1381 gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt aatcaatgag
     1441 ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta cgctgttgca
     1501 tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc gcccgtggaa
     1561 aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata agaagaatat
     1621 tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt ggttgccata
     1681 taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt tggcgcacta
     1741 gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat gactgaagca
     1801 tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc gataaacgct
     1861 tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg cggtaccgct
     1921 gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat cgctttttta
     1981 ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt cagctcttgc
     2041 attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt aaaacaaact
     2101 taattaatta aattatactt ttaatt
//
LOCUS       DADVXO010000219         2058 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.227, whole genome shotgun sequence.
ACCESSION   DADVXO010000219 DADVXO010000000
VERSION     DADVXO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.227"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..165)
                     /locus_tag="KB950_002788"
     CDS             complement(<1..165)
                     /locus_tag="KB950_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBB6858943.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYL"
     gene            complement(180..1130)
                     /locus_tag="KB950_002789"
     CDS             complement(180..1130)
                     /locus_tag="KB950_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBB6858944.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1127..1969)
                     /locus_tag="KB950_002790"
     CDS             complement(1127..1969)
                     /locus_tag="KB950_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6858945.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(1971..>2058)
                     /locus_tag="KB950_002791"
     CDS             complement(1971..>2058)
                     /locus_tag="KB950_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6858946.1"
                     /translation="AHGSVESVFNEENLKKAYGDTIFIGEGE"
BASE COUNT          682 a          389 c          252 g          735 t
ORIGIN      
        1 taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg gactaacact
       61 tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat
      121 tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat
      181 tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta
      241 tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata
      301 ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc tacgaataag
      361 gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct
      421 tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa
      481 tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat
      541 ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct
      601 ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg
      661 taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc tttctcactc
      721 tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc
      781 tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt ttccaagtta
      841 acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt
      901 tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt
      961 gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt taatttttcg
     1021 ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa tataaaaaga
     1081 gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat aacgatctct
     1141 ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg
     1201 ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca ataaacaatc
     1261 caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt ttcaagctat
     1321 ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt aatatagttc
     1381 ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat agataatgat
     1441 aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa gtgattaata
     1501 attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca attgtaatcc
     1561 acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat aaatccgttg
     1621 agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg aaagtaatac
     1681 caattgctgt atctcctttt attacactat tgctttttat aaaagtaata atgatagaag
     1741 ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg
     1801 ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa
     1861 taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat
     1921 tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata ttattccccc
     1981 tctccaataa atatagtatc tccataggct ttttttaaat tttcttcatt aaatacagat
     2041 tctacacttc cgtgagca
//
LOCUS       DADVXO010000220         2057 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.228, whole genome shotgun sequence.
ACCESSION   DADVXO010000220 DADVXO010000000
VERSION     DADVXO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.228"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(38..223)
                     /locus_tag="KB950_002792"
     CDS             complement(38..223)
                     /locus_tag="KB950_002792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858947.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(205..555)
                     /locus_tag="KB950_002793"
     CDS             complement(205..555)
                     /locus_tag="KB950_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858948.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(548..1873)
                     /locus_tag="KB950_002794"
     CDS             complement(548..1873)
                     /locus_tag="KB950_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6858949.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT          590 a          430 c          315 g          722 t
ORIGIN      
        1 aatgtaaaac ataaaagaaa tataatttta ttgcacttta atagccaata tagtattcgt
       61 atttatggta tcctgctgca gcttctacat agaaagagaa gcgttcaaca ccaacctcat
      121 ttatcaaacg ctggccataa ccccaaatct cttctgatag ctgatcaaag gttatatact
      181 ttagctcata ttgctggata atgttcattc taatcgaccc catttattat cctggtaaaa
      241 ttcgatatga cgtatttcgt cgtagtgaac tttgtttttt ccaacgtaga tccccagctc
      301 atcactacct aagatcttgc taacaatatc ttctggatag tatccttcag cgtttcgttc
      361 ttctttttga atacatactg atctattttt tatgactgca gcgttcaata cttccgcaac
      421 ctcttcagtc gtcatttgtt cttttgccaa attttgattg gctcgttgtt cattgtcggc
      481 atcaattttt tcagtatgat cagataaata aaaacctgcc cactttaaac gtccacgatc
      541 attataatca atcattttta agccctcctg cgccgcctgc atggccacca accaatgtac
      601 ttctagtaat actacgagcg ccttctaaca tagaagaagc atgtacaatc gacgtaaaac
      661 catatttttg ccgaatcttg tcaataattt tatctaaatt ttcttcgtca atttgctttt
      721 gtggatcact aaataaatcc agctgaagcg aatcagtgta cacaagcttg ctataggtta
      781 ttccaacgtg ccttacttct tgtccttcat aatacttatc aaacaagaaa agacagtaat
      841 tctgcaattc ctttgtatta tctgttatag ggatcttcat ctggtgtgaa aaacctggtc
      901 tcgtttctaa gatcgacgtg ccaatgtata aatggacaca acctgtttta cagttatgtt
      961 gcctgatacg aatagctact tgttccgcca tttcttttac caccagttcg atttcatttc
     1021 tcctggtata atctcgtggt aacacttgtg aattaccata tgatttttcc tttgtcggtt
     1081 ctggtgggat cgctatatct gttcgatcga tcccgttcgc atgaaaatac agctgtaagc
     1141 caatcacacc taaatggctc ttaatagtgt atggattcca attagctaag tctttaatac
     1201 tcatgatacc aatttggttg agtctttttt tcattcgaga tccaataccc caaaggtctg
     1261 tcatttcagg aatattccat actttatcag gaacattttg atacgtccat tcagcaagga
     1321 atccatcatt atgttttgct tcattgtcta aagcaagttt agctaataag ggattatctc
     1381 cgacacctac tgtagcaagc aatcctagct cttctttaat attctcctgg atcatttgag
     1441 ccagcttctt tctccgttga cttcttgttc cttcagtcgt aaaaagattc attgatctgg
     1501 tcacttttaa gatcgattca tcgatcgagt aaatcaataa atcttcgtct gctacatacc
     1561 ttctgaaaat attgtttacc tgcatatttc gcttaatata gagattcatg cgtggtggaa
     1621 cgacatgtaa cgttttagga aaaggttgcg gtagatctcg tggtcgactg acattagaaa
     1681 taccgtactt cttctttgca gctggtgaag aagctaaaat caaccctgat cctgtattat
     1741 ccgctcgact catgacgact aattcagtcg ttaatggatc taaattcctt tctatacatt
     1801 cgacacttgc gtaaaagctt ttcatatcga tcaaaaagta atcatttact ggctcttttg
     1861 aataatccat cataaataac actccattct tccacattat acaaacatac gttcgttttt
     1921 gtaaagaaga atcgattaaa tgtacaaaaa aagaagcata ggtaagttat ttccctactt
     1981 cttctgttga tttgtaaaat caacacattg tatcacttta ttaaatggat atttactatt
     2041 agtcttgtga aaatttg
//
LOCUS       DADVXO010000221         2019 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.229, whole genome shotgun sequence.
ACCESSION   DADVXO010000221 DADVXO010000000
VERSION     DADVXO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.229"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..425)
                     /locus_tag="KB950_002795"
     CDS             complement(<1..425)
                     /locus_tag="KB950_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBB6858950.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="KB950_002796"
     CDS             complement(534..1565)
                     /locus_tag="KB950_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6858951.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          622 a          330 c          348 g          719 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttt
//
LOCUS       DADVXO010000222         1984 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.230, whole genome shotgun sequence.
ACCESSION   DADVXO010000222 DADVXO010000000
VERSION     DADVXO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.230"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1087
                     /locus_tag="KB950_002797"
     CDS             <1..1087
                     /locus_tag="KB950_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6858952.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1984)
                     /locus_tag="KB950_002798"
     CDS             complement(1305..>1984)
                     /locus_tag="KB950_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6858953.1"
                     /translation="AIVLEIIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGD
                     FYEEFYKRLMRFIFVTGVRSEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETK
                     NTSSIRIISLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEI
                     AGVKPIHMHGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALAN
                     EITRFDI"
BASE COUNT          677 a          356 c          342 g          609 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gccc
//
LOCUS       DADVXO010000223         1982 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.231, whole genome shotgun sequence.
ACCESSION   DADVXO010000223 DADVXO010000000
VERSION     DADVXO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.231"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..806
                     /locus_tag="KB950_002799"
     CDS             <1..806
                     /locus_tag="KB950_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6858954.1"
                     /translation="IYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAK
                     KSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGR
                     IMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDI
                     RPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKN
                     SCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            813..1208
                     /locus_tag="KB950_002800"
     CDS             813..1208
                     /locus_tag="KB950_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6858955.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1205..>1982
                     /locus_tag="KB950_002801"
     CDS             1205..>1982
                     /locus_tag="KB950_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6858956.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRN"
BASE COUNT          774 a          217 c          397 g          594 t
ORIGIN      
        1 atatatacgg tggattggca tgtcggttaa gaaaggtttc ttatgcatta aatataaccg
       61 gattaggaac ggcatttcaa aaaaaggggg ttttgaaaag tcttattgta tttttgtata
      121 gattagcagc taaaaaatct aaggtcatat ttttcgaaaa ttttgaaaat atgaaaacat
      181 ttcagacttt acaaattgct aaacaaagta aatacaagct attaaacggt gcaggagtag
      241 atgtagaaca ctttagttat ttagaatatc ctaaagatga gcaagttcat tttttattta
      301 ttggaagaat tatgaaagag aaaggtgtag atgaactttt tgaagcaatg aaaaacttat
      361 atagtgaaga gaaaaattgt gtacttgatg tccttgggta ttttgaagaa gattacggca
      421 taaaaattga aaaatacgaa aaagagggat ggttaaaata tcatggatat caatctgata
      481 ttcgtccctt tgtagaaaga gcacattgct ttgttttgcc tagttggcac gaaggaatgg
      541 caaatactaa ccttgaatca gcagcatcag ggagaccaat tattacaagt gatatcccag
      601 ggtgtaaaga agcagttatt gaatcgaagt ctggttattt atgtgaaaga aaaaatagtt
      661 gtagtctcta taattcgatg aataaatttc taaaattatc atatgagcag cgcattaaca
      721 tgggaaaaat ggggagaaat tatatgatta aaaaatttaa taagaagaaa gttgtcgatg
      781 aaacaattaa atcattattt agttaaaaag atatgttttg caaagtatgt gatagaaaag
      841 agggattctg tggcttcaga atagatatca aagaattggc aaaagtttct gatatttttt
      901 tatttactac attacaagaa ggaatgccaa gatctatgat ggaagctatg gcaagtggat
      961 tgccatgtat agcaagtaaa atccgaggta atgtagacct tttagtggaa ggaaaaggtg
     1021 gatatcttgt tccagctaaa gatgcagaat tagtagcaga gtgtttgaga gaactaatca
     1081 ataataaatc tcttagaaag tatatgggaa atgagaacct ttccagaatt aaagaattcg
     1141 aggttagtat cgtaaaaaaa agaatcaaag aaatatatga agaagttata ggtgagagtg
     1201 taaaatgaaa aatccaatgt tttcaataat agtacctatc tattgcgttg aaaaatattt
     1261 gaaagattgc ttagattcaa taagaaatca gacatttaaa aattttgaag caatactagt
     1321 tgatgacggt agtcctgacg aatgcccaga aatatgtgac tattattccc aaatagatgg
     1381 acgtttaaaa gtaattcata agaaaaacgg tggaataata gatgcacgga aaacaggagt
     1441 cactagtgct atgggagagt atatagtttt tctagatggt gatgattggc ttgcaaatac
     1501 ttatttagaa aaatatgcgg aagtaattac taagtacaat ccagatataa tatgtagtgg
     1561 gtattttgca tcacgttcca acaaaattag aaaaagagaa ttgtcttgct cgtttggata
     1621 ttatgacaaa gataaaatta taaatttgat ttatcctaaa ttaattgagg atgaacatgg
     1681 agagtatttt agaccacaat tatgggcaaa agcttttaga actactctgt acaaggaaca
     1741 acaacaagta tctgggcaag ttagtgttgg tgaagatcat gcatgtacaa aaccatgtat
     1801 ttaccatgca aattcgatat atatgatgga aaaatgttta tattattaca gaataaacga
     1861 aaattctata acaaaaaaac cacaaccata ttattgggaa ggaccaaagg ctattggaga
     1921 gcattttgaa aagcagattg atatgaataa gtatgatttt caagatcaag tatatagaaa
     1981 tg
//
LOCUS       DADVXO010000224         1980 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.232, whole genome shotgun sequence.
ACCESSION   DADVXO010000224 DADVXO010000000
VERSION     DADVXO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.232"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            595..927
                     /locus_tag="KB950_002802"
     CDS             595..927
                     /locus_tag="KB950_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBB6858957.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1546..1887
                     /locus_tag="KB950_002803"
     CDS             1546..1887
                     /locus_tag="KB950_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBB6858958.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          688 a          265 c          421 g          606 t
ORIGIN      
        1 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
       61 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
      121 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
      181 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
      241 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
      301 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
      361 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
      421 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
      481 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
      541 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
      601 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
      661 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
      721 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
      781 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
      841 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
      901 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
      961 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     1021 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     1081 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     1141 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     1201 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     1261 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     1321 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     1381 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     1441 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     1501 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     1561 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     1621 ggatttgtga cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac
     1681 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     1741 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     1801 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     1861 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     1921 tagaaggagt cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
//
LOCUS       DADVXO010000225         1970 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.233, whole genome shotgun sequence.
ACCESSION   DADVXO010000225 DADVXO010000000
VERSION     DADVXO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.233"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..628
                     /locus_tag="KB950_002804"
     CDS             <1..628
                     /locus_tag="KB950_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBB6858959.1"
                     /translation="IIGAGGHAKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGKINE
                     LNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGT
                     VIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGI
                     GSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            618..1760
                     /locus_tag="KB950_002805"
     CDS             618..1760
                     /locus_tag="KB950_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBB6858960.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1773..>1970
                     /locus_tag="KB950_002806"
     CDS             1773..>1970
                     /locus_tag="KB950_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6858961.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVF"
BASE COUNT          702 a          256 c          413 g          599 t
ORIGIN      
        1 tattattggg gctggcggac acgcaaaagt cattattgaa accttaagat tagaaaataa
       61 gttaatatat ggattttttg atgataagca aaataaaatg caagatctga aatatcttgg
      121 gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata aatttattgt
      181 agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata tgccaagtga
      241 gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt caattggtta
      301 tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg gagagcatgc
      361 tataataaac acaggtagca ttgttgagca tgattgtaga attaataact atgttcattt
      421 atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc aaataggaat
      481 tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag gcgcagggag
      541 cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag ctaaaattaa
      601 gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat atgggaggaa
      661 cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca ttaggtgaaa
      721 atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc ggagcttctg
      781 cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat gttggaaaaa
      841 acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct attttatatc
      901 aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg agccctgttg
      961 cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag gcaatcatcg
     1021 tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt gcaaaccaat
     1081 ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat aagggaattt
     1141 cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag atcatcacaa
     1201 cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat tcaagatttt
     1261 tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata ggttataatt
     1321 accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta ttgaatgata
     1381 gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct ggaattgata
     1441 gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg tctactttaa
     1501 cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa ctaaatgaaa
     1561 aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta tttcaagggg
     1621 taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt aatagcggct
     1681 tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt attgatgagt
     1741 ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt ggtttaggtt
     1801 ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt ttactatgtg
     1861 gtgttgatac taatgaggcg agacgaaagg aagttgaaaa ggaatttgga ataccaactt
     1921 ataatagtct aacagaggca aaaaatttgt ttgaaataaa ttcagttttt
//
LOCUS       DADVXO010000226         1929 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.234, whole genome shotgun sequence.
ACCESSION   DADVXO010000226 DADVXO010000000
VERSION     DADVXO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.234"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            77..1543
                     /locus_tag="KB950_002807"
     CDS             77..1543
                     /locus_tag="KB950_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6858962.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          591 a          330 c          397 g          611 t
ORIGIN      
        1 actgtcttgt aagtttgtta aaaattgaat atgattacat ctggataaca aatttttatc
       61 ataaaggaga gaacgaatga acaacaaaca attacgctgg tttactgttg cgttaattgc
      121 atttaacatg gtttggggca tggggaatgt agttaataac tatgctcagc aaggagtatc
      181 agttgtcacc tcttggctat tgattttggc catttatttt attccatacg cattgatcgt
      241 tggacaatta ggatcagcct tcaaggatag taaaggaggc gtcagttcat gggtcgaaaa
      301 tacgacatct aataaacgac tggcatacta tgccgcttgg acttactggg tcgtccatat
      361 tccttatttg gcacaaaagc ctcaagcaat tctgattgct ggtggttggg cagtccaagg
      421 aaatggaaat atcgtaaata cgatgtcagt acaaatggta gcagttattt cacttattat
      481 atttcttgcc tttttatatt tatcaacaaa aggactttct actttgaaag tgattggcgg
      541 actggccgga acagcgatgt tcgtcatgtc gatcttattt attcttttag ctgtaacggc
      601 accgttcatc aatccaacga tggaatttgc cacaccacat atgaatcaag tgaaaacata
      661 tattccaaca tttgatttca gttatttcac aaccgtttcg atgctagtat ttgcagttgg
      721 aggggcggaa aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa aggaatttcc
      781 taaagggatg atcttcttag cagcaatggt agggatttgt gctgtattag gctcagtcgc
      841 aatgggtatg ctgtttgcta gtgataatat tcctaaagac ttgatggcaa atggggcata
      901 cagtgctttt caaattttag gtaattacta tggtatcggt aactcattga tgatcattta
      961 cgcattgaca aacatgattg gacaaatctc cgcattagcc ttttcaattg atgcaccact
     1021 aaaaatcttg ttagcagatg cggatccaga atttgtacca aactggctac gtaaacgtaa
     1081 tcgtaaaggt acattggtga atggttatct tctgacaggt atactagtag gaatcattat
     1141 tttgttgccg atttttggta tcaaagatat gaatgaatta gtaaaatggt tgacgaactt
     1201 gaactcagtt gtcatgccaa tgcgttacct atgggtgttc ttggcatata tgatgttgaa
     1261 taaagcttat aagaaattca attctgaata taaatttgtt aaaaatccaa aaatcggttt
     1321 tatctttggt gcttggtgct ttgccttcac tgcgtttgca tgtatcctag gaatggtgcc
     1381 aaaagtagat tatactgctg atcctaaagc atggtggttc caattattta cgaatatcat
     1441 cacaccaatc gtcttgatct tattaggtat gatcttgcca gcaattgctc gtcgagacaa
     1501 aaacaaagaa atcactgaac cagaaacagt ggttcagcct taaaatagaa atacttgtta
     1561 caagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattgc
     1621 ttctcaaatt tttgtgaagt ttcggcttgt ttttcgaaga agctacttct ggaacaccgt
     1681 gaatcagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     1741 agcccctcat atttttcgtt tggctgggct tattaccgca gatgcaagct ttggaacacc
     1801 gtttaaggaa gaggttgtaa aatcagcgat ttgacctgag tattagagaa acaaacggaa
     1861 aaatagctcc tcatattttg ccgctttttg agctaatgct gaaggtcagc tgattgaaca
     1921 ccatttatt
//
LOCUS       DADVXO010000227         1871 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.235, whole genome shotgun sequence.
ACCESSION   DADVXO010000227 DADVXO010000000
VERSION     DADVXO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.235"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            66..1631
                     /gene="guaA"
                     /locus_tag="KB950_002808"
     CDS             66..1631
                     /gene="guaA"
                     /locus_tag="KB950_002808"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBB6858963.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          578 a          382 c          389 g          522 t
ORIGIN      
        1 ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact aagttttatt
       61 caaatttgtc ttcaccaaca cgatttgacg aagagccaaa aattatcgta cttgattacg
      121 gtagtcagta taaccaactg atcactcgcc gtattcgtga gttcggtgtt ttctcagagt
      181 tgatgagcca ccgcattacc gcagaagaag taaaggcaat gaaccctaaa ggaatcatcc
      241 tttctggcgg accaaacagt gtttatgatg agaaggcctt tggtattgat gaaaagattt
      301 ttgatttggg gatcccagtt ttaggaatct gttacggtat gcagttgatt acttataaat
      361 taggcggaaa agttaaacca gccaaaaacc gtgaatacgg aaaagcagaa ttggaaatca
      421 cttctgcaga agcggccctt ttccaaaaca ctcctgaaaa acaaaccgtt tggatgagtc
      481 atggtgacct agttacccaa gcaccgactg atttcgatgt cgttgctacc agcaaagatt
      541 gccccatcgc atcgatccaa aatgcagaac gcaaaatgta cggaatccaa ttccatgcag
      601 aagttcgcca ttccgaatat ggaaatgact tgttgcgcca ttttacacta gatgtctgcc
      661 aatgtactgg tgactggaca atggataact tcatcgacat ggagatcgca aaaattcgtg
      721 aacaagtcgg tgacaaaaaa gttttattag gcctttcagg cggagtagat tcctctgttg
      781 ttggtgttct tttacaaaaa gcgatcggcg atcaattaac atgtatcttc gttgatcacg
      841 tattgttgcg taaaggtgaa gcagaacaag ttatggaaag tcttggcgga aaatttggct
      901 tgaacattat aaaagtcgat gccaaagaac gtttcttatc aaaacttgct ggcgtgtctg
      961 atccagaaga aaaacgtaaa atcattggaa atgaatttgt atatgtcttc gatgatgaag
     1021 caaccaaact tgccggtgaa gaaggcgttt ctttccttgc acaaggaacc ctttataccg
     1081 atgtgatcga atcaggaact gaaactgccc aaaccatcaa atcacatcac aatgtaggtg
     1141 gattacctga agacatgcaa ttcaagctga tcgagccatt gaacacattg ttcaaagacg
     1201 aagtacgtga attaggtacc caattaggca tgcctgacag catcgtttgg cgccaaccat
     1261 tccctggacc aggcttaggt attcgtgtcc ttggtgaatt gacagaagaa aaattagaaa
     1321 tcgtccgtga atcagacgct atcttgcgcg aagaaatcgc caatgccggt ttagatcgcg
     1381 acatctggca atacttcacc gtcctaccag gcattcgctc tgttggggtt atgggagatg
     1441 gccgtacgta tgactacact gtaggtatcc gtgctgtcac ttctattgac ggcatgaccg
     1501 ctgactttgc ccgtatccct tgggaagtct tgcaaaaaat cagtgtccgt atcgtaaatg
     1561 aagtcgcaca cgttaaccgt atcgtgtatg atattaccag caaaccacct gcaacagtgg
     1621 agtgggaata aagagccaac ataatatgga tgaggaatac ctataaatca acattattca
     1681 attactaaag ttcctgattt tcgtaaaaaa taagatggtt tcctaccatt ttccctacca
     1741 aagcccttgt tagattaact actaacaagg gctttgcttt tattcactac ctttattttc
     1801 ctattgaatt agctttttat ctaatcagat ttttagtact ataaatatat atatcagatt
     1861 tttcgattta a
//
LOCUS       DADVXO010000228         1839 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.236, whole genome shotgun sequence.
ACCESSION   DADVXO010000228 DADVXO010000000
VERSION     DADVXO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.236"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..722
                     /locus_tag="KB950_002809"
     CDS             <1..722
                     /locus_tag="KB950_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HBB6858964.1"
                     /translation="FLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLP
                     VLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVG
                     YSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEI
                     FLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQY
                     IGKKVKRPISADSVLLEEDVE"
     gene            719..>1839
                     /gene="neuC"
                     /locus_tag="KB950_002810"
     CDS             719..>1839
                     /gene="neuC"
                     /locus_tag="KB950_002810"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBB6858965.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDI"
BASE COUNT          671 a          240 c          359 g          569 t
ORIGIN      
        1 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
       61 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
      121 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
      181 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
      241 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
      301 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
      361 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
      421 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
      481 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
      541 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
      601 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
      661 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
      721 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
      781 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
      841 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
      901 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
      961 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     1021 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     1081 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     1141 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     1201 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
     1261 taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa
     1321 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
     1381 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
     1441 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
     1501 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
     1561 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
     1621 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
     1681 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
     1741 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
     1801 aacattgaat caaatattga atgatgggga agatattaa
//
LOCUS       DADVXO010000229         1838 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.237, whole genome shotgun sequence.
ACCESSION   DADVXO010000229 DADVXO010000000
VERSION     DADVXO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.237"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..573)
                     /locus_tag="KB950_002811"
     CDS             complement(<1..573)
                     /locus_tag="KB950_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6858966.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHH"
     gene            1265..1423
                     /locus_tag="KB950_002812"
     CDS             1265..1423
                     /locus_tag="KB950_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858967.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            1495..>1838
                     /locus_tag="KB950_002813"
     CDS             1495..>1838
                     /locus_tag="KB950_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6858968.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILP"
BASE COUNT          660 a          292 c          269 g          617 t
ORIGIN      
        1 atgatgcaca attagcactg tttttccctg atgtttcaat ttttttaacg tatccataat
       61 tattcgttca ctaactaagt ctatccctac aaatggttca tctaaaaata tgaaatccgc
      121 attttgagca agacatcttg ctaaaagtac acgttgaaat tgtcctccag ataattctcc
      181 tatttgatga ttagaatatt ttcccatatc gaccttttcc aaagcctttg aaactctttg
      241 ccattctgca tttttaattc tttgaaaaac tttcattccg gcatatgttc ccatagatac
      301 acattctttt acttttattg gaaaagtaaa atcaatatca gctttttgct ctacatatgc
      361 aatcttcacc agttttttat tgatatcttc attattaatg gagacctctc cctgatgagg
      421 gataatattt aacattgctt taagcaatgt ggattttcca gcaccatttg gaccaataat
      481 acctgttaaa gcaccttctt gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt
      541 ataacaaaca ttaagctttt tcacttctaa cataactttc tccttctaaa tgataatgat
      601 tatcacgatc atatggtata caaaaattat attttagtca accctaaatt ttatagttta
      661 gatttttttc tgttgctgaa gattagaatg acaaaactat ttgaaactac gcgtatagta
      721 agtagctttc aaaatatata aaaaaggagc gttctcaaat gagaacatgc tcctaaaact
      781 actttattgg cgtatggaaa aattatactt gaagtggata gcgggttgaa taagttgata
      841 agcatcaaaa catttataaa aattattcaa gactatgtct gttccatata caaatatgaa
      901 aatcagtaaa ctccatttaa cttttttcgt agtgtgtctt tttatacatg tttatcgtta
      961 ttaatagtga ataggaaaaa aactattttt gatcattgac aattaaatac aatttttgaa
     1021 taacgatccc tgctattcga gctagaaact caagtacgcc atgacctgat tagcgacaaa
     1081 tacttagaga tttctttttg ttctgggaaa acaaaatgcc atgacaatag cagcaaataa
     1141 tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg aaattcctat gtgcttaccc
     1201 aaagcaattc aaaaatttca taactactat tttaggagat gattttatag aatccagagt
     1261 ctacctgaat aaaaaacaaa cctgtgcatt tctaggaatc gataataata ctctagataa
     1321 gtggattcgt atatataatt tcccagtaat aagagttggt ggtgtagttc gctttgacgt
     1381 agaagatatt aaagacttta tgaatacatt taaaataact tgaaattttc ttctcgccgc
     1441 atatactaaa attagtgaaa tatactgcct tacttttttt aaggagtgag gattttgcct
     1501 acaaagcttt caaatgggaa atataaaact aatctgagat atccaaaaag gtttaaagaa
     1561 ataacaggaa ttgcatcaga aaagtatcaa aaaacatttc caaataggca attggcgatt
     1621 aaagccgaaa atgatatgaa gaagaaaata gaaaaagttt tgagagagga gaatgcaaac
     1681 tcattagaat taaaaggaaa aataacgttt aaaaaatttt acgaatctaa atggcttcct
     1741 agatatgaat taggtcaaac aattagatca aacaggcctc ctagtgatat aaccattagt
     1801 aatacaaaag atatatttcg gttgcatatt ttacctat
//
LOCUS       DADVXO010000230         1827 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.238, whole genome shotgun sequence.
ACCESSION   DADVXO010000230 DADVXO010000000
VERSION     DADVXO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.238"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..502
                     /locus_tag="KB950_002814"
     CDS             <1..502
                     /locus_tag="KB950_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB6858969.1"
                     /translation="SVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVS
                     VYTSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPL
                     QYLEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIF
                     FLSILI"
     gene            513..>1827
                     /locus_tag="KB950_002815"
     CDS             513..>1827
                     /locus_tag="KB950_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6858970.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCN"
BASE COUNT          661 a          233 c          315 g          618 t
ORIGIN      
        1 ttcagttctg tttttagtaa tgaaaattat tgatgctatc aagatagagt tttttactat
       61 attttttgcg acatccttat atattgtatt tcgaatgttg ccggtcgtaa tgatctcatg
      121 gttgttggtt agtgtctata ctagcaatga attattatcc tctttagaaa aaatacattt
      181 accgaaaaaa ataatgttaa gtattacagt agtgatcaga ttctttccga cttatcgttc
      241 tgaaataaga atgattaaag aatctttaaa aatgagaaac attcgcttgt ctttttttca
      301 gcctctgcaa tatttggaat ttctgttagt tcccgttctt atacgtgcaa ctatgattgc
      361 tgaagaaatg accgctaatg caataacaaa ggggatagaa tctcctgtga aaagaagttc
      421 ttactataaa acaaacatga gcttattaga ctatatcatc ctgttttccg ttactatttt
      481 tttcttaagt attttgattt gaggtgaaag aattgtttga atttaacaat gtttcttttt
      541 catataagga tgttaaggaa caagcgcttg ttgaccttaa tttttcaata aaaaaaggca
      601 gtcttacatt gattactggt tcttctggga gtgggaaatc cacactatta aagttaatga
      661 atggacttat tccaagactt tatgacggaa aattggttgg ggaaatttta tttgaaaata
      721 actcgctaca atccttaaca caagaaagta tatcaaaaaa tattggctac gtaagtcaag
      781 atcctagagg acaattcttt acaacaaata caacgagtga gttagtattt tcgatggaaa
      841 actttggatt tacaaaagag catatggata aaagaatcaa tgaattagct gaattattag
      901 atctttcaaa tatattagat aaaaacattt tttcgctatc aagtggtgaa cggcagaaaa
      961 tttctattgg atgtagttta tcgctaaact ctaaattaat tatattggat gaaccgtctt
     1021 caaatttgga cttcaaagcg actaagaaac ttgctgactt actttgtgat ttaaaaaata
     1081 aaggatttac tattgttata gctgaacatc gtttttacta tttgaaagaa ttaattgatc
     1141 aagttctatt tgttaaaaat agaactgtaa aaactacaac tattcagttg ttaaaaactg
     1201 aaaacaataa cgcattaagg aaatttgaca tttttaatag ttcgattgaa gttccctata
     1261 tcgaaaaggt aagtgcagat gtattaaaag ttgaagaaat tacttatgaa aatattctaa
     1321 aagacataaa ttttgatgtc aaagttggag atgttattgg tttggtaggg aaaaacggtg
     1381 tggggaaaac aaccttacta agaatactca tgaagattat tcgccctacc aaaggaaaga
     1441 taatcgaaaa taaaaaaaat ttgtcatcgc cgtttttagt aatgcaagaa atggattacc
     1501 aatttttcac agaatcagtg aggagcgagt tagccctggg gaatgaaata gttagtaaag
     1561 atcagcaaga aaaaatttta aaaaaaatgg aactttataa aatgaaagat caattaccgt
     1621 ttgatttatc cggtggagaa aaacaaagat tattggtttc tatagcttca ttgtcaaaaa
     1681 ctaatttatt tctatttgat gaacctacaa gtgggttgga ttatattaat atggaaagga
     1741 ttgctgacct tattaaagac ttaaaagaaa agggtgcagt agttatagct actcatgatc
     1801 cggaatttct ttataaaaca tgcaata
//
LOCUS       DADVXO010000231         1819 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.239, whole genome shotgun sequence.
ACCESSION   DADVXO010000231 DADVXO010000000
VERSION     DADVXO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.239"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..475
                     /locus_tag="KB950_002816"
     CDS             <1..475
                     /locus_tag="KB950_002816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858971.1"
                     /translation="DILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNS
                     NQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAE
                     VIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            582..>1819
                     /locus_tag="KB950_002817"
     CDS             582..>1819
                     /locus_tag="KB950_002817"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6858972.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT          575 a          258 c          314 g          672 t
ORIGIN      
        1 agatatttta aaagcaattt ttcaaagaat tggaaatgaa aatgcagtaa tatttgctaa
       61 aattagagat aacgatagtt taaacggttt agaaaagtta agagaaatat ttaagacagc
      121 ggtattcaat tctaaccaaa gcgttttatt gacagtttct cctcaactat taaacaatcc
      181 tcgttttctt gctatgcaaa tagaacaaat gtatgaaatt gtagtacctg aatttgttga
      241 acccgtttta gaacaaggta tcaaggataa gtctttggag attgaaaatt cacatgaaat
      301 tgcagaagtt atcatggtat taagtaatgt atggctcaac ccattagttg aaatgacgga
      361 taaagagggt atggaaaaac gttgcaatac attcaatgcc cttttaaatg gaataggcat
      421 agacacatta ttagacgaag aaacaatttc tgcatttata cagttttgta aataactgta
      481 tttgttatcc ctatacgata gactgctcta taaaagagca gtttatttaa accattatat
      541 aaaccgacgg tctgtttgat taaataaaaa ggagagattt tatgcaagca gaaaaaaaac
      601 tttttaatcg tgattttata cttgttgtta taggacagat aatttcttta tttggaaacg
      661 ctatcctacg atttgcatta ccgtactatt tgttaaaaga aactggttca tcagctttgt
      721 ttggtattgc tacagcctgc tcttttttac ctatggttat tttgtcgctg gtaggtggta
      781 ttattgctga caggacaaat aaaagaaaca ttatggtcat actagatttt gtaacagcaa
      841 tcataatttt agtattttat ttgtcattgg gaaaaattcc tatcatacct ttgtttattg
      901 ttgctttgat gttactttat ggtatttctg gtgcatatca accagcggta caagcaagta
      961 ttcctctatt ggtatcatca gataaactta ccgtcggtaa tgctataata aatcaagtaa
     1021 atactttagc aaactttatc ggaccagcta taagcggagt agtttttcaa ttttatggta
     1081 tcaatcccat tttaattgtt agtattatat gttttacttt ttcagccatt atggaaattt
     1141 ttatccatat tccacaccaa aaaagacctc gtgaagtagg tgtaattgca gttgctaaaa
     1201 acgatttaaa agatagttat acctttttga aaaaggagaa acccattttt atatccgtaa
     1261 tctttttggt ttgcattttc aatttggttt taagtgctgt tttaattgtt ggtctgccta
     1321 tcttaatttc tgaagtttta aaaatgccag atacactata tggtttttca caatcagcat
     1381 tatcattagg tggtttatgt ggtggtattt taacagctct tgttgcttca aagttaaaac
     1441 taaaaaacct ttataccttg ttacttattt gttctgtttc tgttttactt atgggaattt
     1501 cgctattttt agaattgcct gcaatagttt cctactgggt agttacagtg atgtgctttg
     1561 tggctatggg tgtatctact atgtttgttg ttcaaattta cactcttgtt cagacacaaa
     1621 caccaccgca attagtagga aagataatgg ctacgctggt agcagttgct atgtgcggtc
     1681 aacctattgg acaaatggtt tacggtattt tattcgatgt atttatacaa gaaacatggg
     1741 ttgttatgtt tgtagcttct attgcttcaa tcatgatttc aatatattca aaaagaattt
     1801 ttcaaaagtt agagatata
//
LOCUS       DADVXO010000232         1746 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.240, whole genome shotgun sequence.
ACCESSION   DADVXO010000232 DADVXO010000000
VERSION     DADVXO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.240"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            45..611
                     /locus_tag="KB950_002818"
     CDS             45..611
                     /locus_tag="KB950_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6858973.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            756..843
                     /locus_tag="KB950_002819"
     tRNA            756..843
                     /locus_tag="KB950_002819"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:788..790,aa:Ser,seq:cga)
     gene            1177..>1746
                     /locus_tag="KB950_002820"
     CDS             1177..>1746
                     /locus_tag="KB950_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBB6858974.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          596 a          272 c          342 g          536 t
ORIGIN      
        1 caataaaaca acatatactg cagtaaagaa gtgggggagt tttaatgaga agaaaagtct
       61 ataccaagga tcaaattcta aaagctgctt atgatgttgt tgcaaaggaa gggtttaaag
      121 gatttactgc gagaaatatt gctaaaaaga tggggatttc gacacagccg atttatctgg
      181 aattcaaaaa catggaagat ttaaaaaata cgttgctgga ttctatcttt gaacaactaa
      241 aagaagatgt tttccctatt gaacgtactg gtgataaaat cattgatctc ggtttgaact
      301 atatctactt tgcaaaaaag catcatagtc tttacatggc attgtacatt gatgaacatg
      361 gggacggaaa gaaaatgcat gatttttcat acaattattt ccgtgaaata gttaaagaag
      421 atgaaagata caatcaatta tcggatgaat atgtcgatgc tctgcatact ggtacatggg
      481 tagcagtgac tggcttagcg tcattgatgt catcaaacat catgcatcca acagaagaac
      541 aaatcattgc actgatcaaa cggacaattg actcgatttt agcattagat aatcctgaaa
      601 taatcagata gtgttctatc agtatgaatt tgatcggtaa gtccgttggc tgcaaactgt
      661 caatggactt ttttgctaaa aaaagcgatt tctgttattt atctattaga aataggcttg
      721 acatttcgtg aaaaaagttt aaaaatagta cttacggaag gttggcagag tggtaatgca
      781 ccggactcga aatccggcga accggctaat accggcgcgc aggttcaaat cctgtacctt
      841 ccttatatca acgattagag gttgtgaaga agctgtcctg catcaagtaa taagaacggc
      901 caaacaaaaa tagcttttcc tattttttcg tttgattggg cttattaccg cagatgcaag
      961 cttttgaaca ccgtttgttc ggaataaggg gctgtgacaa gacttttgtc acagcccctt
     1021 attgttgtac ttttaaccta caatattttt tgattccccc tgagtactat tttttataaa
     1081 tgctagtaaa tcaatcttct ttggcctttt tttgataaaa aaaatactag atgtaagata
     1141 aaagtgtaaa aacgatttgt tttaggaggg gaaacgatgt gtaccgcaat cacttatgca
     1201 acgaaagacc attattttgg aaggaatttt gattatgaga tgtcttataa tgaagtggtc
     1261 actattactc caagaaatta tcgtttcgat tttagaaagg taaaaaattt agataaacat
     1321 tatgcaatga taggaatagc agctggggta tccaactatc cactctatta tgaggctaca
     1381 aatgaaaaag ggttgagtat ggcaggactg aattttcctg gcaatgctga ttataaagaa
     1441 ttacaagaag gaaaagataa cgttgcacca tttgagttta ttccatggat actagggcaa
     1501 tgctcaacca tagacgaagc taaagaatta ttggctacta tcaatctagt caatatcgat
     1561 tttagtgaaa aactaccctt gtcaccttta cactggttat tagctgataa agaaaaatcg
     1621 atagtaattg aaagtatgaa agatggactt catttatatg ataatcctgt cggtgtgttg
     1681 acaaataatc cgccatttga ttatcaatta tttaatctga acaattatcg ctcgctatca
     1741 aatgga
//
LOCUS       DADVXO010000233         1705 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.241, whole genome shotgun sequence.
ACCESSION   DADVXO010000233 DADVXO010000000
VERSION     DADVXO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.241"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(943..1356)
                     /locus_tag="KB950_002821"
     CDS             complement(943..1356)
                     /locus_tag="KB950_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBB6858975.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          650 a          270 c          254 g          531 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaa
//
LOCUS       DADVXO010000234         1695 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.242, whole genome shotgun sequence.
ACCESSION   DADVXO010000234 DADVXO010000000
VERSION     DADVXO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.242"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>1695
                     /locus_tag="KB950_002822"
     CDS             <1..>1695
                     /locus_tag="KB950_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6858976.1"
                     /translation="FDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQS
                     RTNRLGRRVVTLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLN
                     CIAGEQGSLLQYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKT
                     RIIPYADPQSEVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAK
                     NYFKTINPGCDKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLES
                     KVSGLQYDSLSEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMI
                     TGQGSAGGYVVLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAW
                     TLDSIFNANFIQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEF
                     WNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLL
                     GNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          627 a          212 c          338 g          518 t
ORIGIN      
        1 atttgatgaa tattttgaaa atgactatca aataaaagca aagccaaacg atcaagaaga
       61 gtatcatatc ttttttattg atgataaaga tatagatact tttttaaata cagtaactat
      121 ttatgctcag agtcgtacaa atcgacttgg aagacgggta gtcactcttg tggaagtgga
      181 ctctaaaact ggtcaagaag cgatatcaat tattgaaact aaaatggata aaaaatctga
      241 catacgactt tattctgata ttacggctgt ttctagcaca atctttgaag cgagaaatgt
      301 tttaaattgt attgctggtg aacaaggatc attgcttcaa tattggggcg gagaaattaa
      361 acgtgaacca tttaagcttt ctttgttaaa gcgaagaggt cgcaataatg ttggaacgat
      421 tcggtatgga aaagatatgt ctggtttgaa ggtcaaatta gattggacag gtataaaaac
      481 aagaattatt ccatatgctg atcctcaaag tgaagtaggt acgactaatc gaatttatgg
      541 ttcgccagta gatagtgcct atattaataa ctatcctgat gtctatacag aacatgttca
      601 gtttacagaa gaacaaggag taaaagacat taagagctta aataaaatag ctaaaaatta
      661 ctttaaaacg atcaatcctg gctgtgataa gcctaaagtt tctattacgg ttgaatttga
      721 taagttgact gatagtgaag aagcgaaaga atttgcgaag attagaaatt atggtctgtt
      781 tgatacattt aaaatatatc ataaaaaata taatatttat cttgaatcaa aggttagtgg
      841 actacaatac gattctttat ctgaaaaaac tttgaaatta gaagcaggag acattcaagt
      901 tgctttttat cagcaacaag ctgtaactat tcaggataaa ttaaaagatt atgcaacgaa
      961 taattatatg agtgatttta atgattatgt ttcttcaatg attacaggtc aaggcagtgc
     1021 aggaggatat gtagttttat ggccaaaaga aaaaccttcc aacatcttta ttatggataa
     1081 tccagattta gaaaaggcaa gagaagtact aagaatgaat aaaaatggga ttgctttttc
     1141 taagaaagga tggcgggggc cttttaattc agcttggaca ttagatagta tatttaatgc
     1201 caattttata caaactggat taatcaaagc agatatcttt caaaattctt ttaacaagac
     1261 tggagatgta ttgaaattag ttaatggact actccaaatt tggaacaaca aaaagaaaat
     1321 aatggagtta actaaaaaag gaatggaatt ttggaattct aagggttcaa ttggaacgat
     1381 tggaacaact gattctgttg gtaatccttt tcctggggct tctactccaa cccctattga
     1441 agataattct ttagttattc gtacaaatgg agacggcaaa tatattttga tttctcctaa
     1501 agtaggaaaa ggattagttt tattaggaaa tggtaaagca atttattttg gagacttaga
     1561 tgtacaagga aaacttacag ttaaaggaaa agaaataatt cctggacaag gtggtggtcc
     1621 atcgggaggt ggagaatcta ctggtgggta tcctagtgaa ttaaaaacag atgcagaaaa
     1681 gagagcttgg agaat
//
LOCUS       DADVXO010000235         1678 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.243, whole genome shotgun sequence.
ACCESSION   DADVXO010000235 DADVXO010000000
VERSION     DADVXO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.243"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1442
                     /locus_tag="KB950_002823"
     CDS             <1..1442
                     /locus_tag="KB950_002823"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBB6858977.1"
                     /translation="ESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPE
                     YLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVT
                     ITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVT
                     LDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHG
                     VDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLA
                     PKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMP
                     ANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAER
                     KDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEK
                     ELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1435..>1678
                     /locus_tag="KB950_002824"
     CDS             1435..>1678
                     /locus_tag="KB950_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBB6858978.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          588 a          280 c          355 g          455 t
ORIGIN      
        1 atgaatcctt agaggaatat gatacggtaa gcaaacaaac gatgttgtat cgcgaattac
       61 tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt ttggggtatg
      121 cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc
      181 aattgaatga tctaaacaaa gcctttgtgc aattggcatc tacctacaat caatttaatg
      241 ggttatttga tgatgtggat ctccaatcaa aaaaactagg aacagatgag caacaacgaa
      301 atgtgacgat taccgaagtc attaagaaat tgaatgacgt ggaagtttta ggtcacgatg
      361 gggacgtgat cggggatgct tatgaatttc tgatcagtca atttgcttca gaagcaggga
      421 agaaagccgg agaattttac acgcctcata tggtttctga tatgatggca caaatcgtga
      481 cgttggatca aaaagaacga cgattcttta gtgtttttga tccaaccatg ggttcaggtt
      541 cattgatgtt aaatgtgcgt aactacttaa ctcacccaga taacgttaaa tatcatgggc
      601 aagaattaaa tacaacgaca tacaatctag caaaaatgaa cttgatttta catggggtag
      661 atgccgaaga aatgaatctc cgcaatgggg atacgttgaa taaggattgg ccaacagatg
      721 aaccttacac atttgatgca gtagtaatga acccacctta ttcggctaat tggtcagcag
      781 atacgacctt cttagacgat tctcggttta atcgttatgg aaaattagcg ccaaaatcca
      841 aagcagattt tgcgtttctt cttcatggtt tctatcattt gaaggaaaca gggactatgg
      901 ccatcgtctt gccacacgga gttttattcc gtggcgctgc agaaggggtc attcgtcaaa
      961 aattactaga agacggcagt atttatgctg tgatcggtat gcctgcgaac ttgttcttcg
     1021 ggacatcgat tccaacgacg gtgattgtat tgaaaaagaa ccgccaaact cgagacgtat
     1081 tattcattga cgctagtcga gaatttgtca aaggaaaaaa tcagaataaa ttatcggaag
     1141 aaaacattca aaaaatcctc gaaacctatg ctgaaagaaa agatgtcgag aaatacgctc
     1201 atttggcaaa ctttgatgaa atcaaagaaa atgactataa cttaaatatt ccacgctatg
     1261 tagatacgtt tgaagaagaa gaaccaattg atatggttca tgtcggaaat gacatcaaga
     1321 agatacgaca agaacagcaa gtgctagaaa aagaactttt agaagctatc tcttccctgc
     1381 aaacaacacc tgaaaatgaa gcgtggttac aaggtgcgct ggaggtgttc aaacatgagt
     1441 aatgatatac aaccagaaat tcgtttccca ggatttacgg aagcttggga acagcgtaag
     1501 tttgaatgct tacttgataa aaaagatgga gtaaggaggg gaccatttgg gagtgcctta
     1561 aagaaagaat tctttgtatc taatagcaat tttgttgtct atgaacaaca aaatgctatt
     1621 tatgataatt acgaaacaag atataaaatt actgaaaaaa aatataatga attaatca
//
LOCUS       DADVXO010000236         1664 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.244, whole genome shotgun sequence.
ACCESSION   DADVXO010000236 DADVXO010000000
VERSION     DADVXO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.244"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            131..505
                     /locus_tag="KB950_002825"
     CDS             131..505
                     /locus_tag="KB950_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6858979.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="KB950_002826"
     CDS             complement(619..1248)
                     /locus_tag="KB950_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6858980.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="KB950_002827"
     tRNA            complement(1382..1454)
                     /locus_tag="KB950_002827"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DADVXO010000237         1585 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.245, whole genome shotgun sequence.
ACCESSION   DADVXO010000237 DADVXO010000000
VERSION     DADVXO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.245"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1477
                     /locus_tag="KB950_002828"
     CDS             <1..1477
                     /locus_tag="KB950_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858981.1"
                     /translation="KKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARVKGLS
                     SIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNVCTIM
                     LMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYYAYAL
                     IYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRLIVEL
                     SKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDLTTFT
                     VGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMIQFPW
                     RILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKVVTQR
                     DHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQVMDSK
                     IVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
BASE COUNT          487 a          261 c          286 g          551 t
ORIGIN      
        1 gaaaaaaata atattgtcca ttctcctagc aattattgct ggactttatt caatcaatta
       61 ttttagaaat gttcatacta tttctacatc gggggatatt gccttccatt ttgctagagt
      121 caaagggtta tcttcaattt tttctggtcc aatcaatttt acgacgttta atcattatgg
      181 gagcggagtg aattattttt atccctactt gacctttttt ccggcagtca ttttttattg
      241 gataagtaat aatttaatcg ttagttatat cttatatgtt tggctattga atgtttgtac
      301 tatcatgctg atgtttcatt atggactaaa atttttaaaa agaatcgatg cggcttttat
      361 atttagctgt ttatatacat tttatgggta ccgtacgatt gatatttatc atcgttctgc
      421 tattgctgaa gcgattgcac ttacggttat accgatagtt atgtattatg cttacgcact
      481 gatttatgag aaaaagccat gtgcaattcc attagcagtc agtatgagct tactcattta
      541 tacacatgtt ctttccactt ttatgagtgt aatagccgtt ggcttactag tcttaggcgg
      601 ctttgtagta agtgaaacaa gaaaaaaaga aagcatccgg ttgattgtgg agttgtcaaa
      661 ggcagtagga atgacgattc ttttaacagc atacttttgg tatccgatgt tccgacaaat
      721 catggttcaa tcgattaatg agccagataa aacgacctta caacaccaag cattaaatgt
      781 ctcggatagt ttgattggtg cgatgaataa tgacctgaca acctttacgg taggaatcat
      841 tggtattatt tctttgatct gtccactctt cttttttaaa ggatttaatc gcaaagaaaa
      901 agtgatttac cttagtgcgg tagtctcttg gctcttatcc accaaatttt ttccatggtc
      961 tttattacaa aatacaccca ttcaaatgat tcaatttcct tggcgaatct taggctttca
     1021 agtactgttt ggctcgttga ttttatcgtt cgttttcatg aagagcacat cagctaagca
     1081 aagaaaagta gttaatataa cggcaattgt tttattactg ttggctgtat ctgctgcaac
     1141 aaaagcaaac tatgaacata aagttgtcac tcagagagat cacttggtaa tgaatcaaga
     1201 aaatcttatt cgttacacga cactccttcc cggaggactt tatgactatg cacccacgga
     1261 agcattacaa tacaaagaac atttgcagaa tcatgaagta agaattggca aggaatggca
     1321 tactatgcct tatcaagtga tggattcgaa aatagtctat acagtacatg aatctaaagg
     1381 acaaaaaata acgcttcctg tatttgatta ttggggaaca gtcgcaaaag taaatggaca
     1441 aaaaagagag attgataata ggactagtgt cgattgaagg aacacaagga gaaacaatca
     1501 ttgaagtttc ttccatttat cctccaacta tgaagttagc ttttttaatt agtttatgta
     1561 gttacctctt tcttttaatt agttt
//
LOCUS       DADVXO010000238         1573 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.246, whole genome shotgun sequence.
ACCESSION   DADVXO010000238 DADVXO010000000
VERSION     DADVXO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.246"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            29..1108
                     /locus_tag="KB950_002829"
     CDS             29..1108
                     /locus_tag="KB950_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6858982.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            1349..>1573
                     /locus_tag="KB950_002830"
     CDS             1349..>1573
                     /locus_tag="KB950_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HBB6858983.1"
                     /translation="MWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWYSCISMFVF
                     HIGALLFTRKTTLRKVSWYESGDADADRKFL"
BASE COUNT          589 a          182 c          309 g          493 t
ORIGIN      
        1 atatataatc gataaagagg taggaaatat gaggaaattg acctttgtga taggaattat
       61 gggaaaagga ggggcagaac gagtaattgc gaatttggca aataatttta ttcaagataa
      121 ctgggaaatt gatattataa ctatatatgg gaatcggcaa gactatgaac tgaatagaaa
      181 aatacaggta catccaataa tatgtaaaag ttcgtttcgt gcatttagac ctttggaacg
      241 ctttaaggaa cttaggaaaa aaataaggga aatacagcct gatctggtta tttcgttcct
      301 tgctgatgta aatatccatg tcattatttc attgttagga gtaaaacaaa aattaattgt
      361 ttctgaacga aatgatccac aacggaatcc agaagaacca tggattagga aaattagaga
      421 ttttctttat aaaaatgtag atggaattgt ttttcaaaca ccagacgcaa aaaaatattt
      481 tagtaagatc aatgataaaa aagtaaaaac aattataatt cccaacccaa taaaggaaaa
      541 tttaccttat tataataaaa ctacaagtag taaaaaattt attactgcat gtagattgga
      601 taaacaaaaa aatttgccct taatgattga tgcaatagga aaacttattc atgaaggata
      661 tgagtgtgaa ctggatattt atggagaagg gggattaaaa accgagcttg aaagttatat
      721 caaccataat ggattaacag aatttgttca tctaaaagaa tttacaaata aaatacatga
      781 aaagatgcaa aatgcggcag gatttgtaat ttcttctgat tatgaaggta tatctaattc
      841 tatgttagaa gcactggcta ttggagtgcc agttatcgct acagattgtc cagtaggtgg
      901 cgcaagaatg tatttaggtg atagaaatga aataggtcat cttgttgaat gtaataatct
      961 agataagtta acagatgcaa tgcgtagggt acttttggat agagaaagtg tggtaagaaa
     1021 agcgcaaaaa ggaattaaac taagaaaaga actgacatct aaaaatattt caaatatatg
     1081 gtacgaatat ttaaaccagt tcttttaacc aaaaggagga tattgtgtga aagaaaaaat
     1141 aaatatattg atgttttcgt taacgttagt cgcgtttaca atattttgcg ctttttcatt
     1201 aaatcagaac tattggctaa attggcttgg aatattgatt tacttatatg caatctgtac
     1261 atggcattgg agaaaaggag attcaatatt ttcattatac acgatttttt tcactctttt
     1321 tttattattt agttatgggc aatgcattat gtgggcattc ggtattggaa cagatcgtgg
     1381 aatcggtaca acagtagtag cttatggtac ggggattatt cctagtgaat cagatatgat
     1441 aatggtaaaa tggtattctt gtatttctat gtttgtattt cacataggag cactcttatt
     1501 tacgagaaaa accacactaa gaaaagttag ttggtatgaa tctggagatg ctgatgccga
     1561 tagaaagttt tta
//
LOCUS       DADVXO010000239         1563 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.247, whole genome shotgun sequence.
ACCESSION   DADVXO010000239 DADVXO010000000
VERSION     DADVXO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.247"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1097)
                     /locus_tag="KB950_002831"
     CDS             complement(<1..1097)
                     /locus_tag="KB950_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBB6858984.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>1563)
                     /locus_tag="KB950_002832"
     CDS             complement(1227..>1563)
                     /locus_tag="KB950_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBB6858985.1"
                     /translation="LIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAK
                     QSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRN
                     PKELLIDNN"
BASE COUNT          482 a          317 c          265 g          499 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 agg
//
LOCUS       DADVXO010000240         1549 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.248, whole genome shotgun sequence.
ACCESSION   DADVXO010000240 DADVXO010000000
VERSION     DADVXO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.248"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(68..1503)
                     /locus_tag="KB950_002833"
                     /pseudo
     CDS             complement(68..1503)
                     /locus_tag="KB950_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          498 a          262 c          259 g          530 t
ORIGIN      
        1 tcatttgtta cactctcaat cttagctgtt gttctattca tcattactct tattgtaagt
       61 agttcagtca aatttgcttg ttcaattgat cgataacagt atgaattgtt tgaaaagtaa
      121 tcggatatat aattgttttc tcactaccta agccttcttg gtataaagaa ctatcgcaaa
      181 gtataatccg ctgagtacaa aagaaagtgt cgtctactat ttctctaagt tgtttacaaa
      241 tgatattact aatagaaaag tgttctccca aaatgtcttc taactgttgt cgatagtata
      301 aatacctaaa gtctgacgga gctactatga acacttcaat tggagatttc cgtttatgct
      361 catttaaaag acttaaacta atggttagtt tacgaataga attctcattc caacgcagaa
      421 ataaattgtt ttgttttcgc cattgttcaa gaatttcatg aacttctaag tacaaagcgc
      481 gttgctcttc atttaacaag taagtcttat caggttgaaa tatctgaata tcagcccaaa
      541 gtgagcggat aaacgggaga agtgctttat caaaaagtat ctgattttct tcagatatat
      601 tcagagataa gataagtttt tgactgagct caatcaccaa agggttatct gctacatggt
      661 tttgataaac aacgttcaag tctttttgta acaattcaaa gttatcgcat agataatttc
      721 tagagttgaa tagaaagtag atataaaggc attcatcttc gtcatgacat aaagagaaag
      781 aaagttgctc taatcctttc tgtattagct gaaacaaggg agtatgtcgg gcaactcttt
      841 tttcttcctg actaaaatat attgggttag tcttagatct ctgcaaaccg atagcgatac
      901 ctaataaaat aaattgtttt tcaactgtag ggtaacttct tgaaaaaaat gtttttctac
      961 ataatcaatc aattgttgac cgctttgaat aattgattta tttagctgga tcaactctgt
     1021 ccgatttgtc tcatagatta tcgttagtaa caaacttcga taatcctttt caggaatttc
     1081 aaaatggcca tcagcattta agtactgcat ttgtttaaaa aaattaatca actcatttcg
     1141 taagttgtaa actttgctta acgaaatgaa ggtagtatca gctatattac tccaattgtg
     1201 ttcccctgat aaataatgtc ctagtacatt gaaaaagtca gagcgctgat agatgagttg
     1261 agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact gtaaagctac ttctagattt
     1321 ctccactgaa ataatttgct gatggagtat gtcggtaatg cattccaagt cgttagtgac
     1381 agtgagtggg caaacgttca attgatctgc tatttcgcga atctgaatgg tccctttttc
     1441 gtgtaattgc tcacaaatgt aaacttgacg aaaaatactt ttctccagat aggtttccaa
     1501 catagtactc ctcctagtca ttttatacat aactattata actctacca
//
LOCUS       DADVXO010000241         1545 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.249, whole genome shotgun sequence.
ACCESSION   DADVXO010000241 DADVXO010000000
VERSION     DADVXO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.249"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..512
                     /locus_tag="KB950_002834"
     CDS             <1..512
                     /locus_tag="KB950_002834"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6858986.1"
                     /translation="YKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPY
                     YAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYI
                     EPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKK
                     KRRRTQKFL"
     gene            640..1323
                     /locus_tag="KB950_002835"
     CDS             640..1323
                     /locus_tag="KB950_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBB6858987.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
BASE COUNT          518 a          246 c          321 g          460 t
ORIGIN      
        1 attacaaaaa aataactttt ataagttcta taacgttgat tatattttta attcttattc
       61 ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt gaggacaaag
      121 aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa aatacaaatg
      181 agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa atctcgacgc
      241 gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct gatacaaaga
      301 cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc atctttcaga
      361 ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag gggttattct
      421 ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta gagttgaaaa
      481 agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa tgtattccag
      541 agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt
      601 agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga aatgatgcat
      661 cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa aaaattaccg
      721 ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt tattccccat
      781 gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg aatcttggag
      841 atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct
      901 catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa
      961 cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga tatcttgccg
     1021 gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa atatattgaa
     1081 tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt agatgacatc
     1141 ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa acgagctatc
     1201 catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt gacatcgaca
     1261 atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac aatcgagttg
     1321 tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca attcgaactt
     1381 gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct aaataaacag
     1441 gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa cagctgaagg
     1501 agaatgtctc gttttaaaag attgatttag tagttgggcc aaaat
//
LOCUS       DADVXO010000242         1531 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.250, whole genome shotgun sequence.
ACCESSION   DADVXO010000242 DADVXO010000000
VERSION     DADVXO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.250"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            52..474
                     /locus_tag="KB950_002836"
     CDS             52..474
                     /locus_tag="KB950_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858988.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            484..687
                     /locus_tag="KB950_002837"
     CDS             484..687
                     /locus_tag="KB950_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858989.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            898..1482
                     /locus_tag="KB950_002838"
     CDS             898..1482
                     /locus_tag="KB950_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6858990.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          544 a          220 c          285 g          482 t
ORIGIN      
        1 aagatttatt aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat
       61 aagtttattt tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt
      121 cctacatttg ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta
      181 actcctttag ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact
      241 gaaaattatt taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg
      301 acaggaacga tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat
      361 tggaaagaaa aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa
      421 tcagctggaa acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt
      481 tttatgccag aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa
      541 attaatttac cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa
      601 aaaattcaaa aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac
      661 caaaaaacat atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc
      721 tttttttcta attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc
      781 tttatttttc aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc
      841 agttaaacta tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg
      901 aaaataggat acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc
      961 ctaaataaat ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat
     1021 cgaccaggat tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg
     1081 cgcttagatc gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat
     1141 gagcgtggtg tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct
     1201 acaggacagc tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc
     1261 ctggaacgat ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt
     1321 cctgaaaaat tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca
     1381 cctattaaga ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg
     1441 gcaaaaatgg aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct
     1501 gaaaattttt gccatttatc tagaaaagtg a
//
LOCUS       DADVXO010000243         1524 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.251, whole genome shotgun sequence.
ACCESSION   DADVXO010000243 DADVXO010000000
VERSION     DADVXO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.251"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            12..1478
                     /locus_tag="KB950_002839"
     CDS             12..1478
                     /locus_tag="KB950_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBB6858991.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          324 g          494 t
ORIGIN      
        1 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
       61 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      121 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      181 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      241 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      301 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      361 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      421 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      481 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      541 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      601 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      661 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      721 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      781 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      841 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
      901 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
      961 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1021 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1081 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1141 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1201 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1261 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1321 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1381 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1441 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1501 ttgcttattt gcagcagttc ctgt
//
LOCUS       DADVXO010000244         1489 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.252, whole genome shotgun sequence.
ACCESSION   DADVXO010000244 DADVXO010000000
VERSION     DADVXO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.252"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(168..1460)
                     /locus_tag="KB950_002840"
     CDS             complement(168..1460)
                     /locus_tag="KB950_002840"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBB6858992.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          404 a          337 c          263 g          485 t
ORIGIN      
        1 gtttttgtca cagtccctta ttctgaataa acagtgttca agagacagcc tatcggcaat
       61 aaggctaaac gtctctttca ctacctcttc aagttattat tatcgtatag ataaacactt
      121 cgactcgctc tggtaagaac gtcctgattc agtcgtcgtt tttatcctta gatttgcgcc
      181 cagacgcttt ctaagatgtt tgtttgtgtt ctatcaggtc cgacagaaaa tgttgagata
      241 cgtacaccaa caagctctga gatacgacgt acatagttac gtgcgttttc tggcagttct
      301 gcaagtgttt tgcatcctgt aatgtcttct gaccagcctg gcaattcttc atagattggt
      361 ttgcagcggt tcaactcttt caagcttgct ggatagtggt agattagttc gccatctaat
      421 tcataagccg tacaaatttt tactgtttcc aatccactta atacatcgat cgagtttaaa
      481 gataggttgg taatacctga aactcgttta gaatggcgca taactacaga atcaaaccag
      541 cctacacgac gcggacgacc agttgtcgta ccgtattctc gtcctacttc gcggatttgt
      601 tctcctgttt catcaaataa ttcagttgga aatggtccat caccgacacg tgacgtatag
      661 gctttgcaga cgccgacaac tttattgatt tttgaaggcc ctacaccgct tccgattgtt
      721 accccaccgg ctactgggtt agaagaagta acaaaaggat aagttccctg gtcgatatcc
      781 agcattacac cttgtgcgcc ttcaaacaac acgcgtttgc ctgcatccaa agcatcattc
      841 aagataacgg aagtatctgt gacgtattgt ttgatttgtt gtccgtattc ataatattct
      901 tcaaaaatat cgtcaaaatc aagtggttct gaatcaaaca tttttacaaa ttgacggttt
      961 ttgtcatcta gattcaaacg aagacgttct tcaaaaattt ctttatctaa taagtctgca
     1021 atacggatac ctacacgagc tgctttatcc atataggcag gtccgattcc tttaatcgtt
     1081 gtaccaatct tattttcgcc cttagcatct tcttgtaatt ggtccaattt gatatgataa
     1141 ggcaagatta cgtgcgcgcg gtcagaaata cgcaaattac ttgtttctac acctttttca
     1201 tgtaaataag ctaattcttt aaccaatgat ttaggattga ctacaacgcc gtttccgatc
     1261 acactgatct tgtctttata aaaaatccca gaaggaatca aatgcagttt ataagtcaca
     1321 ccgttaaact taattgtgtg ccctgcattg tcgccacctt ggtaccgtgc aatcacttca
     1381 gcattttcac tgagaaagtc tgtgattttt cctttccctt catcgcccca ttgcgtacct
     1441 actactacta cagatgacat gtgaacacct cgttctatat tatttaatc
//
LOCUS       DADVXO010000245         1482 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.253, whole genome shotgun sequence.
ACCESSION   DADVXO010000245 DADVXO010000000
VERSION     DADVXO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.253"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1412
                     /locus_tag="KB950_002841"
     CDS             <1..1412
                     /locus_tag="KB950_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6858993.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          585 a          233 c          323 g          341 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat ga
//
LOCUS       DADVXO010000246         1480 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.254, whole genome shotgun sequence.
ACCESSION   DADVXO010000246 DADVXO010000000
VERSION     DADVXO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.254"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            64..972
                     /locus_tag="KB950_002842"
     CDS             64..972
                     /locus_tag="KB950_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBB6858994.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="KB950_002843"
     CDS             972..>1480
                     /locus_tag="KB950_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6858995.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DADVXO010000247         1479 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.255, whole genome shotgun sequence.
ACCESSION   DADVXO010000247 DADVXO010000000
VERSION     DADVXO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.255"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            76..690
                     /locus_tag="KB950_002844"
                     /pseudo
     CDS             76..690
                     /locus_tag="KB950_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1060..>1479)
                     /locus_tag="KB950_002845"
     CDS             complement(1060..>1479)
                     /locus_tag="KB950_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBB6858996.1"
                     /translation="GTDKDFDDNLDIFDPIEFDNDERLLLIYAFMDENSIFSTDNSTY
                     RKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKF
                     KRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          535 a          241 c          216 g          487 t
ORIGIN      
        1 taacattgaa aaatttagag ttctaacgta tgattagggt gtattataaa cgttatagaa
       61 aagtagggaa taaatttgaa aattgcttat attagagttt catccatcga tcaaaatgaa
      121 caacgtcaaa ttgaagaaat gaaaaagttt ggcgctgaaa aaatttttat cgaaaaacaa
      181 tctggagcga cgattagtca tcggccgatt ttccaggaag ctttagattt tgtcagagag
      241 caagatattt ttattgtaga agccattgat cgacttggac gcaattatga cgagattatc
      301 gattcagtga attatctaaa gaagaagaat gtgcgactta ttatcaccag tttaccgatc
      361 atggctgaag caattggaaa tcctctccta gataaattca tcaaagactt aatcatttaa
      421 atctcagcca tgattgctga acaagaacgt actgaatcga aacgacgcca ggcacaagga
      481 attaaaatcg ctaaagcaaa cggcgtctat aaaggtcgtc ctaagttata cagtgcagac
      541 accaaagatc ctcaacgtcg ccttgtctat aggagtatcg ttcaggatct tgagaatggg
      601 gttgctatct caaagattgc aacagactac aatgtgacta ggcaaacaat ttaccgaata
      661 aaaaaagaaa tcgaccaact aattgtatga tttctttttt gaatcacgta attattaatt
      721 agctttgtca tcaatacaga caatcatttt agattcatct agctaagttg attacattta
      781 tgttaaaaag aatttagtta acataaatat aattgatgca taaatcgtcc ttatttaaag
      841 gcatttaaca ttgacaactt agtataatgt atcttatgta aactaataag atttgaattt
      901 tattttatac acactcgata atagatcaat tttatggtcc cccgcattta cgattgcaca
      961 agagaaacat aaagtcgacc tattagcatt tcttttcaat tcaaacatta aaaaagttta
     1021 tacctctgct actaaaaaat gaaattatct atttaaaatt taaaacggta aatctgattc
     1081 atccaaattg gaaaatggat cagcctgtat tggagctttg tatttctcca taataacaga
     1141 tatctttttt tcatttttta aaaaatacct tttaaatttc ttatagtcct ctgaaatcaa
     1201 aatatagcgt gatggatact catcaaaaga agaatcgtat tcttccttta aaattcccga
     1261 tttacttaat ttttctagta catcaaatac tctatcactc aagtaactcc ttaaattgtt
     1321 atcactttcc cattgaacta cttttctata agttgaatta tcagttgaaa agatagaatt
     1381 ttcatccata aacgcatata ttagcagcaa tctttcatca ttgtcaaatt ctattggatc
     1441 aaaaatatct aagttgtcat caaaatcttt atcagttcc
//
LOCUS       DADVXO010000248         1452 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.256, whole genome shotgun sequence.
ACCESSION   DADVXO010000248 DADVXO010000000
VERSION     DADVXO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.256"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..413
                     /locus_tag="KB950_002846"
     CDS             <1..413
                     /locus_tag="KB950_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBB6858997.1"
                     /translation="SPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIE
                     NEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILD
                     IDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            418..1365
                     /locus_tag="KB950_002847"
     CDS             418..1365
                     /locus_tag="KB950_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBB6858998.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          487 a          208 c          290 g          467 t
ORIGIN      
        1 cttcaccttt acgtgaagtt tctaatcttg aagaattaat agatgaacat ctaaaaaatt
       61 tagataaaga cgtctacttt tcagttgttt ctgcaagacg taatccctat tttaatatgg
      121 taaaaagaat tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac
      181 agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa
      241 aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa
      301 ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt
      361 tagaagattc ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg
      421 agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta
      481 ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt
      541 gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa
      601 gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg
      661 gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg
      721 attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca
      781 gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca
      841 ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat
      901 ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat
      961 ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc
     1021 gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt
     1081 tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg
     1141 aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc
     1201 aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc
     1261 catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt
     1321 attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa
     1381 gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa
     1441 tctgggatag aa
//
LOCUS       DADVXO010000249         1423 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.257, whole genome shotgun sequence.
ACCESSION   DADVXO010000249 DADVXO010000000
VERSION     DADVXO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.257"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            107..>1423
                     /locus_tag="KB950_002848"
     CDS             107..>1423
                     /locus_tag="KB950_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6858999.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESR"
BASE COUNT          512 a          196 c          266 g          449 t
ORIGIN      
        1 gatgaaaaaa caagcataat ccatcataaa atcagtatag gtgaaaagat gaactttcat
       61 ctgcatatca agtaattggt atgaggcatt ttttgaggtg taggatatga agttggatta
      121 ccttatagaa gaggaagata gagaaaaact gctgctagct agatatttag aatttgagaa
      181 aaagaattat gtagtgctgc ctaagttata tgaaacatta ggtttgtcta aatttaaaac
      241 aagaaattat atcatagaat taaataaaga atttactgaa ttcaaaacaa agccagagat
      301 ccaaatttca gaagaagcag agatccatgt cgaacgtctg aatttgacgg atatcaaaaa
      361 tttaagaaga atctactttc aacgatcaaa aattgctcga ctgttaacgg agatcgtgga
      421 ttcaaaagta acgatcgaaa aatttgcaga gaatcatttt ttaagcatat caagagccta
      481 tgtaaaaaga agagaattag tctcttttct tgaacagcat caaataaagt taaagaaaaa
      541 catattagtt ggggaagaaa taaacatacg gaacgtatta tttggggtat atttcggggt
      601 ctttaatggt ttttctatgc catttagaga aagcgtcatg aatgaagcaa agagtatcgt
      661 gcaatatttt gaatatctgt tcaatttaaa actgtctgca acaaaacgaa tcaaattcaa
      721 tttgctaacc actattagtt tgttgagggc ctatgggaat caaccaatcg gaaaacgttt
      781 cttttcagat gaatacttta agagctcaga aggaatgaag atcatcaagg atttatcaca
      841 tatttctttt cttcatatcc ctaaagagca gatcattgat gagatttctt atatcatgtt
      901 gtttttgatc acggaagaag aatttgaatt agatggcgca tttggacttg cggaagctca
      961 ttttcatgat atccacatga ttagtcaaaa aatgtcagtg gaattgattg atcaattacc
     1021 attttcgatt gaaatctctg aagaagaaag actatatata cgaaaagaac ttcgtatggg
     1081 tatgatgcat gtcaatcgaa aaaattggat attcgacttt gagatgacta gctttacgtc
     1141 taaaaagcag ctacagtttt tcttcgagac gtatccaata ttcagtacag caatccaaaa
     1201 ggtcatacaa gagaataaac gctatttctt gggaaaagat gaacgtttca tgattcgctt
     1261 attttatgat tatctctttt tattggtcgg tatttgtccg gtatcatatt ttgaagcacc
     1321 gattcatgtc tgtattgatt tttcgcaagg atctgcttat accgattaca ttatttctca
     1381 agtgaaagga tttaaaaatt acaatattgt gattgagagt cga
//
LOCUS       DADVXO010000250         1409 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.258, whole genome shotgun sequence.
ACCESSION   DADVXO010000250 DADVXO010000000
VERSION     DADVXO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.258"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            78..1031
                     /locus_tag="KB950_002849"
     CDS             78..1031
                     /locus_tag="KB950_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6859000.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1003..1278
                     /locus_tag="KB950_002850"
     CDS             1003..1278
                     /locus_tag="KB950_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBB6859001.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          511 a          205 c          256 g          437 t
ORIGIN      
        1 agtatattag atatatcgaa tatattaagt atatgaagta tactaaatat atttagtata
       61 ggaaattgga gggaactatg gaaaacttac ggactgcatt taaaacatca gataaactag
      121 gaaaagttgt aatgcttaca ggtagaaaag gcggcattgg taagactaca gacaatgatt
      181 tattagcgat tgtttctagt caactttttg aaaaagacgt gttgctaatt gattatgacc
      241 aacaaagaaa tactacttcc aacattggat caacgtatca aattactagc tttgatcgat
      301 ccatgtctgc agcaatcaaa aaaggggact gggtttcagg tattactcaa gtaagtccac
      361 atttatatat tatggctggc tctcctggat ctgaagaact taatgaatac ttgtccgaaa
      421 aatatcctga tcgtcgtaag agatcattgg ccttcattaa acctttagaa gaattgagaa
      481 aaaatttcga ttatatcttt atcgactgcc caccatcaac agataatgtg gttcgtgcat
      541 tcttaactgc tgcagattat attattgcaa tgcaagaatt aaaacggtac gcaatggaag
      601 gaacagaaga ttttattaat aaagttttag ttccgattgt cacgaatttt gaagagtctc
      661 atcttcaaat cattggaatt ttgcctgttc ttttctcagt gcgacgttcc tctcaacacg
      721 caaactatca aaaaacaatt gataagtatg gtgaatcaaa catttttaaa agcattgtta
      781 aaggctcgga tcggttagaa atgtatggtg agaatggtat ccaattaaat gattatgtag
      841 atcgaagatg gtgggctatt tttgcagaca tctttacaga attagaagaa agaattgaat
      901 actatgaaca acatggtgat attgaaggat ttgattatac acctaaatat gctgactcaa
      961 tggccaatca tatcctacct cttggaaagg agattaaact aaatggcatt attaccaacg
     1021 aacaaagata agaagaaaat tggtgctgaa gatatttcaa ttaaagatga tattaatgtc
     1081 gagttagggg aacgaaagta cactgcgaaa gaaagaaaac cagttcaagt agatcctccc
     1141 gtactaaaaa ccattcggga tcttagttat gcaaaagata ttcctatgta tgaaatagtc
     1201 aaacttgcaa tggacgccta tgttgatact ttaaacgacg aggaacggat tatatataat
     1261 agacgatcaa aagattaaaa tatagtaagt atactagata tagtaaatat atttagtata
     1321 cttactatat ttttagcttt attaatccta atccttcaaa atatgtgtat tgtttggggg
     1381 tatacaacat tcacaaattt tattgtttc
//
LOCUS       DADVXO010000251         1364 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.259, whole genome shotgun sequence.
ACCESSION   DADVXO010000251 DADVXO010000000
VERSION     DADVXO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.259"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(140..1288)
                     /locus_tag="KB950_002851"
     CDS             complement(140..1288)
                     /locus_tag="KB950_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6859002.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DADVXO010000252         1363 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.260, whole genome shotgun sequence.
ACCESSION   DADVXO010000252 DADVXO010000000
VERSION     DADVXO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.260"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..534)
                     /locus_tag="KB950_002852"
     CDS             complement(<1..534)
                     /locus_tag="KB950_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBB6859003.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDL"
     gene            complement(693..959)
                     /locus_tag="KB950_002853"
     CDS             complement(693..959)
                     /locus_tag="KB950_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859004.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(949..1305)
                     /locus_tag="KB950_002854"
     CDS             complement(949..1305)
                     /locus_tag="KB950_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBB6859005.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          375 a          267 c          167 g          554 t
ORIGIN      
        1 taaatcaaag cgttttgatt ctaaattatt ttgtgctttc tcaattaaat aattcacttt
       61 ttttgtgaga aaccttctga tattgtcttt gctatatgtt ccataaaaac ccttatcttc
      121 taaagagtcc ctgtacttct taacttctat atactcttca gccaaaactt gtggaactgg
      181 ttcatctaag aaaaaaaccc ctacctcgcc tttgctattt ttataaaagt aatcaatttc
      241 tgctatagtt cccgatctat aattatcaac tggagaaccc aacttatggc caaaaatcgc
      301 taataatata tcactatttt ctacaatttg tttattaata acactttgcc cagagctatt
      361 atttatataa gtaggaatcg cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt
      421 attatctgta ttttctatat tccatctata tatggtttct tctacttcat tcctctttgc
      481 tgctatatca cctggtgatg ctataaatac ttttattaga tttatggttt tcatttattg
      541 tacctttctc tttatgtctc tttaaagtaa tttttgtata aaatttattg acttttacac
      601 tataggacaa cttttatcta tttagtacaa aaaggcccac taaactaatt gattagttag
      661 gagcctatta caggtatatt ttcaactcgc ttttaagcaa attctactac cagctttttg
      721 cctaatgccc tagcgatatc atcaagttta tcgaaagata cgttatcccc tctctcaatt
      781 cgagcaatag tagattgagt tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct
      841 gcttcctccc gtgcatgata aagagcaacc gcagtttcga gttttcttcc ttcacggtca
      901 tatgcttctg caaaatcttt gtctttttgc ttttcttcaa tcaatttttt aatcatcatt
      961 tgtgaaccac tcctctctaa gttcttttgc atgtttaagt tcacttattg gtgttttctc
     1021 cgttttttta gtaaatccat gggtaatgac ataccttcca ttgaccacat ggaaatagat
     1081 agctctttgg atgtttgatc ctaccttact tcttaattcg tacaaattag tgtctaattt
     1141 tttcacccat ttcaaccgtt gagctactag catcccattc ttttctgttt cttctattgt
     1201 acgtaaaagc ttcgcactat ctttttttgg taatgtttcc aaccattcta aaaattcatc
     1261 atgaccatta ggtcttttaa atgtttcaaa agatattttt tccataccca attatatcat
     1321 atattgattt tatattatag tatataacat aaaaattcga att
//
LOCUS       DADVXO010000253         1355 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.261, whole genome shotgun sequence.
ACCESSION   DADVXO010000253 DADVXO010000000
VERSION     DADVXO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.261"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1355
                     /locus_tag="KB950_002855"
     rRNA            <1..1355
                     /locus_tag="KB950_002855"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DADVXO010000254         1343 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.262, whole genome shotgun sequence.
ACCESSION   DADVXO010000254 DADVXO010000000
VERSION     DADVXO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.262"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            316..570
                     /locus_tag="KB950_002856"
     CDS             316..570
                     /locus_tag="KB950_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6859006.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="KB950_002857"
     CDS             571..888
                     /locus_tag="KB950_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBB6859007.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          468 a          217 c          264 g          394 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tat
//
LOCUS       DADVXO010000255         1331 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.263, whole genome shotgun sequence.
ACCESSION   DADVXO010000255 DADVXO010000000
VERSION     DADVXO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.263"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            122..>1331
                     /locus_tag="KB950_002858"
     CDS             122..>1331
                     /locus_tag="KB950_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6859008.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
BASE COUNT          415 a          261 c          255 g          400 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta a
//
LOCUS       DADVXO010000256         1261 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.264, whole genome shotgun sequence.
ACCESSION   DADVXO010000256 DADVXO010000000
VERSION     DADVXO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.264"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(315..1205)
                     /locus_tag="KB950_002859"
     CDS             complement(315..1205)
                     /locus_tag="KB950_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBB6859009.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          314 a          280 c          230 g          437 t
ORIGIN      
        1 acaacaaggt ctgatctttg aaaactaaaa aaatatgcgg cgcacagaac gcgccgcata
       61 tcggtctaag ttttattcaa atttgtcttc accaacacga tttgacgaag agccaaaaaa
      121 agttacgaca gttctatgtc gtagctcttt ttttcgaata aacggtgttc aatcagctga
      181 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcac aattaaagga
      241 aaaatgtata tgtggggaaa catagttgat ttttccttaa tagtaacgac taccgtggca
      301 tgtttatctg ctagttattt gatatttcct tcgtgatcac gttctacttc catcattgtg
      361 attggtaaat agcccaattc tttgacaggt ccttcttgga tttcttctgt catcatgtag
      421 tctaagaatt tttggacttc aggggaaggt tttccgttcg tgtacatatg ttcatatgac
      481 cagattcccc agctgttatc tgctacattc tcttcttttg gttctacacc gtcgatactt
      541 agtgcttgtg tggaatcatc gagatatgag aaagctaaat agctgattgc tcctggtgtt
      601 tgacttacca gctgccgaac tgttcctgat gaatcttgtt cttgggactg cacaggggta
      661 gcaccatcca gtccccattt ttcgaatgtc gcgcgggtac cgctgccgtt tgcacggttc
      721 acgacgttga ttttttgatc tttcccgcca acttccttcc agttagtgat ttttcctgta
      781 aagatatcaa tcaattcctg tttggtgata tctttgacat ctgtatcttt attgacgatt
      841 ggtgccatcc cgaccactgc tactttatga tccactaatt ttgaagcatc aactccgtct
      901 ctttcttctg caaatacatc agagtttccg atagtaactg ctccagcttc gacttggctc
      961 aaaccagttc cgcttcctcc tccttgtaca gagatttgat aatttggatt ttcttgtaca
     1021 aagctttctt gtgctgcatc gactaacggc tgtaatgctg tcgaacctac cgcaacgatt
     1081 tttacttgtt catttgagtc acttgaatta ttttgtgtgg cagagccatc tgtatttcct
     1141 gatcctgagc cgcatccggc taaaagaatt cccatcatac ctagagtaaa caatactttt
     1201 ttcattttat tgctcctttc ttatttacaa ctaaagttta tcagtcgact gtaaatccct
     1261 c
//
LOCUS       DADVXO010000257         1257 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.265, whole genome shotgun sequence.
ACCESSION   DADVXO010000257 DADVXO010000000
VERSION     DADVXO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.265"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(569..934)
                     /locus_tag="KB950_002860"
     CDS             complement(569..934)
                     /locus_tag="KB950_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859010.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1010..1255)
                     /locus_tag="KB950_002861"
     CDS             complement(1010..1255)
                     /locus_tag="KB950_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859011.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          427 a          219 c          244 g          367 t
ORIGIN      
        1 aattattaat tttcgtctga aaacaaaaaa agaaagcgtt attattttag tctattttca
       61 gggggggaac cttatgataa aaaaactatt tttactatca atattaacgc tagcaatggc
      121 aggttgttct gactctggct tagcaactga actgatcaca aaatcaactg aagccacgac
      181 agaggagtta aaatgtggaa acgatcacag tgactacaga ggcttaaatc gacgatgcat
      241 gggaatatta ggcagaagca attgtaagca tcgatgtagt aatcgaagaa ggtgtagaag
      301 aagcaacaga aaaatcaaaa gaaagatcaa aagaagcaat ctgtgctgtg catcgtatac
      361 agacgtcgaa gatgcggtac ttttaaacca cgtcaggaga tgttcattta tgtctaggga
      421 agttggcgat gaaccaaaac tagagtacgc ttttagtttt gcacgtgagg atgaagataa
      481 ccctaaaact tacgacattt tagcttgatc taagatggtt aaagcttttt aatgaagtaa
      541 gaggagatag aaggcttttc tagttatatt aggtaagata aattcctttt tgctccaaat
      601 ttgctaaaca ttctgctaga taaacttcat cgtgttccgg ataaatcggc acgttgcgct
      661 ctacagtagg taaatcaaaa aatgcttcgc atgatttgcc acgatacata ggatttttcc
      721 actctggtgc tgggctgtaa ccacgttttt ccatttcatc catgacaagc tgatgatatt
      781 ggaataatct ataaggagaa tagtgaaaaa cataatccac agtagcatgt ttctttcccc
      841 agccatttcc tcttaatgca cagcattccc gatgttgtcc taaaagctgt tgacgaggaa
      901 gctttgaaat caatttttca tgccataatc gcatagaaat gtctccttta tatatagatt
      961 gttaacctta gacgttaaat gaaaaaacca gcaccaaggc tggaaaaaat cacttcagtt
     1021 tgcttgctgt ttctgagcga ccaattaggt aatcaacgga aatatcgtaa aagtcagcga
     1081 tatagattaa ttcaatgatg cttggaattt cttctccttc ttcgtactta cgatatttga
     1141 ataaaggaat acttaacgcg ctagaaacag ctacttgaga taaagatcgg ccagttctta
     1201 agaaattcag acgacaacgt aaagtatcca gtaaaatagc ttcttcgata ctcattt
//
LOCUS       DADVXO010000258         1243 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.266, whole genome shotgun sequence.
ACCESSION   DADVXO010000258 DADVXO010000000
VERSION     DADVXO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.266"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..997
                     /locus_tag="KB950_002862"
     CDS             <1..997
                     /locus_tag="KB950_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6859012.1"
                     /translation="KTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQQE
                     LSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQ
                     NVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVR
                     FGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGET
                     YVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSY
                     ENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1009..>1243
                     /locus_tag="KB950_002863"
     CDS             1009..>1243
                     /locus_tag="KB950_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HBB6859013.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELS"
BASE COUNT          492 a          163 c          232 g          356 t
ORIGIN      
        1 aaaaacaata ttagtaactg gcggaactgg ttccattgga agtgaaattg ttcgtcagct
       61 tttaaaatat gaaccaaaag ctatcagaat ttatagtaga ggtgaagaaa aacaatttta
      121 tatgcaacaa gagctctcaa atcacactaa tttgagattt ttaattggtg atatcagaga
      181 ctctcaaagg attgattatg catgtcgtga tgtggatatt atttttcatg ctgccgcaat
      241 gaaacacgtt ccagcaagtg aatataatcc tatggaagct gtaaaaacaa atgtgatggg
      301 aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg taggtatcag
      361 tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat tgagtgaaaa
      421 attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt cttgtgtaag
      481 gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta aacaaataaa
      541 aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga tgtcaatacc
      601 ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag aaacatatgt
      661 cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa aaatatataa
      721 tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca ggccaggaga
      781 aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg aaaatgataa
      841 aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag attatgaaaa
      901 aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac aggaatttat
      961 tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat gaaatcagaa
     1021 gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt agctttagaa
     1081 agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac atttaaaacg
     1141 aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa tcttaaagag
     1201 gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctc
//
LOCUS       DADVXO010000259         1234 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.267, whole genome shotgun sequence.
ACCESSION   DADVXO010000259 DADVXO010000000
VERSION     DADVXO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.267"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(204..353)
                     /locus_tag="KB950_002864"
     CDS             complement(204..353)
                     /locus_tag="KB950_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859014.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            815..1027
                     /locus_tag="KB950_002865"
     CDS             815..1027
                     /locus_tag="KB950_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859015.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          458 a          186 c          206 g          384 t
ORIGIN      
        1 tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt
       61 ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag
      121 acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt
      181 cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg
      241 attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta
      301 gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc
      361 atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc
      421 aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta
      481 ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg
      541 ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa taacttagca
      601 atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg
      661 tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa aataaaaaat
      721 aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa aataaagaaa
      781 aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag taggaagatc
      841 ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg aacgtacata
      901 tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc aacttatcaa
      961 tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg ctgaattggt
     1021 ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa taaagacaga
     1081 agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat tattcttgca
     1141 gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga atcaagagaa
     1201 agttaagcac acattatgag cgtcaaacaa aaaa
//
LOCUS       DADVXO010000260         1219 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.268, whole genome shotgun sequence.
ACCESSION   DADVXO010000260 DADVXO010000000
VERSION     DADVXO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.268"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..460
                     /locus_tag="KB950_002866"
     CDS             <1..460
                     /locus_tag="KB950_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBB6859016.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="KB950_002867"
     CDS             469..696
                     /locus_tag="KB950_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859017.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          377 a          195 c          204 g          443 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     1081 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     1141 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     1201 cattattttg ataataatg
//
LOCUS       DADVXO010000261         1202 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.269, whole genome shotgun sequence.
ACCESSION   DADVXO010000261 DADVXO010000000
VERSION     DADVXO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.269"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..597)
                     /locus_tag="KB950_002868"
     CDS             complement(<1..597)
                     /locus_tag="KB950_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6859018.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYR"
     gene            complement(587..>1202)
                     /locus_tag="KB950_002869"
     CDS             complement(587..>1202)
                     /locus_tag="KB950_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBB6859019.1"
                     /translation="NIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLRE
                     VNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVG
                     KNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEI
                     HLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT          379 a          226 c          144 g          453 t
ORIGIN      
        1 tcgataagcc tcttctaatt tagaatctat aaataattct ctgtccaacg atttcaaatc
       61 atatttatcc ttattaatat aaaaagaagc atctccagaa ccactcaatt taataagcgg
      121 ttttgaagaa tacttctctg ggttataaat ttcaaaagtt tctgtttgtc gaatagtaaa
      181 tctaccattg taattttcct taaatggtcg aatactcact gtaaattcaa aagggacaat
      241 atttcctgtt tttcttgttc ctttaaattc aggtccttct attactacag cttgataaat
      301 atattgttca tcatagtaaa gaataaaatc actataagac gacatatcta accattcagt
      361 gattcgatct tcccactcct gcaccatatc aattgaaggt gctttatagt aacactcaat
      421 ctttctagtc acatttttgt aatagccttt atcaataatg atagaatcat ttccttctcg
      481 ctctctcaat tcaattactc gactagctga aacagaagca ggcctgtttt gaatatacac
      541 attaaattca gaagaataat gttgattaat aaaaaattgt cctctcttaa gttgcatata
      601 atgttcctcc tactgcacca gcatctcgtt tcatttgtct tgtcaattct gttttaattt
      661 ttgtagtaat ctttcgaacc attgaatcag gtaaatctcc ataaacattt aaatgtaaat
      721 gaatttcttt tgtttcacta gcagatatat tttgctttgt ttgtgtaatt ggtaggtgac
      781 ttattccatt tactacaggt tgaacagacg gtgtttttac taaatttgtc atggtttgat
      841 ctaacttact ttgctcttta tcaataccaa cgataatacc ttgaacaata tttttaccaa
      901 ccatatcacg catccatctt gatggagaat gaatatctaa agctccttta atccaatttt
      961 tgatattacc tgcaatacct ttgatagtat ctttcaaagc attccacttt tcttttacac
     1021 catttataag tccatcaatg atgttcttgc caatttcaaa taaattgact tcccttaacg
     1081 aattaaagat ttcttttacc cgattaatag catttgaaac accatttttt agatttgtcc
     1141 atgcattttc agcagaatta acaattccag taacaatatt ccagaaagag tccttaatgt
     1201 ta
//
LOCUS       DADVXO010000262         1189 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.270, whole genome shotgun sequence.
ACCESSION   DADVXO010000262 DADVXO010000000
VERSION     DADVXO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.270"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            129..1169
                     /locus_tag="KB950_002870"
     CDS             129..1169
                     /locus_tag="KB950_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBB6859020.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          482 a          173 c          199 g          335 t
ORIGIN      
        1 ctgattttag gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat
       61 ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag
      121 gatctacaat gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt
      181 atcaatttcc aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta
      241 agattgccta tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg
      301 atgaagaagg taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact
      361 gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac
      421 aaaagcagat gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag
      481 cagaattaaa agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata
      541 gggaaaacgt tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag
      601 acgctgaatc ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata
      661 aatcagaggg tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac
      721 acaatataga cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga
      781 caattatgac aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa
      841 aaacattttc ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa
      901 agaaaaaagc tgaaatagaa agtgatattt cgatagtttt tgaagaactc gatagttatt
      961 atgtcaatgc agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa
     1021 ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg
     1081 aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt gaattatgca agctccccaa
     1141 gcactattac gaaagactgg ttagagtgaa actttactga caaagtgat
//
LOCUS       DADVXO010000263         1158 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.271, whole genome shotgun sequence.
ACCESSION   DADVXO010000263 DADVXO010000000
VERSION     DADVXO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.271"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            15..275
                     /locus_tag="KB950_002871"
     CDS             15..275
                     /locus_tag="KB950_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859021.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(637..876)
                     /locus_tag="KB950_002872"
     CDS             complement(637..876)
                     /locus_tag="KB950_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859022.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          407 a          163 c          217 g          371 t
ORIGIN      
        1 aggatggatg aaatatgaat aacgaaacta tcattttagg aaaaaaatat atttgccaac
       61 ctattggctt gaaacaaaaa gtggttggag aagtaatcag taaattagaa aattgtgttg
      121 tgatctgcgt agacgaatat catgacgtcg atcgcaatga aatattagaa aaatgcggaa
      181 aagtagtggc aaaatatact cacgtttatg agttggaaac aacagaatat ctatataact
      241 atagcaacaa atatgaacat actttagttt cataaagatt taaataaagt gccttcaaaa
      301 gcgagaagac caagcttttg aaggcacttt atttagttgg cagtgattgt tcatgatttt
      361 tgatttattc aaaaggttcg cctagagaat ctgtctctgt aacgaggcct ggtggagaaa
      421 ctcgataaaa acctatactg ccagaactgt cctattttcg gatagacaaa ctagtagttt
      481 taaaaagatc atccgatcca atcatttcaa aaaatgcaaa actgatatca ggatcattta
      541 gctcaggctt gttatcgtaa agcatttgta aaggttgcat ttagttagga gtaaacgcga
      601 aactatcatc attatttgct gaagaagatg aagaagctaa ttcagagttt gtttgaaata
      661 aaggagacgt gctgacagtt tctttaccct gttttttgca gaggtagaaa cttttatatc
      721 ggaagtttct accggagaat ttttctcatt tgtagaaatt gttgaatcga tactattcga
      781 aatttgcgta tctgaaggta tgttctttga tgcagaagtc ccacaagcag taagtaacaa
      841 taaatttgat aaactgataa acagaattct tttcattatg tacacactcc ttatagtatc
      901 gtttagcata cacctatgct cagtattagg caaaatcaat gcaaaactat caagtctttt
      961 atagtatcta ttagtcttag taatacatat atggagaggg tgatatccca gttcaaacca
     1021 gatacattgg ttaccatctg tattaacgat tatgcctcta aacgcttttt ttatgaaaaa
     1081 atttataagt gaaaacgtag gtatgatatt atctagaggt aatttttttg ggtatttaaa
     1141 tagttgctag gaggatag
//
LOCUS       DADVXO010000264         1153 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.272, whole genome shotgun sequence.
ACCESSION   DADVXO010000264 DADVXO010000000
VERSION     DADVXO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.272"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..606
                     /locus_tag="KB950_002873"
     CDS             <1..606
                     /locus_tag="KB950_002873"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6859023.1"
                     /translation="VYVDFGVNVGYELSGNHFAIVLNNKDNSKNGVVCVVPISSKKKS
                     NYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDESDLNSFIKDIEAKGE
                     KLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNCYKKYTQESYVMPLN
                     IQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(931..1077)
                     /locus_tag="KB950_002874"
                     /pseudo
     CDS             complement(931..1077)
                     /locus_tag="KB950_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          393 a          163 c          224 g          373 t
ORIGIN      
        1 gtatatgttg attttggggt gaacgtgggt tatgagttat ctggtaatca ttttgctatt
       61 gttttaaata ataaagataa ttcgaaaaat ggtgttgtat gtgtagtacc aatctcttca
      121 aaaaagaaaa gtaattatgt atctgtagga aaaatattag aagttgctag tattaaacaa
      181 tttataagcc aagctgataa attaaaagag aaacttagga taattatttt actttcatta
      241 aatagaggca tcattgatga aagcgatttg aatagtttta ttaaagatat tgaagctaaa
      301 ggtgaaaagc tttcagtgga agaagtagaa aaacgagctg taaaacataa tttggtttgc
      361 gagacaaggg aacaagtaat taaagaaata aaatcattat ctgaagatat ggtaaaatat
      421 cagaaggtgt ttaattgcta caagaaatat acacaagaat cctatgtaat gccacttaat
      481 atccaaacga ttagtaaaaa tagaattaga agaattaaca agtttgatcc ttctggaaat
      541 atgaaggcac ctggaaatgt cctcaatgaa gtagatgaaa gtttaaaaaa atattttaca
      601 aagtaaacga tatttttgtt gacatttttc tgttcgttag ttaccatgta gttatagaag
      661 gacgtaagcg cctattttta atgtacacat cgggacgtat gcgcccacat agaaacatag
      721 ttgagagaca tctatatggt gtctcttttt ttattaatat gcaaaaaagt ctagtttttt
      781 caataatatt tatgtaataa taaattagcc accacaacaa agaatgaaat ccattattat
      841 ctagtctagt ccattctttt tgtttgcagt atttgtgatg gctttccgta cccttagctc
      901 agttggtcag agcagacggc tcataaccgt ccggtcgtac ttctcgtaat ccaggaaggc
      961 cgtcagctcc gtccgcagta gcgtgtttac ggccagctcc agatgggagc ggaataactc
     1021 gttcaaatca cctttggtgg ctagagtctg caggagttct gtaataaaat cgttcatagg
     1081 gaagtcctct tttctgtgaa tgggtgtggt aactttattc tacagaaggg acttcctttt
     1141 ttctatttac aca
//
LOCUS       DADVXO010000265         1150 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.273, whole genome shotgun sequence.
ACCESSION   DADVXO010000265 DADVXO010000000
VERSION     DADVXO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.273"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            144..959
                     /locus_tag="KB950_002875"
     CDS             144..959
                     /locus_tag="KB950_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859024.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          435 a          167 c          193 g          355 t
ORIGIN      
        1 tatttgtaag tgactagaga attaaataaa aaataattac tatctagtac gaaattaatt
       61 acataggata atatattaag gtgctggact acaagtaaaa aatactgtat gatcatgaca
      121 gattcaagaa aggagcaata agattggtta agatagcagg actttctaaa ggtggaaaaa
      181 ccgtttcaca actttataaa caaaactaca gagactggag acagttcaga aaaaacatta
      241 aggattctta ctttattcta ccaacagaac ttgaaaaata tttaccgaat aacaaagcta
      301 ttaatttata tctgtattac tgttttaagg caaagaatga aggcggggat tcctggcatt
      361 cggtcaatac catagcagag tctctaggag tgacaactaa aactataaac acctggaata
      421 aaaccttagt tgatttaggt atgattgcac gtatagacga taaccatctc agtaaatcga
      481 caattttact tcctctctct agttactata aattagccct tgatagttct ttaaaggatg
      541 ttaccgagag cactgtccat gaaattgatg gcaacttggt tgcagtttat catttgtttg
      601 aatggagaaa aaatgaggct gatgaaaatt ataacgttcc ttataacaca ctctgttgtg
      661 tttttaaaag aaaaacacaa aaagatacta tctataaatt tatcttggtc caagatgaga
      721 aaataagtaa tttcgaatta agcactcctt ctagtaagat ttatgacgat gcatatagat
      781 ttgaatgtca agatctagaa gccctccttt ccgcaaataa tctaaacaat atagaacatg
      841 aaaactttgt tgtaaacact agatttaatt tagtttctga aaaggactca gatttgttag
      901 atttgctgat agagcttact aaaaatctag ataagaaaga aaacattact gtactataac
      961 tagcatcggt ccatcattaa gtttggacta ttagaacact tgctattaca gaaaaaggta
     1021 tgctagccac gcttactatt tattggtatc aaacaagtat cttaatttga aaggaagatg
     1081 atttatggct aatttatata tagagaacaa aggagacttg aatgccatta ttactttaat
     1141 cgtagttatg
//
LOCUS       DADVXO010000266         1149 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.274, whole genome shotgun sequence.
ACCESSION   DADVXO010000266 DADVXO010000000
VERSION     DADVXO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.274"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            34..906
                     /locus_tag="KB950_002876"
     CDS             34..906
                     /locus_tag="KB950_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6859025.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          333 a          220 c          279 g          317 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaat
//
LOCUS       DADVXO010000267         1147 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.275, whole genome shotgun sequence.
ACCESSION   DADVXO010000267 DADVXO010000000
VERSION     DADVXO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.275"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..969
                     /locus_tag="KB950_002877"
     CDS             <1..969
                     /locus_tag="KB950_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6859026.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          388 a          200 c          229 g          330 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgt
//
LOCUS       DADVXO010000268         1096 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.276, whole genome shotgun sequence.
ACCESSION   DADVXO010000268 DADVXO010000000
VERSION     DADVXO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.276"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..378
                     /locus_tag="KB950_002878"
     CDS             <1..378
                     /locus_tag="KB950_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6859027.1"
                     /translation="ILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVY
                     SFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLIL
                     FFLYPLSKDKVLENCELLKQKHK"
     gene            388..>1096
                     /locus_tag="KB950_002879"
     CDS             388..>1096
                     /locus_tag="KB950_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6859028.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTD"
BASE COUNT          397 a          122 c          218 g          359 t
ORIGIN      
        1 attcttagtt cggttggcta tatgggtttg ggtgtgttta atactgttat ttgggcaaat
       61 attactgatg ttattgatga tcaagaagta aagtctgatc aaagagaaga tgggaccgta
      121 tatgcggtat attcttttgc tagaaaaata ggtcaagctc tagctggagg cgtcggaggt
      181 tggactcttt ctattatagg ctatgactct ttagcaaatg tacaaacggg agatgtttta
      241 gaaaagttat ataacgcttc aacattaatt ccatcaattt gttttctgat tgtaggatta
      301 attttatttt tcctttatcc attatctaaa gataaagtct tagaaaattg cgagctttta
      361 aaacaaaaac ataaatagga gagaaatatg aataattgga tattttgtat tgattcagat
      421 ggctgtgtta tggatacgat gacttataaa cacaatctct ttttcggacc aattgctgca
      481 gagatttttg atgttcatca tagagaaaaa ttcctacagg attggaataa tattaattta
      541 tattcagaga ctcgtggaat aaacagattt ttaggtttag ttaaaacatt agaaaaaaat
      601 gatgtaacat cgattaatcg attaaagcac tgggtatctc taacaaaaga attgtctaat
      661 ccatctttga agagggaaat agaatattct cagtcagagg atttattaaa agctttaaaa
      721 tggtcggaag aagtcaatca acagattagt caagcaaaag gattagataa acccttttta
      781 agtgctagag atactataaa agctctgaaa aaatatggga agataattat tgtaagctca
      841 gctaataagg aagctgttca agaagaatgg gaaagacatg aattattatc attagttgat
      901 gaactgtgtt gtcaagataa aggaaaaaaa gaagatatta ttcgaagtgt aattgaaaat
      961 ggttgtgatt tggataagat attaatgatt ggtgattctc ctggtgatct agaagctgca
     1021 aataaaaata aagttttctt ttatcctatt ttggtcaata aagaaaaaga atcatgggaa
     1081 aacttaagaa ctgatg
//
LOCUS       DADVXO010000269         1045 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.277, whole genome shotgun sequence.
ACCESSION   DADVXO010000269 DADVXO010000000
VERSION     DADVXO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.277"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="KB950_002880"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="KB950_002880"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBB6859029.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DADVXO010000270         1035 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.278, whole genome shotgun sequence.
ACCESSION   DADVXO010000270 DADVXO010000000
VERSION     DADVXO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.278"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>1035)
                     /locus_tag="KB950_002881"
     rRNA            complement(<1..>1035)
                     /locus_tag="KB950_002881"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DADVXO010000271         1022 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.279, whole genome shotgun sequence.
ACCESSION   DADVXO010000271 DADVXO010000000
VERSION     DADVXO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.279"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            48..458
                     /gene="spx"
                     /locus_tag="KB950_002882"
     CDS             48..458
                     /gene="spx"
                     /locus_tag="KB950_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBB6859030.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            472..828
                     /locus_tag="KB950_002883"
     CDS             472..828
                     /locus_tag="KB950_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBB6859031.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          355 a          155 c          200 g          312 t
ORIGIN      
        1 aaagaaatta ctgtagaatt ggattataat taaaaaggag tgagaaaatg ataaaagtat
       61 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
      121 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
      181 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
      241 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
      301 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
      361 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
      421 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
      481 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
      541 catcaattgc cttatcaatt tgttgactta gaagaacgcg atttaagtac agaagaagca
      601 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
      661 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
      721 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
      781 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
      841 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
      901 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
      961 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     1021 ca
//
LOCUS       DADVXO010000272         1002 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.280, whole genome shotgun sequence.
ACCESSION   DADVXO010000272 DADVXO010000000
VERSION     DADVXO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.280"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            370..1002
                     /locus_tag="KB950_002884"
                     /pseudo
     CDS             370..1002
                     /locus_tag="KB950_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cc
//
LOCUS       DADVXO010000273          995 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.281, whole genome shotgun sequence.
ACCESSION   DADVXO010000273 DADVXO010000000
VERSION     DADVXO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.281"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            83..622
                     /locus_tag="KB950_002885"
     CDS             83..622
                     /locus_tag="KB950_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859032.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            619..957
                     /locus_tag="KB950_002886"
     CDS             619..957
                     /locus_tag="KB950_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBB6859033.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT          360 a          130 c          189 g          316 t
ORIGIN      
        1 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
       61 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
      121 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
      181 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
      241 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
      301 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
      361 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
      421 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
      481 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
      541 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
      601 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
      661 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
      721 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
      781 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
      841 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
      901 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
      961 gaatatgcac agttgagaac ggaatttgac attta
//
LOCUS       DADVXO010000274          986 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.282, whole genome shotgun sequence.
ACCESSION   DADVXO010000274 DADVXO010000000
VERSION     DADVXO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.282"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>986
                     /locus_tag="KB950_002887"
     CDS             <1..>986
                     /locus_tag="KB950_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBB6859034.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DADVXO010000275          972 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.283, whole genome shotgun sequence.
ACCESSION   DADVXO010000275 DADVXO010000000
VERSION     DADVXO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.283"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            100..>972
                     /locus_tag="KB950_002888"
     CDS             100..>972
                     /locus_tag="KB950_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6859035.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNN"
BASE COUNT          340 a          169 c          179 g          284 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      301 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      361 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      421 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      481 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      541 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      601 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      661 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      721 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      781 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      841 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      901 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgccgcatct
      961 aaacgaaata at
//
LOCUS       DADVXO010000276          943 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.284, whole genome shotgun sequence.
ACCESSION   DADVXO010000276 DADVXO010000000
VERSION     DADVXO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.284"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>943
                     /locus_tag="KB950_002889"
     rRNA            <1..>943
                     /locus_tag="KB950_002889"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DADVXO010000277          933 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.285, whole genome shotgun sequence.
ACCESSION   DADVXO010000277 DADVXO010000000
VERSION     DADVXO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.285"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            3..323
                     /locus_tag="KB950_002890"
     CDS             3..323
                     /locus_tag="KB950_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859036.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            341..772
                     /locus_tag="KB950_002891"
     CDS             341..772
                     /locus_tag="KB950_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBB6859037.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          350 a          136 c          163 g          284 t
ORIGIN      
        1 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
       61 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
      121 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
      181 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
      241 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
      301 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
      361 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
      421 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
      481 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
      541 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
      601 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
      661 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
      721 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
      781 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
      841 ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa
      901 aatggaaaaa gagattttaa taaaatctcc agg
//
LOCUS       DADVXO010000278          921 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.286, whole genome shotgun sequence.
ACCESSION   DADVXO010000278 DADVXO010000000
VERSION     DADVXO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.286"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            522..908
                     /locus_tag="KB950_002892"
     CDS             522..908
                     /locus_tag="KB950_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859038.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          339 a          112 c          166 g          304 t
ORIGIN      
        1 tatggtttgc tggtaaaaaa tatattacta ctttcaaaag attatccgag ttaatgcagc
       61 agactgttaa aagtgctatg cctgaaggat acaagtttaa ttttgttcaa tcttcagtat
      121 ctactgaaag tatctatttt tcaattcttt ctgagagtag gctttttcat tttagaataa
      181 gttctcatgt aaagaaaact gatgttgata tatatacgtt ccgtacaaat aattataaaa
      241 atttaaatga tttgagtaga agtatcagtg aatatctcaa ggggaatagc tatctcaaga
      301 ttcgattttc acatatatta attttactac catatttgaa tgagaatttc accctaattc
      361 aagtagataa gtctttcatt gcctcaggta atcaaggaaa tatgaatatt acaagtaaca
      421 aaacgtcacg tgtgctattg gcgttgatgg gttacggaat tctttataga gatgaccaaa
      481 atggccagat tctaataaat cgggctgggc gttcattatt aatgaaatat agaaaattta
      541 taccggaaga ttttcaaaac ttacagttag aaaacatgcg tttagaagat ttatttagat
      601 tagcagaaga tgataagtat agatttgaag ttttgaaaat tcaaaagaat gaactattga
      661 ataagttata tcctgagaat gcgataaaag cacattttgg tgatgctaat catatgtata
      721 gttggttcat tcctaaaaga tttagaggga aaatcaatgc tggagatcaa gtgaaagtac
      781 tcaatcgaaa tagtacagat gtcgtaatga taacagatat ctatgtagaa gatgaagata
      841 taatcaaata tatgaagcct gtgataaaac ttgttaataa aaataaagga acccgtggtg
      901 ctagttaatt ttttcctaga g
//
LOCUS       DADVXO010000279          919 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.287, whole genome shotgun sequence.
ACCESSION   DADVXO010000279 DADVXO010000000
VERSION     DADVXO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.287"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..731
                     /locus_tag="KB950_002893"
     CDS             <1..731
                     /locus_tag="KB950_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBB6859039.1"
                     /translation="TIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMV
                     FNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELA
                     VSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFS
                     VSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRV
                     FKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          343 a          104 c          162 g          310 t
ORIGIN      
        1 aaactatcga gcaaatcaaa atagataatc tgttatcaca aaattacgat ccagaaatat
       61 atctgtttct ttatgaaaat aaaatactgg aatatgttaa aaatggagat attcgaaatt
      121 tagaaaacat ggtttttaat ctgagtaatg gaataattcc aagtgttagt ggagatacga
      181 ttcggtctga aaaaaattat tctatcattg tttttgaaaa gttagcacaa acgtctataa
      241 cattaggaat ggatataata gaagcgtatc aaagtagaga tgctttgata caagaaaacg
      301 aattagctgt atcgttacct gaggtcttaa aagtgaggga ttctgggatt gtttattata
      361 ctaaggaaat cggaaaaact aagatagagc atctttctcc cttaatttct tctgtcgttc
      421 aattcatcgg cttaaatatc tataaaagaa ttactgtaaa ggaaatagct aattattttt
      481 ctgtaagtga aactaaatta agagaaagct ttaagaatga aatgcatatt acgatttata
      541 attatatctc taagcgaaaa atttctacgg ctaaaataat gttgaaatca aaccatacta
      601 tcagtgaagt ttctttaggt ttaggatttt cagattcttc acacttttcg agagtattca
      661 aaaagtacgc aggtgtatct cctaagcaat atcaattagg tttagtggac aatttcaaca
      721 acattagctg agaggtttta ttttattatg agactatatc atttacgtag tttggaggag
      781 ttgaggaaat tggattttaa caaaaaattt ttttgggaaa tttctgattt gagttgtttc
      841 gtagaatctg agcgtctaag taatggtaat aatagtgtgt taaatgaata tgacaaagta
      901 gtaattaata aagtaaaaa
//
LOCUS       DADVXO010000280          917 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.288, whole genome shotgun sequence.
ACCESSION   DADVXO010000280 DADVXO010000000
VERSION     DADVXO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.288"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>917
                     /locus_tag="KB950_002894"
     CDS             <1..>917
                     /locus_tag="KB950_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6859040.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLP"
BASE COUNT          316 a          176 c          166 g          259 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcct
//
LOCUS       DADVXO010000281          911 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.289, whole genome shotgun sequence.
ACCESSION   DADVXO010000281 DADVXO010000000
VERSION     DADVXO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.289"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            96..>911
                     /locus_tag="KB950_002895"
     CDS             96..>911
                     /locus_tag="KB950_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBB6859041.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSD"
BASE COUNT          314 a          135 c          159 g          303 t
ORIGIN      
        1 attttgtgta aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag
       61 ttaccacaac cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta
      121 tggaaacact aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag
      181 ctgttaattc attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc
      241 gtgctgggtt caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag
      301 agtatggaga attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa
      361 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc
      421 aaaaaggaat tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact
      481 atacgccaca aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta
      541 aagaaaggtc cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt
      601 taaaaagaca aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg
      661 gaaccaaaga ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag
      721 agattcttca agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg
      781 gtttaagtgg cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      841 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      901 tctgtagcga t
//
LOCUS       DADVXO010000282          895 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.290, whole genome shotgun sequence.
ACCESSION   DADVXO010000282 DADVXO010000000
VERSION     DADVXO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.290"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(570..863)
                     /locus_tag="KB950_002896"
     CDS             complement(570..863)
                     /locus_tag="KB950_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBB6859042.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          265 a          161 c          129 g          340 t
ORIGIN      
        1 tatctactat ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg
       61 tcgactgcag tgttgtgtcc ctcatcacga ataaacggtg ttcaattagc tgatcttgta
      121 ttcacttaaa aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc
      181 aggacaaaat actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa
      241 cgaaatcttg ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt
      301 ttggtattta ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg
      361 attttttcat gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc
      421 gctcattagc ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat
      481 agatacagat gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa
      541 cttttttcaa tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc
      601 cgaccgtctg aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca
      661 taatcgccac tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc
      721 actccatggt caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa
      781 cttggtactt gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct
      841 tgctccgtat aagtataaac catcttcttt ctccttttga ttctaagaat actcc
//
LOCUS       DADVXO010000283          894 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.291, whole genome shotgun sequence.
ACCESSION   DADVXO010000283 DADVXO010000000
VERSION     DADVXO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.291"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..206)
                     /locus_tag="KB950_002897"
     CDS             complement(<1..206)
                     /locus_tag="KB950_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859043.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSF"
     gene            complement(208..>894)
                     /locus_tag="KB950_002898"
     CDS             complement(208..>894)
                     /locus_tag="KB950_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBB6859044.1"
                     /translation="KFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPV
                     SIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGY
                     GETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYV
                     LNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGS
                     ISYLYLKKEN"
BASE COUNT          331 a          159 c          104 g          300 t
ORIGIN      
        1 ttaaaggaag aaccgatttt ccggtgattc acaaaaacaa catcattaaa tagccctaat
       61 tcttttatac gctcactata tcttctagcg ttttcaaatt cagaataaat aaataaatct
      121 ccttttgatt ctcggtaaaa attttgataa acatttaata tattaatcaa atgtaaagga
      181 gtccaagcaa tgaaagctgt cttcatatta attctccttt tttaaataca agtaagaaat
      241 gcttcccaaa taataccaga aaacaattga aatgacatcg cgcttattga aaagtacatg
      301 agcctccacc atttcatcta taaaaataga tagtataaaa attaaaacaa taccaagaaa
      361 cgcataattc actttacttc taaaaaatgt tttgagtaaa ctaaagatat ttaacacata
      421 gaaagcatag atacatataa aagcaattat accacttgat acaagtactt gaagaaatcc
      481 attatgcatg tttccatgtg ctctttttag ttcattcaca tctgtagtat caagatctac
      541 ttttttcaaa aattttgcat cgctattccc atagaaatcg gtctctccat aaccaaataa
      601 caaagttggt cttataactt taagcgcagc tttccaaatc gtaaaacgac cagctgaaat
      661 ttcggagtta tcactagaat gctgtaatat tacttctttt tctggaagtg gagttttatc
      721 tgattcattt gactctataa cttctacttt tctatcaatc gatacaggaa ttgttgctaa
      781 aaaatcctta gcataattag taccaaaaga aaaatatacc aatagaacta ataatatgct
      841 taccatccat ttgattttta caggtagtac ttctctaatg ctctttctaa attt
//
LOCUS       DADVXO010000284          889 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.292, whole genome shotgun sequence.
ACCESSION   DADVXO010000284 DADVXO010000000
VERSION     DADVXO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.292"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(76..148)
                     /locus_tag="KB950_002899"
     tRNA            complement(76..148)
                     /locus_tag="KB950_002899"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:His,seq:gtg)
     gene            complement(151..224)
                     /locus_tag="KB950_002900"
     tRNA            complement(151..224)
                     /locus_tag="KB950_002900"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Trp,seq:cca)
     gene            complement(231..311)
                     /locus_tag="KB950_002901"
     tRNA            complement(231..311)
                     /locus_tag="KB950_002901"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(275..277),aa:Tyr,seq:gta)
     gene            complement(313..388)
                     /locus_tag="KB950_002902"
     tRNA            complement(313..388)
                     /locus_tag="KB950_002902"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(353..355),aa:Phe,seq:gaa)
     gene            complement(424..496)
                     /locus_tag="KB950_002903"
     tRNA            complement(424..496)
                     /locus_tag="KB950_002903"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(461..463),aa:Asp,seq:gtc)
     gene            complement(506..578)
                     /locus_tag="KB950_002904"
     tRNA            complement(506..578)
                     /locus_tag="KB950_002904"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(543..545),aa:Val,seq:tac)
     gene            complement(594..665)
                     /locus_tag="KB950_002905"
     tRNA            complement(594..665)
                     /locus_tag="KB950_002905"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(630..632),aa:Glu,seq:ttc)
     gene            complement(680..769)
                     /locus_tag="KB950_002906"
     tRNA            complement(680..769)
                     /locus_tag="KB950_002906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(733..735),aa:Ser,seq:gga)
     gene            complement(809..882)
                     /locus_tag="KB950_002907"
     tRNA            complement(809..882)
                     /locus_tag="KB950_002907"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(846..848),aa:Asn,seq:gtt)
BASE COUNT          248 a          244 c          204 g          193 t
ORIGIN      
        1 gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac
       61 cccaagaata aaaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc
      121 ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc agcaggaatt gaacccacac
      181 taacggtttt ggagaccgct gttctacctt taaactatgc ccctataaaa tggaggagag
      241 tggattcgaa ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc
      301 gctactcctc catggtggcg cgggacagaa tcgaactgcc gacacacgga gcttcaatcc
      361 gttgctctac caactgagct accgcgccat attttaaaat tattaattga ataacaagaa
      421 cgacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc
      481 cctacaccac ggaaccagtt cattatggag gttaacggga tcgaaccgct gaccctctgc
      541 ttgtaaggca gatgctctcc cagctgagct aaacctccaa cattcaatta aaatgacccg
      601 tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg cttgaccaac
      661 gggccaggta aatttgctac ggagagtaag ggattcgaac ccttgagaca gtttggcacc
      721 atctacatga tttccaatca tgctccttcg gcctctcgga caactctcca gagtgaaaga
      781 agctcaatac tctttctcaa aatgagaact ccggcagtag gactcgaacc tacgacatca
      841 tgattaacag tcatgcgcta ctaccaactg agctatgccg gaataacat
//
LOCUS       DADVXO010000285          879 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.293, whole genome shotgun sequence.
ACCESSION   DADVXO010000285 DADVXO010000000
VERSION     DADVXO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.293"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>879
                     /locus_tag="KB950_002908"
     CDS             <1..>879
                     /locus_tag="KB950_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HBB6859045.1"
                     /translation="CALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVS
                     YDKEPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMA
                     MTNGVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGV
                     TDANYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLIL
                     VKPLGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGAT
                     SQLKMMSITYLDWIVLA"
BASE COUNT          286 a          129 c          154 g          310 t
ORIGIN      
        1 tgtgctttat taattagtct gaacacagag atccttatgg taaaacttat tactgctata
       61 atttatcttg cttgtccaat atattataga tggtatgtgg atcgaaacta taatataaag
      121 aggaatgtgt catatgataa ggaaccgatc cctcaaaaat ggaatggggt tgcacaacac
      181 gtagcttctg ttgtactaga tagtactgat gttgtagtgt tatctatatt ttctaacctg
      241 atatatgtat ctatatataa tgtgtacaat ttagtaatat acggagtaaa acagctattc
      301 atggctatga caaatggcgt tcaggcctta ctaggggaac tttgggtcag aaaagaaata
      361 gaagttcttt acaaaacatt tgcatggttc gaatggattg tacatacttt gacagttttt
      421 atttttggct gtaccggtat actaatcgtt ccatttattc aagtatatac aagaggagta
      481 acagatgcta attataatca attatcattc gcagctttac ttgtaactgc taacgcactt
      541 cactgtctgc gtacacctta tcatgtaatg ataaaagcag ctggtaaata taaagaaact
      601 caaaatagtt ttataattgc ttctttgtta aatttaggtt tatcattaat tttggttaaa
      661 ccactagggc tagttggagt agcattagga tctctaattt caatcacata tcatacactt
      721 tggagtgcaa attataattc aaaatatttg cttaaatggc cgatgaaaaa attctataaa
      781 cagttatttg taaacatctt aactgtagtt ctttcttttg gggcgacatc acaattgaaa
      841 atgatgtcaa tcacatattt ggattggatt gttctcgca
//
LOCUS       DADVXO010000286          857 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.294, whole genome shotgun sequence.
ACCESSION   DADVXO010000286 DADVXO010000000
VERSION     DADVXO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.294"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..116
                     /locus_tag="KB950_002909"
     CDS             <1..116
                     /locus_tag="KB950_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBB6859046.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
     gene            244..795
                     /gene="raiA"
                     /locus_tag="KB950_002910"
     CDS             244..795
                     /gene="raiA"
                     /locus_tag="KB950_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBB6859047.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          306 a          151 c          177 g          223 t
ORIGIN      
        1 aaaaaatatt gattattgac gatgtctata caacaggaca gacgcttttt catgcagcag
       61 attgcttgca gaacgcacag ccgaaaatca tccgaaccat ttctttggca agatgaatca
      121 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
      181 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
      241 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
      301 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
      361 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
      421 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
      481 ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
      541 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
      601 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
      661 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
      721 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
      781 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
      841 agctgccgca agttttt
//
LOCUS       DADVXO010000287          851 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.295, whole genome shotgun sequence.
ACCESSION   DADVXO010000287 DADVXO010000000
VERSION     DADVXO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.295"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            104..796
                     /locus_tag="KB950_002911"
     CDS             104..796
                     /locus_tag="KB950_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6859048.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          375 a          132 c          147 g          197 t
ORIGIN      
        1 gtatttatat taaaattaca tatgtaattt aaaaacgctt gacattgtgt ttgagatcac
       61 ttacttttag catgtaaaag tattggatta aggagctatg caaatgaaaa agaaatttat
      121 tactggtggg attgtcgttg catttctatg tctaattggc tacacaggca cgcggtacgc
      181 aaatgaaatt attttgtggg gtggagagac agatattgat acaatcaatg agaatctgac
      241 acagcttgat catacattga cagataaaga gcaagcgatt acagatctta ccgctcgact
      301 agcacaaaaa gaacagacat tgcaagattc aaaggcagat gtccaagaat accaaacaaa
      361 agtaaatgaa ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa acttagaaag
      421 tcaattagtc agcaaggatg cacaactaca aagcaaacaa aatgaaatca atgtcaaact
      481 agatgagatc aaccaaaaaa atgatgaaat caatcatctg aatgcagatt ggacagcaag
      541 actacaagaa gcacaagcaa aactttcaga attacaaaat actgtaaatg gtttgaatca
      601 acaaatcgca acactgacac aagaaaaaac aaatgtagaa actcagttaa cagatacaca
      661 aaagaaatta gacgaagcaa ctgctgaaaa ctcaagctgg aaacaatata ttcaaaagtt
      721 agaaaaagca aaagatgaag tggaagatac agctgataaa tctcagcaaa ttgttgaaga
      781 acatttgcgt aaataatctt tcttaacaag taaacctaaa ttagtaaaga agcaacagta
      841 atctatacta a
//
LOCUS       DADVXO010000288          847 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.296, whole genome shotgun sequence.
ACCESSION   DADVXO010000288 DADVXO010000000
VERSION     DADVXO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.296"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..823)
                     /locus_tag="KB950_002912"
     CDS             complement(<1..823)
                     /locus_tag="KB950_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859049.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADIL"
BASE COUNT          283 a          101 c          147 g          316 t
ORIGIN      
        1 ctaatatatc tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt
       61 tttccatttt tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat
      121 gtttttttaa gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat
      181 tttctgaaag atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa
      241 gaatatgctt ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt
      301 cctgttgata atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta
      361 ataaggaaag caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct
      421 gatcatagga aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt
      481 tcagaaaagt aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat
      541 gtgctaataa ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt
      601 gagtagaaaa gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta
      661 gttctataga tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc
      721 gaaaataggg agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt
      781 tgacaagatt ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta
      841 tataaaa
//
LOCUS       DADVXO010000289          843 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.297, whole genome shotgun sequence.
ACCESSION   DADVXO010000289 DADVXO010000000
VERSION     DADVXO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.297"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>843
                     /locus_tag="KB950_002913"
     CDS             <1..>843
                     /locus_tag="KB950_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HBB6859050.1"
                     /translation="NSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILG"
BASE COUNT          306 a          106 c          150 g          281 t
ORIGIN      
        1 gaattcttgg atttatcaaa tccaattaac atcggacgat aaattatatt ccttcatcaa
       61 tgatattaat agttggtgta gtcgagaagt tcaattagtc aatcgtttta ttcaaatagg
      121 ggatatttat ttaacaaaaa ttcaaaaaaa tgttttggtt attttattta agttaatgtg
      181 tagtaaagaa tttcagcaaa aacgtttagc actgattttg gctcatggag atctaacggc
      241 aacctgtata gcaagtactg ctaataggat cgttgagaga tatatatacg acgccattga
      301 tatgccacta atgacttcaa gtagtgagat tgcagacaaa attaatagat acttacaaga
      361 gtgtaccgga tatgaagaaa ttattttact tgttgatatg ggttcactgg agaaaattta
      421 tctaaatttg gaactagacg ctcaaaaaaa attcggacta ctaaacaatg tttcaacaag
      481 aatggctatt gatgttgcaa atcagatatt attagattta ccgcttaaac agattcttga
      541 acaagtaaaa gaaaatcaaa atgtggatta tatttatgaa tcatcaaata ttaagaagga
      601 tatgattgtc tgttcttgtg caagcggatt aggaacttcg tttaaattaa aaaggatact
      661 tgaagatagc tttccaaggg atactgatct tgtcatagaa acgtgtgact attactcttt
      721 agtgaatgaa aactatttat cggagcttcg taaagagaac aatattcttt ttattgttgg
      781 aacactaaat ccacatctag aagatgtaga attcttttca attgaagata tgattttagg
      841 aat
//
LOCUS       DADVXO010000290          826 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.298, whole genome shotgun sequence.
ACCESSION   DADVXO010000290 DADVXO010000000
VERSION     DADVXO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.298"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            14..313
                     /locus_tag="KB950_002914"
     CDS             14..313
                     /locus_tag="KB950_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6859051.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            578..>826
                     /locus_tag="KB950_002915"
     CDS             578..>826
                     /locus_tag="KB950_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859052.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAV"
BASE COUNT          346 a           82 c          174 g          224 t
ORIGIN      
        1 aggagaggtt tttgtggaag caataaaatt aatgatttca gaagaacagg aggaaatgtt
       61 tctaaagcag ttttatcaaa ttgctacaaa agctattgaa aaggctcaag aaaatgcggg
      121 gatgacaagg gaatatctca atcaaaaaga aatggcggaa tacttaaatg tgagtgaaaa
      181 ctttttaaaa aatgctgtga gaaatgaggg attgcccgta gttgaattag gggcaagaaa
      241 attttattcg aaaaaggcag ttaatgagtt tatgtttgct cgaatgaaaa gaggggaaaa
      301 tattggaaga tgatgaagta gaaattataa aaaaatgctt taaaaaaaga aaaaaagtgt
      361 tcccaaaagt ttgcagactg ggggaacact caataataaa gtgctgagaa aactttattt
      421 atagctatta tatcaaaaaa gaaggtgaaa aggtgttttc ttctggaaat gttgtagttg
      481 acaaacttag agaaataaat tttgacggaa atgtaatcca cacacatggt acgatcaatt
      541 aagaaaaaaa acgaaaaaag gtgttgagaa gccctatttg aatgcaatag ttatcttagc
      601 agaaattacc tattggtata gacctaaaaa aatttttaac gatgatggtc agctacttgg
      661 ttatcaaaaa aagtttcttg aagatatttt gcaaaaatca tataagcaat taagcaaaaa
      721 aacaggatta tcagaacgag ctataaaaga tgcaattgtg tacttagaga aaaaggggat
      781 tatcaaaagg gtttttagaa atatttatta tgagaaaaga gcagtt
//
LOCUS       DADVXO010000291          823 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.299, whole genome shotgun sequence.
ACCESSION   DADVXO010000291 DADVXO010000000
VERSION     DADVXO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.299"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(57..302)
                     /locus_tag="KB950_002916"
     CDS             complement(57..302)
                     /locus_tag="KB950_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6859053.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(296..697)
                     /locus_tag="KB950_002917"
     CDS             complement(296..697)
                     /locus_tag="KB950_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6859054.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          266 a          143 c          111 g          303 t
ORIGIN      
        1 ccttaactat tagaagattc ttttaaacag aaaaagtgga aaggggattt ttttaattag
       61 ttaattttaa attttaagat cgcttctatt aatttaattt ctagctgact tatcatatat
      121 tcatctacat aggtaacgaa ttgacctacc tcgttaaatc catttgtcaa acataactta
      181 gccatataag aatgataatg tttgagtatc atcttgatcg aaattggatc tccttctaga
      241 gcagataaga taatggatag tggaactaat aggtactgcc gctttttaga tttatctacc
      301 ataggtcata ccccattttc aattgttcaa ctgccttctt tcgttgatac cagataccac
      361 ttgtgctcat attgagaatt ttaccgattt cagcatcatt aaaacctgca aaataataaa
      421 gtaggataat ctttcttctt atctccgata actgatcaat tgctgctgat aacttttgat
      481 ctgaaattaa tatttccatt ccaaataaga aatagacatc ttgttcatca aaatgagttt
      541 gggaatacct gccttcattt gaatctgcca atggttcctc attatccttt atgttagcgt
      601 ttcgttttcg aatagactta gcttcgtttc gtaatacttt tttacagtag gcatcaaatt
      661 ggtgttgtat agatatgtca ttgtaatgtt gttgcatgat aatatgctcc tttttccccc
      721 tttccactaa tacaacaatc agaaagcccc tttttttcga aaaaactaga aaaaaaacat
      781 aaaaatattt atcattcttt aaacagaaaa tctttctata aaa
//
LOCUS       DADVXO010000292          815 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.300, whole genome shotgun sequence.
ACCESSION   DADVXO010000292 DADVXO010000000
VERSION     DADVXO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.300"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            78..311
                     /locus_tag="KB950_002918"
     CDS             78..311
                     /locus_tag="KB950_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBB6859055.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            308..715
                     /locus_tag="KB950_002919"
     CDS             308..715
                     /locus_tag="KB950_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBB6859056.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          309 a          116 c          148 g          242 t
ORIGIN      
        1 catgagaggg actatgcttg atttaaaatc aacaagcgta tatactgtat atacaaagaa
       61 aaaggaggga gaagactatg gcaattaaag aaagaaaatt tagggaagtg gggaactcag
      121 tagtattaac tatttcaaag gagtttctta aagaaaatgg gttaaatccc ggagatacgg
      181 tttttttaga tgaagaaaaa cttcgagcgg caattacaaa aaaacaagcc ttatctgatg
      241 aaattgattt agcaatatct caagcattaa ctgaatatga tactactttt agagagcttg
      301 tcgataaatg agtattcatt acctagaagc caaagatatt ataaaaatga atgtaatcca
      361 aataaaaaaa tactctccta atgaacccct tggtgtaaag gatgcgaatg ctttagaaat
      421 gtgtgtaggg caacttcgag cctcagcttt tggtgaagag gtttatcctt cgatttatga
      481 aaaagcagct atcctactta tacaattgat aaagaaacat ccttttcaca atgcaaataa
      541 acgtactgct ttcttagcta cttttgtctt tttgaaaatc aatggacagc ttttagctat
      601 tgatcaaaaa gatgctgtaa atttagcggt atatattgct acgtatgata aggatttcga
      661 tcaactaaaa tatgaagtga tcaacactat aaagacacat acgcagccca tttaaggagt
      721 tactgtatgt gtctttatag ggttattagc tgctatagcg aaataattga agtaaattac
      781 aaatagctat aattcaaatc caactatata gaaaa
//
LOCUS       DADVXO010000293          808 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.301, whole genome shotgun sequence.
ACCESSION   DADVXO010000293 DADVXO010000000
VERSION     DADVXO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.301"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>808
                     /locus_tag="KB950_002920"
     CDS             <1..>808
                     /locus_tag="KB950_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6859057.1"
                     /translation="LISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNV
                     KGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTG
                     VNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDA
                     DNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQ
                     DATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSI"
BASE COUNT          345 a           94 c          146 g          223 t
ORIGIN      
        1 tctcattagt ttatctatta aagatgctac ggacagagct tcaaaagaaa catttgctaa
       61 tataacaaat agtttttcta tggagataaa tagacaagta aatccaggaa cacctagagg
      121 tgggggaaat gtaaaaggtg aagatattaa aaagatatct caaacagata gtatagactc
      181 ctatgtaaaa agaataaaca gtgttgcaga tttagttgat tatgatatta ttgaaactca
      241 agagactctt gcgaatcaat cacctgaaag agcaaagaat tttaaaagaa cagttatgtt
      301 aactggggtt aacgactcaa caaaagaaac aaaatttgta tcagaagcat ataaattagt
      361 agaaggaaag catttagaaa atgaagataa aaataaaatc ttaatgcata aagatttagc
      421 tgaaaaaaat aaccttaaag ttggagataa gataaaaata aaatctaatt tatttgatgc
      481 tgataatgag aaaggtgcag atgaaacagt agaagtagaa attaaaggtc tattcgatgg
      541 gcataatagc ggtggagtaa gtgcagcaca agaactatac gaaaatacat taatcactga
      601 tattcataca gctgctaaag tttatggtaa taccgaagat acagctgtat accaagatgc
      661 aacattcttt gtaaaaggtg ataagaatct tgatagtgta ataaaagatc ttgggaaatt
      721 agatataaat tggagagaat ataatttgat taaaagttca tcaaattacc ctgcattaca
      781 acaatctata tcaggtattt actcaatt
//
LOCUS       DADVXO010000294          803 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.302, whole genome shotgun sequence.
ACCESSION   DADVXO010000294 DADVXO010000000
VERSION     DADVXO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.302"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..394)
                     /locus_tag="KB950_002921"
     CDS             complement(<1..394)
                     /locus_tag="KB950_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBB6859058.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKK"
     gene            complement(399..584)
                     /locus_tag="KB950_002922"
     CDS             complement(399..584)
                     /locus_tag="KB950_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6859059.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          247 a          161 c           96 g          299 t
ORIGIN      
        1 ccttttttac aacatggatt ttcgataaaa actgttgaaa ttcctgtata tgaattggtc
       61 gaatctttac aacctgcatt tctccaaaaa aatccaatgc acgttgtaaa atatgatctg
      121 ttttcatata tgttttttct acagttccca acttatacca aggaaaaaaa tactctcgat
      181 aaaaatcatg aaacgttatt ccttgcagat aaattccttg tgaatcagaa aaactttgtt
      241 ttaactcttc gagtcccctt ctagcttctc cttgagtttt gaagcctctt ctggtcactt
      301 gaattctttt tccaaagcga tcttgtccta aagttgcaga aaaaaaccaa gtgcctcttt
      361 ttctgtcttt ataaacattc gcaattttcc tcattttact attcctccca agaaatatgg
      421 agaacttctt ctaaaattga aataggaatt ctttttattt ttgaattacg ataaaaaaag
      481 tatcctttct caactaaaag ttctttacat tccttgaaga ttttattagc atagtactga
      541 gaaaaaccta gattaatcaa atcttttcga gtagctgttt tcattatagc accttcctta
      601 ctttttttca tttatttcta tcagctgctt ttgtcaaaag cacataggaa tttcacctct
      661 ccccaagttt acggcgagcc ttacggaagt atcattatcc caactttaaa tcatcgcttt
      721 ttattgacta taaaaattat tttagaatat ttatcgctcc ttctttacag caagaaatca
      781 tggcgcaact aaaatatgct cct
//
LOCUS       DADVXO010000295          795 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.303, whole genome shotgun sequence.
ACCESSION   DADVXO010000295 DADVXO010000000
VERSION     DADVXO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.303"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            16..423
                     /locus_tag="KB950_002923"
     CDS             16..423
                     /locus_tag="KB950_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859060.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            437..>795
                     /locus_tag="KB950_002924"
     CDS             437..>795
                     /locus_tag="KB950_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859061.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSD"
BASE COUNT          292 a          107 c          142 g          254 t
ORIGIN      
        1 aattaggaga aatcaatgtc ttatagagta caattaataa tttcagaaga tgtagaaagt
       61 cagcaatttg gcactaatgt tattaataaa gtaattaatc cagctctttc tattaatgcg
      121 cctttaattc ctactgcttt atcattttct gtaacagctg tggtgtctga gatagaggat
      181 acagaaaaga taaaaatagt agaaattgaa gttttaaaca agaatgaaaa acaaattttt
      241 tctacgggtg aagtatcggt aagcttgcct cctcaagtta acgatataaa ctttaatata
      301 aacgccagaa atgtcttggt tgaagaagca ggagagcatt atgctgttgt taaatttaat
      361 ggaactgaga ttggaaggca aatctttgat atcaaggtca acaaaccagt ggaaaaaaat
      421 taaggagacg atacggatgc ttgatatagt accatcaaat acacatcgga gtggtagctt
      481 aataaatttt gctgcgattt ttgcttgcgc atcggtaatt ctagcatctc cagttgttgt
      541 ggatgcaagt tctactccga aaactaaaaa tgataatcaa cttgtaatta ctacaaaaaa
      601 tgagattaac acaatttcaa gtaatgatgg caaaattttt gatgtgataa atacggtttt
      661 aaaaagaaaa gctgaaatca atataaaata tgatgaagat ttaaacttgt atttctttgt
      721 tatcaaaaca acatctgaat tgttttcctc agattacgat gttttggata ctctcgataa
      781 tgtattatct gacta
//
LOCUS       DADVXO010000296          792 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.304, whole genome shotgun sequence.
ACCESSION   DADVXO010000296 DADVXO010000000
VERSION     DADVXO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.304"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            3..>792
                     /locus_tag="KB950_002925"
     CDS             3..>792
                     /locus_tag="KB950_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HBB6859062.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRV"
BASE COUNT          272 a          115 c          144 g          261 t
ORIGIN      
        1 atatgaacga ttttactaca gaaattatgg aaatactaat caataaaggt gatttggatg
       61 atttgtttcg tcgtcaccta gaattggcta ttaacgcttt attacaagct gaattaacgg
      121 tatttcttga ctatgaaaag tatgatagag ctggatttaa ttcaggtaat tcccgtaatg
      181 ggaattattc gcgctctttt aaaacagagt acggtgaatt gaatttagta atacctagag
      241 ataggaatgg agacttttct caacaaactt tacctgctta taaaagaacc aatgactctt
      301 tagaaaccac tattattcag ctagttcaaa aaggaattac tatgtccgaa atctctgatc
      361 taatcgaaaa aatgtatggt cattattaca aaccacaaac tatttcaaat atgagtaaaa
      421 ttgtgtctga agatgtttcg gcatttaaag aaagaacttt agaagctaaa tactcagtca
      481 tttttatgga tgctactcat attcccttaa aaagacaaac cgtatcaaaa gaagctgtct
      541 atatagtaat tggtatcagg ttggatggaa ctaaagaggt tctaggattt actatagctc
      601 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga tctaaaagac cgtggtttag
      661 aagaggtttt gttagtcgta acagatggtt taagtggtat tgacgatagt attcatagtg
      721 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
      781 aggttcgtgt ta
//
LOCUS       DADVXO010000297          777 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.306, whole genome shotgun sequence.
ACCESSION   DADVXO010000297 DADVXO010000000
VERSION     DADVXO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.306"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            93..296
                     /locus_tag="KB950_002926"
     CDS             93..296
                     /locus_tag="KB950_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859063.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            293..565
                     /locus_tag="KB950_002927"
     CDS             293..565
                     /locus_tag="KB950_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBB6859064.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            566..751
                     /locus_tag="KB950_002928"
     CDS             566..751
                     /locus_tag="KB950_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859065.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
BASE COUNT          298 a          109 c          166 g          204 t
ORIGIN      
        1 tgattaatcg atacaagtat tatatcaaat aaaaaagctg atttaggggg tcttacaaaa
       61 acactaaaat tttttcaaaa agaggaggaa cggtggtgaa agtcagagaa caattcaaga
      121 tagtgagcga cgaaggtaat gtttacaaaa taaagatcgt taatattaat gaatatcgag
      181 aagcagatca caaatatttg gttgacttat acatgaacga taaatatttg ggagaaggat
      241 tttgtggaga ttcacttttt gatgatgagc gaattttgaa ggaggaaaca gaatgatcca
      301 cgaactaaaa atactaccag aatattttga agcagtgacg agcggacgca aacaattcga
      361 gatccgcaag aatgatcgtg acttcaaagt tggtgatcag ttaatactac gtgaatataa
      421 aaaatacatt aaacatgaat tacctggact aacgagacca acatttagtt acactggtga
      481 ctcatacaaa gcagagatca cttatattac tgattacatg cagaagtccg gatatgtagt
      541 gttggggatt aaaggggtga attaaatgaa agtatacatc ttagctatca cagagggcac
      601 atggatgttc ccagtcggtt ccggaaaaat ctataaatca aaaacagctg cttataaagc
      661 atttgaaaaa tacaaaaaag aaaacggtgg cggcactaac gcaaagattc tagtggctga
      721 taactggcat gaagaaggtg aacgtaattg atttacctgt tcattttcgt ggtacta
//
LOCUS       DADVXO010000298          720 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.308, whole genome shotgun sequence.
ACCESSION   DADVXO010000298 DADVXO010000000
VERSION     DADVXO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.308"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..483)
                     /locus_tag="KB950_002929"
     CDS             complement(<1..483)
                     /locus_tag="KB950_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB6859066.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     S"
BASE COUNT          254 a          114 c          102 g          250 t
ORIGIN      
        1 gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac agtctacaaa
       61 atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa
      121 atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa
      181 tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa
      241 tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa
      301 atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat
      361 tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt
      421 ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat
      481 catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat
      541 tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga
      601 aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag
      661 aataaaaatt tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac
//
LOCUS       DADVXO010000299          701 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.310, whole genome shotgun sequence.
ACCESSION   DADVXO010000299 DADVXO010000000
VERSION     DADVXO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.310"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            147..629
                     /locus_tag="KB950_002930"
     CDS             147..629
                     /locus_tag="KB950_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859067.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          202 a          105 c          123 g          271 t
ORIGIN      
        1 aataatcaat tactttttca ttatttctag attaaatgaa taaactctca tgccattatc
       61 tctaaaaaaa aagtttttcc ccctaatttt tttagataga aaattgtata gtatgatgag
      121 agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat cggctttatt
      181 tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat gctgttgtac
      241 aaggtgcgga gatattaggc ctgatccaag gaagcggatt tgtggtttct aggaattttc
      301 cagctttgat tgttttctat ttagtgataa gtgtaggtct cttattacca aaatctctga
      361 tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt acatctacgt
      421 tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt ggattgaagg
      481 atttagctta ttcctccatg tatttcttga cgatcaaacc atggttctat ttagtaattg
      541 cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata aaaagtgaac
      601 gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa acatatgagc
      661 tgcgtaaaat gcatgtttct catatgcttt ttttattatt a
//
LOCUS       DADVXO010000300          700 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.311, whole genome shotgun sequence.
ACCESSION   DADVXO010000300 DADVXO010000000
VERSION     DADVXO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.311"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            23..694
                     /locus_tag="KB950_002931"
     CDS             23..694
                     /locus_tag="KB950_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6859068.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          280 a          108 c          112 g          200 t
ORIGIN      
        1 taaaagttaa ggagcattgt gtatgacaat taaaactagt ctccaaaatc aagcttacca
       61 atctattcgc cgacaaatta tctatgctga cttagaacct ggaaaaaaag tatcagaaaa
      121 aaaattagaa gaaacacttt ctattggtcg tacaccaata cgtgaagcac tgattcaact
      181 ccgacaacaa ggtcttgttg atacaattcc tcaaagcgga aattatatct cattaattga
      241 tttagattcg gccaaaaatg ctcgatttgt cagagaacat ttggaacgac aaattatgtt
      301 agagtgttgt gccaaaatga ataaccaaaa aaaacggatt ttagaaacta taatttcaga
      361 acaagaaaaa gctgttagtc aaaaaaatgc tcgtagtttt tttcataatg ataatttatt
      421 ccataaagct tgttttgaaa ttgcagagcg agaagaagta tggcaatggt tagaaaataa
      481 caatacacac ttagaacgat ttcgatggct acgagtaaaa acggaaggat taaagtggga
      541 aaaaattatg gaacaacatt atcaactatt tgaggcatta acaaaacaag atccagaaga
      601 agctaacttt ctaacatcgt tgcatcttca tttaatgctt aatgagcaaa atattgtttt
      661 agctactcat ccaaaatatt tcaaaaatat ataactttta
//
LOCUS       DADVXO010000301          691 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.312, whole genome shotgun sequence.
ACCESSION   DADVXO010000301 DADVXO010000000
VERSION     DADVXO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.312"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>691)
                     /locus_tag="KB950_002932"
     CDS             complement(<1..>691)
                     /locus_tag="KB950_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6859069.1"
                     /translation="MLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKT"
BASE COUNT          224 a          122 c           89 g          256 t
ORIGIN      
        1 tgtttttaaa ttcaaacctg ctgcgtaaaa attgtaatta aatatagaaa agccatttgt
       61 aaattgggaa ccagaaggtg taaaatcttg attgttaata tgttgagttg caatataagc
      121 tgttcttaga gcattagaaa tagaaaaatc aacactactt ctattaatgg acgtaaaaaa
      181 agttgtagca caatccccaa tagcatatat atcttttata tttgtttgct ggtattcatt
      241 agttagatat gctccactaa catctctcaa gagttccttt tccccaattg gactattaga
      301 tagaaatcct atagcttgta cgaccatatc catagagtat tgggtaccac ttgtagtgac
      361 tattttttca atcttttttc ttcctaaata ttttttagga ctttctgata gacaaagatg
      421 tattccattt tttgatagta tttcttcaac tttatctgaa aaactacggt cgtaataagt
      481 cgacaacact ctatctgcaa catcgaaaag atatacttct ttccctcttt ttcttatggc
      541 ttcagcaatt tcaactccaa tatatcctgc tccaataatt cctacttttt taatagtact
      601 atcatccaaa gctttaaaaa tctcttttgc atttggaaaa ttctttatcg aaaaaatatt
      661 agctaaatta ttccccggta tatctagcat a
//
LOCUS       DADVXO010000302          676 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.313, whole genome shotgun sequence.
ACCESSION   DADVXO010000302 DADVXO010000000
VERSION     DADVXO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.313"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..448)
                     /locus_tag="KB950_002933"
     CDS             complement(<1..448)
                     /locus_tag="KB950_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBB6859070.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          200 a          136 c          103 g          237 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tctatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtatt
//
LOCUS       DADVXO010000303          660 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.315, whole genome shotgun sequence.
ACCESSION   DADVXO010000303 DADVXO010000000
VERSION     DADVXO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.315"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..446)
                     /locus_tag="KB950_002934"
     CDS             complement(<1..446)
                     /locus_tag="KB950_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6859071.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          195 a          118 c           90 g          257 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
//
LOCUS       DADVXO010000304          658 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.316, whole genome shotgun sequence.
ACCESSION   DADVXO010000304 DADVXO010000000
VERSION     DADVXO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.316"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            224..>658
                     /locus_tag="KB950_002935"
     CDS             224..>658
                     /locus_tag="KB950_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HBB6859072.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKD"
BASE COUNT          241 a           74 c          128 g          215 t
ORIGIN      
        1 atttataatg tttattgatg taattgaaga aacatcaaga aattttttgc tgtctaagtt
       61 cagaaaattt cgtatgaaaa atgataaaca aaaattattg gcaaatgaga tttggagtag
      121 tttagaagtc gaagaaagta aacaagaact tttgttgaaa tggatacttt ttcttgtaaa
      181 gaagttctat gtgaaaatac cagaaaccca ttgtctgata gttatgcacg gtaaaataac
      241 agcttctgcg atcgcaaggg aaacaaataa gttgctgaat acatatgttt atgaggcttt
      301 cgatatgcca attgaaggtg agactagtga cttaattaac ctaatcaaca atttttgtaa
      361 aagtatagat acgacgaatg gactcattct gctagttgat atgggatcat tggaacaaat
      421 gtatgaaaaa attgaatcca atgtaatagg tgatttggta attttaaaca atgtttctac
      481 agctttagcg attgaatgtg ggattcaagt ttgccaaaaa aagccaatta gtcattttta
      541 ccagatggat tttgattcgt ttcaagttaa agttcagtat tataaaggat tatcacaaaa
      601 gaaaaatgta atagtatctt gtctctctgg tgaggggatt tctgaaaaag ttaaggat
//
LOCUS       DADVXO010000305          643 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.318, whole genome shotgun sequence.
ACCESSION   DADVXO010000305 DADVXO010000000
VERSION     DADVXO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.318"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            16..643
                     /locus_tag="KB950_002936"
                     /pseudo
     CDS             16..643
                     /locus_tag="KB950_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          228 a          113 c          109 g          193 t
ORIGIN      
        1 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
       61 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
      121 attaaaaacc gaagaaatac tgatcaactg aagtaacgcg atacagtgat tattgcttgt
      181 gttatatggg gcataattaa tggctatact agccaaagag ccacgtatag agcggtttgt
      241 tccgtcttat ttcctaatgg tgactttcct agtaggagtc gattcactcg cttaagttca
      301 aacttagctt acactatcaa gattattcga tactttttca tcaagaagct caccaaaggt
      361 gaactagtcg gaattataga tagttttcct agtccattat gtaaaccagt tagaaataga
      421 caagcaaaac tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat
      481 ttttatggtc ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca
      541 atcacgaatc caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat
      601 cttcccaata tcctagggga caaaggatat attagccata aaa
//
LOCUS       DADVXO010000306          628 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.319, whole genome shotgun sequence.
ACCESSION   DADVXO010000306 DADVXO010000000
VERSION     DADVXO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.319"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
BASE COUNT          215 a          114 c          101 g          198 t
ORIGIN      
        1 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
       61 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
      121 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
      181 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
      241 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
      301 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
      361 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
      421 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
      481 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
      541 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
      601 aaaaccacca ttacctgtag ataaaaac
//
LOCUS       DADVXO010000307          625 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.321, whole genome shotgun sequence.
ACCESSION   DADVXO010000307 DADVXO010000000
VERSION     DADVXO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.321"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            1..625
                     /locus_tag="KB950_002937"
                     /pseudo
     CDS             1..625
                     /locus_tag="KB950_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DADVXO010000308          609 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.322, whole genome shotgun sequence.
ACCESSION   DADVXO010000308 DADVXO010000000
VERSION     DADVXO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.322"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            6..>609
                     /locus_tag="KB950_002938"
     CDS             6..>609
                     /locus_tag="KB950_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859073.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLD"
BASE COUNT          249 a           98 c           95 g          167 t
ORIGIN      
        1 aaaagatgaa gccaaatcct tcagaagcag gaaaacgaat caaacaatta cgcctttcat
       61 gtggttttac tatggaagaa cttggaagga aaatagataa ttcaccacgt gcgaccatat
      121 ccaactggga acgtgggacc aatttaccaa atccacaaaa gctgaaatta ttatcaacta
      181 tcactaattc aacaattgac tggattaaat ggggaacttt agaagaatat ataacttcat
      241 accttatcga tatagggtat gagctatata ttaaggactt cccagaaata cctcacaaag
      301 tctttaaaga cattcaagaa agatatagtg atacgttttc gctagataaa gactatgagt
      361 tattgaatcc tataataaaa aatatattta caaaatatta ctcaaaagat tttgaaattt
      421 atctaaatga gatacttgct aataatatca atcctctgat tgaagagtac gctaaccaaa
      481 gacactctat ctctaaagag ctatttttca aaagagccaa cagtaaactt caagacaaga
      541 taagaaatgg tgatttcaaa tacggtgaaa aagaaagaat gattgattct ttaaaacagc
      601 tattagacg
//
LOCUS       DADVXO010000309          609 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.323, whole genome shotgun sequence.
ACCESSION   DADVXO010000309 DADVXO010000000
VERSION     DADVXO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.323"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            227..>609
                     /locus_tag="KB950_002939"
     CDS             227..>609
                     /locus_tag="KB950_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HBB6859074.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIA"
BASE COUNT          203 a           86 c          106 g          214 t
ORIGIN      
        1 tatagacaac caagatattg aaaatcatcc tatgaagatg tttttggcta taactacagc
       61 aaatattata atgggttttc ctagagctgc ttcatattta attgtgaaaa attttgtgta
      121 tggtgtttta attatattgt tgataaactt tttcctacag caatttaata aacgtcttta
      181 atatttaagc ttaataaaaa gataaaacaa gtcaggagtt gcaaaaatgg aaagaactgc
      241 tcggctcaaa aaaaacacta tttgggctct ggtgtttcaa ataacttcta ttgtctgtgg
      301 tatgatcttg ctacgtttga tattaacaaa gtacggatcc gaggttaatg gaatgattag
      361 ctctattaac caatttctac aaattatttc ttttttacaa ttaggagtag gcgctgtaat
      421 gcaatcaact ctatacaaac cactagcaga aaaggataat ttaatgataa gtcaaatctt
      481 agcttctgga gggaagtttt atcggcaaat tgcatatatt ctactagtat acgtgggttt
      541 gttacttgca gtctattatg ttctcgttcc taatgaattt gggagatcat atacaatggc
      601 aatgattgc
//
LOCUS       DADVXO010000310          564 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.324, whole genome shotgun sequence.
ACCESSION   DADVXO010000310 DADVXO010000000
VERSION     DADVXO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.324"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(123..489)
                     /gene="ssrA"
                     /locus_tag="KB950_002940"
     tmRNA           complement(123..489)
                     /gene="ssrA"
                     /locus_tag="KB950_002940"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          177 a          115 c          104 g          168 t
ORIGIN      
        1 ctttttgaat gctattttga atgccatttt cattcattta acgaaaatct gcgaaaataa
       61 aaaatgctca aacccttata aaataaggat ttaagcatct agcgaaatct gctgaaatct
      121 cacaatggag acggcgggag tcgaacccgc gtccaaacac atcgccactt aaaaatctac
      181 gttcatagtc aactcattta aggattcact ttacagcttg ccgagtgaca ggcattctgt
      241 atcgctagtc tgatgttctc tttttcaact tacagacgga aagttgaaac gtatcccact
      301 aaagttagga cccttactcg agcacatggg cgatgccgag aggatctacg ctaactgttt
      361 ttaggcagct aaagcgtaag agttgttttc gtttttagca gttatattta actgtaacgt
      421 ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt
      481 aacgtccccg ttagctaaca agggttacgt gtatttttca tacaaaatta gtatagcata
      541 tattgggata aattcaataa aaaa
//
LOCUS       DADVXO010000311          552 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.326, whole genome shotgun sequence.
ACCESSION   DADVXO010000311 DADVXO010000000
VERSION     DADVXO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.326"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..450)
                     /locus_tag="KB950_002941"
     CDS             complement(<1..450)
                     /locus_tag="KB950_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6859075.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          156 a          101 c           68 g          227 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca ac
//
LOCUS       DADVXO010000312          546 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.327, whole genome shotgun sequence.
ACCESSION   DADVXO010000312 DADVXO010000000
VERSION     DADVXO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.327"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>546)
                     /gene="wzy"
                     /locus_tag="KB950_002942"
     CDS             complement(<1..>546)
                     /gene="wzy"
                     /locus_tag="KB950_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HBB6859076.1"
                     /translation="ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFN
                     YKKLIKWLILAVIGIYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGG
                     TMSIITFFLISGNGIYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITW
                     GTGFSMIAEAYVNGGYIGGIVY"
BASE COUNT          209 a          105 c           66 g          166 t
ORIGIN      
        1 atagactata cccccaatgt atccaccatt gacataggct tctgcaatca ttgagaatcc
       61 agtcccccaa gtgattccta aataatcctg agaaaaccaa tcagctaata aaacaaactt
      121 tattccaaac attgatagca ttcttgttga tatagagcct aagagtgagg tcaaataagt
      181 attagcaaag ggatatattc cattaccact tattaggaaa aaagttataa tactcatagt
      241 cccccccatt tcaaaaaaag aatcaactaa aggagaatca tctgaatcaa atacagatgt
      301 aaaatctttt aaagtaagtt tacttagtcc tccaccatca cgtgctttcg ttattacgtt
      361 tagaatatat attcctataa cagctaaaat aagccacttt attaattttt tataattaaa
      421 ggttccttta tattgagtgt atgcccatat gagtataata gagctataca accaacttcc
      481 tctgtctcct gacataattc ctaataaaga atataaagca aaaataataa aaacaaatct
      541 cgttat
//
LOCUS       DADVXO010000313          539 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.328, whole genome shotgun sequence.
ACCESSION   DADVXO010000313 DADVXO010000000
VERSION     DADVXO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.328"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..72)
                     /locus_tag="KB950_002943"
     CDS             complement(<1..72)
                     /locus_tag="KB950_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB6859077.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGK"
     gene            complement(202..>539)
                     /locus_tag="KB950_002944"
     CDS             complement(202..>539)
                     /locus_tag="KB950_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6859078.1"
                     /translation="ELDPFINYIFENKRIFDLILFQSAGTKYQDIVEKITSFVTKKTV
                     CCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFS
                     VPGWIELLS"
BASE COUNT          198 a           85 c           72 g          184 t
ORIGIN      
        1 ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt
       61 gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa
      121 aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt
      181 ctcaatgcaa gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg
      241 atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc
      301 atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa
      361 attttgattg atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt
      421 cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa
      481 aatcaaatcg aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcat
//
LOCUS       DADVXO010000314          535 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.329, whole genome shotgun sequence.
ACCESSION   DADVXO010000314 DADVXO010000000
VERSION     DADVXO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.329"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(38..>535)
                     /locus_tag="KB950_002945"
     CDS             complement(38..>535)
                     /locus_tag="KB950_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859079.1"
                     /translation="PLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYL
                     LLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVI
                     ITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEI
                     IDRIE"
BASE COUNT          170 a           94 c           87 g          184 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtgg
//
LOCUS       DADVXO010000315          534 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.330, whole genome shotgun sequence.
ACCESSION   DADVXO010000315 DADVXO010000000
VERSION     DADVXO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.330"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>534
                     /locus_tag="KB950_002946"
     CDS             <1..>534
                     /locus_tag="KB950_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBB6859080.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DADVXO010000316          514 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.331, whole genome shotgun sequence.
ACCESSION   DADVXO010000316 DADVXO010000000
VERSION     DADVXO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.331"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(107..>514)
                     /locus_tag="KB950_002947"
     CDS             complement(107..>514)
                     /locus_tag="KB950_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBB6859081.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DADVXO010000317          502 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.332, whole genome shotgun sequence.
ACCESSION   DADVXO010000317 DADVXO010000000
VERSION     DADVXO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.332"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            77..>502
                     /locus_tag="KB950_002948"
     CDS             77..>502
                     /locus_tag="KB950_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HBB6859082.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEK"
BASE COUNT          199 a           57 c           93 g          153 t
ORIGIN      
        1 ggtataatta gtatatgttt tgtgagaaat ttagacttac aataagcagt gaatttataa
       61 tcaacgtata ggagacatgg atgaaatgaa actaattggt atagatttag acggtactct
      121 tttgaattct gaccagagca ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga
      181 agactgtttt ccattcattt gttctggaag agaagtagaa gacattaata atattctcaa
      241 aaaatctggt ttaaggatac cagcagtagg gttaaatggt tctgttggat ttgatggaga
      301 aaaaaagata tttgagtctt ccttcgataa aaatgatgta aaaaaaattg attctgtcct
      361 atcgaaattt ccaaccaaaa tatatacaaa tctcggaagt tatgaatctc agaattataa
      421 aaataaaatg aaaaaaattt ttcacgaggt agggagtgaa ttttctatca aagagctaaa
      481 ctatgagctt gagtatgaaa aa
//
LOCUS       DADVXO010000318          501 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.333, whole genome shotgun sequence.
ACCESSION   DADVXO010000318 DADVXO010000000
VERSION     DADVXO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.333"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            222..497
                     /locus_tag="KB950_002949"
     CDS             222..497
                     /locus_tag="KB950_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6859083.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT          182 a           64 c           93 g          162 t
ORIGIN      
        1 cctcctaaaa ttagtatacc tcaaaaaagg ggtgttgtaa ataaatttta caaaactatt
       61 gacacccctt ttttatcgag gtattatgtg tatgtcgatg aactggtaat cattttttgt
      121 ttgattaatc ggttaataac aaaatacttt tattttgagc aaaaaatacg aagataattc
      181 ttagaaaatt tgttcaagca atcacgaaag gagggggaaa tatgtctcta caaatcattg
      241 atagcattgt ttctatacaa gttgaacaac aagtgaaaga gataattaca gtgctaaagc
      301 ctatgctaga agacattatt tctggagtca ctctagagta tatggactat cagcaggcaa
      361 gtgaatatct aggtgtatca gtaggaacga ttagaaaata tgtttctcaa tacgggttac
      421 ctgttataaa aattgatacg gtcattagat ttaaaaaatc tgatattgat agtttcatgg
      481 aacggtataa aggttgagta a
//
LOCUS       DADVXO010000319          487 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.335, whole genome shotgun sequence.
ACCESSION   DADVXO010000319 DADVXO010000000
VERSION     DADVXO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.335"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>487)
                     /locus_tag="KB950_002950"
     CDS             complement(<1..>487)
                     /locus_tag="KB950_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBB6859084.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DADVXO010000320          476 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.337, whole genome shotgun sequence.
ACCESSION   DADVXO010000320 DADVXO010000000
VERSION     DADVXO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.337"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            122..>476
                     /locus_tag="KB950_002951"
     CDS             122..>476
                     /locus_tag="KB950_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBB6859085.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGMDRLQLT"
BASE COUNT          158 a           98 c           96 g          124 t
ORIGIN      
        1 tttatataat aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa
       61 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      121 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      181 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      241 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
      301 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      361 ccgcaacggc gagttcaagc aacagactgt tcctgcttat agacggacga atgatacctt
      421 agaggagacc gtcattcacc tcttccgaaa aggtatggat aggctacaat taacta
//
LOCUS       DADVXO010000321          473 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.339, whole genome shotgun sequence.
ACCESSION   DADVXO010000321 DADVXO010000000
VERSION     DADVXO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.339"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(33..106)
                     /locus_tag="KB950_002952"
     tRNA            complement(33..106)
                     /locus_tag="KB950_002952"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Ile,seq:cat)
BASE COUNT          161 a           78 c           93 g          141 t
ORIGIN      
        1 ctaacacaga acgattcaat tggctacatt aatgtaccct gtaggactcg aacctacgac
       61 cggacggtta tgagccgtct gctctgacca actgagctaa gggtagtggt tgttgccaca
      121 taaagccata aacaatcaac cagtagaatg cgtggcaaca aacctgttat cgcatatctt
      181 ggagtgtgac tatttatgag tgatagtgaa gatatgcgtc tccatatatg tatggttgag
      241 acagataaac atgtatgaaa aacgttgtta aatcaacgtt tttttatttt ggtcaaggag
      301 agaaaagtag agaaatacaa aattttttgc attgattttg cattcaaaaa aacattcaaa
      361 catttagaaa tccccaatac gtattcagga aaatctattt taaggtttac aaaaagaact
      421 tttgttcgta cactcttttt gaggtgaact tttatgctga tggaaggaaa aac
//
LOCUS       DADVXO010000322          466 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.342, whole genome shotgun sequence.
ACCESSION   DADVXO010000322 DADVXO010000000
VERSION     DADVXO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.342"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            280..>466
                     /locus_tag="KB950_002953"
     CDS             280..>466
                     /locus_tag="KB950_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6859086.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVL"
BASE COUNT          171 a           74 c           66 g          155 t
ORIGIN      
        1 aggttttaga aaagatttat tatacgtaaa aagctcaaga cattttctta gccataagga
       61 catgtttgat atttaagaag atatatgaat tttcttttct aagtataaag ataactatcc
      121 attatcaact aaaaaaacat ttttgaaaat aaacatcagc agtcctatca atgcatgtga
      181 gcttatagaa gttattcgca gcgcagcata attgcattag cttcttttcg tgttttttgc
      241 ctcttttccc tttaaatctt aatggaggag gtgtgcaata tgtcagataa cgacgaacgc
      301 gtaaatgccc agttgattcg attctttgaa aaggtcattt tgaacaccgc taatacttat
      361 tataaaaaac aagaagaaat tcaaaaacat gaacaacttg atcaactccc accatatttg
      421 tttactaaag atactataaa atttaaacaa ccgattaatg ttttaa
//
LOCUS       DADVXO010000323          465 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.343, whole genome shotgun sequence.
ACCESSION   DADVXO010000323 DADVXO010000000
VERSION     DADVXO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.343"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            112..306
                     /locus_tag="KB950_002954"
     CDS             112..306
                     /locus_tag="KB950_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859087.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          198 a           60 c           77 g          130 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctc
//
LOCUS       DADVXO010000324          464 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.344, whole genome shotgun sequence.
ACCESSION   DADVXO010000324 DADVXO010000000
VERSION     DADVXO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.344"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>464
                     /locus_tag="KB950_002955"
     CDS             <1..>464
                     /locus_tag="KB950_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6859088.1"
                     /translation="LVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSG
                     NSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVT
                     MSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          168 a           85 c           77 g          134 t
ORIGIN      
        1 ctagtcacta aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac
       61 acactcctac ggactgaatt aacggctttt ttagattacg aaaaatatga tcgcactggt
      121 tttaattcag gtaattcgag aaacggttct tactttcgat caatcaaaac cgaatatggt
      181 gaattaacat tggaaatacc tagagatcgt aatggtgagt ttaaacaaca aactttacca
      241 gcctacaaaa gaacaaacga tacattggaa accactatta tccatttatt cgaaaaaggt
      301 gttacgatgt ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca
      361 caaaccatgt ccaacatgac taaagttctg actgaagaag taaatgcctt taaatccaga
      421 gccttaaatg ataagtatgt cgctattttt atggacgcta ctta
//
LOCUS       DADVXO010000325          456 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.345, whole genome shotgun sequence.
ACCESSION   DADVXO010000325 DADVXO010000000
VERSION     DADVXO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.345"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(119..418)
                     /locus_tag="KB950_002956"
     CDS             complement(119..418)
                     /locus_tag="KB950_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBB6859089.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          138 a           91 c           56 g          171 t
ORIGIN      
        1 gaaacatctg tttctttatt tagaataaaa aaatacagac tctgttcaaa taaatgaacc
       61 tagcctgcat tatgagattg actatacttt ttaattcatt gagatgaata actcgttttt
      121 ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg acctaattct ttttcaaatt
      181 ttcctattgt tgatatctcc atataaaacg gtgatactgg tttagacaat tctcccctga
      241 ttgctaacgg acgtaaacta cgttccaaat tatttatgac tttaggataa tactgacctt
      301 tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc cttttctttg atctctggat
      361 ttaaaacgag atcatataat tttagtacca tgttttctat ttcttcactt ttagacatca
      421 cgatttcctc ccaaaaatat atttcttccc actaca
//
LOCUS       DADVXO010000326          455 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.346, whole genome shotgun sequence.
ACCESSION   DADVXO010000326 DADVXO010000000
VERSION     DADVXO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.346"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>455
                     /locus_tag="KB950_002957"
     CDS             <1..>455
                     /locus_tag="KB950_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859090.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          184 a           67 c          106 g           98 t
ORIGIN      
        1 agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt accagaagat
       61 aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc agaggaagca
      121 actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat
      181 aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga attgaataag
      241 gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga tactaagtat
      301 acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac tgttgtagtg
      361 acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac agacaatcgt
      421 tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DADVXO010000327          453 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.347, whole genome shotgun sequence.
ACCESSION   DADVXO010000327 DADVXO010000000
VERSION     DADVXO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.347"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
BASE COUNT          147 a           88 c           51 g          167 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aat
//
LOCUS       DADVXO010000328          452 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.348, whole genome shotgun sequence.
ACCESSION   DADVXO010000328 DADVXO010000000
VERSION     DADVXO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.348"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..85
                     /locus_tag="KB950_002958"
     CDS             <1..85
                     /locus_tag="KB950_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6859091.1"
                     /translation="GKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            179..370
                     /locus_tag="KB950_002959"
     CDS             179..370
                     /locus_tag="KB950_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6859092.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          169 a           63 c           79 g          141 t
ORIGIN      
        1 tggaaaggtt ctggggatta caagacaggg tgcgacaaat ttaaaacatc gactttacaa
       61 aaaaattcaa aaaatgctgg tttaatatct gcataataac taattttgta tgcttctttg
      121 ctcgttctcc tccttcccaa agaaatagat tataagtcca tgggaagaag gtagatttat
      181 gaattcttta aaagaagatt tcattttagc aaaggctgga aatgaagaag ctgttgaggc
      241 aattctcaaa agatttagct ctttgattca taagcaatcc tggagaactg gaaaatatga
      301 tcaagattgt tatcaagaat gtatgcttgc aatatatcta gctatctcga aattcgaaat
      361 taaagaataa ctcaactaag ggagtctgtg acataagtaa aaagatagtt tcaaatttcg
      421 aacaataaat ttgaaactat cttctttata at
//
LOCUS       DADVXO010000329          440 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.349, whole genome shotgun sequence.
ACCESSION   DADVXO010000329 DADVXO010000000
VERSION     DADVXO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.349"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..159
                     /locus_tag="KB950_002960"
     CDS             <1..159
                     /locus_tag="KB950_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6859093.1"
                     /translation="SFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANE
                     VLKIAEEK"
     gene            160..>440
                     /locus_tag="KB950_002961"
     CDS             160..>440
                     /locus_tag="KB950_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBB6859094.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDS"
BASE COUNT          168 a           52 c           92 g          128 t
ORIGIN      
        1 agttttgaag aaaacattgt taaagaaatg cctgaatctg ctaatgatat gtatgagaag
       61 gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa tgatttatat
      121 catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt atttactatt
      181 tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag
      241 cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca tcctgaacat
      301 aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac gactaagtta
      361 aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac tgtagggaaa
      421 attgaagtga tacgtgattc
//
LOCUS       DADVXO010000330          438 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.350, whole genome shotgun sequence.
ACCESSION   DADVXO010000330 DADVXO010000000
VERSION     DADVXO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.350"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            9..374
                     /locus_tag="KB950_002962"
     CDS             9..374
                     /locus_tag="KB950_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859095.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          114 a           86 c           64 g          174 t
ORIGIN      
        1 gatgaaagat gataaaaact tgctggaaaa accttccgtt gcttttatct tttgttcctt
       61 atgtgcattt cgccttactg cttgattttc gttatcattc cgtaagcggg ttcatcacat
      121 tgatattttt atctcttttt gctggatatt attttcaaag gaacaggcgt attatatctt
      181 tattcattgc caatattata tctactgtca cttcctattt attttgcgcc aattttactg
      241 aatggcgtta cttctatcat cctttaaaac cgacccagct gatcttgctt cttgcaggta
      301 tttacttagt tccacaaatc cttgggtcgt tatgggcggt agccttatct tataaaaaag
      361 ctcggcatcc ataaaaacaa accatatatg tacaaagaga agctactttc ttctttttat
      421 acatatatgt ttttctta
//
LOCUS       DADVXO010000331          437 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.351, whole genome shotgun sequence.
ACCESSION   DADVXO010000331 DADVXO010000000
VERSION     DADVXO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.351"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>437
                     /locus_tag="KB950_002963"
     CDS             <1..>437
                     /locus_tag="KB950_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB6859096.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV"
BASE COUNT          136 a           82 c          105 g          114 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 acctttacac aagaacagcc attggaagcc atcgttacgg atcttactta tgttcgcgtg
      301 gggaaaaagt ggcattatat ctgcttaata cttgatttgt ttaatcgaga aattattggt
      361 tattcctgtg gtgagaagaa agatgcctca ttggtaaaag aagcctttgg acggataccg
      421 tattctttaa cagacgt
//
LOCUS       DADVXO010000332          414 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.353, whole genome shotgun sequence.
ACCESSION   DADVXO010000332 DADVXO010000000
VERSION     DADVXO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.353"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(109..>414)
                     /locus_tag="KB950_002964"
     CDS             complement(109..>414)
                     /locus_tag="KB950_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6859097.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DADVXO010000333          412 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.354, whole genome shotgun sequence.
ACCESSION   DADVXO010000333 DADVXO010000000
VERSION     DADVXO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.354"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(5..199)
                     /locus_tag="KB950_002965"
     CDS             complement(5..199)
                     /locus_tag="KB950_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859098.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(299..385)
                     /locus_tag="KB950_002966"
                     /pseudo
     CDS             complement(299..385)
                     /locus_tag="KB950_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          133 a           83 c           73 g          123 t
ORIGIN      
        1 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
       61 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      121 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      181 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      241 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      301 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      361 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ct
//
LOCUS       DADVXO010000334          400 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.355, whole genome shotgun sequence.
ACCESSION   DADVXO010000334 DADVXO010000000
VERSION     DADVXO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.355"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            123..341
                     /locus_tag="KB950_002967"
                     /pseudo
     CDS             123..341
                     /locus_tag="KB950_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          135 a           58 c           71 g          136 t
ORIGIN      
        1 atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga gttactttca
       61 atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt gatgaaattg
      121 ccattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat tggtctaatg
      181 gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa aataaagtta
      241 ttccagtatt gctatatgga acacataaca atttttatga attagccgag aacactcgca
      301 actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta tatcaatcaa
      361 ccgaccgaca tatgttaaat ctatatataa taaaaatacc
//
LOCUS       DADVXO010000335          400 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.356, whole genome shotgun sequence.
ACCESSION   DADVXO010000335 DADVXO010000000
VERSION     DADVXO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.356"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            202..>400
                     /locus_tag="KB950_002968"
     CDS             202..>400
                     /locus_tag="KB950_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859099.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYL"
BASE COUNT          137 a           61 c           71 g          131 t
ORIGIN      
        1 gagtctatta attcttgtag gattgctttt tgtatcagtt attctgctct ttctcattag
       61 tttagctcta aggaatgagt tcagtaataa acgttaacga ttgaaacttc aagtcctgtg
      121 taagataata aagttaattc atcttattag gaggaagtac ccatagaaac cagagattat
      181 attataactc accaaaagtt aatagaaagt ggactaagcc attttgaaaa agaaggattt
      241 cagcaaacta agataaggca actgtgcctg accgcaggcg taactacggg tgctttttat
      301 aaacatttta cttctaagga agcgttattt gaagaaatta ttcagccgca tattaatgcg
      361 cttattaata tgtataatga agagtttacc aaatacctct
//
LOCUS       DADVXO010000336          397 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.358, whole genome shotgun sequence.
ACCESSION   DADVXO010000336 DADVXO010000000
VERSION     DADVXO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.358"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>397)
                     /locus_tag="KB950_002969"
     CDS             complement(<1..>397)
                     /locus_tag="KB950_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859100.1"
                     /translation="TVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLP
                     YKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVA
                     DIKVSTSYTVTTIISYLVTLTAYLCLFFKI"
BASE COUNT          123 a           72 c           55 g          147 t
ORIGIN      
        1 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
       61 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
      121 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
      181 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
      241 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
      301 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
      361 ataatccatt cctttagtgg tatacgcctc tactgtt
//
LOCUS       DADVXO010000337          369 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.360, whole genome shotgun sequence.
ACCESSION   DADVXO010000337 DADVXO010000000
VERSION     DADVXO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.360"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            194..369
                     /locus_tag="KB950_002970"
                     /pseudo
     CDS             194..369
                     /locus_tag="KB950_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          138 a           45 c           66 g          120 t
ORIGIN      
        1 ttaaacttta tattataata atgaagctta atcttcttta tagagaattg ggctattttt
       61 aatggcaaag aggggaagaa gatgtttcaa aacaaaggac aacgctatgt tgtaaaagga
      121 gtaaaggaat cttactggaa tcaaaaatct ttattgagaa aattatcttt gaatttaagc
      181 acaaagaaat gaaggtacac tagatagcaa tgaagatatc tttgtcatta aacgtcgcta
      241 ttttacacaa gtaagtgatg atttgattca tgtcttttcc aatgagtggt ttgtttctga
      301 gaaaaaatta catgcttcta atctgcaata tatgccaggt gaagatccaa ttcctaatat
      361 gaaagcaat
//
LOCUS       DADVXO010000338          336 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.366, whole genome shotgun sequence.
ACCESSION   DADVXO010000338 DADVXO010000000
VERSION     DADVXO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.366"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..335
                     /locus_tag="KB950_002971"
     CDS             <1..335
                     /locus_tag="KB950_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBB6859101.1"
                     /translation="NRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRL
                     ESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQ
                     AFERILTI"
BASE COUNT          123 a           45 c           61 g          107 t
ORIGIN      
        1 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
       61 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      121 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
      181 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
      241 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
      301 tatctcaagc atttgaaaga attcttacca tataaa
//
LOCUS       DADVXO010000339          321 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.369, whole genome shotgun sequence.
ACCESSION   DADVXO010000339 DADVXO010000000
VERSION     DADVXO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.369"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(69..321)
                     /locus_tag="KB950_002972"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="KB950_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DADVXO010000340          318 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.370, whole genome shotgun sequence.
ACCESSION   DADVXO010000340 DADVXO010000000
VERSION     DADVXO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.370"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            121..>318
                     /locus_tag="KB950_002973"
     CDS             121..>318
                     /locus_tag="KB950_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6859102.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKN"
BASE COUNT          127 a           33 c           53 g          105 t
ORIGIN      
        1 cttggacaaa gttatttaat attaataagt attattgttt tatcagttgt atttgcttct
       61 tcattaatgt taatcaagaa gcctaaagaa atcttatcaa aaataagtta ggagaaaata
      121 atggatatat tagaaataaa gaatgtagca tatagttatg caaattctaa agaaaaagtt
      181 ttgtcaggag taaatcaaaa atttgaactt gggaagtttt atgcgatagt aggaaagtca
      241 ggaacaggaa aatctacact tctttcctta cttgctggac ttgataaacc tcaaacagga
      301 aaaatattgt ttaagaat
//
LOCUS       DADVXO010000341          314 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.371, whole genome shotgun sequence.
ACCESSION   DADVXO010000341 DADVXO010000000
VERSION     DADVXO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.371"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>314
                     /locus_tag="KB950_002974"
     CDS             <1..>314
                     /locus_tag="KB950_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859103.1"
                     /translation="SIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGK
                     KETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFE
                     KT"
BASE COUNT          105 a           45 c           50 g          114 t
ORIGIN      
        1 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
       61 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
      121 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
      181 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
      241 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
      301 tgtttgaaaa gacg
//
LOCUS       DADVXO010000342          303 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.373, whole genome shotgun sequence.
ACCESSION   DADVXO010000342 DADVXO010000000
VERSION     DADVXO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.373"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="KB950_002975"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="KB950_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBB6859104.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          113 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atg
//
LOCUS       DADVXO010000343          302 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.374, whole genome shotgun sequence.
ACCESSION   DADVXO010000343 DADVXO010000000
VERSION     DADVXO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.374"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            1..198
                     /locus_tag="KB950_002976"
     CDS             1..198
                     /locus_tag="KB950_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBB6859105.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            200..>302
                     /locus_tag="KB950_002977"
     CDS             200..>302
                     /locus_tag="KB950_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBB6859106.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKV"
BASE COUNT          112 a           34 c           61 g           95 t
ORIGIN      
        1 atgaaacatt taaaaatttt gtctattaaa gagacacaac ttatctatgg gggtaccact
       61 catagtggaa aatattatgg aaatggagtg tattgcacta aaaataaatg tacggtcgat
      121 tgggccaagg caactacttg tattgcagga atgtctatag gtggtttttt aggtggagca
      181 attccaggga agtgctaaaa tgaaaaaaaa tgctaagcaa attgttcatg aattatataa
      241 tgatatatct ataagtaaag atcctaaata ttctgatatt cttgaggttt tacaaaaggt
      301 at
//
LOCUS       DADVXO010000344          282 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.375, whole genome shotgun sequence.
ACCESSION   DADVXO010000344 DADVXO010000000
VERSION     DADVXO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.375"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>282
                     /locus_tag="KB950_002978"
     CDS             <1..>282
                     /locus_tag="KB950_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBB6859107.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DADVXO010000345          279 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.376, whole genome shotgun sequence.
ACCESSION   DADVXO010000345 DADVXO010000000
VERSION     DADVXO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.376"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            1..279
                     /locus_tag="KB950_002979"
                     /pseudo
     CDS             1..279
                     /locus_tag="KB950_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          107 a           56 c           55 g           61 t
ORIGIN      
        1 caaatcgtcg atctctatct cgctggtaag cctcgcgcag aaattattcg agagtatgag
       61 cttacgcctt cttctttcga taaatggatg aagcaagcac aatcaacggg ctcattcaaa
      121 gaaagagaca acttaacacc agaacaagca gaattgatcg cactaagaaa gaaaaataag
      181 caactcgaaa tggagaatga tattttaaag caagcggcgc tgatattcgg acgaaaagac
      241 aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DADVXO010000346          277 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.377, whole genome shotgun sequence.
ACCESSION   DADVXO010000346 DADVXO010000000
VERSION     DADVXO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.377"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>277)
                     /locus_tag="KB950_002980"
     CDS             complement(<1..>277)
                     /locus_tag="KB950_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6859108.1"
                     /translation="YKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETK
                     LHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAE"
BASE COUNT           84 a           49 c           30 g          114 t
ORIGIN      
        1 ttcagcttta gctaattttg taaatgttac tgttccatta taattttcta aaacaacttt
       61 ctctatttcg tctagtgaaa ctttgaaaaa ttcttttctt tgattaattt tattaacttc
      121 ttgttttcta aaatgattat gcaatttagt ttccaaagac ggagcgtcct ctgaaaaaat
      181 catcgcatga acgtcaaatt caaatggaac cgatgcacta ctcaattctt ttattctatc
      241 cataggttct aatcttctag tcataccaat tttatat
//
LOCUS       DADVXO010000347          267 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.380, whole genome shotgun sequence.
ACCESSION   DADVXO010000347 DADVXO010000000
VERSION     DADVXO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.380"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(35..>267)
                     /locus_tag="KB950_002981"
     CDS             complement(35..>267)
                     /locus_tag="KB950_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6859109.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          103 t
ORIGIN      
        1 tagaaggact gaccttctgt ctcagatttt tttgttattt cacttgaatc aattgattga
       61 tcaaaaaaat cctgattttc atgttctcaa atgatttaaa tccataagat aatcgtttca
      121 gtgtcttgat atggggaatc ttggcctcta ttttgccatt agaatacggg taaatcatag
      181 cattgtaaat accttcttca tacgttaaaa ggttttgaag tttcgtgcga agttgtgcat
      241 caagtgtttc aggaagattt tttaata
//
LOCUS       DADVXO010000348          253 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.384, whole genome shotgun sequence.
ACCESSION   DADVXO010000348 DADVXO010000000
VERSION     DADVXO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.384"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            28..>253
                     /locus_tag="KB950_002982"
     CDS             28..>253
                     /locus_tag="KB950_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBB6859110.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DADVXO010000349          251 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.denovo.385, whole genome shotgun sequence.
ACCESSION   DADVXO010000349 DADVXO010000000
VERSION     DADVXO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.denovo.385"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
BASE COUNT          101 a           38 c           42 g           70 t
ORIGIN      
        1 ttaaagcgta atacacttat ggggaagtgg tttggggtgc gctttaaact cttctttatt
       61 attatctcac agcatgctta aaaggtctat ggattaagtt atgtaaaaaa gtgcctcttt
      121 aaacgtccta aaatccaaac aaggatagca taatgtacac tatcgttgat ccagaaaaca
      181 ggtaaataaa ttttgcaaaa gcaataagtt aaaaacatta caaaaaagac aaggatatca
      241 catcaagaaa a
//
LOCUS       DADVXO010000350        28889 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.022, whole genome shotgun sequence.
ACCESSION   DADVXO010000350 DADVXO010000000
VERSION     DADVXO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.022"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..686)
                     /locus_tag="KB950_002983"
     CDS             complement(<1..686)
                     /locus_tag="KB950_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859111.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTW"
     gene            791..1036
                     /locus_tag="KB950_002984"
     CDS             791..1036
                     /locus_tag="KB950_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6859112.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1192..2043
                     /locus_tag="KB950_002985"
     CDS             1192..2043
                     /locus_tag="KB950_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBB6859113.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2076..2750
                     /locus_tag="KB950_002986"
                     /pseudo
     CDS             2076..2750
                     /locus_tag="KB950_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            3007..3195
                     /locus_tag="KB950_002987"
                     /pseudo
     CDS             3007..3195
                     /locus_tag="KB950_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3241..4206
                     /locus_tag="KB950_002988"
     CDS             3241..4206
                     /locus_tag="KB950_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6859114.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4228..4440
                     /locus_tag="KB950_002989"
     CDS             4228..4440
                     /locus_tag="KB950_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6859115.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            4515..4991
                     /locus_tag="KB950_002990"
     CDS             4515..4991
                     /locus_tag="KB950_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBB6859116.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4988..6796
                     /locus_tag="KB950_002991"
     CDS             4988..6796
                     /locus_tag="KB950_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBB6859117.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            6873..7088
                     /locus_tag="KB950_002992"
     CDS             6873..7088
                     /locus_tag="KB950_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBB6859118.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7180..8043
                     /locus_tag="KB950_002993"
     CDS             7180..8043
                     /locus_tag="KB950_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859119.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8080..8616
                     /locus_tag="KB950_002994"
     CDS             8080..8616
                     /locus_tag="KB950_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBB6859120.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            8630..9052
                     /locus_tag="KB950_002995"
     CDS             8630..9052
                     /locus_tag="KB950_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6859121.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            8982..11381
                     /locus_tag="KB950_002996"
     CDS             8982..11381
                     /locus_tag="KB950_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6859122.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11413..13404
                     /locus_tag="KB950_002997"
     CDS             11413..13404
                     /locus_tag="KB950_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6859123.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13427..13678
                     /locus_tag="KB950_002998"
     CDS             13427..13678
                     /locus_tag="KB950_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBB6859124.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            13668..14897
                     /locus_tag="KB950_002999"
     CDS             13668..14897
                     /locus_tag="KB950_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBB6859125.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            14894..16975
                     /locus_tag="KB950_003000"
     CDS             14894..16975
                     /locus_tag="KB950_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBB6859126.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17124..21044
                     /locus_tag="KB950_003001"
     CDS             17124..21044
                     /locus_tag="KB950_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBB6859127.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21045..21989
                     /locus_tag="KB950_003002"
     CDS             21045..21989
                     /locus_tag="KB950_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859128.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            21993..22196
                     /locus_tag="KB950_003003"
     CDS             21993..22196
                     /locus_tag="KB950_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBB6859129.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(22496..22732)
                     /locus_tag="KB950_003004"
     CDS             complement(22496..22732)
                     /locus_tag="KB950_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6859130.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(22915..23301)
                     /locus_tag="KB950_003005"
     CDS             complement(22915..23301)
                     /locus_tag="KB950_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBB6859131.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(23283..24611)
                     /locus_tag="KB950_003006"
     CDS             complement(23283..24611)
                     /locus_tag="KB950_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6859132.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(24572..24901)
                     /gene="mobC"
                     /locus_tag="KB950_003007"
     CDS             complement(24572..24901)
                     /gene="mobC"
                     /locus_tag="KB950_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBB6859133.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25159..25529)
                     /locus_tag="KB950_003008"
                     /pseudo
     CDS             complement(25159..25529)
                     /locus_tag="KB950_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            25674..25859
                     /locus_tag="KB950_003009"
     CDS             25674..25859
                     /locus_tag="KB950_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6859134.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26017..26184
                     /locus_tag="KB950_003010"
     CDS             26017..26184
                     /locus_tag="KB950_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859135.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            26802..27461
                     /gene="vanR"
                     /locus_tag="KB950_003011"
     CDS             26802..27461
                     /gene="vanR"
                     /locus_tag="KB950_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBB6859136.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27464..28825
                     /gene="vanS"
                     /locus_tag="KB950_003012"
     CDS             27464..28825
                     /gene="vanS"
                     /locus_tag="KB950_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBB6859137.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         7450 a         7338 c         7638 g         6463 t
ORIGIN      
        1 aaccatgttt tactttttga attgtagata aatctaaaat taatatcgtt attatcgtgt
       61 tgaaggggcg tctccaaata atctgctgta tattcatagt caacgtaacc tgctaataca
      121 aaagagagta tccctttatt taatgttaga tttaatggat aatcatacga ctttattttt
      181 ttcaacttca tattgtacat atcttcagcc ataaaagttg agttcgtgat ttcatcttgg
      241 gtctcattaa aaaagttatc cgaataatcg cttatttttg aaaagtcttt attggcacca
      301 gcctcaaaaa attgttcaac tttttcagta taaagttgtg ctaacgctcc tttttcaata
      361 ttaactttaa tattttcatg tctatcaatt tgtgtgctgt tcagtaaggg aagatcagct
      421 tttagtttta tatcgtcttg atttctttca atttcaattg gcaaaatctt ttcagtagat
      481 tcagtttctg ataaaggaac atctagtttt ttatcatgat aataagcttc aaatccatct
      541 ggaaccggaa aaaaatgtat tgggtaattc ttttctgagg ttgttgtact agtttccgta
      601 cttgtagaac ttgtgtcaga agtggtacac ccgcttatag ttccgatgag taaagcgcaa
      661 gttagaatga ttgcaaattt cttcaaaaaa attcctcctt aaaattgaat atggactaat
      721 actagtccca ttgtagcata ccgaatcttt aagattaata agaaaaagga ctaattttag
      781 tccaaaaggt gtgctattct tgaaagagga aaaaattatt gataaagaaa atgtaatcgg
      841 tagcaatatt agacgagtcc gattagaaaa aggaattggt caaaccgagc tgattagaga
      901 cctacagctt cgtaaaattg ctataactag ggaaacgttg gttaagattg aaggtggccg
      961 ccaacacatt aagttggatc aattgaaagc catcaaagat attctacagg tgtcttatga
     1021 agatttgctt caatgactac ttgtagtcat ttcgtttgaa attattatgt tacaccctta
     1081 tgtgtcacga agaaatactg gtataataat gaatgggata tgtaacgata gggtatactg
     1141 ttatgttgcc taataaattg tggatcaaaa tacaagaatg gagggatatc cttgtataag
     1201 gcatatgaat tggattttac aaatgctgac ttaaatgcat tctcctcaag ctcgcaatca
     1261 tatacacatg tagtaaatca aatagatcac aaccaaaaat ctattaacaa gagaatagaa
     1321 aatctatttg aaagtgaaaa tgatttagtt gattattatt ctaaggacag caaaattcta
     1381 gatgcagacg aaattattaa agattggttt ccaactataa aagcagatat ttttatatct
     1441 cactctcatg ctgatgaaaa attagctata cgttttgcat catggctatt tgaaaatttt
     1501 ggtcttactg catttattga ttcttcggtt tggggataca gttctgattt attaaaaaaa
     1561 atagaccaaa aatattgcta taaggaacaa acaaaaacat atgattatga taaaagaaat
     1621 gtaacaacta gtcatgttca tatgatgctt tctacagctt taaataatat gattgatagt
     1681 acagaatgtc tgttttttct taatacacct aattcaatta gtttgagcaa tgaaatcact
     1741 aatgaacaga aatttacgta ttcgccatgg ctatatagcg aactgacgac agcttcaata
     1801 gtggaaaaaa agaatccacg attggaaagt aatcctcaaa tgagcaccga ggatgttaga
     1861 agcattataa agcactattc ttcgcaagaa aatctgcaaa taaaatattc tttaaattta
     1921 agtgaattaa cttatttacg aaaagctgat ttgaaaaaat gggctttaga tgctgtggaa
     1981 tgcactaaag gaagtaatac attagatatt ttatataaaa acaccatttt cactaatctc
     2041 taatcaattt actgaatcga aaggaaccgg gtagaatgga taagaaagtt ttaataaaga
     2101 ggttctctga agctattgaa aaaggaaatg cagcaatatt tgcaggtgca ggattatcga
     2161 tgtcacaagg atatgtaagt tggccggaac tacttaatga tcctgcaaca gaaatcgggt
     2221 tggattctaa aaaagaaaca gacttagtta ctctggctca atattataaa aatgaaaatg
     2281 gtggatcaag aggaattttg aatcagatac ttatggataa ttttggtgaa gaattagaaa
     2341 tatctgaaaa tcatcgaata ctagcaagtt tacctattga aacttattgg acaactaatt
     2401 atgatcatct aatagaaaaa tcaatccgag aagcttataa aaaccctcag gttaagaaaa
     2461 attatactca gttagcaaca actaatccaa atgtggatac aattgtttat aaaatgcatg
     2521 gtgatataga tgatgtcagt tcgacagtaa taacaagaga tgattatgaa aaatatgatg
     2581 attcgtacgc gttatttaaa gaaactttaa aaggagattt gttaacaaaa acttttctct
     2641 ttttaggatt tagttttaca gaccctaatt tagaaagaat tttatcagat ataagatggg
     2701 ttttgagaga aaaccaaaga cctcattatt gtataatgag aaaaatatta tagaaaaaat
     2761 tttaggttat cgctgaaagc cccggaaata cgggcatttc gcaggatatg agaactcaat
     2821 ttactaccaa tttactactt tcggattgag ccgcatatat gctgaaccat actgctaata
     2881 cccgccaccc caaatacggc acatcataaa ttgaatacga caccgtaaac gcggcgttcc
     2941 tctttcccgt catgggagaa caggacgccg cttttttatg cccaaataga gaaaggaggc
     3001 gacgccatgc tatttacttc cggccctgac cgggcgtttg aacggctgat gcaggaaaag
     3061 cccggtttcg atcactacga cggcggcggg gcggacgcgc cggaggattg cggcacctgc
     3121 cgattttacc gcccggattg gaaatatcaa ttctgccagt ttgcagagtg tccctatcag
     3181 ccgggcaagc tgaccgacta tgcagcggtc aaattcacag tgaaaggagc gaacaacgct
     3241 atggcagttt tccgtgtgga aaagaacaaa ggctatacgg tgatgtcaaa tcaccacctg
     3301 cggaataaga acctgacctt aaaggccaag ggcctgctgt cgcagatgtt gtccttgccg
     3361 gaggattggg actataccct aaaaggactg tcctatatca accgggaaag catcgacgcg
     3421 atcaggacag cggtatggga gcttgaaaaa gctggatata tccggcgtga gcagggccgt
     3481 gacgcaaaag gcaaaatggc cgatatggtc tataccatct acgaacagcc ggtattggaa
     3541 aacccggtat tggaaaatcc aacatcggat aacccggtgt tggaaaatcc gacatcggat
     3601 aatccggcgt cggaaaatcc aacgcaatta aataaagaag tacaaagaac tgacttatca
     3661 aaaaaagaaa aaataattac agatggacaa agtacccatt ccattcctat cccatccccg
     3721 acccctgccc ctatggaggg cgaggcagcg gctacgccgc cggaacggaa aaggacagga
     3781 aaggatgagg ctgtacggat ttatcgggaa atcattttgg agaacatcga gtatgcctat
     3841 ctcattcagg acaggagcat tgaccgtgaa cagcttgacg aaatcgtaga cctgatgctg
     3901 gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg gggacgacta ccctgccgag
     3961 ctggtgaaat ccaagtacat gaagctgacc ggcgaacata tccgctttgt ccttgactgt
     4021 ctgcgggaga acacaacgaa agtccgcaat atcaaacaat acctgcgggc tatgctgttc
     4081 aacgccccca gcaccatttc caactactac acggcccttg tcgcgcacga catggcacag
     4141 cccgattggg gaaagcctaa atccggcctg ccggactatt cctgcggcga gggcgaaagc
     4201 ctgtaacgag aaaaggagga cgaccctatg aagcagggcg cattgatttt tgacgagcga
     4261 acagaccgtt acgacatccg ctttgatctg gccgactatt acggcggcct gcattgcggg
     4321 gagtgtttcg acgtgatgat cggcggcaaa tggaggccca cccgcattga aatggccgaa
     4381 aactggtatc ttgtcggcat ccgggccgac gacctttccg gcctgcgggt gcggatttga
     4441 cctatatcta cctgcggcgt ctggtggttc cgccttagac gccgcttttg actttctgac
     4501 aaggagggat gcgattgcag gaagaagtta ctcaaaagac cattgccctg tccatgaaaa
     4561 cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa aaagtacctg caacaccggg
     4621 ccaagggcaa aaacacgctg caccacggcc agcagagcct aaagcagctt aaaaagcatg
     4681 gggctgatct ctccaacatt gagatcaccg aggccaacat tggagcgttc aagccatgcg
     4741 ccaagaaata cggcgtggat tttaccttac gaaaagataa gaccacccag ccgccccact
     4801 acgttgtgat tttcaaggcc aaggatgcag ataaccttga tctggcgttt cgggagttta
     4861 ccgccaagac gctttccaag gagcagcggc cctctatccg caaggttttg tccgctgtga
     4921 agcaaaaggc ggcggcccag accaagcagc gggccaagga gaaaatcaag gaaagggggc
     4981 tggaactgtg aagcctgacg taaagaaatt gctgatcctg aacctcccgt atctgatttt
     5041 tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg gccgctggtg ctgacctgtc
     5101 ggggaagctc ctgcattttg cggatggttt tgcggcggct tttgccaacc cgctgccaag
     5161 cctgcacctg tttgacctgt gtatcggtat cgccggtgcc gtgatcgtgc ggctggtggt
     5221 gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc atggagtatg gcagcgcccg
     5281 ctgggggacg gccaaagaca tagcgcctta catcgacccc aagtttgaaa ataatatcct
     5341 gctcacccaa acggaacggc ttaccatgac cggcaggccc aaagacccca agacagcgcg
     5401 gaataaaaac gtgctggtga tcggtggcag cggttcaggg aaaacgagat ttttcgtgaa
     5461 acccaacctg atgcaatgct ttccgaccac ggactatccg acttcgtttg tggtgacaga
     5521 tccgaaaggc actttggttc tggaaaccgg caggatgttc cagcaggcgg gctaccgcat
     5581 taaaatcctg aataccatta acttttccaa gtccatgaag tacaacccct ttgtctacat
     5641 ccattcggaa aaggacgtgc taaagctggt aaataccctg atttccaaca ccaagggcga
     5701 gggggaaaag tcagcggagg atttttgggt gaagtcggaa cggttgtttt attcggccct
     5761 gatcggctat atctggtacg aggccccagc ggaggaaatg aacttctcaa cgctgctgga
     5821 aatgataaac gccagtgaag cccgcgagga cgacccggag tttcaaagcc ccgtcgatct
     5881 catgtttgag cgtttggagc agaaagaccc cgaccatttt gcggtgcgcc agtacaagaa
     5941 attcctgctg tccgcaggca agacccgttc ttccatcctg atttcatgcg gtgccagact
     6001 tgcgcccttt gacatccggg aggtacgcga gctgatggag gacgacgaaa tggagctgga
     6061 caccatcggg gacgaaaaga cggtgctgta tctcattatg agcgatacgg acacgacctt
     6121 taatttcatt ctggctatgc tccaaagcca gctaatcaac ctgctttgtg accgggctga
     6181 tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg attttggatg aatttgccaa
     6241 cattggccaa atacccaatt tcgataaact cattgccacc attcgaagcc gtgaaatatc
     6301 ggcctctatt attttgcaga gccagtcgca gctaaaagcg atttacaagg acgccgccga
     6361 gatcatttcg gataactgcg atagtgtgct tttccttagt gggcgcggca aaaatgccaa
     6421 ggagatttcc gatgcgctgg ggaaagaaac gattgacagt ttcaacacca gcgagaaccg
     6481 gggaagccaa acgtcccacg gattgaacta tcaaaaatta ggaaaggagt tgatgtccca
     6541 agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg cagctacggg gtgtgcgccc
     6601 gtttttatcg gagaaattcg atattaccaa gcatccccgg tacaaatacc ttgccgacgc
     6661 cgacaagaag aatacctttg acgtggacag atttttgact atcacgcgcc ggaaacggca
     6721 gcaagtggta acgcaggatg aagtattcga cctgtatgaa atcgacctgt cggacgagga
     6781 cgccgccgct gaataaacag cggctttttt cattccaaca acaaaatcaa aaaatcatta
     6841 tgagccggaa accggcagaa agaggtaaat ttatggcatt ttttaattcc gcagttacta
     6901 ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg cgtatggggc ggcatcaatc
     6961 tgttggaggg atacgggcag gacaatccgg gggcaaaatc gcagggtgtc aagcagcttg
     7021 tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt tcccctgctg tccgggctgc
     7081 tgggctgatc ctggcctttg tccctgtcca aacccttaac taatcgcctc cgcgtcctcc
     7141 cggccagacc gggagggcgc gggaaaggag gtgctacccg tgattagtga aatcattgaa
     7201 gaatggataa aaggcatcct catggacggt atcacgggca acctgtccgg gctgttcgat
     7261 acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg gacgaacccc gcaggcatgg
     7321 aacagcggta ttttcaatat gctgcacagc ctgtcggaaa ccgtgatcgt ccccatagcc
     7381 ggggccatac tcgcccttgt catgtgctat gagcttatcc agatgataac ggaaaagaac
     7441 aatatgcacg actttgacag ctccatgttc ttccgctgga tattcaaatc cgcgtttgca
     7501 atcctgatcg taacgaacac atggaacatc gtcatgggcg tattcgacgc cacccaaagc
     7561 gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat ccattgattt taacaccctg
     7621 ataccagacc ttgaaagcca attggaggct atgagcgtgg gcgctctttt ggggctgtgg
     7681 ttccaaaccc ttgttgtggg cctcaccatg aatatccttt ccatctgcat tttccttgtg
     7741 acctatggcc gcatggtgga aatctatgtt gtcacgtcgc tggggcctat cccccttgcc
     7801 acaatgggca acgcggaatg gcgcggcatg ggccaagggt atctgaaatc cctgctggcg
     7861 ctgggcttcc aagccttttt aatcatggtt gtggtgggta tctacgcggt gctgatacag
     7921 aatatcggtt ccgcttccga catatccggc gcaatctggg gctgtatggg ctatacggtg
     7981 ctgctgtgct tctgcctgtt caagaccggc agtatatcaa aggccgtatt cggggcgcat
     8041 tgatcccaaa atccaagcat acacaggagg aacttgttta tttcaacgta caacatcatt
     8101 tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac agggcgcagc ggaaaatcac
     8161 tattccacca tgagcattga cgagctgtgc gccctgccgg tggaacggct ggcgtccaaa
     8221 gactgcctgc tgtttttgtg ggccacattc cccatgctgc cggaagctct gcggttaatc
     8281 aaagcgtggg gcttttcatt caagaccgtg gcttttgtat ggctgaaaca gaaccgtaaa
     8341 agccttacat ggttttacgg gctgggccgc tggacaaggg gaaacgcgga aatctgcctg
     8401 ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg tccatcagtt tattatcagc
     8461 ccgattgaac aacacagcaa aaagccggat gtgacccgtg aaaaaatcgt agagctggcg
     8521 ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa cccccggctg ggacgtgtgg
     8581 gggaacgagg tggaatgtga ccttatcctg acgtgaaagg aggtgccaaa tggcttatgt
     8641 aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt gtgtttgggc ttaccaaacg
     8701 gcaactgatc tgtttttccg gggcgctggt gatcggcgtc cccttatatt ttctcacccg
     8761 tgactatctt accaacagcg cggcggccct gctcatggta tttgccatgc tgccgggctt
     8821 tttgcttgcc atgtacgagc gtcacggcca gccccttgaa gtggtgatta tgcagatcat
     8881 ccagtgctgc ttcatccagc caaaggagcg tccctatcag accgacaaca cctacaccgc
     8941 ccttgtgcgg caataccaga tggaacagga ggtaaaggcc attgtccaaa aaggaaatcc
     9001 caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg aaaagaaatt gacgcggtaa
     9061 tccgtaaata taagggcgac ggcaggcccc acacggcgca gcagagcatc ccctacgagg
     9121 tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt cttttccaag tgcattgagt
     9181 ttgcggacat cagctatcag cttgcccagc cggacacgca gacggctatc tttgaaaaac
     9241 tgtgcgacct gtataactat gtggacgcct ccattcatat ccagttttct ttcctcaatc
     9301 ggaaagttga cccggtgcag tacgccaaga gctttgagat cgcgccgcag ggggacgact
     9361 tcgacgacat ccgggccgag tacaccggca ttctgcaaaa gcagcttgca aacggcaaca
     9421 acggcatggt aaagaccaaa tacctgacat tcaccatcga ggccgaaagc gtgaaagcgg
     9481 cgcgagccag attgaagcgc atcggctttg acctgttagg ctatttcaag agcatggggg
     9541 ccgtagccca tgtgatggac ggctgggaac ggctgaacct gctgcatggc gtgtaccacc
     9601 cggacggcga gatattcaat tttgactgga agtggctggc cccgtccggc ctctccacca
     9661 aggattttat tgccccgtcc tccctgtgct tcggcaatgc aaagacattc ggcatgggcg
     9721 gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc ggagctgtcg gacgatatgc
     9781 tggccgactt cctcaatacg gaaagcggtg ttttagtcaa tctccatgtg caggccattg
     9841 aacagaccaa ggcgatcaag accatcaagc gcaaaattac cgaccttgac gctatgaaga
     9901 ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat ggacattctt cccagcgacc
     9961 ttgccaccta cggcgaggac gccaagaagc tgctgacaaa gttgcagacc cgcaatgaac
    10021 ggctgttcca gttgacgttc cttgtcctca acgtggccga caccaagcaa aagctgaata
    10081 acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa ctgtcccctt gtccggctgg
    10141 actaccagca ggaacagggc cttgcaagca gcctgccgct gggcgtcaat cagatcaaga
    10201 tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt cccctttgtg acgcaggagc
    10261 tttttcaggg cggcgcggcc atgtattacg gcatcaacgc caagtcccgc aacatgatta
    10321 tgctggacag gaagcaggcc cgttgcccca atgccttgaa gctcggcacc cccggcagcg
    10381 gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt cttaacgacg cctgacgata
    10441 tttttatttc agacccggag gccgaatatt acccccttgt caaacggctc cacggacagg
    10501 taatccggct gtcgcctacc agcaaggact ttgtgaaccc gctggacatc aacctgaatt
    10561 attcggagga cgataacccg ctggccctga aatccgactt tgtgctgtcg ttctgtgagc
    10621 ttgtcatggg cggcaagaac gggctggaag ccattgaaaa gaccgtgata gaccgggctg
    10681 tgcgcgtgat ctaccggccc tatctggcag acccccgccc ggagaatatg ccgatcctgt
    10741 ccgacctcca caaagcgttg ttagatcagc atgtcccgga ggctgaccgg gtggcgcagg
    10801 cccttgacct gtatgtgtcc ggctccctga acgtatttaa ccaccgtacc aacgtggaca
    10861 tcggcaaccg gcttgtgtcc tttgacatta aggagctggg caagcagctc aaaaagctgg
    10921 ggatgctcat tgtccaagac cagatatggg ggcgcgtcac ggccaaccga agccaaggaa
    10981 aggccacatg gtattttgcc gacgagttcc atttgctttt gaaagaggaa cagacggcgg
    11041 cctattctgc ggagatttgg aagcggttta ggaaatgggg cggcattcca acaggtgcca
    11101 cccagaacgt caaagacctg ctttcctccc cggaaattga aaatatcctt gaaaacagcg
    11161 acttcattac gctgctcaat caggcgtccg gggatagaaa aatccttgcg gaacgcttga
    11221 atttgtccac cgaacagcag aagtacatcg acaattccga acccggcgag ggccttttga
    11281 tctttgaaaa cgtggtgctg ccctttacca accccatccc gcacaacacc cagctttaca
    11341 agattatgac gacccgctta aacgaggtgg cgggagtatg acccggcgac gccacaaagg
    11401 aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag tgacgcaggc catgacccgc
    11461 gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg agaacatttc cagccgggag
    11521 gccgaacagg atttgtccgc gccggggccg ggcggcaccg ccgataagct gctggaacgg
    11581 gccgacgcca tccatgacgg ccataaggcc aagaaagccg cgaagcaggc cggggctgtg
    11641 gccgccgagg gggacgctgc cctgcaccgg ccatccgccc ggctccaact gtcggaggaa
    11701 gaacgggccg acccggattt acagccctat atccgaaaat cggacaaacg ggccgataag
    11761 ctggataagg cccgcgccgc catccccaag aagcgcgtcc cggtcaagga aaccgtcttt
    11821 gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg acaaacagga caaggggcct
    11881 ccctccctga aacccaatcc ggcaggccgt cctgtttccg aggtttttct attcggccac
    11941 gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg agggcggcca taagggggag
    12001 gaacttgccg agcggcaggc cggaaaagcc atccgcagca ccatccggca ccacaggctg
    12061 aaaccctact gggacgcgga aaaggccgag cgcaggctat tgtctgccaa cgcggagtat
    12121 ttctaccaga aatctttgcg ggataacccg gagatcgcac aggcggccag caaccccatt
    12181 tcccggttct ggcagaaaca gcagatcaag cgccagtacg ccaaggccgc ccggacagcg
    12241 ggccagaccg caggcgcggc ggcacagggc gcggccaaga cagccggggc ctccacgaaa
    12301 gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct cctttgtggc ccgccactgg
    12361 aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc tgcttatcat cggcggctta
    12421 cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca tcacggcgtc ctcttacttt
    12481 tccgaggata gcgatatgtt ggccgccgag gacgcctacg cggagctgga agccgagctg
    12541 caagactatc tggacaacta cgaggccacc catgactatg acgaatacca ttttgacctt
    12601 gacgagatca gccatgaccc ctatgtgctg atttccattc tgtcggcgct ccatgacggg
    12661 atatttacca tcggggaggt acagggtgat ttgtccatgc tctttgacaa gcaatatatc
    12721 ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg aaaccgatac atggacggac
    12781 gcggagggca acacccatac agacacctac caagtgccgt atgactatta catctgctac
    12841 gtcacgctgg aaaacttcga cctgtcccat gtgccagtct atatcatgga cgaggaaacg
    12901 ctaagtttct attccctgta tatgtccacg ctaggaaacc ggcctgatct gttttccggc
    12961 agccagtacc ccaacgcctc caccctgacg ccgccgacct actacgacgt acccccggag
    13021 gctttggagg atgccacctt tgccgccatg ctcaaagagg cggaaaaata tatcggttat
    13081 ccctatgtgt ggggcggcag ctctcccagc accagctttg attgcagcgg cttcatttcg
    13141 tgggtaatca atcatagtgg atggaacgtg ggacggctgg gggcgcaggg cctatataac
    13201 atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg accttgtatt tttcaaggga
    13261 acctacgaca cccccggcgt gtcccacgtc ggcctgtatg tgggcaacaa tgtgatgctg
    13321 cattgcggga acccgatttc ctacacgaac cttacttcaa actactggca atcccatttt
    13381 tacagttacg ggcgtttacc ttaacgccag aaaggagctt tcaattatgg caaccaaact
    13441 tgaccgcatc gaaaaggaca tccagaaaac caagtctaag atcgcggagt ttcagaaaca
    13501 gcttcgggag ctggaaacgc agaaaaccga acaggagaat ttgcagatca tccagcttgt
    13561 gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc ttacgcagcg gcgcaatgca
    13621 ggccgcccct gccgcaaccc cttaccatga acaggaggac aatgcccatg aagaataaga
    13681 ccagacggat ttcccgtatt ctccccgcac ttctggccgt gatgctctgc gtcacggcct
    13741 ttcccgttac cgcccttgca ggcggcaatg acccggcacc ggccccgctg ccggaggcta
    13801 caaccgaacc cgctacgggc ggtttggagc ctgaaacaga cgaaaccggc accgccacgg
    13861 acgggacaga aaccgaggcc gaaaaagata ccggccctct gaccggcaag gacatcaccg
    13921 acctgttttc cgcgctgttc ggttccaagg tgagcattgc cgccacggac gacggcatcc
    13981 agatcaccac cggcaccgac aaaaaagaac ctgaacagac cggcaccgtc accaccaacg
    14041 gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa gaccgcgttc acccagcttc
    14101 ccaatgggac gacggttgag gtagtcggca cagacggcga ctggattaaa attctcttgc
    14161 cggagcgcat cggctatgtc cattccgact atatgaccgt cagcgaaaag gaagtagcgg
    14221 ctaccggcga gggcggtttt tccctctcca ttgacccgga ggaaattgct tctctgctgg
    14281 aactgttcaa tggcggcggg gccagcgccg ccctgtcccc ggacgggaac ctgtcactga
    14341 ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt tatcaccgtg gaaaccaaaa
    14401 acggcaatgt gttttatctg attattgacc gggacgacga gggaaaagaa actgtccatt
    14461 tcttaaatca ggtggatgaa gccgatctgc tgacgctgat ggaggacggg gaaaccgtca
    14521 cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc cggggctgtg aacacgaatt
    14581 gcccggtatg taaaaccaac ctgaccgagt gcagcggccc ggagccgcag gaaccccagc
    14641 cggaggaacc cgaagccccg gaggaagaac ccaagggcgg cgcgggcggc ctgatcgtgt
    14701 tcctgctggt ggcgctggcg ggcggcggtg cggcccttta ctatttcaag ttcaaaaagc
    14761 ccaaggctga aaccaccggc agcgacgatt tgggcgagta tgattttggg gaggacgagg
    14821 atttggagga cgaggaaccc gaaaccgagt ttgacccgga agctgatttt gtgagtgaac
    14881 aggaggacga ggaatgacat ataaacttgt aatcgcggaa aaacccagcg tggcccgcag
    14941 cattgcgggc gtgatcgggg ccacggagaa acacgacggc tatttggagg ggaacggcta
    15001 tctggtgagc tggtgcatcg gacatctggt gtcctttgcc gacgccggac gctatgacga
    15061 gcgtttcaaa aaatggcgct atgaggattt gcccattctc ccggaaacat ggcagtacat
    15121 cgtccccgac gacaagaaac agcagtttga cgttctctgt tcgctgatgc agcgccccga
    15181 cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag ggggaattga ttttccgttt
    15241 cgtctaccag atggcgggat gccaaaagcc ctttgagcgc ctttggattt cctctatgga
    15301 ggacagtgct attaaggacg gctttgccca cctgaaaccg ggcggcgact atgatccgct
    15361 gtatcaatcg gccctgtgcc gggccaaggc cgactggctg atcggcatta acgccacccg
    15421 gcttttttcc gtcctgtacc acaagaacct gactgtgggc cgcgtccaga cgccgacctt
    15481 aaaaatgctg gtggacaggg acgccaagat taccgatttc aagaaagaga aatatcacat
    15541 cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc cgcttttccg acgctgccga
    15601 ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc gtttgcgttt ccgtgacgcg
    15661 ggagaaaaag acggaacagc cgcccaagct ctatgacctt accaccttgc agcgggaggc
    15721 taaccggctt ttcggctata ccgccaagca gacccttgac tatgcccagg cactgtatct
    15781 aaagaagctg ctgacctatc cgagaacgga tagtcaatat cttaccgacg atatgctgcc
    15841 cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg ccgtttgcca agggcctgaa
    15901 cctttccccg cagtttgacc gcatcctgaa cagcaaaaag gtgtccgacc accacgccat
    15961 tatccccacc gctgaatttg tgaagcaggg ctttggcact ctggccgaga gtgaaaaaaa
    16021 gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg gccgggccgc atatctatga
    16081 ggccgtcacc gccacattta cctgtgccgg gcaggagttt accgccaagg gcaagaccgt
    16141 cctttccccc ggctggaaag agattgaacg ccggttccgt tcttccttaa agaccgacgc
    16201 cgacgaggac gccgaggcgg tgcgggagct gccggagctt tgcgagggcc agacctttgc
    16261 agacgtggcc gcctctgtca ccgagcattt caccacgccg cccaagccct acaccgaaga
    16321 tacgcttttg tccgctatgg aacgggccgg ggccgaggat atgccggagg atgccgagcg
    16381 caagggatta ggcacaccgg ccacccgcgc cgccattctg gaaaagctgg tgcagatggg
    16441 ctttgtccag agaaaaggca agcagctcat tcccaccaag gacggcatca atctggctgt
    16501 ggtgctgccg gaggcgctta cttctcccca gcttactgcc gaatgggaaa accggctgac
    16561 cgagatcgcc aaggggcagg ccgatcctga cgaatttatg gacggcatcg agacgcaggc
    16621 gcgggagctg gtgcagacct attcctgtat cagtgaggaa aagcagaaat tgtttcaggc
    16681 cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag gctgtctatg agggcaagaa
    16741 aaactactac tgcggaaacc gggcctgcca gttcgtcatg tggaagaatg accgattttt
    16801 tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct gcgctgctca aaaccggcaa
    16861 agtcaaggta aagggcctct attccgtaaa gaccggcaaa acctacgacg ggacggtgct
    16921 tttggctgat accgggggca agtacgtcaa ctaccgcatt gaacacagga gctaaaggac
    16981 agtaacacag caagcatagg gagtgtggct acactcccgg taaaatcccc cggccgcgtc
    17041 cctgccgggc cggggatttt tgcttgttgg ggaagtcccc aaccccctat tttttgaaac
    17101 agaacagaga aaggagtgac cgcgtgaacg aagtattttc tatccaagtc cataaccggc
    17161 agtttttgga acagggcaaa caaggcgtct ggctggattt gcccaccaca accgaaacgc
    17221 tgcaagccgc cctgcgggat gttggcatta ctgccgacaa tccgcaggat ttttttatca
    17281 acgggtattc ctgcccggag gacagacacc ttgccattcc ctatgacatg gtgctggccg
    17341 ccgacgtgga cgagctgaat ttccttgccg cccggctggg ccagcttgac gccgccgaga
    17401 tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg ctttgagaat atcggccaga
    17461 tcatcgacta caccgaaaat gtggattact atgtgcatct tcccgacgtt aggggggatg
    17521 gacagttggg cgactactac ctgaaccgtt ccggcatggt ggatatgccg gaggaatgga
    17581 aagccgggat tgatacggcg cagtttggac gccacattgc ccagcaggaa caaggcgttt
    17641 ttacccagta cggctatctg gtgagaagcg gcgacgaatg gcagcgcgtc catgagggcc
    17701 agccggtgcc ggaggaatac cgggtgatgc actaccccca gccggaagct gaccgggagg
    17761 aaccgcaggc ccagcagccg gaggcatccg aaagccccga caaagcgccg cccccggtta
    17821 tcccgatcat gctggacggc atgaacagca aagaacgcat gaaagtgatt actgaccgtc
    17881 tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta tatggcttac ttatccgcta
    17941 tgtccaagat gcacgattac agtctgaaca atacgctgct gatcgccatg cagggcggtt
    18001 cgattgtggc gggctataac aggtggcagg atgaatttga ccgccatgtg aagcggggcg
    18061 agaaaggcat taagatactg gccccggccc cgttcaaggt taagcagcag atggagaaaa
    18121 ccgacccggc cacccagcag cccgtgatcg gcgcggacgg caagcccgtg accgaagaac
    18181 aggaagtgac ggttccggcc tttaaggtgg tgacagtctt tgacatcagc cagaccgagg
    18241 gcaaggattt tcccatgccc tatatcagtg aacttaccgg cgacgtgcag cagtatcagg
    18301 atttctttgc cgcgctggaa cggacatcac cctatcccat aagcattgag ccgatttcag
    18361 acggggccaa gggccgctgc ttctacgcaa aacagcgcat tgccatcaac gagggcatga
    18421 gcgaattgca gaccctaaag accgccatcc atgagattgc ccatgccaag ctccacgcca
    18481 ttgaccggga ggcccccgcc gaggaacagg cggcccggcc agaccgcagc acccgcgagg
    18541 tacaggccga aagcgtggcc tataccgtct gccagtatta cgggctggac acgtcggact
    18601 attcttttgg ctatgtggcc gattggagca gcggcaggga gttatccgag ctgaaagcct
    18661 cccttgaaac catccgggcc acggccagcg aattgataac cgagatcgac ggccattttg
    18721 cccagcttca gcaggagcgc gaggcccggc aggaacagga ggccgtcacg gttgacgtta
    18781 ccccggaggc agcggccccg gcgcaggccg aggaagaaca ggccccggcc cttgatccgg
    18841 cagccgagcc gcttgtgact gtgctatgga gcgaaagccc ccagctaaag gacggccagc
    18901 aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct ggatgcagca tatccggcag
    18961 aggcaggcta tgacaaaacc gcgttccgca ttgactgtat ctttcagggc gagccggaca
    19021 gctacgaggg gcggcaggat tttggagata gggacggttc cctgatcgaa catatccaga
    19081 gctaccacca attttacgcg caggacgaaa actggaaaaa gcatgtaatc gctacaaccg
    19141 gcatagaggg ctgggagcag gacaaggccc atcgggatat gctgttaggc gagtttgtcc
    19201 cctatatgaa gctgcattgc agccttgccc aaatggaaca gacggcgcag ggccgcttac
    19261 agtccgggga aaccctgacg ccggaggaaa cggcctattt gggggcgctt ctctcttatg
    19321 tgcaggagtg ccgccccatg ctcaatcagg gccagtatca gttgccggag ccgccgaaac
    19381 tggctgactt cgaccagagc ttgcaggact acaaagcaca ggtacaggcc gagatcgcgc
    19441 aggaggccgc cgcagccggt atgaccgtgg aggaatacgc ggcggctggc tatgaagcac
    19501 cccaacagga caccttttcc atctaccagc ttcgaggcgg ggaggaaacc cgcgactatc
    19561 ggtttgagcc atacgagcgt ttacaggccg cagggctggc cgtggacagt gccaactatg
    19621 accttgttta tacggcaccg ctggataaaa gcacgtcttt ggaggatatt taccgcacat
    19681 tcaacatcga ccacccggca gattttaggg gacacagcct ttccgtttcc gacgtggtgg
    19741 tgctgcacca gaatggacag gacaccgccc actatgtaga cagcttcggc tatatggaag
    19801 tgccggagtt tttgcgggaa aatcccctgc gtaccgccga actgtctacg gagcagaacg
    19861 aaaacatgat tgacggcatt atcaacaata cgccgcccga accgcagccg gaggaacccg
    19921 aacagctatc ttttgaggat gtggcagttg cttccggcca gacacaggag gaacccccgg
    19981 aggctgcgcc cccgcaaacg gctgtccgct attatcccat caatgaggga gccgcccgcc
    20041 gcgccaagga agccatgagc ttttctgact accggcccgg cagcgccacg gcggaatacc
    20101 ggcactatgt cgatcaggcc gtggaaattg cggagcgcca gaaaaagcgt gtggagccag
    20161 agtatcacga aaagatagac agcctccttg acacctacgc ccgcaagctg gccgagaaca
    20221 tgaataaggg ctatgagatt accgcccgcg tcccctctgt cctgattgcc gggccgtcca
    20281 atttcccggt acgggcgaaa gaaaagcaga acgccgccag tgaccgcaac atggaggaat
    20341 ttcagtacat tcagggcttg cttgacaaaa tccgcagcac cggcatgggt ggtatcagcg
    20401 ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact ggaaaagctg gaagcgtcgc
    20461 aggagttgat gaaggccgtc aatgcctact accgcaagca tgggacgctg gacggatgcc
    20521 cccatctgat cgaaagaggc attgaaaacc tgaaagcgga tatggcgtcg ggctggcact
    20581 atgagaaaaa accgttccag tcctggcagc tctccaacaa taacgccgag atacgccggt
    20641 taaagggccg cattgaggaa ttgacccgcc aaaaggaggc ggcctatgtg ggatgggagt
    20701 ttgacggcgg cactgtggag atcaaccggg tggccaaccg cctgcaaatc ttctttgagg
    20761 ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa cggtttccgc tggtcgccca
    20821 aggccggggc atggcagcgg cagttaaacg acaccacaat ccgcgtcgct gaccgtatca
    20881 agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt acagcttgcg gcccgcaggg
    20941 agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc caagaccgaa caggccaagc
    21001 agccaccggc gcgggaaaaa tcaaagggat tggaggtaga gtaaatgcag aagtttgaaa
    21061 atgtggacgt tctggccgcg ctggaacaga tcatgcggca gaacaccgcg ttttatcaaa
    21121 gtgactttgg cattgacaaa gacattatca ggcgggatgc cgccagcgat caggccgcag
    21181 acaagaccct gctctggatg tcccggccct ccgggacgta ctgtttccgg gaacgtgatg
    21241 ttttcctgaa agacacccga cagtacaaca catggaaatt ttacggggag cagacccgtg
    21301 acaagatact ggcctacgcg gtggagctga ccggcacaca gggcggcact atccggggca
    21361 atctctatga gctggactat ccgcagcatt tccggcacgt cattgaaacc gcccagccgg
    21421 tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga catcccggag agccagcgtt
    21481 ttgacggaag ccctgaccgg gtattgggaa actttttgta ctatgaggcc cagccccatg
    21541 acccggctgt gctgcaggag gcgctacgga cggagcagca cagccggggg cgggccgtcc
    21601 ccggcgattt caaagtccac gtcaccgctt tgcatgacag ccggattgaa accgaggccc
    21661 ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa cagccccaat aaaacgcatt
    21721 tcatggtgga gatctccccg cactttgcgg cgattgcttc caccaaggat aatgaccggc
    21781 tctttgccat gctgccctat aaatccctgt gttttaccgg gatgaaaggc aggcatggcg
    21841 tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag tattcgcagg ccccggccct
    21901 ctatccgcaa gcagctatcc gacagcaaac aggccgccag cccaaaaaag gcggcggcca
    21961 gaaccaaaaa gaatgaattg gaggtataag gaatgatgca ctttaccgtg gaggaagaaa
    22021 acctgatctg tatgtaccac aatgcagacc ggcgcagaac gataaacagg ctcaccgccg
    22081 tcatgccggt tatggatggg gatatgcggg cgcaggccca gcagaccctt tccaagctgg
    22141 aacgcatgac ggatgccgac tatgacggcc agaagttcca cttcacagac gaataacgaa
    22201 cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt ccggcgtctt ttctttttgc
    22261 ctattcctac gcatttagaa cccggtattg tcggggatag gatgaagtat tcgcttgccc
    22321 ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc aatcccttaa atgcgtaggt
    22381 tgcagctata attagacccc tgaaacagat atagccgcgc agcgggtgta tctgtttcag
    22441 gggtgtgcag gtgatagccg caaagcggcg taagagggtg cagccctctt gccgtctatc
    22501 tctgttccag cgggaagctc ggcaatgctt ccagcagttt cagggaaatg cgctgccgta
    22561 tatcttcgtc aatgactttc ttctttttgc cgtttgcctg tctgatctcc accgtggaaa
    22621 gctcgttgat gtagtcagca taatgggcca acactttttc cgtggcccac ctctcaccgg
    22681 caacggcggc tttgatcgtt tcatagtcca gcagcttttc ttgtccgtcc atcctgatac
    22741 ccccgtcaga ctgatttttt ctatgatacc atattcggaa gcatggaacc agagaaaacg
    22801 gcgcttttct gctggtaaat tctacgcatt tagaacagca cttcggcggg gataggataa
    22861 aacacccaca taccccgctg accatgccgg aaaagccttg aaatataagg gcttttacag
    22921 ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc tcggcctctt tgcggcggcg
    22981 catccgtttg gagcaggccg cgcagtattt ggcccggtta gacttcggcg tgaacgtggc
    23041 cccgcactca cagcagcgtt tcacttcgtc ccggtctttg gtgatctcgg catacagggc
    23101 cgcatccagc gggaggacag cggccttaaa ccagcggcac agaagcgaat aggaaatgct
    23161 ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg cagttgccgt cacaatagtt
    23221 gcagcactca tgcgtcagct tccgggcctt gcgatattga gggtaattca ttcgtggaat
    23281 actcatagct cttgcgtcca ttcctttccc ggcgtcatac gcaaaatgcc gtccacattc
    23341 tgcttgatga tgccgtattc ctgcatctgc tttttcgctt cgccgtactt ctcatacagc
    23401 cgttcctttt cctcggccaa tcgcttgtac tcggctttca gggcgtacag atcggggagc
    23461 tttttcacac cctgtccctt tagggccttt gccgcagctt catacaaaat caacgtgctt
    23521 tcatgctcac gccggaattt tgccttatcc gctgcattgc ggtattccat agccacgggc
    23581 cagagctgct tgtaggtaga aatattcttt atcagcaccg ccatatccgc aagccggtgt
    23641 tccatagctt tgagggtagc ggcggcctcg tcgctggcgg cggtgatctc ggccactttg
    23701 ctttccaaat ccgggtaggt gtctatccca tgttcagtca ggaaattcaa agtctttgcg
    23761 gcctgcttca aattgtggag cttcgcccac ctctcatagc ctgccgactg ctgggcctta
    23821 atgttgtttt ccagatcaat ccgcaaagaa atccccttgc gctctttggg cgctctggtg
    23881 ataacgcggc ctttgatacg ctctttgatg gcttcctcgg tgtaggcttc ccccaatgtc
    23941 ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag aaataaactt tcccggcttg
    24001 atctcatagc cctgcgcctg catgagccgc aggaaatcat caaaatcttt tgcctgcggg
    24061 atagcggcgt caatggctgc tttgagcttc gctttccagc tcgtcccttt ccgctgggcg
    24121 tcccactcgg catagctctt gcccttactc tggccgggct tcaccacgga caatccgttt
    24181 tccttgcaca gcctgtcgct ggtgcgccgg atataggcat agctctgttt gttggaaatg
    24241 tacttgcgct gatttaccat atccacggca cagaaaataa tgtgattgtg gacgtggccc
    24301 ttatcaatat gggtagtcag cacataggga tatttccctt gcagcacttc ctcggcaagc
    24361 tgcttcccga tctcatgggc ctgttccggc gtggcttcgc cgggggcaaa ggattgtatc
    24421 aggtggtggc ccagattatt gcccttttgg tatgcctgcg ccagcagcat ttcaaattca
    24481 atgtccgctg tttcataaga gcaggcaaag gaggccacca gcagcttgcc gtcagttttg
    24541 tccgggttct cgatgtagtc aagggctttt ttcagcgttg acttgatagg atgtatcttt
    24601 gtaactgcca tatctgcgcc agcgccccct ttatatcctc gatgtcctgt gcgtacaccg
    24661 gccccatacc gttcagcctc tttgcaatct gattgacgtt gacgccgatc ttctgtatct
    24721 cggcggtctg cgcccggatg tcgctggtgt ccatgttgac gatatagccg tcaatgagca
    24781 tcttccgggc gtaggctgaa aagttcttcg tatgcagcag cgccattttc tgttcaatca
    24841 tagcccgttc ctccggcgtc acagggacgc gcagcacgat ttttcgcttt ctgtttgcca
    24901 tgcttcaccg tcctttccat tcaggggttt ggggatttcc ccaacaagca aaacggacgc
    24961 ggcccggcag ggacggggca ggccgttttt ccgggagtgg ctacactccc tatgcttgct
    25021 acgttactgt ttctccacta acaagacgtt tccaaagccc caaactaccc gcaaaatagc
    25081 cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc tgtgcagctc ccgctaaaag
    25141 taaaacagag gatgccagtt acacatcctc cgtttcattc ttcttcggct tttcaattag
    25201 tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat tcttcggggc agagtttcag
    25261 cttgtggctg accctttgcc gcagttcgct ttcctcccgc atgatctccg ggttaaagta
    25321 gcgttccaca ggaagcccgc aggctttaat aagctgtatg acaaccggca gactgggaat
    25381 ggcccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt
    25441 gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca
    25501 aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca
    25561 tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg
    25621 tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact
    25681 acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact
    25741 attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca
    25801 aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt
    25861 ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt
    25921 cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc
    25981 atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg
    26041 acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat
    26101 ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata
    26161 actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg
    26221 gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg
    26281 caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga
    26341 acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac
    26401 acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg
    26461 cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa
    26521 gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg
    26581 atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat
    26641 tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga
    26701 gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac
    26761 tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata cgaattctac
    26821 ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct gaggcaggat
    26881 atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac gaaaacactt
    26941 atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa cttttgcgtg
    27001 aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg gatgacgaaa
    27061 accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca ttcaagatgc
    27121 agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg gcaaaggaaa
    27181 tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt gacggtacag
    27241 agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag aacaaaggca
    27301 gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt gagggtgatg
    27361 gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa aatatcatca
    27421 aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa gaaaagggat
    27481 tttcattaag gttttttcct atacgatcat tgtcctgcta ctgcttgtcg gtgtaacagc
    27541 aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac agcaaacagt
    27601 aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg atattcaaga
    27661 ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg aagataaaga
    27721 gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta gacccaactt
    27781 tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaagca aggcaggtgt
    27841 gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga taatggttgt
    27901 attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga tcaaagcctt
    27961 agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc tggagcgaaa
    28021 ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc tgaaagaaac
    28081 catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa cacagcgata
    28141 tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa gcgttctgtt
    28201 ggagggaatg cttgaaaata tcggtgacta caaagatcat tctaagtatc tgcgcgaatg
    28261 catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc ttgtcagcct
    28321 gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca cggttgccga
    28381 gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg tcacagatat
    28441 tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac tatccaatgt
    28501 catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat ggagtgagcc
    28561 tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg atgatactgc
    28621 gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca gaaaaagtgg
    28681 gcgaagcaga aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa aaacgctgga
    28741 tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt tctggctgga
    28801 tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttgg gctctttgtc
    28861 aaataggact gatagagtga gataagaac
//
LOCUS       DADVXO010000351        17601 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.058, whole genome shotgun sequence.
ACCESSION   DADVXO010000351 DADVXO010000000
VERSION     DADVXO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.058"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            177..452
                     /locus_tag="KB950_003013"
     CDS             177..452
                     /locus_tag="KB950_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBB6859138.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            687..1733
                     /locus_tag="KB950_003014"
     CDS             687..1733
                     /locus_tag="KB950_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859139.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            1735..2994
                     /locus_tag="KB950_003015"
     CDS             1735..2994
                     /locus_tag="KB950_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6859140.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            3000..3581
                     /locus_tag="KB950_003016"
     CDS             3000..3581
                     /locus_tag="KB950_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBB6859141.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3663..4538)
                     /locus_tag="KB950_003017"
     CDS             complement(3663..4538)
                     /locus_tag="KB950_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6859142.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            4686..5018
                     /locus_tag="KB950_003018"
     CDS             4686..5018
                     /locus_tag="KB950_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6859143.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5130..6338
                     /locus_tag="KB950_003019"
     CDS             5130..6338
                     /locus_tag="KB950_003019"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBB6859144.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            6492..6947
                     /locus_tag="KB950_003020"
     CDS             6492..6947
                     /locus_tag="KB950_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859145.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            7309..7584
                     /locus_tag="KB950_003021"
     CDS             7309..7584
                     /locus_tag="KB950_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859146.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            7597..9675
                     /locus_tag="KB950_003022"
     CDS             7597..9675
                     /locus_tag="KB950_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBB6859147.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            9835..11721
                     /locus_tag="KB950_003023"
     CDS             9835..11721
                     /locus_tag="KB950_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6859148.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            11733..12680
                     /locus_tag="KB950_003024"
     CDS             11733..12680
                     /locus_tag="KB950_003024"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBB6859149.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            12700..13227
                     /locus_tag="KB950_003025"
     CDS             12700..13227
                     /locus_tag="KB950_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6859150.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13290..13943)
                     /locus_tag="KB950_003026"
     CDS             complement(13290..13943)
                     /locus_tag="KB950_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBB6859151.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            14075..14917
                     /locus_tag="KB950_003027"
     CDS             14075..14917
                     /locus_tag="KB950_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6859152.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            15075..15971
                     /locus_tag="KB950_003028"
     CDS             15075..15971
                     /locus_tag="KB950_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6859153.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            15974..16687
                     /locus_tag="KB950_003029"
     CDS             15974..16687
                     /locus_tag="KB950_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBB6859154.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            16707..17225
                     /gene="msrA"
                     /locus_tag="KB950_003030"
     CDS             16707..17225
                     /gene="msrA"
                     /locus_tag="KB950_003030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6859155.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            17222..17449
                     /locus_tag="KB950_003031"
     CDS             17222..17449
                     /locus_tag="KB950_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBB6859156.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT         6100 a         2985 c         3624 g         4892 t
ORIGIN      
        1 actaaaaaaa gcatgtgaaa attggtaaaa accttgctat taagcggttc atatgatatc
       61 gtgataacag aatattgaag ttaatcaata tttcttttta aacttctgga ccactcagaa
      121 agttttaact aaatgatatt tctatcatat ctcttgtgga ggaggtgaat ctatccatgg
      181 caaacaaagc agaattaatc gaaaaagttg cttcagctac tgatttaact aaaaaagatg
      241 caactgcagc agtagatgct gtattttcaa ctattcaaga tgctttagct aacggcgaaa
      301 aagttcaatt aatcggtttt ggtaactttg aagttcgcga acgcgcagct cgcaaaggtc
      361 gtaacccaca aactggtgaa gaaatcgaaa ttccagctag caaagtacct gcattcaaac
      421 caggtaaagc attaaaagac gctgttaaat aagacagttg aaaaagcgct tgtggctcta
      481 gggctacaag tgcttttctt tttatttcct agcagaaaag gaataaataa gagacagaga
      541 ttccgaatcc gtatgtcata catagaaaac acatgtattt tatttaattt ttatttattt
      601 gcaagttatg aggtgagctt tgctcttatc ttttctagaa tttgcttact aatggtaaaa
      661 taaaaataag aagaggaggg atgcgtatgg aacttgatta tcaagtagaa gatgcgttga
      721 cgaaagtgtt ttacggagaa acgctagtca gtcagctaag agaactttcc attgatcagc
      781 caatcttatt tttaacgaat cagcgctatt atgatttgtt tgcagataag atcaacccgt
      841 tatttgcaaa tcaagcaaac aatgactggt atatttgtgc caacactcaa tgcaatcatt
      901 taacagaatt aaagaacctg cttgatttcg tcaaacgcta tcccgaaaat caatccatgt
      961 ttatcatcgg atttggaaat gaagggatca tggatctggc aggttttttt caaaaacata
     1021 caacccttga ttcatcacta tggatgatcc ctgtatcgat ccgttctcta agccgagcat
     1081 taacaccgca gcggacgatt ttgcagctac caaatcgtgt agtgctgcaa accatcaatc
     1141 tgccagaacg catcgtttat gatcaaacaa tcagcgagcg gcaggtggac ggaaagcaga
     1201 ttgacttttt gacttttatc tgttgcggaa tgatttgtga tcatcgtttt ttgcaaaatc
     1261 tgtataaaaa ctatccaaat aaaaagcaat taacgactcg gccgtttgcc gggatgatcg
     1321 aagaaatgat ctatttttat caattagagg cagaaaacct gtcaaattat ggtaaactgt
     1381 ttgaacaagc tttttatctg accgaaaatg gccatctact ttctgctagt atgaaaaaat
     1441 ttttaggaat gatgtttcat ttcgtgtgga atctccaagc atatcctttt gatttccaac
     1501 taaaaaactt ttttgtgtgg ctggacttgc ttggctatcc aatccactgg ctggaacaga
     1561 tttcaaagat ggagtatttg gaaaaagttc tttttttggc agaaaaaagc aaaaaaatac
     1621 ttgttttaga aaaaataggt ataatagacg gataccaaca tccaaacgaa aaagaattat
     1681 tgagaacaat gacggtattt caacaaatcg tcagtgagat aagagggaat tgaaatgaat
     1741 acaaatagcg aaaaaatgtt gcaagcttta tcagatgaag acttgtcaca agcacaaatc
     1801 tttttagaaa aagcattaaa agaagaccca gcagatatat tagctgaact aggcgaagaa
     1861 ttgctggcta ttggcttttt gatcgaagca aaacagattt ttgaacaact gatcaaccaa
     1921 tacccagaaa atgacggact gaacatcccg ttagcagaaa ttgcaatcga agataatgag
     1981 atcgacttag cttttgaata cctggaaaaa gtggataagc aaagtgatta ctatccgcaa
     2041 agcttattgg cgatagctga tttgtaccaa gttatcggaa tccctgaagt gagtgaagca
     2101 aaattgaaag aagcagctgg gttgcttcct gatgaaccat tgatccagtt tgcgctagct
     2161 gaattgtatt tttcagttga tcgtttcaaa gaggcagcag tggtttatga aacattgttg
     2221 gcaagcggga taaatgaaat ttcaggaatc tcgatgcagg aacgtttagg acaatcctta
     2281 agtatgcaag gagaattcga agcagctatc gtacctttag aagaagcact gaaagaagaa
     2341 aggacagatg atcgactgtt ccagctagct tttacgaatc ttcaattgaa agaaaatcag
     2401 caggcaatca actatctgga agaacttcga gaagttaatc cgcattatca atcgttatat
     2461 ctatatctag gacaagctct tcaagaagaa gaaatgattg aagaagcaca aacagtgtta
     2521 gaagaaggaa tcaaagaaaa tccttaccaa gtcgaacttt atcacctggc ctcggaaaat
     2581 gctttccgtc tgcatgatag acagcgagca gaagaacttt tactgaaagc tttagaagta
     2641 ggagacaaac aagatgaaac attacttact ttaagtaatc tttacttgga tgatgaaaga
     2701 tatgaagatg tgatacaagt gatcaatcag atggaagaaa ctgctaatcc ttatgcagaa
     2761 tggaacctag cccatgctta taatgaatta gaagacttta ccgttgcagc tgtacactat
     2821 gaacaggctt atcatgaact atcacatgaa ccagatttct tgaaagagta tgcctttttc
     2881 ttaagagaag aagggcagct gaaaagagcg caagaattat tgacttatta tttaacactt
     2941 gaaccaggag atatggaggc actgtcatta ttagatgacc taacagagag gtgagaaaaa
     3001 tgctaattga tgtacgggaa aagaagaatt ttttaacgtg gatggtcaat catgtatctt
     3061 ttagccgaag agaagttttt tggatcttga attatcttgc caatcatgaa gcgattttaa
     3121 gcaatgtcca ctttgttgaa ggggcgaatt ttgcggatcg aggactgcag atcagtgatc
     3181 tatctgttga aggggaaccc atgaaattgt ttttgcaggg aaaagaattt acggacacag
     3241 accagatttt tcatgaaatc cgtttgaatt ggaaaaagcc tttatatgtg gaatgtatat
     3301 tcgaaaatgc ttggcagaca agagaatatc tatctatctt agaagacaat ccattttctt
     3361 cttgggattc ttcagtcagt gaagaagata ctgaattaat tgaacgatat tttcaagaaa
     3421 aagaagaaga ggcaaaacta aaaatgcttt atgctcaaat cgatcaagca ctagaagatg
     3481 gagataggga tgcttttttg aaattatctg atgaagttaa ccgaaagatc cttttgcaga
     3541 cacaaaagaa aagtaaccgg gtaagtgaac ataacaatta gttaaaaata gtatagtact
     3601 aaaaaagaca aaggtcagct ggatgtggaa tctttcctca tagcctatct ttgtcttttt
     3661 tcttatagtt ttttcagtaa tcttgtctgt ggcttcaaat aagtaaagcc ttgcccttcc
     3721 acttcatgca cattgatgat tgaaaggaaa gctttttcgt caatctcgtg gacgatcctc
     3781 ttagcttcag ttaattcact agagctaacg accatataga tgattttttt tgaattacca
     3841 gagaatgcgc cttctccaga aaggaaagtc acacctcttt gcagaccggt catcagtagt
     3901 ggtgcaatct cttcgctttt gttggagacg acaagtagac cttttgcaga atatccgcca
     3961 tctaagatcg aatcaatcac acgactgaaa acatacgaaa caatcaacgt atacatcata
     4021 cgtttcaagt cgatataggt taatgaagca agtaagacga tgatatcaaa tgccaatagg
     4081 gaacgcccca tagtgatgcc gtaattttgt tctaaaatcc gagcgataat atcactgccg
     4141 cctgttgttc cacccatgcg gtagacgatc ccgcttccta cacctgcaac taaacccgca
     4201 agtagagaaa cgatcagtag atcatgctgt aaattgattt ctagtggaac cctttgccag
     4261 atccataaaa agaatgacaa agaaagtgta cctagaacag tataggccaa ggaacgttta
     4321 ccgaggatct ttcctcctag taaaatcaat gggatgttga ttaataaagt cgtataagct
     4381 ggatcaatac caaataaagc tcttaagatc aaggtgatcc ctgagactcc cccttctgct
     4441 aaatgattag cgatgttcaa gtaaacaaga ccaaatgcat aaatacttgt tcctataatg
     4501 ataaaaagaa cttcttttac tgtttttcgt ttgaccatat gaattccagc tttcaattat
     4561 gtattttcac agaatttcca tttagaaatt ccttcctaaa tgtagcatgg aaattcgatt
     4621 ttctcaattg ctcttatcta ggatttgcgt taaagttttg gtaagatagt tttggaggga
     4681 aaaagatgga taaaaagcgt tctttataca gtatgcagca ggaagtggat gaatatatcc
     4741 agcaattcaa ggtggggtat ttctctccat tagctcagat ggctcgatta actgaagaag
     4801 taggtgaatt ggcaagagaa gtcaaccaca cttatggaga aaaaagcaaa aaggcttctg
     4861 aaccagttaa ctcggtagca gaagaacttg gtgatgtttt attcgttacg atgatcatgg
     4921 ctaattcatt gaatatcgac ttaactgaag tttttgaaaa aaacatggaa aaattcaatc
     4981 aaagagacca taatcgcttt gagcgtaagg acgagtaaac aagatgaagt ggtcctggcc
     5041 ttgaagactc tgaaacggac ttgtcaaaca gtcaccatgc aactgagtct ttatctcagg
     5101 acaagaaaca cgcaggaaag gttggaaaaa tgaaattaaa acaattacct ctggaatacc
     5161 aaaaggcaat tccagttctg aaaaaattag agaatgcagg atatgaagct tattttgtag
     5221 gtggaagtgt tcgtgatatc ttgctgaacc agccaatcca tgatgtggat atcgcaacaa
     5281 gcgcatttcc agaagaaatc aaacagcttt ttccaaaaac gatagatatt gggatcgaac
     5341 atggaactgt gttggtatta gaaaatgatg aacaatatga gatcaccaca tttcggacgg
     5401 aatcaactta tcaggactac cgtagaccag atcatgtcga attcgttcgt tcgttggcag
     5461 aagatttgaa aagaagagat tttacgatca atgcgtttgc tttgaaagaa gatggagaaa
     5521 tcatcgattt gttcgatggt ctgcaagatc tagaagaaca gatcttgcga gctgtaggca
     5581 atcctcatga gcggttccat gaagatgcct tacgaatgat gcgcgggttg cgttttgtca
     5641 gtcaactagg gttcaaatta gaggaagaaa ccttctacgc catcaaagaa aaccacgagc
     5701 tattaggtaa aatatctgta gaacgtataa atgtggaatt catcaagatg ctcttgggaa
     5761 atgaacgggc cgcgggaatc catgcattta ttgaaacaga gtgctacatt tgctgtcccg
     5821 gtttgaaaga agaaggcgaa gcacttttac gttttgctga attaccatcc gtcccattga
     5881 aagaagagag ccaagcatgg gcattattga tcagccaact ggatctaaaa gaaaatgaga
     5941 ttcgttcctt tttgaaatcg tggaagtgtt ctaaccaaat gattaaagac gttcaagcac
     6001 ttgtccaagg actacgacaa cgagaaactg gagctttgga accattcgaa ttgtacttct
     6061 taggcaaaaa acatgcactg caagtagaag aattgatgat ctattttgac gaaacacctc
     6121 agatggatgc cgtaggagaa tcctatgatc gtttacctat caagtcatta agcgaattag
     6181 cagtagatgg taaaatcttg ctagaagaaa ctggaaaaaa acctggtaag tgggttagtg
     6241 aagctttgca attagctgaa aaagccgtcg tagaacaaag aataaaaaat gataaaaaac
     6301 aggttttgga ttttttatcg aaagaaaaat tgatttaaaa aacactagac tgtatctgct
     6361 cagctttgtt ggctacttaa agtgggcaga tacgtcatta gaagaaaaaa cataagatgt
     6421 gaccacttac ataaatttgt gctaaactga ggtagtacaa gaataaatta catcataaag
     6481 gagtcttttt aatgagtaaa gaaatgacag cgctaaaatt ttattttaga aatggagaaa
     6541 cctggacgat caaccgccgt catatcggcg atctttggat caaacaaatc acaacaagtt
     6601 ttggacgaat caatggcagt gaatttgttg aaatccatcc gtgtgctgga tttaaaatcg
     6661 aaatcttcca tgaaggggac gcagttgcga cacatgatat caacctaggc ggactggaaa
     6721 tgggcatgtt taatcgcgca ttgaaatacg aagatatcga gcgaatggaa atcctttacc
     6781 gaaatggcac acctgatctt gtgtacttcc catatctaga taaaggaaca gaaggattag
     6841 acaatcaata tcaatcaaca aaaatcagcg aaaaaacagg taacttgtat atcgtgatca
     6901 atccagatca acgtgtagaa gatgtatacg gggaattttt cgaataaatc gatttttacg
     6961 caaaaatgag gctgtgaaag agatgtgtct tattactact gataggctgt ctcttccaca
     7021 ccgtttattc agaataaggg actgtgacaa gatttttgtc gcagccccat ttttttgttg
     7081 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
     7141 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
     7201 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
     7261 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
     7321 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
     7381 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
     7441 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
     7501 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
     7561 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
     7621 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
     7681 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
     7741 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
     7801 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
     7861 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
     7921 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
     7981 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     8041 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     8101 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     8161 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     8221 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     8281 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     8341 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     8401 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     8461 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     8521 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     8581 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     8641 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     8701 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     8761 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     8821 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     8881 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     8941 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     9001 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     9061 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     9121 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     9181 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     9241 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     9301 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     9361 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     9421 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     9481 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     9541 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     9601 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     9661 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     9721 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     9781 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     9841 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     9901 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     9961 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
    10021 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
    10081 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
    10141 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
    10201 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
    10261 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
    10321 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
    10381 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
    10441 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
    10501 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
    10561 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
    10621 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
    10681 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
    10741 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
    10801 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
    10861 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
    10921 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
    10981 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
    11041 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
    11101 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
    11161 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
    11221 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
    11281 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
    11341 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
    11401 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
    11461 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
    11521 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
    11581 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
    11641 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
    11701 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
    11761 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
    11821 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
    11881 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
    11941 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
    12001 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
    12061 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
    12121 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
    12181 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
    12241 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
    12301 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
    12361 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
    12421 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
    12481 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
    12541 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
    12601 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
    12661 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
    12721 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
    12781 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
    12841 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
    12901 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
    12961 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
    13021 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
    13081 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
    13141 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
    13201 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
    13261 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
    13321 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
    13381 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
    13441 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
    13501 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
    13561 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
    13621 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
    13681 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
    13741 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
    13801 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
    13861 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
    13921 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
    13981 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
    14041 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
    14101 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
    14161 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
    14221 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
    14281 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
    14341 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
    14401 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
    14461 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
    14521 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
    14581 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
    14641 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
    14701 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
    14761 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
    14821 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
    14881 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
    14941 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
    15001 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
    15061 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
    15121 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
    15181 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
    15241 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
    15301 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
    15361 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
    15421 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
    15481 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
    15541 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
    15601 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
    15661 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
    15721 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
    15781 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
    15841 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
    15901 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
    15961 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
    16021 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
    16081 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
    16141 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
    16201 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
    16261 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
    16321 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
    16381 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
    16441 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
    16501 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
    16561 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
    16621 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
    16681 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
    16741 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
    16801 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
    16861 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
    16921 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
    16981 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    17041 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    17101 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    17161 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    17221 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    17281 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    17341 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    17401 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    17461 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    17521 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    17581 gagggatgga ttgatagaga t
//
LOCUS       DADVXO010000352        13213 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.083, whole genome shotgun sequence.
ACCESSION   DADVXO010000352 DADVXO010000000
VERSION     DADVXO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.083"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(204..2162)
                     /locus_tag="KB950_003032"
     CDS             complement(204..2162)
                     /locus_tag="KB950_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6859157.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="KB950_003033"
     CDS             complement(2159..3031)
                     /locus_tag="KB950_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6859158.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="KB950_003034"
     CDS             complement(3373..4245)
                     /locus_tag="KB950_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6859159.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="KB950_003035"
     CDS             4373..4942
                     /locus_tag="KB950_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBB6859160.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="KB950_003036"
     CDS             5009..5746
                     /locus_tag="KB950_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6859161.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="KB950_003037"
     CDS             5977..7245
                     /locus_tag="KB950_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6859162.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="KB950_003038"
     CDS             7732..8196
                     /locus_tag="KB950_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6859163.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="KB950_003039"
     CDS             8180..8809
                     /locus_tag="KB950_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6859164.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="KB950_003040"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="KB950_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBB6859165.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="KB950_003041"
     CDS             10688..11134
                     /locus_tag="KB950_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBB6859166.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="KB950_003042"
     CDS             complement(11333..11848)
                     /locus_tag="KB950_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6859167.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="KB950_003043"
     CDS             11965..13167
                     /locus_tag="KB950_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6859168.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DADVXO010000353         8046 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.113, whole genome shotgun sequence.
ACCESSION   DADVXO010000353 DADVXO010000000
VERSION     DADVXO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.113"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(134..577)
                     /locus_tag="KB950_003044"
     CDS             complement(134..577)
                     /locus_tag="KB950_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBB6859169.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="KB950_003045"
     CDS             complement(795..2135)
                     /locus_tag="KB950_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6859170.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="KB950_003046"
     CDS             2404..3300
                     /locus_tag="KB950_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBB6859171.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="KB950_003047"
     CDS             complement(3450..4391)
                     /locus_tag="KB950_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBB6859172.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="KB950_003048"
     CDS             4517..5638
                     /locus_tag="KB950_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6859173.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="KB950_003049"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="KB950_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBB6859174.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="KB950_003050"
     CDS             7426..7992
                     /locus_tag="KB950_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6859175.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2669 a         1503 c         1593 g         2281 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagc
//
LOCUS       DADVXO010000354         5615 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.142, whole genome shotgun sequence.
ACCESSION   DADVXO010000354 DADVXO010000000
VERSION     DADVXO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.142"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            86..778
                     /locus_tag="KB950_003051"
     CDS             86..778
                     /locus_tag="KB950_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6859176.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1105..1602
                     /gene="dfrG"
                     /locus_tag="KB950_003052"
     CDS             1105..1602
                     /gene="dfrG"
                     /locus_tag="KB950_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBB6859177.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1674..3626)
                     /locus_tag="KB950_003053"
     CDS             complement(1674..3626)
                     /locus_tag="KB950_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBB6859178.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3633..3869)
                     /locus_tag="KB950_003054"
     CDS             complement(3633..3869)
                     /locus_tag="KB950_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859179.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4271..4561
                     /locus_tag="KB950_003055"
     CDS             4271..4561
                     /locus_tag="KB950_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859180.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5275..5577
                     /locus_tag="KB950_003056"
                     /pseudo
     CDS             5275..5577
                     /locus_tag="KB950_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1904 a          905 c          979 g         1827 t
ORIGIN      
        1 catatgtaat ttaaaaacgc ttgacattgt gtttgagatc acttactttt agcatgtaaa
       61 agtattggat taaggagcta tgcaaatgaa aaagaaattt attactggtg ggattgtcgt
      121 tgcatttcta tgtctaattg gctacacagg cacgcggtac gcaaatgaaa ttattttgtg
      181 gggtggagag acagatattg atacaatcaa tgagaatctg acacagcttg atcatacatt
      241 gacagataaa gagcaagcga ttacagatct taccgctcga ctagcacaaa aagaacagac
      301 attgcaagat tcaaaggcag atgtccaaga ataccaaaca aaagtaaatg aattagaaaa
      361 ccaaaaaaat caatcggcag cagaaaaaca aaacttagaa agtcaattag tcagcaagga
      421 tgcacaacta caaagcaaac aaaatgaaat caatgtcaaa ctagatgaga tcaaccaaaa
      481 aaatgatgaa atcaatcatc tgaatgcaga ttggacagca agactacaag aagcacaagc
      541 aaaactttca gaattacaaa atactgtaaa tggtttgaat caacaaatcg caacactgac
      601 acaagaaaaa acaaatgtag aaactcagtt aacagataca caaaagaaat tagacgaagc
      661 aactgctgaa aactcaagct ggaaacaata tattcaaaag ttagaaaaag caaaagatga
      721 agtggaagat acagctgata aatctcagca aattgttgaa gaacatttgc gtaaataatc
      781 tttcttaaca agtaaaccta aattagtaaa gaagcaacag taatctatac taagtagttc
      841 agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac
      901 ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat
      961 gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca
     1021 actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa
     1081 ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat
     1141 agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt
     1201 aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga atcaatcgga
     1261 agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat
     1321 ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa
     1381 atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg
     1441 tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat
     1501 gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac
     1561 tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag
     1621 aagaacagta aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg
     1681 aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt
     1741 gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga
     1801 attgtaatcg caaattgagc atacttcgga ttgaagttag cgtaaatata gctcttctta
     1861 tcaccacgag ctgttgttaa tggtctttca agagatgata tataacgtct tatctgttga
     1921 ataaagctat tggtcgaatg gtcattaaca tttaatagca tctttgcgat ttcgtttggt
     1981 tccagagcag ataaatccgt tctacaatca actgaataaa atcctttatc cttagtggct
     2041 aatggatgtt tgatagggtt atcggcatat ttgcgatatc tttctccatc tttactaata
     2101 gcctcttcgt ggaattgatg acttgttagt aattcagtta gataatgaga tgccaaagtc
     2161 cgtagaaact ttgttttgaa tcctctgata tctccccaat cgagcagttc tgcttttgct
     2221 tgtccgaatt cttttaagca ttgttttctg cttttcgtct tgttaacttg gcaaataaaa
     2281 tgatgtgcat cgctcaacct taattctttc gtgaatactc tataaaaggc gtttctaata
     2341 gagaagtcat catcactaat cattcgccat tcgtcggcat taaccatttg tttaattaac
     2401 caatagtgag cagttgtagt ataaggagca tcaacattta aaccatcgac aaatctactc
     2461 ctattagtaa ttttccctaa ttcgtgaaga taagcattcc tattttcgct atcgttagcg
     2521 gatggttctt gtggataata agaccaatct aacctatcat ttttacgact aaaagtattg
     2581 agatggtctt ctttgaattt tcttgtatct tcatttaatt catccattga aatattccaa
     2641 tcataggcaa catcagaacg gaacacatat cttgataaga cctcagcaga tacaactata
     2701 tacgtttgca tattaaggtc ttcaaacccc gtatatttct ttgaaccttg tcctttctta
     2761 cggacgttat ttaggtagta gtgcatcttg tccgtattaa gccacatctc gttaaatttc
     2821 tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg cctatacagc
     2881 cactccagct tatgatagta ggttccaact ccaattccta aaatatcaca agtacgactg
     2941 actgaaaccc ttccaacaac catcttagcg aacattggaa gaatggtgtt cttttgttgg
     3001 tgataagtag tggtttcttc tctttttggt agaacattag tgaacttctt acacgcctta
     3061 cattgatagc gttgagattt gcccctactt ttcccccttt tgtaaaattg cttcggctca
     3121 ttgaatgggg ttgattcttc ttccgagcag ccttctttat gaaattgata atctggctca
     3181 acatcttgga tgctattgat ttctataagg cgtttaattt cttcaacaac agaccaatta
     3241 gataaagggg ttactgtaca gttttgcgat acacctctgc caataggatt gtcattgcag
     3301 tacatacctt tatgaccttt atctttggaa ctaccagtca ttttatatcg acttggtttc
     3361 ccttttacat ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa cggattgatg
     3421 cagtaattat attgaatggg atattcagaa ccgttccatt taaaagacgt tggcaagaac
     3481 aacattttgt tataccttgt tgcaaatcgt tttggagtta ataaatcata ttgtttagcc
     3541 ctatctttta attcttcgtt tgaaatcgga attttaacat ctacaatttc atcttccttc
     3601 gtggctaatc ttttaagctt ggtcattgtc tatcaaccca ctctatcttt gatacccatt
     3661 ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc attcaataaa cttcttatcg
     3721 tctaaaatgt tcaataagaa ttcatccact gcttcgcttt cagtacgctc tgcatattca
     3781 gcgtattgct taactatcac ccttacttgc tctgatattt cccaatccac agacttagcg
     3841 ttcttatttt ttggctcaag aaattgcata ggaaaatgca cccctttttg cttagtaaat
     3901 tgtaaccatt ttaacacacc aatttgcatt cctacaataa ttatttcacg ttcgcatttt
     3961 tatatacatt ttaacttaca ttttgtggtg taaattaaca acaaacgttg atttaacaat
     4021 aaaaaacgag tatcaaattc agttgcaatt tgatactcat ttaggtttac attttcactt
     4081 tttgtactcc aaaactgaac tacttaaatc tatactgctg cctctttttt ttagtacatg
     4141 tccttcatct tcgcaggaag tgccaatatg attttaagaa aggtaataga caaaaagtca
     4201 aaaagataaa acagagtttc ttctttacaa agggaatgta tgttcgtata atggtattga
     4261 ggtgaaggtt atggagaaaa aatatggaac agtagataag atccgtactt tgcaagttag
     4321 tgccagaccg ttgatccgtt tcagtttaaa tggggtcaat tgtttgattg cttcccatag
     4381 tttgaacttc ctagctgaag tagacgaagg gatgaagtta gtcgttacag gttattataa
     4441 taatagaaag caatttgttg tgagatccta tcatgtgtta gggaaaccca aaattgtggt
     4501 agaatatgag aaaagtctat acccaagaaa aaaggtccga caagatgaaa gaataaacta
     4561 gaaaacaagc ggctgagaca aaaatgtcct agccgcttgt tttggaaaga tggatgcaga
     4621 cgatgggatt tgaacccaca cgagcatacg ctcacaagaa cctcaatcta tatataacta
     4681 catgtattta agtatttgtg atgaatcact aaactttcca aactcatttg agggatattg
     4741 cttaaagatt gaacagatca attcgaagcc ctattagtat tcgctcaatg gggggatcag
     4801 aggccataaa aagagactag tcattactag tctcaggtag tggaattgta ggattttaaa
     4861 cgttcctgga gagtaccggt atgtaactcg aagaggtgat tatcatagtc gtaaaagtaa
     4921 attgaagaag cctcgccttt cacacgagaa cgtcctggtt cgacagttaa acctaacatt
     4981 tggatttttg agacaaacga atctatatct tgttcgtcaa ttttaaaagc aatatgattg
     5041 tatgtctttg gcagtttatt cgacgaattc tgcattactg caatccatag gtccttaact
     5101 aagaaaaatt tttctggata taatgaaaat ttttttgttg actatcgtag atttcttttg
     5161 catcaaataa ggtatttaac aattaagtat ttaaaagata acttttaagt tatctttaag
     5221 taatgcttaa agctatgtta tactctatta ggaaatataa gaaggagtat aaaaatgaac
     5281 aaacataata gaaaaataac gaataacaaa gaactgatct tgttactaat agggatttgg
     5341 ggtatctttt tactctttaa tatgtatatg ccgactatga gagcggatga cgtagtttat
     5401 gcaagtagat tagatgaact tggctatttg ggtgcttcga tagaacatta taagacatgg
     5461 agttcaagaa ttattataga actattctta atgttctttt ctaaacactt tatgctatgg
     5521 aaattattaa attctacgat tatgttagga attgtagtat tactttgcaa gtatgttgag
     5581 agtataaaat attttgtgta aatgaaaaaa tccat
//
LOCUS       DADVXO010000355         4702 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.161, whole genome shotgun sequence.
ACCESSION   DADVXO010000355 DADVXO010000000
VERSION     DADVXO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.161"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            194..1000
                     /locus_tag="KB950_003057"
     CDS             194..1000
                     /locus_tag="KB950_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBB6859181.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1725
                     /locus_tag="KB950_003058"
     CDS             1018..1725
                     /locus_tag="KB950_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBB6859182.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1722..2693
                     /locus_tag="KB950_003059"
     CDS             1722..2693
                     /locus_tag="KB950_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBB6859183.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2686..3714
                     /gene="vanB"
                     /locus_tag="KB950_003060"
     CDS             2686..3714
                     /gene="vanB"
                     /locus_tag="KB950_003060"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBB6859184.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3720..4328
                     /locus_tag="KB950_003061"
     CDS             3720..4328
                     /locus_tag="KB950_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBB6859185.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4394..4567)
                     /locus_tag="KB950_003062"
     CDS             complement(4394..4567)
                     /locus_tag="KB950_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6859186.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1329 a          986 c         1245 g         1142 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg
     1321 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1381 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1441 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1501 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1561 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1621 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1681 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1741 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1801 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1861 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     1921 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     1981 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2041 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2101 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2161 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2221 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2281 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2341 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2401 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2461 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2521 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2581 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2641 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2701 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2761 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2821 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     2881 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     2941 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3001 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3061 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3121 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3181 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3241 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3301 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3361 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3421 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3481 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3541 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3601 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3661 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3721 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3781 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3841 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     3901 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     3961 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4021 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4081 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4141 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4201 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4261 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4321 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4381 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4441 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4501 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4561 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4621 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
     4681 gttgaactat ttaatggact gt
//
LOCUS       DADVXO010000356         2771 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.200, whole genome shotgun sequence.
ACCESSION   DADVXO010000356 DADVXO010000000
VERSION     DADVXO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.200"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            110..193
                     /locus_tag="KB950_003063"
     CDS             110..193
                     /locus_tag="KB950_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB6859187.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="KB950_003064"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="KB950_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB6859188.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="KB950_003065"
     CDS             complement(1318..2181)
                     /locus_tag="KB950_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6859189.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="KB950_003066"
     CDS             complement(2183..2455)
                     /locus_tag="KB950_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6859190.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="KB950_003067"
     CDS             complement(2472..2681)
                     /locus_tag="KB950_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBB6859191.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DADVXO010000357         2657 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301364-19
            SAMEA8426448-rid11941463.guided.207, whole genome shotgun sequence.
ACCESSION   DADVXO010000357 DADVXO010000000
VERSION     DADVXO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426448
            Sequence Read Archive: ERR5555413
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005862.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005862.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:18:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,010
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 28 of 63
            Pseudo Genes (internal stop)      :: 16 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426448-rid11941463.guided.207"
                     /isolate="301364-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..196
                     /locus_tag="KB950_003068"
     CDS             <1..196
                     /locus_tag="KB950_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBB6859192.1"
                     /translation="NLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHGT
                     DIDAACGQLRSKQMKKTVAK"
     gene            complement(389..1492)
                     /locus_tag="KB950_003069"
     CDS             complement(389..1492)
                     /locus_tag="KB950_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6859193.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2044..2592
                     /gene="aac(6')"
                     /locus_tag="KB950_003070"
     CDS             2044..2592
                     /gene="aac(6')"
                     /locus_tag="KB950_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBB6859194.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          864 a          465 c          514 g          814 t
ORIGIN      
        1 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
       61 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
      121 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
      181 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
      241 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
      301 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
      361 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
      421 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
      481 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
      541 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
      601 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
      661 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
      721 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
      781 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
      841 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
      901 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
      961 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
     1021 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
     1081 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
     1141 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
     1201 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
     1261 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
     1321 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
     1381 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
     1441 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
     1501 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
     1561 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
     1621 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
     1681 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
     1741 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
     1801 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
     1861 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttat
     1921 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
     1981 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
     2041 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
     2101 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
     2161 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
     2221 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
     2281 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
     2341 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
     2401 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
     2461 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
     2521 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
     2581 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
     2641 atttacgaaa attactt
//
